Research on Diffusible Signal Factor-Mediated Quorum Sensing in Xanthomonas: A Mini-Review

Xanthomonas spp. are important plant pathogens that seriously endanger crop yields and food security. RpfF is a key enzyme that is involved in the synthesis of diffusible signal factor (DSF) signals and predominates in the signaling pathway regulating quorum sensing (QS) in Xanthomonas. Currently, novel RpfF enzyme-based quorum sensing agents have been proposed as a promising strategy for the development of new pesticides. However, few reports are available that comprehensively summarize the progress in this field. Therefore, we provide a comprehensive review of the recent advances in DSF-mediated QS and recently reported inhibitors that are proposed as bactericide candidates to target the RpfF enzyme and control plant bacterial diseases.


Introduction
In the process of disease occurrence, enzymes, which are a class of catalytically active proteins, catalyze the production of regulators in pathological pathways. In particular, the targeting of enzymes is key to the development of novel agricultural agents [1]. The enzyme inhibitors applied in agriculture are mostly pesticides and herbicides (e.g., organophosphate insecticides that target acetylcholinesterase and glyphosate that targets 5-enolpyruvylshikimate 3-phosphate synthase) [2,3]. Meanwhile, research on fungicides has focused on the main enzyme targets involved in energy metabolism and biosynthesis, such as succinate dehydrogenase (SDH) and pyruvate kinase (PK) [4][5][6]. Conversely, agricultural bactericides are extremely limited and less well studied. The design of green agrochemicals using target enzymes has been an important strategy. Therefore, studying new target enzymes would be conducive to the development of new bactericides with high efficiency, low toxicity, and good specificity [7,8].
Xanthomonas spp., which belong to Gram-negative bacteria, are a class of plant pathogens that can infect at least 124 monocotyledonous and 268 dicotyledonous plant species, including a variety of important agricultural crops, causing huge losses in agricultural production [9]. In particular, Xanthomonas oryzae and Xanthomonas campestris are among the top ten phytopathogens of interest in plant molecular pathology [10]. Xanthomonas campestris pv. campestris (Xcc) infects cruciferous plants to cause black rot disease, which is periodically diffused as a worldwide plant disease, and Xanthomonas oryzae pv. oryzae (Xoo) infects rice and causes bacterial leaf disease, leading to rice yield losses ranging from 20% to 80% [11,12]. In addition, Xanthomonas spp. produce virulence factors that strengthen the competitive advantages and enhance the environmental adaptability for host plant infection and bacterial multiplication [13]. The key virulence factors include gered, and the activation of intracellular signaling pathways leads to a coordinated increase in gene expression to produce various virulence factors, including biofilms, extracellular enzymes, phytotoxins, and motility factors, thus providing favorable conditions for the bacteria to infect the host [19].
The most well-known QS systems are mediated by N-acyl homoserine lactones (AHLs), which are the first signaling molecules to be identified in Gram-negative bacteria [20]. Somewhat differently, the QS system for Xanthomonas is regulated by diffusible signal factors (DSFs) rather than AHLs [21]. The QS in Xanthomonas is also associated with rpf (regulation of pathogenicity factor) genes, among which the main genes rpfF, rpfB, rpfC, and rpfG encode proteins that are involved in DSF synthesis, turnover, sensing, and transduction, respectively [22]. Ordinarily, the inhibition of QS occurs via limiting signal accumulation. It is a widely studied approach that involves the degradation and deactivation of signaling molecules, especially in the AHL-mediated mode, such as quorum quenching enzymes [23]. Notably, an alternative approach is the inhibition of synthetase, which is studied less than the former and scarcely reported in DSF-mediated QS. However, some studies indicate that this approach is feasible, effective, and has the potential to inhibit plant pathogen infection processes [24].
The QS pathway is currently being considered as a target for drug design, and there are multiple regulatory proteins that may also participate as possible targets, such as signaling molecular synthases. This review aims to describe the QS pathway in Xanthomonas spp., illustrate the key role of the signaling molecule DSF and that of the biosynthase RpfF, and provide a summary of advancements in the research on RpfF enzymes as potential targets to control plant diseases.  The most well-known QS systems are mediated by N-acyl homoserine lactones (AHLs), which are the first signaling molecules to be identified in Gram-negative bacteria [20]. Somewhat differently, the QS system for Xanthomonas is regulated by diffusible signal factors (DSFs) rather than AHLs [21]. The QS in Xanthomonas is also associated with rpf (regulation of pathogenicity factor) genes, among which the main genes rpfF, rpfB, rpfC, and rpfG encode proteins that are involved in DSF synthesis, turnover, sensing, and transduction, respectively [22]. Ordinarily, the inhibition of QS occurs via limiting signal accumulation. It is a widely studied approach that involves the degradation and deactivation of signaling molecules, especially in the AHL-mediated mode, such as quorum quenching enzymes [23]. Notably, an alternative approach is the inhibition of synthetase, which is studied less than the former and scarcely reported in DSF-mediated QS. However, some studies indicate that this approach is feasible, effective, and has the potential to inhibit plant pathogen infection processes [24].
The QS pathway is currently being considered as a target for drug design, and there are multiple regulatory proteins that may also participate as possible targets, such as signaling molecular synthases. This review aims to describe the QS pathway in Xanthomonas spp., illustrate the key role of the signaling molecule DSF and that of the biosynthase RpfF, and provide a summary of advancements in the research on RpfF enzymes as potential targets to control plant diseases.

