Cytotoxic Natural Products Isolated from Cryptogramma crispa (L.) R. Br.

Parsley fern, Cryptogramma crispa, is a common fern in arctic–alpine regions, and even though this species has been known since ancient times and has been presumed to cause the poisoning of horses, its natural products have not previously been investigated. Here, we characterise 15 natural products isolated from the aerial parts of Cryptogramma crispa, including the previously undescribed compound 3-malonyl pteroside D. The structure determinations were based on several advanced 1D and 2D NMR spectroscopic techniques, Circular Dichroism spectroscopy and high-resolution mass spectrometry. The pteroside derivatives exhibited selective moderate cytotoxic activity against the acute myeloid leukaemia MOLM13 cell line and no cytotoxicity against the normal heart and kidney cell lines, suggesting that their potential anticancer effect should be further investigated.


Introduction
The leptosporangiate fern genus Cryptogramma of the family Pteridaceae comprise nine species and is referred to as parsley fern, as its foliage resembles that of parsley [1].Cryptogramma crispa (L.) R. Br. (Figure 1) is a small rupestral fern widely distributed from the montane to the subalpine zones of the temperate and boreal regions of Europe, with two main distribution centres: the northern area, including the highest mountains in the British Isles and west Scandinavia, and the southern area comprising the highest mountains in south Europe.Within its northern distribution area, C. crispa occurs over a broad altitudinal range, between 20 m and 2800 m above sea level [2,3].
C. crispa is a strongly calcifuge species that only grows on non-carbonaceous bedrocks, often forming patches.This species is quite hardy and can be covered by snow during the winter and receive lots of solar radiation during the summer [3,4].
According to observations within Norwegian traditional medicine, horses that excessively consume parsley ferns are susceptible to colic, thus explaining its Norwegian name hestespreng (which means "horse bloating").Even though the traditional observation has not been unambiguously confirmed in modern studies [5], this species appears to be a potentially interesting source of bioactive natural products.While the existing literature regarding C. crispa is mainly limited to studies of the botanical characteristics of the plant, only a restricted number of studies have explored its phytochemical composition.
In the current literature, only relatively volatile compounds, including alkyl esters, fatty acids, primary alcohols, aldehydes, and alkanes, have hitherto been identified by GCMS from C. crispa [6].Some carotenoid derivatives and shikimic acid derivatives have also been suggested to be present in this plant [7].However, the structures of these compounds remain elusive.To the best of our knowledge, no studies exist in the current literature about the isolation and identification of pure compounds from this plant species.compounds remain elusive.To the best of our knowledge, no studies exist in the current literature about the isolation and identification of pure compounds from this plant species.As part of our ongoing research on the description of new natural products with potential applications as lead compounds of future anticancer drugs, we report on the characterisation of 15 natural compounds from the aerial parts of C. crispa, which have been identified from this plant species for the first time, including a novel compound, as well as their cytotoxic activity towards leukaemia cells and normal kidney and heart cell lines.

Results and Discussion
The methanolic extract obtained from the aerial parts from Cryptogramma crispa was concentrated under reduced pressure and subjected to separation via liquid/liquid partition with petroleum ether followed by ethyl acetate.The components of the aqueous and ethyl acetate phases were further separated via adsorption chromatography, gel filtration chromatography, and preparative HPLC.

