Impact of Low Lithium Concentrations on the Fatty Acids and Elemental Composition of Salvinia natans

The photosynthetic pigments, protein, macro and microelements concentrations, and fatty acids composition of Salvinia natans, a free-floating aquatic plant, were analyzed after exposure to Hoagland nutrient solution containing 1, 3, and 5 mg/L Li. The Li content of Salvinia natans grew exponentially with the Li concentration in the Hoagland nutrient solution. The exposure to Li did not induce significant changes in Na, Mg, K, Cu, and Zn content but enhanced the Ba, Cr, Mn, Ni and Mo absorption in Salvinia natans. The most abundant fatty acids determined in oils extracted from Salvinia natans were C16:0, C18:3(n6), C18:2(n6), and C18:3(n3). The photosynthetic pigments did not change significantly after exposure to Li. In contrast, chlorophyll and protein content decreased, whilst monounsaturated and polyunsaturated fatty acids content increased after the exposure to 1 mg/L Li. The results indicated that Salvinia natans exposed to low Li concentrations may be a good source of minerals, omega 6 and omega 3.


Introduction
Aquatic plants have a fast growth rate, high photosynthetic efficiency, good biomass productivity and high content of protein, starch, sugar, and fat [1]. Based on their characteristics, aquatic plants are a promising feedstock source, suitable for producing bioresources for protein, fish and other animal feed or supplements, soil compost, biofertilizers, biofuels and other value-added products [1].
Salvinia natans (L.) All. (S. natans) is a native species in central and southeastern Europe. It has a high growth rate and reproduction capacity under various environmental stresses. Moreover, it is easy to spread and harvest and can remove organic and inorganic contaminants from aqueous environments [2]. These properties make S. natans ideal as test plants for phytoremediation and biofortification [2]. Moreover, in some countries, S. natans is used as food due to its high nutritional value [3]. In literature, there are several studies on the heavy metals (Cu, Cd and Zn) and Cr(VI) removal from aqueous medium by S. natans [4]. S. natans is also used for cooking oil removal from aqueous solutions [3]. Casas and Matamoros (2022) showed an increase of long-chain compounds in S. natans structure under exposure to different concentrations of micropollutants (naproxen, diclofenac, carbamazepine, and benzotriazole) [5].
Lithium (Li) has different functions in the growth and development of plants. It may have a beneficial biofortification role in plant metabolism. However, it is highly dependent on the plant species. An addition of 5 mg/L of Li was found to increase biomass The C and H content did not differ significantly after the Li exposure. However, a very small increase of the C and H content was observed in the case of S. natans exposed to 1 mg/L Li, compared to the control plants ( Table 1). The S content was below 0.01% in all studied samples. Previous studies revealed that S. natans has a high growth rate and a high biomass production and, therefore, a high nutrient uptake rate in nutrient-rich water [25]. Thus, in the present study, the small variation of the elemental composition of S. natans after the Li treatment showed that a small concentration of Li does not interfere with the S. natans growth and development. Table 1. The elemental composition and protein content of S. natans exposed to various Li The Chl b content increased by 30 to 50% in all plants exposed to Li ( Figure 1). Compared to the control plants (0 mg/L Li), the Car (x + c) content decreased to an undetectable level in the plants exposed to 1 mg/L Li, decreased by 42% in those exposed to 3 mg/L and doubled in the case of the plants exposed to 5 mg/L Li. The correlation analysis showed a high correlation of Li concentration with Chl a (r = 0.74) and Chl b (r = 0.87) and a moderate correlation with Car (x + c) (r = 0.69) content.
Previously, it was reported that the sunflower chlorophyll a and b contents were not affected. Only the carotenoids decreased after Li supplementation (25 mg/L Li) [10]. While other species showed a reduction in the Chl a and b contents after the exposure to Li (in hydroponic conditions), there are species, such as spinach (in soil medium), that showed an increase in the Chl b content after Li exposure [23,24].
The C and H content did not differ significantly after the Li exposure. However, a very small increase of the C and H content was observed in the case of S. natans exposed to 1 mg/L Li, compared to the control plants ( Table 1). The S content was below 0.01% in all studied samples. Previous studies revealed that S. natans has a high growth rate and a high biomass production and, therefore, a high nutrient uptake rate in nutrient-rich water [25]. Thus, in the present study, the small variation of the elemental composition of S. natans after the Li treatment showed that a small concentration of Li does not interfere with the S. natans growth and development. S. natans showed no significant changes in their protein content (%, DW) after the exposure to Li, except for the exposure to 1 mg/L Li, where a decrease of 30% was observed. Therefore, it can be concluded that a low level of Li exposure does not induce additional stress in S. natans. Many other elements were reported to induce the protein oxidation process. Cd and Ni were found to inhibit protein synthesis due to their possible bond with the protein sulfhydryl groups (PSH) [27,28]. Moreover, S. natans exposed to Al presented a protein oxidation rate 2.72 times higher than in the control plants. Exposure of S. natans to toxic compounds resulted in the loss of proteins in functional states due to protein oxidation, decline of protein synthesis and acceleration of protein turnover [29]. Previous studies reported that glutamic acid, aspartic acid and glycine are the most abundant non-essential amino acids, whereas leucine, lysine, arginine and valine are the most abundant essential amino acids in aquatic plants [7,30].
High correlations were found between protein and Chl a (r = 0.88) and Car (x + c) (r = 0.88) contents, while there was no correlation between Chl b and protein contents.

