Supramolecular Crystal Networks Constructed from Cucurbit[8]uril with Two Naphthyl Groups

Naphthyl groups are widely used as building blocks for the self-assembly of supramolecular crystal networks. Host–guest complexation of cucurbit[8]uril (Q[8]) with two guests NapA and Nap1 in both aqueous solution and solid state has been fully investigated. Experimental data indicated that double guests resided within the cavity of Q[8], generating highly stable homoternary complexes NapA2@Q[8] and Nap12@Q[8]. Meanwhile, the strong hydrogen-bonding and π···π interaction play critical roles in the formation of 1D supramolecular chain, as well as 2D and 3D networks in solid state.


Introduction
Supramolecular self-assembly is a spontaneous process in which basic structural units form ordered structures driven by non-covalent interactions (including electrostatic interactions, van der Waals forces, hydrogen bond, π-π stacking, hydrophobic effects, etc.) [1][2][3][4][5][6]. Based on self-assembly, various supramolecular network structures have been widely developed. Reversible non-covalent bonds endow supramolecular networks with traditional polymer properties, as well as dynamic properties, which can achieve high response to stimuli and self-healing functions [7][8][9][10]. These supramolecular network systems have been constructed to synthesize different functional materials and widely used in drug carriers, nano containers, molecular devices, sensors, adsorption and separation materials, catalysts, and environmental pollutant treatment [11][12][13][14][15]. Although the supramolecular polymerization process has been deeply understood, it is still a huge challenge to design controllable supramolecular polymerization systems and provide supramolecular polymers with controllable structures [16].
The macrocyclic host cucurbit[n]urils (Q[n]s, CB[n]s, n = number of glycoluril units) are spherical macrocyclic compounds adopting a stable rigid structure and composed of n glycoluril units bridged by 2n methylene groups with a hydrophobic cavity [17][18][19][20]. As a kind of rigid macrocyclic host, cucurbit[n]urils have three main structural characteristics, namely, the neutral cavity, the negative electrostatic potential carbonyl portals and the positive electrostatic potential of the outer surface [21,22]. Based on the above three characteristics, the Q[n]s related host-guest chemistry, coordination chemistry and outer surface interaction chemistry have been widely used [23][24][25]. The cavity of the Q[n]s can selectively bind a part of or whole guest molecules to form host-guest complexes [26][27][28][29]. According to the Q[n]'s cavity size, Q [8] exhibits unique molecular recognition characteristics enabling dimerization of specific guest molecules inside the cavity of Q [8] in a controlled manner. The larger cavity of Q [8] can bind two aromatic group, such as phenyl, naphthyl, indole, quinoline, and larger aromatic rings, thus allowing for the formation of homoternary inclusion complexes [30][31][32]. Therefore, Q [8] has been vigorously studied as basic units for the construction of supramolecular networks [33][34][35]. For example, Zhang's team constructed a variety of supramolecular hyperbranched networks by self-assembly of dendrimers and Q [8] [36,37]. Liu's team constructed a variety of two-dimensional supramolecular network structures based on triphenylamine and Q [8] [38,39], which have good effects in many fields such as cell imaging, near-infrared lysosome targeted imaging. Li's team prepared a tetrahedral molecule which was used to co-assemble with Q [8] to afford a new water soluble 3D diamondoid system [40]. Moreover, the hexa-armed [Ru(bpy) 3 ] 2+ -based derivatives and Q [8] were used to afford another 3D cubelike system, which could be used as heterogeneous catalyst for hydrogen production and organic reaction [41]. In previous work, we used the antiparallel encapsulation of styrene pyridine dimer in Q [8] to construct a variety of supramolecular polymer systems [42,43]. For example, we generated an irreversible covalent component for the construction of the first highly watersoluble 3D supramolecular-covalent organic framework, which was used to highly promote the electron transfer of protons to H 2 when loaded on POM catalysts and Ru 2+ -complex photosensitizers [42]. Having said that, we believe that Q[n]s are ideal as basic building blocks for the construction of Q[n]-based networks.