Results and Discussion
The methanolic extract obtained from the aerial parts from Cryptogramma crispa was concentrated under reduced pressure and subjected to separation via liquid/liquid partition with petroleum ether followed by ethyl acetate.The components of the aqueous and ethyl acetate phases were further separated via adsorption chromatography, gel filtration chromatography, and preparative HPLC.
Pterosins are sesquiterpenoids with a 1-indanone core structure; the glucoside version is called a pteroside.This name originates from the fern Pteridium aquilinum var.latiusculum, which is one of the oldest and most common plants in the world and the first plant source from which such compounds were isolated.At the end of the 19th century, reports of lethal intoxication in cattle were established after consuming P. aquilinum [10,11].Pterosin D and Pteroside D were first isolated by Yoshihira et al. [12] from P. aquilunum and have later been found in other fern varieties, such as P. aqullinum subsp.wightianum (Wall) Shich, H. punctata (Thunb) Mett, J. scammanae Tryon, M. speluncae (L.) Moore, M. strigosa (Thunb) Presl, and D. wilfordii (Moore) Christ [13].
Flavonoids are important natural products of ferns.A multitude of these compounds has hitherto been reported from ferns belonging to Dryopteridaceae, Thelypteridaceae, Selaginellaceae, Equisetaceae, Helminthostachyaceae, Ophioglossaceae, Lygodiaceae, The stereochemistry of the chiral carbons belonging to C-3 of the 1-indanone core structure was determined via Circular Dichroism (CD) spectroscopy (Figure S9).The CD spectrum of 14 showed a negative Cotton effect at 301 nm and a positive Cotton effect at 333 nm, which was in accordance with 3R configuration [9].Therefore, compound 14 was identified as 3(R)-O-malonyl pteroside D (Figure 2).A negative molecular ion [M − H] − at m/z 495.1861 corresponding to C 24 H 32 O 11 (calculated: m/z 495.1872; ∆ = −2.17ppm) observed in the high-resolution mass spectrum of compound 14 confirmed this identification (Figure 2 and Figure S8 (in the Supplementary Materials)).
Pterosins are sesquiterpenoids with a 1-indanone core structure; the glucoside version is called a pteroside.This name originates from the fern Pteridium aquilinum var.latiusculum, which is one of the oldest and most common plants in the world and the first plant source from which such compounds were isolated.At the end of the 19th century, reports of lethal intoxication in cattle were established after consuming P. aquilinum [10,11].Pterosin D and Pteroside D were first isolated by Yoshihira et al. [12] from P. aquilunum and have later been found in other fern varieties, such as P. aqullinum subsp.wightianum (Wall) Shich, H. punctata (Thunb) Mett, J. scammanae Tryon, M. speluncae (L.) Moore, M. strigosa (Thunb) Presl, and D. wilfordii (Moore) Christ [13].
Flavonoids are important natural products of ferns.A multitude of these compounds has hitherto been reported from ferns belonging to Dryopteridaceae, Thelypteridaceae, Selaginellaceae, Equisetaceae, Helminthostachyaceae, Ophioglossaceae, Lygodiaceae, Athyriaceae, and Aspleniaceae species [13].In the genus Pteridaceae, the flavonoids of the Pteris species have been extensively investigated.The main flavonoids of ferns in this compound class are flavones, flavonols, and flavanones [14,15].All the flavonoids identified in C. crispa in this paper are flavonols, where the derivatives of quercetin predominate.
In our ongoing research that aims to identify new lead compounds for future anticancer therapy, the cytotoxic activity of four 1-indanone derivatives (compounds 12, 13, 14, and 15) were tested towards the acute myeloid leukaemia cell line MOLM-13.Table 3 presents the EC 50 values after 72 h exposure, which in our previous work, have shown to reveal cytotxic effects at concentrations giving no cell death at 48 h [16].
Moderate and relatively similar cytotoxicity levels towards this cell line were observed for all the compounds, with EC 50 values ranging from 182.88 ± 0.08 µM for compound 12 to 197.88 ± 0.18 µM for compound 14.The cytotoxicity of pterosin D and pteroside D could be regarded with the sugar moiety; meanwhile, adding a substituent in the C-3 position and on C-5 decreases the cytotoxicity of these 1-indanone structures [12,17].
Table 3. Cytotoxicity of compounds 12-15 against three mammalian cell lines.The compounds were dissolved in DMSO.The cells were tested for metabolic activity after 72 h of incubation.The EC 50 values were determined via non-linear regression from 3 independent experiments (MOLM13), as described in the methods section.The data from H9c2 and NRK cells originate from three experiments."-" denotes that no data are available due to low or no observed toxicity above concentrations of 200 µM.

