The Tryptophan Metabolite Indole-3-Carboxaldehyde Alleviates Mice with DSS-Induced Ulcerative Colitis by Balancing Amino Acid Metabolism, Inhibiting Intestinal Inflammation, and Improving Intestinal Barrier Function

Ulcerative colitis (UC) has attracted much attention for its negative influence on quality of life and increased risk of colorectal cancer. Chemical and biological drugs are currently the usual treatment for UC. These drugs always induce severe side effects, or patients might become resistant to these therapies. Therefore, new therapeutic options for UC are urgently needed. In this study, we discovered the inhibitory activity of the intestinal tryptophan metabolite indole-3-carboxaldehyde (3-IAld) in dextran sulfate sodium salt (DSS)-induced UC mice by targeting the TLR4/NF-κB/p38 signaling pathway. This compound effectively protected against colon length shortening and damage induced by DSS in the colon, notably reducing the severity of inflammation. The production of inflammatory factors of TNF-α, IL-6, and IL-1β was significantly attenuated when treating with 3-IAld in vivo and vitro. This might be attributed to inhibition of the TLR4/NF-kB/p38 signaling pathway. Moreover, 3-IAld could up-regulate the expression of ZO-1 and Occludin in vivo and vitro. Meanwhile, liquid chromatography mass spectrometry (LC-MS) results showed that 3-IAld could balance the aspartate and glutamate metabolism and the lysine degradation metabolism in the serum of DSS-induced colitis mice. In conclusion, 3-IAld ameliorated the intestinal barrier dysfunction and inflammatory response in DSS-induced UC mice, balanced amino acid metabolism, and inhibited the activation of the TLR4/NF-kB/p38 signaling pathway, thereby protecting mice with colitis.


Introduction
Inflammatory bowel disease (IBD), including the two main subtypes of Crohn's disease (CD) and ulcerative colitis (UC), is an inflammatory disorder of the intestinal tract [1]. UC is characterized by intestinal barrier dysfunction, tissue damage, and inflammatory cell infiltration. Immunosuppressive agents, amino salicylates, and corticosteroids are the main drugs used to treat UC in modern clinical medicine [2]. Although they can alleviate the clinical symptoms of patients to a certain extent, the cure rate is low, and the treatment cost is high and often leads to a variety of adverse reactions. Therefore, there is an urgent need to develop targeted, effective, and non-toxic drugs for clinical treatment of UC.
Accumulating evidence suggests that UC is closely related to the homeostasis of the intestinal epithelium [3]. A complete intestinal barrier and intestinal mucosal immunity are the prerequisites for maintaining intestinal health. Intestinal barrier function is maintained by tight junction (TJs) proteins and other molecular complexes [4]. TJs, which include claudins, Occludin, and zonula occludens-1 (ZO-1), are highly dynamic complexes that seal the space among adjacent epithelial cells and are involved in regulating the permeability of substances in the gut [5]. Defects in TJ integrity eventually lead to intestinal barrier dysfunction [6]. The invasion of pathogens or other harmful substances (such as LPS, Lipopolysaccharide) will lead to the release of a series of inflammatory cytokines and over-production of reactive oxygen species (ROS) in the intestinal tract [7]. Therefore, inhibiting inflammatory cytokines and excessive oxidative stress efficiently ameliorates UC. The toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway plays a critical role in the inflammatory response, especially the activation of macrophages in the intestinal tissue [8]. TLR4, the first TLR protein discovered, is associated with a variety of inflammatory responses and is a major receptor for lipopolysaccharide responses [9]. The interaction between TLR4 and Myeloid differentiation factor 88 (MyD88) in the TIR domain triggers a cascade of downstream signals that activate the NF-κB signaling pathway, secreting a variety of inflammatory cytokines and ultimately participating in the inflammatory response. Studies have shown that LPS binding to TLR4 leads to the activation of transcription factors, including NF-κB and mitogen-activated protein kinase p38 (MAPK p38) [10][11][12]. Activation of transcription factors leads to the production of several pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6).
Activation of aryl hydrocarbon receptors (AhR) in the intestine promotes the secretion of IL-22 by intestinal epithelial cells and the growth of epithelial cells in the intestine, thus maintaining intestinal health [13]. Metabolite 3-Indolealdehyde (3-IAld) is a common tryptophan metabolite in the intestinal tract and has significant anti-inflammatory effects by activating AhR receptors, such as reducing chondrocyte inflammation by modulating the AhR-NF-κB signaling pathway [14], and it has also been shown to restore the integrity of the intestinal mucosa [15]. However, the exact mechanism by which 3-IAld treats UC remains unclear. In this study, we evaluated its anti-inflammatory and antioxidant activities in vitro. In vivo, we determined the anti-inflammatory effect of the compound on DSS-induced UC in C57BL/6 mice. Furthermore, metabolic disorders occur in patients with colitis, and many researchers have elucidated the potential targets and mechanisms of many drugs in the treatment of specific diseases with metabolomics strategies successfully. Therefore, in this study we used the liquid chromatography mass spectrometry (LC-MS) technique to investigate the effect of 3-IAld on the serum of DSS-induced colitis mice.
The results showed that 3-Iald could significantly repair intestinal barrier damage in UC mice and inhibit the expression of multiple inflammatory factors by inhibiting the activation of the TLR4/NF-κB/MAPK p38 signaling pathway. In addition, LC-MS results showed that 3-Iald was able to intervene in colitis mice by balancing amino acid metabolism. In conclusion, 3-Iald is expected to be a potentially effective drug for the treatment of UC.

