Vibrational Study on the Structure, Bioactivity, and Silver Adsorption of Silk Fibroin Fibers Grafted with Methacrylonitrile

Natural fibers have received increasing attention as starting materials for innovative applications in many research fields, from biomedicine to engineering. Bombyx mori silk fibroin has become a material of choice in the development of many biomedical devices. Grafting represents a good strategy to improve the material properties according to the desired function. In the present study, Bombyx mori silk fibroin fibers were grafted with methacrylonitrile (MAN) with different weight gains. The potential interest in biomedical applications of MAN functionalization relies on the presence of the nitrile group, which is an acceptor of H bonds and can bind metals. IR and Raman spectroscopy were used to characterize the grafted samples and the possible structural changes induced by grafting. Afterward, the same techniques were used to study the bioactivity (i.e., the calcium phosphate nucleation ability) of MAN-grafted silk fibroins after ageing in simulated body fluid (SBF) for possible application in bone tissue engineering, and their interaction with Ag+ ions, for the development of biomaterials with enhanced anti-microbial properties. MAN was found to efficiently polymerize on silk fibroin through polar amino acids (i.e., serine and tryptophan), inducing an enrichment in silk fibroin-ordered domains. IR spectroscopy allowed us to detect the nucleation of a thin calcium phosphate layer and the uptake of Ag+ ions through the nitrile group, which may foster the application of these grafted materials in biomedical applications.


Introduction
The functionalization of natural fibers has gained considerable interest among material scientists to create high-performing and environmentally friendly materials for traditional and innovative applications [1]. Natural fibers can be divided into two main groups according to their chemical composition: plant fibers based on polysaccharides (such as cotton or linen) and animal fibers based on proteins (i.e., silk or wool). Wool keratins and silk fibroins are biopolymers with outstanding properties that allow their application over a wide variety of fields, from biomedicine (as sutures [2], nanocarriers [3], hydrogels [4] or scaffolds [5]), to engineering (as electrical nanogenerators [6] or wearable electronic devices [7]). In recent years, silk fibroin has become a material of choice in the development of many biomedical devices [8][9][10].
Silk is produced by different species of insects (silkworms, such as B. mori) and spiders and is composed of proteins rich in glycine, serine, and alanine residues organized into α-helix or β-sheet nanocrystals embedded into a semi-amorphous matrix [11]. In particular, silk from B. mori is formed by two different proteins: fibroin, a semi-crystalline biopolymer, and sericin, a glue-like protein, which is usually removed by different degumming processes. While the former protein has shown biocompatibility comparable to commonly used biomaterials, the latter protein is responsible for adverse biological responses [12].

IR Spectra
The IR spectra in both the parallel and the perpendicular orientations (Figures 2, 3, S2 and S3, Supplementary Material) substantially confirmed the same conformational organization: Amide I showed a shoulder around 1697 cm −1 (anti-parallel β-sheet conformation) and a strong band centered at 1616 cm −1 (parallel mode) and 1624 cm −1 (perpendicular mode). Amide II and III fell at 1508 cm −1 (perpendicular) and 1515 cm −1 (parallel), and 1260 and 1230 cm −1 , respectively. All these spectral features revealed that silk fibroin had an overall β-sheet conformation [22,31]. The bands at 1000-976 cm −1 (parallel) and 996-975 cm −1 (perpendicular) are typical of Ala-Gly sequences in fibroin, which generate a close network of hydrogen bonds among fibroin chains, giving rise to the typical β-sheet crystalline structure of silk [20]. main bands assignable to β-sheet (β) or unordered (Un) conformation as well as to tyrosine (Y), phenylalanine (F), tryptophan (W), and the grafting agent (MAN) are indicated.

IR Spectra
The IR spectra in both the parallel and the perpendicular orientations (Figures 2, 3, S2 and S3, Supplementary Material) substantially confirmed the same conformational organization: Amide I showed a shoulder around 1697 cm −1 (anti-parallel β-sheet conformation) and a strong band centered at 1616 cm −1 (parallel mode) and 1624 cm −1 (perpendicular mode). Amide II and III fell at 1508 cm −1 (perpendicular) and 1515 cm −1 (parallel), and 1260 and 1230 cm −1 , respectively. All these spectral features revealed that silk fibroin had an overall β-sheet conformation [22,31]. The bands at 1000-976 cm −1 (parallel) and 996-975 cm -1 (perpendicular) are typical of Ala-Gly sequences in fibroin, which generate a close network of hydrogen bonds among fibroin chains, giving rise to the typical β-sheet crystalline structure of silk [20].  . IR spectra recorded in the parallel orientation on B. mori silk fibroin fibers before and after grafting with MAN at the highest weight gain (i.e., 60%) in the 3500-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges. The spectra are normalized to the intensity of the Amide I band (1616-1621 cm −1 ). Figure 2. IR spectra recorded in the parallel orientation on B. mori silk fibroin fibers before and after grafting with MAN at the highest weight gain (i.e., 60%) in the 3500-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges. The spectra are normalized to the intensity of the Amide I band (1616-1621 cm −1 ). The main bands assigned to β-sheet (β) conformation, as well as to alanine-glycine domains (Ala-Gly), are indicated together with those having a contribution from MAN. The main bands assigned to β-sheet (β) conformation, as well as to alanine-glycine domains (Ala-Gly), are indicated together with those having a contribution from MAN.
(a) (b) Figure 3. IR recorded in the perpendicular orientation on B. mori silk fibroin fibers before and after grafting with MAN at the highest weight gain (i.e., 60%) in the 3500-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges. The spectra are normalized to the intensity of the Amide I band (1624-1625 cm −1 ). The main bands assigned to β-sheet (β) conformation, as well as to alanine-glycine domains (Ala-Gly), are indicated together with those having a contribution from MAN.

