Comprehensive Investigation of Ginsenosides in the Steamed Panax quinquefolius with Different Processing Conditions Using LC-MS

Panax quinquefolius (PQ) has been widely used in traditional Chinese medicine and functional food. Ginsenosides are the important functional components of PQ. The ginsenosides’ diversity is deeply affected by the processing conditions. The ginsenosides in the steamed PQ have been not well-characterized yet because of the complexity of their structure. In the study, the comprehensive investigation of ginsenosides was performed on the steamed PQ with different steaming times and temperatures by UPLC-Q-TOF-MS. Based on the molecular weight, retention time and characterized fragment ions, 175 ginsenosides were unambiguously identified or tentatively characterized, including 45 protopanaxatriol type, 49 protopanaxadiol type, 19 octillol type, 6 oleanolic acid type ginsenosides, and 56 other ginsenosides. Ten new ginsenosides and three new aglycones were discovered in the steamed PQ samples through searching the database of CAS SciFindern. Principal component analysis showed the significant influence on the chemical components of PQ through different processing conditions. The steaming temperature was found to promote the transformation of ginsenosides more than the steaming time. The protoginsenosides were found to transform into the rare ginsenosides by elimination reactions. The malonyl ginsenosides were degraded into acetyl ginsenosides, and then degraded into neutral ginsenosides. The sugar chain experienced degradation, with position changes and configuration inversions. Furthermore, 20 (S/R)-ginsenoside Rh1, Rh2, Rg2, and Rh12 were found to transform from the S-configuration to the R-configuration significantly. This study could present a comprehensive ginsenosides profile of PQ with different steaming conditions, and provide technical support for the development and utilization of PQ.


Introduction
Panax quinquefolius (PQ) belongs to the genus Panax in the family Araliaceae.It has been widely used in traditional Chinese medicine, dietary supplements, and functional food [1,2].It is native to the south of Canada and the northern USA, and has been widely planted in China.In vivo and in vitro studies have shown that PQ has many biological activities, including antioxidative, antidiabetic, anti-inflammatory, anti-cancer, etc., [3,4].It has been demonstrated to have a positive impact on the treatment of various diseases such as in the central nervous system, endocrine system, cardiovascular system, as well as cancer [5].In particular, PQ has unique advantages in treating chronic diseases because of its better compatibility with the human body and fewer side effects [3].It was found that steamed PQ has been reported to exhibit enhanced antiproliferative activity and antioxidant capacity [6,7].PQ contains many chemical components, including ginsenosides, polyacetylenes, polyphenolic compounds, etc. Ginsenosides are the most important active ingredients [8,9].More than 500 ginsenosides have been discovered from Panax species [10], but only a few have been characterized in the steamed PQ samples.A comprehensive investigation of ginsenosides is essential for the further development and utilization of the steamed PQ.
Based on the structure difference of the aglycone, ginsenosides are divided into protopanaxadiol (PPD-type), protopanaxatriol (PPT-type), octillol (OT-type), oleanolic (OA-type), and other types [11].OT-type ginsenosides are characteristic in the PQ, differing from other Panax species [12].The ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 are generally considered as the main protoginsenosides.Protoginsenosides have a larger molecular structure that is difficult to be absorbed by the human body, whereas less-polar ginsenosides are easily absorbed by the intestinal microflora [13,14].These ginsenosides are called rare ginsenosides, and they have been found to have special pharmacological activities [15].Compared to ginsenoside Rb1, ginsenoside Rg5 has been demonstrated to have stronger antiproliferative activity against breast cancer [16,17].Ginsenoside Rh4 has been reported to have anti-esophageal cancer effects through inhibiting aerobic glycolysis [18,19].Rare ginsenosides can be prepared by physical, chemical, and biological methods [20].The chemical method has usually the disadvantage of a long reaction time and the production of byproducts.The biotransformation of rare ginsenosides needs to solve the problems of low specific activities, unidentified enzymes, and uncovered catalytic mechanisms [21].The physical method commonly refers to the steaming method.It is a green and efficient method to regulate the transformation of ginsenosides [22].Ginsenosides undergo chemical modifications and generate rare ginsenosides during the steaming process [23].There have been several reports on steamed PQ.Twelve ginsenosides were determined in the roots of steamed PQ, and the anticancer activities of the extract from roots steamed for 2 h were greater than 1 h [24].Twenty-nine major ginsenosides have been studied in the multisteamed PQ samples and a possible chemical conversion was deduced [25].Fifty-nine ginsenosides of PPT, PPD, OA, and OT types have been analyzed in PQ with different steaming processes [26].However, a few known ginsenosides cannot meet the needs of holistic studies or the discovery of chemical markers in the PQ steaming process [27].Further studies of the steam processing mechanism will be of great significance for the rational utilization of PQ.
The development of analytical technologies has made it possible for an holistic study.LC-MS has been widely used in secondary metabolite analysis for its high sensitivity and high throughput, such as for flavonoids, phenolic acids, and ginsenosides [28,29].In the study, ginsenosides profiling has been established for the steamed PQ samples based on LC-MS.The in-depth identification of PQ was performed, the aglycone and the sugar chains were annotated, and new ginsenosides were discovered.The structure and content changes of the ginsenosides were analyzed with the different steaming processes, and the transformation mechanisms of ginsenosides were further investigated.The study could present the comprehensive ginsenoside profiling of steamed PQ with different times and temperatures.