3-Iald Inhibited the Expression Levels of NO, TNF-α, IL-6, and IL-1β Induced by LPS in RAW264.7 Cells
In order to investigate the anti-inflammatory activity of 3-Iald, the expression levels of NO, TNF-α, IL-6, and IL-1β were measured by ELISA in LPS-induced RAW264.7 cells. The results showed that LPS significantly increased the levels of NO, TNF-α, IL-6, and IL-1β in the supernatant of RAW264.7 cells compared with the normal group. Compared to the LPS group, 3-Iald co-treatment reduced the expression level of NO, and the concentrations of 20 and 40 µM have a significant difference (Figure 2b). The 3-Iald co-treatment dose-dependently reduced the expression levels of TNF-α, IL-6, and IL-1β in the supernatant (Figure 2b  (c) Caco2 was co-treated with 3-Iald (0-160 µM) for 24 h, and cell viability was assessed by CCK-8 assay. Data were expressed as means ± SD (n = 6); * p < 0.05, ** p < 0.01 compared with 0 group.

3-Iald
Inhibited the Expression Levels of NO, TNF-α, IL-6, and IL-1β Induced by LPS in RAW264.7 Cells In order to investigate the anti-inflammatory activity of 3-Iald, the expression levels of NO, TNF-α, IL-6, and IL-1β were measured by ELISA in LPS-induced RAW264.7 cells. The results showed that LPS significantly increased the levels of NO, TNF-α, IL-6, and IL-1β in the supernatant of RAW264.7 cells compared with the normal group. Compared to the LPS group, 3-Iald co-treatment reduced the expression level of NO, and the concentrations of 20 and 40 µM have a significant difference (Figure 2b). The 3-Iald co-treatment dosedependently reduced the expression levels of TNF-α, IL-6, and IL-1β in the supernatant (Figure 2b-d).

3-Iald Reduced LPS Induced ROS Production and Up-Regulated the Expression of ZO-1 and Occludin
As shown in Figure 3a, compared with the normal group, the expression levels of ROS in the LPS group were significantly increased. However, 3-Iald co-treatment dosedependently (10, 20, and 40 µM) reduced ROS levels. It is noteworthy that LPS could significantly down-regulate the expression of ZO-1 and Occludin in Caco2 cells. Compared with the LPS group, 3-Iald at 20 and 40 µM concentrations could significantly up-regulate the expression of ZO-1 and Occludin (Figure 3b).  Data were expressed as means ± SD (n = 6); ### p < 0.001, #### p < 0.0001, compared with control group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with LPS group.