Methacrylonitrile-Grafted
The Raman I 2237 /I Amide I and I 703 /I Amide I intensity ratios were the most suitable for spectroscopically monitoring grafting: the first band of each ratio is diagnostic of the grafting agent, while the Amide I band was chosen as an internal standard for silk, since it is not affected by the unreacted monomer ( Figure 4). An almost perfect linear correlation was established between both intensity ratios and weight gain (I 2237 /I Amide I : R 2 = 0.999; I 703 /I Amide I : R 2 = 0.992). On the other hand, Wallasch et al. have used the Raman CN stretching vibration at about 2235 cm −1 to quantify MAN [36]. spectra, the CN stretching mode was observed at 2237 cm . On the basis of this wavenumber position, we can exclude any possible hydrogen bond interaction with silk fibroin, as already observed in (acrylonitrile-co-methyl acrylate)-silk blends [37]. On the other hand, in the association between liquid acrylonitrile and Ni 2+ ion [38], similar wavenumber values were reported for "free" CN groups (around 2245 cm −1 ).
Similarly, Raman spectra excluded the possibility that nitrile could be transformed into imino-ether (R-C(-OR')=NH), forming a covalent bond with protein amino groups. This effect was previously observed in the immobilization of antibodies on electrografted polyMAN films, but only in basic conditions (pH > 9) [39], which differed from the acidic environment used in the grafting procedure (see Paragraph 3.1). Therefore, on the basis of our data, we may exclude the possibility that the nitrile group could be involved directly in the grafting mechanism.  The position and full width at half-maximum (FWHM) of the Amide I Raman band were poorly affected by grafting with MAN; FWHM differences were < 1 cm −1 , and therefore, they were not considered significant. The trend of the I1266/I1229 intensity ratio (i.e., between the bands of the Amide III spectral region sensitive to unordered and β-sheet conformations, respectively) showed a slight decrease from 0.44 ± 0.02 in the untreated silk to 0.36 ± 0.02 in the grafted fibers with weight gain of 60%, suggesting a moderate decrease in the unordered conformation content. The graph in Figure 5 shows that the I1266/I1229 intensity ratio is correlated with weight gain, suggesting that this effect progressively increased with the amount of grafted MAN. The position of the Raman CN stretching band could give insights into its H-bond state; the nitrile group can be the acceptor of hydrogen bonds (i.e., from water). In our spectra, the CN stretching mode was observed at 2237 cm −1 . On the basis of this wavenumber position, we can exclude any possible hydrogen bond interaction with silk fibroin, as already observed in (acrylonitrile-co-methyl acrylate)-silk blends [37]. On the other hand, in the association between liquid acrylonitrile and Ni 2+ ion [38], similar wavenumber values were reported for "free" CN groups (around 2245 cm −1 ).
Similarly, Raman spectra excluded the possibility that nitrile could be transformed into imino-ether (R-C(-OR')=NH), forming a covalent bond with protein amino groups. This effect was previously observed in the immobilization of antibodies on electrografted polyMAN films, but only in basic conditions (pH > 9) [39], which differed from the acidic environment used in the grafting procedure (see Paragraph 3.1). Therefore, on the basis of our data, we may exclude the possibility that the nitrile group could be involved directly in the grafting mechanism.
The position and full width at half-maximum (FWHM) of the Amide I Raman band were poorly affected by grafting with MAN; FWHM differences were < 1 cm −1 , and therefore, they were not considered significant. The trend of the I 1266 /I 1229 intensity ratio (i.e., between the bands of the Amide III spectral region sensitive to unordered and β-sheet conformations, respectively) showed a slight decrease from 0.44 ± 0.02 in the untreated silk to 0.36 ± 0.02 in the grafted fibers with weight gain of 60%, suggesting a moderate decrease in the unordered conformation content. The graph in Figure 5 shows that the I 1266 /I 1229 intensity ratio is correlated with weight gain, suggesting that this effect progressively increased with the amount of grafted MAN.
This trend was opposite to what was observed in HEMA and HBA-grafted B. mori fabrics [20] and MAA-grafted Tussah silk fibroin [19]: in the latter case, a rearrangement in the hydrogen bonds pattern of fibroin in crystalline domains due to grafting was proposed. Tsukada et al. previously observed [23] that the glass transition temperature of MAN-grafted fibers decreased since hydrogen bond rearrangement occurred only in the amorphous regions, further supporting our spectroscopic data. In order to gain more insight into the grafting mechanism, several Raman intensity ratios relative to the diagnostic bands of polar amino acids were investigated. The present study used experimental conditions that proved suitable to maximize the grafting yield: an initiator forms free radicals that may react with carboxyl, amine, and hydroxyl side groups of different amino acids. This process leads to forming a fibroin macroradical that reacts with the monomer, leading to the propagation of a grafted polymer chain. In particular, hydroxyl and amine groups in the side chains of serine, threonine, and tryptophan amino acids have been reported to interact with electron donor groups such as sulfate [31]. Previous studies identified the OH group of Ser and the NH group of glycine as the most accessible grafting sites of fibroin [20]. Guan et al. investigated the grafting of a vinyl phosphate (diethyl-2-(methacryloyloxyethyl) phosphate) to silk fibroin: the quantitative analysis of amino acids before and after grafting showed that cysteine, serine, and tyrosine reacted with potassium persulfate (the initiator) creating the RCH2 • and RCH2S • radicals, i.e., the reaction sites for grafting [40]. Pourjavadi et al. reported the involvement of secondary alcohols in grafting MAN on chitosan, using ammonium persulfate as an initiator, which can extract H from those groups [41].
The involvement of serine residues in the grafting mechanism of silk fibroins with acrylate monomers has been extensively investigated [19,21,22,31] using the intensity of the 1404 cm −1 band (bending vibration of serine hydroxyl group), which reduced upon grafting. In MAN-grafted samples, the I1404/IAmide I Raman intensity ratio increased (in the range of 0.26-0.31) compared to the untreated sample (I1404/IAmide I = 0.20 ± 0.04), as expectable based on the contribution of the CH2 bending vibration of grafted MAN (1405 cm −1 , Figure 1). However, this ratio does not linearly increase with the MAN content ( Figure S4, Supplementary Material); we interpreted this behavior by hypothesizing that simultaneously, the contribution of the δOH mode of serine decreased due to the involvement of this amino acid in grafting. This trend was opposite to what was observed in HEMA and HBA-grafted B. mori fabrics [20] and MAA-grafted Tussah silk fibroin [19]: in the latter case, a rearrangement in the hydrogen bonds pattern of fibroin in crystalline domains due to grafting was proposed. Tsukada et al. previously observed [23] that the glass transition temperature of MAN-grafted fibers decreased since hydrogen bond rearrangement occurred only in the amorphous regions, further supporting our spectroscopic data.
In order to gain more insight into the grafting mechanism, several Raman intensity ratios relative to the diagnostic bands of polar amino acids were investigated. The present study used experimental conditions that proved suitable to maximize the grafting yield: an initiator forms free radicals that may react with carboxyl, amine, and hydroxyl side groups of different amino acids. This process leads to forming a fibroin macroradical that reacts with the monomer, leading to the propagation of a grafted polymer chain. In particular, hydroxyl and amine groups in the side chains of serine, threonine, and tryptophan amino acids have been reported to interact with electron donor groups such as sulfate [31]. Previous studies identified the OH group of Ser and the NH group of glycine as the most accessible grafting sites of fibroin [20]. Guan et al. investigated the grafting of a vinyl phosphate (diethyl-2-(methacryloyloxyethyl) phosphate) to silk fibroin: the quantitative analysis of amino acids before and after grafting showed that cysteine, serine, and tyrosine reacted with potassium persulfate (the initiator) creating the RCH 2 • and RCH 2 S • radicals, i.e., the reaction sites for grafting [40]. Pourjavadi et al. reported the involvement of secondary alcohols in grafting MAN on chitosan, using ammonium persulfate as an initiator, which can extract H from those groups [41].
The involvement of serine residues in the grafting mechanism of silk fibroins with acrylate monomers has been extensively investigated [19,21,22,31] using the intensity of the 1404 cm −1 band (bending vibration of serine hydroxyl group), which reduced upon grafting. In MAN-grafted samples, the I 1404 /I Amide I Raman intensity ratio increased (in the range of 0.26-0.31) compared to the untreated sample (I 1404 /I Amide I = 0.20 ± 0.04), as expectable based on the contribution of the CH 2 bending vibration of grafted MAN (1405 cm −1 , Figure 1). However, this ratio does not linearly increase with the MAN content ( Figure S4, Supplementary Material); we interpreted this behavior by hypothesizing that simultaneously, the contribution of the δOH mode of serine decreased due to the involvement of this amino acid in grafting.
The 1555 cm −1 band is attributed to the indole ring vibration of tryptophan: the linear decrease of the I 1555 /I Amide I Raman intensity ratio with MAN content may confirm the involvement of Trp residues in grafting ( Figure S5, Supplementary Material).