MOLM13 (µM)
H9c2 These compounds exhibited selective cytotoxic activity towards MOLM13, which is illustrated by the fact that none of these compounds were cytotoxic towards the normal cell lines NRK and heart cells, respectively (Table 3).Pterosides are 1-indanone derivatives that are structurally related to ptaquiloside derivatives, which are cancer-promoting agents [18].However, the cancer-promoting effects are normally not observable within a short timescale of 24 to 72 h, which was applied in our experiments to determine the cytotoxicity of individual compounds.McMorris et al. [19], who studied the structure-activity relationship of the structurally related illudins isolated from Omphalotus illudens mushrooms, reported that illudins have a significant anticancer potential.Illudins are structurally related to ptaquiloside structures with fewer cytotoxic effects than ptaquiloside.Some of these compounds, for example, illudin M and illudin S, exhibit significant antibacterial activity [20].The latter-mentioned compounds are more potent than the pterosides described in this paper, with cytotoxic effects against myeloid leukaemia HL 60 cells in the range of 6-100 nM [21].According to Liston and Davis [22], EC 50 concentrations of around 180 µM are within the upper range of biologically relevant anticancer drugs.However, our determinations of cytotoxic activity have been performed on cell cultures; henceforth, the predictions of which concentrations can be achieved in vivo remain elusive.
In comparing the cytotoxicity of the pterosides presented in this work with the other 1-indanone structure, we found that the mild and selective toxicity towards AML MOLM13 cells is a positive discovery worthy of further investigations.

Plant Material
Fresh plant material of Cryptogramma crispa was collected during the summers of 2021 and 2022 on the mountain of Fløyen, in Bergen, Norway, at 302 m above sea level (coordinates 60.397687 N and 005.337902E).A voucher specimen of C. crispa was deposited at the herbarium BG, University of Bergen (accession number BG/S-168787).Before extraction, the fresh plant material was stored at −25 • C for preservation.

Extraction and Partition Purification with Organic Solvents
Aerial parts of Cryptogramma crispa (1.9 kg) were extracted with 22.5 L HPLC-grade methanol (Sigma-Aldrich, St. Louis, MO, USA) for 72 h maceration at room temperature without mixing.The extraction yield was 8.6% of the wet weight.Considering the water content was 94.1%, the dry weight extraction yield was 46.9%.The methanolic extract was percolated through glass wool and concentrated with rotary evaporators under reduced pressure.The resulting concentrated aqueous extract (700 mL) was purified via liquidliquid partition three times with petroleum ether (Petroleum ether-ACS reagent, Sigma-Aldrich, Saint Louis, MO, USA) using a total volume of 4.0 L. The resulting water phase was further purified via liquid-liquid partition three times with ethyl acetate (Ethyl Acetate-ACS reagent ≥ 99.5%, Sigma-Aldrich, Saint Louis, MO, USA) using a total volume of 3.2 L.
The residual aqueous phase and the ethyl acetate phase were individually concentrated with a rotavapor to a volume of 200 mL each.

XAD-7 Absorption Chromatography
The concentrated residual aqueous extract (200 mL) was added to an Amberlite XAD-7 column (column dimensions 50 × 1000 mm, containing 500 g Amberlite ® XAD-7, 20-60 mesh, Sigma-Aldrich, Saint Louis, MO, USA) to remove bulk substances, like sugars, polysaccharides, and free aliphatic amino acids, from the extract when distilled water was used as a mobile phase.Under these solvent conditions, fewer polar and aromatic compounds are absorbed by the XAD-7 column material.The latter compounds are readily eluted from the XAD-7 column when the mobile phase is changed to pure methanol (HPLC grade).The mobile phase gradient consisted of 5.0 L distilled water, followed by 8.0 L methanol.The flow rate was 5 mL/min.This chromatographic separation gave a total of 13 fractions with volumes of 1 L, which were analysed individually via analytical HPLC.The same procedure was conducted with the ethyl acetate phase, whereby 16 fractions were obtained and analysed individually via analytical HPLC.