Molecular Binding Behavior and Thermodynamic between Cucurbit[8]uril and NapA or Nap1
It has been established that Q [8] encapsulation for the dimers of naphthyl unit in water can remarkably promote the π-π interaction. Thus, two guests containing naphthyl unit (NapA and Nap1) were designed and synthesized (Scheme 1). Two guests, NapA and Nap1, were prepared in a one-step sequence. Compound NapA was prepared from the reaction of 4-(4-pyridyl)benzoic acid and 2-bromomethyl naphthalene in MeCN in 90% yield and characterized using 1 H NMR, 13 C NMR, 1 H-1 H COSY spectra and highresolution mass spectra (HR-MS). 4,4 -bipyridine and 2-bromomethyl naphthalene were used to synthesize Nap1 in acetone in 85% yield. We then studied their coassembly with Q [8] in water for the formation of two new homoternary supramolecular complexes.  The naphthyl units of two guests can combine to form supramolecular dimers under the action of Q[8]-driven self-assembly. Two examples of homoternary supramolecular complexes were constructed under the coordination of supramolecular interactions such as Q[n]s' outer surface interaction, hydrogen bond interaction, strong π-π interaction, hostguest interaction, etc. Simply mixing Q [8] and Nap1 or NapA in aqueous solution resulted in the formation of the supramolecular networks depending on the corresponding hostguest interaction between the two species of molecules, which was further characterized by 1 H NMR spectroscopy, X-ray single crystal diffraction, and dynamic light scattering analysis (DLS).
The 1 H NMR spectroscopy measurements indicated that both Nap1 and NapA form host-guest inclusion complexes with Q [8] host. In the presence of small amount of the Q [8] hosts (Figure 1), the signals of both free and complexed guests were simultaneously observed and were very broad, indicating slow exchange of free and complexed guests on the NMR time scale. On the one hand, the protons of bipyridine (H 1 -H 4 ) and one of the CH 2 protons (H 5 ) of Nap1 moved downfield slightly, which indicated that they were located outside the cavity. On the other hand, at a 2:1 ratio of Nap1 to Q [8], the naphthyl peaks (H 6 -H 12 ) were completely shifted upfield. These observations suggested that the naphthyl moiety of the Nap1 guest was encapsulated into the cavity of the Q [8] host. Isothermal titration calorimetry (ITC) was also employed to afford the thermodynamic parameters of Q [8] with both Nap1 and NapA. ITC experimental data further confirmed that the binding stoichiometry of Q [8] to both Nap1 and NapA is 1:2 ( Figure  2). From the ΔH and TΔS values in Table S1, it was clear that the formation of both homoternary complexes were enthalpically driven. The observed negative enthalpy change (ΔH = −36.17 ± 2.59 kJ·mol −1 for NapA2@Q [8]; ΔH = −22.71 ± 1.37 kJ·mol −1 for Nap12@Q [8]) were probably due to the cooperativity of above mentioned four kinds of weak interactions. On the basis of the corresponding experimental results, we also obtained the association constants of Ka = (2.14 ± 0.62) × 10 10 M −2 and (1.48 ± 0.45) × 10 10 M −2 for Q [8] with NapA and Nap1, respectively, which was much larger than that of Q [8] with tripeptides reported by Urbach [44] and closer to Q [8] with tripeptides reported by us [30]. Such a high binding constant suggested the relatively strong host-guest interaction between Q [8] and NapA or Nap1, indicating the construction of stable homoternary complexes NapA2@Q [8] and Nap12@Q [8] in aqueous solution.  The corresponding 1 H NMR titration spectra of the Q [8] with NapA were showed in Figure S1. Due to the low solubility of NapA, the NMR signals became wide peak after adding Q [8]. In the presence of 0.5 equiv of Q [8], the signals corresponding to the naphthyl (H 6 -H 12 ) protons of the NapA shifted upfield, while those corresponding to the pyridinyl (H 3 and H 4 ), phenyl (H 1 and H 2 ) protons did not move significantly. This clearly indicated that the naphthyl protons of the guest NapA were buried inside the hydrophobic cavity of Q [8], while the 4-(4-pyridyl)benzoic acid moiety resided outside of the Q[8] portals, forming homoternary supramolecular complexes with 1:2 host-guest binding ratio NapA 2 @Q [8]. By comparing the NMR titration data of the two self-assemblies, we found that the naphthyl group of the guest molecule was encapsulated in the hydrophobic cavity of Q [8].
Isothermal titration calorimetry (ITC) was also employed to afford the thermodynamic parameters of Q [8] with both Nap1 and NapA. ITC experimental data further confirmed that the binding stoichiometry of Q [8] to both Nap1 and NapA is 1:2 ( Figure 2). From the ∆H and T∆S values in Table S1, it was clear that the formation of both homoternary complexes were enthalpically driven. The observed negative enthalpy change (∆H = −36.17 ± 2.59 kJ·mol −1 for NapA 2 @Q [8]; ∆H = −22.71 ± 1.37 kJ·mol−1 for Nap1 2 @Q [8]) were probably due to the cooperativity of above mentioned four kinds of weak interactions. On the basis of the corresponding experimental results, we also obtained the association constants of K a = (2.14 ± 0.62) × 10 10 M −2 and (1.48 ± 0.45) × 10 10 M −2 for Q [8] with NapA and Nap1, respectively, which was much larger than that of Q [8] with tripeptides reported by Urbach [44] and closer to Q [8] with tripeptides reported by us [30]. Such a high binding constant suggested the relatively strong host-guest interaction between Q [8] and NapA or Nap1, indicating the construction of stable homoternary complexes NapA 2 @Q [8] and Nap1 2 @Q [8] in aqueous solution. Isothermal titration calorimetry (ITC) was also employed to afford the thermodynamic parameters of Q [8] with both Nap1 and NapA. ITC experimental data further confirmed that the binding stoichiometry of Q [8] to both Nap1 and NapA is 1:2 ( Figure  2). From the ΔH and TΔS values in Table S1, it was clear that the formation of both homoternary complexes were enthalpically driven. The observed negative enthalpy change (ΔH = −36.17 ± 2.59 kJ·mol −1 for NapA2@Q [8]; ΔH = −22.71 ± 1.37 kJ·mol −1 for Nap12@Q [8]) were probably due to the cooperativity of above mentioned four kinds of weak interactions. On the basis of the corresponding experimental results, we also obtained the association constants of Ka = (2.14 ± 0.62) × 10 10 M −2 and (1.48 ± 0.45) × 10 10 M −2 for Q [8] with NapA and Nap1, respectively, which was much larger than that of Q [8] with tripeptides reported by Urbach [44] and closer to Q [8] with tripeptides reported by us [30]. Such a high binding constant suggested the relatively strong host-guest interaction between Q [8] and NapA or Nap1, indicating the construction of stable homoternary complexes NapA2@Q [8] and Nap12@Q [8] in aqueous solution. To better understand the host-guest interaction between Q [8] and both Nap1 and NapA in aqueous solution, we also carried out UV-vis titration experiments. According to the UV-vis absorption spectroscopic results, as shown in Figure 3, the compound Nap1 and NapA displayed an absorption band at 224 nm belonging to naphthyl unit, which decreased markedly in its intensity upon addition of Q [8], due to the strong interaction between Q [8] and naphthyl moiety of guest molecules Nap1 and NapA. When 0.5 equivalent Q [8] was added, the UV−vis absorption spectra intensity did not change significantly. Their Job plots (based on the continuous variation method) clearly showed that UV−vis spectra data of both Nap1 and NapA fitted well to 1:2 stoichiometry of the host-guest inclusion complexes (Figure 3, inset). The binding stoichiometry was also determined by the Job plot analysis at a fixed total concentration of host and guest molecules. The absorption intensity changes (ΔA) were plotted against the molar fraction of Nap1 and NapA to give a peak at a molar fraction of 0.66, indicating a 1:2 stoichiometry for the Q [8]:Nap1 or Q [8]:NapA inclusion complex ( Figures S2 and S3), which was consistent with the NMR titration results. To better understand the host-guest interaction between Q [8] and both Nap1 and NapA in aqueous solution, we also carried out UV-vis titration experiments. According to the UV-vis absorption spectroscopic results, as shown in Figure 3, the compound Nap1 and NapA displayed an absorption band at 224 nm belonging to naphthyl unit, which decreased markedly in its intensity upon addition of Q [8], due to the strong interaction between Q [8] and naphthyl moiety of guest molecules Nap1 and NapA. When 0.5 equivalent Q [8] was added, the UV−vis absorption spectra intensity did not change significantly. Their Job plots (based on the continuous variation method) clearly showed that UV−vis spectra data of both Nap1 and NapA fitted well to 1:2 stoichiometry of the host-guest inclusion complexes (Figure 3, inset). The binding stoichiometry was also determined by the Job plot analysis at a fixed total concentration of host and guest molecules. The absorption intensity changes (∆A) were plotted against the molar fraction of Nap1 and NapA to give a peak at a molar fraction of 0.66, indicating a 1:2 stoichiometry for the Q [8]:Nap1 or Q [8]:NapA inclusion complex (Figures S2 and S3), which was consistent with the NMR titration results. spectra data of both Nap1 and NapA fitted well to 1:2 stoichiometry of the host-guest inclusion complexes (Figure 3, inset). The binding stoichiometry was also determined by the Job plot analysis at a fixed total concentration of host and guest molecules. The absorption intensity changes (ΔA) were plotted against the molar fraction of Nap1 and NapA to give a peak at a molar fraction of 0.66, indicating a 1:2 stoichiometry for the Q [8]:Nap1 or Q [8]:NapA inclusion complex ( Figures S2 and S3), which was consistent with the NMR titration results. DLS experiments in dilute solutions can be used to monitor the formation of supramolecular networks. DLS results revealed that NapA, Nap1 and Q [8] formed nanoscaled assemblies in water. As can be found, the hydrodynamic diameter (DH) of NapA monomer (1.0 mM) was determined to be 0.6 nm. In Figure 4a  DLS experiments in dilute solutions can be used to monitor the formation of supramolecular networks. DLS results revealed that NapA, Nap1 and Q [8] formed nanoscaled assemblies in water. As can be found, the hydrodynamic diameter (D H ) of NapA monomer (1.0 mM) was determined to be 0.6 nm. In Figure 4a, mixing Q [8] and NapA (1:2, [NapA] = 0.1 mM) observed one hydrodynamic diameter distribution centered at 140 nm. It showed that aggregates of this size were formed in the aqueous solution. The D H value decreased with the dilution of the solution. However, even at [NapA] = 25 µM, D H was still as high as 43 nm. These observations supported that NapA and Q [8] coassembled into the nanoscaled supramolecular networks in water. At the same concentration, the hydrated particle size of assembly NapA 2 @Q [8] is obviously higher than that of assembly Nap1 2 @Q [8], which indicates that the polymerization ability of assembly NapA 2 @Q [8] is higher than that of assembly Nap1 2 @Q [8] in the aqueous phase. DLS experiment showed that the self-assembly had a certain stability in the solution even at low concentration. The result was consistent with the results of crystal structure description. The DLS experiment of Nap1 2 @Q[8] (Figure 4b) was similar to that of NapA 2 @Q [8]. the nanoscaled supramolecular networks in water. At the same concentration, the hydrated particle size of assembly NapA2@Q [8] is obviously higher than that of assembly Nap12@Q [8], which indicates that the polymerization ability of assembly NapA2@Q [8] is higher than that of assembly Nap12@Q [8] in the aqueous phase. DLS experiment showed that the self-assembly had a certain stability in the solution even at low concentration. The result was consistent with the results of crystal structure description. The DLS experiment of Nap12@Q[8] (Figure 4b) was similar to that of NapA2@Q [8].

Single-Crystal X-ray Crystallography
X-ray structure analysis provided unequivocal proof of the formation of homoternary complexes between Q[8] and both Nap1 and NapA. Crystal of NapA2@Q [8] was grown by slow evaporation of a solution containing the host Q [8] and the guest NapA under 3.0 M aqueous hydrochloric acid solution. X-ray structural analysis previously established that the NapA2@Q[8] crystallized in the orthorhombic crystal system, space group Pbca. As can be seen in Figure 5a, the naphthyl moiety of the NapA guest located inside the cavity of the Q [8] host, which was in agreement with what we had observed in the aqueous solution by 1 H NMR spectroscopy. Furthermore, the π···π interactions between two encapsulated NapA molecules played a critical role in the formation of this host-guest inclusion complex. Obviously, the van der Waals contacted between the naphthyl groups and the inner wall of the Q[8] cavity, and strong hydrogen-bonding, such as C(32)-H···O(6) 2.701 Å (between carbonyl oxygen of host and H on benzene ring of guest),  . DLS profile of (a) NapA 2 @Q [8] and (b) Nap1 2 @Q [8] in water at 25 • C. The concentration represents that of NapA or Nap1 of homoternary complexes.

Single-Crystal X-ray Crystallography
X-ray structure analysis provided unequivocal proof of the formation of homoternary complexes between Q [8] and both Nap1 and NapA. Crystal of NapA 2 @Q [8] was grown by slow evaporation of a solution containing the host Q [8] and the guest NapA under 3.0 M aqueous hydrochloric acid solution. X-ray structural analysis previously established that the NapA 2 @Q [8] crystallized in the orthorhombic crystal system, space group Pbca. As can be seen in Figure 5a, the naphthyl moiety of the NapA guest located inside the cavity of the Q [8] host, which was in agreement with what we had observed in the aqueous solution by 1 H NMR spectroscopy. Furthermore, the π···π interactions between two encapsulated NapA molecules played a critical role in the formation of this host-guest inclusion complex. Obviously, the van der Waals contacted between the naphthyl groups and the inner wall of the Q [8] cavity, and strong hydrogen-bonding, such as C(32)-H···O(6) 2.701 Å (between carbonyl oxygen of host and H on benzene ring of guest), contributed to the formation of the inclusion complex NapA 2 @Q [8]. As shown in Figure 5b, two adjacent complexes formed a two dimensional assembled host-guest supramolecular network via hydrogenbonding interactions between the portal carbonyl oxygen atom O6 of Q [8] and between the carboxyl oxygen atom O9, O10 of NapA. It is should be noted that the hydrogen bonds between the carboxyl oxygen atom of the guest NapA and the hydrogen atom on methylene of Q [8] (Figure 5b Single crystals of complex Nap12@Q [8] were fortunately obtained from hydrochloride acid solution by slow evaporation in the presence of CdCl2. The complex crystallized in the triclinic crystal system, space group P-1, the compounds consists of one Q [8] host and two Nap1 guests. As shown in Figure 6a, X-ray structural analysis revealed that the naphthyl moiety of the guest Nap1 adopted reverse parallel in the Q [8] cavity, the bipyridyl moiety remained outside of its portal, which could be attributed to π···π interactions in the cavity of Q [8]. Outside of the inclusion complexes, neighboring Nap1 molecules contacted with each other through not only π···π interaction, but also C-H···π interactions (Figure 6b), which may serve to stabilize this structure for building a one-dimensional supramolecular chain of the complexes. In the latter, the encapsulated guests were electron donor and acceptor pair, and the major driving force for the ternary complex formation appears to be strong charge-transfer interaction between the guests [45]. Furthermore, the strong hydrogen-bonding played a critical role in the formation of this hostguest inclusion complex, e.g., between H on methylene of Nap1 and the carbonyl oxygens at the portals of the Q [8] host C(59)-H···O (6)   Single crystals of complex Nap1 2 @Q [8] were fortunately obtained from hydrochloride acid solution by slow evaporation in the presence of CdCl 2 . The complex crystallized in the triclinic crystal system, space group P-1, the compounds consists of one Q [8] host and two Nap1 guests. As shown in Figure 6a, X-ray structural analysis revealed that the naphthyl moiety of the guest Nap1 adopted reverse parallel in the Q [8] cavity, the bipyridyl moiety remained outside of its portal, which could be attributed to π···π interactions in the cavity of Q [8]. Outside of the inclusion complexes, neighboring Nap1 molecules contacted with each other through not only π···π interaction, but also C-H···π interactions (Figure 6b), which may serve to stabilize this structure for building a one-dimensional supramolecular chain of the complexes. In the latter, the encapsulated guests were electron donor and acceptor pair, and the major driving force for the ternary complex formation appears to be strong charge-transfer interaction between the guests [45]. Furthermore, the strong hydrogen-bonding played a critical role in the formation of this host-guest inclusion complex, e.g., between H on methylene of Nap1 and the carbonyl oxygens at the portals of the Q [8] host C(59)-H···O (6)

Materials and Methods
All reagents were obtained from commercial suppliers and used without further purification unless otherwise noted. All reactions were carried out under a dry nitrogen atmosphere. All solvents were dried before use following standard procedures. 1 H and 13 C NMR spectra were recorded on 400 MHz spectrometers in the indicated solvents at room temperature (298 K). Dynamic light scattering (DLS) measurement was conducted on Malvern Zetasizer Nano ZS90 using a monochromatic coherent He-Ne laser (633 nm) as the light source and a detector that detected the scattered light at an angle of 90°. An isothermal titration calorimetry (ITC) experiment was carried out using a MicroCal PEAQ-ITC (Malven Panalytical, Worcestershire, UK) instrument. Association constants and associated thermodynamic parameters were obtained through computer simulations (curve fitting) using Micro-Cal ITC analyze software (MicroCal Origin 4.1). UV-vis spectra were detected on a PerkinElmer 750s instrument from 200 to 800 nm at the scan rate of 3 nm/internal.