Figure 6.
Trend of the IR A1217/A1230 absorbance ratio (obtained from the parallel and perpendicular spectra) as a function of the weight gain (i.e., MAN content w/w%).
The above-mentioned bands are typical of the polymer, while the strongest band of the monomer at about 940 cm −1 was not detected. The absence or shallow content of unreacted monomers was also described by Pourjavadi et al. about the grafting of MAN on chitosan, using ammonium persulfate as an initiator [41].
The IR spectra of the grafted samples confirmed the overall β-sheet structure by the presence of the 1697 cm −1 band (anti-parallel β-sheet) in all grafted samples, most evident Figure 6. Trend of the IR A 1217 /A 1230 absorbance ratio (obtained from the parallel and perpendicular spectra) as a function of the weight gain (i.e., MAN content w/w%).
The above-mentioned bands are typical of the polymer, while the strongest band of the monomer at about 940 cm −1 was not detected. The absence or shallow content of unreacted monomers was also described by Pourjavadi et al. about the grafting of MAN on chitosan, using ammonium persulfate as an initiator [41].
The IR spectra of the grafted samples confirmed the overall β-sheet structure by the presence of the 1697 cm −1 band (anti-parallel β-sheet) in all grafted samples, most evident in the perpendicular orientation ( Figure 3), as well as by the components at 996 and 975 cm −1 , identified as marker bands of Ala-Gly sequences of silk fibroin [42].
IR spectra allowed us to gain more insights into the structural changes caused by grafting and get information on the orientation degree of the fibers. The last parameter could be qualitatively evaluated by inspecting the IR spectra recorded by placing the fibers along one specific direction (parallel) and perpendicularly to it. Although the prevailing conformation remained β-sheet upon grafting, minor shifts were observed: in particular, all the Amide bands shifted towards higher wavenumbers. In the parallel orientation, going from the untreated fibers to the MAN 60% grafted sample, Amide A, Amide I, II, and III bands shifted from 3278, 1616, 1515, and 1260 cm −1 to 3282, 1621, 1516, and 1262 cm −1 , respectively ( Figure 2); analogous shifts were observed in the perpendicular orientation ( Figure 3). All these shifts could be explained by considering that hydrogen bonds between adjacent silk chains weakened due to grafting, in agreement with what was observed with MAA grafting [19].
Upon grafting, the IR spectra evidenced structural rearrangements in B. mori silk fibroin towards a decreased disordered conformation, as can be seen from Figures S2 and S3, Supplementary Material, at increasing grafting, Amide I and II progressively narrowed, and Amide II appeared to weaken with respect to Amide I. The latter trend appeared more evident in the spectra recorded in the parallel orientation ( Figure S2, Supplementary Material). Accordingly, Figures 7 and 8 show that the FWHM of Amide I and II, the A Amide I /A Amide II ratio (an index of structural regularity), and the Ω Amide I/Amide II marker (orientational order parameter) correlated well with the MAN content in the fibers.
in the perpendicular orientation (Figure 3), as well as by the components at 996 and 975 cm −1 , identified as marker bands of Ala-Gly sequences of silk fibroin [42].
IR spectra allowed us to gain more insights into the structural changes caused by grafting and get information on the orientation degree of the fibers. The last parameter could be qualitatively evaluated by inspecting the IR spectra recorded by placing the fibers along one specific direction (parallel) and perpendicularly to it. Although the prevailing conformation remained β-sheet upon grafting, minor shifts were observed: in particular, all the Amide bands shifted towards higher wavenumbers. In the parallel orientation, going from the untreated fibers to the MAN 60% grafted sample, Amide A, Amide I, II, and III bands shifted from 3278, 1616, 1515, and 1260 cm −1 to 3282, 1621, 1516, and 1262 cm −1 , respectively ( Figure 2); analogous shifts were observed in the perpendicular orientation ( Figure 3). All these shifts could be explained by considering that hydrogen bonds between adjacent silk chains weakened due to grafting, in agreement with what was observed with MAA grafting [19].
Upon grafting, the IR spectra evidenced structural rearrangements in B. mori silk fibroin towards a decreased disordered conformation, as can be seen from Figures S2 and S3, Supplementary Material, at increasing grafting, Amide I and II progressively narrowed, and Amide II appeared to weaken with respect to Amide I. The latter trend appeared more evident in the spectra recorded in the parallel orientation ( Figure S2, Supplementary Material). Accordingly, Figures 7 and 8 show that the FWHM of Amide I and II, the AAmide I/AAmide II ratio (an index of structural regularity), and the ΩAmide I/Amide II marker (orientational order parameter) correlated well with the MAN content in the fibers.   In the Amide III range, the A1230/A1260 was 1.66 in the silk fibroin untreated fibers and decreased to about 1.5 in all the grafted samples, with no significant differences between each other ( Figure S7, Supplementary Material). Surprisingly, the grafting with only 10% MAN appeared to induce a change in the ratio and alter the silk fibroin conformation to some extent. According to Bhat and Nadiger [43], the trend of this ratio confirms that upon grafting, structural rearrangements towards a more ordered state occurred.
The ΩAmide III orientational parameter showed no correlation with weight gain and no significant changes going from untreated silk fibroin fibers to MAN-grafted ones ( Figure  S8, Supplementary Material). This result was in agreement with previous studies on B. mori silk fibers grafted with MAN, which evidenced that the molecular orientation in the crystalline region of fibroin, investigated by the XRD technique, remains relatively unchanged after grafting [23,24]. At the same time, a previous study on similar grafted materials found a lower material's birefringence, suggesting that most of the physical changes occurred in the amorphous regions, where vinyl monomers can more easily penetrate and bind to silk reactive sites [24]. On the other hand, neither MAA-grafted B. mori [22] nor Tussah [19] silk fibroin fibers showed any orientational rearrangements induced by grafting. In the Amide III range, the A 1230 /A 1260 was 1.66 in the silk fibroin untreated fibers and decreased to about 1.5 in all the grafted samples, with no significant differences between each other ( Figure S7, Supplementary Material). Surprisingly, the grafting with only 10% MAN appeared to induce a change in the ratio and alter the silk fibroin conformation to some extent. According to Bhat and Nadiger [43], the trend of this ratio confirms that upon grafting, structural rearrangements towards a more ordered state occurred.
The Ω Amide III orientational parameter showed no correlation with weight gain and no significant changes going from untreated silk fibroin fibers to MAN-grafted ones ( Figure S8, Supplementary Material). This result was in agreement with previous studies on B. mori silk fibers grafted with MAN, which evidenced that the molecular orientation in the crystalline region of fibroin, investigated by the XRD technique, remains relatively unchanged after grafting [23,24]. At the same time, a previous study on similar grafted materials found a lower material's birefringence, suggesting that most of the physical changes occurred in the amorphous regions, where vinyl monomers can more easily penetrate and bind to silk reactive sites [24]. On the other hand, neither MAA-grafted B. mori [22] nor Tussah [19] silk fibroin fibers showed any orientational rearrangements induced by grafting. Table S1 shows the weight gain measured after ageing of the fibers in the Ag +containing solution for 48 h. As can be easily seen, the sample containing the highest amount of MAN underwent the highest weight gain (3.8%) under these conditions; moreover, it must be noticed that also the silk fibroin control sample underwent a significant weight gain (2.8%). According to a previous study [29], the Ag + uptake by silk fibroin may be explained by considering that the most probable binding sites in the protein backbone are the free carboxyl groups of aspartic and glutamic acid occurring in the amorphous polypeptide sequences. Since these acidic groups have a pKa of about 4-4.8, they are almost completely dissociated at pH 7.4, thus providing negatively charged groups available for binding metal cations [29]. Figure 9 shows that the % weight gain of the fibers due to Ag + uptake linearly increased with the MAN content. In other words, the samples containing the highest amounts of MAN were able to adsorb higher amounts of silver.

