Activation of Peracetic Acid by CoFe2O4 for Efficient Degradation of Ofloxacin: Reactive Species and Mechanism

Peroxyacetic acid (PAA)-based advanced oxidation processes (AOPs) have attracted much attention in wastewater treatment by reason of high selectivity, long half-life reactive oxygen species (ROS), and wider applicability. In this study, cobalt ferrite (CoFe2O4) was applied to activate PAA for the removal of ofloxacin (OFX). The degradation of OFX could reach 83.0% via the CoFe2O4/PAA system under neutral conditions. The low concentration of co-existing anions and organic matter displayed negligible influence on OFX removal. The contributions of hydroxyl radicals (·OH), organic radicals (R-O·), and other reactive species to OFX degradation in CoFe2O4/PAA were systematically evaluated. Organic radicals (especially CH3C(O)OO·) and singlet oxygen (1O2) were verified to be the main reactive species leading to OFX destruction. The Co(II)/Co(III) redox cycle occurring on the surface of CoFe2O4 played a significant role in PAA activation. The catalytic performance of CoFe2O4 remained above 80% after five cycles. Furthermore, the ecotoxicity of OFX was reduced after treatment with the CoFe2O4/PAA system. This study will facilitate further research and development of the CoFe2O4/PAA system as a new strategy for wastewater treatment.


Introduction
Fluoroquinolones (FQs) are synthetic medicines with a broad antibacterial spectrum and potent antibacterial activity used in medicine and aquaculture.Only 30-70% of FQs are digested and absorbed by organisms and excreted directly into the ecosystem as natural or metabolites [1][2][3].Currently, FQs have been widely detected in wastewater, surface water, groundwater, and even drinking water with trace concentrations ranging from ng/L to µg/L [4].FQs have attracted widespread public attention as a new environmental pollutant with the environmental risk of induced resistance genes [5].Ofloxacin (OFX) is a typical representative of FQs and is hard to degrade in the natural environment [6].Additionally, OFX could form chelates with some metal ions, resulting in more biotoxicity and resistance to degradation [7].Therefore, it is essential to efficiently remove OFX from the aqueous environment.Advanced oxidation processes (AOPs) are promising methods for antibiotics in water due to the short reaction time and higher efficiency [8][9][10][11].However, the availability of conventional AOPs is dependent on ideal pH and less interfering substance.
Peroxyacetic acid (PAA) has been widely utilized as a disinfectant, bleaching agent, sterilizer, oxidizing agent, and polymerization catalyst in the food processing, medical, chemical, and paper industries [12,13].Compared with conventional disinfectants, PAA shows enhanced bactericidal ability, low pH dependence, flexible operation, and less toxicity via by-products [14].Aside from its disinfectant properties, PAA possesses a high redox potential of 1.96 V, making it capable of degrading organic pollutants [13].PAA can also be activated by UV irradiation, heat, and transition metal activation, producing reactive oxygen species (ROS) with high oxidation ability as a result, such as hydroxyl radicals (•OH) and organic radicals (CH 3 C(O)O•, CH 3 C(O)OO•), due to the fact that it has an easily activated O-O bond that is also contained in hydrogen peroxide (H 2 O 2 ), peroxydisulfate (PDS), and peroxymonosulfate (PMS) [15,16].The slight dependence on pH, anti-interference, and less disinfection byproducts are the more interesting features of PAA-based AOPs over conventional AOPs [17].Moreover, transition metals are considered to be the optimal activation method due to their natural abundance, lack of external energy, and highly catalytic performance [18].
Co(II) is generally considered the most effective activator for PAA among the transition metals (e.g., Co(II), Fe(II), Mn(II), and Cu(II)) [16,19,20].The mechanism of PAA activation by metal ions is shown in Equations ( 1) and ( 2) [21].However, the poor reusability, secondary pollution, and toxicity of metal catalysts are the main hindrances to such homogeneous activation methods [20,[22][23][24].For this, developing heterogeneous cobalt catalysts for PAA activation is essential.Spinel ferrites are widely used as wave-absorbing materials and nanocomposite films due to their excellent stability and fascinating magnetic properties [25][26][27].Additionally, they are also a good choice to serve as the catalytic material in AOPs.Recently, cobalt ferrite (CoFe 2 O 4 ) has been demonstrated as an excellent PAA activator, owing to its strong structural stability, low metal ion leaching, bimetallic components, and magnetic properties [28][29][30][31][32].As such, the application of CoFe 2 O 4 in PAA activation might hold promise for the efficient degradation of FQs in wastewater.Even though the CoFe 2 O 4 /PAA system has been studied for removing pollutants from water, there is limited information on what active species are present in the system and how CoFe 2 O 4 activates PAA to specifically degrade contaminants.
This study aimed to assess the roles of reactive species of CoFe 2 O 4 /PAA in degrading OFX and further elucidate its catalytic mechanism.Firstly, the degradation efficiency of OFX in the CoFe 2 O 4 /PAA system was explored according to the effects of CoFe 2 O 4 dosages, PAA concentration, initial pH, and water matrix (common anions and HA).Subsequently, the stability and reusability of CoFe 2 O 4 on PAA activation were evaluated.Furthermore, the dominant reactive species in the CoFe 2 O 4 /PAA system for OFX removal was identified.Finally, the degradation pathway of OFX and the toxicity of OFX before and after its treatment in the CoFe 2 O 4 /PAA system were proposed.

