CD36: The Bridge between Lipids and Tumors

It has been found that the development of some cancers can be attributed to obesity, which is associated with the excessive intake of lipids. Cancer cells undergo metabolic reprogramming, shifting from utilizing glucose to fatty acids (FAs) for energy. CD36, a lipid transporter, is highly expressed in certain kinds of cancer cells. High expressions of CD36 in tumor cells triggers FA uptake and lipid accumulation, promoting rapid tumor growth and initiating metastasis. Meanwhile, immune cells in the tumor microenvironment overexpress CD36 and undergo metabolic reprogramming. CD36-mediated FA uptake leads to lipid accumulation and has immunosuppressive effects. This paper reviews the types of FAs associated with cancer, high expressions of CD36 that promote cancer development and progression, effects of CD36 on different immune cells in the tumor microenvironment, and the current status of CD36 as a therapeutic target for the treatment of tumors with high CD36 expression.


Introduction
Lipids are among the most important nutrients required by the human body.They consist of many different types of molecules, including phospholipids, fatty acids (FAs), triglycerides, sphingolipids, cholesterol, and cholesteryl esters [1].While the majority of human FAs are obtained from food, the body can also convert sugars and proteins into FAs, a process that takes place primarily in the liver [2].Moreover, FAs are the primary energy source of cells.During periods of low energy demand, the adipose tissue can store excess FAs as triacylglycerols (TAG) in intracellular lipid droplets (LDs).Such reserves are used as buffers to maintain cellular lipid composition homeostasis or as a source of energy to drive cellular processes [3].Therefore, certain amounts of lipids must be consumed daily.However, in some cases, excessive intake may lead to the development of a variety of diseases.
Obesity is a severe public health problem and is typically associated with diet.Obesity increases the risk of metabolic diseases and can also lead to premature deaths [4].It is found that about 25% of adults in industrialized countries are clinically diagnosed as obese and 60% as overweight, and this situation is likely to worsen in the future.Moreover, excess body fat can increase the risk of developing different kinds of cancer, such as esophageal, colorectal, gallbladder, pancreatic, endometrial, and breast cancers, with respect to postmenopausal women, and advanced prostate cancer, with respect to men.Evidence has shown that 4-38% of these cancers, depending on the location of the tumor and the patients' sex, can be attributed to overweight/obesity [5].Excessive food intake, especially a high-fat diet, is the most critical factor in the development of obesity [6].Meanwhile, a high-fat diet can cause many types of cancer, such as ovarian cancer [7], prostate [8], skin [9], breast [10], pancreatic [11], and liver cancers [12].
The Western dietary pattern is considered a typically high-fat diet, characterized by a high intake of refined starches, sugar, red meat, processed red meat, saturated and trans fats, Table 1.Cancer-related foods and correlative cancer types.(Cancer types in the table have been ordered according to the correlation between food and cancer risk in the results of the literature).

Fatty Acids in the Diet and Their Relationship to Cancer
Tumor cells can acquire lipids by both endogenous synthesis and external uptake.The following are fatty acids that can be consumed in diets and their relationship to cancer development.

Saturated Fatty Acids (SFAs)
Saturated fatty acids are a class of fatty acids that do not have an unsaturated bond in the carbon chain and are one of the essential components of lipids.SFAs come mainly from red meats and dairy products [45].The current consensus among nutritionists is that saturated fats can be consumed in moderation with a balanced diet.There are even some controversial results about SFAs and the risk of cancer.However, most reports point to a diet heavy in saturated fat as a potential tumor promoter.Findings from a metaanalysis indicate that consuming more SFAs in the diet increased the risk of liver cancer [12].Another study revealed that SFAs from red meats and processed meats were positively related to oral cancer risk [45].A high intake of SFAs from red meats was associated with an increased risk of lung cancer [46].Another meta-analysis found that a higher dietary intake of SFAs was associated with a higher risk of breast cancer after menopause [47].SFAs and cholesterol synergistically promoted prostate cancer stem cell proliferation and contributed to prostate cancer progression [8].Palmitic acid (PA) is the most common SFA in the body, and people can obtain PA through their diets.Pancreatic cancer risk was strongly associated with the intake of PA [48].In addition, studies have suggested that such diets may reduce the effectiveness of anticancer treatments [49].PA enters gastric cancer (GC) cells through its receptor CD36, promoting GC metastasis [50].A palmitic acid-rich diet fed to mice advanced colon cancer tumor growth [51].Therefore, an excessive intake of SFAs can increase the risk of cancer and poor prognosis.