Silver Adsorption Tests
Table S1 shows the weight gain measured after ageing of the fibers in the Ag + -containing solution for 48 h. As can be easily seen, the sample containing the highest amount of MAN underwent the highest weight gain (3.8%) under these conditions; moreover, it must be noticed that also the silk fibroin control sample underwent a significant weight gain (2.8%). According to a previous study [29], the Ag + uptake by silk fibroin may be explained by considering that the most probable binding sites in the protein backbone are the free carboxyl groups of aspartic and glutamic acid occurring in the amorphous polypeptide sequences. Since these acidic groups have a pKa of about 4-4.8, they are almost completely dissociated at pH 7.4, thus providing negatively charged groups available for binding metal cations [29]. Figure 9 shows that the % weight gain of the fibers due to Ag + uptake linearly increased with the MAN content. In other words, the samples containing the highest amounts of MAN were able to adsorb higher amounts of silver. Figure 9. Trend of the % weight gain of the fibers due to Ag + uptake as a function of the MAN content (w/w%). The silk fibroin control sample is indicated with a red square because it was not used to calculate the regression line. Figures 10 and 11 show the IR spectra of the 60% MAN-grafted fibers before and after ageing for 48 h in the Ag + solution, recorded in the parallel and perpendicular orientations, respectively. Due to the brownish color of the aged fibers, the recorded Raman spectra were not of good quality and, thus, are not reported and discussed. Figure 9. Trend of the % weight gain of the fibers due to Ag + uptake as a function of the MAN content (w/w%). The silk fibroin control sample is indicated with a red square because it was not used to calculate the regression line. Figures 10 and 11 show the IR spectra of the 60% MAN-grafted fibers before and after ageing for 48 h in the Ag + solution, recorded in the parallel and perpendicular orientations, respectively. Due to the brownish color of the aged fibers, the recorded Raman spectra were not of good quality and, thus, are not reported and discussed.
As can be easily seen, upon ageing, the CN stretching at 2235 cm −1 slightly shifted to a higher wavenumber value in both the parallel and perpendicular spectra (Figures 10 and 11). No analogous changes were observed in the other grafted samples. A similar shift towards higher wavenumbers was reported for the interaction between polyMAN and lithium salts [44], as well as between nitriles and Ag + and other metals [38,45]. Moreover, Zarembowitch and Maleki [32] have reported that upon Cu + complexation by MAN, the CN stretching band shifted to higher wavenumbers. The frequency shift was explained by these authors by considering that copper (I) is bound to the nitrogen atom of the CN group and a strengthening of the σ C≡N system occurs; however, the existence of a relatively strong π back-bonding from copper(I) to nitrogen was revealed. In agreement with the literature, we may interpret our results as a sign of silver chelation.
In all the samples, the MAN content remained constant upon ageing, as revealed by the constancy of the A 1217 /A 1230 absorbance ratio ( Figure S9, Supplementary Material). In other words, no MAN release in the ageing medium was detected under the used experimental conditions.
In the parallel spectra of the 60% MAN-grafted fibers, the Amide I and II bands slightly shifted to higher wavenumbers ( Figure 10) upon ageing; the former band also broadened, while such behavior was not detected for Amide II ( Figure S10, Supplementary Material). EDTA-modified B. mori silk fibroin fibers showed a similar shift towards higher wavenumbers for both Amide I and Amide II bands after binding to Cu 2+ and Co 2+ ions [46]. In the perpendicular spectra, both Amide I and II bands broadened upon silver uptake (Figures 11 and S11, Supplementary Material). The other grafted samples showed less significant changes, as expectable on the basis of their lower silver uptake ( Figures S10 and S11, Supplementary Material); the silk fibroin control fibers showed a significant broadening of Amide I in the parallel spectra ( Figure S10a  (b) Figure 10. IR spectra recorded in the parallel orientation on the 60% MAN-grafted fibers before and after ageing for 48 h in the Ag + solution in the 2280-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges.