Identification of Ginsenosides in PQ Samples
The total ion chromatogram of the PQ sample with LC-MS is shown in Figure 1.For ginsenosides, there were specific fragmentation rules in the MS/MS analysis [30].The identification of ginsenosides was based on the accurate molecular weight, retention time, and MS/MS fragment ions.With different collision energies in the MS/MS analysis, ginsenosides were fragmented into aglycone and sugar chains.Characterized aglycone ions, sugar chain ions, and the neutral loss of sugar chains were found.For the PPT-, PPD-, OT-, or OA-type, the characterized aglycone ions were 475, 459, 491, and 455 (m/z), respectively.The chemical structures of the PPT-, PPD-, OT-, or OA-type aglycones are shown in Figure 2 1.
ions, sugar chain ions, and the neutral loss of sugar chains were found.For the PPT-, PPD-, OT-, or OA-type, the characterized aglycone ions were 475, 459, 491, and 455 (m/z), respectively.The chemical structures of the PPT-, PPD-, OT-, or OA-type aglycones are shown in Figure 2 In addition, acetyl and malonyl ginsenosides were characterized as having a neutral loss of 42 and 86.Then, a total of 175 ginsenosides were identified from the extracts of the PQ samples, which included 45 PPT-type, 49 PPD-type, 19 OT-type, 6 OA-type ginsenosides, and 56 other ginsenosides.The ginsenosides were then validated with the standards available.The detailed molecular weight, retention time, aglycone, sugar chains, and MS/MS fragment ions of the ginsenosides are listed in Table 1.In addition, acetyl and malonyl ginsenosides were characterized as having a neutral loss of 42 and 86.Then, a total of 175 ginsenosides were identified from the extracts of the PQ samples, which included 45 PPT-type, 49 PPD-type, 19 OT-type, 6 OA-type ginsenosides, and 56 other ginsenosides.The ginsenosides were then validated with the standards available.The detailed molecular weight, retention time, aglycone, sugar chains, and MS/MS fragment ions of the ginsenosides are listed in Table 1.For the structures of the ginsenosides, the database CAS SciFinder n was searched.Among them, 10 ginsenosides were identified in the steamed PQ for the first time.The aglycone or sugar chains were different compared with the ginsenosides reported previously.There was one new ginsenoside classified as the PPT-type, and it was named PPT-O-glc-rha/O-rha.PPT-O-glc-rha/O-rha (m/z 975.5534, [M+HCOO] − ) was identified by fragment ions of 929, 783, 621, 475, 205, 163, 161, and 145 (Figure 3A).The fragment ion of 475 is the characteristic aglycone ion of PPT-type ginsenosides.The neutral loss of 929/783, 783/621, and 621/475 indicated two rhamnose and a glucose in the sugar chains.The fragment ion of 205 indicated a sugar chain of O-glucose-rhamnose.
There were nine new ginsenosides classified as the other type, and they were named PQ-ginsenoside A, B, C, D, acetyl-PQ-ginsenoside A, and acetyl-PQ-ginsenoside D isomers.Three new aglycones were discovered in PQ-ginsenoside B, C, and D, and the m/z of them were 449, 431, and 415 in the MS/MS analysis.For example, the fragmentation rules of PQginsenoside C were consistent with the general ginsenosides, and a new aglycone with m/z 431 was discovered.The m/z of the aglycone in PQ-ginsenoside A was 433.The aglycone with m/z 433 has been reported to be degraded from 459 in the PPD-type, and it was characterized as 25-, 26-, and 27-trinor-PPD-type in the floralginsenoside Kb from Panax ginseng [31,32].The sugar chain of PQ-ginsenoside A was different from floralginsenoside Kb, and it was not found in the database of CAS SciFinder n , so it was defined as a new ginsenoside.The sugar chains of PQ-ginsenoside A were the same as Rg3, and they were eluted at the same retention time.Rg3 is a PPD-type ginsenoside.Therefore, the aglycone structure in PQ-ginsenoside A were deduced as 25-, 26-, and 27-trinor-PPD-type degraded from Rg3 (Figure 3B).For PQ-ginsenoside B (aglycone, m/z, 449, Figure 3C), it has the same sugar chains and retention time with Rg2 (aglycone, m/z, 475).For PQ-ginsenoside C (aglycone, m/z, 431, Figure 3D), it has the same sugar chains and retention time with Rg6 (aglycone, m/z, 457).For PQ-ginsenoside D (aglycone, m/z, 415, Figure 3E) it has the same sugar chains and retention time with Rg5 (aglycone, m/z, 441).The molecular weight difference of 449/475, 431/457, and 415/441 were the same as the 433/459.Therefore, the aglycone structures of 449, 431, and 415 in PQ-ginsenoside B, C, and D were deduced to be degraded from 475, 457, and 441 in the Rg5, Rg6, and Rg5, characterized as 25 For the structures of the ginsenosides, the database CAS SciFinder n was search Among them, 10 ginsenosides were identified in the steamed PQ for the first time.aglycone or sugar chains were different compared with the ginsenosides reported viously.There was one new ginsenoside classified as the PPT-type, and it was nam PPT-O-glc-rha/O-rha. PPT-O-glc-rha/O-rha (m/z 975.5534, [M+HCOO] − ) was identified fragment ions of 929, 783, 621, 475, 205, 163, 161, and 145 (Figure 3A).The fragment io 475 is the characteristic aglycone ion of PPT-type ginsenosides.The neutral los 929/783, 783/621, and 621/475 indicated two rhamnose and a glucose in the sugar cha The fragment ion of 205 indicated a sugar chain of O-glucose-rhamnose.