3-Iald Reduced LPS Induced ROS Production and Up-Regulated the Expression of ZO-1 and Occludin
As shown in Figure 3a, compared with the normal group, the expression levels of ROS in the LPS group were significantly increased. However, 3-Iald co-treatment dosedependently (10, 20, and 40 µM) reduced ROS levels. It is noteworthy that LPS could significantly down-regulate the expression of ZO-1 and Occludin in Caco2 cells.   Occludin (d) were analyzed by Image J. Data were expressed as means ± SD (n = 3); # p < 0.05, ## p < 0.01, compared with 0 group; * p < 0.05, ** p < 0.01, compared with LPS group.

3-Iald Improved the Shortening of Colon Length and Pathological Damage Caused by DSS in Mice
The UC mouse model was induced by free drinking of 3% DSS for seven days. As shown in Figure 4a,b, the colon length of mice was measured after sacrifice, and the colon length of the normal group was 6.77 cm, the colon length of the model group was 5.39 cm, and the colon length of 3-Iald (10, 20, and 40 mg/kg) administered mice was 6.36, 6.53, and 8.03 cm, respectively. The colons of all three 3-Iald-treated groups were significantly longer than those of the model group. Histological findings were evaluated at the end of the experiment. The results showed that the mucosal structure of mice in the model group was significantly damaged, with massive infiltration of neutrophils and almost complete loss of cupped cells. DSS-induced mucosal lesions and neutrophil infiltration were partially attenuated by moderate-or high-dose 3-IAld as assessed by microscopy, particularly in the 40 mg/kg administration group.

3-Iald Improved the Shortening of Colon Length and Pathological Damage Caused by DSS in Mice
The UC mouse model was induced by free drinking of 3% DSS for seven days. As shown in Figure 4a,b, the colon length of mice was measured after sacrifice, and the colon length of the normal group was 6.77 cm, the colon length of the model group was 5.39 cm, and the colon length of 3-Iald (10, 20, and 40 mg/kg) administered mice was 6.36, 6.53, and 8.03 cm, respectively. The colons of all three 3-Iald-treated groups were significantly longer than those of the model group. Histological findings were evaluated at the end of the experiment. The results showed that the mucosal structure of mice in the model group was significantly damaged, with massive infiltration of neutrophils and almost complete loss of cupped cells. DSS-induced mucosal lesions and neutrophil infiltration were partially attenuated by moderate-or high-dose 3-IAld as assessed by microscopy, particularly in the 40 mg/kg administration group.  Data were expressed as means ± SD (n = 6); # p < 0.05, compared with normal group; * p < 0.05, ** p < 0.01, compared with model group.

3-IAld Alleviated the Disruption of the Colonic Barrier Caused by DSS in Mice
In colon tissue, the structural integrity of the intestinal barrier is the basis for maintaining the normal function of the intestine. The expressions of ZO-1 and Occludin proteins were examined by western blotting. The results showed that the expression levels of ZO-1 and Occludin in colon tissues were significantly down-regulated in the model group compared with the normal group, which indicated that DSS could disrupt the barrier function of colon tissues. Compared with the model group, administration of 3-IAld (20 mg/kg) significantly improved the expression level of ZO-1 in colon tissue, but the expression level of Occludin could not be up-regulated. Administration of 3-IAld (40 mg/kg) significantly improved the expression levels of ZO-1 and Occludin (Figure 5a,b and d). The immunohistochemical results showed a significant reduction in the expression of ZO-1 and Occludin in the model group compared to the normal group. The expression of these proteins was reversed to varying degrees in each administration group compared to the model group (Figure 5d,e).
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3-IAld Alleviated the Disruption of the Colonic Barrier Caused by DSS in Mice
In colon tissue, the structural integrity of the intestinal barrier is the basis for maintaining the normal function of the intestine. The expressions of ZO-1 and Occludin proteins were examined by western blotting. The results showed that the expression levels of ZO-1 and Occludin in colon tissues were significantly down-regulated in the model group compared with the normal group, which indicated that DSS could disrupt the barrier function of colon tissues. Compared with the model group, administration of 3-IAld (20 mg/kg) significantly improved the expression level of ZO-1 in colon tissue, but the expression level of Occludin could not be up-regulated. Administration of 3-IAld (40 mg/kg) significantly improved the expression levels of ZO-1 and Occludin (Figure 5a,b and d).
The immunohistochemical results showed a significant reduction in the expression of ZO-1 and Occludin in the model group compared to the normal group. The expression of these proteins was reversed to varying degrees in each administration group compared to the model group (Figure 5d,e). Occludin protein expression in the colonic tissue of UC mice. Data were expressed as means ± SD (n = 3); ## p < 0.01, compared with normal group; ** p < 0.01, *** p < 0.001, compared with model group.