The rpf Gene Cluster
The regulation of the rpf gene cluster in relation to virulence factors has been widely studied in Xanthomonas spp. Generally, the rpf cluster of genes consists of at least nine genes (rpfA-rpfI) [25]. It has been reported that rpfABFCHG is in the left-hand section and rpfDEI is in the right-hand section [26]. In Xcc, rpfA, which is adjacent to rpfB and rpfF, is involved in the regulation of pathogenicity factor production and encodes an aconitase that is implicated in iron homeostasis [27]. Instead, rpfB and rpfF are involved in the synthesis of DSF in Xanthomonas. In X. oryzae pv. oryzae PXO99A, mutants with an rpfB deletion can also boost DSF production, and mutant rpfB overexpression can induce the opposite results [28]. In addition to encoding an enoyl-CoA hydratase, which is involved in DSF synthesis, the rpfF gene plays a prominent role in the maintenance of membrane integrity in Xoo by regulating the fatty acid synthesis pathway [29]. The mutant of rpfF exerts a negative influence on the rpfB gene and fatty acyl-CoA ligase (FCL) activity [30]. In Xoo, to define the function of the main rpf genes, rpf mutants were inoculated on rice. With regard to the lesion length, rpfB, C, F, and G had a more significant influence on the pathogenicity than rpfA, D, and I [31]. In Xcc, rpfC and rpfG, respectively, encode two proteins that together compose a two-component system, and rpfH is described as part of the rpfGHC operon, which encodes RpfC/RpfG, a two-component system [25,32]. The mutant rpfC led to the overproduction of DSF and lower levels of EPS and extracellular enzymes. Moreover, the rpfC mutant decreased the expression of hrpX and expressed a weakened phenotype of the hypersensitive response (HR), although the mutant rpfG did not affect hrpX. In other words, hrpX is important for the Type III secretory system (T3SS) and is positively regulated by rpfC, independently of rpfG [33]. Further studies are needed to shed light on the regulatory link between rpf /DSF and hrp/T3SS. Unlike the left-hand section, the right-hand section has fewer studies due to the minor regulatory roles. It has been reported that the mutation of rpfE had no detectable effects on the bacterial interactions with plants and no effects on the production of DSF [34,35]. In 2013, rpfE gene mutant strains were generated to elucidate the role of RpfE with respect to the rpf system in a Korean Xoo KACC10859m gene cluster [34]. The results suggested that the rpfE gene regulates the virulence of Xoo under different nutrient conditions without changes in DSF production.
Rpf-regulated processes have been identified using comparative proteomic findings, indicating changes in the abundance of several functional proteins in the rpfC, rpfG, and rpfF mutants. An analysis of the regulatory influence of individual Rpf proteins supported a previous conclusion based on transcriptomics [36].