Method Validation
The repeatability and precision of the analytical method were investigated by QC samples.The repeatability was investigated by six QC samples.The QC samples were analyzed continuously.The RSD of each peak was calculated among the six QC samples.The peak number and area were counted within different RSD ranges (0-10%, 10-20%, 20-30%, and >30%).In total, 98.2% of the peaks had an RSD value of less than 20%, while the accumulated peak area accounted for 99.3% of the total area (Figure S1A).For the intra-day precision analysis, six QC samples were analyzed every 4 h, and 98.1% of the peaks had an RSD less than 20%, while the accumulated peak area accounted for 99.3% of the total peak area (Figure S1B).For the inter-day precision analysis, 18 QC samples were analyzed for 3 days.The results show that 96.0% of the peaks had an RSD of less than 20%, while the accumulated peak area accounted for 98.6% of the total peak area (Figure S1C).These results indicated the good stability of the analytical method.

Difference between Steamed and Freeze-Dried Samples
Principal component analysis (PCA) was carried out in PQ samples with different steaming times and temperatures with UV scaling.In Figure 5A, PC1 and PC2 were 0.608 and 0.245.In Figure 5B, PC1 and PC2 were 0.636 and 0.208.The score plots show that there was obvious separation among the freeze-dried samples and steamed samples.
A non-parametric test was then performed, and the ratio was calculated between each of the two groups.p < 0.05 and ratio > 5 were set as the criteria to screen the differential ginsenosides (Tables S1 and S2).There were 51, 55, 58, 63, 67, and 75 differential ginsenosides found between the steamed samples of 2 h, 4 h, 6 h, 8 h, 10 h, and 12 h and freeze-dried samples, respectively.There were 48, 60, 74, and 89 differential ginsenosides found between steamed samples of 100 °C, 110 °C, 120 °C, and 130 °C and freeze-dried

Method Validation
The repeatability and precision of the analytical method were investigated by QC samples.The repeatability was investigated by six QC samples.The QC samples were analyzed continuously.The RSD of each peak was calculated among the six QC samples.The peak number and area were counted within different RSD ranges (0-10%, 10-20%, 20-30%, and >30%).In total, 98.2% of the peaks had an RSD value of less than 20%, while the accumulated peak area accounted for 99.3% of the total area (Figure S1A).For the intra-day precision analysis, six QC samples were analyzed every 4 h, and 98.1% of the peaks had an RSD less than 20%, while the accumulated peak area accounted for 99.3% of the total peak area (Figure S1B).For the inter-day precision analysis, 18 QC samples were analyzed for 3 days.The results show that 96.0% of the peaks had an RSD of less than 20%, while the accumulated peak area accounted for 98.6% of the total peak area (Figure S1C).These results indicated the good stability of the analytical method.

Difference between Steamed and Freeze-Dried Samples
Principal component analysis (PCA) was carried out in PQ samples with different steaming times and temperatures with UV scaling.In Figure 5A, PC1 and PC2 were 0.608 and 0.245.In Figure 5B, PC1 and PC2 were 0.636 and 0.208.The score plots show that there was obvious separation among the freeze-dried samples and steamed samples.
A non-parametric test was then performed, and the ratio was calculated between each of the two groups.p < 0.05 and ratio > 5 were set as the criteria to screen the differential ginsenosides (Tables S1 and S2).There were 51, 55, 58, 63, 67, and 75 differential ginsenosides found between the steamed samples of 2 h, 4 h, 6 h, 8 h, 10 h, and 12 h and freeze-dried samples, respectively.There were 48, 60, 74, and 89 differential ginsenosides found between steamed samples of 100 • C, 110 • C, 120 • C, and 130 • C and freeze-dried samples, respectively.This indicated that the steam temperature and time had a significant influence on the steamed PQ samples.samples, respectively.This indicated that the steam temperature and time had a significant influence on the steamed PQ samples.

Influence of Steam Temperature and Time on the Ginsenosides Composition
The hierarchical cluster analysis was carried out on the differential ginsenosides.The data were normalized, and the heat map is shown in Figures 6 and S2.There were 104 and 86 differential ginsenosides screened for PQ samples with different steaming temperatures and times, respectively.With the increase in the steaming temperature, the content of 37 ginsenosides increased gradually, while the content of 36 ginsenosides decreased gradually.With the increase in the steaming time, the content of 42 ginsenosides increased gradually, while the content of 15 ginsenosides decreased gradually.In addition, there were some ginsenosides that changed with the steaming time and temperature, for example, climbing up and then declining.The steaming process not only changed the ginsenosides content, but also influenced their structure.