(a)
Molecules 2023, 28, x FOR PEER REVIEW 13 of 27 (b) Figure 11. IR spectra recorded in the parallel orientation on the 60% MAN-grafted fibers before and after ageing for 48 h in the Ag + solution in the 2280-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges.
As can be easily seen, upon ageing, the CN stretching at 2235 cm −1 slightly shifted to a higher wavenumber value in both the parallel and perpendicular spectra (Figures 10  and 11). No analogous changes were observed in the other grafted samples. A similar shift towards higher wavenumbers was reported for the interaction between polyMAN and lithium salts [44], as well as between nitriles and Ag + and other metals [38,45]. Moreover, Zarembowitch and Maleki [32] have reported that upon Cu + complexation by MAN, the CN stretching band shifted to higher wavenumbers. The frequency shift was explained by these authors by considering that copper (I) is bound to the nitrogen atom of the CN group and a strengthening of the σ C≡N system occurs; however, the existence of a relatively strong π back-bonding from copper(I) to nitrogen was revealed. In agreement with the literature, we may interpret our results as a sign of silver chelation.
In all the samples, the MAN content remained constant upon ageing, as revealed by the constancy of the A1217/A1230 absorbance ratio ( Figure S9, Supplementary Material). In other words, no MAN release in the ageing medium was detected under the used experimental conditions.
In the parallel spectra of the 60% MAN-grafted fibers, the Amide I and II bands slightly shifted to higher wavenumbers ( Figure 10) upon ageing; the former band also broadened, while such behavior was not detected for Amide II ( Figure S10, Supplementary Material). EDTA-modified B. mori silk fibroin fibers showed a similar shift towards higher wavenumbers for both Amide I and Amide II bands after binding to Cu 2+ and Co 2+ ions [46]. In the perpendicular spectra, both Amide I and II bands broadened upon silver Figure 11. IR spectra recorded in the perpendicular orientation on the 60% MAN-grafted fibers before and after ageing for 48 h in the Ag + solution in the 2280-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges.
In the Amide III range, the A 1230 /A 1260 absorbance ratio decreased in both parallel and perpendicular spectra of the 60% MAN-grafted sample upon ageing ( Figure S12, Supplementary Material). The other grafted samples showed less significant changes, as expectable on the basis of their lower silver uptake; the control fibers showed a significant variation of the ratio in the perpendicular spectra, which generally showed more significant changes.
The graphs reported in Figure S13a, Supplementary Material, showed that the broadening of Amide I (in the parallel spectra) and Amide II (in the perpendicular spectra) increased at increasing silver uptake. In particular, by excluding the 25% MAN-grafted sample (outlier) from the graphs, a very good linear trend was observed between the % variation in the FWHM of Amide I and Amide II and % weight gain (i.e., Ag uptake), with R 2 values as high as 0.9499 ( Figure S13b, Supplementary Material).
Upon ageing, the A Amide I /A Amide II ratio decreased in the parallel spectra of the 60% MAN-grafted fibers while increased in the perpendicular ones ( Figure S14, Supplementary Material). Similar behavior was also observed for the other aged samples, particularly for the silk fibroin control fibers. The changes in the perpendicular spectra appeared more significant than those in the parallel ones, and a good linear trend was observed (R 2 = 0.8866) between the % variation in the A Amide I /A Amide II and A 1230 /A 1260 ratios in perpendicular spectra and % weight gain (i.e., Ag uptake), by excluding the 25% MAN-grafted sample outlier ( Figure S15a, Supplementary Material). An analogously good correlation was found between Ω Amide I/Amide II ( Figure S16, Supplementary Material) and % weight gain due to Ag uptake ( Figure S15b, Supplementary Material). A similar A Amide I /A Amide II trend was observed after the binding of Co 2+ ions on EDTA and tannic acid-modified silk fibroins, while the binding of Cu 2+ ions showed an opposite trend, suggesting that the metal-fiber interactions are strongly dependent on the kind of fiber and the metal cation [46].
The reported results evidenced Ag + chelation and structural rearrangements in silk fibroin, which were found to correlate with silver uptake.

Bioactivity Tests in SBF 2.4.1. Raman Spectra
After 7 days of immersion in the SBF solution, poor weight variations in all samples (either untreated or grafted) were measured, ranging from negative values (−1.5% in the control sample, corresponding to a weight loss) to slightly positive values (+1% in MAN 40% and MAN 60% samples), as reported in Table S2, Supplementary Material. Based on these data, only these last two samples (with the control as a reference) were immersed in the SBF solution for up to 28 days to study their bioactivity at longer times. After 28 days in SBF, only the MAN 60% sample showed a moderate weight gain (Table S2, Supplementary Material), while the control and MAN 40% showed a weight loss ranging from 4 to 6%. Based on the data reported in Table S2, Supplementary Material, it appears that the presence of the grafted polymer protected the fibers against weight loss. In other words, the higher the MAN content, the lower the weight loss: the data obtained at 7 days support a linear correlation between weight gain after immersion in the SBF solution and MAN content % (Figure 12), while, due to the reduced number of samples, the correlation is less robust at 28 days of immersion.  Figure 13 shows the Raman spectra of silk fibroin control and grafted fibers (MAN 40% and MAN 60% samples) before and after immersion in SBF for 7 and 28 days.  Figure 13 shows the Raman spectra of silk fibroin control and grafted fibers (MAN 40% and MAN 60% samples) before and after immersion in SBF for 7 and 28 days. Figure 12. Trend of the % weight gain of the fibers upon ageing in SBF for 7 and 28 days as a function of the MAN content (w/w%). Figure 13 shows the Raman spectra of silk fibroin control and grafted fibers (MAN 40% and MAN 60% samples) before and after immersion in SBF for 7 and 28 days. Raman spectroscopy could not detect any nucleation of an inorganic phase even on the samples having positive weight gains, since the technique is more sensitive to the bulk of the material [30]. However, it proved suitable to determine whether the samples' weight variation was associated with a mass loss due to the grafting agent. This was possible thanks to the previously mentioned Raman I2237/IAmide I and I703/IAmide I intensity ratios (Figure 4), which were found to be highly correlated with weight gain. Figures S17 and Raman spectroscopy could not detect any nucleation of an inorganic phase even on the samples having positive weight gains, since the technique is more sensitive to the bulk of the material [30]. However, it proved suitable to determine whether the samples' weight variation was associated with a mass loss due to the grafting agent. This was possible thanks to the previously mentioned Raman I 2237 /I Amide I and I 703 /I Amide I intensity ratios (Figure 4), which were found to be highly correlated with weight gain. Figures S17 and S18, Supplementary Material, report the trends of the I 2237 /I Amide I and I 703 /I Amide I intensity ratios before and after immersion of the fibers in the SBF solution for 7 and 28 days. The regression lines reported in Figure 4 allowed us to estimate the MAN content % of the grafted samples before and after the bioactivity tests in SBF (Table 1). Both intensity ratios revealed that none of the samples showed any significant variation in the MAN content % after 7 days of immersion in SBF; therefore, at this stage, no loss of the grafted polymer occurred. On the contrary, both ratios agreed about a significant loss of the grafting agent after 28 days of immersion in the SBF solution: both grafted samples showed a reduction of the above-mentioned intensity ratios by about 12-14% (Figures S17 and S18, Supplementary Material), which corresponded to an effective reduction of MAN content by about 5-7% (Table 1). The Raman spectroscopic parameters, already discussed for the samples before the bioactivity tests, were poorly affected by immersion in the SBF solution ( Figure 13). In particular, the position and the FWHM of the Amide I band did not change. The same result was observed after 7 days of immersion in SBF of silk fibroin grafted with phosphorylated methacrylates [30]. The trend of the I 1266 /I 1229 intensity ratio ( Figure S19, Supplementary Material) suggested the occurrence of little structural changes after 7 days of immersion. After 28 days of ageing, the I 1266 /I 1229 intensity ratio increased by 50% for MAN 40%, and 15% for MAN 60%, suggesting an increase in structural disorder of the fibers, which was not detected in control fibers. This result could appear unexpected, since previous studies on the proteolytic degradation of silk fibroin fibers reported an increase in structural order (revealed by the narrowing of the Raman Amide I band) attributed to the preferential degradation of amorphous domains [47]. The opposite trend observed in the present study may be explained in relation to the loss of the grafted MAN polymer: as reported above, its introduction in the fibers had determined an increase in order (see Paragraph 2.2); thus, it is not surprising that when it is removed, the degree of disorder increased., no changes in the I 1266 /I 1229 intensity ratio were detected in the control silk fibroin (i.e., no grafted) fibers.
Raman intensity ratios of the amino acids involved in the grafting reaction were more affected by the bioactivity tests: the I 1555 /I Amide I ratio, sensitive to tryptophan ( Figure S20, Supplementary Material), generally decreased even after 7 days of immersion. Opposite to what observed for the structural disorder, the control sample showed a higher decrease (−58% after 28 days) than the grafted samples (−40% and −28% after 28 days for MAN 40% and MAN 60% samples, respectively), further strengthening the idea that the grafted MAN polymer has a "protective role" against weight loss. The intensity decrease of Trp bands has been previously reported during the proteolytic degradation of Tussah silk fibers [47] and the alkaline hydrolysis of B. mori silk fibroin grafted with MAA [22]. This reduction was attributed to bulky Trp residues in the amorphous domains.
The I 1404 /I Amide I intensity ratio ( Figure S21, Supplementary Material), diagnostic for serine, showed a similar trend: an overall reduction after 7 days, followed by a more prominent reduction after 28 days. For this intensity ratio, the reduction was similar in control and grafted samples and ranged between 30 and 39%.
The I 850 /I 830 intensity ratio ( Figure S22, Supplementary Material), describing the Hbonding state of tyrosine, was the least affected by the immersion in SBF: after 28 days, control and grafted samples showed a minimal decrease by about 7-9%. A more marked decrease in this intensity ratio was observed during the proteolytic degradation of Tussah silk fibers [47]: the effect was explained by the cleavage of exposed Tyr residues.
The partial fiber degradation detected by Raman spectroscopy (with loss of MAN and amino acid residues) suggests that the weight gain results reported in Table S2, Supplementary Material, represent the sum of the contributions from two opposite phenomena, i.e., mass loss due to fiber degradation and mass gain due to calcium phosphate nucleation. The latter process was investigated by IR spectroscopy.

