Ethnomedicinal Uses, Geographical Distribution, Botanical Description, Phytochemistry, Pharmacology, and Quality Control of Laportea bulbifera (Sieb. et Zucc.) Wedd.: A Review

Laportea bulbifera (Sieb. et Zucc.) Wedd. (L. bulbifera) is a significant plant in the Laportea genus. Traditionally, it has been used in ethnomedicine for treating various conditions such as rheumatic arthralgia, fractures, falling injuries, nephritis dropsy, limb numbness, pruritus, fatigue-induced internal imbalances, and irregular menstruation. Modern pharmacological studies have confirmed its therapeutic potential, including anti-inflammatory, immunosuppressive, analgesic, and anti-rheumatoid arthritis properties. To gather comprehensive information on L. bulbifera, a thorough literature search was conducted using databases like Web of Science, PubMed, ProQuest, and CNKI. This review aims to provide a comprehensive understanding of L. bulbifera, covering various aspects such as ethnomedicinal uses, geographical distribution, botanical description, phytochemistry, pharmacology, and quality control. The goal is to establish a solid foundation and propose new research avenues for exploring and developing potential applications of L. bulbifera. So far, a total of one hundred and eighty-nine compounds have been isolated and identified from L. bulbifera, including flavonoids, phenolics, nitrogen compounds, steroids, terpenoids, coumarins, phenylpropanoids, fatty acids and their derivatives, and other compounds. Notably, flavonoids and fatty acids have demonstrated remarkable antioxidant and anti-inflammatory properties. Additionally, these compounds show promising potential in activities such as analgesia, hypoglycemia, and hypolipidemia, as well as toxicity. Despite extensive fundamental studies on L. bulbifera, further research is still needed to enhance our understanding of its mechanism of action and improve quality control. This requires more comprehensive investigations to explore the specific material basis, uncover new mechanisms of action, and refine quality control methods related to L. bulbifera. By doing so, we could contribute to the further development and utilization of this plant.

Molecules 2024, 29, x FOR PEER REVIEW 2 of 31 phenylpropanoids [7,8], steroids [1,9,10], aliphatic acids [5,8], nitrogen compounds [8,11], and other compounds.Modern pharmacological studies have demonstrated that extracts and monomeric compounds from L. bulbifera possess anti-inflammatory [12,13], immunosuppressive [14], analgesic [15], and anti-rheumatoid arthritis properties [16], with particular emphasis on its anti-inflammatory and anti-rheumatoid arthritis effects.Among the ethnic medicines in Guizhou Province in southwest China that have been incorporated into the national drug standards, various preparations containing L. bulbifera have been developed.These include Runzao Antipruritic Capsules, Liuwei Shangfuning Ointments, Fufang Shangfuning Ointments, and Tongluo Guzhining Ointments.The cultivation and utilization of L. bulbifera have become crucial endeavors in Guizhou's ethnic medicine pillar industry, possessing distinctive regional resource advantages and development potential [16].Runzao Antipruritic Capsules, in particular, have gained significant popularity in the Chinese market due to their unique therapeutic effect in treating skin itching caused by blood deficiencies in the elderly [17].This inclusion in the Report on the Scientific and Technological Competitiveness of Large Varieties of Traditional Chinese Medicine showcases their popularity [18].Additionally, the young leaves of L. bulbifera are edible, and the stem fibers are durable and suitable for use in textile production [19].
Despite existing research that has summarized the phytochemistry and pharmacology of L. bulbifera [20], there are significant gaps in the coverage.These gaps include the incomplete classification of components, a partial listing of constituents, and the lack of information concerning the chemical structure, exact theoretical molecular weight, and characterization method for these components.Furthermore, the mechanisms underlying the pharmacological effects are often insufficiently detailed and clarified.
In contrast, our review addresses these deficiencies by reporting a total of one hundred and eighty-nine components and providing structural information for each compound, including the name, formula, exact theoretical molecular weight, characterization method, references, and source.Additionally, our review introduces a different classification of pharmacological research compared to the previous report.Importantly, we incorporate the latest research findings on L. bulbifera, resulting in an upto-date and comprehensive perspective.
Therefore, the objective of our review is to bridge these gaps by providing a comprehensive assessment of the ethnomedicinal uses, geographical distribution, botanical description, phytochemistry, pharmacology, and quality control of L. bulbifera.This review aims to serve as a valuable reference for future investigations into L. bulbifera, Among the ethnic medicines in Guizhou Province in southwest China that have been incorporated into the national drug standards, various preparations containing L. bulbifera have been developed.These include Runzao Antipruritic Capsules, Liuwei Shangfuning Ointments, Fufang Shangfuning Ointments, and Tongluo Guzhining Ointments.The cultivation and utilization of L. bulbifera have become crucial endeavors in Guizhou's ethnic medicine pillar industry, possessing distinctive regional resource advantages and development potential [16].Runzao Antipruritic Capsules, in particular, have gained significant popularity in the Chinese market due to their unique therapeutic effect in treating skin itching caused by blood deficiencies in the elderly [17].This inclusion in the Report on the Scientific and Technological Competitiveness of Large Varieties of Traditional Chinese Medicine showcases their popularity [18].Additionally, the young leaves of L. bulbifera are edible, and the stem fibers are durable and suitable for use in textile production [19].
Despite existing research that has summarized the phytochemistry and pharmacology of L. bulbifera [20], there are significant gaps in the coverage.These gaps include the incomplete classification of components, a partial listing of constituents, and the lack of information concerning the chemical structure, exact theoretical molecular weight, and characterization method for these components.Furthermore, the mechanisms underlying the pharmacological effects are often insufficiently detailed and clarified.
In contrast, our review addresses these deficiencies by reporting a total of one hundred and eighty-nine components and providing structural information for each compound, including the name, formula, exact theoretical molecular weight, characterization method, references, and source.Additionally, our review introduces a different classification of pharmacological research compared to the previous report.Importantly, we incorporate the latest research findings on L. bulbifera, resulting in an up-to-date and comprehensive perspective.
Therefore, the objective of our review is to bridge these gaps by providing a comprehensive assessment of the ethnomedicinal uses, geographical distribution, botanical description, phytochemistry, pharmacology, and quality control of L. bulbifera.This review aims to serve as a valuable reference for future investigations into L. bulbifera, as well as offering new insights into the rational utilization of L. bulbifera resources and the efficient development of related products.