Influence of Steam Temperature and Time on the Ginsenosides Composition
The hierarchical cluster analysis was carried out on the differential ginsenosides.The data were normalized, and the heat map is shown in Figures 6 and S2.There were 104 and 86 differential ginsenosides screened for PQ samples with different steaming temperatures and times, respectively.With the increase in the steaming temperature, the content of 37 ginsenosides increased gradually, while the content of 36 ginsenosides decreased gradually.With the increase in the steaming time, the content of 42 ginsenosides increased gradually, while the content of 15 ginsenosides decreased gradually.In addition, there were some ginsenosides that changed with the steaming time and temperature, for example, climbing up and then declining.The steaming process not only changed the ginsenosides content, but also influenced their structure.
For the PPD-ginsenosides, the difference was shown in Figures 6A and S2A.Firstly, the malonyl-ginsenosides Rb1, Rb2, Rb3, Rd, and Re decreased significantly with the steaming time and temperature.The rate among different samples was calculated.The content of malonyl-ginsenoside Rb3 in the freeze-dried sample was 2-, 323-, and 574-fold more than in the samples (100 • C, 2 h), (100 • C, 12 h), and (130 • C, 2 h), respectively.Malonyl-ginsenoside Rg3 was accumulated after the steaming process, and then decreased with the increase in the steaming time and temperature.Malonyl-ginsenoside Rg3 was increased by 5-fold in the sample (100 • C, 2 h), and then decreased by 26-and 47-fold in the samples (100 • C, 12 h) and (130 • C, 2 h), respectively.Secondly, the acetyl-ginsenosides showed a significant increase after the steaming process.Then, the acetyl-ginsenosides Rc, Rb3, Rb1, and Rd changed slightly with the steaming time, but showed an obviously decline with the increase in the steaming temperature.Acetyl-ginsenoside Rg3 was enhanced from steaming for 8 h at 120 • C. Thirdly, the levels of ginsenosides Rh2, Rg3, and Rb3 and gypenoside XIII were enhanced with the steaming process, while PPD-O-glc-glc/O-glc-rha and saponin Ia were decreased.And, the steaming time was not significant with the ratios.The content of 20(R)ginsenoside Rh2 was extremely low in the freeze-dried sample, and then increased quickly after the steaming process.Although the content of 20(S)-ginsenoside Rh2 was also rising, the increase rate was low.The contents of 20(R)-ginsenoside Rh2 and 20(S)-ginsenoside Rh2 in the sample (100 • C, 2 h) were 22-and 4-fold more than in the freeze-dried sample, indicating the transformation from the S-configuration to the R-configuration.The contents of 20(R)-ginsenoside Rh2 in the samples (100 • C, 12 h) and (130 • C, 2 h) were 8 and 18 times more than in the sample (100 • C, 2 h), respectively.This shows that a high temperature enhances configuration transformation.The malonyl ginsenosides were sensitive to the heat process.The acetyl-ginsenoside could be produced by the decarboxylation of the malonyl group [34].Therefore, with the decrease in the malonyl ginsenosides, the level of acetyl ginsenosides was enhanced.With the rise in temperature, acetyl ginsenosides