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Int. J. Mol. Sci. 2015, 16(12), 28242-28254;

ITSN2L Interacts with and Negatively Regulates RABEP1

Key Laboratory of Protein Chemistry and Developmental Biology of State Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410081, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 26 September 2015 / Revised: 28 October 2015 / Accepted: 2 November 2015 / Published: 27 November 2015
(This article belongs to the Section Biochemistry)
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Intersectin-2Long (ITSN2L) is a multi-domain protein participating in endocytosis and exocytosis. In this study, RABEP1 was identified as a novel ITSN2L interacting protein using a yeast two-hybrid screen from a human brain cDNA library and this interaction, specifically involving the ITSN2L CC domain and RABEP1 CC3 regions, was further confirmed by in vitro GST (glutathione-S-transferase) pull-down and in vivo co-immunoprecipitation assays. Corroboratively, we observed that these two proteins co-localize in the cytoplasm of mammalian cells. Furthermore, over-expression of ITSN2L promotes RABEP1 degradation and represses RABEP1-enhanced endosome aggregation, indicating that ITSN2L acts as a negative regulator of RABEP1. Finally, we showed that ITSN2L and RABEP1 play opposite roles in regulating endocytosis. Taken together, our results indicate that ITSN2L interacts with RABEP1 and stimulates its degradation in regulation of endocytosis. View Full-Text
Keywords: ITSN2L; RABEP1; endocytosis; endosome; interactions ITSN2L; RABEP1; endocytosis; endosome; interactions

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Yang, X.; Yan, F.; He, Z.; Liu, S.; Cheng, Y.; Wei, K.; Gan, S.; Yuan, J.; Wang, S.; Xiao, Y.; Ren, K.; Liu, N.; Hu, X.; Ding, X.; Hu, X.; Xiang, S. ITSN2L Interacts with and Negatively Regulates RABEP1. Int. J. Mol. Sci. 2015, 16, 28242-28254.

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