The Li Impact on the Macro and Micro Elements Content in S. natans
The Li content in S. natans was enhanced gradually by increasing the Li concentrations in the Hoagland nutrient solution; the exposed plants accumulated 21.7, 55.4, and 101 mg/kg (DW) Li, compared to the control experiments ( Figure 2).
Fe's contents were 1.9-4.4-fold greater and significantly differed from the control content (0 mg/L Li). Na and Li contents were positively correlated (r = 0.81), indicating a strong relationship. Previous studies showed that Li has both stimulatory and depressive effects on the concentration and uptake of other elements. Naeem et al. (2021) reported a positive correlation between Li and Na in aquatic environments [6]. Moreover, Li was reported as a substitute for other important monovalent cations (like Na and K) in plant cells and as a competitor with the alkali metals in the plant's root uptake [31]. Ryzymski et al. (2017) found that, in the case of medicinal mushrooms, Agrocybe cylindracea and Hericium erinaceus, Li had no significant effect on the uptake of Ca, K, Mg or Na macroelements, after biofortification experiments with Li [32]. Previously, it was reported that Li had a different impact on several plant nutrients, such as Ca and K. However, in the case of lettuce and watercress, the 2 mM Li exposure showed no significant changes in the plants' K concentrations [6]. Moreover, spinach grown in Li-enriched soil medium showed no changes in the Ca, Mg, Na, Mn, Fe Zn and Cu contents, while the K content decreased significantly [6]. High correlations were found between protein and Chl a (r = 0.88) and Car (x + c) (r = 0.88) contents, while there was no correlation between Chl b and protein contents.