3-IAld Inhibited the Expression Levels of TNF-α, IL-6, and IL-1β, and Down-Regulated the Expression Levels of TLR4, P-NF-κB, and P-P38 Proteins in DSS-Induced UC Mice
As shown in Figure 6a-c, the levels of TNF-α, IL-6, and IL-1β in the serum of mice in the model group were significantly increased compared with the normal group, while 3-IAld administration groups inhibited the levels of TNF-α, IL-6, and IL-1β in the serum to different degrees compared with the model group. Elevated expression of inflammationassociated proteins in colon tissues is one of the intrinsic features of UC. As expected, overexpressed protein expression was observed in DSS-treated mice. Administration with 3-IAld significantly reduced the protein levels of TLR4, P-NF-κB, and P-p38 in the colon tissues of mice.  As shown in Figure 6a-c, the levels of TNF-α, IL-6, and IL-1β in the serum of mice in the model group were significantly increased compared with the normal group, while 3-IAld administration groups inhibited the levels of TNF-α, IL-6, and IL-1β in the serum to different degrees compared with the model group. Elevated expression of inflammationassociated proteins in colon tissues is one of the intrinsic features of UC. As expected, overexpressed protein expression was observed in DSS-treated mice. Administration with 3-IAld significantly reduced the protein levels of TLR4, P-NF-κB, and P-p38 in the colon tissues of mice. The levels of (a) TNF-α, (b) IL-6, and (c) IL-1β in serum of mice were measured using ELISA kits. (d) Western blotting technique was used to detect the protein expression levels of TLR4, P-NF-κB, NF-κB, p38, and P-p38 in the colonic tissues of UC mice. The relative expressions of (e) TLR4, (f) P-NF-κB/NF-κB, and (g) P-p38/p38 were analyzed by Image J. Data were expressed as means ± SD (n = 3); # p < 0.05, ### p < 0.001, compared with normal group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with model group.

Effect of 3-IAld on Serum Metabolism in DSS-Induced C57BL/6 Mice
To further investigate the effect of 3-IAld on the serum metabolism of DSS-induced colitis mice, we selected the 3-IAld medium-dose group (20 mg/kg) and performed an LC-MS assay on the metabolites in their serum. As shown in Figure 7a,b, the results of PCA treatment in both positive and negative ion modes showed a significant separation trend in the normal, model, and 3-IAld groups, in addition to the results of OPLS-DA treatment, which also showed a significant separation trend in the normal, model, and 3-IAld groups, indicating that 3-IAld could significantly affect the serum metabolism of DSS-induced colitis mice. The results of the orthogonal experiments showed that the metabolic data in this case were stable and reliable (Figure 7e,f). Next, we screened the compounds in each group according to (VIP > 1, p < 0.05); as shown in Figure 7g, 3 compounds were significantly lower and 10 were significantly higher in the model group compared with the normal group, and these 13 compounds were significantly rolled back after administration. Please see Table 1 for compound-specific information. Next, these 13 metabolic differences were substituted into the MetaboAnalyst database for metabolic pathway enrichment analysis, which showed that 3-IAld was able to affect alanine, aspartate, and glutamate metabolism and the lysine degradation metabolism pathway in the serum of DSS-induced Figure 6. Effect of 3-IAld on the expression levels of inflammatory factors in serum and inflammationrelated protein expression in intestinal tissues of UC mice. The levels of (a) TNF-α, (b) IL-6, and (c) IL-1β in serum of mice were measured using ELISA kits. (d) Western blotting technique was used to detect the protein expression levels of TLR4, P-NF-κB, NF-κB, p38, and P-p38 in the colonic tissues of UC mice. The relative expressions of (e) TLR4, (f) P-NF-κB/NF-κB, and (g) P-p38/p38 were analyzed by Image J. Data were expressed as means ± SD (n = 3); # p < 0.05, ### p < 0.001, compared with normal group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with model group.

