Effect of Poly(Vinyl Alcohol) Concentration and Chain Length on Polymer Nanogel Formation in Aqueous Dispersion Polymerization

Nanotechnology has attracted increasing interest in various research fields for fabricating functional nanomaterials. In this study, we investigated the effect of poly(vinyl alcohol) (PVA) addition on the formation and thermoresponsive properties of poly(N-isopropyl acrylamide)-based nanogels in aqueous dispersion polymerizations. During dispersion polymerization, PVA appears to play three roles: (i) it bridges the generated polymer chains during polymerization, (ii) it stabilizes the formed polymer nanogels, and (iii) it regulates the thermoresponsive properties of the polymer nanogels. By regulating the bridging effect of PVA via changing the PVA concentration and chain length, the size of the obtained polymer gel particles was maintained in the nanometer range. Furthermore, we found that the clouding-point temperature increased when using low-molecular weight PVA. We believe that the knowledge gained in this study regarding the effect of PVA concentration and chain length on nanogel formation will aid in the future fabrication of functional polymer nanogels.

Several researchers have investigated the effect of stabilizers on the particle size and thermoresponsive properties of polymer microgels in the dispersion polymerizations. Among a wide range of stabilizers, poly(vinyl alcohol) (PVA) is a representative biocompatible water-soluble stabilizer in the dispersion polymerizations. In the presence of PVA, the radical chain transfer reaction occurs, and the mid-chain radicals generated on PVA initiate the propagation reaction with monomer species, resulting in covalent grafting of PVA on the polymer gel particles [29]. The grafted PVA chain stabilized polymer particles via steric repulsion. To date, the effect of PVA on the synthesis of poly(NIPAm)-based microgels in dispersion polymerizations has been investigated [30,31]. Yates et al. reported the synthesis of PVA-stabilized PNIPAm-based microgels [30]. Guan and Zhang reported that the addition of PVA significantly affected the thermoresponsive properties of phenylboronic acid-functionalized PNIPAm-based microgels [31]. Furthermore, they investigated the effect of PVA molecular weights on the thermoresponsiveness of the PNIPAm-based microgels.
Nanometer-sized polymer gels (nanogels) with a particle size <100 nm are widely used in drug delivery systems for cancer treatments because of their passive targeting properties derived from enhanced permeability and retention effects [32]. However, PNIPAm-based gels generally attain (sub)micrometer-sized particles in precipitation polymerizations. Nanogels have been successfully synthesized via dispersion polymerization using sodium dodecyl sulfate (SDS) as a surfactant [33]. However, SDS is harmful for biological applications. Recently, we have successfully demonstrated the synthesis of PNIPAm-based nanogels via precipitation polymerization (without surfactants) under suitable conditions [34][35][36][37]. Furthermore, the polymer nanogels can recognize intrinsic dysopsonic proteins (serum albumin) and be employed as novel nanocarriers in drug delivery systems [34,38,39]. These polymer nanogels gain stealth capability in situ in the blood vessel by cloaking themselves with intrinsic serum albumin, resulting in their prolonged circulation in the blood [34]. Furthermore, gold nanoparticle-incorporated polymer nanogels prepared via precipitation polymerization have been successfully used as radiation sensitizers [40]. Therefore, the polymer nanogels prepared by precipitation polymerization have been successfully used as drug delivery carriers.
To the best of our knowledge, no studies examining the effect of PVA addition on polymer nanogel formation have been reported. Herein, we investigated the effect of PVA concentration and chain length on the particle formation and thermoresponsive properties of PNIPAm-based nanogels prepared via aqueous dispersion polymerization (Scheme 1). In a series of experiments, we discovered that PVA plays three roles: (i) it bridges the generated polymer chains during polymerization, (ii) it stabilizes the formed polymer nanogels, and (iii) it regulates the thermoresponsive properties of the polymer nanogels when PVA possessing a low molecular weight (degree of polymerization: 500) is used. We believe that the knowledge gained in this study will aid in the future fabrication of functional polymer nanogels.
initiate the propagation reaction with monomer species, resulting in covalent grafting of PVA on the polymer gel particles [29]. The grafted PVA chain stabilized polymer particles via steric repulsion. To date, the effect of PVA on the synthesis of poly(NIPAm)-based microgels in dispersion polymerizations has been investigated [30,31]. Yates et al. reported the synthesis of PVA-stabilized PNIPAm-based microgels [30]. Guan and Zhang reported that the addition of PVA significantly affected the thermoresponsive properties of phenylboronic acid-functionalized PNIPAm-based microgels [31]. Furthermore, they investigated the effect of PVA molecular weights on the thermoresponsiveness of the PNIPAmbased microgels.