The Li Impact on the Macro and Micro Elements Content in S. natans
The Li content in S. natans was enhanced gradually by increasing the Li concentrations in the Hoagland nutrient solution; the exposed plants accumulated 21 Fe's contents were 1.9-4.4-fold greater and significantly differed from the control content (0 mg/L Li). Na and Li contents were positively correlated (r = 0.81), indicating a strong relationship. Previous studies showed that Li has both stimulatory and depressive effects on the concentration and uptake of other elements. Naeem et al. (2021) reported a positive correlation between Li and Na in aquatic environments [6]. Moreover, Li was reported as a substitute for other important monovalent cations (like Na and K) in plant cells and as a competitor with the alkali metals in the plant's root uptake [31]. Ryzymski et al. (2017) found that, in the case of medicinal mushrooms, Agrocybe cylindracea and Hericium erinaceus, Li had no significant effect on the uptake of Ca, K, Mg or Na macroelements, after biofortification experiments with Li [32]. Previously, it was reported that Li had a different impact on several plant nutrients, such as Ca and K. However, in the case of lettuce and watercress, the 2 mM Li exposure showed no significant changes in the plants' K concentrations [6]. Moreover, spinach grown in Li-enriched soil medium showed no changes in the Ca, Mg, Na, Mn, Fe Zn and Cu contents, while the K content decreased significantly [6].
In the present study, it was observed that the treatment with Li induced the uptake of essential (Ba, Mn, Mo) and nonessential (Cr, Ni) elements from the Hoagland nutrient solutions (Figure 2b), compared to the control plants (exposed to 0 mg/L Li). Interestingly, S. natans exposed to 1 mg/L Li showed 2-3.5-fold higher Cr content compared to the plants exposed to 3 or 5 mg/L Li. The same pattern was also noticed in the case of Ni, where S. natans exposed to 1 mg/L Li resulted in 2.5-3.8-fold higher Ni contents than the plants exposed to 3 or 5 mg/L Li. This data suggests that low Li contents may stimulate the absorption of microelements in S. natans. Exposure of S. natans to 5 mg/L Li showed the In the present study, it was observed that the treatment with Li induced the uptake of essential (Ba, Mn, Mo) and nonessential (Cr, Ni) elements from the Hoagland nutrient solutions (Figure 2b), compared to the control plants (exposed to 0 mg/L Li). Interestingly, S. natans exposed to 1 mg/L Li showed 2-3.5-fold higher Cr content compared to the plants exposed to 3 or 5 mg/L Li. The same pattern was also noticed in the case of Ni, where S. natans exposed to 1 mg/L Li resulted in 2.5-3.8-fold higher Ni contents than the plants exposed to 3 or 5 mg/L Li. This data suggests that low Li contents may stimulate the absorption of microelements in S. natans. Exposure of S. natans to 5 mg/L Li showed the highest Mn content. No correlation between the Li and Cr, Ni and Co contents was found, however, moderate to high correlations were found between Li and Ba (r = 0.65) and Mn (r = 0.70). Watanabe et al. (2016) stated that certain vegetable crop species accumulate more nonessential elements than essential macro elements. For example, Chinese chive leaves, contained high concentrations of V, Cr, Ba, Fe and Ni [33].
The plant species generally contain Ni in a very low concentration (0.05-10 mg/kg DW) [34]. Nickel has a key role in enzyme activities, cellular redox state, biochemical and physiological growth responses, and activation of ureas in higher plants [34,35]. Thus, a Ni deficiency reduces the activity of the ureas, resulting in necrosis and chlorosis on leaf tips and older leaves [34]. Ni is required in small quantities in some leguminous crops for nodule growth and activation of hydrogenase enzyme [34]. On the other hand, excess Ni decreases the uptake and transport of elements such as Fe, Cu, Zn, and Mn in many plant species [34].
In the present study, it was observed that S. natans normal micro-and nonessential elements accumulation can be disturbed by Li, and compared to the control plants, the exposure to 1 to 5 mg/L Li resulted in alternation of the Ni and Cr accumulation.

Lithium Impact on S. natans Fatty Acids
The composition of fatty acids of S. natans oil after exposure to different concentrations of Li is presented in Table 2. According to Mubarak et al. (2016), among the pre-treatments used in lipids extraction from S. molesta oil, ultrasonication gives the highest oil yield compared to microwaving, glass grinding, sand grinding, and autoclaving [36]. In the present study, the obtained oils yield was 5 ± 0.4%. The SFAs identified in S. natans oil are: myristic acid (C14:0), palmitic acid (C16:0), heptadecanoic acid (C17:0), stearic acid (C18:0), arachidic acid (C20:0), and lignoceric acid (C24:0). C16:0 is the predominant fatty acid found in all oil samples (approx. 17.3%), followed by arachidic acid (C20:0) (11.9%). Data are expressed as a percentage of total FAMEs and represent mean ± standard deviation (n = 3); nd-the FA was not detected. Values indicated with different letters were significantly different from each other at p ≤ 0.05 levels, whereas the same letters showed no significant differences (p > 0.05).
The MUFAs identified in S. natans are: palmitoleic acid (C16:1(n9)), cis-10-heptadecenoic acid (C17:1) and oleic acids (C18:1(c + t)(n9)). Oleic acid (C18:1(c + t)(n9)) is the most abundant MUFA component. A small trace of C17:1 was found in the control samples. S. natans had considerably greater omega-6 and omega-3 PUFA concentrations, with a high content of C18:3(n-6) (12.81%), C18:2(cis + trans)(n-6) (7.99%), and C18:3(n-3) (6.15%). Among omega-6, linoleic acid ((C18:2(c + t)(n6), PUFA) was the most abundant, followed by arachidonic acid (C20:4(n6)(PUFA). C18:3(n3) is the only fatty acid present in omega-3 PUFA. PUFAs benefit health by protecting the brain and preventing or mitigating cardiovascular disease due to their anti-oxidant, anti-inflammatory, anti-tumoral and anti-coagulant properties. The human body and the fish cannot synthesize two fatty acids, namely C18:2 and C18:3(n3), so they must be supplemented through food [37]. The PUFAs content revealed that S. natans is a good source of omega-6 and omega-3. According to Prado et al., the presence of some metals during the growth of S. natans can cause a change in photosynthesis and respiration, leading to structural alteration and possible PUFA modification [38]. The content of SFAs, MUFAs and PUFAs found in S. natans after exposure to different concentrations of Li is presented in Table 2. The saturated fatty acid content slightly decreased after treatments with different concentrations of Li. It was found that the MUFAs level increased in the treatment with 1 mg/L Li. The PUFAs concentration also increased after the treatments with Li. The content of C18:3(n6) increased after the treatment with 1 mg/L Li and decreased after the treatment with 3 and 5 mg/L Li. The content of C17:0 and C17:1 was found only in control S. natans, while after the treatment with Li, these two compounds were not identified.
Although some plant species can tolerate increased Li concentrations, in general, high concentrations of Li in the substrate used to grow plants were reported to have a negative impact on the roots and leaves [39,40].
The synthesis of PUFAs mainly consisted of the fatty acid synthesis pathway (FAS) and polyketosynthase (PKS) pathway. Desaturase and elongate enzymes play an important role in synthesising monounsaturated fatty acids in plants [41].
Increased Li content can inhibit enzyme activities, reducing plant growth [42]. On the other hand, Li at low concentrations can stimulate plant growth. In this work, low Li amounts were added to improve plant growth. As shown in Table 2, the omega-6 content increased when 1 mg/L Li was added to the Hoagland nutrient solution. Li in plants interacts with enzymes requiring Ca and Mg [10]. Li is a cofactor for the functioning of some enzymes in plants. It may appear as a replacement factor to improve metabolism due to its affinity to enzymes activated by Ca or Mg [13]. However, the beneficial effects of Li on the S. natans fatty acids contents still require further investigation to expand the knowledge related to the dose-dependent response.