IR Spectra
IR spectra allowed us to gain more insights into the possible occurrence of calcium phosphate deposition, being sensitive to the surface of the samples (i.e., their first two microns). As an example, Figures 14 and 15 show the IR spectra recorded in the parallel and perpendicular orientations on the 60% MAN-grafted fibers before and after ageing in SBF for 28 days.
In agreement with a previous study [30], the A 1060 /A Amide I absorbance ratio was calculated to evaluate the nucleation of a calcium phosphate phase; in fact, near 1000 cm −1 , the phosphate antisymmetric stretching has been reported to fall [48]. The values of this spectroscopic marker are reported in Figure 16.
As can be easily seen from the spectra reported in Figures 14 and 15, the 1060 cm −1 band underwent a slight increase in relative intensity, and accordingly, the A 1060 /A Amide I absorbance ratio increased as well ( Figure 16); the changes appeared more significant in the perpendicular spectra and suggested the deposition of a thin calcium phosphate layer. The % variation in the A 1060 /A Amide I absorbance ratio upon ageing in SBF for 28 days was found to correlate with the weight gain of the samples after this treatment ( Figure S23, Supplementary Material). The formation of a calcium phosphate layer on silk fibroin can be explained by the interaction of calcium ions with the carboxylic residues of amino acids with a minor contribution of hydroxyl and carbonyl groups [48]. These types of interaction explained the extremely rapid mineralization upon immersion in the SBF solution of silk fibroin functionalized with anionic polypeptides, in which negative carboxylate ions acted as nucleation sites for the apatitic phase [5]. These results were of paramount importance in explaining the proliferation and adhesion of mesenchymal stem cells on the materials and, thus, their biocompatibility.
Upon ageing, the CN stretching at 2235 cm −1 did not show any significant change in its wavenumber value (Figures 14 and 15), suggesting that the CN group did not chelate calcium ions.
At IR spectroscopy, the MAN content remained constant upon ageing for 28 days, as revealed by the constancy of the A 1217 /A 1230 absorbance ratio ( Figure S24, Supplementary  Material). This result could appear in contrast with the Raman findings. In fact, it must be recalled that the two techniques have different sampling areas: Raman spectroscopy is sensitive to the sample bulk, while IR spectroscopy is sensitive to the sample surface. Therefore, IR spectroscopy revealed that no MAN release in the ageing medium occurred from the sample surface, while the MAN content of the sample bulk was affected by the ageing in SBF.
In agreement with the Raman findings, in the parallel spectra of the 60% MAN-grafted fibers (Figure 14), Amide I broadened upon ageing, while Amide II did not ( Figure S25, Supplementary Material); an analogous behavior was already observed for the samples aged in the Ag + -containing solution (Paragraph 2.3). No changes were observed in the FWHM of Amide I and II bands in the perpendicular spectra ( Figure 15).
In the parallel spectra of the 60% MAN-grafted fibers (Figure 14), the A Amide I /A Amide II absorbance ratio slightly decreased ( Figure S26, Supplementary Material), while it increased in the perpendicular ones (Figures 15 and S26, Supplementary Material), as already observed for the samples aged in the Ag + -containing solution (Paragraph 2.3). Changes in this ratio were also observed in dentin collagen upon calcium loss or chelation (i.e., demineralization or remineralization) [49]. Accordingly, the Ω Amide I/Amide II also had an analogous decrease ( Figure Figure S27 Figure 15. IR spectra recorded in the perpendicular orientation on the 60% MAN-grafted fibers before and after ageing for 28 days in SBF in the 2280-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges.
In agreement with a previous study [30], the A1060/AAmide I absorbance ratio was calculated to evaluate the nucleation of a calcium phosphate phase; in fact, near 1000 cm −1 , the phosphate antisymmetric stretching has been reported to fall [48]. The values of this spectroscopic marker are reported in Figure 16.  Figure 15. IR spectra recorded in the perpendicular orientation on the 60% MAN-grafted fibers before and after ageing for 28 days in SBF in the 2280-2200 cm −1 (a) and 1720-900 cm −1 (b) spectral ranges.
In agreement with a previous study [30], the A1060/AAmide I absorbance ratio was calculated to evaluate the nucleation of a calcium phosphate phase; in fact, near 1000 cm −1 , the phosphate antisymmetric stretching has been reported to fall [48]. The values of this spectroscopic marker are reported in Figure 16. Additionally, the Amide III region underwent the same variations observed upon silver uptake, i.e., a general decrease of the A 1230 /A 1260 absorbance ratio ( Figure S28, Supplementary Material). As observed upon immersion in the Ag + -containing solution, the % variation in this ratio correlates well with weight gain upon ageing in SBF ( Figure S29, Supplementary Material).
The observed trends allowed us to conclude that the chelation of silk fibroin with calcium and silver ions induced similar changes in the spectroscopic markers and, thus, in protein conformation. The presence of the grafted MAN polymer favored both processes. As a difference, the CN groups were found to participate in silver chelation, while no clear sign of this mechanism for calcium was inferred. Molecules 2023, 28, x FOR PEER REVIEW 20 of 27 Figure 16. Values of the A1060/AAmide I absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in SBF.
As can be easily seen from the spectra reported in Figures 14 and 15, the 1060 cm −1 band underwent a slight increase in relative intensity, and accordingly, the A1060/AAmide I absorbance ratio increased as well ( Figure 16); the changes appeared more significant in the perpendicular spectra and suggested the deposition of a thin calcium phosphate layer. The % variation in the A1060/AAmide I absorbance ratio upon ageing in SBF for 28 days was found to correlate with the weight gain of the samples after this treatment ( Figure S23, Supplementary Material). The formation of a calcium phosphate layer on silk fibroin can be explained by the interaction of calcium ions with the carboxylic residues of amino acids with a minor contribution of hydroxyl and carbonyl groups [48]. These types of interaction explained the extremely rapid mineralization upon immersion in the SBF solution of silk fibroin functionalized with anionic polypeptides, in which negative carboxylate ions acted as nucleation sites for the apatitic phase [5]. These results were of paramount importance in explaining the proliferation and adhesion of mesenchymal stem cells on the materials and, thus, their biocompatibility.
Upon ageing, the CN stretching at 2235 cm −1 did not show any significant change in its wavenumber value (Figures 14 and 15), suggesting that the CN group did not chelate calcium ions.
At IR spectroscopy, the MAN content remained constant upon ageing for 28 days, as revealed by the constancy of the A1217/A1230 absorbance ratio ( Figure S24, Supplementary Material). This result could appear in contrast with the Raman findings. In fact, it must be recalled that the two techniques have different sampling areas: Raman spectroscopy is sensitive to the sample bulk, while IR spectroscopy is sensitive to the sample surface. Therefore, IR spectroscopy revealed that no MAN release in the ageing medium occurred from the sample surface, while the MAN content of the sample bulk was affected by the ageing in SBF.