Characterization of CoFe 2 O 4
Figure 1a shows the XRD spectra of CoFe 2 O 4 before and after the reaction.There are seven well-defined peaks in the XRD spectra, which confirm the crystal structure and excellent crystallinity of CoFe 2 O 4 , and the diffraction peaks correspond to the characteristic peaks in the standard spectrum of CoFe 2 O 4 .Moreover, the positions of the characteristic diffraction peaks of CoFe 2 O 4 did not change before and after the reaction, indicating the great structural stability of CoFe 2 O 4 .The FT-IR spectra of CoFe 2 O 4 before and after the reaction are presented in Figure 1b.The absorption peaks nearing 3421 cm −1 and 1625 cm −1 correspond to the stretching and bending vibration of hydroxyl groups, respectively, which are mainly from surface-adsorbed water molecules [33].Additionally, the peak nearing 580 cm −1 can be interpreted as a stretching vibration of metal-oxygen (M-O) and it could further verify the formation of Co/Fe-O [34].Additionally, it is obvious that CoFe 2 O 4 exhibits irregular particles with a size of about 100 nm (Figure 1c), and the elemental mapping image (Figure 1d) revealed that Co, Fe, and O were uniformly distributed on the surface of CoFe 2 O 4 .

Degradation of OFX by the System of CoFe2O4/PAA
The degradation of OFX under different systems is shown in Figure 2a.OFX w almost not degraded in the system of H2O2 and PAA alone, indicating that it was negli ble to oxidize OFX with H2O2 and PAA.Only 5.5% and 6.0% of OFX were removed CoFe2O4 alone and the CoFe2O4/H2O2 system, respectively, suggesting that CoFe2O4 h weak physical adsorption capability to OFX and trouble activating H2O2.Compared w other systems, CoFe2O4 was able to degrade OFX effectively, and the removal rate reach 83.0% after 45 min, which implies that CoFe2O4 is an effective catalyst for PAA activatio This is due to the activation of PAA adsorption on the surface of CoFe2O4 and its deco position to produce highly reactive radicals, which leads to the degradation of orga pollutants [17].Compared with other systems, CoFe 2 O 4 was able to degrade OFX effectively, and the removal rate reached 83.0% after 45 min, which implies that CoFe 2 O 4 is an effective catalyst for PAA activation.This is due to the activation of PAA adsorption on the surface of CoFe 2 O 4 and its decomposition to produce highly reactive radicals, which leads to the degradation of organic pollutants [17].
In addition, the decomposition of PAA during the reaction is displayed in Figure S1.Almost all of the PAA decomposed after 45 min.It is noteworthy that the catalytic decomposition of PAA can be accompanied by the production of a large number of fine carbon sources, including methanol (CH 3 OH), acetic acid (CH 3 COOH), and formaldehyde (HCHO) (Equations ( 3)-( 6)) [16,17], and the PAA solution itself contains a certain amount of acetic acid, which can be used in the biological treatment process.Therefore, the CoFe 2 O 4 /PAA process in combination with biological treatment processes has great potential for application in the field of water treatment.In addition, the decomposition of PAA during the reaction is displayed in Figure S Almost all of the PAA decomposed after 45 min.It is noteworthy that the catalytic decom position of PAA can be accompanied by the production of a large number of fine carbo sources, including methanol (CH3OH), acetic acid (CH3COOH), and formaldehyd (HCHO) (Equations ( 3)-( 6)) [16,17], and the PAA solution itself contains a certain amou of acetic acid, which can be used in the biological treatment process.Therefore, th