Unsaturated Fatty Acids
Unsaturated fatty acids can be categorized according to the number of double bonds in monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), where monounsaturated fatty acids contain only one double bond in the carbon chain.In contrast, polyunsaturated fatty acids have two or more double bonds.These two types of unsaturated fatty acids act in diametrically opposed ways.
MUFAs: Among MUFAs, oleic acid (OA) is the human diet's most abundant representative fatty acid.OA induced Hela cell growth and metastasis by promoting high CD36 expression [52].It could also enhance the proliferation of breast cancer (MCF7) cells [53].Moreover, it enabled the invasiveness of gastric cancer cells [54].OA promoted the invasiveness of the prostate cancer cell line PC3 [55].However, with a 78% oleic acid content, olive oil contains many bioactive substances, such as polyphenols, with antitumor and anti-inflammatory properties, and its consumption is considered an essential factor in maintaining a healthy lifestyle [56].Olive oil is the primary source of unsaturated fatty acids in the Mediterranean diet.Many nuts contain mainly MUFAs (mainly OA), and nuts, as an alternative source of MUFAs, may reduce cancer risk [49].These conclusions encourage people to consume fat from plants.
PUFAs: ω-6 PUFAs and ω-3 PUFAs 22 are two main types of fatty acids [49].Linoleic acid (LA), an ω-6 polyunsaturated fatty acid, can most often be found in dietary fats and most oils, except flaxseed oil, which contains the highest amount of Alpha-linolenic acid (ALA), the most common ω-3 polyunsaturated fatty acid.PUFAs can also be found in soybean oil, canola oil, and fish [49].As essential FAs, LA and ALA can lead to several balance-maintaining products in our bodies, such as arachidonic acid (AA, ω-6), eicosapentaenoic acid (EPA, ω-3), and docosahexaenoic acid (DHA, ω-3) [57].People come to obtain DHA and EPA mainly through their diet (mainly fish oil) [58].Feeding mice a diet high in omega-6 fatty acids (containing 23% corn oil) promotes pancreatic tumorigenesis in vivo [59].CT26 mouse colon cancer cells were inoculated on the backs of BALB/c mice, and LA was administered by gavage.The results showed that LA promoted tumor cell growth and induced cancer cell stemness, which in turn promoted tumor metastasis [60].Nude mice transplanted with human gastric cancer cells (OCUM-2MD3) and then fed LA showed that LA could stimulate gastric cancer cell invasion and peritoneal metastasis by cyclooxygenase (COX)-catalyzed metabolism and ERK activation [61].In vitro, LA induced the migration and invasion of breast cancer cells via the FFAR4, EGFR, and PI3K-/Akt pathways [62].Roberto Espinosa-Neira et al. studied LA in MCF10A human mammary epithelial cells and, for the first time in 2011, substantiated that LA could promote an epithelial-mesenchymal transition (EMT)-like process in those cells through activating FAK and NFκB, affecting MMP-2 secretions, inducing cell migration and cancer invasion, and regulating the expression levels of many proteins, such as decreasing E-cadherin expression while increasing Snail1, Snail2, Twist1, Twist2, and Sip1 expressions [63].There was a positive association among US adults between hepatocellular carcinogenesis (HCC) and the total intake of PUFAs and ω-6 PUFAs.In contrast, the intake of long-chain omega-3 fatty acids and MUFAs was negatively associated with liver cancer [64].ω-6 PUFAs could enhance the metastatic ability of gastric cancer cells through the COX-2/PGE2 signaling pathway, whereas ω-3 PUFAs serve to inhibit that ability through the COX-1/PGE3 signaling axis [65].In breast cancer cells, ω-3 PUFAs induced the downregulation of EZH2, thus exerting anticancer effects [66].In an induced hamster oral cancer model, it was found that low tumor numbers and volumes were associated with low intakes of ω-6/ω-3 fatty acid.Researchers also found that such a decrease in tumor burden in the right buccal pouch tissue also showed a connection with lower levels of NFκB, proliferating cell nuclear antigen and cyclin D1 [67].A recent analysis suggested that increasing the dietary intake ratio of ω-3/ω-6 PUFAs could benefit breast cancer prevention [68].Adjusting the balance of dietary ω-3/ω-6 PUFAs might help prevent some cancers.

Trans Fatty Acids (TFAs)
TFAs are MUFAs or PUFAs with one or more double bonds in the trans configuration.Most TFAs derive from hydrogenated fats; only a small part comes from animal oils.With the development of the food industry, TFAs have been widely found in diets such as margarine, cookies, and repeated cooking oils.A high intake of TFAs puts prostate and colorectal cancer (CRC) at higher risk [69].Industrially produced TFAs (iTFAs) are the primary source of TFAs in the human diet.Early studies on the health risks of TFAs in humans focused on the cardiovascular aspects related to iTFAs [70].Elaidic acid (EA) is the most representative iTFA.The results of a recent analysis suggest that higher iTFA intake, especially EA, is associated with an increased risk of breast cancer [71].A piece of evidence indicated that a higher intake of industrial trans-EA is associated with a higher risk of ovarian cancer [72].An in vivo study pointed out that EA could promote stemness markers in CT26 cells, a mouse CRC cell, thereby increasing their metastasis ability [73].A study related to NHL found a positive association between TFA intake and the risk of diffuse large B-cell lymphoma (an aggressive NHL subtype) [74].Another study using hepatitis C virus (HCV) core gene transgenic mice added that a high TFA intake could also lead to tumors in livers, and that multiple signaling pathways were involved, such as NF-κB and NRF2-p62/SQSTM1 signaling, ERK and Wnt/β-catenin pathways [75].The WHO recommends that the total trans fatty intake should be limited to less than 1% of the total energy intake, which translates to less than 2.2 g/day, based on a 2000-calorie diet.
An overview of the relationships among SFAs, MUFAs, PUFAs, and TFAs with cancer is shown in Figure 1.Above all, the intake of fatty acids has an essential impact on tumor occurrence, invasion, and metastasis.Therefore, a moderate intake of fatty acids is of great significance for preventing and improving cancer prognosis.

Metabolic Reprogramming of Tumor Cells
Malignant tumors pose a severe threat to human health.Tumor cells reprogram their metabolic phenotype to meet increased bioenergetic demands and support their malignant behaviors in a nutrient-poor environment, causing their metabolic pattern to be different from that of normal cells.Normal cells derive energy through two main processes: oxidative phosphorylation and glycolysis.Oxidative phosphorylation occurs in the mitochondria and relies on the presence of adequate oxygen.It efficiently converts glucose into ATP, the cellular energy currency.In contrast, glycolysis takes place in the cytoplasm and operates when oxygen availability is insufficient.It breaks down glucose into pyruvate, producing a smaller amount of ATP.These two mechanisms allow normal cells to adapt to varying oxygen levels and generate energy efficiently.The "Warburg effect" defines the glucose metabolism of tumor cells, wherein, regardless of oxygen levels, tumor cells utilize glycolysis to convert pyruvate from glucose into lactate, thereby generating ATP.Hence, tumor metabolism is often referred to as "aerobic glycolysis".More importantly, cancer cells deplete nutrients and produce immunosuppressive metabolites that interfere with immune cell function [76].Along with aerobic glycolysis, tumor cells use glutamine, serine, arginine, fatty acids, and lipids to promote their proliferation.Among them, fatty acids are one of the most vital energy sources [77].The proliferation of cancer cells necessitates the accumulation of substantial lipid quantities, which can be acquired from external sources, or synthesized internally through the adipogenic pathway.Clinical investigations are presently underway to explore the potential of fatty acid synthase inhibitor drugs targeting the fatty acid synthesis pathway.Among these inhibitors, TVB-2640, a small molecule compound, has exhibited inhibitory effects on fatty acid synthase in clinical studies [3].The reprogramming of fatty acid metabolism is a feature of malignant tumors.Fatty acid uptake, storage, and lipogenesis are upregulated in various cancers, and tumor cells obtain energy through FA oxidation (FAO), which helps promote rapid tumor growth.Specific metabolic activities can participate directly in the transformation processes or support the biological processes that enable tumor growth [78].
At different stages of cancer development, lipid metabolism shows a generalized enhancement that provides a specific energy source for tumor cells, triggers particular signaling pathways and epigenetic events, and remodels membrane components that favor metastasis [79].Metastatic tumor cells possess the ability to undergo EMT, which leads to