Cluster Analysis
A grouping of Na with C18:3(n3) and omega 3 was observed (Figure 3). Alphalinolenic acid (C18:3(n3)) is a short-chain polyunsaturated fatty acid from the omega-3 family, which proves the strong correlation. The Pearson correlation between C18:3(n3) and C16:0(1.0) was very high, as well as with C18:0 (0.97) (Table S2). A high correlation was obtained between C18:2(c + t) and Cu (r = 0.92) and chl b (0.99) and C18:1(c + t) (r = 0.90). This suggests that Cu can enhance the activity of plants to accumulate more Li.  The omega 3/omega 6 ratio showed a moderate correlation only with C16:0, whereas PUFA/MUSA showed a positive correlation with C16:0 and C18:3(n − 3). The same result was reported by Carvalho et al. for Artemisia spp. plant [43]. The presence of essential minerals helps plants grow and maintain their proper physiological properties.
Low Li concentrations (1, 3 and 5 mg/L Li) did not significantly affect the S. natans major element content, such as Na, Mg, K, Ca, Cu and Zn but enhanced the Fe uptake. Exposure to Li had a significant synergetic effect on the uptake and accumulation of minor essential elements, such as Ba, Cr, Mn, Ni and Mo, compared to the control plants. Mo has a major role in Fe metabolism, while Mo and Fe play important roles in symbiotic nitrogen fixation during plant growth and in different life stages to full maturity [44]. According to Wysokinski et al. (2022) [44], the bioaccumulation factors of Mo and Fe in peas (Pisum sativum L.) are the highest in the initial growth stages. These results are consistent with the current study, indicating that Li can enhance Fe and Mo accumulation in S. natans (in the growing period of 30-40 days) and may help the plants' metabolism in symbiotic nitrogen fixation.

FT-IR Analysis
The FT-IR spectra (Figure 4) of S. natans, after exposure to 0, 1, 3 and 5 mg/L Li, were similar and indicated that the addition of Li had no negative effects on the structure and composition of S. natans. The strong band at 3340-3380 cm −1 was attributed to the O-H stretching in phenols, alcohols, or alkaloids. The bands around 2920 and 2850 cm −1 are specific to aliphatic C-H stretching vibrations [45]. The large band observed at 1632-1658 cm −1 is specific to the aromatic C=C vibrations [46]. The small peak in 1733-1734 cm −1 could be attributed to the C=O stretching vibration of lignin, whereas the peak at 1519-1523 cm −1 was due to the aromatic skeletal vibrations of the lignocellulose contents. The peaks at 1412-1441 cm −1 and 1378-1384 cm −1 were attributed to stretching vibrations of guaiacyl rings and C-O in lignin [46]. The large peak at 1062-1068 cm −1 was attributed to the C-O stretch in polysaccharides, cellulose, and hemi-cellulose, while a large, broad band at 556-569 cm −1 to C=O bend vibrations [47].