Influence of Reaction Factors
The effect of CoFe 2 O 4 dosage on OFX degradation is shown in Figure 2b.The removal rates of OFX were 76.2%, 80.4%, 81.9%, and 79.1% when the CoFe 2 O 4 dose was 0.05, 0.10, 0.20, and 0.40 g/L, respectively.At the same time, the corresponding k obs increased from 0.039 to 0.073 min −1 .An increase in surface active sites with increasing CoFe 2 O 4 accelerates the decomposition of PAA and promotes the generation of ROS.However, CoFe 2 O 4 itself scavenges ROS when added in excess [35] and causes agglomeration and precipitation, which reduces active sites [21].
The effect of PAA concentration on OFX degradation is shown in Figure 2c.The removal rate of OFX increased from 44.6% to 85.9% when the PAA concentration was raised from 0.1 mM to 0.8 mM, and the k obs also grew by 3.2 times.The increase in PAA concentration can fully utilize the active sites of CoFe 2 O 4 to produce more ROS.Nevertheless, the formation of ROS is relatively slowed when active sites are saturated with PAA because of the high PAA concentration.Furthermore, too much PAA has a quenching impact on ROS [21,36].
The pH is a critical factor affecting OFX removal in the CoFe 2 O 4 /PAA system.The effects of different initial pH on OFX degradation are shown in Figure 2d.In neutral conditions, OFX degraded significantly better than in acidic or alkaline conditions.pH determines the morphology of PAA in aqueous solutions.Since PAA has a pK a of 8.2, it mostly resides in its neutral form (PAA 0 ) when the pH is below 8.2, and in its deprotonated form (PAA − ) when the pH is above 8.2 [15].In addition, pH also affects the surface charge of CoFe 2 O 4 whose isoelectric point is 6.5 (Figure S2).Therefore, PAA in solution mainly exists in the form of PAA − under alkaline conditions, while the surface of CoFe 2 O 4 is negatively charged and electrically repulsive, which reduces the chance of contact between PAA and CoFe 2 O 4 .Acidic conditions cause the surface of CoFe 2 O 4 to become positively charged, and the O-O bond of PAA can easily form a hydrogen bond with H + to become positively charged [37].

Influence of Water Matrix
The widespread presence of anions and dissolved organic matter (DOM) in the aqueous environment could affect OFX degradation by scavenging ROS and forming complexes with metal ions.As shown in Figure 2e, Cl − and SO 4 2− have a slight effect on OFX degradation in the CoFe 2 O 4 /PAA system.As SO 4 2− typically does not react with ROS (•OH, R-O•), its presence in high quantities has no significant impact on OFX removal.According to previous studies, Cl − generally plays two roles in the PAA system: (i) Cl − can react directly with PAA to produce the secondary oxidant HOCl (Equation ( 7)) [38], and (ii) Cl − also reacts with ROS to form chlorine-containing reactive species (Cl•, HOCl• − , Cl 2 •− ) (Equations ( 8)-( 11)), which have different sensitivities to different pollutants [39].
High concentrations of NO 3 − significantly inhibited the degradation of OFX.It is possible that NO 3 − reacts with reactive radicals to generate NO 2 − , which also competes with OFX for ROS [40].Obviously, HCO 3 − strongly suppressed the degradation of OFX.The removal rates of OFX were only 69.3%, 15.7%, 10.5%, and 9.0% when the concentration of HCO 3 − in the system was 1, 5, 10, and 20 mM, respectively.HCO 3 − is a common scavenger of •OH and hardly reacts with R-O•, therefore the inhibitory effect on OFX degradation is not a competition for reactive radicals [16].As previously reported, the CoFe 2 O 4 surface forms a Co-HCO 3 − complex with HCO 3 − , which blocks PAA activation [16,41].Moreover, HCO 3 − is a buffer substance that affects the pH in the reaction system [18], and the weak alkaline conditions are not favorable for OFX degradation by CoFe 2 O 4 /PAA.HA plays a part in inhibiting the removal of OFX in the CoFe 2 O 4 /PAA system, especially at high concentrations of HA.HA is a common radical scavenger [39] and can readily adsorb onto the surface of CoFe 2 O 4 to form unreactive complexes [30,42].

Reusability and Stability of the CoFe 2 O 4
The reusability of CoFe 2 O 4 was evaluated with cycling experiments, which were carried out under the same reaction system.As can be seen from Figure 3a, the removal efficiency of OFX could still be kept over 80% after five cycles, which indicates that CoFe 2 O 4 has a stable catalytic performance for PAA.Additionally, the highest leaching amount of cobalt ions was only 0.055 mg/L in cycling experiments (Figure 3b), while iron ions were not detected.Moreover, the XRD and FT-IR analyses of CoFe 2 O 4 before and after the reaction also further confirmed the structural stability of CoFe

Applicability of CoFe2O4/PAA System
The degradation effect of the CoFe2O4/PAA system on other three different FQs w investigated and the results are shown in Figure 3c.Norfloxacin (NOR), Ciprofloxac (CIP), and Enrofloxacin (ENR) were degraded to a great extent after 45 min and the moval rates could reach 77.0%, 75.5%, and 81.5%, respectively, suggesting that t CoFe2O4/PAA system has the same great degradation performance as other FQs.In ad tion, the performance of the CoFe2O4/PAA process for OFX degradation was evaluated both tap water and surface water.It was pleasantly surprising that the removal efficien of OFX in tap water and surface water was decreased by only 1.4% and 2.5%, respective (Figure 3d).The information about these two water types is listed in Table S2.The reas for this slight inhibition may be that coexisting ions and organic matter in actual wa can consume ROS.Thereby, the CoFe2O4/PAA process has promising potential for prac cal application.