Metabolic Reprogramming of Tumor Cells
Malignant tumors pose a severe threat to human health.Tumor cells reprogram their metabolic phenotype to meet increased bioenergetic demands and support their malignant behaviors in a nutrient-poor environment, causing their metabolic pattern to be different from that of normal cells.Normal cells derive energy through two main processes: oxidative phosphorylation and glycolysis.Oxidative phosphorylation occurs in the mitochondria and relies on the presence of adequate oxygen.It efficiently converts glucose into ATP, the cellular energy currency.In contrast, glycolysis takes place in the cytoplasm and operates when oxygen availability is insufficient.It breaks down glucose into pyruvate, producing a smaller amount of ATP.These two mechanisms allow normal cells to adapt to varying oxygen levels and generate energy efficiently.The "Warburg effect" defines the glucose metabolism of tumor cells, wherein, regardless of oxygen levels, tumor cells utilize glycolysis to convert pyruvate from glucose into lactate, thereby generating ATP.Hence, tumor metabolism is often referred to as "aerobic glycolysis".More importantly, cancer cells deplete nutrients and produce immunosuppressive metabolites that interfere with immune cell function [76].Along with aerobic glycolysis, tumor cells use glutamine, serine, arginine, fatty acids, and lipids to promote their proliferation.Among them, fatty acids are one of the most vital energy sources [77].The proliferation of cancer cells necessitates the accumulation of substantial lipid quantities, which can be acquired from external sources, or synthesized internally through the adipogenic pathway.Clinical investigations are presently underway to explore the potential of fatty acid synthase inhibitor drugs targeting the fatty acid synthesis pathway.Among these inhibitors, TVB-2640, a small molecule compound, has exhibited inhibitory effects on fatty acid synthase in clinical studies [3].The reprogramming of fatty acid metabolism is a feature of malignant tumors.Fatty acid uptake, storage, and lipogenesis are upregulated in various cancers, and tumor cells obtain energy through FA oxidation (FAO), which helps promote rapid tumor growth.Specific metabolic activities can participate directly in the transformation processes or support the biological processes that enable tumor growth [78].
At different stages of cancer development, lipid metabolism shows a generalized enhancement that provides a specific energy source for tumor cells, triggers particular signaling pathways and epigenetic events, and remodels membrane components that favor metastasis [79].Metastatic tumor cells possess the ability to undergo EMT, which leads to their disparities from normal tumor cells.In this process, metastatic tumor cells obtain skills related to invasion, metastasis, and the resistance to radiotherapy and chemother-apy [80].During tumor progression and metastasis, fatty acids provide sufficient energy to tumor cells [81].One study found that sentinel lymph node metastases showed a greater degree of fatty acid accumulation when compared to the primary tumor [82].Tumor cells optimize their requirements for aggressive progression by switching lipid anabolism and catabolism.Fatty acids are a component of most lipids and have been shown to drive tumor progression [83].The uptake of exogenous fatty acids into cancer cells could facilitate metastasis [84].Fatty acid uptake is critical in fatty acid transportation during cell membrane biosynthesis, energy storage, and signaling pathway activation [85].A transport system that assisted fatty acid translocation involved CD36 and FABPs, working as transporters that collected fatty acids from the surrounding environment and then moved them across the plasma membrane, thus pointing out their crucial position in reorganizing the metabolic phenotype of tumor cells.Recent studies have shown that CD36 expression promoted the metastasis of human oral carcinoma cells, ovarian cancer cells, gastric cancer cells, pancreatic cancer cells, and breast cancer cells [81, 86,87].Andras et al. found that, except for CD36, the most prevalent FA transporters (such as FABPpm, FATP1, and FATP4) were unaffected by human primary adipocyte coculture [87].Human primary adipocyte coculture increased CD36 protein levels in tumor cells; furthermore, tumor cells that express high levels of the fatty acid receptor CD36 and lipid metabolism genes are unique in their ability to initiate metastasis [44].Recent studies have revealed a notable disparity in the expression of CD36 between metastatic and non-metastatic cancer cells, indicating a higher level of CD36 in the former [88].It seems that CD36 expression affects the metabolism of tumor cells by reducing glucose oxidation and promoting a higher uptake and storage of lipids from the diet, rather than activating the synthesis of in-house lipids.Palmitic acid, or a high-fat diet, explicitly boosts the metastatic potential of CD36 + metastasis-initiating cells in a CD36-dependent manner [44].The metabolic characteristics of normal cells, tumor cells, and metastatic-initiating cells are shown in Figure 2.
Molecules 2024, 29, x FOR PEER REVIEW 6 of 21 their disparities from normal tumor cells.In this process, metastatic tumor cells obtain skills related to invasion, metastasis, and the resistance to radiotherapy and chemotherapy [80].During tumor progression and metastasis, fatty acids provide sufficient energy to tumor cells [81].One study found that sentinel lymph node metastases showed a greater degree of fatty acid accumulation when compared to the primary tumor [82].Tumor cells optimize their requirements for aggressive progression by switching lipid anabolism and catabolism.Fatty acids are a component of most lipids and have been shown to drive tumor progression [83].The uptake of exogenous fatty acids into cancer cells could facilitate metastasis [84].Fatty acid uptake is critical in fatty acid transportation during cell membrane biosynthesis, energy storage, and signaling pathway activation [85].A transport system that assisted fatty acid translocation involved CD36 and FABPs, working as transporters that collected fatty acids from the surrounding environment and then moved them across the plasma membrane, thus pointing out their crucial position in reorganizing the metabolic phenotype of tumor cells.Recent studies have shown that CD36 expression promoted the metastasis of human oral carcinoma cells, ovarian cancer cells, gastric cancer cells, pancreatic cancer cells, and breast cancer cells [81, 86,87].Andras et al. found that, except for CD36, the most prevalent FA transporters (such as FABPpm, FATP1, and FATP4) were unaffected by human primary adipocyte coculture [87].Human primary adipocyte coculture increased CD36 protein levels in tumor cells; furthermore, tumor cells that express high levels of the fatty acid receptor CD36 and lipid metabolism genes are unique in their ability to initiate metastasis [44].Recent studies have revealed a notable disparity in the expression of CD36 between metastatic and non-metastatic cancer cells, indicating a higher level of CD36 in the former [88].It seems that CD36 expression affects the metabolism of tumor cells by reducing glucose oxidation and promoting a higher uptake and storage of lipids from the diet, rather than activating the synthesis of in-house lipids.Palmitic acid, or a high-fat diet, explicitly boosts the metastatic potential of CD36 + metastasis-initiating cells in a CD36-dependent manner [44].The metabolic characteristics of normal cells, tumor cells, and metastatic-initiating cells are shown in Figure 2. In addition to using glucose in glycolysis for energy, tumor cells take up free fatty acids via CD36 and utilize fatty acid functions to meet the demands of high proliferation.Metastatic tumor cells demonstrate an augmented metabolic activity, particularly in glucose and fatty acid metabolism, when compared to primary tumors.This metabolic adaptation enables metastatic tumor cells to In addition to using glucose in glycolysis for energy, tumor cells take up free fatty acids via CD36 and utilize fatty acid functions to meet the demands of high proliferation.Metastatic tumor cells demonstrate an augmented metabolic activity, particularly in glucose and fatty acid metabolism, when compared to primary tumors.This metabolic adaptation enables metastatic tumor cells to enhance fatty acid uptake and metabolism, thereby utilizing fatty acids as an additional energy source to satisfy their elevated energy requirements.

