Next Article in Journal
Structural Basis for Carbapenem-Hydrolyzing Mechanisms of Carbapenemases Conferring Antibiotic Resistance
Next Article in Special Issue
Regulative Effect of Mir-205 on Osteogenic Differentiation of Bone Mesenchymal Stem Cells (BMSCs): Possible Role of SATB2/Runx2 and ERK/MAPK Pathway
Previous Article in Journal
A Fusion Protein of RGD4C and β-Lactamase Has a Favorable Targeting Effect in Its Use in Antibody Directed Enzyme Prodrug Therapy
Previous Article in Special Issue
Micro RNA-124a Regulates Lipolysis via Adipose Triglyceride Lipase and Comparative Gene Identification 58
Open AccessArticle

The miRNA Transcriptome Directly Reflects the Physiological and Biochemical Differences between Red, White, and Intermediate Muscle Fiber Types

by Jideng Ma 1,2,†, Hongmei Wang 1,2,†, Rui Liu 1,2, Long Jin 1,2, Qianzi Tang 1,2, Xun Wang 1,2, Anan Jiang 1,2, Yaodong Hu 1,2, Zongwen Li 3, Li Zhu 1,2, Ruiqiang Li 3, Mingzhou Li 1,2,* and Xuewei Li 1,2,*
1
Institute of Animal Genetics & Breeding, College of Animal Science & Technology, Sichuan Agricultural University, Ya’an 625014, Sichuan, China
2
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, Sichuan, China
3
Novogene Bioinformatics Institute, Beijing 100083, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work
Academic Editor: Martin Pichler
Int. J. Mol. Sci. 2015, 16(5), 9635-9653; https://doi.org/10.3390/ijms16059635
Received: 8 January 2015 / Revised: 24 March 2015 / Accepted: 13 April 2015 / Published: 29 April 2015
(This article belongs to the Collection Regulation by Non-Coding RNAs)
MicroRNAs (miRNAs) are small non-coding RNAs that can regulate their target genes at the post-transcriptional level. Skeletal muscle comprises different fiber types that can be broadly classified as red, intermediate, and white. Recently, a set of miRNAs was found expressed in a fiber type-specific manner in red and white fiber types. However, an in-depth analysis of the miRNA transcriptome differences between all three fiber types has not been undertaken. Herein, we collected 15 porcine skeletal muscles from different anatomical locations, which were then clearly divided into red, white, and intermediate fiber type based on the ratios of myosin heavy chain isoforms. We further illustrated that three muscles, which typically represented each muscle fiber type (i.e., red: peroneal longus (PL), intermediate: psoas major muscle (PMM), white: longissimus dorsi muscle (LDM)), have distinct metabolic patterns of mitochondrial and glycolytic enzyme levels. Furthermore, we constructed small RNA libraries for PL, PMM, and LDM using a deep sequencing approach. Results showed that the differentially expressed miRNAs were mainly enriched in PL and played a vital role in myogenesis and energy metabolism. Overall, this comprehensive analysis will contribute to a better understanding of the miRNA regulatory mechanism that achieves the phenotypic diversity of skeletal muscles. View Full-Text
Keywords: miRNA; fiber type; pig; myogenesis; energy metabolism miRNA; fiber type; pig; myogenesis; energy metabolism
Show Figures

Figure 1

MDPI and ACS Style

Ma, J.; Wang, H.; Liu, R.; Jin, L.; Tang, Q.; Wang, X.; Jiang, A.; Hu, Y.; Li, Z.; Zhu, L.; Li, R.; Li, M.; Li, X. The miRNA Transcriptome Directly Reflects the Physiological and Biochemical Differences between Red, White, and Intermediate Muscle Fiber Types. Int. J. Mol. Sci. 2015, 16, 9635-9653.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop