Artemisia vestita: A Folk Medicine with Hidden Herbal Fortune

Traditional medicines are nature’s gift and our native heritage, which play a vital role in maintaining a disease-free life. Artemisia vestita Wall. ex Besser (family: Asteraceae), popularly known as “Kubsha” or “Russian wormwood”, is a highly enriched folklore medicine with wound- healing, antiphlogistic, antifebrile, antifeedant, anti-helminthic, antimicrobial, antiviral, antitumor, and antiproliferative potential attributed to the presence of various volatile and non-volatile secondary metabolites. A systematic and extensive review of the literature on A. vestita was carried out via the Web of Science, PubMed, INMEDPLAN, EMBASE, Google Scholar, and NCBI, as well as from several websites. The highly relevant literature contained in 109 references was selected for further inclusion in this review. A total of 202 bioactive compounds belonging to different chemical classes such as terpenoids, coumarins, flavonoids, alkaloids, acetylenes, tannins, carotenoids, and sterols have been reported in A. vestita, which are responsible for different pharmacological activities. The chemical structures obtained from the PubChem and Chem Spider databases were redrawn using the software Chem Draw® version 8.0. This review paper summarizes the distribution, botanical description, phytochemistry, pharmacological activities, and conservation of A. vestita, which will assist scientists for further investigation. Extensive studies on the active constituents, pharmaceutical standardization, mode of action, and sustainable conservation of A. vestita are needed to further explore its wound-healing and allied medicinal properties.


Introduction
The world is enriched with a treasure trove of traditional medicinal herbs that are of global importance for health security. India harbours four mega-biodiversity hotspots and is highly enriched with 17,500 species of medicinal plants used effectively against multiple disorders [1][2][3]. These medicinal herbs have been time-tested and recommended by saints, maharishis, vaidyas, and ayurvedic acharyas and have strong credence in different traditional medicinal systems such as ayurveda, unani, siddha, homeopathy, naturopathy, allopathy, and traditional Chinese medicine for treating ailments related to both humans and animals. Moreover, these medicines are safe, heal the cause of the ailment, and have less or no side effects compared to allopathic drugs [4]. For centuries, plant essential oils have played a provocative role for mankind. The people of Egypt were known to be skilled perfumers and taught the art of perfumery to Hebrews around 5000 years ago [5].
Earlier naturalized or wild plants provided social security to people in the form of supplements, fuel, fodder, raw material for companies, and an additional good income source. According to the WHO, approximately 80% of people are reliant on herbal remedies in developing nations. A total of 90% of herbal species used in India are brought from the western Himalayas, which is well known for its rich plant diversity, with 1748 medicinal species used in various fields such as pharmacological research, chemistry, clinical therapeutic studies, and pharmacognosy. Unfortunately, the traditional knowledge of To date, there have been fragmentary reports on A. vestita extracts, their mode of action, standardization, dose optimization, and toxicity. Through this article, we have tried to bridge the gap and provide explicit information on the distribution, botanical description, phytochemistry, and pharmacological activities of Russian wormwood.

Distribution
Artemisia vestita is widely distributed in East Asia including the Himalayas of Nepal, Pakistan, India, and Tibet to southern and central China (Figure 1), including hills, rocky slopes, grasslands, shrublands, and outer forest margins of various regions of Gansu, northwest Guangxi, north Hubei, Guizhou, Liaoning, west Sichuan, Qinghai, Xizang, Xinjiang, Yunnan, north India, Nepal, and north Pakistan. Due to geographical variation and seasonal factors, drastic variations have been reported in the chemical composition of the essential oil. In India, Drubsha is found in Himachal Pradesh, Kashmir, and Uttarakhand at an altitude of 2100-3000 m. The distribution of A. vestita in the Pooh region of Kinnaur district at different elevations noted a density of 1.27/ha, frequency of 10%, abundance of 12.67, ratio of abundance to frequency (A/F) of 1.27, and importance value index of 5.12 at an elevation of 2700-3200 m; a density of 2.25/ha, frequency of 15%, abundance of 15, A/F of 1, and IVI of 7.28 at an elevation of 3200-3700 m; and a density of 1.17/ha, frequency of 11.67%, abundance of 10, A/F of 0.86, and IVI of 53.53 at an elevation of 3700-4200 m [32]. Important value indexes are the sum of the frequency, density, and dominance of the individual species. Tyethven' [31]. It is also commonly known as 'Russian Wormwood', 'Ganga Tulsi', 'Buer', 'Drubsha', 'Seski, Kubsha', 'Chamariya', 'Kundja', and 'Kundiyaa'. To date, there have been fragmentary reports on A. vestita extracts, their mode of action, standardization, dose optimization, and toxicity. Through this article, we have tried to bridge the gap and provide explicit information on the distribution, botanical description, phytochemistry, and pharmacological activities of Russian wormwood.

Distribution
Artemisia vestita is widely distributed in East Asia including the Himalayas of Nepal, Pakistan, India, and Tibet to southern and central China (Figure 1), including hills, rocky slopes, grasslands, shrublands, and outer forest margins of various regions of Gansu, northwest Guangxi, north Hubei, Guizhou, Liaoning, west Sichuan, Qinghai, Xizang, Xinjiang, Yunnan, north India, Nepal, and north Pakistan. Due to geographical variation and seasonal factors, drastic variations have been reported in the chemical composition of the essential oil. In India, Drubsha is found in Himachal Pradesh, Kashmir, and Uttarakhand at an altitude of 2100-3000 m. The distribution of A. vestita in the Pooh region of Kinnaur district at different elevations noted a density of 1.27/ha, frequency of 10%, abundance of 12.67, ratio of abundance to frequency (A/F) of 1.27, and importance value index of 5.12 at an elevation of 2700-3200 m; a density of 2.25/ha, frequency of 15%, abundance of 15, A/F of 1, and IVI of 7.28 at an elevation of 3200-3700 m; and a density of 1.17/ha, frequency of 11.67%, abundance of 10, A/F of 0.86, and IVI of 53.53 at an elevation of 3700-4200 m [32]. Important value indexes are the sum of the frequency, density, and dominance of the individual species.

Morphology
A. vestita Wall. ex Besser is an aromatic, erect, perennial shrub that may attain a height of 2 m (5-120 cm) [33]. The leaves are fern-like, soft, and hairy on the upper surface white and hairy towards the lower side, and pinnately cut. The flowers are small and appear creamy yellow, arranged in racemes (6)(7)(8)(9)(10); the heads of flowers are long, hairy, and compound, hanging gracefully on their slender nodding stalks. The fruits are shiny and smooth and the bracts are oblong and membranous ( Figure 2). The leaves have clear abaxial and adaxial surfaces. Adaxial surface-elongated epidermal cells are partitioned and surface grooved with prominently ridged margins; the tertiary sculpture is aggregated; stomata are depressed,

Morphology
A. vestita Wall. ex Besser is an aromatic, erect, perennial shrub that may attain a height of 2 m (5-120 cm) [33]. The leaves are fern-like, soft, and hairy on the upper surface white and hairy towards the lower side, and pinnately cut. The flowers are small and appear creamy yellow, arranged in racemes (6-10); the heads of flowers are long, hairy, and compound, hanging gracefully on their slender nodding stalks. The fruits are shiny and smooth and the bracts are oblong and membranous ( Figure 2). The leaves have clear abaxial and adaxial surfaces. Adaxial surface-elongated epidermal cells are partitioned and surface grooved with prominently ridged margins; the tertiary sculpture is aggregated; stomata are depressed, surrounded by thick, flat peristomal rims, transverse striata, thick inner ledges, and a gradually concave surface of the guard cells and transverse polar folds. The lobule tips are cap-like and swollen; stomata are also present in this particular region, with cells greatly undulated (V-shaped undulations) and elongated. The abaxial surface-cell outline is similar, somehow deeply undulate, with loose 'V'-shaped undulations; it is tertiary sculptured and coarsely granular; the guard cells have oblique folds [34][35][36].
Molecules 2023, 28, x FOR PEER REVIEW 4 of 23 surrounded by thick, flat peristomal rims, transverse striata, thick inner ledges, and a gradually concave surface of the guard cells and transverse polar folds. The lobule tips are cap-like and swollen; stomata are also present in this particular region, with cells greatly undulated (V-shaped undulations) and elongated. The abaxial surface-cell outline is similar, somehow deeply undulate, with loose 'V'-shaped undulations; it is tertiary sculptured and coarsely granular; the guard cells have oblique folds [34][35][36].

Vegetation Details
A. vestita can dominate the grassland ecosystem [37]. The A. vestita plants are hermaphrodite and pollinated by insects, and the seeds ripen in the months of August-October.
Habitat: Woodland edge garden, sunny position, cultivated beds, hills, rocky slopes, grasslands, shrublands, and exterior forest margins at an altitude of 2000-4300 m above sea level [38]. Cytology: Gupta et al. [39] reported 2n = 2x = 36 meiotic chromosome count, ploidy level (4×), pollen fertility 78-82%, and pollen grain size 22-24 μm in A. vestita collected from Haripurdhar and Churdhar (HP) at an altitude of 2400 and 3650 m, respectively, above sea level. Two other cytotypes, i.e., hexaploid (2n = 54) [40] and diploid (2n = 18) [41], have also been reported. Chlorophyll content: Variations in the chlorophyll and anthocyanin content and the chlorophyll/carotenoid ratio have been reported in the temperate species of A. vestita found at an altitude of 550 and 3600 m above sea level in the Garhwal Himalayas. The total chlorophyll content observed in the lower leaves of A. vestita was 1.716 and 1.470, middle leaves 0.902 and 1.650, and top leaves 0.863 and 1.205 at two different altitudes (550 and 3600 m, respectively) [42]. The molar chlorophyll/carotenoid ratio was observed to be lower (1) in the temperate species as compared to the tropical (1.7) and subtropical species (1.3) [43]. At higher altitudes, A. vestita plants have relatively broader adaptability potential compared to lowland species, which is due to a higher osmotic concentration, greater lignification, and the tendency of osmoregulation in tissues, due to the conversion of starch into sugar content [44]. A palynological study of A. vestita stated the quantitative characteristics of the plant, such as a polar axis of 19.38 ± 1.52μm, equatorial axis of 18.09 ± 1.51 μm, P/E (sphericity) of 1.07, thickness of exine of 2.13 ± 0.67 μm, and colpus length of 11.81 ± 1.69 μm, and spinules are prominent in the plant [45].

Vegetation Details
A. vestita can dominate the grassland ecosystem [37]. The A. vestita plants are hermaphrodite and pollinated by insects, and the seeds ripen in the months of August-October.
Habitat: Woodland edge garden, sunny position, cultivated beds, hills, rocky slopes, grasslands, shrublands, and exterior forest margins at an altitude of 2000-4300 m above sea level [38]. Cytology: Gupta et al. [39] reported 2n = 2x = 36 meiotic chromosome count, ploidy level (4×), pollen fertility 78-82%, and pollen grain size 22-24 µm in A. vestita collected from Haripurdhar and Churdhar (HP) at an altitude of 2400 and 3650 m, respectively, above sea level. Two other cytotypes, i.e., hexaploid (2n = 54) [40] and diploid (2n = 18) [41], have also been reported. Chlorophyll content: Variations in the chlorophyll and anthocyanin content and the chlorophyll/carotenoid ratio have been reported in the temperate species of A. vestita found at an altitude of 550 and 3600 m above sea level in the Garhwal Himalayas. The total chlorophyll content observed in the lower leaves of A. vestita was 1.716 and 1.470, middle leaves 0.902 and 1.650, and top leaves 0.863 and 1.205 at two different altitudes (550 and 3600 m, respectively) [42]. The molar chlorophyll/carotenoid ratio was observed to be lower (1) in the temperate species as compared to the tropical (1.7) and subtropical species (1.3) [43]. At higher altitudes, A. vestita plants have relatively broader adaptability potential compared to lowland species, which is due to a higher osmotic concentration, greater lignification, and the tendency of osmoregulation in tissues, due to the conversion of starch into sugar content [44]. A palynological study of A. vestita stated the quantitative characteristics of the plant, such as a polar axis of 19.38 ± 1.52µm, equatorial axis of 18.09 ± 1.51 µm, P/E (sphericity) of 1.07, thickness of exine of 2.13 ± 0.67 µm, and colpus length of 11.81 ± 1.69 µm, and spinules are prominent in the plant [45].

Medicinal Uses
A. vestita has been used as a folkoric medicine and was harvested from wild forests for use in anti-inflammatory and antifebrile medicines. Both aqueous and alcoholic solvents give a maximum amount of medicinal extract from the plant compared to other solvents [46]. It is widely used for treating numerous inflammatory diseases in Tibet and China, such as contact dermatitis, rheumatoid arthritis, and sepsis [47,48]. The leaves are crushed and applied externally on the skin as hemostatic [49,50]. The plant is also used in treating stomach-aches [51].

Essential Oil
A total of 202 biochemical compounds have been reported from different parts (stem, leaves, roots) of A. vestita. There are mainly flavonoids, terpenoids, oxygenated monoterpenes, sesquiterpene lactones, oxygenated sesquiterpenes, sesquiterpenoids, hydroxyl cinnamic acids, monoterpene hydrocarbons, azulenes, sesquiterpene hydrocarbons, sterols, phenylpropanoids, monoterpenoids, hydroxycoumarins, coumarins, flavonoid glycosides, organosulfonic acids, oxygenated triterpenes, and aromatic aldehydes. The chemical structures of the bioactive compounds present in Russian wormwood are shown in Figure 3. The characteristic oil odour is determined by the constituents of fresh-smelling thujone and eucalyptol (1,8-cineole) and by the woody bark and the sweet note of atlantone and himachalol compounds. The odour of the plant's essential oil is mainly woody, herbaceous, fresh, slightly sweet, and reminiscent of the sage and balsamic odour and is reported to be effective against dermatophytes [79]. The oil can be used safely in perfumery, scented soaps, and cosmetics. Camphor, borneol, and eucalyptol are responsible for the pleasant aroma and are also known for their antifungal and antibacterial activities. The flowering tops of the plants when subjected to steam distillation yield yellow-, orange-, or brown-coloured oil with an aromatic, sweet-woody odour [70]. The essential oil was produced from A. vestita from the Nainital hills, Shimla hills, and Kashmir valley [80,81]. The physiochemical characteristics observed in the essential oil of A. vestita are a refractive index D of 1.4915, ester value of 55.45, acid value of 1.3, carbonyl percentage of 20.80, ester value of 124.4 after acetylation, 1:1 solubility (in 95% alcohol), and specific gravity of 0.910 [70].

Wound-Healing
Fresh leaf paste is applied on wounds or cuts to stop the bleeding and inflammation [89]. Chinese medicines include A. vestita as an alternative and complementary medicine for the treatment of skin diseases. A. vestita has a cold nature and is capable of treating skin eruptions, heat, and itching [90]. Pastes of the leaves are applied for the treatment of skin infections, inflammation, ringworm, wounds, respiratory tract infections, ethnic therapy for colds, sinus drainage, and asthma [14,[91][92][93][94].

Antidote
A. vestita has been used for the treatment of snake bite due to its high content of monoterpenes, flavones, and sesquiterpenoids in the leaf extracts.

Antimicrobial
The A. vestita essential oils are mainly composed of an odoriferous mixture of sesquiterpenes, monoterpenes, and aromatic compounds, which are used in naturopathy are very well known for their antimicrobial properties. Two major compounds, namely, grandisol and 1,8-cineol, have shown in vivo and in vitro antibacterial activity against respiratoryinfection-causing bacteria. The oil exhibited MIC (minimum inhibitory concentration) values between 20 and 80 µg/mL, whereas the constituents exhibited between 130 and 200 µg/mL. The in vivo studies showed significant results of the oil and its component grandisol, which did not produce any toxic effects in mice [83]. Eight components that have been reported to exhibit antibacterial activity are αand β-thujone, terpinen-4-ol, linalool, nerol, geraniol, α-pinene, and 1,8-cineole; their percentages in the oil were determined in ref. [84]. The plant extract and formulated gel have also shown significant results, as the plant extract exhibited MIC values between 100 and 240 µg/mL while the formulated gel (extract + natural polymer) exhibited MIC values between 30 and 85 µg/mL (unpublished work) against skin-infection-causing bacterial and fungal species.

Immunosuppressive Activity
Plant constituents are useful in healing immunological disorders such as autoimmune disorders and also in organ transplantation, as they produce immune-suppressive agents [95]. It has been reported that the essential oil of plants with higher amounts of αand β-thujone have lesser or trace amounts of eucalyptol and camphor [96]. Jaceosidin exerts immunosuppressive effects both in vitro and in vivo through the activation and inhibition of T-cell proliferation, which is associated with the down-regulation of interferon (IFN)-gamma signal transducers and activators of the (STAT1) transcription1 and transcription factor T-box TBX-21 signaling pathway [72]. In addition, flavones such as apigenin, cirsilineol, and 6-methoxytricin from A. vestita have shown immunosuppressive and anti-inflammatory effects [14]. These flavones specifically inhibit PCA (passive cutaneous anaphylaxis), which induces contact hypersensitivity, whereas lymphocyte proliferation is induced by Con-A and CD-25 expression in T-cells, which shows immunosuppressive effects.

Anti-Inflammatory Activity
A. vestita extracts exhibit anti-inflammatory activity such as degranulation inhibition in mast cells and inflammatory cytokine production [63]. The plant extracts inhibited the proliferation of mouse splenocytes and mixed lymphocytes while reducing the IL-2 interleukin level and the level of metallo-proteinase-9 in vivo and in vitro [14].
The LD 50 of less than 1000 mg/Kg was devoid of antiprotozoal, antibacterial, antifungal, antiviral, anthelmintic, diuretic, hypoglycaemic and anticancer, which showed its effect on isolated guinea pig respiration, ileum, nictitating membrane, cardiovascular system, and central nervous system [50]. A. vestita extract cross-linked with tragacanth gum (crosslinked polyacrylic-acid-based hydrogel) showed anti-inflammatory activity. The inhibition exhibited by a plant extract and plant extract formulated gel with a natural polymer of COX-1 (Cyclooxygenase-1) was found to be 97.962 ± 0.892% and 69.812 ± 0.911%, respectively, at 500 µg/mL concentration. Similarly, our group also explored a significant inhibition of cyclooxygenase-2. The inhibition percentage by the plant extract and plant extract formulated gel were 89.47 ± 1.401% and 52.76 ± 1.110%, respectively (unpublished work).

Anti-Epileptic Activity
Hispidulin is a flavonoid naturally occurring in A. vestita with powerful anti-epileptic activity. Hispidulin showed a 21.1893 Kcal/mol in silico docking score while targeting the human enzyme glycogen phosphorylase-b/chrysin, which showed the greater potential of this inhibitor molecule to become an effective antidiabetic drug to control hyperglycemia in type-2 diabetes. However, the work demands thorough in vivo and in vitro studies for the molecules to be used as anti-hyperglycemic drugs [85,97].

Antifeedant Activity
The compound artemivestinolide showed antifeedant activities against the third-instar larvae of Plutella xylostella with EC50 values of 25.3-42 and against the phytopathogenic fungi F. oxysporum (MIC-256 mg/L), P. oryzae (MIC-128 mg/L), and B. cinerea (MIC-256 mg/L) [86]. A. vestita ethanolic extract exhibited anti-inflammatory, anti-helminthic, and insecticidal activity against Haemonchus contortus and Sitophilus zeamais, respectively [60,65,86]. The whole-plant extract containing vegetative shoot exhibited a 87.2% reduction in the faecal egg count at 100 mg/kg, which showed significant activity against adult worms and larvae after 28 days post-treatment [86]. In a study, essential oil of A. vestita showed potential fumigant activity with an LC 50 value of 13.42 mg/L and LD 50 value of 50.62 mg against adult Sitophilus zeamais in the fumigant bioassay and in the contact bioassay, respectively [65]. Several monoterpenes, sesquiterpenoids, and flavones have been isolated from A. vestita [20,72] and the essential oil chemical composition has been well-studied [69,70]. 1,8-cineol has a cold-relieving effect with mucolytic and expectorant properties [98]. 1,8cineol and camphor present in plant essential oil act as fumigants with a broad insecticidal activity [99][100][101] and possess the potential to expand as a novel natural fumigant for insect control in stored products [102,103]. They are advantageous over conventional fumigants because they are non-persistent, biodegradable, and easily procurable and exhibit low toxicity to mammals [104].

Known Hazards
Although no specific reports on toxicity have been recorded for A. vestita extracts, the genus Artemisia contains allergenic sesquiterpenoid lactones that have the potential to cause skin reactions or dermatitis [105].

Cytotoxicity
The presence of volatile terpenoids and monoterpenes, i.e., pinene, eugenol, 1,8cineole, limonene, citronellol, terpinolene, citronellal, thymol, and camphor, in A. vestita essential oil constituents provides repellent or toxic activity [106]. Compounds named Arvestolides H and I showed inhibitory effects in BV-2 cells on nitric oxide production, which was induced by lipopolysaccharide with an IC 50 value of 43.2 µM for Arvestolides H and 39.9 µM for Arvestolides I. [61], whereas cirsilineol, apigenin, and 6-methoxytricin are the active components that inhibited the proliferation of T-cells and the activation of in vitro bioassays. Immune-suppressive compounds extracted from A. vestita will be an effective remedy for T-cell-mediated inflammation [14]. Sesquiterpenes, coumarins, and flavones were reported in wormwood [107]. The aqueous leaf extract alleviates picryl chloride (PCl)-induced contact hypersensitivity by blocking the T lymphocyte activation [108]. In a study, flow cytometric and MTT assays were used for determining the CD 25 expression in T-cells and the proliferation of Con A induced lymphocytes [14]. For cytotoxic effects, both S2 (extract) and S4 (extract + polymer) showed higher cell viability. At a 1000 µg/mL concentration of S2, the cytotoxicity to HaCat cells was 18.2 ± 0.35% compared to that of S4, which was 19.7 ± 0.29%. The results showed promising anti-inflammatory effects along with significant anti-cancer effects on HaCat cell lines (unpublished work). The aforementioned results provide A. vestita-based folklore medicine a rationale to be used in wound-healing and anti-cancer therapy.

Biological Activity of Annphenone
Annphenone showed specific and potent antiproliferative activity against HepG2 cells and the IC50 value was 2.0 ± 0.4 µg/mL. During the cell cycle analysis in the G0/G1 phase, annphenone compound arrested the HepG2 cells when detecting the immunocytochemistry. It is suggested that the annphenone compound inhibits the catenin expression induced by the localization transfer, reducing the cyclin D1 protein expression. Furthermore, annphenone's interaction as a possible ligand of the ASGP-R asialoglycoprotein receptor using a molecular docking simulation revealed its selectivity for hepatocellular carcinoma cells and potentially specific for antiproliferative activity [71]. Annphenone is a promising anti-tumour agent present in the aqueous extract that reduced the contact sensitivity via down-regulating the adhesion, activation, and production of metalloproteinase Tlymphocytes in mice [109], whereas the ethanolic extract exerted anti-sepsis activity through down-regulation of the NF-kB and MAPK pathways [63].

Biological Activity of Cirsilineol
Cirsilineol (4 ,5-dihydroxy-3 ,6,7-trimethoxyflavone) found in A. vestita extracts possesses potent anti-tumour and immune suppressive properties [14,87]. Cirsilineol significantly inhibited the proliferation of multiple types of cancer cells (Skov-3, PC3, Caov-3, and Hela cells) in a concentration-dependent manner. It induced apoptosis in Caov-3 cells in a dose-dependent manner, which was determined with annexin V/propidium iodide double staining. To promote apoptosis, cirsilineol activates caspase-9, caspase-3, and PARP (poly ADP-ribose polymerase). Cirsilineol-induced loss of the mitochondrial membrane potential (MMP) brings a remarkable change and releases cytochrome-c to the cytosol. The induction of apoptosis via the mitochondrial pathway is the mode of action for the anti-proliferative activity of cirsilineol against cancerous cells. Moreover, cirsilineol is effective in ameliorating TNBS (tri-nitrobenzene sulfonic acid) -induced experimental colitis in mice, possibly because of its novel immunoregulatory activities with selective inhibitor IFN-γ/STAT1/T-bet signaling in the colonic lamina propria CD4 + T-cells particular for Crohn's disease [88].

Biological Activity of Jaceosidin
Regulation of the transcription activator and signal transducer (STAT 1) is being explored for the treatment of bowel diseases. However, few chemicals have been reported for the inhibition of STAT1/ IFN-g signaling for the treatment of Crohn's disease. A natural compound, cirsilineol, isolated from the A. vestita plant significantly ameliorated TNBS (trinitro-benzene sulphonic acid)-induced T-cell-mediated mice colitis. It is closely associated with reduced auto-reactive T-cell activation and proliferation. Moreover, the action of anti-inflammatory and pro-inflammatory cytokines with cirsilineol therapy was found to increase the regulatory T-cell activity and decrease the effector Th-1 cell activity, as characterized by the up-regulation of TGF-b and IL-10 and down-regulation of IFN-g. Importantly, in the presence of a higher level of IFN-g the inhibition by compound cirsilineol of STAT1/IFN-g signalling seems reversible, suggesting that compound cirsilineol might be a potential candidate for the treatment of human inflammatory T-cell-mediated bowel diseases [88]. The flavone jaceosidin isolated from A. vestita showed an antiproliferation effect on several human cancer cell lines. It significantly reduced SKOV-3, PC3, HeLa, and CAOV-3 cell proliferation in a concentration-dependent manner, whereas CAOV-3 showed time-dependent inhibition, and apoptosis increased in CAOV-3 cells. Flavone induced the cleavage of PARP (poly ADP-ribose polymerase) and caspase-3 and increased the cleaved caspase-9 levels. It also elevated the cytochrome c level in the cytosol, which shows the antitumor property of jaceosidin [87].

Conclusions
Herbal flora is gaining much attention from scientists for the development of strategies and to know the therapeutic potential of novel herbal constituents to treat various health disorders. The information regarding the use of A. vestita as a folkoric medicine was mostly confined to the native inhabitants.
The phenolic compounds present in the oil are responsible for the antioxidant activity, whereas flavones and sesquiterpenes exhibit anti-tumor and anti-inflammatory activity, respectively. The anti-inflammatory activity of A. vestita leaf extracts hhasave been time-tested.
A. vestita can also be a promising source of anti-COVID-19 remedies. The inverse correlation between the antiviral activity of artemisinin contents and total flavonoid contents is reported. Artemisinin singly or in combination with other components acts synergistically to block the post-entry viral infection. Moreover, essential oil and extract from the pre-and post-flowering stages of A. vestita have been reported to possess antifungal activity against phytopathogenic fungi and can be an effective drug against dermatophytes.
Although A. vestita has been used for the treatment of numerous ailments, standardization and extensive clinical studies shall facilitate optimizing the dose. Moreover, the plant is not yet extensively explored by the scientific community, as the successful reports related to the efficacy of its extracts are fragmentary and show variable results.
Research related to the development of A. vestita-based value-added products and the enhancement of the efficacy of the extracts by blending with other natural extracts is also required. Being a reservoir of phytochemicals, it is necessary to conserve this species for further research so as to gain the associated medicinal benefits for health security.