Experimental Design
S. natans aquatic plants were obtained from a local aquarium store in Cluj-Napoca, Romania and were grown and multiplied for 30-40 days at a temperature of 19-24 °C, The strong band at 3340-3380 cm −1 was attributed to the O-H stretching in phenols, alcohols, or alkaloids. The bands around 2920 and 2850 cm −1 are specific to aliphatic C-H stretching vibrations [45]. The large band observed at 1632-1658 cm −1 is specific to the aromatic C=C vibrations [46]. The small peak in 1733-1734 cm −1 could be attributed to the C=O stretching vibration of lignin, whereas the peak at 1519-1523 cm −1 was due to the aromatic skeletal vibrations of the lignocellulose contents. The peaks at 1412-1441 cm −1 and 1378-1384 cm −1 were attributed to stretching vibrations of guaiacyl rings and C-O in lignin [46]. The large peak at 1062-1068 cm −1 was attributed to the C-O stretch in polysaccharides, cellulose, and hemi-cellulose, while a large, broad band at 556-569 cm −1 to C=O bend vibrations [47].

Experimental Design
S. natans aquatic plants were obtained from a local aquarium store in Cluj-Napoca, Romania and were grown and multiplied for 30-40 days at a temperature of 19 [45], supplemented with 0.10 µM BaCl 2 , 0.08 µM NiSO 4 ·6H 2 O, 0.08 µM Cr 2 (SO 4 ) 3 , and 0.04 µM CoCl 2 ·6H 2 O. The nutrient solutions were supplemented with low concentrations of non-essential elements (Ba, Ni, Co, and Cr), which water could contain in trace concentrations due to natural sources. For the experiments, plants with a growing age of 30-40 days were used. S. natans were exposed to Hoagland solution enriched with 1, 3 and 5 mg/L Li, prepared from a 1 g/L stock solution, obtained by dissolving LiSO 4 ·H 2 O in Hoagland nutrient solution. The plants were exposed to Li-enriched solutions [45] for seven days under laboratory conditions, following the method of Torok et al. (2022). For the control experiments, a Hoagland nutrient solution without Li addition was used (0 mg/L Li).

Elemental Composition
After seven days of exposure to different Li concentrations, the S. natans plants were washed, freeze-dried using a Labconco FreeZone 2.5 L system (Labconco, Kansas City, MO, USA) and ground to powder. Fifty mg sample was digested with 5 mL of 65% HNO 3 and 2 mL of 30% H 2 O 2 in a closed-vessel Speedwave XPERT (Berghof, Eningen, Germany) microwave digestion system. After cooling, the digested samples were diluted with ultrapure water to a final volume of 20 mL. The measured metal concentrations were expressed as mg/kg dry weight (DW). The Na, Mg, K, Ca, Fe Mn, Cu, Zn and Li concentrations were measured using an Optima 5300 DV inductively coupled plasma-atomic emission spectrometer (ICP-OES, Perkin Elmer, Waltham, MA, USA), while the Ba, Cr, Mn, Co, Ni, and Mo concentrations were determined using an ELAN DRC II inductively coupled plasma-mass spectrometer (ICP-MS, Perkin Elmer, Waltham, MA, USA). The instruments were calibrated using ICP multi-element standard solution IV, 1000 mg/L (Merck, Darmstadt, Germany), and multi-element Calibration Standard 3 (Perkin Elmer Pure Plus, Waltham, MA, USA), while the measurement accuracy was tested using the standard reference material, 1643f NIST-Trace elements in water (National Institute of Standards and Technology, Gaithersburg, MD, USA) and NIM-GWB 10019 Apple-Trace elements (Institute of Geophysical and Geochemical Exploration, Langfang City, China). The detection limit of each element measured by ICP-OES and ICP-MS are presented in Table S1. The average recoveries ranged between 93-104%.

Photosynthetic Pigments Content Determination
The S. natans plants' photosynthetic pigments (chlorophyll a-Chl a, chlorophyll b-Chl b, and carotenoids-Car (x + c)) were extracted using 0.5 g fresh weight (FW) plant biomass in 5 mL of methanol. The plant-methanol mixture was vortexed thoroughly for 1 min. and incubated at 70 • C for 3 min. The extracts were centrifuged, and the absorbance of the supernatant was measured at 665, 652, and 470 nm using a Nanodrop One Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). The content of Chl a, Chl b, and Car (x + c) pigments was calculated according to Lichtenthaler (1987), and the results were expressed as µg/g (FW) [48].