were then degraded into neutral ginsenosides.The ginsenoside Rg3 could be produced from acetyl-ginsenoside Rg3, and could be further converted into ginsenoside Rh2 through the elimination of glucose at C-3 [35].The acetyl-ginsenoside Rg3 could be produced from malonyl-ginsenoside Rg3.The possible transformation pathways are shown in Figure S3.For the PPD-ginsenosides, the difference was shown in Figures 6A and S2A.Firstly, the malonyl-ginsenosides Rb1, Rb2, Rb3, Rd, and Re decreased significantly with the steaming time and temperature.The rate among different samples was calculated.The content of malonyl-ginsenoside Rb3 in the freeze-dried sample was 2-, 323-, and 574-fold more than in the samples (100 °C, 2 h), (100 °C, 12 h), and (130 °C, 2 h), respectively.Malonyl-ginsenoside Rg3 was accumulated after the steaming process, and then decreased with the increase in the steaming time and temperature.Malonyl-ginsenoside Rg3 was increased by 5-fold in the sample (100 °C, 2 h), and then decreased by 26-and 47-fold in the samples (100 °C, 12 h) and (130 °C, 2 h), respectively.Secondly, the acetyl-ginsenosides showed a significant increase after the steaming process.Then, the acetyl-ginsenosides Rc, Rb3, Rb1, and Rd changed slightly with the steaming time, but showed an obviously decline with the increase in the steaming temperature.Acetyl-ginsenoside Rg3 was enhanced from steaming for 8 h at 120 °C.Thirdly, the levels of ginsenosides Rh2, Rg3, and Rb3 and gypenoside XIII were enhanced with the steaming process, while PPD-O-glc-glc/O-glc-rha and saponin Ia were decreased.And, the steaming time was not significant with the ratios.The content of 20(R)-ginsenoside Rh2 was extremely low in the freeze-dried sample, and then increased quickly after the steaming For the OA-type ginsenosides, chikusetsusaponin Iva and isomer I had the same sugar chains (a glucose and a glucuronic acid attached to different positions of the aglycone).They were decreased after the steaming process (Figures 6C and S2C).The content of chikusetsusaponin Iva and isomer I in the freeze-dried sample were 10 and 14 times higher than in the sample (100 • C, 2 h), respectively.Zingibroside R1 was different at the sugar chains in the structure compared with chikusetsusaponin Iva and isomer I.It had a disaccharide chain of glucose and glucuronic acid.OA-O-glc had glucose in the sugar chain.The contents of OA-O-glc and zingibroside R1 were increased after the steaming process.The contents of OA-O-glc and zingibroside R1 in the sample (100 • C, 2 h) were 12 and 6 times more than in the freeze-dried sample.The sugar chain of ginsenoside Ro has been reported to experience degradation to form the zingibroside R1 and chikusetsusaponin Iva [27].Moreover, it has been deduced that the sugar chain of chikusetsusaponin Iva experiences degradation at C-3 to form OA-O-glc.With the increase in the steaming time and temperature, the content of them were also increased.From 2 h to 10 h, the contents of them increased continuously.At the 12 h, their content showed a little decline.The contents of chikusetsusaponin Iva, chikusetsusaponin Iva isomer I, zingibroside R1, and OA-O-glc in the sample (100 • C, 10 