Effect of 3-IAld on Serum Metabolism in DSS-Induced C57BL/6 Mice
To further investigate the effect of 3-IAld on the serum metabolism of DSS-induced colitis mice, we selected the 3-IAld medium-dose group (20 mg/kg) and performed an LC-MS assay on the metabolites in their serum. As shown in Figure 7a,b, the results of PCA treatment in both positive and negative ion modes showed a significant separation trend in the normal, model, and 3-IAld groups, in addition to the results of OPLS-DA treatment, which also showed a significant separation trend in the normal, model, and 3-IAld groups, indicating that 3-IAld could significantly affect the serum metabolism of DSS-induced colitis mice. The results of the orthogonal experiments showed that the metabolic data in this case were stable and reliable (Figure 7e,f). Next, we screened the compounds in each group according to (VIP > 1, p < 0.05); as shown in Figure 7g, 3 compounds were significantly lower and 10 were significantly higher in the model group compared with the normal group, and these 13 compounds were significantly rolled back after administration. Please see Table 1 for compound-specific information. Next, these 13 metabolic differences were substituted into the MetaboAnalyst database for metabolic pathway enrichment analysis, which showed that 3-IAld was able to affect alanine, aspartate, and glutamate metabolism and the lysine degradation metabolism pathway in the serum of DSS-induced colitis mice.  Data were expressed as means ± SD (n = 6); # p < 0.05, ## p < 0.01, compared with normal group; * p < 0.05, ** p < 0.01, **** p < 0.0001, compared with model group.

Discussion
UC is an idiopathic IBD affecting the gastrointestinal tract with lesions mainly in the colon, rectum, or the entire colorectal region. In this study, LPS-induced RAW264.7 cells, Caco2 cells, and DSS-induced colitis mice models were used to investigate the protective effects and mechanisms of specific tryptophan metabolites (3-IAld) produced by intestinal microbes on mice with colitis. The results of vitro experiments showed that 3-IAld effectively reduced LPS-induced inflammation in RAW264.7 cells and increased the protein levels of ZO-1 and Occludin in Caco2 cells pretreated by LPS. Vivo results showed that 3-IAld significantly improved the intestinal pathological damage in DSS-induced colitis mice; reduced serum inflammatory cytokines including TNF-α, IL-6, and IL-1β; improved intestinal barrier function; inhibited the activation of the TLR4/NF-κB/p38 signaling pathway in the intestine; and balanced amino acid metabolism in the serum of mice, thereby protecting mice from colitis injury.
The symptoms of UC are manifested by overproduction of inflammatory cytokines. In the study, we found that 3-IAld was able to inhibit the release of TNF-α, IL-6, and IL-1β in vivo and in vitro. These results indicate that 3-IAld has good anti-inflammatory activity. It is well known that intercellular TJs are an extremely important component of the epithelial structure that forms the intestinal barrier [3]. This barrier structure usually prevents toxic substances, such as LPS, from penetrating the intestinal cavity [16]. To date, the exact mechanism by which epithelial permeability changes has not been understood. Multiple inflammatory cytokines (such as TNF-a, IL-6, and IL-1β) secreted in the intestinal tract of UC patients may be the main cause of TJ destruction [17]. TJs play an important role in maintaining the integrity of the intestinal mucosal barrier, which is one of the strategies for the treatment of UC [18]. Treatment with 3-IAld significantly elevated the expression of Occludin and ZO-1 proteins. As we expected, these results are consistent with in vitro results. By increasing ZO-1 and Occludin proteins in the intestine of UC mice by 3-IAld, on the one hand, and by decreasing the release of inflammatory factors in the intestine, on the other hand, 3-IAld has been shown to activate the AhR receptor, which also plays a positive role in the repair of the intestinal barrier [19,20]. The TLR4 signaling pathway is one of the important mechanisms of inflammation response and a key co-recognition receptor for intestinal innate immunity [21]. The TLR4 signaling pathway is the subject of therapy (targeted inhibition) for UC [22][23][24]. In addition, NF-κB and P38 can also be activated by TLR4 to aggravate UC symptoms [10,25]. NF-κB is a nuclear transcription factor regulating overproduction of various inflammatory cytokines and adhesion molecules in a wide range of cells, thereby participating in the inflammatory reaction process. It controls the transcriptional activity of several pro-inflammatory cytokine promoters, as well as transcription factors involved in intestinal inflammation [8]. In addition, mitogen-activated protein kinase (MAPK) signaling, including p38 MAPK, plays a vital role in the modulation of inflammatory cytokines. In our experiments, we found that 3-IAld was able to reduce the expression levels of TLR4, P-NF-κB, and P-p38 proteins in the colonic tissue of UC mice, thereby inhibiting colonic inflammation, which is consistent with previous studies.
In addition, during the progression of UC, host metabolic pathways change in response to stimulation by multiple pro-inflammatory cytokines and pathogens and their metabolites, and these changes further exacerbate impaired intestinal function, leading to irreversible damage. More importantly, the onset and progression of UC are closely associated with abnormal amino acid metabolism, and amino acid supplementation can reduce inflammation and oxidative stress in inflammatory bowel disease, whereas dietary amino acid deficiency exacerbates the severity of DSS-induced colitis [25]. In the present study, 3-IAld was found to balance alanine, aspartate, and glutamate metabolism and lysine degradation in the serum of mice with DSS-induced colitis, indicating that 3-IAld can improve the symptoms of DSS-induced colitis in mice by affecting amino acid metabolism. However, this study has not yet elucidated the targets of 3-IAld in the treatment of ulcerative colitis, and its clinical trial safety and pharmacokinetic characteristics still require further in-depth research.