The Functions of CD36
CD36, an extensively glycosylated 80 kDa integral membrane protein, is expressed in a variety of cell types, including platelets, monocyte phagocytes, adipocytes, hepatocytes, myocytes, and certain epithelial cells.CD36 is sometimes called GPIV, GPIIIb, PAS IV, or FAT [43].CD36 belongs to the scavenger receptor B class 2 (SR-B2) and is a transmembrane glycoprotein with two short intracellular domains, two transmembrane segments, and a large extracellular region [89].Its intracellular C-terminus can bind to tyrosine kinase and initiate CD36-mediated signaling [43].CD36 binds and internalizes long-chain fatty acids (LCFAs), and oxidizes low-density lipoprotein (oxLDL), thrombospondin-1, and pathogenassociated molecules.Its extracellular structural domain can bind to CD36-related ligands, such as lipid-associated ligands.LCFAs enter cells and serve as a source of energy, with a carboxyl group and a methyl group present at each end of their molecular structure.As Figure 3 shows, CD36 mediates the uptake of cellular LCFAs in three steps.The first step is extracellular uptake, where CD36 serves as a fatty acid receptor that binds the corresponding LCFAs and connects them to specific lipid raft structures on the plasma membrane.The second step is translocation, where the polar carboxyl groups of fatty acids flip from extracellular to intracellular through the lipid bilayer and relocate.The third step is desorption, where fatty acids enter the cytoplasm from the internal binding site of the plasma membrane and bind specifically to fatty acid-binding proteins (FABPs) [90].FABPs are a group of intracellular proteins that have the ability to bind and transport fatty acids within cells.These proteins play a crucial role in cellular metabolism by binding to free fatty acids and facilitating their transport to specific organelles or enzymes for further breakdown and utilization.Through their binding and transport functions, FABPs help to regulate the metabolism of fatty acids and ensure their efficient utilization within the cell.

High CD36 Expression Promotes Cancer Progression
CD36 is highly expressed in many types of cancer cells and also promotes cancer progression.Pascual reported that a subpopulation of CD44+ cells with a high expression of CD36 and lipid metabolism genes has the ability to initiate metastasis in human OSCC [44].Another piece of evidence showed that CD36 could promote proliferation and mi- As a central regulator of lipid accumulation, CD36 was first associated with the formation of atherosclerosis (AS).In this chronic inflammatory disease, lipids in the blood are deposited in the intima of arteries, causing the fibrous thickening of the intima, necrosis, and the disintegration of deep tissue in order to form atheromatous material that hardens the arterial wall and narrows the lumen [91].CD36 is involved in this process by augmenting lipid accumulation and inflammatory vesicle activation [92].OxLDL, a key molecule [93], binds to CD36 and stimulates macrophages to take themselves up.Then, macrophages transform into foam cells, producing large amounts of inflammatory factors and initiating a series of inflammatory responses, thus driving the development of AS.
Recently, the relationship between CD36 and tumor progression and metastasis has been a concern.CD36 is expressed in tumor cells, microvascular endothelial cells, stromal cells, and immune cells in tumor tissues.However, CD36 levels vary among different cell types [94].CD36 expression is significantly upregulated in malignant epidermal tumors, such as ovarian cancer, gastric cancer, glioblastoma (GBM), and oral squamous cell carcinoma (OSCC).

High CD36 Expression Promotes Cancer Progression
CD36 is highly expressed in many types of cancer cells and also promotes cancer progression.Pascual reported that a subpopulation of CD44+ cells with a high expression of CD36 and lipid metabolism genes has the ability to initiate metastasis in human OSCC [44].Another piece of evidence showed that CD36 could promote proliferation and migration in breast cancer cells, pointing out their pro-tumorigenic role in breast cancer [95].The upregulation of CD36 expression promoted CRC metastasis through the upregulation of MMP28, and increased E-cadherin cleavage [96].CD36 is highly expressed in GC cells, and its levels positively correlate with migration, invasion, and EMT marker expression in GC cell lines [97].Of the causes of GC cancer recurrence and metastasis, peritoneal metastasis (PM) is the most common [98].CD36 expression was upregulated in gastric cancer cells under hypoxic conditions, and the upregulation of CD36 expression promotes the migratory and invasive ability of GC cells and peritoneal tumor growth using exogenous FFA [99].
In vitro experiments showed that CD36 promotes EMT progression in cervical cancer cells by interacting with TGF-β [100].Omental adipocytes reprogram tumor metabolism by upregulating CD36 in OvCa cells, thereby promoting tumor cell invasion and migration [87].CD36 plays a protumor role in glioblastoma cancer stem cells [101].CD36 also assisted in facilitating the proliferation and migration activity of OSCC cells [102].Luo et al. found that CD36 is highly expressed in HCC and promotes the development of HCC through the Src/PI3K/AKT/mTOR signaling pathway [103].Another study found that CD36 induced fatty acid uptake through the regulation of AKR1C2, which in turn affected the development of HCC both in vivo and in vitro [104].

CD36 Improves Drug Resistance in Tumors
Tumor drug resistance is a significant cause of the limited effectiveness of tumor treatment and postoperative recovery.Currently, tumor drug resistance can be categorized into intrinsic and acquired resistance, based on the time of emergence [105].Inherent drug resistance, the natural resistance of tumor cells to a specific antitumor drug, independent of whether they have been exposed to that drug, might result from tumor heterogeneity or the expression of specific mutated oncogenes or tumor suppressor genes in tumor cells that are capable of affecting the expression of drug resistance.Acquired resistance refers to the ability of tumor cells to develop resistance induced by chemotherapeutic agents.Tumor cells might initially be sensitive to a particular chemotherapeutic agent when first encountering it; however, they could develop resistance to the same agent in later treatment, causing tumor relapse.Acquired drug resistance might arise due to the activating of a second proto-oncogene as an emerging driver gene, mutations in the drug target, altered expression levels, or changes in the tumor microenvironment after treatment [105].Multiple studies identified a role for lipid metabolism in chemotherapy resistance in malignant tumors [106][107][108].IL-6 has been reported, by Zhang et al. in a study in 2022, to facilitate chemoresistance in acute myelocytic leukemia (AML) through the uptake of FAs, regulated by stat3/CD36FA uptake [109].After injecting mice with CD36 knockout cells, Zhang et al. also found that the leukemia burden was significantly reduced and, after treating them with cytosine arabinoside (Ara-c), they had a more extended survival time [109].Interestingly, many patients with pancreatic ductal adenocarcinoma (PDAC) are resistant to gemcitabine; CD36 affects gemcitabine resistance by regulating anti-apoptotic proteins, and high CD36 expression in PDAC is critical for poor prognosis [110].Overexpression of HER2 is observed in approximately 20% of breast cancers and is associated with more aggressive tumor progression and unfavorable prognosis [111].Due to the development of drug resistance in tumor cells, current HER2-targeted therapies typically only result in short-term efficacy.CD36 expression is upregulated in lapatinib-resistant cells, facilitating their ability to uptake exogenous fatty acids [112].In vitro and in vivo experimental studies have demonstrated that inhibiting CD36 can suppress the growth of lapatinib-resistant cells [112].

CD36 + Cells Respond to Dietary Lipids and Lead to Metastasis Initiation
Several studies have shown that CD36 is enormously elevated in tumor cells that are cocultured with adipocytes, which means that the expression of CD36 in tumor cells is sensitive to the concentration of fatty acids [44,87].Furthermore, there was a correlation between increased CD36 + cells in oral and metastatic lesions and a high-fat diet in mice.This correlation led to a CD36-dependent mechanism that promoted widespread lymph node metastasis.However, CD36 cells did not generate solitary lymph node metastasis under the same conditions.CD36 + cells are not only uniquely capable of initiating metastasis.Still, they can also recapitulate their molecular and cellular heterogeneity from the primary origin, representing bona fide metastasis-initiating cells [44].
However, in contrast to tumor cells, the expression of CD36 is downregulated in tumor microvessels and stroma [94].For example, high mammographic density (MD) is a significant risk factor associated with an increased incidence of breast cancer, and high MD (but cancer-free) tissue shows reduced levels of CD36 [113].Therefore, CD36 has different effects on tumor cells and cells in the tumor microenvironment (TME).TME, the site of tumorigenesis and growth, is a complex ecosystem of multiple cellular components, including many endothelial cells, fibroblasts, immune cells, and malignant cells [114].The functions of immune cells are critical to tumorigenesis.In the next section, our focus will be on examining the impact of CD36 on immune cells within the TME.

CD36 Suppresses T-Cell Activation
The tumor microenvironment is very detrimental to effector T cells, causing them to undergo apoptosis in tumors [115].However, regulatory T (T reg ) cells are able to survive and perform their suppressive functions in the TME, suggesting that tumor-infiltrating T reg cells may be able to activate metabolic pathways that help them maintain their functions [116].In the TME, T reg cells can accumulate at a high frequency, inhibit effector immune cell function, and promote tumor growth [117].Therefore, targeting T reg cells for anticancer immunotherapy is of great clinical importance.In addition to being crucial to tumor suppression, T reg cells are vital to life because they can maintain immune homeostasis by suppressing immune cell function, thereby preventing spontaneous autoimmunity [118].Therefore, a high-precision target is needed to destroy T reg cells within the tumor, while preserving T reg cells in nontumor tissues [116].Wang et al. reported in 2020 that CD36 expression and lipid metabolism could suppress tumor-infiltrating T reg cells on antitumor CD8 + T-cell responses [119].They also pointed out that increased CD36 expression could promote tumor cell survival and accumulation in the tumor microenvironment [119].Built on the fact that CD36 is only upregulated by T reg cells in tumors, it is safe to infer that CD36 could provide a specific target for the local, rather than systemic, inhibition of T reg cells [119].
The TME is highly immunosuppressive, rendering CD8 + T cells dysfunctional [120].Two studies in 2021 corroborated this statement, showing that CD36 expression could be increased in CD8+ tumor-infiltrating lymphocytes (TILs) in human cancers by the cholesterol that exists in TME, and that such an increase could further increase lipid uptake, accumulation, and peroxidation, leading to CD8 + TIL dysfunction [121,122].Additionally, Ma et al. showed that CD36 expression plays a crucial role in making CD8+ T cells less effective in the anti-tumor process by supplying them with fatty acids, thus leading to a decrease in cytokine production and ferroptosis.Such losses in anti-tumor effects of CD8 + T cells could be reduced by inhibiting ferroptosis and using immune checkpoint inhibitors.They also found a high amount of PUFAs in the TME, which reduced the production of CD8 + effector T-cell cytokines and enhanced the effect of iron ions.Arachidonic acid (AA, ω-6 polyunsaturated fatty acid) was the most often seen PUFA in tumor tissues, which could induce ferroptosis in CD8 + T cells [121].Notably, AA is frequently present in tissues with albumin or the S100A8/S100A9 complex (neutrophil-derived alarmins), which increases the possibility that this complex may mediate supernatural iron degeneration through CD36 signaling [123].Xu et al., in their 2021 study, found that oxidized phospholipids could be a crucial TME lipid, triggering CD36-mediated CD8 + TIL dysfunction [122].They found that CD36 promoted oxLDL entry into T cells and induced lipid peroxidation and the downstream activation of p38 kinase.In vitro experiments have proven that effector T-cell function could be restored by inhibiting p38.The restoration of CD8 + TIL function has been confirmed by in vivo experiments through the resolution of lipid peroxidation via the overexpression of glutathione peroxidase 4.
In conclusion, T cells in the tumor microenvironment are dysfunctional due to the regulation of CD36, which leads to immune suppression and promotes tumor development.

CD36 Plays an Essential Role in the Polarization of Tumor-Associated Macrophages (TAMs) to the M2 Type
Macrophages are essential natural immune cells that are involved in various physiological and pathological activities of the body.Under normal physiological conditions, antigen-presenting cells (mainly including macrophages and dendritic cells) activate downstream antigen-specific T-cell responses through phagocytosis, antigen presentation, and inflammatory responses, which in turn initiate systemic immune responses and form effective immune memory [124].Thus, macrophage-mediated immune surveillance plays a crucial role in the early stages of tumor development.However, mounting evidence suggests that tumor-associated macrophages (TAMs) not only promote tumor progression, but also exert negative effects on cancer therapy as the tumor advances [125,126].Macrophages are highly remodeled and heterogeneous; they are also classified into classically activated M1-type macrophages and, alternatively, activated M2-type macrophages, based on their activation status [127,128].Under the action of multiple factors in the TME, M1-type and M2-type macrophages can interchange.In the early stage of tumors, macrophages are mainly the M1 type and gradually convert to M2 in order to support tumor growth as the cancer progresses.It is generally accepted that the role of TAMs in cancer is similar to that of M2-type macrophages, acting as tumor promoters [129].
TAMs are the most abundant immune cells in the TME, where they play a crucial role in tumorigenesis and development [130].In animal tumor models, TAMs are considered to be critical inducers of the angiogenic switch.TAMs are potent sources of VEGF and many other proangiogenic factors, such as semaphorin and S100A family members, titanaselike proteins, bone-bridging proteins, and secreted proteins that are acidic and rich in cysteine [131].TAMs are closely associated with tumor cell invasion and metastasis.The extracellular matrix (ECM) is a scaffold and barrier for tumor cell migration.TAMs can secrete matrix metalloproteinases (MMPs), serine proteases, and histones in order to mediate ECM degradation and cell-ECM interactions to promote tumor cell invasion and migration.TAMs promote the formation of a microenvironment before metastasis.Evidence shows that the overexpression of CD36 in TAMs increases their ability to take up lipids, which human and mouse tumor tissue macrophages are known to be rich in, and that TAMs can gain energy through FAO, which promotes protumor TAM phenotypes [132].In addition, CD36 deficiency promotes macrophage polarization into the M1 type.Metabolic reprogramming of FAO upregulation in TAMs depends on the induction of peroxisome proliferator-activated receptor γ (PPAR-γ), and PPAR-γ-dependent FAO mediates M2like polarization in tumor-associated macrophages.S100A4 is a metastasis-promoting oncoprotein with intense protumor activities, and its expression levels were found to be highly positively correlated with PPAR-γ activation in TAMs.S100A4-PPAR-γ could promote TAMs to increase FA uptake through CD36, thereby upregulating TAM FAO [133].A study found that CD36 expression is upregulated in metastasis-associated macrophages (MAMs) [134].MAMs contain a higher abundance of lipid droplets and possess a unique ability to engulf long-chain fatty acids derived from tumor cells, which are transported by extracellular vesicles.Through CD36, the lipid-enriched vesicles are selectively taken up by macrophages, providing a fuel source for their metabolism and initiating tumor-promoting activities [134].CD36 plays a vital role in the polarization of TAMs to the M2 type.

CD36 Induces Natural Killer (NK) Cell Dysfunction
NK cells have recently received large amounts of attention as an emerging frontier in cancer cell therapy.Due to their antigen-unrestricted tumor cell-killing mechanism, NK cells not only possess the ability to resist tumor escape, resulting from antigenic drift, but also exhibit the potential to deliver broad-spectrum efficacy against heterogeneous tumor types [135].However, the TME inhibits the antitumor activity of NK cells, and defective NK cell function leads to accelerated tumor formation and growth [136].For instance, one study concluded that lactate in TME inhibits NK cells by decreasing their cytolytic function [137].Lactate could also increase the proportion of MDSCs, a group of cells capable of inhibiting natural killer cytotoxicity [137].After the administration of a high-fat diet to mice, PPAR-α/δ promoted lipid accumulation in NK cells and inhibited mTOR-mediated glycolysis, which is the primary energy pathway for maintaining NK cell function, so that the anti-tumor response of NK cells to melanoma was impaired [138].It has been demonstrated that NK cell dysfunction following cancer surgery contributes to the promotion of metastasis.[139].According to a study, it was discovered that CD36 expression in NK cells was upregulated in mice that underwent surgical treatment [140].This upregulation resulted in enhanced lipid accumulation within NK cells, consequently reducing their ability to effectively lyse tumors in vitro [140].Human colorectal cancer surgery exhibited similar effects on NK cells compared to mouse cancer surgery.These effects included increased lipid content, elevated CD36 expression, reduced granzyme B and perforin production, and impaired cytotoxicity [140].Therefore, gaining a comprehensive understanding of the mechanism underlying lipid accumulation in NK cells could significantly improve cancer prognosis.

CD36 Enhances the Immunosuppressive Effect of Myeloid-Derived Suppressor Cells (MDSCs)
MDSCs are potent immunosuppressive cells of bone marrow origin that generally differentiate into macrophages, dendritic cells, and neutrophils [141].The number of MDSCs in the human body is minimal under normal conditions.However, in pathological states, especially when tumor cells are present in the body, cytokines secreted by tumor cells can promote the production of MDSCs and inhibit the normal differentiation of MDSCs, resulting in the accumulation of MDSCs in the body [142].MDSCs can suppress T-cell-mediated antitumor immunity and suppress NK cell-and macrophage-mediated innate immunity.MDSCs can also induce the proliferation of T reg cells through various pathways, leaving the tumor microenvironment in an immunosuppressed state [143].The metabolic shift from glycolysis to lipid metabolism plays a crucial role in regulating the differentiation and function of various subpopulations of bone marrow cells.Recently, it has been documented that tumor-infiltrating MDSCs predominantly rely on FAO as their primary ATP source, thereby sustaining their immunosuppressive capacity [144].One of the features of this metabolic reprogramming is the increased expression of the lipid uptake receptor CD36 [145].The metabolic transition from glycolysis to lipid metabolism plays a critical role in modulating the differentiation and function of diverse subsets of bone marrow cells.The genetic deficiency of CD36 genes has been shown to result in the decreased uptake of fatty acids and the reduced content of neutral lipids, leading to a decrease in oxidative metabolism.This deficiency has been observed to contribute to the delayed growth of tumors, which is mediated through CD8 + T cells [144].
In summary, the reprogramming of fatty acid metabolism is critical in the progression of malignant tumors.On the one hand, the high expression of CD36 in tumor cells triggers fatty acid uptake and lipid accumulation, which helps promote rapid tumor growth and initiate metastasis.On the other hand, immune cells in the TME overexpress CD36 (Figure 4) and undergo metabolic reprogramming.CD36-mediated FA uptake leads to lipid accumulation and immunosuppressive effects.Therefore, preventing CD36 expression or interfering with its function might become a treatment for tumors or, particularly, an effective strategy for inhibiting tumor metastasis.

CD36 as a Therapeutic Target for Tumors
Currently, many studies have shown that CD36 can be used as a potential target for cancer therapy.There are two reported methods to inhibit the function of CD36 as a lipid transporter: (1) the use of monoclonal antibodies directed to ligand-binding sites; (2) the use of CD36-binding small molecules [146].
Several anti-CD36 antibodies on the market today have been shown to work as CD36 inhibitors to stop tumor progression.Pascual et al. evaluated the role of two CD36 inhibitory antibodies (clone JC63.1 and FA6-152) in an in vivo model of OSCC [44].They treated NSG mice that were inoculated with OSCC in situ with antibodies, showing that this treatment inhibited tumor metastasis.In addition, their treatment with the CD36 antibody was not toxic to the mice.Furthermore, JC63.1 has demonstrated significant results in treating ovarian cancer, gastric cancer, and bladder cancer [87,147,148].Clone Ona-0-v1 also exhibited anticancer effects in ovarian, CRC, and oral cancer mouse models [149].
Another approach to modulating CD36 function is using small molecules that could inhibit the activity of the receptor.Several antagonists have been determined to be effective.Nobiletin, a flavonoid extracted from citrus peel, was found to have antitumor effects on CD36-dependent breast cancer cells [150].In hepatocellular carcinoma cells, sulfo-N-