Identification and Analysis of Reactive Species
It has been reported that various reactive species might be involved in PAA-bas AOPs, such as •OH, O2 ) and high-valent metal sp cies (Co (IV), Fe (IV)) [16,43].Therefore, a quenching experiment was conducted to ide tify ROS produced in the CoFe2O4/PAA system.Tertiary butyl alcohol (TBA) is a typi

Applicability of CoFe 2 O 4 /PAA System
The degradation effect of the CoFe 2 O 4 /PAA system on other three different FQs was investigated and the results are shown in Figure 3c.Norfloxacin (NOR), Ciprofloxacin (CIP), and Enrofloxacin (ENR) were degraded to a great extent after 45 min and the removal rates could reach 77.0%, 75.5%, and 81.5%, respectively, suggesting that the CoFe 2 O 4 /PAA system has the same great degradation performance as other FQs.In addition, the performance of the CoFe 2 O 4 /PAA process for OFX degradation was evaluated in both tap water and surface water.It was pleasantly surprising that the removal efficiency of OFX in tap water and surface water was decreased by only 1.4% and 2.5%, respectively (Figure 3d).The information about these two water types is listed in Table S2.The reason for this slight inhibition may be that coexisting ions and organic matter in actual water can consume ROS.Thereby, the CoFe 2 O 4 /PAA process has promising potential for practical application.

Identification and Analysis of Reactive Species
It has been reported that various reactive species might be involved in PAA-based AOPs, such as ) and high-valent metal species (Co (IV), Fe (IV)) [16,43].Therefore, a quenching experiment was conducted to identify ROS produced in the CoFe  CHCl3 can act as a scavenger of O2 •− , which rapidly reacts with O2 [44].The degradation of OFX was not affected by CHCl3, which means O2 •− h little involvement.Additionally, FFA is widely used as a quencher of 1 O2 (k FFA/1O2 = 1.2 ×1 M −1 s −1 ) [16].According to the results in Figure 4a, the inhibitory effect of FFA on OFX de radation is significantly higher than that of other quenchers, indicating that 1 O2 plays important role in the degradation process.Meanwhile, the addition of excess quenche can consume a part of PAA (Figures 4b and S4).
The formation of high-valent metal species like Co(IV) and Fe(IV) in the system cou be probed by methyl phenyl sulfoxide (PMSO).PMSO was reported to be converted methyl phenyl sulfone (PMSO2) with high-valent metal species via an oxygen atom tran fer route [35,46], in contrast to free radicals, which form hydroxylated products [47]  •− had little involvement.Additionally, FFA is widely used as a quencher of 1 O 2 (k FFA/1O2 = 1.2 × 10 8 M −1 s −1 ) [16].According to the results in Figure 4a, the inhibitory effect of FFA on OFX degradation is significantly higher than that of other quenchers, indicating that 1 O 2 plays an important role in the degradation process.Meanwhile, the addition of excess quenchers can consume a part of PAA (Figures 4b and S4).
The formation of high-valent metal species like Co(IV) and Fe(IV) in the system could be probed by methyl phenyl sulfoxide (PMSO).PMSO was reported to be converted to methyl phenyl sulfone (PMSO 2 ) with high-valent metal species via an oxygen atom transfer route [35,46], in contrast to free radicals, which form hydroxylated products [47].It turned out that PMSO 2 occurred in the CoFe 2 O 4 /PAA system, but the conversion rate of PMSO to PMSO 2 was only 8.0% (Figure S5).Moreover, the removal rate of OFX was only decreased by 2.5% when excess PMSO was introduced (Figure S6).The results demonstrated that high-valent metal species (Co(IV), Fe(IV)) serve a minor role in OFX degradation.
EPR tests were performed to further verify the generation of ROS in the CoFe 2 O 4 /PAA system.DMPO is the spin-trapping agent of •OH and O 2 •− , while DIPPMPO and TEMP serve as the trapping agents for R-O• and 1 O 2 , respectively [45,48] .As shown in Figure 4c,d 12)) and is less reactive toward most organic compounds [20,49].In contrast, CH 3 C(O)OO• is capable of strong oxidation [19].Thus, CH 3 C(O)OO• is the main R-O• involved in OFX degradation.Based on the above analysis, it can be inferred that R-O• (especially CH 3 C(O)OO•) and 1 O 2 are the major reactive species responsible for OFX removal in the CoFe 2 O 4 /PAA system.