Single crystals of NapA2@Q [8] and Nap12@Q [8] were grown from hydrochloride acid solution by slow evaporation. The crystal culture conditions showed that the self-assembly NapA2@Q [8] and Nap12@Q [8] had good stability in strong acid solution. Diffraction data of both complexes were collected at 273(2) K with a Bruker SMART Apex-II CCD diffractometer using graphite-monochromated Mo-Kα radiation (λ = 0.71073 Å). Empirical absorption corrections were performed by using the multi-scan program SADABS. Structural solution and full-matrix least-squares refinement based on F2 were performed with the SHELXS-97 and SHELXL-97 program packages, respectively. Non-hydrogen atoms were treated anisotropically in all cases. All hydrogen atoms were introduced as riding atoms with an isotropic displacement parameter equal to 1.2 times that of the parent atom. Hydrogen atoms were given for all isolated water molecules.
CCDC 2223335 (Nap12@Q [8]) and 2223329 (NapA2@Q [8]) contain the supplementary crystallographic data for this paper. These data can be obtained free of charge from The Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/data_request/cif, which accessed on 3 December 2022.

Materials and Methods
All reagents were obtained from commercial suppliers and used without further purification unless otherwise noted. All reactions were carried out under a dry nitrogen atmosphere. All solvents were dried before use following standard procedures. 1 H and 13 C NMR spectra were recorded on 400 MHz spectrometers in the indicated solvents at room temperature (298 K). Dynamic light scattering (DLS) measurement was conducted on Malvern Zetasizer Nano ZS90 using a monochromatic coherent He-Ne laser (633 nm) as the light source and a detector that detected the scattered light at an angle of 90 • . An isothermal titration calorimetry (ITC) experiment was carried out using a MicroCal PEAQ-ITC (Malven Panalytical, Worcestershire, UK) instrument. Association constants and associated thermodynamic parameters were obtained through computer simulations (curve fitting) using Micro-Cal ITC analyze software (MicroCal Origin 4.1). UV-vis spectra were detected on a PerkinElmer 750s instrument from 200 to 800 nm at the scan rate of 3 nm/internal.
Single crystals of NapA 2 @Q [8] and Nap1 2 @Q [8] were grown from hydrochloride acid solution by slow evaporation. The crystal culture conditions showed that the self-assembly NapA 2 @Q [8] and Nap1 2 @Q [8] had good stability in strong acid solution. Diffraction data of both complexes were collected at 273(2) K with a Bruker SMART Apex-II CCD diffractometer using graphite-monochromated Mo-Kα radiation (λ = 0.71073 Å). Empirical absorption corrections were performed by using the multi-scan program SADABS. Structural solution and full-matrix least-squares refinement based on F2 were performed with the SHELXS-97 and SHELXL-97 program packages, respectively. Non-hydrogen atoms were treated anisotropically in all cases. All hydrogen atoms were introduced as riding atoms with an isotropic displacement parameter equal to 1.2 times that of the parent atom. Hydrogen atoms were given for all isolated water molecules.
CCDC 2223335 (Nap1 2 @Q [8]) and 2223329 (NapA 2 @Q [8]) contain the supplementary crystallographic data for this paper. These data can be obtained free of charge from The Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/data_request/cif, which accessed on 3 December 2022.

Conclusions
In summary, we investigated the host-guest complexation of Q [8] with two enantiomers, NapA and Nap1, in both aqueous solution and solid state by using NMR, UV-vis spectrum, ITC, DLS, and single-crystal X-ray crystallography. Driven by the cooperativity of electrostatic interactions, multiple C-H···π interactions, and hydrogen-bonding, both NapA and Nap1 can be encapsulated into the cavity of Q [8] to form stable homoternary complexes NapA 2 @Q [8] and Nap1 2 @Q [8]. Structure analysis shows that hydrogen-bonding interactions and π···π interactions play a critical role not only in the formation of 1D extended chains, but also in the construction of 2D and 3D networks. This study shows that Q [8] host and molecules containing naphthalene units can be used as building units to build a diverse supramolecular network structure.  Table S1. ITC measurements of the thermodynamics of NapA 2 @Q [8] and