CD36 as a Therapeutic Target for Tumors
Currently, many studies have shown that CD36 can be used as a potential target for cancer therapy.There are two reported methods to inhibit the function of CD36 as a lipid transporter: (1) the use of monoclonal antibodies directed to ligand-binding sites; (2) the use of CD36-binding small molecules [146].
Several anti-CD36 antibodies on the market today have been shown to work as CD36 inhibitors to stop tumor progression.Pascual et al. evaluated the role of two CD36 inhibitory antibodies (clone JC63.1 and FA6-152) in an in vivo model of OSCC [44].They treated NSG mice that were inoculated with OSCC in situ with antibodies, showing that this treatment inhibited tumor metastasis.In addition, their treatment with the CD36 antibody was not toxic to the mice.Furthermore, JC63.1 has demonstrated significant results in treating ovarian cancer, gastric cancer, and bladder cancer [87,147,148].Clone Ona-0-v1 also exhibited anticancer effects in ovarian, CRC, and oral cancer mouse models [149].
Another approach to modulating CD36 function is using small molecules that could inhibit the activity of the receptor.Several antagonists have been determined to be effective.Nobiletin, a flavonoid extracted from citrus peel, was found to have antitumor effects on CD36-dependent breast cancer cells [150].In hepatocellular carcinoma cells, sulfo-N-succinimidyl oleate (SSO) reduces the EMT phenotype and decreases the migration rate of PA-treated cells by inhibiting CD36 [151].SSO reduces the proliferation of primary CRC cells while increasing cleaved caspase-3 levels and reducing xenograft tumor growth [152].Moreover, 2-methylthio-1,4-naphthoquinone (MTN) is a specific CD36 inhibitor with anticancer activity in GBM [153].
In addition to the methods described above, Jayawardhana et al. designed a Pt(iv) prodrug that mimics the structure of a fatty acid, entering ovarian cancer cells via CD36 and triggering mitochondrial damage, thereby eliminating ovarian cancer cells [154].

Perspectives
The regulation of CD36 expression by peripheral adipocytes, as well as the specific enhancement of metastatic potential in CD36 + metastasis-initiating cells through a high-fat diet, suggests the need to control the quantity and composition of dietary lipids.Concerning daily diet, opting for food choices from the right food categories ensures a balanced and appropriate nutritional intake, thereby offering better alternatives than consuming too little or too much.The prevention of cancer through diet and nutrition is of high practical value.The Mediterranean diet is currently recognized as a healthy diet, characterized by high intakes of vegetables, legumes, fresh fruits, unrefined grains, nuts, and olive oil; moderate intakes of fish and dairy products; low consumption levels of red meat; and moderate consumption levels of alcohol.There is substantial evidence that Mediterranean dietary patterns are negatively associated with cancer risk [155].
In addition to targeting FA uptake, further research is needed to investigate the inhibition of cancer development through other energy source pathways utilized by cancer cells.Additionally, the intervention in tumor development from de novo lipid synthesis pathways requires exploration.Efforts to develop drugs that target tumor metabolism have also gained momentum, with a particular focus on disrupting key metabolic pathways, such as aerobic glycolysis, glutamine metabolism, fatty acid synthesis, nucleotide metabolism, and mitochondrial function.Moreover, preclinical studies of these agents have demonstrated promising results, and several drugs are now being evaluated in clinical trials [156].
The journey toward effective tumor treatment still has a long way to go.More research targeting CD36 and interfering with FA uptake might be an effective strategy for tumor treatment.In addition to CD36 as a target, controlling dietary lipid intake can be a supplementary therapeutic treatment (Figure 5).Reducing lipid intake and replacing saturated and trans fatty acids with foods rich in polyunsaturated fatty acids can help with tumor treatment.People can incorporate this pattern into their daily diet.
search targeting CD36 and interfering with FA uptake might be an effective strategy for tumor treatment.In addition to CD36 as a target, controlling dietary lipid intake can be a supplementary therapeutic treatment (Figure 5).Reducing lipid intake and replacing saturated and trans fatty acids with foods rich in polyunsaturated fatty acids can help with tumor treatment.People can incorporate this pattern into their daily diet.

Figure 1 .
Figure 1.Overview of the relationship among SFAs, MUFAs, PUFAs, and TFAs in cancer.With the exception of ω-3 PUFAs, which can inhibit tumors, most fatty acids have tumor-promoting effects.

Figure 1 .
Figure 1.Overview of the relationship among SFAs, MUFAs, PUFAs, and TFAs in cancer.With the exception of ω-3 PUFAs, which can inhibit tumors, most fatty acids have tumor-promoting effects.

Figure 2 .
Figure 2. Metabolic characteristics of normal cells, tumor cells, and metastatic tumor cells.Normal cells obtain energy by the oxidative phosphorylation of glucose in the mitochondria cloth in the presence of sufficient oxygen, and glycolysis in the cytoplasm in the presence of insufficient oxygen.In addition to using glucose in glycolysis for energy, tumor cells take up free fatty acids via CD36 and utilize fatty acid functions to meet the demands of high proliferation.Metastatic tumor cells demonstrate an augmented metabolic activity, particularly in glucose and fatty acid metabolism, when compared to primary tumors.This metabolic adaptation enables metastatic tumor cells to

Figure 2 .
Figure 2. Metabolic characteristics of normal cells, tumor cells, and metastatic tumor cells.Normal cells obtain energy by the oxidative phosphorylation of glucose in the mitochondria cloth in the presence of sufficient oxygen, and glycolysis in the cytoplasm in the presence of insufficient oxygen.In addition to using glucose in glycolysis for energy, tumor cells take up free fatty acids via CD36 and utilize fatty acid functions to meet the demands of high proliferation.Metastatic tumor cells demonstrate an augmented metabolic activity, particularly in glucose and fatty acid metabolism, when compared to primary tumors.This metabolic adaptation enables metastatic tumor cells to enhance fatty acid uptake and metabolism, thereby utilizing fatty acids as an additional energy source to satisfy their elevated energy requirements.

Figure 3 .
Figure 3.The process of long-chain fatty acids uptake mediated by CD36.

Figure 4 .
Figure 4. High expression of CD36 by immune cells in the tumor microenvironment promotes the uptake of fatty acids and leads to immunosuppression.

Figure 4 .
Figure 4. High expression of CD36 by immune cells in the tumor microenvironment promotes the uptake of fatty acids and leads to immunosuppression.

Figure 5 .
Figure 5. Dietary advice for people who consume too many lipids that cause cancer.Optimize dietary patterns by reducing total lipid intake, increasing polyunsaturated fatty acids, and limiting saturated and trans fatty acids.Simultaneously administer CD36 inhibitors to interfere with the metabolic reprogramming of tumor cells.Implement a diet in conjunction with CD36 inhibitor therapy to inhibit tumor progression and metastasis.