Materials
B. mori silk fibers were degummed before grafting and then treated with a mixture containing 2.5% sodium persulfate (the initiator), 2 mL/L formic acid (85%), 12% nonionic surfactant, and various amounts of methacrylonitrile, as previously described [23]. The mixture was heated at room temperature to 80 • C for 20 min and then maintained at the same temperature for 40 min. At the end of the reaction, the samples were washed with water. Fibers were extracted with a 1 g/L sodium hydrosulfate solution containing 1 mL/L nonionic surfactant (Noigen EC, Dai-ichi Kogyo Seiyaku Co., Tokyo, Japan) at 70 • C for 20 min to remove the unreacted MAN and washed. Silk fibers were dried in a forced draft at 100-105 • C for 2 h, placed in a desiccator over silica gel for 30 min, and weighed. The weight gain of silk fibers treated with MAN was calculated based on the oven-dried weights of the sample before and after the treatment: silk fibers with a weight gain of 10, 25, 32.5, 40, and 60% were prepared, the latter after 4 h of reaction time.

Silver Adsorption Tests
The adsorption and binding of silver cations onto untreated and grafted silk fibers were evaluated after immersing the samples in aqueous solutions of 0.5 mM AgNO 3 and KNO 3 at 37 • C for 48 h, as described elsewhere [29]. The solution pH of 7.4 was adjusted using ammonia.
The samples were then washed thoroughly with water, air-dried, and weighed in order to calculate the % weight gain according to the equation: % weight gain = 100 (w 48 − w 0 )/w 0 where w 48 is the dry weight after 48 h of immersion in the Ag + -containing solutions and w 0 is the initial weight.

Bioactivity Tests in SBF
The bioactivity of control and grafted silk fibroin fibers (test samples of 25 ± 5 mg) was assessed by immersing the samples into a simulated body fluid (SBF) buffered at pH 7.4 at 37 • C and containing the concentrations of the following ions: 142 mM Na + , 4 mM K + , 2.5 mM Ca 2+ , 148.8 mM Cl − , 4.2 mM HCO 3 − , 1 mM HPO 4 −2 [50]. The samples were then washed thoroughly with water, air-dried, and weighed in order to calculate the % weight gain according to the equation: % weight gain = 100 (w t − w 0 )/w 0 where w t is the dry weight after 7 or 28 days of immersion in SBF and w 0 is the initial weight.

Vibrational Spectroscopy
Raman and IR spectroscopy allowed the fibers' characterization before and after grafting.
Raman spectra were recorded on a Bruker MultiRam FT-Raman spectrometer equipped with a cooled Ge-diode detector. The excitation source was an Nd 3+ -YAG laser (1064 nm) in the backscattering (180 • ) configuration, with a power of 100 mW. The focused laser beam diameter was about 100 µm, and the spectral resolution was 4 cm −1 . The presented spectra are the average of three replicates: each replicate is the average of 10,000 measures. IR spectra were recorded on a Shimadzu IRTracer-100 FT-IR spectrometer, equipped with a QATR-10 single crystal diamond Attenuated Total Reflectance (ATR) accessory and a Deuterated Lanthanum α-Alanine doped TriGlycine Sulphate (DLaTGS) detector; the spectral resolution was 4 cm −1 with 64 scans for each spectrum. Fibers are intrinsically oriented samples; thus, their vibrational spectra depend on fiber orientation. Consequently, silk fibers were placed in the sample compartment keeping the same orientation for all the samples. The IR spectra were recorded in triplicate by positioning the fibers along one specific direction (conventionally termed "parallel") and perpendicularly to it. Recording fibers' spectra in different orientations allowed us to calculate some spectral parameters indicating orientational order, namely Ω Amide III and Ω Amide I/Amide II ; they were calculated as follows: Ω Amide III = X perp /X par Ω Amide I/Amide II = R par /R perp where X perp and X par were the A 1230 /A 1260 absorbance ratios (as peak heights) calculated from the perpendicular and parallel spectra, respectively, and R par and R perp were the A Amide I /A Amide II intensity ratios (as peak heights) calculated from the parallel and perpendicular spectra, respectively [21].