Nanometer-sized polymer gels (nanogels) with a particle size <100 nm are widely used in drug delivery systems for cancer treatments because of their passive targeting properties derived from enhanced permeability and retention effects [32]. However, PNIPAm-based gels generally attain (sub)micrometer-sized particles in precipitation polymerizations. Nanogels have been successfully synthesized via dispersion polymerization using sodium dodecyl sulfate (SDS) as a surfactant [33]. However, SDS is harmful for biological applications. Recently, we have successfully demonstrated the synthesis of PNIPAm-based nanogels via precipitation polymerization (without surfactants) under suitable conditions [34][35][36][37]. Furthermore, the polymer nanogels can recognize intrinsic dysopsonic proteins (serum albumin) and be employed as novel nanocarriers in drug delivery systems [34,38,39]. These polymer nanogels gain stealth capability in situ in the blood vessel by cloaking themselves with intrinsic serum albumin, resulting in their prolonged circulation in the blood [34]. Furthermore, gold nanoparticle-incorporated polymer nanogels prepared via precipitation polymerization have been successfully used as radiation sensitizers [40]. Therefore, the polymer nanogels prepared by precipitation polymerization have been successfully used as drug delivery carriers.
To the best of our knowledge, no studies examining the effect of PVA addition on polymer nanogel formation have been reported. Herein, we investigated the effect of PVA concentration and chain length on the particle formation and thermoresponsive properties of PNIPAm-based nanogels prepared via aqueous dispersion polymerization (Scheme 1). In a series of experiments, we discovered that PVA plays three roles: (i) it bridges the generated polymer chains during polymerization, (ii) it stabilizes the formed polymer nanogels, and (iii) it regulates the thermoresponsive properties of the polymer nanogels when PVA possessing a low molecular weight (degree of polymerization: 500) is used. We believe that the knowledge gained in this study will aid in the future fabrication of functional polymer nanogels.

Effect of PVA Addition on Nanogel Formation
Precipitation polymerization was performed without PVA in 10 mM phosphate buffer (pH 7.4) using N-isopropyl acrylamide (NIPAm), t-butyl acrylamide (TBAm), 2-methacryloyloxyethyl phosphorylcholine (MPC), and N,N -methylenebisacrylamide (MBAA) as monomers. 2,2 -Azobis(2-methylpropionamidine) dihydrochloride (V-50) was selected as the water-soluble initiator. All monomer species and the initiator were dissolved in the solvent prior to polymerization. NIPAm-based polymers are thermoresponsive and exhibit LCST-type phase transitions, owing to the polymer dehydration at high temperatures; thus, the polymers precipitated and assembled as particles to decrease the interfacial free energy during polymerization. The polymers were crosslinked by copolymerizing with MBAA as a crosslinking agent. The conversion of this precipitation polymerization, evaluated using 1 H-nuclear magnetic resonance (NMR), was approximately 100%, with the protons of the vinyl groups of the monomers almost disappearing ( Figure S1). Using dynamic light scattering (DLS), the average particle size of the obtained polymer nanogels (PG 0 ) was determined to be approximately 20 nm (Figure 1), which corresponds with that obtained in our previous study [34].