DSF-Family Signals
Another representative class of signaling molecules is the QS system of Gram-negative bacteria, which has attracted considerable interest in DSF. In Xcc, the rpf gene clusters related to pathogenicity were initially identified to influence the production of EPS and extracellular enzymes [37]. Interestingly, when the rpf gene clusters were silenced, the production of extracellular enzymes decreased. However, protease and endoglucanase activities can be restored when an rpfF mutant is introduced in proximity to its wild-type parental strain. Therefore, it is speculated that small molecular signals, called DSFs, may induce the synthesis of extracellular proteases associated with rpfF. Moreover, the DSF activity was preliminarily detected using a protease assay, which involved the observation of a clear zone on a skimmed milk agar plate [37]. For further DSF activity detection, DSF biosensor strains have been established, which allow for a semi-quantitative determination [25]. DSF was isolated, purified, and identified from Xcc culture supernatants of rpfC mutants as cis-11-methyl-2-dodecenoic acid [38]. In addition, newly identified DSF-family signals were extracted from Xoo for further improvements in in vitro methods [39], which are as follows: cis-2-dodecenoic acid (BDSF), cis, and cis-11-methyldodeca-2,5-dienoic acid (CDSF). BDSF was originally identified in Burkholderia cenocepacia as being involved in the regulation of motility, biofilm formation, and virulence [40].
DSF-family signals are mostly present in Xanthomonas spp., which does not mean that DSF signals are restricted to Xanthomonas. On the contrary, it allows for essential insight into the broader significance of the DSF-family signaling systems in the bacterial world [41]. DSF signals or structurally related molecules are also produced by unrelated bacteria, such as Burkholderia cenocepacia, Xyllela fastidiosa, and Stenotrophomonas maltophilia, to coordinate expressions of virulence genes [40,42,43]. Ralstonia solanacearum not only produces AHL signals to regulate virulence, but it can also produce signal molecules close to the DSF family, suggesting that Ralstonia solanacearum has two ways to regulate virulence [44]. The human pathogen Pseudomonas aeruginosa can even produce the DSF-like molecule cis-2-decanoic acid to mediate inter-and intraspecies communications [45].
DSF exerts multifaceted effects on the invasion plants. In addition to the regulation of bacterial virulence via intra-communications [39], it has recently been reported that DSF can elicit plant immunity and participate in interspecies communication [46]. Plant actin is an important component of a plant's cytoskeleton, and its assembly can be attenuated due to negative regulation of formin-formin interactions via DSF [47]. Additionally, DSF will be conducive to the competitive capability of Xcc against Bacillus thuringiensis through the modulation of ftsZ in relation to cell division [48]. These findings indicate the significant importance of DSF signaling in the bacterial world.