Preparative HPLC
Individual pure compounds of the fractions from Sephadex LH-20 column chromatography were isolated via preparative HPLC (Thermo Scientific preparative HPLC equipped with a Dionex Ultimate 3000 variable wavelength detector) equipped with a C 18 Ascentis column (column dimensions 250 × 20 mm; 5 µm, spherical particles).A gradient of two solvents was used for elution, consisting of mobile phase A (super distilled water-TFA 99.9:0.1;v/v) and mobile phase B (acetonitrile-TFA 99.9:0.1;v/v) (acetonitrile was used for HPLC, gradient grade, ≥99.9%,Sigma-Aldrich, Saint Louis, USA).The elution profile consisted of isocratic elution with A-B (90:10 v/v) for 4 min, followed by a linear gradient from A-B (90:10 v/v) to A-B (80:20 v/v) for the next 10 min, isocratic elution with A-B (80:20 v/v) for the next 20 min, followed by a linear gradient from A-B (80:20 v/v) to A-B (70:30 v/v) for the next 10 min, followed by isocratic elution with A-B (70:30 v/v) for the next 20 min.
The flow rate was 15 mL/min.Portions of 750 µL were manually injected into the HPLC column and were manually collected based on the peaks that appeared in the online chromatogram recorded at 280 nm.Analytical HPLC was used to analyse the fractions from preparative HPLC separation.Following this strategy, 42.6 mg compound 1, 66.0 mg of compound 2, 1.7 mg of compound 3, 4.6 mg of compound 4, 2.0 mg of compound 5, 1.5 mg of compound 6, 6.5 mg of compound 7, 12.9 mg of compound 8, 5.3 mg of compound 9, 3.9 mg of compound 10, 21.7 mg of compound 11, 36.8 mg of compound 12, 6.3 mg of compound 13, 2.8 mg of compound 14, and 18.4 mg of compound 15 were isolated.

Analytical HPLC
Individual samples were analysed using an Agilent Technologies 1260 Infinity II HPLC instrument equipped with a multidiode array detector, an autoinjector, and a 250 × 4.6 mm, 5 µm SUPELCO analytical Ascentis ® C18 column.HPLC separation was performed according to the method previously published by Nguyen et al. [23].Two solvents were used for elution: mobile phase A (super distilled water-TFA 99.9:0.1;v/v) and mobile phase B (acetonitrile-TFA 99.9:0.1;v/v), with a flow rate of 1 mL/min, and aliquots of 20 µL were injected.The elution profile began with initial conditions of 90% A and 10% B. Gradient elution followed this for 10 min at 14% B, and then isocratic elution from 10 to 14 min.The subsequent gradient conditions were as follows: 16% B at 18 min, 18% B at 22 min, 23% B at 26 min, 28% B at 31 min, and 40% B at 32 min.This was followed by isocratic elution from 32 to 40 min, gradient elution from 40 to 43 min at 10% B, and final isocratic elution from 43 to 46 min at 10% B [23].

Spectroscopy
High-resolution mass spectra were recorded using a JEOL AccuTOF™ JMS T100LC (JEOL Ltd., Tokyo, Japan) instrument fitted with an electrospray ion source operated in positive mode at a resolving power of approximately 6000 FWHM.Mass spectra were recorded over the mass range of 50-2000 m/z.The samples were analysed as methanolic solutions and introduced to the ESI spray chamber with weakly acidified (0.01% HCOOH) acetonitrile (Acetonitrile-for HPLC, gradient grade, ≥99.9%,Sigma-Aldrich, Saint Louis, MO, USA) used as a spray reagent.
UV-Vis absorption spectra were recorded online during analytical HPLC analysis over the 210-600 nm wavelength range in steps of 2 nm.
Circular Dichroism (CD) spectra were recorded at 20 • C with a nitrogen atmosphere using a Jasco J-810 spectropolarimeter (Jasco Products LLC, Oklahoma City, OK, USA) equipped with a Peltier temperature control unit.This instrument was used to analyse compound 14 (2.9 mM) dissolved in 100% methanol (methanol for HPLC, ≥99.9%,Sigma-Aldrich, Saint Louis, MO, USA).The spectrum obtained was the average of 6 scans and a buffer scan with 100% methanol, which was subtracted from the spectrum.The spectrum was scanned from 185 to 400 nm.A 1 mm path-length cell was used during analysis.