Ethnomedicinal Uses
L. Bulbifera, also known as "reib ndad gunb" or "uab detdend" in the Miao language, is widely used as a traditional medicine by ethnic minorities in Guizhou Province, Hubei Province, and Guangxi Zhuang Autonomous Region, China.These communities include the Miao, Buyi, Tujia, Zhuang, and Yao.During the autumn season, the roots are harvested and then sun-dried after removing the stems, leaves, and soil.L. Bulbifera has a pungent flavor and a hot nature, making it suitable for treating conditions related to the cold meridian [21].Its primary functions include clearing the blood network and nervous network [6].For internal use, it is typically decocted with water at a dosage of 9-15 g.When using fresh products, the dosage should be doubled.Alternatively, it can be soaked in Chinese Baijiu.For external application, an appropriate amount can be used for washing or applied externally after being mashed.Its effects encompass dispelling wind and dampness, promoting blood circulation, and removing stasis.It is particularly effective in clearing the food channel, strengthening the spleen, and eliminating accumulated food.Common applications include the treatment of rheumatic arthralgia, fractures, falling injuries, nephritis dropsy, limb numbness, pruritus, fatigue-induced internal imbalances, and irregular menstruation.Additionally, Zhuang doctors often use it to address infantile malnutrition in children and urinary tract stones.The following are some specific prescriptions that involve L. Bulbifera: (1) To treat rheumatism and numbness, decoct 15 g of L. Bulbifera with water, take the water decoction orally, and use the water decoction to wash the affected area.
(2) For rheumatic arthralgia, soak 15 g of L. Bulbifera and 9 g of Acanthopanacis gracilistylus in Chinese Baijiu before consuming.(3) For falling injuries, grind the dried roots into powder and take 6 g of Chinese Baijiu before bedtime.(4) To treat urticaria, decoct 6-9 g of L. Bulbifera with water and take the water decoction orally.For pediatric use, the dosage should be appropriately reduced.(5) To alleviate body deficiency and swelling, take 9-15 g of L. Bulbifera and 250 g of pork.Stew them together and consume the soup and meat once a day for 2-3 days.( 6) For cough, decoct 20-30 g of L. Bulbifera with water and take the water decoction orally.(7) For anemofrigid cold and cough, decoct 30 g of L. Bulbifera with water and take the water decoction orally [21].

Geographical Distribution
L. bulbifera is distributed across various regions in China, including Heilongjiang, Jilin, Liaoning, Shandong, Hebei, Shanxi, Henan, Anhui, Zhejiang, Fujian, Taiwan, Jiangxi, Hubei, Hunan, northern Guangdong, Guangxi, Guizhou, Yunnan, Xizang, Sichuan, Gansu, and Shaanxi.Figure 2 illustrates the general geographical distribution of L. bulbifera in China.It can also be found in Japan, North Korea, Russia, Sikkim, India, Sri Lanka, and Java Island in Indonesia.This plant grows in hillside forests and on semi-shady slopes at altitudes of 1000-2400 m [22].

Botanical Description
The female perianth has four segments, and the male perianth has 4-5 segments.The ovary has a pistil stalk, and the stigma is filiform, measuring 2-4 mm in length.Initially, the ovary is upright and later becomes oblique.The achenes are round and obovate or nearly semicircular, oblique, flat, and 2-3 mm long with purplish-brown spots.The pistil