Conclusions
In this study, B. mori silk fibroin fibers grafted with methacrylonitrile (MAN) were characterized using Raman and IR spectroscopies to gain information on their structural modifications, bioactivity, and metal (Ag) adsorption.
The Raman technique allowed us to evaluate the content of the grafting agent, study the grafting mechanism, which involved Ser and Trp residues, and evaluate the minor structural changes induced by grafting (i.e., the decrease of unordered domains). The IR technique confirmed the previous findings and allowed us to study the effects of grafting on the orientational order/disorder of fibers.
The same techniques were applied to study the grafted samples' metal binding and bioactivity. Silver adsorption was found to be proportional to the MAN content of fibers, caused a shift towards higher wavenumbers of the nitrile group, and induced minor structural changes on silk fibroins, evidenced by the shift and increased FWHM of the Amide I and Amide II bands. The bioactivity test in SBF showed similar structural variations compared to Ag + adsorption, but evidenced a protective role of the grafting agent, which showed only a minimal release (5-7% in weight) after 28 days of immersion in the SBF solution and prevented silk fibroin weight loss. IR spectroscopy allowed the detection of a calcium phosphate phase on modified fibers, which was higher in the samples incorporating higher amounts of the grafting agent.
These preliminary results were encouraging about the positive effect of grafting on the bioactivity and metal adsorption properties of MAN-grafted silk fibroin fibers fostering their possible application as biomedical materials.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules28062551/s1 Table S1: Weight gain % of the silk fibers under study after ageing in the Ag + -containing solution for 48 h; Table S2. Weight gain % of the silk fibers under study after 7 and 28 days in the SBF solution; Figure S1. Raman spectra of B. mori silk fibroin fibers before and after grafting with MAN at different weight gains (i.e., 10%, 25%, 32.5%, 40%, and 60%) in three different spectral ranges: (a) 3350-2180 cm −1 , (b) 1730-1530 cm −1 , and (c) 1500-530 cm −1 . The spectra are normalized to the intensity of the Amide I band. The main bands assignable to β-sheet (β) or unordered (Un) conformation as well as to tyrosine (Y), phenylalanine (F), tryptophan (W), and the grafting agent (MAN) are indicated; Figure S2. IR spectra recorded in the parallel orientation on B. mori silk fibroin fibers before and after grafting with MAN at different weight gains (i.e., 10%, 25%, 32.5%, 40%, and 60%) in three different spectral ranges: (a) 2280-2200 cm −1 , (b) 1720-1485 cm −1 , and (c) 1485-950 cm −1 . The spectra are normalized to the intensity of the Amide I band. The main bands that underwent changes upon MAN grafting are indicated; Figure S3. IR spectra recorded in the perpendicular orientation on B. mori silk fibroin fibers before and after grafting with MAN at different weight gains (i.e., 10%, 25%, 32.5%, 40%, and 60%) in three different spectral ranges: (a) 2280-2200 cm −1 , (b) 1720-1485 cm −1 , and (c) 1485-950 cm −1 . The spectra are normalized to the intensity of the Amide I band. The main bands that underwent changes upon MAN grafting are indicated; Figure S4. Trend of the I 1404 /I Amide I Raman intensity ratio as a function of the weight gain (i.e., MAN content w/w%) for the silk fibroin fibers grafted with MAN; Figure S5. Trend of the I 1555 /I Amide I Raman intensity ratio as a function of the weight gain (i.e., MAN content w/w%) for the silk fibroin fibers grafted with MAN; Figure S6. Trend of the I 854 /I 829 Raman intensity ratio as a function of the weight gain (i.e., MAN content w/w%) for the silk fibroin fibers grafted with MAN; Figure S7. Trend of the A 1230 /A 1260 absorbance ratio as a function of the weight gain (i.e., MAN content w/w%); Figure S8. Values of the Ω Amide III marker as obtained from the IR spectra recorded in the parallel and perpendicular orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN); Figure S9. Values of the A 1217 /A 1230 absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in the Ag + -containing solution for 48 h; Figure S10. Values of the FWHM of Amide I (a) and Amide II (b) as obtained from the IR spectra recorded in the parallel orientation on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in the Ag + -containing solution for 48 h; Figure S11. Values of the FWHM of Amide I (a) and Amide II (b) as obtained from the IR spectra recorded in the perpendicular orientation on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in the Ag + -containing solution for 48 h; Figure S12. Values of the A 1230 /A 1260 absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in the Ag + -containing solution for 48 h; Figure S13. Trend of the % variation of the FWHM of Amide I (in the parallel spectra) and Amide II (in the perpendicular spectra) upon ageing in the Ag + solution for 48 h, as a function of the % weight gain due to silver uptake. Graph (a) reports data from all the samples, while (b) reports trends calculated excluding the 25% MAN-grafted sample (outlier); Figure S14. Values of the A Amide I /A Amide II absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in the Ag + -containing solution for 48 h; Figure S15. (a) Trend of the % variation of the A Amide I /A Amide II and A 1230 /A 1260 absorbance ratios (in the perpendicular spectra) and (b) Ω Amide I/Amide II upon ageing in the Ag + solution for 48 h, as a function of the % weight gain due to silver uptake; Figure S16. Values of Ω Amide I / Amide II as obtained from the IR spectra recorded in the parallel and perpendicular orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in the Ag + -containing solution for 48 h; Figure S17. Trend of the I 2237 /I Amide I Raman intensity ratio before and after 7 and 28 days of immersion in the SBF solution; Figure S18. Trend of the I 703 /I Amide I Raman intensity ratio before and after 7 and 28 days of immersion in the SBF solution; Figure S19. Trend of the I 1266 /I 1229 Raman intensity ratio before and after 7 and 28 days of immersion in the SBF solution; Figure S20. Trend of the I 1555 /I Amide I Raman intensity ratio before and after 7 and 28 days of immersion in the SBF solution; Figure S21. Trend of the I 1404 /I Amide I Raman intensity ratio before and after 7 and 28 days of immersion in the SBF solution; Figure S22. Trend of the I 854 /I 829 Raman intensity ratio before and after 7 and 28 days of immersion in the SBF solution; Figure S23. Trend of the % variation of the A 1060/ A Amide I absorbance ratio ((a) parallel spectra; (b) perpendicular spectra) upon ageing in SBF for 28 days, as a function of the % weight gain; Figure S24. Values of the A 1217 /A 1230 absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in SBF; Figure S25. Values of the FWHM of Amide I (a) and Amide II (b) bands as obtained from the IR spectra recorded in the parallel orientation on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in SBF; Figure S26. Values of the A Amide I /A Amide II absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in SBF; Figure S27. Values of the Ω Amide I / Amide II marker as obtained from the IR spectra recorded in the parallel and perpendicular orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in SBF; Figure S28. Values of the A 1230 /A 1260 absorbance ratio as obtained from the IR spectra recorded in the parallel (a) and perpendicular (b) orientations on the samples under study (control = untreated fibers, and MAN-grafted fibers containing different amounts of MAN) before (t0) and after ageing of the fibers in SBF; Figure S29. Trend of the % variation of the A 1230 /A 1260 absorbance ratio upon ageing in SBF for 28 days as a function of the % weight gain (perpendicular spectra).

Conflicts of Interest:
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
Sample Availability: Samples of the compounds are available from the authors.