Novel Pyridinium Based Ionic Liquid Promoter for Aqueous Knoevenagel Condensation: Green and Efficient Synthesis of New Derivatives with Their Anticancer Evaluation

Herein, a distinctive dihydroxy ionic liquid ([Py-2OH]OAc) was straightforwardly assembled from the sonication of pyridine with 2-chloropropane-1,3-diol by employing sodium acetate as an ion exchanger. The efficiency of the ([Py-2OH]OAc as a promoter for the sono-synthesis of a novel library of condensed products through DABCO-catalyzed Knoevenagel condensation process of adequate active cyclic methylenes and ninhydrin was next investigated using ultimate greener conditions. All of the reactions studied went cleanly and smoothly, and the resulting Knoevenagel condensation compounds were recovered in high yields without detecting the aldol intermediates in the end products. Compared to traditional strategies, the suggested approach has numerous advantages including mild reaction conditions with no by-products, eco-friendly solvent, outstanding performance in many green metrics, and usability in gram-scale synthesis. The reusability of the ionic liquid was also studied, with an overall retrieved yield of around 97% for seven consecutive runs without any substantial reduction in the performance. The novel obtained compounds were further assessed for their in vitro antitumor potential toward three human tumor cell lines: Colo-205 (colon cancer), MCF-7 (breast cancer), and A549 (lung cancer) by employing the MTT assay, and the findings were evaluated with the reference Doxorubicin. The results demonstrated that the majority of the developed products had potent activities at very low doses. Compounds comprising rhodanine (5) or chromane (12) moieties exhibited the most promising cytotoxic effects toward three cell lines, particularly rhodanine carboxylic acid derivative (5c), showing superior cytotoxic effects against the investigated cell lines compared to the reference drug. Furthermore, automated docking simulation studies were also performed to support the results obtained.

Molecules 2022, 27, x FOR PEER REVIEW 3 decade, developing potential research and technology [40]. They have essential rol synthesis, catalysis, extraction, electrochemistry, analytics, biotechnology, materia ence, and other domains due to their highly tunable nature and extraordinary prope Moreover, depending on the application, these properties might be adjusted by var the cation and anion combinations [41]. Interestingly, ionic liquids are separated in ous phases that reduce the use of combustible, volatile, and poisonous solvents and itate the work-up processes and avoid time-consuming purification procedures [4 45]. The base-catalyzed addition to a carbonyl motif in the Knoevenagel reaction is ated by a nucleophilic attack accompanied by a protonation process [46]. On the other acid-catalyzed by the Knoevenagel reaction would cause the protonation of a carb group that precedes via the attack of a weak nucleophile [26]. Therefore, introducin ionic liquid with hydroxyl groups might cause an acceleration of the reaction path [47,48]. In pursuit of our works on the synthesis of novel ILs and their utilization as lysts and/or solvents in diverse chemical conversions [43,48,49], we designed and pared a new ionic liquid ([Py-2OH]OAc) with two hydroxyl groups. Under ultra cation, the performance of novel IL was established for DABCO-catalyzed Knoeven reactions. The automated docking simulation studies were investigated to support th sults obtained. The newly synthesized compounds were also screened for antiproli tive activity.  [2,4,7,9,10,12,14,15]. The carcinogenic activities of rhodanines have yet to be thoroughly addressed. Aldose reductase (AR) has lately been reported to be an essential regulator of signaling pathways comprising oxidative stress-induced inflammatory diseases, notably cardiovascular diseases, cancers, sepsis, and asthma [17]. AR accumulation in inflammatory-mediated breast, liver, and colon tumors is increasing its potential as a targeted therapy [18]. Moreover, several reports have demonstrated that AR is essential in the drugs' resistance to numerous carcinogenic medicines such as cisplatin, daunorubicin, and others through increasing metabolism, increasing efflux, or diminished penetration of medications [19]. As a consequence, inhibiting AR with medical therapy or genetic modification might be a unique strategy for treating drug-resistant tumors.
On binding to synthetic or natural ligands, peroxisome proliferator-activated receptors (PPARs) have displayed transcription mediators that control gene production and inhibition [20]. PPARs are involved in differential tissue distribution and mediate specific functions in early development, cell proliferation, differentiation, apoptosis, and metabolic homeostasis [20]. PPARγ and their ligands have vital roles in regulating both apoptotic pathways. PPARγ agonists (e.g., TZDs) have been shown to induce apoptosis in various cancer cells including lymphoma, multiple myeloma, bladder, gastric, esophageal, pancreatic, hepatoma, colon, breast, brain, and lung cancer cells [21]. Various works have shown that combining PPARγ agonists with anticancer agents can further sensitize tumor cells to apoptosis. PPARγ plays inhibitory roles in cell growth and proliferation by favoring cell differentiation [22]. Moreover, PPARγ, activated by natural and synthetic ligands, is a promising option for preventing and treating cancer. Knoevenagel condensation is a substantial reaction for generating carbon-carbon bonds via the interaction between active methylene and carbonyl derivatives [23,24]. The α,β-unsaturated carbonyl molecules created by the Knoevenagel reaction could be utilized to design essential precursors for a variety of high-value substances including pharmaceuticals, cosmetics, natural products, fine chemicals, and multifunction polymers [25,26]. Consequently, study on such reactions seems to have been a hot issue in organic syntheses, and sustainable protocols are necessitated. Recently, diverse catalytic approaches have been established for the Knoevenagel reaction employing some designing catalysts such as zeolites [27], polymers [28], functionalized fibers [29], graphene oxide [30], complexes [31], enzymes [32], nanoparticles [33], MOFs [34], and ionic liquids [35,36] have indicated wide applications. Catalysts have shown productivity of the reactions and potentially influence their selectivity [37][38][39]. Ionic liquids (ILs) have had a wide and cutting-edge influence during the last decade, developing potential research and technology [40]. They have essential roles in synthesis, catalysis, extraction, electrochemistry, analytics, biotechnology, material science, and other domains due to their highly tunable nature and extraordinary properties. Moreover, depending on the application, these properties might be adjusted by varying the cation and anion combinations [41]. Interestingly, ionic liquids are separated in various phases that reduce the use of combustible, volatile, and poisonous solvents and facilitate the work-up processes and avoid time-consuming purification procedures [40,[42][43][44][45]. The base-catalyzed addition to a carbonyl motif in the Knoevenagel reaction is initiated by a nucleophilic attack accompanied by a protonation process [46]. On the other site, acid-catalyzed by the Knoevenagel reaction would cause the protonation of a carbonyl group that precedes via the attack of a weak nucleophile [26]. Therefore, introducing an ionic liquid with hydroxyl groups might cause an acceleration of the reaction pathway [47,48]. In pursuit of our works on the synthesis of novel ILs and their utilization as catalysts and/or solvents in diverse chemical conversions [43,48,49], we designed and prepared a new ionic liquid ([Py-2OH]OAc) with two hydroxyl groups. Under ultrasonication, the performance of novel IL was established for DABCO-catalyzed Knoevenagel reactions. The automated docking simulation studies were investigated to support the results obtained. The newly synthesized compounds were also screened for antiproliferative activity.

Experimental
The melting points were determined employing the Electrothermal IA9100 melting point instrument (UK), and the findings were given uncorrected. The IR spectra (KBr) from a Perkin-Elmer Spectrum One spectrometer were recorded. The NMR spectra were obtained by employing a Bruker AV instrument with 400 MHz. A Bruker Daltonics microTOF spectrometer was used to obtain high-resolution mass spectra (HRMS). The purity of substances was determined using analytical thin-layer chromatography (TLC) on an EM silica gel F254 sheet (0.2 mm). The sonication reactions were carried out in an SY5200DH-T ultrasound cleaner.
In a closed vial, a mixture of pyridine (0.85 mL, 1.0 mmol) and 2-chloropropane-1,3diol (0.11 g, 1.0 mmol) was sonicated at 70 • C for 3 h. The obtained crude product was purified by recrystallization from acetonitrile to produce the pure product (99%) as white crystals (1). Then, to the residue of 1 in EtOH (15.0 mL), NaOAc (0.082 g, 1.0 mmol) was introduced. The resulting solution was sonicated at 70 • C for 30 min, following which the solvent was removed under reduced pressure to yield the corresponding IL (2)  To a mixture of appropriate active methylene (1.0 mmol) in IL-H 2 O-DABCO (60-40%-1.0 mmol), 1.0 mmol of carbonyl compounds (3, 13, and 15) was added. The resulting solution was sonicated at 30 • C for 5 min (TLC). After cooling to room temperature, the obtained crystals were filtered off, dried, and crystallized from a proper solvent. zolium bromide) analyses were employed to evaluate the compounds' cytotoxic effects. In 96-well plates, cells (1 × 104/well) were plated in 100.0 mL of medium/well. After overnight incubation, the cells were handled with various drug doses in RPMI 1640 with 10% FBS for 24 h. MTT was then introduced, and the cells were maintained for four hours. MTT-formazan was prepared in 150.0 mL DMSO and stirred for 10 min. At a test wavelength of 490 nm, the absorbance was determined using an ELISA reader. Every experiment was carried out three times in total. The IC 50 is the concentration of the substance that delivers a 50% growth inhibitory value.

Protocol of Docking Studies
Automated docking simulation studies were performed using Molecular Operating Environment (MOE ® ) version 2014.09. The X-ray crystallographic structures of the target AKR1B10 and PPARγ from the protein data bank (PDB: 4JIH, 7E0A) were obtained from the protein data bank. The target compounds were constructed into a 3D model using the builder interface of the MOE ® program after checking their structures and the formal charges on atoms by 2D depiction.

Results and Discussion
Lately, water has been utilized to promote organic reactions as an eco-sustainable solvent [35,50]. The homogeneous solution comprised of water and ionic liquid could exhibit superior characteristics to pure water or ionic ILs, encouraging their utilization in organic synthesis. Accordingly, the IL comprising two hydroxyl groups, 1-(1,3-dihydroxypropan-2yl)pyridin-1-ium chloride ([Py-2OH]Cl), was effectively delivered via the reaction displayed in Scheme 1. The quaternization of 2-chloropropane-1,3-diol with pyridine (Scheme 1) occurred under ultrasonic irradiation conditions (70 • C). Notwithstanding, the reaction afforded a poor yield on performing the reaction in an opened vessel. In contrast, the reaction yield was improved by employing the reaction in a closed vial. To study the temperature impact, this reaction (Scheme 1) was repeated at three different temperatures viz 50, 70, and 90 • C, and the optimal conversion (99% in 3 h) was observed at 70 • C (at 90 • C, neither the reaction yield nor the rate was enhanced). In other respects, water-soluble ILs comprising chloride ions are potentially liberated HCl in water due to hydrolysis [51,52], presenting a significant hazard of water pollution. In the perspective of environmental issues and ILs solubility, the halogen-free IL ([Py-2OH]OAc, 2) was assembled through the reaction of IL (1) with sodium acetate as the anion exchanger (Scheme 1). The molecular structure of the synthesized IL (2) was achieved by HRMS and NMR spectroscopies (See Supplementary Materials). The designed ionic liquid ([Py-2OH]OAc, 2) was employed as a promoter in the DABCO-catalyzed Knoevenagel reactions between various cyclic active methylene derivatives and ninhydrin (Schemes 2-6).

MTT Assay for Cell Viability/Proliferation
Cell lines: ATCC provided the investigated cells; Colo-205 (colon cancer), MCF-7 (breast cancer), and A549 (lung cancer) via Holding firm for bio-supplies and vaccinations (VACSERA) in Cairo, Egypt. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) analyses were employed to evaluate the compounds' cytotoxic effects. In 96-well plates, cells (1 × 104/well) were plated in 100.0 mL of medium/well. After overnight incubation, the cells were handled with various drug doses in RPMI 1640 with 10% FBS for 24 h. MTT was then introduced, and the cells were maintained for four hours. MTT-formazan was prepared in 150.0 mL DMSO and stirred for 10 min. At a test wavelength of 490 nm, the absorbance was determined using an ELISA reader. Every experiment was carried out three times in total. The IC50 is the concentration of the substance that delivers a 50% growth inhibitory value.

Protocol of Docking Studies
Automated docking simulation studies were performed using Molecular Operating Environment (MOE ® ) version 2014.09. The X-ray crystallographic structures of the target AKR1B10 and PPAR from the protein data bank (PDB: 4JIH, 7E0A) were obtained from the protein data bank. The target compounds were constructed into a 3D model using the builder interface of the MOE ® program after checking their structures and the formal charges on atoms by 2D depiction.

Results and Discussion
Lately, water has been utilized to promote organic reactions as an eco-sustainable solvent [35,50]. The homogeneous solution comprised of water and ionic liquid could exhibit superior characteristics to pure water or ionic ILs, encouraging their utilization in organic synthesis. Accordingly, the IL comprising two hydroxyl groups, 1-(1,3-dihydroxypropan-2-yl)pyridin-1-ium chloride ([Py-2OH]Cl), was effectively delivered via the reaction displayed in Scheme 1. The quaternization of 2-chloropropane-1,3-diol with pyridine (Scheme 1) occurred under ultrasonic irradiation conditions (70 °C ). Notwithstanding, the reaction afforded a poor yield on performing the reaction in an opened vessel. In contrast, the reaction yield was improved by employing the reaction in a closed vial. To study the temperature impact, this reaction (Scheme 1) was repeated at three different temperatures viz 50, 70, and 90 °C, and the optimal conversion (99% in 3 h) was observed at 70 °C (at 90 °C , neither the reaction yield nor the rate was enhanced). In other respects, water-soluble ILs comprising chloride ions are potentially liberated HCl in water due to hydrolysis [51,52], presenting a significant hazard of water pollution. In the perspective of environmental issues and ILs solubility, the halogen-free IL ([Py-2OH]OAc, 2) was assembled through the reaction of IL (1) with sodium acetate as the anion exchanger (Scheme 1). The molecular structure of the synthesized IL (2) was achieved by HRMS and NMR spectroscopies (See SI). The designed ionic liquid ([Py-2OH]OAc, 2) was employed as a promoter in the DABCO-catalyzed Knoevenagel reactions between various cyclic active methylene derivatives and ninhydrin (Schemes 2-6). Molecules 2022, 27, x FOR PEER REVIEW 7 To commence, we selected the widely available ninhydrin (3, 1.0 mmol) an methylrhodanine (4a, 1.0 mmol) as model reactants to evaluate the efficiency of the c structed ionic liquid [Py-2OH]OAc (Scheme 2).
To evaluate the model reaction (Scheme 2) from the green chemistry standpoint, ultrasound conditions were coupled under regimes of ionic liquid. After that, diversi parameters were extensively studied to achieve the optimal conditions including ene source, reaction duration, temperature, bases, IL loading, and solvents. Through the ication (30 °C) of the template reactants in IL-H2O (1:1 equiv), a moderate yield of hitherto unreported 2-(3-methyl-4-oxo-2-thioxothiazolidin-5-ylidene)-1H-indene-1,3(2 dione (5a) was obtained ( Table 1, entry 1), along with traces of the aldol derivative HRMS; m/z: 306.9972, corresponding to C13H9NO4S2) while the targeted product (5a) not detected in the reaction profile (TLC) when performing the reaction in an aque medium (Table 1, entry 2). Interestingly, once the model reaction was carried out utiliz IL-H2O-DABCO (3 g:3 mL:1.0 mmol), the reaction profile was quite clear, and Knoevenagel product (5a) was generated as the sole product (Scheme 2) without detec the aldol product (6) ( Table 1, entry 3). When organic bases including piperidine, h methylenetetramine (HMTA), or triethylamine (TEA) were employed rather t DABCO, moderate (75%) to good (83%) yields were achieved (Table 1, entries 4-6). sides, a moderate yield was detected by employing the inorganic weak base K2CO3 (T 1, entry 7). Unfortunately, when KOH was introduced, only 56% yield was achieved ble 1, entry 8) with an unclear reaction profile. Furthermore, reducing the DABCO amo to 0.5 equiv diminished the productivity while increasing its amount to 2.0 equiv, so b the reaction yield and rate were unaffected. To further emphasize that the improvem resulted from the IL-H2O-DABCO system, IL-H2O and H2O-DABCO were also ployed ( Table 1, entries 1 and 9). The results displayed that, in both cases, the yields w diminished. Consequently, DABCO will be performed as the best basic candidate in subsequent studies. Various solvents ( Table 1, entries [10][11][12] were also screened, and ter scored a superior yield within a short reaction time. This finding suggests that current reaction (Scheme 2) belongs to the "on water" category and is therefore envi mentally-friendly [53]. The reaction productivity was reduced when the model reac (Scheme 2) was carried out at room temperature (23 °C) ( Table 1, entry 13). In addit increasing the temperature from 30 to 40 °C yielded the derivative 5a in almost sim yield (88%) albeit with a prolonged time (Table 1, entry 14). Both reaction producti and rate were not enhanced by gradually increasing the reaction temperature from 6 Molecules 2022, 27, x FOR PEER REVIEW 9 of 21 Scheme 3. Ultrasound-assisted protocol for the preparation of 5a-d.

Scheme 4.
Ultrasound-assisted protocol for the preparation of 8a-d.
Switching to pyrazolone and oxazolone derivatives (9a,b), the reactions also proceeded effectively and provided the targeted products with excellent yields (Scheme 5). In addition, the reaction smoothly proceeded with imidazolidinone derivatives (7ad) and afforded Knoevenagel condensation products (8a-d) in almost quantitative yields (Scheme 4). Switching to pyrazolone and oxazolone derivatives (9a,b), the reactions also proceeded effectively and provided the targeted products with excellent yields (Scheme 5).  Switching to pyrazolone and oxazolone derivatives (9a,b), the reactions also proceeded effectively and provided the targeted products with excellent yields (Scheme 5).

Scheme 5.
Ultrasound-assisted protocol for the preparation of 10a,b.
Interestingly, the present method proved to be precisely as efficacious as in the instance of ninhydrin, and the related products were efficiently synthesized in high yields (Tables 2 and 3). Tables 2 and 3 demonstrate a comparison of the present approach with the reported procedures for the preparation of 14a-d and 16a,b to show the merits and efficiency of the present work. In the perspective of reaction time, yield, and yield economy, utilizing [Py-2OH]AcO under ultrasound conditions is undoubtedly the optimum alternative for such a Knoevenagel reaction.
In an attempt to assess the feasibility of scaling up the aforesaid procedure, the template reaction (Scheme 7) was carried out on a gram scale employing the optimal conditions that in turn yielded the required product (5a) in superior yield (98%; Scheme 7). Moreover, to assess the sustainability of the established procedure, various environmental metrics [26,58] such as atom economy (AE), yield economy (YE), environmental factor (EF), carbon efficiency (CE), reaction mass efficiency (RME), and process mass intensity (PMI) were also addressed (Scheme 7). As displayed in Scheme 7, the reaction scored the required criteria (lower values of EF and PMI, and higher values of RME, AE, CE, and YE). Therefore, it might be considered as a sustainable and eco-friendly procedure. To commence, we selected the widely available ninhydrin (3, 1.0 mmol) and 3methylrhodanine (4a, 1.0 mmol) as model reactants to evaluate the efficiency of the constructed ionic liquid [Py-2OH]OAc (Scheme 2).
To evaluate the model reaction (Scheme 2) from the green chemistry standpoint, the ultrasound conditions were coupled under regimes of ionic liquid. After that, diversified parameters were extensively studied to achieve the optimal conditions including energy source, reaction duration, temperature, bases, IL loading, and solvents. Through the sonication (30 • C) of the template reactants in IL-H 2 O (1:1 equiv), a moderate yield of the hitherto unreported 2-(3-methyl-4-oxo-2-thioxothiazolidin-5-ylidene)-1H-indene-1,3(2H)-dione (5a) was obtained (Table 1, entry 1), along with traces of the aldol derivative (6, HRMS; m/z: 306.9972, corresponding to C 13 H 9 NO 4 S 2 ) while the targeted product (5a) was not detected in the reaction profile (TLC) when performing the reaction in an aqueous medium (Table 1, entry 2). Interestingly, once the model reaction was carried out utilizing IL-H 2 O-DABCO (3 g:3 mL:1.0 mmol), the reaction profile was quite clear, and the Knoevenagel product (5a) was generated as the sole product (Scheme 2) without detecting the aldol product (6) ( Table 1, entry 3). When organic bases including piperidine, hexamethylenetetramine (HMTA), or triethylamine (TEA) were employed rather than DABCO, moderate (75%) to good (83%) yields were achieved (  entries 1 and 9). The results displayed that, in both cases, the yields were diminished. Consequently, DABCO will be performed as the best basic candidate in the subsequent studies. Various solvents ( Table 1, entries 10-12) were also screened, and water scored a superior yield within a short reaction time. This finding suggests that the current reaction (Scheme 2) belongs to the "on water" category and is therefore environmentally-friendly [53]. The reaction productivity was reduced when the model reaction (Scheme 2) was carried out at room temperature (23 • C) ( Table 1, entry 13). In addition, increasing the temperature from 30 to 40 • C yielded the derivative 5a in almost similar yield (88%) albeit with a prolonged time (Table 1,  entry 14). Both reaction productivity and rate were not enhanced by gradually increasing the reaction temperature from 60 to 80 • C (Table 1, entries 15 and 16), thus establishing 30 • C is the optimal temperature. Furthermore, the proportion of IL (2) utilized in the process (Scheme 2) was investigated concerning the water content. An excellent yield (99%) was obtained in 5 min when the used proportion of IL:H 2 O was 60:40% (Table 1, entry 17). Decreasing the percentage of IL slightly diminished the yield, and the time became considerably longer (Table 1, entry 18). This might be because using a high percentage of water reduces the reactants' dissolution, a fact confirmed by observing a considerable amount of insoluble reactants in the reaction medium whereas increasing the percentage of IL by more than 60% did not improve the reaction rate or yield (Table 1, entry 19). Besides, employing the model reaction in less than 5 min diminished the yield. Furthermore, performing the model reaction under conventional conditions resulted in diminishing the reaction yield (Table 1, entry 20). According to the above studies (Table 1) Table 1, entry 17). The noticeable efficiency of the IL might be attributed to the presence of two hydroxyl groups that acted as a protic additive in promoting the reaction. After effectively developing an optimal approach for the template reaction, we moved on to investigate the reaction's scope and the variety of the substrates. Thus, in the next study, a varied array of functionalized cyclic active methylenes (4, 7, 9, and 11) underwent rapid Knoevenagel reactions with ninhydrin (3) to produce hitherto unknown compounds (5, 8, 10, and 12) in nearly quantitative yields (up to 99%, Schemes 3-6).
Generally, rhodanines such as 4a-d provided marginally higher yields of the respective products than the remaining active cyclic methylenes.
Within the rhodanine series, it was found that the electronic structure of the substituents has a minimal influence on the reaction performance. Both electron-releasing motifs (N-methyl and N-ethyl) and electron-attracting motifs (N-allyl and N-acetic acid) were amenable to the reaction (Scheme 3).
Switching to pyrazolone and oxazolone derivatives (9a,b), the reactions also proceeded effectively and provided the targeted products with excellent yields (Scheme 5).
Having synthesizing targeted Knoevenagel products efficiently, we next evaluated the scalability of the conventional approach by substituting ninhydrin with other synthons such as isatin (   Having synthesizing targeted Knoevenagel products efficiently, we next evaluated the scalability of the conventional approach by substituting ninhydrin with other synthons such as isatin (Table 2) [54][55][56] and acenaphthenequinone (Table 3) [55,57].
Interestingly, the present method proved to be precisely as efficacious as in the instance of ninhydrin, and the related products were efficiently synthesized in high yields (Tables 2 and 3). Tables 2 and 3 demonstrate a comparison of the present approach with the reported procedures for the preparation of 14a-d and 16a,b to show the merits and efficiency of the present work. In the perspective of reaction time, yield, and yield economy, utilizing [Py-2OH]AcO under ultrasound conditions is undoubtedly the optimum alternative for such a Knoevenagel reaction.
In an attempt to assess the feasibility of scaling up the aforesaid procedure, the template reaction (Scheme 7) was carried out on a gram scale employing the optimal conditions that in turn yielded the required product (5a) in superior yield (98%; Scheme 7). Moreover, to assess the sustainability of the established procedure, various environmental metrics [26,58] such as atom economy (AE), yield economy (YE), environmental factor (EF), carbon efficiency (CE), reaction mass efficiency (RME), and process mass intensity (PMI) were also addressed (Scheme 7). As displayed in Scheme 7, the reaction scored the required criteria (lower values of EF and PMI, and higher values of RME, AE, CE, and YE). Therefore, it might be considered as a sustainable and eco-friendly procedure.   Furthermore, to evaluate the feasibility of IL (2) reuse, the synthesis of 5a was selected for this assignment. The reusability of the IL (2) was studied by repeating the model reaction under optimal conditions (Scheme 2). The obtained product was separated through simple filtration following every run, and the IL was retrieved by removing water under vacuum and reused in the next run. The IL was evidenced to be highly stable ( 1 H NMR after the seventh run was the same as the original one) at optimum conditions and could be recycled up to seven cycles without a considerable loss of potency ( Figure 2). Interestingly, the present method proved to be precisely as efficacious as in the instance of ninhydrin, and the related products were efficiently synthesized in high yields (Tables 2 and 3). Tables 2 and 3 demonstrate a comparison of the present approach with the reported procedures for the preparation of 14a-d and 16a,b to show the merits and efficiency of the present work. In the perspective of reaction time, yield, and yield economy, utilizing [Py-2OH]AcO under ultrasound conditions is undoubtedly the optimum alternative for such a Knoevenagel reaction.
In an attempt to assess the feasibility of scaling up the aforesaid procedure, the template reaction (Scheme 7) was carried out on a gram scale employing the optimal conditions that in turn yielded the required product (5a) in superior yield (98%; Scheme 7). Moreover, to assess the sustainability of the established procedure, various environmental metrics [26,58] such as atom economy (AE), yield economy (YE), environmental factor (EF), carbon efficiency (CE), reaction mass efficiency (RME), and process mass intensity (PMI) were also addressed (Scheme 7). As displayed in Scheme 7, the reaction scored the required criteria (lower values of EF and PMI, and higher values of RME, AE, CE, and YE). Therefore, it might be considered as a sustainable and eco-friendly procedure.
Furthermore, to evaluate the feasibility of IL (2) reuse, the synthesis of 5a was selected for this assignment. The reusability of the IL (2) was studied by repeating the model reaction under optimal conditions (Scheme 2). The obtained product was separated through simple filtration following every run, and the IL was retrieved by removing water under vacuum and reused in the next run. The IL was evidenced to be highly stable ( 1 H NMR after the seventh run was the same as the original one) at optimum conditions and could be recycled up to seven cycles without a considerable loss of potency ( Figure 2). Furthermore, to evaluate the feasibility of IL (2) reuse, the synthesis of 5a was selected for this assignment. The reusability of the IL (2) was studied by repeating the model reaction under optimal conditions (Scheme 2). The obtained product was separated through simple filtration following every run, and the IL was retrieved by removing water under vacuum and reused in the next run. The IL was evidenced to be highly stable ( 1 H NMR after the seventh run was the same as the original one) at optimum conditions and could be recycled up to seven cycles without a considerable loss of potency ( Figure 2). According to the obtained results, a suggested mechanism for this condensation r action has been suggested (Scheme 8). At first, a carbanion was formed due to abstractin the acidic proton from the active methylene motif by DABCO. Simultaneously, the pola ity of the carbonyl group was enhanced via hydrogen bond formation with the two-h droxyl groups of the IL. Second, the generated carbanion underwent a nucleophilic attac at the protonated carbonyl motif, resulting in the adduct A. Furthermore, the IL accele ated the reaction through the proton transfer from the active methylene to the alkoxid (B), resulting in intermediate C. Finally, DABCO-quaternary ammonium proton faci tated the removal of a water molecule and the generation of the olefinic double bon (Scheme 8). According to the obtained results, a suggested mechanism for this condensation reaction has been suggested (Scheme 8). At first, a carbanion was formed due to abstracting the acidic proton from the active methylene motif by DABCO. Simultaneously, the polarity of the carbonyl group was enhanced via hydrogen bond formation with the two-hydroxyl groups of the IL. Second, the generated carbanion underwent a nucleophilic attack at the protonated carbonyl motif, resulting in the adduct A. Furthermore, the IL accelerated the reaction through the proton transfer from the active methylene to the alkoxide (B), resulting in intermediate C. Finally, DABCO-quaternary ammonium proton facilitated the removal of a water molecule and the generation of the olefinic double bond (Scheme 8).

Cytotoxic Activity
Utilizing the MTT test [59], the newly assembled Knoevenagel compounds (5a-d,  8a-d, 10a,b, and 12a,b) were evaluated for their in vitro antitumor effects against an array of human cancer cell lines, including Colo-205 (colon cancer), MCF-7 (breast cancer), and A549 (lung cancer). Table 4 and Figure 3 display the IC 50 (compound concentration that causes a 50% reduction in cell proliferation) measurements for the produced compounds employing Doxorubicin as a reference drug. The antitumor activity results suggested that the majority of the investigated derivatives demonstrated moderate to excellent potency, with IC 50 values ranging from 0.04 to 11.81 µM. Derivatives 5, 8, and 12 demonstrated the most antiproliferative potential toward the three cell lines (IC 50 : 0.04-5.51 M). Of these, the efficiency of compound 5c was approximately 3-fold more than that of Doxorubicin. Further, derivatives 10a and 10b were especially effective at inhibiting Colo-205 cell proliferation.
According to the obtained results, a suggested mechanism for this condensation re action has been suggested (Scheme 8). At first, a carbanion was formed due to abstractin the acidic proton from the active methylene motif by DABCO. Simultaneously, the polar ity of the carbonyl group was enhanced via hydrogen bond formation with the two-hy droxyl groups of the IL. Second, the generated carbanion underwent a nucleophilic attac at the protonated carbonyl motif, resulting in the adduct A. Furthermore, the IL acceler ated the reaction through the proton transfer from the active methylene to the alkoxid (B), resulting in intermediate C. Finally, DABCO-quaternary ammonium proton facil tated the removal of a water molecule and the generation of the olefinic double bond (Scheme 8).

Scheme 8.
A possible reaction mechanism. Scheme 8. A possible reaction mechanism.

Structure-Activity Relationship (SARs)
According to the molecular structures of the newly assembled compounds, derivatives 5-12 could be categorized into four classes: A (5a-d), B (8a-d), C (10a,b), and D (12a,b). Regarding series A, derivative 5c with an acetic acid motif was observed to be the most potent molecule of all series against the three investigated cells (IC50 varied between 0.04 and 0.06 μM) and demonstrated superior in vitro inhibition effects than Doxorubicin. Mechanistically, such carboxylic acids suppressed tube-forming activity induced by vascular endothelial growth factor (VEGF) and thus reduced the tumor growth [60]. Furthermore, compound 5d exhibited similar antitumor activity to the reference drug (IC50 varied between 0.15 and 0.21 μM) against the three examined cell lines, confirming the beneficial effect of the allyl group in the treatment of cancer [61]. The introduction of alkyl motifs (methyl, 5a or ethyl, 5b) to the rhodanine nucleus reduced the in vitro inhibitory effects against three cell lines marginally. When the rhodanine motif was replaced with an imidazole nucleus, the resultant series (class B) effectively contributed to the anticancer activity of the assessed strains. In this series, introducing an alkyl substituent such as methyl (8b or 8d) instead of hydrogen atom (8a or 8c) resulted in a slight diminishing in the inhibitory activities while the introduction of the thione motif (8c) resulted in a remarkable improvement in the cytotoxicity, particularly toward Colo-205 with IC50 of 0.89 μM. This might be due to the existence of a polar thiocarbonyl moiety that may enhance their interaction with

Structure-Activity Relationship (SARs)
According to the molecular structures of the newly assembled compounds, derivatives 5-12 could be categorized into four classes: A (5a-d), B (8a-d), C (10a,b), and D (12a,b). Regarding series A, derivative 5c with an acetic acid motif was observed to be the most potent molecule of all series against the three investigated cells (IC 50 varied between 0.04 and 0.06 µM) and demonstrated superior in vitro inhibition effects than Doxorubicin. Mechanistically, such carboxylic acids suppressed tube-forming activity induced by vascular endothelial growth factor (VEGF) and thus reduced the tumor growth [60]. Furthermore, compound 5d exhibited similar antitumor activity to the reference drug (IC 50 varied between 0.15 and 0.21 µM) against the three examined cell lines, confirming the beneficial effect of the allyl group in the treatment of cancer [61]. The introduction of alkyl motifs (methyl, 5a or ethyl, 5b) to the rhodanine nucleus reduced the in vitro inhibitory effects against three cell lines marginally. When the rhodanine motif was replaced with an imidazole nucleus, the resultant series (class B) effectively contributed to the anticancer activity of the assessed strains. In this series, introducing an alkyl substituent such as methyl (8b or 8d) instead of hydrogen atom (8a or 8c) resulted in a slight diminishing in the inhibitory activities while the introduction of the thione motif (8c) resulted in a remarkable improvement in the cytotoxicity, particularly toward Colo-205 with IC 50 of 0.89 µM. This might be due to the existence of a polar thiocarbonyl moiety that may en-hance their interaction with the hydrophilic binding sites [61]. According to the obtained values of IC 50 (Table 4), it could be inferred that rhodanine derivatives (class A) exhibit superior pharmacodynamic impacts than the imidazoles (class B). The pyrazole derivative 10a (class C) showed significantly high activity (for A549 and Colo-205), which was slightly decreased upon being replaced by an isoxazole ring (10b). This might be attributed to the introduction of a more bulky moiety such as the benzene ring (in derivative 10b), leading to an enhancement in the steric hindrance and therefore reducing the interaction to the target [62]. Compounds 12a and 12b (class D) represented a noteworthy exception, demonstrating comparable antiproliferative potency to the reference drug, indicating that the presence of a chromone ring might considerably enhance potency toward the examined strains. In this series, replacing the hydrogen atom (12a) with a substituent such as a fluoro (F) atom (12b) resulted in a significant increase in activities (Table 4 and Figure 3). This result supported the previous study that the presence of a lipophilic fluoro substituent improved the antiproliferative potential [61].