Activation Mechanism
To further illustrate the activation mechanism of CoFe 2 O 4 on PAA, XPS analysis of CoFe 2 O 4 before and after the reaction was performed.The XPS full spectrum scans of CoFe 2 O 4 before and after the reaction are shown in Figure 5a, which reveals the presence of Co, Fe, and O. Figure 5b displays the XPS peak-fitting spectra of Co 2p before and after the reaction of CoFe 2 O 4. The Co(III) exhibits distinctive peak positions at 778.9 eV (Co 2p 3/2 ) and 794.1 eV (Co 2p 1/2 ), whereas Co(II) is associated with peak locations at 780.6 eV (Co 2p 3/2 ) and 795.4 eV (Co 2p 1/2 ).The proportion of Co(III) declined from 53.42% to 49.56%, whereas the Co(II) increased from 46.58% to 50.44%.These observations indicate the presence of a redox cycle involving ≡Co(II)/≡Co(III) on the surface of CoFe 2 O 4 throughout the reaction [18,45].
Figure 5c shows the XPS peak-fitting spectra of Fe 2p before and after the CoFe 2 O 4 reaction.The peaks of Fe(II) at Fe 2p 3/2 and Fe 2p 1/2 correspond to binding energies of 710.0 eV and 723.4 eV, respectively, while those for Fe(III) are 711.8eV and 725.1 eV.The proportion of Fe(II) declined from 58.68% to 55.88% and the corresponding increase in Fe(III) from 41.32% to 44.12% suggests the presence of a redox cycle with ≡Fe(III)/≡Fe(II) on the CoFe 2 O 4 .Nevertheless, the involvement of ≡Fe(III)/≡Fe(II) in the activation of PAA is minimal in comparison to ≡Co(II)/≡Co(III) [42].It should be noticed that the presence of Fe in CoFe 2 O 4 promotes the transformation of Co(III) to Co(II) (Equation ( 13)), which improves the electron-transfer capacity of the catalyst [45].Figure 5c shows the XPS peak-fitting spectra of Fe 2p before and after the CoFe2O reaction.The peaks of Fe(II) at Fe 2p3/2 and Fe 2p1/2 correspond to binding energies of 710.0 eV and 723.4 eV, respectively, while those for Fe(III) are 711.8eV and 725.1 eV.The pro portion of Fe(II) declined from 58.68% to 55.88% and the corresponding increase in Fe(III from 41.32% to 44.12% suggests the presence of a redox cycle with ≡Fe(III)/≡Fe(II) on the CoFe2O4.Nevertheless, the involvement of ≡Fe(III)/≡Fe(II) in the activation of PAA is min imal in comparison to ≡Co(II)/≡Co(III) [42].It should be noticed that the presence of Fe in CoFe2O4 promotes the transformation of Co(III) to Co(II) (Equation ( 13)), which improve the electron-transfer capacity of the catalyst [45].
Fe II + Co III → Co II + Fe III (13 As shown in Figure 5d, the peaks of lattice oxygen, surface hydroxyl oxygen, and adsorbed oxygen are located at 529.7 eV, 531.5 eV, and 533.2 eV, respectively.The lattice oxygen decreased from 75.75% to 70.83%, while surface hydroxyl oxygen increased from 19.50% to 21.39%, and adsorbed oxygen rose from 4.75% to 7.78%.The decrease in lattice oxygen can be attributed to the reduction of Co(III) to Co(II), while the increase in surface hydroxyl oxygen can be explained by the formation of Co-OH and Fe-OH (Equations ( 14)-( 16)) [30,42].As shown in Figure 5d, the peaks of lattice oxygen, surface hydroxyl oxygen, and adsorbed oxygen are located at 529.7 eV, 531.5 eV, and 533.2 eV, respectively.The lattice oxygen decreased from 75.75% to 70.83%, while surface hydroxyl oxygen increased from 19.50% to 21.39%, and adsorbed oxygen rose from 4.75% to 7.78%.The decrease in lattice oxygen can be attributed to the reduction of Co(III) to Co(II), while the increase in surface hydroxyl oxygen can be explained by the formation of Co-OH and Fe-OH (Equations ( 14)-( 16)) [30,42].
Therefore, the degradation of OFX in the CoFe  19) and ( 20)), which has a weak oxidizing capacity and is prone to forming O 2 •− by deprotonation (Equation ( 21)).Moreover, 1 O 2 could be obtained from the recombination of O 2 •− (Equation ( 22)).Although the role of Fe in the activation of PAA might be disregarded, the interaction of Fe with Co speeds up the rate of electron transfer at the catalytic interface as well as the adsorption of PAA [42,45].