Oil Extraction from S. natans
For the determination of fatty acids in S. natans, the oil was extracted using the green ultrasonic-assisted extraction method of Gasparini et al. (2023) [22] with slight modifications, as follows: 0.5 g of dried plant sample (DW) were extracted by sonication with 25 mL chloroform: methanol (2:1, v/v), using an ultrasonic bath (Bandelin Sonorex, Berlin, Germany) at 25 • C for 1 h. The biomass was separated by filtration, and the extract phase was treated with 10 mL 0.74% KCl solution. The organic phase was separated by filtration using Na 2 SO 4 for water removal. The solvent was removed using a rotary evaporator Laborota 4010 (Heidolph, Schwabach, Germany). The obtained oil was weighed, and the oil yield was calculated. Then, the transmethylation method under alkali-catalysed conditions was applied to the obtained oil samples to convert the glycerides of the fatty acids into the corresponding methyl esters, according to Senila et al. (2020) [49]. The samples (0.06 g) were dissolved in isooctane, treated with 0.2 mL 2 M KOH solution in methanol, and vigorously stirred for 30 s. Finally, the mixture was treated with 1 g NaHSO 4 to prevent the methyl esters' saponification and to neutralize the excess alkali. Each oil sample was trimethylated and analysed in three replicates.

Instrumentation and Chromatographic Conditions
The FAMEs content was determined by GC-FID (Agilent Technologies, 6890N GC, Santa Clara, CA, USA) equipped with a ZB-WAX capillary column (30 m × 0.25 mm × 0.25 µm) and a flame ionization detector (FID, Agilent Technologies 7683, Santa Clara, CA, USA). The gas carrier was helium (purity ≥ 99.999%) with a constant 1 mL/min flow rate. The injection volume was 1 µL in 1:20 split mode. The GC oven temperature program consists of three stages: 60 • C for 1 min, 60 to 200 • C (rate 10 • C/min, kept for 2 min), from 200 to 220 • C (rate 5 • C/min, kept for 20 min). The temperature of the injector and detector was set to 250 • C. The data processing was performed with the Agilent OpenLab CDS Chemstation software C.01.10 running under Windows (Microsoft, Santa Clara, CA, USA). The FAs in samples were identified by comparing their retention time with that of the Supelco FAME standard mixtures standard mixture.

FT-IR Analysis
The Fourier transform infrared (FT-IR) spectra of powdered plant samples were recorded between 400-4000 cm −1 on KBr pellets containing 1% sample using a Spectrum BX II (Perkin Elmer, Waltham, MA, USA) spectrometer.

Statistical Analysis
The graphical processing of data and statistical analysis were performed using Origin-Lab (version 2020b, Northampton, MA, USA) software. All data were expressed as mean ± standard error, and the differences between the studied parameters were tested by comparing the averages of the three replicates using Tukey's test for a significance level of p = 0.05 using Paired Comparison App (Two-Way ANOVA).

Conclusions
S. natans aquatic plants grown and multiplied under laboratory conditions were exposed to Hoagland solutions enriched with Li concentrations of 1, 3 and 5 mg/L Li to assess the plant's metabolism. Regarding photosynthetic pigment content, only moderate differences were noted after exposure to Li. S. natanss total protein content revealed no significant changes, except for a 30% decrease after the exposure to 1 mg/L Li. The Li treatment induced a significant Ba, Cr, Mn, Ni and Mo absorption in plants compared to the control plants (exposed to 0 mg/L Li). In contrast, Li accumulation had no significant effect on the macroelements content (Na, Mg, K, Cu and Zn) of S. natans. All the samples presented saturated, monounsaturated, and polyunsaturated fatty acids. The most abundant fatty acids were C16:0, C18:3(n6), C18:2(n6), and C18:3(n3). FT-IR spectra did not reveal changes in the composition of S. natans exposed to various Li concentrations. The results indicate that S. natans is an effective source of minerals, omega 6 and omega 3 fatty acids. Data from the current study provide valuable information on the influence of Li on the fatty acids and elemental composition of S. natans. However, the analyses were limited to low Li concentrations. Thus, further experiments on the exposure to a wider spectrum of Li concentrations and other elements may be of interest, providing additional information on the potential application of S. natans in various supplements. Increasing the use of this abundant and valuable aquatic plant in different industries would be a promising solution for the food industry and for the remediation of environmental pollution.

Data Availability Statement:
The data presented in this study is available upon request from the corresponding author.