Botanical Description
The female perianth has four segments, and the male perianth has 4-5 segments.The ovary has a pistil stalk, and the stigma is filiform, measuring 2-4 mm in length.Initially, the ovary is upright and later becomes oblique.The achenes are round and obovate or nearly semicircular, oblique, flat, and 2-3 mm long with purplish-brown spots.The pistil stalk is retroflex, and two persistent perianth segments extend to the middle of the fruit.The fruit stalk has membranous wings, and sometimes, the fruit inflorescence is branched and winged, spoon-shaped, with a concave top.The flowering period is from June to August, and the fruiting period is from August to December [22,23].
L. bulbifera is a perennial herb.The root of L. bulbifera is long and conical or slender, spindle-shaped, and twisted, with a length ranging from 6 to 20 cm and a diameter of 3-6 mm.The surface has a grayish-brown to reddish-brown color, with fine longitudinal wrinkles and slender fibrous roots or fibrous root scars.It has a hard texture and is not easily broken, with a fibrous cross-section and a light reddish-brown color [21].The stem is 0.4-1.5 m tall, with short hairs and a few stinging hairs.The bulbils are almost spherical, with a diameter of 3-6 mm.The leaves are alternate, ovate, elliptical, or lanceolate, measuring 8-16 cm in length and 3-6 cm in width.The apex is acuminate, the base is broadly cuneate or circular, and the margin is densely toothed.The lower surface is sparsely covered with short hairs and stinging hairs.Cystoliths are punctate, with three basal veins and 4-6 pairs of lateral veins.The petiole is 1.5-6 cm long, and the stipules are oblong-lanceolate, measuring 0.5-1 cm in length and being two-lobed.The inflorescence is paniculate, and the plant is monoecious.The male inflorescence is located in the upper leaf axil of the stem and measures 3-10 cm in length, while the female inflorescence is located at or near the top leaf axil, measuring 10-25 cm in length with a peduncle of 5-12 cm.The female perianth has 4 segments, and the male perianth has 4-5 segments.The ovary has a pistil stalk, and the stigma is filiform, measuring 2-4 mm in length.Initially, the ovary is upright and later becomes oblique.The achenes are round, obovate, or nearly semicircular, oblique, flat, and 2-3 mm long with purplish-brown spots.The pistil stalk is retroflex, and two persistent perianth segments extend to the middle of the fruit.The fruit stalk has membranous wings, and sometimes, the fruit inflorescence is branched and winged, spoon-shaped, with a concave top.The flowering period is from June to August, and the fruiting period is from August to December [22,23].

Phytochemistry
Over the years, numerous active compounds have been isolated and identified from the aerial parts or roots of L. bulbifera, particularly in recent times.As the importance and utilization of this plant increase, research on its components has also expanded.According to reports, a total of one hundred and eighty-nine compounds have been isolated or identified from L. bulbifera.These compounds can be categorized into nine groups, including flavonoids, phenolics, nitrogen compounds, steroids, terpenoids, coumarins, phenylpropanoids, fatty acids and their derivatives, as well as other compounds.This remarkable abundance of bioactive ingredients in L. bulbifera highlights its potential as a source for drug development and clinical applications.

Flavonoids
Among the compounds derived from L. bulbifera, flavonoids have received the most extensive research and were the earliest reported type.A total of fifty-one flavonoid components have been identified, consisting of twenty-three flavonoids and twenty-eight flavonoid glycosides (Table 1, Figure 3).The team from Dalian University isolated nine flavonoids and their glycosides from the aerial parts and the whole herb of L. bulbifera, respectively [2,24].Additionally, five flavonoids were isolated from the aerial parts [11], while twenty-six flavonoids and their glycosides were obtained from the roots of L. bulbifera through bioassay-guided isolation [1].Furthermore, HPLC-MS technology was used to identify seven flavonoids and their glycosides from the 95% ethanol extract of the roots [2].
Epigallocatechin was isolated from the whole herb [25], and rutin was isolated from the aerial parts [26].Two flavonoid glycosides were also obtained from the whole herb [10], and four flavonoids and their glycosides were identified through UHPLC-ESI-Q-TOF-MS technology from the 70% ethanol extract [7].Similarly, We employed the same technique to identify two flavonoid glycosides from the methanol extract of the roots [27].UV: Ultraviolet spectrophotometry; 13 C NMR: Carbon-13 nuclear magnetic resonance spectrometry; 1 H NMR: Hydrogen-1 nuclear magnetic resonance spectrometry; HPLC-MS: High-performance liquid chromatographymass spectrometry; HR-MS: High-resolution mass spectrometry; mp: Melting point; ESI-MS: Electrospray ionization mass spectrometry; UHPLC-ESI-Q-TOF-MS: Ultra high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry.
Flavonoids are natural polyphenolic substances and secondary metabolites of plants.They possess remarkable antioxidant activity, which has been extensively investigated.This antioxidant activity aids in the prevention of damage caused by free radicals through scavenging reactive oxygen species (ROS), activating antioxidant enzymes, and inhibiting oxidases.Moreover, flavonoids elevate uric acid levels and exhibit metal-chelating activity to alleviate oxidative stress [28].Studies have also indicated that flavonoids activate antioxidant pathways, thereby contributing to their anti-inflammatory effects.They inhibit the secretion of enzymes such as lysozymes and β-glucuronidase, as well as the secretion of arachidonic acid, thus reducing inflammatory reactions.Flavonoids such as apigenin (3), kaempferol (7), and (−)-epigallocatechin 3-O-gallate (22) play a role in modulating the expression and activation of various cytokines, including interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8).They also regulate the gene expression of several pro-inflammatory molecules, such as nuclear factor-kappaB (NF-κB), activator protein-1 (AP-1), and intercellular adhesion molecule-1 (ICAM).Additionally, they inhibit pro-inflammatory enzymes such as inducible nitric oxide (NO) synthase, cyclooxygenase-2, and lipoxygenase [29].Flavonoids are natural polyphenolic substances and secondary metabolites of plants.They possess remarkable antioxidant activity, which has been extensively investigated.This antioxidant activity aids in the prevention of damage caused by free radicals through scavenging reactive oxygen species (ROS), activating antioxidant enzymes, and inhibiting oxidases.Moreover, flavonoids elevate uric acid levels and exhibit metal-chelating activity to alleviate oxidative stress [28].Studies have also indicated that flavonoids activate antioxidant pathways, thereby contributing to their anti-inflammatory effects.They inhibit the secretion of enzymes such as lysozymes and β-glucuronidase, as well as the secretion of arachidonic acid, thus reducing inflammatory reactions.Flavonoids such as apigenin (3), kaempferol (7), and (-)-epigallocatechin 3-O-gallate ( 22) play a role in