Degradation Pathway and Toxicity Assessment
Three-dimensional fluorescence spectroscopy (3D-EEM) was used to observe the compositional changes of organic matter during OFX degradation and to perform a preliminary analysis of the evolution of the molecular structure.As shown in Figure 6a, OFX showed double fluorescence peaks.The two peaks were located in the ranges of E X /E m = (275-300 nm)/(400-575 nm) and E X /E m = (300-375 nm)/(400-575 nm), which belong to humic acid-like substances.Meanwhile, it further reflects the aromatic structure of OFX and functional groups such as carboxyl groups (-COOH) and carbonyl groups (-C=O) [50].As the reaction went on, the intensity of the two fluorescence peaks gradually weakened and the center of the peaks was slightly shifted to the left (Figure 6a-d), implying that the conjugated structure of OFX was continuously destroyed to form intermediate products and that the CoFe 2 O 4 /PAA system could degrade OFX effectively.The degradation intermediates of OFX in the CoFe2O4/PAA system were detected LC-TOF-MS (Figure S7) and summarized in Table S4.Four possible degradation pathw of OFX were proposed based on experimental results and related literature (Figure 7  The degradation intermediates of OFX in the CoFe 2 O 4 /PAA system were detected by LC-TOF-MS (Figure S7) and summarized in Table S4.Four possible degradation pathways of OFX were proposed based on experimental results and related literature (Figure 7).In pathway I, opening of the oxazine ring and hydroxylation of the quinolone moiety in OFX occurred to form P1 (m/z = 354), followed by the generation of P2 (m/z = 314) via the cleavage of C=C and C-N bonds, and was further demethylated to produce P3 (m/z = 283) [51].In pathway II, decarboxylation and hydroxylation of the quinolone moiety in OFX formed P4 (m/z = 334), and P4 was oxidized to produce P5 (m/z = 205), which was transformed to P6 (m/z = 194) via the cleavage of the C=C bond and hydroxylation [52,53].In pathway III, P7 (m/z = 280) was obtained by the epoxidation and hydroxylation of piperazine from OFX and was then further converted to P8 (m/z = 224) via decarboxylation, deamination, and demethylation.As part of pathway IV, OFX was first defluorinated to form P9 (m/z = 344), followed by the generation of P10 (m/z = 327) and P11 (m/z = 300) via demethylation and decarboxylation, respectively, and P12 (m/z = 149) was obtained by the ring-opening of P11 [51,52].Eventually, these intermediates are mineralized into inorganic molecules such as CO 2 , H 2 O, NO 3 − , and F − .As a whole, ring opening, decarboxylation, defluorination, hydroxylation, demethylation, and bond cleavage contribute to OFX degradation.
Molecules 2023, 28, x FOR PEER REVIEW 13 of 18 To evaluate the toxicity variation, toxicity software (T.E.S.T., Version 5.1.2) based on quantitative structure-activity relationship (QSAR) was applied to predict the ecotoxicity of OFX and its detected intermediates, which include acute toxicity, bioconcentration factors, developmental toxicity, and mutagenicity.It is worth mentioning that OFX exhibited toxicity, as seen by its 96 h Fathead minnow LC50 value of 1.24 mg/L (Figure 8a).This value was lower than that of the intermediates, suggesting a significant reduction in acute toxicity following degradation.As displayed in Figure 8b, the obtained bioaccumulation factor of OFX and its intermediates indicated that all intermediates except P1, P2, P3, P7, and P10 were lower than OFX.Additionally, the developmental toxicity value of OFX was higher than most of the intermediates (Figure 8c), proving a decrease in developmental toxicity after degradation except for P4 and P9, and P12 even showed developmental nontoxicants.OFX and most of the intermediates were classified as mutagenicity-positive, and P11 was even considered mutagenicity-negative (Figure 8d).After the reaction, it was found that the total toxicity of OFX was lower than before the reaction, indicating that the CoFe2O4/PAA system offered a high-level potential for toxicity reduction.To evaluate the toxicity variation, toxicity software (T.E.S.T., Version 5.1.2) based on quantitative structure-activity relationship (QSAR) was applied to predict the ecotoxicity of OFX and its detected intermediates, which include acute toxicity, bioconcentration factors, developmental toxicity, and mutagenicity.It is worth mentioning that OFX exhibited toxicity, as seen by its 96 h Fathead minnow LC 50 value of 1.24 mg/L (Figure 8a).This value was lower than that of the intermediates, suggesting a significant reduction in acute toxicity following degradation.As displayed in Figure 8b, the obtained bioaccumulation factor of OFX and its intermediates indicated that all intermediates except P1, P2, P3, P7, and P10 were lower than OFX.Additionally, the developmental toxicity value of OFX was higher than most of the intermediates (Figure 8c), proving a decrease in developmental toxicity after degradation except for P4 and P9, and P12 even showed developmental non-toxicants.OFX and most of the intermediates were classified as mutagenicity-positive, and P11 was even considered mutagenicity-negative (Figure 8d).After the reaction, it was found that the total toxicity of OFX was lower than before the reaction, indicating that the CoFe 2 O 4 /PAA system offered a high-level potential for toxicity reduction.

Degradation Experiments
Degradation experiments were conducted in a 250 mL glass reactor with shock s ring (Thermostatic shaker SYC-2A, Shanghai Bunting Instrument Co., Shanghai, Chi at a speed of 150 rpm.Firstly, 100 mL of 20 µM OFX solution was transferred to the react followed by the addition of a predetermined concentration of PAA, and then the solutio pH was adjusted with diluted H2SO4 and NaOH.The experiments were initiated with addition of CoFe2O4.All the experiments were maintained at 23 ± 2 °C.Samples w   S5.All chemicals were of analytical grade and used without further purification.

Degradation Experiments
Degradation experiments were conducted in a 250 mL glass reactor with shock stirring (Thermostatic shaker SYC-2A, Shanghai Bunting Instrument Co., Shanghai, China) at a speed of 150 rpm.Firstly, 100 mL of 20 µM OFX solution was transferred to the reactor, followed by the addition of a predetermined concentration of PAA, and then the solution's pH was adjusted with diluted H 2 SO 4 and NaOH.The experiments were initiated with the addition of CoFe 2 O 4 .All the experiments were maintained at 23 ± 2 • C. Samples were collected within a predetermined time (0, 2, 5, 10, 20, 30, 45 min), and quenched by Na 2 S 2 O 3 , then filtered with a 0.22 µm aqueous filter membrane for analysis.
Different concentrations of anions (Cl − , SO 4 2− , NO 3 − , HCO 3 − ) and HA were added to the reaction solution to investigate the effect of water matrices on OFX degradation.ROS in the system were identified by radical scavengers (TBA, MeOH, CHCl 3 , FFA) and verified by probe compounds (pCBA, NAP, PMSO).NOR, CIP, and ENR were used to evaluate the applicability of CoFe 2 O 4 /PAA systems for FQs.In order to evaluate the stability of the catalytic performance of CoFe 2 O 4 , the used catalyst was desorbed by alkali and then washed with deionized water to neutral and dried at 60 • C. All experiments were conducted at least twice, and the error bar shown in the figure represents the standard deviation between replicates.
The stock solution of OFX (30 mg/L) was obtained by dissolving OFX in pure water with stirring and was stored at 4 • C. The working solution of OFX was diluted from the stock solution.Kinetic analysis of OFX degradation was depicted in Text S1.

Analytical Methods
The surface morphology and chemical composition of CoFe 2 O 4 were characterized using scanning microscopy (SEM, Scios 2 HiVac, FEI, Danville, CA, USA) equipped with energy-dispersive X-ray spectroscopy (EDS).The crystal phase structure of CoFe 2 O 4 before and after the reaction was determined through an X-ray diffractometer (XRD, Smart-Lab SE, Rigaku, Japan) with Cu-Ka radiation over the range of 10 • to 80 • , and Fourier transform infrared spectroscopy (FT-IR, Nicolet iS20, Thermo Scientific, Waltham, MA, USA) was used to determine functional groups.The surface elemental composition of CoFe 2 O 4 before and after the reaction were analyzed by X-ray photoelectron spectroscopy (XPS, K-Alpha, Thermo Scientific, USA), and the zeta potential of CoFe 2 O 4 was measured using a Zeta potentiometer (Nano ZS90, Malvern, UK).
The PAA stock solution was calibrated weekly by titration, and the concentrations of peroxide and hydrogen peroxide in the PAA stock solution were determined using iodimetry and potassium permanganate titration, respectively, so as to calculate the concentration of PAA [39].The residual PAA concentration was determined by N, N-diethyl-pphenylenediamine (DPD) spectrophotometry [15].The concentration of OFX, NOR, CIP, ENR, pCBA, NAP, PMSO, and PMSO 2 were detected by high-performance liquid chromatography (HPLC, Agilent 1260, Santa Clara, CA, USA) coupled with an Agilent EC-C18 column and the details of the conditions are presented in Table S6.The concentrations of cobalt and iron ions were analyzed using inductively coupled plasma-atomic emission spectrometry (ICP-AES, ICP-5000, Focused Photonics Inc, Shanghai, China), and pH values were measured by a pH meter (pH-FE28, METTLER TOLEDO, Greifensee, Switzerland).Electron paramagnetic resonance (EPR) tests were carried out to verify the generated ROS using 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 5-Diisopropoxyphosphoryl-5-methyl-1pyrroline N-oxide (DIPPMPO), and 2,2,6,6-tetramethyl-4-piperidinyl (TEMP) as trapping agents.The detection of three-dimensional fluorescence spectra (3D-EEM) was performed on a fluorescence spectrophotometer (Lengguang F98, Shanghai, China).The intermediates of OFX degradation were determined by LC-QTOF-MS (Agilent 1290-6550, USA) coupled with electrospray ionization (ESI).Meanwhile, the biological toxicity of OFX and its oxidation intermediates was evaluated by the Toxicity Estimation Software Tool (T.E.S.T., Version 5.1.2),which is based on the quantitative structure-activity relationship (QSAR) method.

Conclusions
In this study, CoFe 2 O 4 displayed remarkable catalytic performance in the activation of PAA for OFX degradation, and a removal efficiency of 83.0%OFX was achieved within 45 min under neutral conditions.The CoFe 2 O 4/ PAA system exhibited better resistance to anions and HA at low concentrations in water, but NO

Figure 1 .
Figure 1.XRD spectra (a) and FT-IR spectra (b) of CoFe2O4 before and after the reaction.SEM ima of CoFe2O4 (c) with the elemental mapping images of CoFe2O4 (d).

Figure 1 .
Figure 1.XRD spectra (a) and FT-IR spectra (b) of CoFe 2 O 4 before and after the reaction.SEM image of CoFe 2 O 4 (c) with the elemental mapping images of CoFe 2 O 4 (d).