Effect of PVA Addition on Nanogel Formation
Precipitation polymerization was performed without PVA in 10 mM phosphate buffer (pH 7.4) using N-isopropyl acrylamide (NIPAm), t-butyl acrylamide (TBAm), 2methacryloyloxyethyl phosphorylcholine (MPC), and N,N′-methylenebisacrylamide (MBAA) as monomers. 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (V-50) was selected as the water-soluble initiator. All monomer species and the initiator were dissolved in the solvent prior to polymerization. NIPAm-based polymers are thermoresponsive and exhibit LCST-type phase transitions, owing to the polymer dehydration at high temperatures; thus, the polymers precipitated and assembled as particles to decrease the interfacial free energy during polymerization. The polymers were crosslinked by copolymerizing with MBAA as a crosslinking agent. The conversion of this precipitation polymerization, evaluated using 1 H-nuclear magnetic resonance (NMR), was approximately 100%, with the protons of the vinyl groups of the monomers almost disappearing ( Figure S1). Using dynamic light scattering (DLS), the average particle size of the obtained polymer nanogels (PG0) was determined to be approximately 20 nm (Figure 1), which corresponds with that obtained in our previous study [34]. Transmittance (a) and average particle size (b) of PG0 and PG1000 dispersions at various temperatures. Particle size distributions of obtained PG0 particles (prepared without PVA1000, (c)) and PG1000 particles (prepared with PVA1000, (d)) at 30 °C. PG0 and PG1000 particles were prepared via precipitation and dispersion polymerizations with PVA1000 (3 mg/mL) at 70 °C for 3 h.
The thermoresponsiveness of PG0 was investigated using transmittance measurements. When the temperature reached above the clouding-point temperature of NIPAmbased polymers, their dehydration occurred, resulting in the precipitation of the polymer chains from the aqueous phase. The transmittance of the PG0 dispersions steeply decreased above 40 °C, whereas their clouding-point temperature (the temperature at which polymers show 50% transmittance (T)) was estimated to be approximately 43 °C ( Figure  1). However, the particle size measured using DLS considerably increased to >1000 nm above the clouding-point temperature, indicating that the polymer nanogels coagulated ( Figure 1). The nearly neutral zeta potential of the polymer nanogels (approximately 2.3 mV at 25 °C) indicated that PG0 was stabilized via steric repulsion exhibited by hydrated polymer chains grafted onto the polymer nanogels below the clouding-point temperature, rather than electrostatic repulsion between the cationic chain ends of V-50. However, the polymer chains grafted onto the nanogels do not exhibit steric repulsion above the clouding-point temperature due to dehydration, causing particle coagulation. Transmittance (a) and average particle size (b) of PG 0 and PG 1000 dispersions at various temperatures. Particle size distributions of obtained PG 0 particles (prepared without PVA 1000 , (c)) and PG 1000 particles (prepared with PVA 1000 , (d)) at 30 • C. PG 0 and PG 1000 particles were prepared via precipitation and dispersion polymerizations with PVA 1000 (3 mg/mL) at 70 • C for 3 h.
The thermoresponsiveness of PG 0 was investigated using transmittance measurements. When the temperature reached above the clouding-point temperature of NIPAm-based polymers, their dehydration occurred, resulting in the precipitation of the polymer chains from the aqueous phase. The transmittance of the PG 0 dispersions steeply decreased above 40 • C, whereas their clouding-point temperature (the temperature at which polymers show 50% transmittance (T)) was estimated to be approximately 43 • C ( Figure 1). However, the particle size measured using DLS considerably increased to >1000 nm above the cloudingpoint temperature, indicating that the polymer nanogels coagulated ( Figure 1). The nearly neutral zeta potential of the polymer nanogels (approximately 2.3 mV at 25 • C) indicated that PG 0 was stabilized via steric repulsion exhibited by hydrated polymer chains grafted onto the polymer nanogels below the clouding-point temperature, rather than electrostatic repulsion between the cationic chain ends of V-50. However, the polymer chains grafted onto the nanogels do not exhibit steric repulsion above the clouding-point temperature due to dehydration, causing particle coagulation.
To investigate the effect of PVA on the particle formation, PVA 1000 (polymerization degree: 1000; average saponification degree: 88%, 3 mg/mL) was added to the polymer-ization system. PVA is widely used in biomedical applications as a nanocarrier material or bioadhesive because of its low toxicity [41,42]. Therefore, the PNIPAm-based nanogels were prepared in the presence of PVA 1000 (PG 1000 ). The conversion reached approximately 100% even in the presence of PVA, as evaluated using 1 H-NMR spectroscopy ( Figure S2). This is because the radical concentration in the polymerization system does not significantly change when the radical chain transfer to PVA occurs. The clouding-point temperature of the obtained PG 1000 (approximately 40 • C) estimated from the transmittance measurements was similar to that of the PG 0 (approximately 43 • C) (Figure 1), indicating that the additional amount of PVA 1000 had no significant effect on the phase transition temperature of the gel particles. The transmittance of the gel particle dispersion was reversibly changed by heating and cooling ( Figure S3). However, the particle sizes of PG 0 and PG 1000 significantly differ. Below the clouding-point temperature, PG 1000 had a larger average particle size (approximately 125 nm) than PG 0 (approximately 20 nm). Above the cloudingpoint temperature, the average particle size of PG 1000 remained constant at approximately 350 nm (Figure 1). The particle size distribution of PG 1000 below the clouding-point temperature indicates the bimodal distribution of nanometer-sized and submicrometer-sized particles. However, the particle size distribution was unimodal above the clouding-point temperature (at 60 • C), and the nanometer-sized particles disappeared from the distribution ( Figure 2). These results indicate that the nanometer-sized gel particles coagulated with the submicrometer-sized gel particles above the clouding-point temperature. To investigate whether particle coagulation occurred above clouding-point temperatures, particle size measurements were performed after adding a cationic surfactant [cetyltrimethylammonium bromide (CTAB)] because cationic surfactants suppress the coagulation of destabilized particles above the clouding-point temperatures. Notably, above the clouding-point temperature, the particle size of PG 1000 did not significantly increase in the presence of a cationic surfactant ( Figure S4). These results indicate that the increase in the particle size of PG 1000 above the clouding-point temperatures was caused by the coagulation of the destabilized polymer nanogels. To investigate the effect of PVA on the particle formation, PVA1000 (polymerization degree: 1000; average saponification degree: 88%, 3 mg/mL) was added to the polymerization system. PVA is widely used in biomedical applications as a nanocarrier material or bioadhesive because of its low toxicity [41,42]. Therefore, the PNIPAm-based nanogels were prepared in the presence of PVA1000 (PG1000). The conversion reached approximately 100% even in the presence of PVA, as evaluated using 1 H-NMR spectroscopy ( Figure S2). This is because the radical concentration in the polymerization system does not significantly change when the radical chain transfer to PVA occurs. The clouding-point temperature of the obtained PG1000 (approximately 40 °C) estimated from the transmittance measurements was similar to that of the PG0 (approximately 43 °C) (Figure 1), indicating that the additional amount of PVA1000 had no significant effect on the phase transition temperature of the gel particles. The transmittance of the gel particle dispersion was reversibly changed by heating and cooling ( Figure S3). However, the particle sizes of PG0 and PG1000 significantly differ. Below the clouding-point temperature, PG1000 had a larger average particle size (approximately 125 nm) than PG0 (approximately 20 nm). Above the clouding-point temperature, the average particle size of PG1000 remained constant at approximately 350 nm (Figure 1). The particle size distribution of PG1000 below the clouding-point temperature indicates the bimodal distribution of nanometer-sized and submicrometersized particles. However, the particle size distribution was unimodal above the cloudingpoint temperature (at 60 °C), and the nanometer-sized particles disappeared from the distribution ( Figure 2). These results indicate that the nanometer-sized gel particles coagulated with the submicrometer-sized gel particles above the clouding-point temperature.
To investigate whether particle coagulation occurred above clouding-point temperatures, particle size measurements were performed after adding a cationic surfactant [cetyltrimethylammonium bromide (CTAB)] because cationic surfactants suppress the coagulation of destabilized particles above the clouding-point temperatures. Notably, above the clouding-point temperature, the particle size of PG1000 did not significantly increase in the presence of a cationic surfactant ( Figure S4). These results indicate that the increase in the particle size of PG1000 above the clouding-point temperatures was caused by the coagulation of the destabilized polymer nanogels. Based on these results, PVA appears to play two roles in the particle formation/stabilization: as a stabilizer (primary role) and as a bridging ligand (secondary role). During Based on these results, PVA appears to play two roles in the particle formation/stabilization: as a stabilizer (primary role) and as a bridging ligand (secondary role). During polymerization, mid-chain radicals are generated on PVA via a chain transfer reaction, which initiates the monomer addition reaction. The radical species then undergo a termination reaction with another radical species, yielding dead polymers. During dispersion polymerization, the propagating/dead polymer chains precipitate from the aqueous phase. This series of reactions (chain transfer, propagation, and termination) graft PVA chains on the polymer nanogels, leading to particle stabilization via steric hindrance (primary role of PVA). However, the series of reactions can occur multiple times on the same PVA, inducing bridging between two or more polymer nanogels with a single PVA chain (Scheme 2). The bridging phenomenon (secondary role of PVA) may lead to the formation of submicrometer-sized polymer nanogels.
Molecules 2023, 27, x FOR PEER REVIEW 5 of 11 polymerization, mid-chain radicals are generated on PVA via a chain transfer reaction, which initiates the monomer addition reaction. The radical species then undergo a termination reaction with another radical species, yielding dead polymers. During dispersion polymerization, the propagating/dead polymer chains precipitate from the aqueous phase. This series of reactions (chain transfer, propagation, and termination) graft PVA chains on the polymer nanogels, leading to particle stabilization via steric hindrance (primary role of PVA). However, the series of reactions can occur multiple times on the same PVA, inducing bridging between two or more polymer nanogels with a single PVA chain (Scheme 2). The bridging phenomenon (secondary role of PVA) may lead to the formation of submicrometer-sized polymer nanogels.

Scheme 2.
Schematic of the bridging effect of PVA between different polymer chains in nanogels.

Effect of PVA1000 Concentration
Based on the above findings, we can speculate that the submicrometer-sized gel particles were formed via the PVA1000-induced bridging of multiple nanogels, whereas the nanometer-sized gel particles might have formed without the bridging effect. To verify this hypothesis, we investigated the effect of the PVA1000 concentration on particle formation and stabilization. When the PVA1000 concentration was increased from 2 to 10 mg/mL (at 35 °C, below the clouding-point temperature), the average size of the gel particles gradually increased (Figure 3), and the peak corresponding to the nanometer-sized gel particles disappeared when 10 mg/mL of PVA1000 was used (Figure 2). These results indicate that the submicrometer-sized gel particles were formed because of the bridging effect of PVA1000. Notably, the clouding-point temperatures determined using the transmittance measurements were similar for all PVA1000 concentrations, indicating that the PVA1000 concentration did not affect the phase transition temperature of PG1000 during polymerization ( Figure S5). Furthermore, the particle size distributions obtained all PVA1000 concentrations were unimodal above the clouding-point temperature (at 60 °C), indicating that the submicrometer-sized polymer gel particles were colloidally stable even Scheme 2. Schematic of the bridging effect of PVA between different polymer chains in nanogels.

Effect of PVA 1000 Concentration
Based on the above findings, we can speculate that the submicrometer-sized gel particles were formed via the PVA 1000 -induced bridging of multiple nanogels, whereas the nanometer-sized gel particles might have formed without the bridging effect. To verify this hypothesis, we investigated the effect of the PVA 1000 concentration on particle formation and stabilization. When the PVA 1000 concentration was increased from 2 to 10 mg/mL (at 35 • C, below the clouding-point temperature), the average size of the gel particles gradually increased (Figure 3), and the peak corresponding to the nanometersized gel particles disappeared when 10 mg/mL of PVA 1000 was used (Figure 2). These results indicate that the submicrometer-sized gel particles were formed because of the bridging effect of PVA 1000 . Notably, the clouding-point temperatures determined using the transmittance measurements were similar for all PVA 1000 concentrations, indicating that the PVA 1000 concentration did not affect the phase transition temperature of PG 1000 during polymerization ( Figure S5). Furthermore, the particle size distributions obtained all PVA 1000 concentrations were unimodal above the clouding-point temperature (at 60 • C), indicating that the submicrometer-sized polymer gel particles were colloidally stable even above the clouding-point temperature (Figure 2). These results clearly indicate that the addition of PVA 1000 led to both the stabilization and bridging of polymer gel particles and/or polymer chains during polymerization (Scheme 3). Thus, the polymer gel particles Molecules 2023, 28, 3493 6 of 12 may have crosslinked by MBAA and PVA. Furthermore, the grafting density of the polymer gel particles may increase with increasing PVA concentration because PVA can act as a bridging agent (crosslinker) between different nanogel particles and/or polymer chains. However, a detailed investigation is necessary to evaluate the crosslinking density of the polymer gel particles using light or X-ray scattering.
Molecules 2023, 27, x FOR PEER REVIEW 6 of 11 above the clouding-point temperature (Figure 2). These results clearly indicate that the addition of PVA1000 led to both the stabilization and bridging of polymer gel particles and/or polymer chains during polymerization (Scheme 3). Thus, the polymer gel particles may have crosslinked by MBAA and PVA. Furthermore, the grafting density of the polymer gel particles may increase with increasing PVA concentration because PVA can act as a bridging agent (crosslinker) between different nanogel particles and/or polymer chains. However, a detailed investigation is necessary to evaluate the crosslinking density of the polymer gel particles using light or X-ray scattering.  above the clouding-point temperature (Figure 2). These results clearly indicate that the addition of PVA1000 led to both the stabilization and bridging of polymer gel particles and/or polymer chains during polymerization (Scheme 3). Thus, the polymer gel particles may have crosslinked by MBAA and PVA. Furthermore, the grafting density of the polymer gel particles may increase with increasing PVA concentration because PVA can act as a bridging agent (crosslinker) between different nanogel particles and/or polymer chains. However, a detailed investigation is necessary to evaluate the crosslinking density of the polymer gel particles using light or X-ray scattering.

Effect of PVA Chain Length
To obtain stable polymer nanogels, we regulated the bridging and stabilization effects of PVA on the obtained polymer nanogels by changing the PVA chain length. We investigated the effect of the PVA chain length on the particle size and colloidal stability using three different PVAs with varying degrees of polymerization (500: PVA 500 , 1000: PVA 1000 , and 3500: PVA 3500 ). When PVA with a longer chain length was used, the PVA-induced bridging effect of different polymer chains/nanogels was increased, and the large gel particles with high colloidal stability were obtained. However, the bridging effect decreased when PVA with a shorter chain length was used, and small gel particles were obtained owing to the suppression of the bridging effect of PVA. Figure 4 shows the transmittance of the obtained polymer gel particle dispersions prepared using three different types of PVA. When the polymerization degree of PVA was increased to 3500 (PVA 3500 ), the clouding-point temperature of the obtained polymer gel particles (PG 3500 ) was not significantly shifted to a lower temperature compared to that of PG 1000 . Furthermore, the transmittance below the clouding-point temperature was slightly low (~75%), implying the formation of larger polymer gel particles. In fact, submicrometer-sized gel particles were obtained using PVA 3500 (PG 3500 ) below the cloudingpoint temperature. These results support our hypothesis that PVA assists the bridging of different polymer chains/nanogels during dispersion polymerization. Importantly, the thermoresponsiveness of PG 3500 was different from that of PG 1000 . The particle size of PG 1000 significantly increased above the LCST, whereas that of PG 3500 decreased ( Figure 4). This phenomenon exhibited by PG 3500 is observed for submicrometer-sized gel particles with LCST-type thermoresponsive properties [43]. Furthermore, PG 3500 particles showed a similar decreasing trend even in the presence of CTAB. This implies that PG 3500 did not coagulate, but rather shrank because of dehydration above the clouding-point temperatures.