h) were 4, 3, 4, and 5 times more than in the sample (100 • C, 2 h), respectively.From 100 • C to 130 • C, the contents of them increased continuously.The contents of chikusetsusaponin Iva, chikusetsusaponin Iva isomer I, zingibroside R1, and OA-O-glc in the sample (130 • C, 2 h) were 9, 12, 4, and 29 times more than in the sample (100 • C, 2 h), respectively.The steaming temperature had a larger impact on the OA-type ginsenosides' conversion than the steaming time.The possible transformation pathways are shown in Figure S5A.
OT-type ginsenosides are characteristic ingredients of PQ which different from other Panax species [27].The differences are shown in Figures 6D and S2D.In Figure 6D, Pseudoginsenoside F11 isomer III, malonyl-pseudoginsenoside F11, and OT-O-glc-rha/Oxyl/ara show a declining tendency, and pseudoginsenoside Rt4, Rt2, Ft2, and 24(R)pseudoginsenoside F11 show a rising tendency.The contents of malonyl-pseudoginsenoside F11 and OT-O-glc-rha/O-xyl/ara in the sample (100 • C, 2 h) were 69 and 28 times more than in the sample (130 • C, 2 h).The content of malonyl-pseudoginsenoside F11 in the sample (100 • C, 2 h) was 27 times more than in the sample (100 • C, 12 h).The malonylpseudoginsenoside F11 and OT-O-glc-rha/O-xyl/ara underwent demalonylation and hydrolysis reactions to produce the pseudoginsenoside F11, respectively.Pseudoginsenoside Rt4 increased with the time and temperature.The contents of pseudoginsenoside Rt4 in the sample (100 • C, 12 h) and (130 • C, 2 h) was 5 and 10 times more than in the sample (100 • C, 2 h), respectively.The content of 24(R)-pseudoginsenoside F11 in the sample (130 • C, 2 h) was six times more than in the freeze-dried sample.The content of pseudoginsenoside Rt2 was the highest at 110 • C, and then declined.The sugar chain of 24(R)-pseudoginsenoside F11 (the sugar chain of glucose-rhamnose) and pseudoginsenoside Rt2 (the sugar chain of a glucose-xylose) underwent degradation to form the pseudoginsenoside Rt4 (the sugar chain of a glucose).These results indicate the degradation of the sugar chains with the steaming process, and the high temperature facilitated the degradation process.The possible transformation pathways are shown in Figure S5B.
In Figures 6E and S2E, most other-type ginsenosides increase with the steaming process.Firstly, the steaming time and temperature have little difference in their influence on ginsenoside Rg5, floralginsenoside Tb, ginsenoside Rg6, ginsenoside Rg5 isomer II, and ginsenoside Rg6 isomer II.The contents of ginsenoside Rg5 in the samples (100 • C, 12 h) and (130 • C, 2 h) were five and four times more than in the sample (100 • C, 2 h), respectively.The contents of ginsenoside Rg6 in the samples (100 • C, 12 h) and (130 • C, 2 h) was four-and three-fold higher than in the sample (100 • C, 2 h), respectively.Secondly, the effect of the steaming temperature on the contents of ginsenoside Rs4, ginsenoside Rh4, ginsenoside Rg6 isomer I, 20(R)-ginsenoside Rh12, PQ-ginsenoside A, C, D, and acetyl-and the drying gas temperature was 200 • C. The prepulse storage was 8 µs, the collision RF was 750 Vpp, and the transfer time was 80 µs.The collision energies were set at 40-70 eV.