The QS Pathway in Xanthomonas
DSF-mediated QS has attracted much attention, and the scheme of the regulatory system by and large has been studied [21,49]. Generally, DSF is produced, detected, and transduced by Rpf proteins located upstream of the QS. Synthase RpfF is significant for the biosynthesis of DSF signals, and a hybrid sensor kinase RpfC negatively controls DSF synthesis due to protein-protein interactions with RpfF, which, conversely, can be responsible for DSF perception through H198 autophosphorylation [32]. As shown in Figure 2, at low cell densities, the catalytic activity of RpfF is hindered by its binding to RpfC, while only a few DSF signals are produced by the low levels of free RpfF. In addition, a second messenger, cyclic di-GMP, binds to the global transcription factor (Clp) and prevents the binding of Clp to the promoters of genes encoding virulence factors, including those encoding virulence factors downstream. At high cell densities, RpfC detaches from RpfF and combines with RpfG to form a two-component regulatory system, which is a mechanism of signal perception and transduction. The RpfC sensor can detect the DSF signal that is largely synthesized by the released RpfF, whereas the sensor mechanism is still unclear. Subsequently, the signal is transferred by RpfC to the response regulator RpfG using a phosphorelay mechanism, and RpfG activity as a cyclic di-GMP phosphodiesterase is activated. Finally, the free forms of Clp dominate, and, as a consequence, the decrease in cyclic di-GMP levels drive gene expression.
Molecules 2023, 28, x FOR PEER REVIEW 4 of 12 dienoic acid (CDSF). BDSF was originally identified in Burkholderia cenocepacia as being involved in the regulation of motility, biofilm formation, and virulence [40]. DSF-family signals are mostly present in Xanthomonas spp., which does not mean that DSF signals are restricted to Xanthomonas. On the contrary, it allows for essential insight into the broader significance of the DSF-family signaling systems in the bacterial world [41]. DSF signals or structurally related molecules are also produced by unrelated bacteria, such as Burkholderia cenocepacia, Xyllela fastidiosa, and Stenotrophomonas maltophilia, to coordinate expressions of virulence genes [40,42,43]. Ralstonia solanacearum not only produces AHL signals to regulate virulence, but it can also produce signal molecules close to the DSF family, suggesting that Ralstonia solanacearum has two ways to regulate virulence [44]. The human pathogen Pseudomonas aeruginosa can even produce the DSF-like molecule cis-2-decanoic acid to mediate interand intraspecies communications [45].
DSF exerts multifaceted effects on the invasion plants. In addition to the regulation of bacterial virulence via intra-communications [39], it has recently been reported that DSF can elicit plant immunity and participate in interspecies communication [46]. Plant actin is an important component of a plant's cytoskeleton, and its assembly can be attenuated due to negative regulation of formin-formin interactions via DSF [47]. Additionally, DSF will be conducive to the competitive capability of Xcc against Bacillus thuringiensis through the modulation of ftsZ in relation to cell division [48]. These findings indicate the significant importance of DSF signaling in the bacterial world.

The QS Pathway in Xanthomonas
DSF-mediated QS has attracted much attention, and the scheme of the regulatory system by and large has been studied [21,49]. Generally, DSF is produced, detected, and transduced by Rpf proteins located upstream of the QS. Synthase RpfF is significant for the biosynthesis of DSF signals, and a hybrid sensor kinase RpfC negatively controls DSF synthesis due to protein-protein interactions with RpfF, which, conversely, can be responsible for DSF perception through H198 autophosphorylation [32]. As shown in Figure 2, at low cell densities, the catalytic activity of RpfF is hindered by its binding to RpfC, while only a few DSF signals are produced by the low levels of free RpfF. In addition, a second messenger, cyclic di-GMP, binds to the global transcription factor (Clp) and prevents the binding of Clp to the promoters of genes encoding virulence factors, including those encoding virulence factors downstream. At high cell densities, RpfC detaches from RpfF and combines with RpfG to form a two-component regulatory system, which is a mechanism of signal perception and transduction. The RpfC sensor can detect the DSF signal that is largely synthesized by the released RpfF, whereas the sensor mechanism is still unclear. Subsequently, the signal is transferred by RpfC to the response regulator RpfG using a phosphorelay mechanism, and RpfG activity as a cyclic di-GMP phosphodiesterase is activated. Finally, the free forms of Clp dominate, and, as a consequence, the decrease in cyclic di-GMP levels drive gene expression.

DSF Synthesis
RpfB, a fatty acyl-CoA ligase, was initially thought to be involved in DSF biosynthesis [40]. In the genome, rpfB and rpfF are adjacent, and their mutants can reduce DSF production. Therefore, it was speculated that both genes are important for DSF synthesis. As for the corresponding encoded enzymes, the RpfF enzyme catalyzes the DSF precursor produced by RpfB. Subsequently, RpfB activity is implicated in the turnover of DSF, whereby bacteria can exit from the QS phase [49,50]. At low cell densities, the complex of c-di-GMP and Clp suppresses rpfB expression. However, rpfB is expressed at high cell density concentrations, and RpfB switches to DSF signals. At the transcriptome level, DSF biosynthesis is overproduced in the presence of the rpfC deletion and is reduced in the presence of overexpression of the REC domain [44]. The REC domain blocks the putative substrate from binding to the Lock RpfF protein in an inactive state; thus, RpfC negatively controls DSF production [51].
DSF biosynthesis is associated with FabH, and DSF signals can be modulated by the FabA-FabB pathway during the fatty acid synthesis process [52,53]. Substrates of 3-hydroxyacyl-ACP with different length chains are synthesized as DSF precursors via the fatty acid synthesis cycle and are then catalyzed by the RpfF enzyme to form DSF-family signals, such as DSF, BDSF, and IDSF [22,54]. In particular, two enzyme activities of RpfF have been identified [42]. As illustrated in Figure 3, firstly, RpfF exerts dehydratase activity, converting 3-hydroxyacyl-ACP into cis-2-acyl-ACP. Furthermore, RpfF may also cleave the ACP-thioester bond to release holo-ACP and generate DSF-family signals, further indicating the presence of thioesterase activity. Additionally, the RpfF enzyme in Xyllela fastidiosa has been shown to catalyze the synthesis of DSF through a similar process that involves the following two steps: catalysis of the formation of a double bond and then hydrolysis of the thioester bond [31].

DSF Synthesis
RpfB, a fatty acyl-CoA ligase, was initially thought to be involved in DSF biosynthesis [40]. In the genome, rpfB and rpfF are adjacent, and their mutants can reduce DSF production. Therefore, it was speculated that both genes are important for DSF synthesis. As for the corresponding encoded enzymes, the RpfF enzyme catalyzes the DSF precursor produced by RpfB. Subsequently, RpfB activity is implicated in the turnover of DSF, whereby bacteria can exit from the QS phase [49,50]. At low cell densities, the complex of c-di-GMP and Clp suppresses rpfB expression. However, rpfB is expressed at high cell density concentrations, and RpfB switches to DSF signals. At the transcriptome level, DSF biosynthesis is overproduced in the presence of the rpfC deletion and is reduced in the presence of overexpression of the REC domain [44]. The REC domain blocks the putative substrate from binding to the Lock RpfF protein in an inactive state; thus, RpfC negatively controls DSF production [51].
DSF biosynthesis is associated with FabH, and DSF signals can be modulated by the FabA-FabB pathway during the fatty acid synthesis process [52,53]. Substrates of 3-hydroxyacyl-ACP with different length chains are synthesized as DSF precursors via the fatty acid synthesis cycle and are then catalyzed by the RpfF enzyme to form DSF-family signals, such as DSF, BDSF, and IDSF [22,54]. In particular, two enzyme activities of RpfF have been identified [42]. As illustrated in Figure 3, firstly, RpfF exerts dehydratase activity, converting 3-hydroxyacyl-ACP into cis-2-acyl-ACP. Furthermore, RpfF may also cleave the ACP-thioester bond to release holo-ACP and generate DSF-family signals, further indicating the presence of thioesterase activity. Additionally, the RpfF enzyme in Xyllela fastidiosa has been shown to catalyze the synthesis of DSF through a similar process that involves the following two steps: catalysis of the formation of a double bond and then hydrolysis of the thioester bond [31].
Host plant metabolites, such as sucrose and glucose, may be efficiently utilized to boost DSF signal synthesis to improve the pathogenicity of X. campestris pv. campestris [55]. Meanwhile, after adding salicylic acid, the bacterial RpfB activity increases, leading to DSF signal turnover [56].

RpfF Protein
Understanding the structure and active sites of the RpfF protein will contribute to the development of novel inhibitors. To our knowledge, some studies have examined the crystal structure and performed homology modeling of the RpfF protein.
In 2010, Cheng et al. obtained the crystal structure of the RpfF protein through coexpression of both the RpfF and REC domains of RpfC in E. coli [51]. As illustrated in Figure 4, the RpfF with an N-terminal α/β spiral core domain and a C-terminal α-helical Host plant metabolites, such as sucrose and glucose, may be efficiently utilized to boost DSF signal synthesis to improve the pathogenicity of X. campestris pv. campestris [55]. Meanwhile, after adding salicylic acid, the bacterial RpfB activity increases, leading to DSF signal turnover [56].

RpfF Protein
Understanding the structure and active sites of the RpfF protein will contribute to the development of novel inhibitors. To our knowledge, some studies have examined the crystal structure and performed homology modeling of the RpfF protein.
In 2010, Cheng et al. obtained the crystal structure of the RpfF protein through coexpression of both the RpfF and REC domains of RpfC in E. coli [51]. As illustrated in Figure 4, the RpfF with an N-terminal α/β spiral core domain and a C-terminal α-helical region belongs to the enoyl-CoA hydratase/isomerase family. By means of sequence alignment, it has also been determined that RpfF harbors two conserved glutamate residues, Glu141 and Glu161, corresponding to enoyl-CoA hydratase, but the residues corresponding to the isomerase are not required for catalysis. As a consequence, RpfF is likely a hydratase and not an isomerase. Meanwhile, the important role of the glutamate residues has been shown to involve DSF biosynthesis. cursor molecule. Leu136, Gly137, Gly138, Gly85, and Leu276 may coordinate substrate binding and catalytic activity.
With regard to the research processes in non-Xanthomonas, RpfF proteins are structurally similar to members of the crotonase superfamily and have two highly conserved catalytic glutamate residues located at their active pockets [42]. A crystallization and a preliminary X-ray diffraction characterization of RpfF from Stenotrophomonas maltophilia have been reported [58].
On the basis of the above interesting results, RpfF is an important functional protein in Xanthomonas that plays a key role in DSF-mediated QS and has a highly conserved active site associated with DSF biosynthesis. Its structure is also conserved in various bacteria, and its functions are consistent. Overall, the structural information on the RpfF enzyme has increased its potential as an interesting target and raised the possibility of structure-based drug designs.

RpfF Inhibitors
Research on DSF-mediated QS is of significance for the control of plant diseases. RpfF may represent a potential antibacterial target, but little is known about inhibitors targeting RpfF. Among the current antibacterial studies, Xoo is a major research focus for the plant pathogen Xanthomonas. To date, research into its mechanism has revealed that its inhibitory actions entail a reduction in virulence factors, inhibition of QS-related genes, and suppression of rpfF genes and their encoded enzymes. The reported RpfF inhibitors were displayed in Figure 5 and listed in Table 1.
In 2017, thyme oil was reported to influence virulence factors, such as biofilm and extracellular enzymes, swimming and swarming with unaffected conditions of bacterial In 2012, the 3D structure of the RpfF protein from Xoo was predicted through a homology modeling method in silico using DSF synthase from Xcc as a template [57]. Consistent with previous studies by Cheng et al., the presence of a hydrophobic pocket in RpfF probably represents a docking site for a DSF precursor. Active site prediction studies showed that RpfF has 37 pockets, and, in particular, ID 37 could be a potential cavity, which contains approximately 20 amino acid residues. These significant residues are also highly conserved between RpfF and enoyl-CoA hydratases, including Glu141 and Glu161. The hydrophobic residues Met170 and Trp258 may come into contact with the DSF precursor molecule. Leu136, Gly137, Gly138, Gly85, and Leu276 may coordinate substrate binding and catalytic activity.
With regard to the research processes in non-Xanthomonas, RpfF proteins are structurally similar to members of the crotonase superfamily and have two highly conserved catalytic glutamate residues located at their active pockets [42]. A crystallization and a preliminary X-ray diffraction characterization of RpfF from Stenotrophomonas maltophilia have been reported [58].
On the basis of the above interesting results, RpfF is an important functional protein in Xanthomonas that plays a key role in DSF-mediated QS and has a highly conserved active site associated with DSF biosynthesis. Its structure is also conserved in various bacteria, and its functions are consistent. Overall, the structural information on the RpfF enzyme has increased its potential as an interesting target and raised the possibility of structure-based drug designs.