Effect of PVA Chain Length
To obtain stable polymer nanogels, we regulated the bridging and stabilization effects of PVA on the obtained polymer nanogels by changing the PVA chain length. We investigated the effect of the PVA chain length on the particle size and colloidal stability using three different PVAs with varying degrees of polymerization (500: PVA500, 1000: PVA1000, and 3500: PVA3500). When PVA with a longer chain length was used, the PVAinduced bridging effect of different polymer chains/nanogels was increased, and the large gel particles with high colloidal stability were obtained. However, the bridging effect decreased when PVA with a shorter chain length was used, and small gel particles were obtained owing to the suppression of the bridging effect of PVA. Figure 4 shows the transmittance of the obtained polymer gel particle dispersions prepared using three different types of PVA. When the polymerization degree of PVA was increased to 3500 (PVA3500), the clouding-point temperature of the obtained polymer gel particles (PG3500) was not significantly shifted to a lower temperature compared to that of PG1000. Furthermore, the transmittance below the clouding-point temperature was slightly low (~75%), implying the formation of larger polymer gel particles. In fact, submicrometer-sized gel particles were obtained using PVA3500 (PG3500) below the clouding-point temperature. These results support our hypothesis that PVA assists the bridging of different polymer chains/nanogels during dispersion polymerization. Importantly, the thermoresponsiveness of PG3500 was different from that of PG1000. The particle size of PG1000 significantly increased above the LCST, whereas that of PG3500 decreased ( Figure 4). This phenomenon exhibited by PG3500 is observed for submicrometer-sized gel particles with LCST-type thermoresponsive properties [43]. Furthermore, PG3500 particles showed a similar decreasing trend even in the presence of CTAB. This implies that PG3500 did not coagulate, but rather shrank because of dehydration above the clouding-point temperatures. Figure 4. Transmittance of PG500, PG1000, and PG3500 particle dispersions prepared via dispersion polymerization (a). Average particle sizes (circles) and PDI (triangles) of PG500 (b,e), PG1000 (c,f), and PG3500 (d,g) particles at various temperatures without (b-d) or with (e-g) CTAB. Particle size distributions of PG500 (h,i) and PG3500 (j,k) particles at 60 °C without (h,j) and with (i,k) CTAB. Polymer Figure 4. Transmittance of PG 500 , PG 1000 , and PG 3500 particle dispersions prepared via dispersion polymerization (a). Average particle sizes (circles) and PDI (triangles) of PG 500 (b,e), PG 1000 (c,f), and PG 3500 (d,g) particles at various temperatures without (b-d) or with (e-g) CTAB. Particle size distributions of PG 500 (h,i) and PG 3500 (j,k) particles at 60 • C without (h,j) and with (i,k) CTAB. Polymer gel particles were prepared via dispersion polymerization with PVA polymer chains of different lengths (polymerization degrees: 500, 1000, and 3500) at 70 • C for 3 h.
The thermoresponsive properties and colloidal stability of the polymer gel particles prepared using PVA 500 (PG 500 ) were different from those of PG 1000 and PG 3500 . The particle size of PG 500 was less than 100 nm at temperatures lower than the clouding-point temperature ( Figure 4). Furthermore, the nanometer-sized polymer nanogels were clearly visible in transmittance electron microscopy images when the PNIPAm-based nanogels were prepared with PVA 500 (Figure 5). The nanometer size of PG 500 particles was maintained up to 60 • C, while the particles coagulated above 65 • C (Figure 4). The clouding-point temperature estimated from the transmittance measurements of PG 500 was approximately 63 • C, which was higher than those of PG 1000 and PG 3500 . This may be attributed to the insertion of PVA 500 into the polymer main chains. Previous studies have revealed that the copolymerization of a hydrophilic monomer with PNIPAm shifts the LCST to a higher temperature [44]. Furthermore, a negligible increase in the particle size of PG 500 was observed above 60 • C when CTAB was added during DLS measurements. These results indicate that the increase in particle size of PG 500 (without CTAB) above 60 • C was caused by coagulation. The colloidal stability of PG 500 at high temperatures (up to 60 • C) was induced by the steric repulsion of grafted PVA 500 . These results suggest that the bridging effect between the different polymer nanogels weakened with decreasing polymerization degree of PVA, whereas PVA-induced colloidal stability was afforded to the polymer nanogels. Therefore, we can conclude that the different degrees of