Cytotoxicity
Stock solutions were prepared by dissolving pure compounds to a final concentration of 20 mM in DMSO.The normal rat kidney epithelial cells (NRK, ATCC no.: CRL-6509) and rat cardiomyoblasts (H9c2, ATCC no.: CRL-1446) were cultured in DMEM medium supplemented with 10% foetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA).When the cells reached 80% confluence, they were detached in a mild trypsin treatment (0.33 mg/mL trypsin for 5 min at 37 • C), centrifuged (160× g, 4 min), and reseeded in fresh medium to 25% confluence.
The AML cell line MOLM13 (DSMZ no.: ACC554 [24]) was cultured in RPMI-1640 medium enriched with 10% FBS 8mM L-glutamine (Sigma Life Science, London, UK).The cells were kept in suspension cultures at a density of between 150,000 and 700,000 cells/mL.
For the cytotoxicity experiments, the NRK and H9c2 cells were seeded in 96-well tissue culture plates (4000 cells/well, 0.1 mL) and left overnight to attach before adding the compounds.The MOLM13 cells were seeded in 96-well tissue culture plates at 20,000 cells/well in 0.1 mL on the day of the experiment.
The compounds dissolved in DMSO were added to the cells, and the plates were kept overnight before adding the tetrazolium salt WST-1 according to the manufacturer's instructions (Roche Diagnostics GmbH, Mannheim, Germany).The plates were further incubated for two hours before the signal was recorded at 450 nm with a reference at 620 nm.For blank subtraction, only the medium-added WST-1 and plant compounds were used.This procedure was conducted after 24 and 72 h.
After the recording of WST-1, the cells were then fixed with 2% buffered formaldehyde (pH 7.4) with 0.01 mg/mL of the DNA-specific fluorescent dye, Hoechst 33342.The presence of dead (apoptotic or necrotic) cells was verified via differential interference contrast and fluorescence microscopy as previously described by Oftedal et al. and Myhren et al. [25,26].
EC 50 values were determined using four-parameter regression analysis described by Viktorsson et al. [27] using SigmaPlot v14 software (Systat Software Inc., San Jose, CA, USA).

Conclusions
For the first time, 15 natural products, including the previously undescribed compound 3(R)-O-malonyl pteroside D, have been characterised from Cryptogramma crispa (L.) R. Br.The four isolated 1-indanone derivatives were tested for their cytotoxic activity towards the acute myeloid leukaemia cell line MOLM-13, and all these compounds
C H2BC NMR experiment (pulse program: h2bcetgpl3) was performed with 2 to 16 scans, depending on the individual sample concentrations and 256 experiments in F1.The sweep widths were 10 ppm for 1 H and 160 ppm for 13 C.The acquisition time was between 15 min 57 s (NS = 2) and 2 h 4 min 13 s (NS = 16).The 2D 1 H-13 C HSQC-TOCSY NMR experiment (pulse program: hsqcdietgpsisp2) was performed with 2 to 16 scans, depending on the individual sample concentrations and 256 experiments in F1.The sweep widths were 10 ppm for 1 H and 160 ppm for 13 C.The acquisition time was between 21 min 11 s (NS = 2) and 2 h 44 min 42 s (NS = 16).The 2D 1 H-1 H ROESY NMR experiment (pulse program: roesyphpp2) was performed with 8 scans and 256 experiments in F1.The sweep width was 10 ppm.The acquisition time was 1 h 25 min 36 s.The 2D 1 H-1 H COSY NMR experiment (pulse program: cosygpppqf) was performed with 8 scans and 256 experiments in F1.The sweep width was 10 ppm.The acquisition time was 1 h 25 min 36 s.