RpfF Inhibitors
Research on DSF-mediated QS is of significance for the control of plant diseases. RpfF may represent a potential antibacterial target, but little is known about inhibitors targeting RpfF. Among the current antibacterial studies, Xoo is a major research focus for the plant pathogen Xanthomonas. To date, research into its mechanism has revealed that its inhibitory actions entail a reduction in virulence factors, inhibition of QS-related genes, and suppression of rpfF genes and their encoded enzymes. The reported RpfF inhibitors were displayed in Figure 5 and listed in Table 1.

Inhibitor Origin Mechanistic Action Reference
Thymol Major component of Thyme oil Formation of potential hydrogen bonds with some residues Glu161 and Gly169 in the putative RpfF binding pocket; downregulation of rpfF gene; reduction of DSF and BDSF.
Singh et al. [59] Citronellal Major component of Kaffir lime oil Down regulation in transcript levels of rpfF; formed probable hydrogen bonds with important catalytic residues (GLY169, TRP258).

Singh et al. [60]
Chumacin-1 Pseudomonas aeruginosa strain CGK-KS-1 Inhibition of the production of DSF; suppressed the xanthan gum secretion and also inhibited the biofilms formed; interaction with the residues of RpfF pocket. Kanugala

Conclusions
DSF-mediated QS has been newly recognized as a major component of the pathogenicity network in Xanthomonas, and it can be considered a crucial target for suppressing plant bacterial diseases. During the drug design process, enzymes are a class of targets that need to be highly valued. Due to their high specificity, enzymes, which are involved in catalytic reactions associated with disease, can be an important potential target. Therefore, the ability to produce DSF makes RpfF a possible target against Xanthomonas. As for the design of antibacterial agents, the putative substrate-binding pocket and amino acid residues crucial for DSF synthesis also deserve closer attention. Although progress has been made in the exploration of DSF-mediated QS inhibitors in recent years, there are still many unknown active compounds targeting the signal synthetase RpfF that need to be discovered. Currently, the reported inhibitors have been identified as essential oils or derivatives of natural products, which are providing researchers with potential design ideas. More specifically, studying their vital mechanisms in antibacterial activity may make it In 2017, thyme oil was reported to influence virulence factors, such as biofilm and extracellular enzymes, swimming and swarming with unaffected conditions of bacterial growth and metabolic activity under the control of Xoo [59]. Further authenticated by qRT-PCR, thyme oil exerted an inhibitory effect on gene expression, particularly on the rpfF gene. As HPTLC analysis results showed, a significant reduction in the synthesis of DSF and BDSF signaling molecules was observed after treatment with thyme oil. Thus, thymol (molecule 1) was identified as a major component of thyme oil for studies investigating molecular docking with the RpfF protein. Thymol was found to form potential hydrogen bonds with some residues of catalytic importance, such as Glu161 and Gly169, in the putative RpfF binding pocket.
In 2018, kaffir lime oil (KLO) was found to act as an anti-virulence agent in the context of normal bacterial growth for controlling Xoo [60]. Among the major components of KLO, citronella (molecule 2) showed excellent activity for inhibiting biofilm formation, swimming and swarming activities, and extracellular plant cell wall degrading enzymes. Gene expression analysis showed downregulation in transcript levels of genes associated with virulence factors and rpfF. Molecular docking analyses indicated an interaction between citronella and the RpfF protein, in which the molecule formed probable hydrogen bonds with important catalytic residues (GLY169 and TRP258).
In 2019, two bioactive compounds produced by the Pseudomonas aeruginosa strain CGK-KS-1 showed excellent antibacterial activities for Xanthomonas and were named Chumacin-1 (molecule 3) and Chumacin-2 (molecule 4), respectively [61]. Further mechanistic studies revealed that they inhibited the production of a QS signaling factor and suppressed xanthan gum secretion and biofilm formation by various Xanthomonas pathovars. Chumacin-1 and Chumacin-2 produced by the Pseudomonas aeruginosa strain CGK-KS-1 inhibited DSF ion activity in Xoo. The results showed that these extrolites could bind and inhibit RpfF proteins, and their interactions with RpfF exhibited good docking scores and binding energies.
In 2020, Srilatha et al. predicted the 3D structure of the RpfF protein using homology modeling and docked rifampicin analogues within the active site of RpfF by virtual screening in order to identify potent inhibitors [62]. Some of the screened compounds from the ZINC database had higher energies and lower toxicity than the reference compound rifampicin. Compound 5 was predicted as the optimal molecule. Moreover, active site residues of importance were identified, such as HIS-118, HIS-147, THR-148, ARG-179, ASP-207, ARG-240, and THR-244. In the same year, Mishra et al. discovered that silver nanoparticles exerted inhibitory effects on the rpf gene, but the actual biological activities of the compounds were not investigated further [63]. Coumarin derivative Synthesis based on derivation of natural product Disturbed biofilm formation; suppressed bacterial virulence factors and production of DSF; reduced expression of rpfF gene.
Feng et al. [64] In 2022, a series of coumarin derivatives containing isopropanolamine units were designed as anti-QS agents and evaluated for their QS interference ability [64]. After a treatment with compound 6, the results revealed a marked decrease in significant virulence factors, including biofilm formation, bacterial motility, levels of extracellular enzymes, and a notable reduction in DSF signal production and rpfF gene expression.
Based on the above-mentioned studies, the RpfF inhibitors can be divided into the following three categories: natural products, synthetic compounds, and silver inorganic materials. Among the natural products, compounds 1 and 2 were first verified from an oil extract, and their interaction with RpfF was demonstrated by molecular docking studies. Compounds 3 and 4 were isolated from non-Xanthomonas spp. To select new molecules to target the RpfF protein, engineering natural products based on a ramification strategy and high-throughput screening tactics were effective approaches. Compound 5 was selected from 2500 rifampicin analogues. In addition, some antibacterial agents could repress the expression of the rpfF gene or interfere with DSF signals, which indicated that this class of compounds may be potential RpfF inhibitors, such as silver nanoparticles and compound 6.

Conclusions
DSF-mediated QS has been newly recognized as a major component of the pathogenicity network in Xanthomonas, and it can be considered a crucial target for suppressing plant bacterial diseases. During the drug design process, enzymes are a class of targets that need to be highly valued. Due to their high specificity, enzymes, which are involved in catalytic reactions associated with disease, can be an important potential target. Therefore, the ability to produce DSF makes RpfF a possible target against Xanthomonas. As for the design of antibacterial agents, the putative substrate-binding pocket and amino acid residues crucial for DSF synthesis also deserve closer attention. Although progress has been made in the exploration of DSF-mediated QS inhibitors in recent years, there are still many unknown active compounds targeting the signal synthetase RpfF that need to be discovered. Currently, the reported inhibitors have been identified as essential oils or derivatives of natural products, which are providing researchers with potential design ideas. More specifically, studying their vital mechanisms in antibacterial activity may make it possible to target the RpfF protein for the treatment of infections involving plant pathogens, especially with regard to pathogenicity and pesticide resistance.
In the past few years, QS inhibition strategies have made significant progress in the field of pesticides. As the crucial enzyme in the QS system, the signal synthetase RpfF can be regarded as a promising target against Xanthomonas. Although reported RpfF inhibitors are extremely limited, the design and discovery of RpfF inhibitors remain a challenge for the development of new agrochemicals. Therefore, future research should be directed to focus on addressing the following problems: (1) QS for crop protection is highly sought after by researchers, but the underlying molecular mechanisms of DSF-mediated QS are not well understood. (2) The DSF-binding pocket is a crucial target site; however, the challenge of designing effective RpfF inhibitors remains. (3) Although there are some RpfF inhibitors that may provide lead compounds for discovering new active molecules, an effective and diverse screening strategy is still lacking. Using strategies including natural product derivatization, computer-assisted drug design, and biomimetic synthesis may improve the operational efficiency of RpfF-targeted agrochemical development. Overall, the potential role of rpfF and RpfF inhibitor development in crop protection is worthy of continuous exploration by researchers.

Conflicts of Interest:
The authors declare no conflict of interest.