Data Analysis
MS-DIAL software (version 4.9.0)wasused for date deconvolution and peak alignment.A dataset containing m/z, retention time and peak area was obtained.Principal component analysis (PCA) was performed using Simca 14.0 (Umetrics, Umeå, Sweden).The hierarchical cluster analysis and non-parametric test were performed using MultiExperiment Viewer software (version 4.9, Dana-Farber Cancer Institute, Boston, MA, USA).The select mode for non-parametric test was Wilcoxon and Mann-Whitney test (one factor, two experimental groups).

Conclusions
A comprehensive investigation was carried out on the ginsenoside identification and transformation of PQ samples with different steaming conditions.In total, 175 ginsenosides were identified, and the sugar chains were annotated based on UHPLC-QTOF-MS.New ginsenosides and isomers were discovered.The steaming process was an effective method to increase the chemical diversity of the ginsenosides.The types and contents of the ginsenosides were found to vary greatly.The content of acylated ginsenosides and protoginsenosides decreased, while the content of the rarest ginsenosides significantly increased after the steaming process.This study can deepen the understanding of the ginsenosides' conversion in PQ during the steaming process.Since the definite functions of the individual ginsenoside have not been revealed clearly, further research is needed.

Supplementary Materials:
The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules29030623/s1, Figure S1: The peak number and accumulated peak area with different RSD ranges for the repeatability (A), intra-day (B), and inter-day (C) precision.S1: The p and ratio of differential ginsenosides in the samples with different steaming times.Table S2: The p and ratio of differential ginsenosides in the samples with different steaming temperatures.

Figure 1 .
Figure 1.Total ion chromatogram of steamed PQ with LC-MS in the negative ion mode.

Figure 1 .
Figure 1.Total ion chromatogram of steamed PQ with LC-MS in the negative ion mode.

Figure 1 .
Figure 1.Total ion chromatogram of steamed PQ with LC-MS in the negative ion mode.

Figure 5 .
Figure 5. PCA score plots of ginsenosides in PQ samples with different steaming temperatures (A) and times (B).

Figure 5 .
Figure 5. PCA score plots of ginsenosides in PQ samples with different steaming temperatures (A) and times (B).

Funding:
The work was funded by the National Natural Science Foundation of China (21904080), the New Innovation Team of Jinan (202228020), the Shandong Province Taishan Scholar Program (tstp20221138), the Key Research and Development Program of the Shandong Province (2022TZXD0034), and the Science, Education, and Industry Integration Innovation Pilot Project from Qilu University of Technology (Shandong Academy of Sciences) (2023PY048).

.
Ginsenosides identified in the steamed PQ samples.

Table 1 .
Ginsenosides identified in the steamed PQ samples.

Table 1 .
Ginsenosides identified in the steamed PQ samples.