Cell Viability Assessment
RAW264.7 cells and Caco2 cells were seeded into 96-well plates at a density of 10,000 cells/well and 2000 cells/well, respectively, and placed in the cell culture incubator overnight. The 3-IAld was added and incubated with cells for 24 h, then CCK-8 (100 µL) was added into each well for another 30-60 min. Finally, the OD value was measured by microplate reader (Bio Tek, Winooski, VT, USA) at 450 nm, and the cell viability (%) was calculated.

Enzyme-Linked Immunosorbent Assay (ELISA)
Cells were seeded into six-well plates and incubated with different concentrations of 3-IAld for 2 h before being treated with LPS for 24 h. After treatment, the supernatant was collected and centrifugated, and the level of TNF-α, IL-6, and IL-1β was measured by using enzyme-linked immunosorbent assay (ELISA) kits (Beyotime, Shanghai, China) according to the manufacturer's instructions. The level of TNF-α, IL-6, and IL-1β in the serum of mice was also detected using ELISA kits.

Intracellular ROS Assay
Caco2 cells were seeded and incubated with different concentrations of 3-IAld for 2 h before LPS (2 µg/mL) was added for 24 h. According to the manufacturer's instructions, the cells were stained with the fluorescent marker 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) (10 µM; Sigma-Aldrich, St. Louis, MO, USA). Then the fluorescence intensity was detected by fluorescence microscopy (Olympus Corporation, Tokyo, Japan), and the level of ROS production was determined by the fluorescence level.

Western Blotting Assay
Western blotting assay was used to separate the lysate from isolated sterile colonic tissues or cells. In brief, the lysates were separated using 12% SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and transferred onto a polyvinylidene fluoride membrane (PVDF). Furthermore, the PVDF membrane was blocked with 5% non-fat dry milk for 1 h at room temperature and probed with primary antibodies at 4 • C overnight. Subsequently, the membrane was incubated with HRP-secondary antibody for 1 h at 37 • C. Finally, blot bands were visualized using enhanced chemiluminescence (ECL; Beyotime, Shanghai, China) reagent and captured by an imager (Chemi Scope6200; Clinx Science Instruments Co., Ltd., Shanghai, China). Finally, Image J software was used to calculate the gray value for quantitative analysis.

