Next Article in Journal
The Role of Alternative Splicing in the Control of Immune Homeostasis and Cellular Differentiation
Previous Article in Journal
The Development of Neuroendocrine Disturbances over Time: Longitudinal Findings in Patients after Traumatic Brain Injury and Subarachnoid Hemorrhage
Previous Article in Special Issue
Effects of PMMA and Cross-Linked Dextran Filler for Soft Tissue Augmentation in Rats
 
 
Article

Poly-ε-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects

1
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover D-30559, Germany
2
Division of Medicine Clinic III, Hematology, Oncology and Palliative Medicine, University of Rostock, Rostock D-18057, Germany
3
Institute for Biomedical Engineering, Rostock University Medical Center, Rostock D-18119, Germany
4
Materials and Processes Department, Laser Zentrum Hannover e. V., Hannover D-30419, Germany
5
Clinic for Cranio-Maxillo-Facial Surgery, Hannover Medical School, Hannover D-30625, Germany
6
Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Foundation, Hannover D-30559, Germany
7
Institut fuer Werkstoffkunde, Leibniz Universitaet Hannover, Garbsen D-30823, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Mohamed N. Rahaman
Int. J. Mol. Sci. 2016, 17(1), 1; https://doi.org/10.3390/ijms17010001
Received: 28 October 2015 / Revised: 10 December 2015 / Accepted: 14 December 2015 / Published: 22 December 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
For healing of critically sized bone defects, biocompatible and angiogenesis supporting implants are favorable. Murine osteoblasts showed equal proliferation behavior on the polymers poly-ε-caprolactone (PCL) and poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate) (P(3HB)/P(4HB)). As vitality was significantly better for PCL, it was chosen as a suitable coating material for further experiments. Titanium implants with 600 µm pore size were evaluated and found to be a good implant material for bone, as primary osteoblasts showed a vitality and proliferation onto the implants comparable to well bottom (WB). Pure porous titanium implants and PCL coated porous titanium implants were compared using Live Cell Imaging (LCI) with Green fluorescent protein (GFP)-osteoblasts. Cell count and cell covered area did not differ between the implants after seven days. To improve ingrowth of blood vessels into porous implants, proangiogenic factors like Vascular Endothelial Growth Factor (VEGF) and High Mobility Group Box 1 (HMGB1) were incorporated into PCL coated, porous titanium and magnesium implants. An angiogenesis assay was performed to establish an in vitro method for evaluating the impact of metallic implants on angiogenesis to reduce and refine animal experiments in future. Incorporated concentrations of proangiogenic factors were probably too low, as they did not lead to any effect. Magnesium implants did not yield evaluable results, as they led to pH increase and subsequent cell death. View Full-Text
Keywords: titanium implants; angiogenesis; poly-ε-caprolactone; VEGF; HMGB1; poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate) titanium implants; angiogenesis; poly-ε-caprolactone; VEGF; HMGB1; poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate)
Show Figures

Figure 1

MDPI and ACS Style

Roland, L.; Grau, M.; Matena, J.; Teske, M.; Gieseke, M.; Kampmann, A.; Beyerbach, M.; Murua Escobar, H.; Haferkamp, H.; Gellrich, N.-C.; Nolte, I. Poly-ε-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects. Int. J. Mol. Sci. 2016, 17, 1. https://doi.org/10.3390/ijms17010001

AMA Style

Roland L, Grau M, Matena J, Teske M, Gieseke M, Kampmann A, Beyerbach M, Murua Escobar H, Haferkamp H, Gellrich N-C, Nolte I. Poly-ε-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects. International Journal of Molecular Sciences. 2016; 17(1):1. https://doi.org/10.3390/ijms17010001

Chicago/Turabian Style

Roland, Laura, Michael Grau, Julia Matena, Michael Teske, Matthias Gieseke, Andreas Kampmann, Martin Beyerbach, Hugo Murua Escobar, Heinz Haferkamp, Nils-Claudius Gellrich, and Ingo Nolte. 2016. "Poly-ε-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects" International Journal of Molecular Sciences 17, no. 1: 1. https://doi.org/10.3390/ijms17010001

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop