Borane-Pyridine: An Efficient Catalyst for Direct Amidation

Borane-pyridine acts as an efficient (5 mol%) liquid catalyst, providing improved solubility for the direct amidation of a wide range of aromatic and aliphatic carboxylic acids and amines to form secondary and tertiary carboxamides. Tolerance of potentially incompatible halo, nitro, and alkene functionalities has been demonstrated.


Introduction
Amide functionality plays an undeniably important role in biochemistry and pharmaceutical chemistry [1].An appreciable number of major medicines and natural products contain amide bonds [2].The challenge of amide formation is still a pressing objective as their synthesis is still one of the most frequently used and practiced transformations in process and medicinal chemistry [3].A variety of techniques have been implemented to increase the reactivity of carboxylic acids to enhance amidation such as activation via acid chlorides, anhydrides, imidazolides, carbodiimides, thioesters and other coupling reagents [2].Silane reagents have been documented recently as efficient amidating tools with tetramethylorthosilicate (TMOS) and methyltrimethoxysilane (MTM) having been explored for this purpose [4].Boron-based amidation reagents, though known for some time, have recently been taken up again and studied extensively for direct amidation [5].Many of the boron-based reagents are catalytic, avoiding one of the most obvious drawbacks with stoichiometric reagents: the large quantity of waste produced.
Pioneering work in boronic acid catalyzed direct amidation was performed by Yamamoto, with additional developments and improvements made by Blanchet [6], Hall [7], Whiting [8], and more recently Xian-Bin [9] as well as Liu, who utilized boronic acids as starting materials for amide synthesis [10].Boronic ester and boric acid mediated reactions have also been explored, with Sheppard making the use of tris(2,2,2-trifluoro) borate and other simple borate esters [11] and Tang utilizing boric acid [12].Triacyloxyborane intermediates formed through the reaction between a carboxylic acid and borane-tetrahydrofuran [13] or borane-trimethylamine [14] (Scheme 1A) were also found to be sufficiently activated to enable amidation.
Our recent focus and success in exploring borane-amines in a wide variety of reactions led us to further investigate their application in carboxamide synthesis.While boraneamines have been demonstrated to be viable reductants for various functional groups, including carboxylic acids [15] and amides [16], these reactions require a catalyst to proceed.The potentially competing reduction was not found to be an issue in our recently reported direct amidation of carboxylic acids and borane-amines, wherein the amine coordinated to boron was incorporated into the product amide (Scheme 1B) [17].Our subsequent report on the use borane-ammonia (BH 3 NH 3 , 1a) as an efficient sub-stoichiometric amidation catalyst was found to require 10 mol% of 1a (Scheme 1C) [18].Catalytic application of borane-ammonia achieved fantastic results in direct amidation of various aryl and alkyl carboxylic acids and amines, delivering appreciable yields of a variety of carboxamides.
However, several issues with the use of 1a soon became apparent, especially with the transition to continuous flow synthesis in mind.The static nature of solid 1a makes handling cumbersome.The low solubility of 1a in the reaction solvent additionally makes the potential advancement from batch to continuous flow synthesis difficult.Herein, we report the use of pyridine-borane (5 mol%) as an effective and efficient liquid amidating reagent for the synthesis of various aryl and alkyl carboxamides in good to excellent yields (Scheme 1D).

Results and Discussion
As a part of the project utilizing catalytic 1a, borane-trimethylamine (1i) and triethylamine borane (1j) were tested and provided acceptable yields.The optimization studies carried out yielded model amide 4a in 93% and 91% yields with 1i and 1j respectively.Since 1a produced a higher yield, it was ultimately chosen as the preferred catalyst at that time.
However, several issues with the use of 1a soon became apparent, especially with the transition to continuous flow synthesis in mind.The static nature of solid 1a makes handling cumbersome.The low solubility of 1a in the reaction solvent additionally makes the potential advancement from batch to continuous flow synthesis difficult.Herein, we report the use of pyridine-borane (5 mol%) as an effective and efficient liquid amidating reagent for the synthesis of various aryl and alkyl carboxamides in good to excellent yields (Scheme 1D).

Results and Discussion
As a part of the project utilizing catalytic 1a, borane-trimethylamine (1i) and triethylamine borane (1j) were tested and provided acceptable yields.The optimization studies carried out yielded model amide 4a in 93% and 91% yields with 1i and 1j respectively.Since 1a produced a higher yield, it was ultimately chosen as the preferred catalyst at that time.
To overcome the identified challenges posed by the use of 1a, our focus shifted to exploring alternate borane-amines for direct amidation.Thus, a variety of 1 • (isopropylamine (1b), cyclohexylamine (1c), benzylamine (1d), t-butylamine (1e)), 2 • (dimethylamine (1f), piperidine (1g), 2,2,6,6-tetramethylpiperidine (1h)), 3 • (trimethylamine (1i), triethylamine (1j), N,N-dimethylcyclohexylamine (1k)), and heteroaromatic (pyridine (1l)) borane-amines were examined (Scheme 2).Each of the borane-amines tested provided good to excellent yield of the model amide 4a.However, the use of borane coordinated to 1 • and 2 • amines (1b-1h) was eliminated due to the detection (in the 1 H NMR spectra of the product) of a second byproduct amide formed from the borane-coordinated amine and carboxylic acid.The lower yields obtained when using 1i and 1j, and the drawbacks associated with 1k, including cost and difficulty in separation from the reaction mixture due to high boiling point and low water solubility, led to the selection of borane-pyridine (1l) as the optimal catalyst.Additional benefits of 1l include the fact that it is a liquid, it works at a lower (5 mol%) catalyst loading, and our recently described large-scale, green synthesis of 1l eliminates the use of tetrahydrofuran from its preparation [19].
and 2° amines (1b-1h) was eliminated due to the detection (in the 1 H NMR spectra of the product) of a second byproduct amide formed from the borane-coordinated amine and carboxylic acid.The lower yields obtained when using 1i and 1j, and the drawbacks associated with 1k, including cost and difficulty in separation from the reaction mixture due to high boiling point and low water solubility, led to the selection of borane-pyridine (1l) as the optimal catalyst.Additional benefits of 1l include the fact that it is a liquid, it works at a lower (5 mol%) catalyst loading, and our recently described large-scale, green synthesis of 1l eliminates the use of tetrahydrofuran from its preparation [19].Standardization of conditions led to the use of 1 eq. of amine and 1.1 eq. of carboxylic acid, similar to previous protocol [18].Completion time for the majority of the examples is 12 h, although some required only 6 h.Xylenes proved to be the appropriate solvent for pyridine-borane 1l catalyzed amidation, while a comparatively lower boiling point solvent such as toluene resulted in a decreased yield of 75% of 4a, proving the necessity for higher temperatures.While 5 mol% of 1l is adequate to carry forth the transformations, some substrates require an increased loading of 10 mol% to improve carboxamide yield.Standardization of conditions led to the use of 1 eq. of amine and 1.1 eq. of carboxylic acid, similar to previous protocol [18].Completion time for the majority of the examples is 12 h, although some required only 6 h.Xylenes proved to be the appropriate solvent for pyridine-borane 1l catalyzed amidation, while a comparatively lower boiling point solvent such as toluene resulted in a decreased yield of 75% of 4a, proving the necessity for higher temperatures.While 5 mol% of 1l is adequate to carry forth the transformations, some substrates require an increased loading of 10 mol% to improve carboxamide yield.
Loading of a lower sub-stoichiometric value of 1l was also tested.Decreasing the amount of pyridine-borane 1l from 5 mol% to 1 mol% yielded 4a in 98%, but when similar conditions were analyzed against 4j, only 54% of the product could be isolated.Further decreasing the loading to 0.1 mol% isolated the carboxamide in 69% yield.Since the latter sub-stoichiometric measurements were only effective for one substrate, 5 mol% was determined as the ideal loading for further analysis.A 100 mmol scale was additionally performed for this substrate (4a), where a slightly decreased yield of 67% (14.2 g) was obtained, likely due to a buildup of byproduct water too much for azeotropic removal during the course of the reaction.
The substrate scope analysis for carboxamide synthesis using 1l was performed utilizing a variety of aromatic and aliphatic starting materials (Scheme 3).Aromatic carboxylic acids were initially studied with aliphatic amines.Cyclohexylamine 3b underwent amidation with benzoic acid (2a) to give the product (4b) in 69% yield.In contrast, when 1a was used, the reaction produced carboxamide 4b in 98% yield, proving the previous method to be more efficient for the synthesis of this particular carboxamide [18].
Loading of a lower sub-stoichiometric value of 1l was also tested.Decreasing the amount of pyridine-borane 1l from 5 mol% to 1 mol% yielded 4a in 98%, but when similar conditions were analyzed against 4j, only 54% of the product could be isolated.Further decreasing the loading to 0.1 mol% isolated the carboxamide in 69% yield.Since the latter sub-stoichiometric measurements were only effective for one substrate, 5 mol% was determined as the ideal loading for further analysis.A 100 mmol scale was additionally performed for this substrate (4a), where a slightly decreased yield of 67% (14.2 g) was obtained, likely due to a buildup of byproduct water too much for azeotropic removal during the course of the reaction.
The substrate scope analysis for carboxamide synthesis using 1l was performed utilizing a variety of aromatic and aliphatic starting materials (Scheme 3).Aromatic carboxylic acids were initially studied with aliphatic amines.Cyclohexylamine 3b underwent amidation with benzoic acid (2a) to give the product (4b) in 69% yield.In contrast, when 1a was used, the reaction produced carboxamide 4b in 98% yield, proving the previous method to be more efficient for the synthesis of this particular carboxamide [18].For the tertiary carboxamide 4d, 5 mol% of 1l was insufficient and 10 mol% was used to produce a yield of 84%.However, amidation of the bulky dibenzylamine (3e) with 2a required 50 mol% of 1l to give an isolated yield of 79%, likely due to the hindered nature of the amine.This finding was in agreement with results of 1a [18].Interestingly, when p-nitrobenzoic acid (2b) was reacted with benzylamine (3a), borane-pyridine 1l produced 4f in 80% yield, which in comparison only yielded 69% when 1a was used [18], showing the present protocol to be superior for carboxylic acid substrates bearing electronwithdrawing substituents.Cinnamic acid (2c) showed excellent reactivity with amines 3a and 3b, hexylamine (3c), and morpholine (3d) with quantitative yields of 4g, 4h, 4i, and 4j in the presence of 5 mol% 1l.
In the amidation scope of carboxamides bearing an aromatic acid and an aromatic amine, aniline (3f) yielded 68% in 12 h when it reacted with 2a.On increasing the loading of 1l to 10 mol%, the carboxamide 4k was isolated in a similar yield after 6 h of reflux in xylenes.Reflux for 12 h resulted in decomposition.Keeping the aromatic acid (2a) as constant, p-anisidine (3g) yielded the corresponding amide (4l) in 73% yield with 5 mol% 1l and similar results were obtained when testing 10 mol%.o-Toluic acid (2d) reacted with 3f and 3g to produce 41% and 87% yields of carboxamides 4m and 4n respectively.However, when 2d was treated with 3f using 10 mol% of 1l, the yield increased to 54%.Cinnamic acid (2c) and aniline (3f) provided the isolated product 4o at 95% yield using 5 mol% of 1l.
Mechanistically, we propose the formation of a key intermediate triacyloxyboraneamine complex to occur through successive dehydrogenative addition of the carboxylic acid to pyridine borane, closely related to our earlier reports on borane-amine promoted carboxamide synthesis [18].This intermediate then undergoes nucleophilic attack by the reactant amine to form the desired carboxamide.
To determine the selectivity of the carboxamide formation versus the reaction of the triacyloxyborane-amine complex with other potential nucleophiles, a reaction with a competing esterification was performed (Scheme 4).A baseline esterification between phenylacetic acid (2e) and benzyl alcohol (BnOH) provided the ester benzyl phenylacetate in nearly quantitative yield after 12 h in refluxing toluene, despite the lack of any added catalyst.Following this baseline esterification, a reaction with a competing amidation and esterification was performed.Equimolar quantities of phenylacetic acid (2e), benzyl alcohol (BnOH), and benzylamine (3a), along with 5 mol% of borane-pyridine (1l) were heated in refluxing xylenes for 12 h.The resulting reaction mixture was analyzed using 1 H NMR spectroscopy where a ratio of 48:2:50 of the unreacted benzyl alcohol to the benzyl phenylacetate ester to the amide 4s was observed, indicating a very high degree of selectivity towards the amidation versus the competing esterification.Further details can be found in the Supplementary Materials.

General Information
Unless otherwise noted, all additions were carried out under open air conditions. 11B, 13 C, and 1 H NMR spectra were recorded at room temperature, on a Bruker 300 MHz NMR spectrophotometer.Chemical shifts (δ values) are reported in parts per million relative to BF3.Et2O for 11 B NMR, respectively.Data are reported as: δ value, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad), and integration.All solvents for routine isolation of products were reagent-grade.Amines, carboxylic acids, and sodium borohydride were purchased from Sigma-Aldrich and/or Oakwood Chemicals and used without further purification.All borane-amines used for optimization studies were prepared using the reported procedure [20].All reactions requiring heat were brought to temperature using an oil bath and heated stir plate.

Experimental
Procedure for the preparation of borane-pyridine [19] consisted of sodium borohydride (2 eq, 0.2 mole, 7.566 g) being charged to a 200 mL round-bottom flask containing a stir-bar followed by the addition of 100 mL ethyl acetate (1 M w.r.t.amine).With stirring, pyridine (1 eq, 0.1 mole, 8.05 mL) was added with the help of a syringe and, subsequently, 15 mL of water was added in three intervals (vigorous stirring required).After completion of the reaction at 20 h, the reaction mixture was transferred to a 500 mL separatory funnel using 100 mL ethyl acetate and 50 mL water.The organic layer was subjected to two additional washes with 50mL portions of water, then 50 mL of brine, and dried over sodium sulfate.The mixture was then filtered through cotton and condensed via rotary evaporation.The condensed liquid was passed through celite in a cotton-plugged pipette and washed with small ~5 mL portion of dichloromethane.The resulting colorless liquid was stirred under high vacuum to remove any remaining solvent and used without further purification.Characterization data for borane-pyridine can be found in the Supplementary Materials.

General Information
Unless otherwise noted, all additions were carried out under open air conditions. 11B, 13 C, and 1 H NMR spectra were recorded at room temperature, on a Bruker 300 MHz NMR spectrophotometer.Chemical shifts (δ values) are reported in parts per million relative to BF 3 .Et 2 O for 11 B NMR, respectively.Data are reported as: δ value, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad), and integration.All solvents for routine isolation of products were reagent-grade.Amines, carboxylic acids, and sodium borohydride were purchased from Sigma-Aldrich and/or Oakwood Chemicals and used without further purification.All borane-amines used for optimization studies were prepared using the reported procedure [20].All reactions requiring heat were brought to temperature using an oil bath and heated stir plate.

Experimental
Procedure for the preparation of borane-pyridine [19] consisted of sodium borohydride (2 eq, 0.2 mole, 7.566 g) being charged to a 200 mL round-bottom flask containing a stir-bar followed by the addition of 100 mL ethyl acetate (1 M w.r.t.amine).With stirring, pyridine (1 eq, 0.1 mole, 8.05 mL) was added with the help of a syringe and, subsequently, 15 mL of water was added in three intervals (vigorous stirring required).After completion of the reaction at 20 h, the reaction mixture was transferred to a 500 mL separatory funnel using 100 mL ethyl acetate and 50 mL water.The organic layer was subjected to two additional washes with 50mL portions of water, then 50 mL of brine, and dried over sodium sulfate.The mixture was then filtered through cotton and condensed via rotary evaporation.The condensed liquid was passed through celite in a cotton-plugged pipette and washed with small ~5 mL portion of dichloromethane.The resulting colorless liquid was stirred under high vacuum to remove any remaining solvent and used without further purification.Characterization data for borane-pyridine can be found in the Supplementary Materials.
General procedure for the preparation of amides consisted of a 100 mL round bottom flask containing a magnetic stir-bar being charged with carboxylic acid (5.5 mmol, 1.1 eq.) and xylenes (5 mL, 1 M with respect to amine).To this stirring mixture, pyridine-borane ((0.25 mmol, 0.05 eq.) or (0.5 mmol, 0.1 eq.) or (2.5 mmol, 0.5 eq.)) was introduced followed by the addition of the respective amine (5 mmol, 1 eq.).A reflux condenser was attached to the flask and the reaction mixture was brought to reflux using an oil bath.After completion

Scheme 2 .
Scheme 2. Scope of examined borane-amine catalysts.a Isolated yields are shown.b Yield obtained when using 5 mol%.

Scheme 2 .
Scheme 2. Scope of examined borane-amine catalysts.a Isolated yields are shown.b Yield obtained when using 5 mol%.