Fatty Acids and Their Derivatives
A total of forty-five fatty acids and their derivatives were isolated and identified from various parts of L. bulbifera, including the roots, aerial parts, and the whole herb (Table 8, Figure 10).These include saturated and unsaturated fatty acids, hydroxy fatty acids, amino fatty acids, fatty esters, and fatty amides.Fatty acids have shown potential in treating metabolic diseases such as type II diabetes, inflammatory diseases, and cancer [39,40].Intake of linoleic acid (121) has been found to improve hyperlipidemia and reduce the incidence of type II diabetes [41].Linolenic acid (133) possesses anti-metabolic syndrome, anticancer, anti-inflammatory, and antioxidant properties [42].Neochlorogenic acid (110), chlorogenic acid (111), and 4-O-caffeoylquinic acid have been identified from the 70% ethanol extract of the roots and the whole herb [7,26].Caffeic acid cinnamyl ester (114), secoisolariciresinol 9-O-β-D-glucopyranoside (115), and (E)-4coumaric acid (116) have been identified from the methanol extract of the roots by us [27].Caffeic acid (106) has also been isolated from the roots [30].Chlorogenic acid (111) is a significant compound with antioxidant, hepatoprotective, cardioprotective, anti-inflammatory, and free radical scavenging activities.Moreover, it has been found to modulate lipid metabolism and glucose levels [36].Caffeic acid (106) is another important compound known for its antioxidant, immunomodulatory, and anti-inflammatory activities [37].Danshensu (108) exhibits effects such as antioxidant properties, inflammation regulation, and lipidemia control [38].

Fatty Acids and Their Derivatives
A total of forty-five fatty acids and their derivatives were isolated and identified from various parts of L. bulbifera, including the roots, aerial parts, and the whole herb (Table 8, Figure 10).These include saturated and unsaturated fatty acids, hydroxy fatty acids, amino fatty acids, fatty esters, and fatty amides.Fatty acids have shown potential in treating metabolic diseases such as type II diabetes, inflammatory diseases, and cancer [39,40].Intake of linoleic acid (121) has been found to improve hyperlipidemia and reduce the incidence of type II diabetes [41].Linolenic acid (133) possesses anti-metabolic syndrome, anticancer, anti-inflammatory, and antioxidant properties [42].

Quality Control
For a long time, L. bulbifera has mainly relied on wild resources.However, with the increasing popularity of traditional Chinese medicine based on it, the demand has been growing year by year.Simultaneously, the wild resources have been gradually depleted, and their quality is inconsistent, thus failing to meet the application needs.Therefore, it is crucial to conduct prompt research on quality control.It is worth mentioning that the "Quality Standards for Traditional Chinese Medicine and Ethnomedicine in Guizhou Province" includes documentation on the whole herb of L. bulbifera.This standard only provides information on its name, source, characteristics, identification, nature and flavor, channel tropism, main functions, usage, dosage, and storage.Among these, microscopic identification and thin-layer chromatography (TLC) are used for identification, with βsitosterol serving as the reference substance [43].Nevertheless, the level of quality control is relatively low because β-sitosterol is not a characteristic compound and cannot represent the medicinal material's quality.
Pharmacognostic research on L. bulbifera has been conducted in various studies [44,45].These studies involve morphological identification; microscopic identification of roots, stems, and leaves; and the use of isorhamnetin-3-O-α-L-rhamnopyranosyl-(1-2)-βgalactopyranoside (47) as a characteristic compound.Furthermore, a characteristic fingerprint of L. bulbifera was established using HPLC to effectively differentiate it from similar varieties [45].Researchers have also developed TLC and HPLC methods utilizing rutin (48) as the characteristic component.By determining the rutin content in L. bulbifera from different regions, they are able to evaluate the medicinal material's quality [26].Additionally, a study has established an HPLC method for the determination of multiple indicators: epicatechin (8), catechin ( 9), (−)-gallocatechin (12), and epigallocatechin (13) in L. bulbifera.This simple method could be employed for the quality control of L. bulbifera [25].Furthermore, there are reports on the simultaneous determination of eleven components (flavonoids and phenylpropanoids) in L. bulbifera using UHPLC-ESI-MS, which could be utilized for quality control [46].This method is currently the most comprehensive for quality control purposes.Researchers have also examined the content of total active ingredients in L. bulbifera, such as total flavonoids [26], total polysaccharides [47], or total coumarins, to evaluate the medicinal material's quality [48].Moreover, scholars have investigated quality-related parameters, including water content, total ash content, acid-insoluble ash content, ethanol-soluble extractives, heavy metals, harmful elements, and organochlorine pesticide residues in L. bulbifera [25,26].
Research has shown that L. bulbifera is rich in coumarins and exhibits significant therapeutic effects on arthritis [16].Moreover, studies indicate a high content of catechins in L. bulbifera, resulting in notable anti-inflammatory effects [1].Additionally, research findings demonstrate that L. bulbifera has a high flavonoid content and diverse flavonoid types, displaying potent antioxidant activity [11].Nevertheless, there is significant variation in the results of these studies on active ingredients, with minimal intersections.The underlying reason for this outcome remains unclear and may be attributed to differences in the origin, medicinal parts, and processing methods of L. bulbifera.Future research should focus on strengthening the investigation of its chemical components to elucidate the compounds responsible for its pharmacological effects.Consequently, a correlation model based on spectral efficacy was established to identify quality markers that better reflect the quality of L. bulbifera.

Pharmacological Effects
As a medicinal plant, modern pharmacological studies have demonstrated various pharmacological effects of L. bulbifera, including antioxidant, anti-inflammatory, analgesic, hypoglycemic, and hypolipidemic activities, as well as toxicity.

Anti-Inflammatory and Analgesic Effects
Inflammation is a common pathological process in clinical practice.It is a defensive response that the body generates after tissue damage or invasion by pathogenic factors.It is essential for the occurrence and development of many diseases.Therefore, research on anti-inflammatory drugs is highly significant [49].The ethyl acetate extracts (100 µg/mL) derived from the roots of L. bulbifera demonstrated significant inhibitory activity against cyclooxygenase-2 (COX-2), with an inhibitory rate of 60.7%.Out of the forty-six compounds (10 µM) isolated from the ethyl acetate extract, twenty-three compounds exhibited inhibitory rates higher than 50%.Among these, thirteen compounds displayed strong inhibitory activity with IC 50 values lower than 1 µM.Notably, compounds such as (−)epicatechin-3-O-gallate (21), hyperoside (40), rutin (48), quercetin (11), fisetin (6), and luteolin (5) (with IC 50 values ranging from 0.13 to 0.24 µM) showed optimal COX-2 inhibitory potency.The inhibitory activity of flavonoids against COX-2 is influenced by the number and position of phenolic hydroxyl groups [1].
In a study using lipopolysaccharide (LPS) to stimulate a mouse macrophage RAW264.7 model, the effects of different extracts from L. bulbifera roots on NO release and their antiinflammatory activity were examined.Results revealed that the dichloromethane extract, ethyl acetate extract, and n-butanol extract at concentrations of 15.5, 31.25, and 62.5 µg/mL, respectively, exerted a significant impact on NO release, with statistically significant differences observed.At a concentration of 62.5 µg/mL, the inhibitory effects of the petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract on NO release were 11.42%, 21.01%, 33%, and 26.96%, respectively.Specifically, the ethyl acetate extract exhibited the most pronounced effect on NO release, and its impact was dosedependent, demonstrating excellent anti-inflammatory activity [30].Inflammatory cell models (RAW264.7)were utilized to evaluate the anti-inflammatory activities.Additionally, the petroleum ether extract (0.2, 2, 20 µg/mL) from L. bulbifera was assessed for its TNF-α inhibition activity.Further analysis is warranted for the thirty-five batches of petroleum ether extract exhibiting therapeutic effects under 2 µg/mL [13].Several reports have explored the use of total coumarins derived from L. bulbifera roots (20, 40, and 60 mg/kg) to treat type II collagen-induced arthritis in Balb/c mice.The results demonstrated that treatment with total coumarins (60 mg/kg) led to a significant and dose-dependent reduction in clinical arthritis score and paw swelling.Pathological changes indicated that total coumarins protected tissues against bone destruction.This protective effect was associated with a considerable decrease in the production of IFN-γ and IL-2, an increase in IL-10 and TGF-β, and the suppressive expression of T-bet in dendritic cells.Additionally, total coumarins induced the generation of CD4 + CD25 + Treg cells expressing the Foxp3 phenotype.The dendritic cells treated with total coumarins displayed low expression of MHC class II and CD86 molecules, as well as reduced levels of IL-12p70.In summary, total coumarins exhibit significant protective effects and warrant further investigation and development as a potential anti-arthritis drug [16].
To evaluate the anti-rheumatoid arthritis effects of the serum, the human rheumatoid arthritis fibroblast-like synoviocyte line MH7A was cultured and treated with TNF-α (50 ng/mL) in vitro.The serum containing the whole herb of L. bulbifera was used to determine the proliferation and levels of inflammatory cytokines, such as prostaglandin E2 (PGE2), IL-1β, and IL-6, in the MH7A cells.The active components were identified based on the peak areas of common peaks and the results of the anti-rheumatoid arthritis effect test.The serum containing L. bulbifera significantly inhibited the proliferation of TNF-αactivated MH7A cells and the expression of PGE2, IL-6, and IL-1β.Thirty newly generated compounds were detected in the drug-containing serum.Among them, eight components were determined to enter the bloodstream as prototypes, and twelve components showed significant correlation with the pharmaceutical effect.Neochlorogenic acid (110), cryptochlorogenic acid (112), and chlorogenic acid (111) made significant contributions to the anti-rheumatoid arthritis activity [50].
The results of the experiment on anti-inflammatory activity showed that the swelling inhibition rate in mice treated with the 70% ethanol extract (20 g raw medicine/kg) of the whole herb from L. bulbifera was comparable to that of the positive group, with an inhibition rate greater than 50%.This inhibitory effect was better than that of the water extract.The test on analgesic activity showed that both the 70% ethanol extract group (20 g raw medicine/kg) and the water extract group (20 g raw medicine/kg) from the whole herb of L. bulbifera had an inhibitory effect on the number of twisting times in mice, but the former had a better effect.The experimental results also demonstrated that the pain threshold of mice increased by 34.2% after administration of the 70% ethanol extract (20 g raw medicine/kg), indicating its superior central analgesic effect caused by thermal stimulation compared to that of the water extract [25].Studies also revealed that the ethyl acetate extract of L. bulbifera obtained similar results.It was found that the ethyl acetate extract could dose-dependently inhibit the proliferation of splenic T lymphocytes and the secretion of IL-2 and IFN-γ in the cell culture supernatant.These findings indicate that the ethyl acetate extract has a certain immunosuppressive effect and serves as the material basis for L. bulbifera's anti-rheumatoid arthritis effect [15].