2. 2 .
Performances of OFX Degradation by CoFe 2 O 4 -Activated PAA Oxidation 2.2.1.Degradation of OFX by the System of CoFe 2 O 4 /PAA The degradation of OFX under different systems is shown in Figure 2a.OFX was almost not degraded in the system of H 2 O 2 and PAA alone, indicating that it was negligible to oxidize OFX with H 2 O 2 and PAA.Only 5.5% and 6.0% of OFX were removed by CoFe 2 O 4 alone and the CoFe 2 O 4 /H 2 O 2 system, respectively, suggesting that CoFe 2 O 4 has weak physical adsorption capability to OFX and trouble activating H 2 O 2 .
2 O 4 /PAA system.Tertiary butyl alcohol (TBA) is a typical •OH scavenger at a high reaction rate with •OH (k TBA/•OH = 6.0 × 10 8 M −1 s −1 )[44].As shown in Figure4a, the addition of excess TBA slightly inhibited the removal of OFX, indicating the minor role of •OH in the system.MeOH serves as a common scavenger for both •OH (k MeOH/•OH = 9.7 × 10 8 M −1 s −1 ) and R-O• in the PAA system[32].Hence, the contributions of •OH and R-O• to the degradation of OFX could be distinguished by the use of TBA and MeOH.Obviously, too much MeOH could greatly reduce the removal rate of OFX, which fell from 83.1% to 40.3% (Figure4a).Thus, the inhibition induced by MeOH was attributed to the scavenging effect of R-O• rather than •OH.To further verify this, pCBA (k pCBA/•OH = 5.0 × 10 9 M −1 s −1 ) and NAP (k NAP/R-O• = 9.0 × 10 9 M −1 s −1 ) were utilized as specific probes for •OH, R-O• to explore the contribution to OFX degradation[44,45].As presented in FigureS3, complete removal of NAP occurred within 10 min, whereas only 10% of pCBA was degraded after 45 min.This observation evidenced that a large number of R-O• and less •OH exist in the CoFe 2 O 4 /PAA system.Molecules 2023, 28, x FOR PEER REVIEW 8 of (k MeOH/•OH = 9.7 × 10 8 M −1 s −1 ) and R-O• in the PAA system [32].Hence, the contributions •OH and R-O• to the degradation of OFX could be distinguished by the use of TBA a MeOH.Obviously, too much MeOH could greatly reduce the removal rate of OFX, whi fell from 83.1% to 40.3% (Figure 4a).Thus, the inhibition induced by MeOH was attribut to the scavenging effect of R-O• rather than •OH.To further verify this, pCBA (k pCBA/•OH 5.0 × 10 9 M −1 s −1 ) and NAP (k NAP/R-O•= 9.0 × 10 9 M −1 s −1 ) were utilized as specific probes •OH, R-O• to explore the contribution to OFX degradation [44,45].As presented in Figu S3, complete removal of NAP occurred within 10 min, whereas only 10% of pCBA w degraded after 45 min.This observation evidenced that a large number of R-O• and le •OH exist in the CoFe2O4/PAA system.

2 O 4 /
PAA system was attributed to the generation of reactive species, especially R-O• and 1 O 2 , and Co played an important role in PAA activation.Based on the above discussion, the activation mechanism of CoFe 2 O 4 on PAA was proposed.Initially, the surface ≡Co(II) of CoFe 2 O 4 donates an electron to PAA, which results in the formation of CH 3 C(O)O• and the conversion of ≡Co(II) to ≡Co(III).Subsequently, the generated ≡Co(III) would receive an electron from PAA and recover to ≡Co(II), accompanied by the formation of CH 3 C(O)OO•.Therefore, the ≡Co(II)/≡Co(III) redox cycle is repeated on the catalyst surface to generate R-O• for the degradation of OFX (Equations (17) and (18)).Additionally, the coexisting H 2 O 2 reacts with R-O• (CH 3 C(O)O•, CH 3 C(O)OO•) to produce HO 2 • (Equations (
) pathway I, opening of the oxazine ring and hydroxylation of the quinolone moiety in O occurred to form P1 (m/z = 354), followed by the generation of P2 (m/z = 314) via the cle age of C=C and C-N bonds, and was further demethylated to produce P3 (m/z = 283) [

Figure 7 .
Figure 7.The possible degradation pathway of OFX in CoFe2O4/PAA system.

Figure 7 .
Figure 7.The possible degradation pathway of OFX in CoFe 2 O 4 /PAA system.

3 − , HCO 3 −
, and HA could inhibit OFX degradation at high concentrations.Additionally, CoFe 2 O 4 showed excellent catalytic performance in cycling experiments and great potential for practical wastewater treatment.R-O• (CH 3 C(O)OO•) and 1 O 2 played a dominant role in the degradation of OFX, and the ≡Co(II)/≡Co(III) redox cycle occurring on the surface of CoFe 2 O 4 during the reaction promoted the decomposition of PAA to generate ROS.Finally, the possible degradation pathways of OFX involved ring opening, bond cleavage, decarboxylation, defluorination,