DSS Mouse Models
Male C57BL/6 mice (6-8 weeks old; weighing 19-22 g) were purchased from the National Institutes for Food and Drug Control (License Number: SCXK (Beijing) 2019-0006). Mice were housed in an air-conditioned room with the laboratory temperature maintained at 25 ± 2 • C, relative humidity of 50 ± 5%, 12 h of light/dark cycle, and access to food and water randomly. All animal experimental procedures were performed in accordance with the Guide and Use of Laboratory Animals and approved by the Laboratory Animal Ethics Committee of Lunan Pharmaceutical Group Ltd. DSS (molecular weight at 36-50 kDa) (MP Biomedicals, Morgan Irvine, CA, USA) was dissolved at 3.0% w/v with distilled water. The UC mouse model was induced by DSS for seven days followed by another three days with fresh water. Mice were given pure water and 3-IAld by gavage, and this continued until the end of the experiment. Mice were sacrificed on day 10. The colon tissues were collected and fixed in 4% paraformaldehyde solution for pathological detection for further study.

Hematoxylin and Eosin (H&E) Staining
The colon tissues embedded in paraffin were sliced into 3-5 µm thick sections and dehydrated via graduated ethanol series. Sections were stained with H&E. The level of inflammation and tissue damage was observed by three pathologists.

Immunohistochemistry (IHC)
After gradient dewaxing with xylene and ethanol, sections of colon tissue were heated in sodium citrate solution. Sections were covered with 3% hydrogen peroxide to extinguish endogenous peroxidase. PBS was then used to flush the sections three times, with the addition of 5% normal goat serum to block nonspecific binding. Next, sections were incubated with primary antibody (1:250) at 4 • C overnight. After washing with PBS three times, sections were incubated with biotin-labeled secondary antibody and streptavidinhorseradish peroxidase at room temperature, and then diaminobenzidine (DAB; Beijing Heliogod Technology Co., Ltd., Beijing, China) was added. After staining, the sections were dehydrated and sealed with a neutral resin. Target proteins were randomly observed under the microscope.

Analysis of The Effect of 3-IAld on Serum Metabolism in UC Mice Using the Non-Targeted Metabolomics Technique
The non-targeted metabolomics technique was used to analyze the endogenous metabolites of mice feces. In brief, a 60 mg fecal sample of each mouse was prepared, and an ice-cold mixture of methanol and water (4:1 v/v) was added. After freezing at −20 • C for 5 min, the samples were ground at 60 HZ for 2 min, and then quickly transferred to the ice bath for ultrasonic treatment for 10 min. Subsequently, fecal samples were stored at −20 • C for 30 min. The samples were centrifuged at 13,000 rpm at 4 • C for 15 min, and then a 400 µL mixture of methanol and water (1:4, v/v) was added to each sample, followed by being vortexed for 30 s and then placed at −20 • C for 2 h. The samples were centrifuged at 13,000 rpm at 4 • C for 10 min, and 150 µL of each sample was collected and filtered through a 0.22 µm microfilter to remove impurities. A Dionex Ultimate 3000 RS UHPLC system fitted with a Q Exactive quadrupole Orbitrap mass spectrometer equipped with a heated electrospray ionization (ESI) source (Thermo Fisher Scientific, Waltham, MA, USA) was used to analyze the metabolic profile.

Statistical Analysis
All data were represented as the mean ± SD (standard deviation). Comparisons between the different groups were analyzed by one-way analysis of variance (ANOVA), and a multiple comparison test was performed using the post hoc Bonferroni correction. p < 0.05 was considered statistically significant.

Conclusions
In summary, 3-Iald improves UC mice symptoms by reducing TLR4 expression, inhibiting NF-κB and p38 activation, attenuating histopathological changes, decreasing the expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), increasing the expression of TJs (ZO-1 and Occludin), and balancing amino acid metabolism. Therefore, this study demonstrates that not only the reduction of inflammation and the repair of intestinal barrier are the basis for the treatment of UC, but also that the improvement of amino acid metabolic disturbance is the key to its enhanced therapeutic effect.