A study investigated the differences in intestinal absorption characteristics of L. bulbifera extract between normal and rheumatoid arthritis pathological states in rats.The absorption concentration of L. bulbifera extract was 5.0 mg/mL, and the UHPLC-MS/MS technique was used to detect the content of eight indicator components in the extract.The results revealed that all eight indicator components in the extract could be absorbed into the intestinal sac in a linear manner.The cumulative absorption time curve for each component showed a progressive increase without reaching saturation, suggesting a zero-order absorption rate process.It is suggested that the possible absorption mode for each component is passive diffusion, which provides a theoretical foundation for the development of oral dosage forms.Under normal conditions, the ileum (except for chlorogenic acid) exhibited the highest absorption of various components, while under pathological conditions, the duodenum showed the highest absorption.Additionally, the overall absorption of the eight components in each intestinal segment of rats with rheumatoid arthritis was higher than that of normal rats, suggesting that rheumatoid arthritis may alter the specific site of drug absorption [51].
In another study, the inhibitory effect of four isolated steroids from the whole herb of L. bulbifera on NO activity was evaluated using a mouse RAW264.7 cell model.The results indicated that the four steroid compounds (50 µg/mL) significantly reduced the production of NO in the model cells, with inhibition rates ranging from 27.41% to 40.10%.Among them, ergosterone exhibited the highest efficacy, suggesting that steroids may contribute to the anti-inflammatory properties of L. bulbifera [10].
A study utilized the LPS assay to determine the average anti-inflammatory activity of different polar extracts (1 mg/mL).The results showed that the petroleum ether extract (15.38%) exhibited the highest anti-inflammatory activity, followed by the ethyl acetate extract (7.91%) and the water extract (2.60%).The petroleum ether extract was identified as the active anti-inflammatory extract of L. bulbifera using SPSS software (Version 16.0) for variance analysis [26].Another report also confirmed the potent anti-inflammatory effects of the petroleum ether extract [13].Research findings suggest that (E)-4-coumaric acid (116) and caffeic acid (106) in L. bulbifera possess anti-inflammatory activity and can be absorbed into the bloodstream.These components are likely to be the effective anti-inflammatory compounds of L. bulbifera [8].
The results of a different study demonstrated that the ethyl acetate extract from L. bulbifera (at concentrations of 0.5, 1.0, and 1.5 mg/10 g) effectively inhibited the onset of inflammation and joint tissue lesions.It exhibited a favorable therapeutic effect on rheumatoid arthritis, as evidenced by the arthritis index, arthritis incidence rate, spleen index, toe swelling, and pathological photos.The ethyl acetate extract (at concentrations of 0.5, 1.0, and 1.5 mg/10 g) did not influence changes in surface antigens of dendritic cells, but it reduced the expression of T-bet and inhibited IFN-γ secretion while promoting IL-10 secretion.It also affected T cells by inhibiting T-bet expression and promoting GATA-3 expression, thereby enhancing the secretion of IL-4 and IL-10 while inhibiting the expression of IFN-γ and IL-2 to prevent the onset of rheumatoid arthritis [52].
In a study investigating the effects of total coumarins from L. bulbifera on mice with dextran sulfate sodium-induced colitis, it was found that intervention with different doses of total coumarins (37.5, 75, 150 mg/kg) significantly improved colitis symptoms.This improvement was characterized by stable weight gain, reduced damage to the intestinal mucosa, decreased infiltration of inflammatory cells, and the absence of diarrhea or bloody stools.Further research revealed that total coumarins were able to regulate the expression of pro-inflammatory and anti-inflammatory cytokines, as well as reduce the levels of TLR4 and NF-κB in colon tissue.Moreover, no common adverse reactions such as weight loss, infection, or organ damage were observed during the administration of total coumarins.Therefore, this study provides a theoretical foundation for the development and usage of total coumarins of L. bulbifera as immunosuppressants [12].
In another study, the immunosuppressive effects of the ethyl acetate extract from L. bulbifera were investigated in a murine model of skin allograft rejection.The model involved transplanting skin allografts from C57BL/6 mice onto the wound bed of Balb/c mice.The results demonstrated a significant dose-dependent prolongation of skin allograft survival in animals treated with the ethyl acetate extract.FACS analysis revealed that treatment with the extract (200 mg/kg) led to an immature state of dendritic cells and stimulated the differentiation of CD4 + CD25 + Tregs.Moreover, the extract efficiently reduced T-bet gene expression and spleen lymphocyte proliferation in treated mice.In comparison to the model control, recipients treated with the extract exhibited significant downregulation of Th1 cytokines (IL-2, IFN-γ) and a notable increase in Th2 cytokine (IL-10) levels in the serum, with a dose-related pattern.These findings suggest that the ethyl acetate extract has anti-allograft rejection properties by promoting CD4+CD25+ Tregs differentiation and maintaining the immaturity of dendritic cells, thereby inducing a stable immunological tolerance state.This highlights its potential for the treatment of autoimmune diseases [14].

Hypoglycemic and Hypolipidemic Activity
To investigate the effects of total coumarins on diabetes, eight-week-old non-obese diabetic (NOD) mice were divided into four groups: a control group and low-dose (37.5 mg/kg), middle-dose (75 mg/kg), and high-dose (150 mg/kg) total coumarin treatment groups.The results demonstrated that treatment with total coumarins for four weeks significantly inhibited insulitis, increased pancreatic islet number, delayed the onset, and reduced the development of diabetes by twenty-six weeks of age in NOD mice compared to untreated control mice.Total coumarins also suppressed spleen T-lymphocyte proliferation, induced a Th2-biased cytokine response, promoted the generation of CD4 + CD25 + Foxp3 + Tregs, and increased Foxp3 mRNA expression.Furthermore, dendritic cells treated with total coumarins exhibited low expression of MHC class II and CD86 molecules.The expressions of the TLR4 gene and protein expressions in the spleen, thymus, and pancreas were downregulated in the groups treated with total coumarins.Key molecules involved in the downstream signaling cascades of TLR4, such as myeloid differentiation factor 88 (MyD88), NF-κB, IL-1β, TRIF, TRAM, IRF-3, and IFN-β, all showed significant decreases in the total coumarins groups.This suggests that total coumarins inhibit both MyD88-dependent and -independent pathways of TLR4.At the cellular level, TLR4 protein expression was found to be downregulated by total coumarins in dendritic cells but not in Tregs.Furthermore, total coumarins enhanced the role of dendritic cells, rather than Tregs, in negative immune regulation in vitro.This effect on dendritic cell immune function was reversed by anti-TLR4 antibody.Therefore, the total coumarins from L. bulbifera can prevent autoimmune diabetes in mice by inhibiting the TLR4 signaling pathway [53].
To establish a model of insulin resistance type II diabetes, BALB/c mice were fed a high-fat diet and injected with small doses of STZ.The effects of different concentrations of total flavonoids of L. bulbifera (25, 50, 100 mg/kg) on the blood glucose concentration of the diabetic model were observed through daily intragastric administration.The results indicated that the total flavonoids group significantly reduced blood sugar levels in mice compared to the model group.Pancreatic HE staining showed no significant difference between the groups.The low-dose group demonstrated a significant effect in reducing triglycerides, total cholesterol, and the insulin resistance index.It also improved glucose tolerance in insulin-resistant mice.Insulin measurement results showed a significant increase in insulin levels only in the high-dose group.SOD and MDA levels did not show significant changes in any of the groups.Additionally, immunoblotting results for insulin receptors and PPAR-γ showed that the low-dose group of total flavonoids increased the expression of insulin receptor levels.These results demonstrate that total flavonoids exert a hypoglycemic and hypolipidemic effect by upregulating insulin receptor levels and increasing insulin sensitivity rather than affecting the free radical pathway [54].
In another study, male Kunming mice were fed a high-fat diet for two weeks to establish a model of hypercholesterolemia. L. bulbifera was extracted and separated using macroporous resin to obtain four fractions: water fraction, 30% ethanol fraction, 70% ethanol fraction, and 95% ethanol fraction.Each fraction was administered by gavage at a dose of 40 mg/g, and serum biochemical indicators were measured after four weeks.Liver sections were stained for observation.The experimental results showed that both the 30% ethanol fraction and 70% ethanol fraction significantly reduced body weight and serum levels of total cholesterol, low-density lipoprotein cholesterol, and MDA in hypercholesterolemic mice.They also increased the levels of SOD in experimental hypercholesterolemic mice.Staining results of mouse liver cells revealed that the liver tissue sections of mice treated with the 30% ethanol fraction and 70% ethanol fraction showed normal liver cells around the central vein, indicating that these fractions could protect and repair the liver tissue of hypercholesterolemic mice.In summary, the 30% ethanol fraction and 70% ethanol fraction of L. bulbifera could regulate blood lipid metabolism in experimental hypercholesterolemic mice and significantly reduce their blood lipid levels [5].

Toxicity
There are records indicating that L. Bulbifera has minor toxicity, although ethnic doctors generally consider it non-toxic [55].Research reports have demonstrated that the oral administration of a water decoction and powder suspension of L. bulbifera to mice exhibited a minimum lethal dose greater than 50 g/kg and 1.67 g/kg, respectively [44].In our previous oral acute toxicity experiments, we observed high safety when mice were administered L. bulbifera via gavage (2000 g/kg), as no mouse deaths occurred within 24 h [27].