polymerization of PVA affected the formation of polymer gel particles during dispersion polymerization by controlling the bridging effect between different polymer chains/nanogels. Furthermore, to investigate whether PVA was covalently bonded in PNIPAm nanogels, we prepared the PNIPAm nanogels with fluorescein-labeled PVA. The fluorescence spectrum of the fluorescein-labeled PVAcontaining polymer gels as a dispersed state was obtained ( Figure S6). The spectra clearly indicate that the PVA was covalently bonded in PNIPAm nanogels. pared with PVA500 ( Figure 5). The nanometer size of PG500 pa 60 °C, while the particles coagulated above 65 °C (Figure 4). ture estimated from the transmittance measurements of PG which was higher than those of PG1000 and PG3500. This may of PVA500 into the polymer main chains. Previous studies ha merization of a hydrophilic monomer with PNIPAm shifts th ture [44]. Furthermore, a negligible increase in the particle above 60 °C when CTAB was added during DLS measurem that the increase in particle size of PG500 (without CTAB) abov ulation. The colloidal stability of PG500 at high temperatures ( the steric repulsion of grafted PVA500. These results suggest tween the different polymer nanogels weakened with decre of PVA, whereas PVA-induced colloidal stability was afford Therefore, we can conclude that the different degrees of pol the formation of polymer gel particles during dispersion po the bridging effect between different polymer chains/nanog gate whether PVA was covalently bonded in PNIPAm nanoge nanogels with fluorescein-labeled PVA. The fluorescence sp beled PVA-containing polymer gels as a dispersed state wa spectra clearly indicate that the PVA was covalently bonded

Conclusions
In this study, we investigated the effect of PVA addition mation during dispersion polymerization. Our results indica fects on the polymer nanogels: (i) bridging between different ing polymerization, (ii) stabilization of the polymer nanogels moresponsiveness. In the presence of PVA with a highe (PVA3500), microgel particles were formed during dispersion

Conclusions
In this study, we investigated the effect of PVA addition on the polymer nanogel formation during dispersion polymerization. Our results indicate that PVA induces three effects on the polymer nanogels: (i) bridging between different polymer chains formed during polymerization, (ii) stabilization of the polymer nanogels, and (iii) regulation of thermoresponsiveness. In the presence of PVA with a higher degree of polymerization (PVA 3500 ), microgel particles were formed during dispersion polymerizations, owing to the enhanced bridging effect. On the contrary, the nanogels that are stable over a wide temperature range (under 60 • C) were obtained using PVA 500 . The nanotechnology devel-oped herein may help in further advancing research on polymer nanogels in fields such as biomedicine, wherein the fabrication of nanocarriers for targeted drug delivery and other similar functions may be possible.

Apparatus
UV-visible (UV-Vis) spectral measurements were conducted using a V-560 spectrophotometer (Jasco Ltd., Tokyo, Japan). 1 H NMR spectra were measured using a 400-MHz Fourier transform (FT)-NMR apparatus (JNM-ECX400 FT-NMR system, JEOL Ltd., Tokyo, Japan). The particle size distribution and the zeta potential of the obtained particles were measured using ZETASIZER NANO-ZS (Malvern, UK).

Transmittance Measurements
As-prepared particle dispersions (3 mL) were poured into the UV-Vis cell, and the transmittance measurement (λ = 600 nm) was conducted. The temperature was increased at a constant rate (0.5 • C/min). The reversibility of the thermoresponsiveness of the obtained particles was evaluated by alternately increasing and decreasing the temperature at a constant rate (0.5 • C/min).

Particle Size Distributions
The particle size distributions of the as-prepared particle dispersions (3 mL) were measured using DLS at different temperatures (25,30,35,40,45, 50, 60, and 70 • C). The measurements were performed sequentially at 600 s intervals. To investigate the effect of surfactants on particle aggregation, CTAB (~1 mg/mL) was added to the particle dispersion.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules28083493/s1. Figure S1: The 1 H-NMR spectra of precipitation polymerization, Figure S2: The 1 H-NMR spectra of dispersion polymerization with PVA, Figure S3: reversibility of the transmittance changes of polymer nanogel dispersion, Figure S4: particle size change of nanogels in the presence of cationic surfactants, Figure S5: and cloudingpoint temperatures of PG 1000 prepared with different PVA 1000 concentrations, Figure S6: Dispersion polymerization with fluorescein-labeled PVA 500 .