Discussion
Firstly, this manuscript provides a comprehensive overview of the chemical composition of L. bulbifera, a traditional ethnomedicine.The analysis reveals that L. bulbifera is abundant in flavonoids and fatty acids, two crucial phytochemicals known for their potent antioxidant properties.These compounds exhibit the ability to neutralize free radicals, thereby mitigating cellular damage caused by oxidative stress [28,42].Moreover, they also possess significant anti-inflammatory effects by effectively suppressing the release of inflammatory pathways and cytokines [29,56].In fact, studies have found that flavonoids and phenolics could effectively ameliorate rheumatoid arthritis, a chronic inflammatory disorder [57].Additionally, evidence suggests that fatty acids play a vital role in the prevention and treatment of rheumatoid arthritis [58].Therefore, considering the aforementioned findings, it could be inferred that the therapeutic effects of L. bulbifera in mitigating rheumatic arthritis, fractures, and falling injuries are primarily attributed to its rich content of flavonoids and fatty acids.
Additionally, two important issues related to quality control need to be addressed.Firstly, there is variation in the methods used to determine the chemical components in L. bulbifera.Different compounds, such as β-sitosterol [43], flavonoids (isorhamnetin-3-Oα-L-rhamnopyranosyl-(1-2)-β-galactopyranoside (47), rutin (48) [26], and catechins [25]), and flavonoids in combination with phenylpropanoids [46], have been measured to assess the quality of L. bulbifera.However, these research studies lack systematicity, making it unclear which components truly reflect the quality of L. bulbifera.Secondly, the established indicators for quality control of L. bulbifera have not been based on their pharmacological substance basis and quality markers.As a result, the exclusive analysis of active ingredients is lacking, compromising the ability to accurately reflect and evaluate the quality of L. bulbifera.Given the increasing market demand for L. bulbifera, ensuring its safety and effectiveness from the source is crucial.To achieve this, researchers should explore the anti-inflammatory material basis of L. bulbifera, clarify its mechanism of action, and establish the relationship between its anti-inflammatory spectrum and effects.It is also important to screen and identify quality biomarkers that can faithfully represent the quality of L. bulbifera.Addressing these issues is vital in maintaining the stable and reliable quality of L. bulbifera, thus meeting the growing demand for this medicinal plant.
Furthermore, coumarins and flavonoids have been identified as significant components in the treatment of arthritis and inflammation, respectively [1,11,16].These two phytochemicals exhibit distinct active properties, indicating that they play different roles in the treatment process.Therefore, we believe that the origin and specific medicinal parts of L. bulbifera represent the primary influencing factors.It is well-known that numerous environmental elements, including growth conditions, geographical location, and habitat, can result in variations in plant composition.Factors like plant growth environment, soil quality, climate conditions, and light intensity may vary across different regions, leading to diverse chemical compositions and contents in the same plant species.Consequently, medicinal plants grown in different habitats may exhibit dissimilar ingredient profiles and quantities, potentially resulting in varied pharmacological and clinical effects within different regions.Additionally, the medicinal parts utilized can significantly impact the therapeutic outcomes.Our previous investigations, supported by the literature, have demonstrated that the roots possess superior antioxidant capacity compared to the aerial parts [27].However, previous studies have employed a variety of medicinal parts, including roots [1], aerial parts [11], and the whole herb [25], contributing to disparate findings.
Moving forward, several crucial avenues of research should be pursued regarding L. bulbifera.Firstly, a more extensive exploration of its chemical composition is warranted to elucidate the specific substances responsible for its pharmacological effects.Secondly, a comprehensive analysis of its pharmacological mechanisms should be conducted to offer theoretical guidance and technical support for drug development and clinical application.Subsequently, quality control measures must be implemented to ensure the consistency and reliability of therapeutic effects.Finally, it is essential to systematically validate and optimize traditional applications of L. bulbifera, harnessing its full potential and broadening its prospects for practical use.

Conclusions
However, there is currently a lack of comprehensive and detailed documentation on the ethnomedicinal uses, geographical distribution, botanical description, phytochemistry, pharmacology, and quality control of L. bulbifera.Consequently, the primary objective of this review is to comprehensively explore existing research on L. bulbifera by examining multiple databases and addressing these aforementioned aspects.Furthermore, this review will identify potential areas for future research, such as isolating and identifying additional compounds found in L. bulbifera, conducting more extensive pharmacological evaluations, elucidating its mechanisms of action, and ultimately establishing a more robust quality control system.The outcomes of this research will serve as a solid basis for the quality control, product development, and clinical application of L. bulbifera.

Figure 2 .
Figure 2. The general geographical distribution of Laportea bulbifera in China.

Figure 3 .
Figure 3.Chemical structures of flavonoids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 3 .
Figure 3.Chemical structures of flavonoids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 4 .
Figure 4.Chemical structures of phenolics isolated from Laportea bulbifera.Chemical structures were drawn using ChemdDaw Professional 15.0 software.

Figure 4 .
Figure 4.Chemical structures of phenolics isolated from Laportea bulbifera.Chemical structures were drawn using ChemdDaw Professional 15.0 software.

Figure 5 .
Figure 5.Chemical structures of nitrogen compounds isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 5 .
Figure 5.Chemical structures of nitrogen compounds isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 6 .
Figure 6.Chemical structures of steroids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 7 .
Figure 7.Chemical structures of terpenoids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 7 .
Figure 7.Chemical structures of terpenoids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 8 .
Figure 8.Chemical structures of coumarins isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 8 .
Figure 8.Chemical structures of coumarins isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 9 .
Figure 9.Chemical structures of phenylpropanoids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 9 .
Figure 9.Chemical structures of phenylpropanoids isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 10 .
Figure 10.Chemical structures of fatty acids and their derivatives isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 10 .
Figure 10.Chemical structures of fatty acids and their derivatives isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 11 .
Figure 11.Chemical structures of others isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Figure 11 .
Figure 11.Chemical structures of others isolated from Laportea bulbifera.Chemical structures were drawn using ChemDraw Professional 15.0 software.

Table 8 .
Fatty acids and their derivatives isolated from Laportea bulbifera.