Docking Study
Enzyme-ligand docking was investigated for three prepared derivatives 5c,d, 12b to predict the binding interactions between the novel prepared derivatives and AKR1B10 and PPARγ protein that resulted from the protein data bank (PDB: 4JIH, 7E0A), respectively, by Molecular Operating Environment (MOE ® ) version 2014.09 ( Table 5). The investigated derivatives were successfully docked into the active sites of AKR1B10 and PPARγ protein compared with Epalrestat as a reference compound (Figure 4). the hydrophilic binding sites [61]. According to the obtained values of IC50 (Table 4), it could be inferred that rhodanine derivatives (class A) exhibit superior pharmacodynamic impacts than the imidazoles (class B). The pyrazole derivative 10a (class C) showed significantly high activity (for A549 and Colo-205), which was slightly decreased upon being replaced by an isoxazole ring (10b). This might be attributed to the introduction of a more bulky moiety such as the benzene ring (in derivative 10b), leading to an enhancement in the steric hindrance and therefore reducing the interaction to the target [62]. Compounds 12a and 12b (class D) represented a noteworthy exception, demonstrating comparable antiproliferative potency to the reference drug, indicating that the presence of a chromone ring might considerably enhance potency toward the examined strains. In this series, replacing the hydrogen atom (12a) with a substituent such as a fluoro (F) atom (12b) resulted in a significant increase in activities (Table 4 and Figure 3). This result supported the previous study that the presence of a lipophilic fluoro substituent improved the antiproliferative potential [61].

Docking Study
Enzyme-ligand docking was investigated for three prepared derivatives 5c,d, 12b to predict the binding interactions between the novel prepared derivatives and AKR1B10 and PPAR protein that resulted from the protein data bank (PDB: 4JIH, 7E0A), respectively, by Molecular Operating Environment (MOE ® ) version 2014.09 ( Table 5). The investigated derivatives were successfully docked into the active sites of AKR1B10 and PPAR protein compared with Epalrestat as a reference compound (Figure 4).  On the other hand, compound 5c binding with PPAR shows three hydrogen bonds with SER 342, LYS 265, and HIS 266 ( Figure 5). On the other hand, compound 5c binding with PPARγ shows three hydrogen bonds with SER 342, LYS 265, and HIS 266 ( Figure 5). Compound 5d modeling configuration with AKR1B1 shows hydrogen bonding interaction with ASN 161 and hydrophobic interactions with SER 211 and TYR 210 ( Figure 6). Docking data for compound 5d with PPAR showed that both the carbonyl-oxygen and the sulfur were hydrogen-bonded with SER 342 and LYS 265, respectively, and hydrophobic interaction with ARG 288 (Figure 7).  Compound 5d modeling configuration with AKR1B1 shows hydrogen bonding interaction with ASN 161 and hydrophobic interactions with SER 211 and TYR 210 ( Figure 6). Compound 5d modeling configuration with AKR1B1 shows hydrogen bonding interaction with ASN 161 and hydrophobic interactions with SER 211 and TYR 210 ( Figure 6). Docking data for compound 5d with PPAR showed that both the carbonyl-oxygen and the sulfur were hydrogen-bonded with SER 342 and LYS 265, respectively, and hydrophobic interaction with ARG 288 (Figure 7).  Docking data for compound 5d with PPARγ showed that both the carbonyl-oxygen and the sulfur were hydrogen-bonded with SER 342 and LYS 265, respectively, and hydrophobic interaction with ARG 288 (Figure 7). Docking data for compound 5d with PPAR showed that both the carbonyl-oxygen and the sulfur were hydrogen-bonded with SER 342 and LYS 265, respectively, and hydrophobic interaction with ARG 288 (Figure 7).  On the other hand, 12b modeling with AKR1B10 displayed a hydrogen bond to ASN 161 and hydrophobic interaction with TYR 219 and SER 211 ( Figure 8). On the other hand, 12b modeling with AKR1B10 displayed a hydrogen bond to ASN 161 and hydrophobic interaction with TYR 219 and SER 211 ( Figure 8). In addition, 12b binds to PPAR with hydrogen bonds with LYS 265 and ARG 288 and hydrophobic interaction with SER 342 (Figure 9).  In addition, 12b binds to PPARγ with hydrogen bonds with LYS 265 and ARG 288 and hydrophobic interaction with SER 342 (Figure 9). In addition, 12b binds to PPAR with hydrogen bonds with LYS 265 and ARG 288 and hydrophobic interaction with SER 342 (Figure 9). The modeling for Epalrestat in AKR1B10 illustrates the carbonyl oxygen and two sulfur groups form hydrogen bonds with SER 211, GLN 184, ASP 44, THR 20, and TRP 21 and hydrophobic interaction with TYR 210 (Figure 10).
Moreover, Epalrestat modeling in PPARγ showed that the carbonyl oxygen is hydrogen-bonded to ARG 288 and the hydrophobic interaction with SER 342 and ARG 288 (Figure 11).  Moreover, Epalrestat modeling in PPARγ showed that the carbonyl oxygen is hydrogenbonded to ARG 288 and the hydrophobic interaction with SER 342 and ARG 288 ( Figure 11).

Conclusions
An eco-friendly, promising, and recyclable ionic liquid was synthesized for the first time from pyridine and 2-chloro-1,3-propanediol using sodium acetate as an ion exchanger. Under ultrasonic irradiation, the designed ionic liquid was employed as a promoter for DABCO-catalyzed Knoevenagel reactions in water. Under these conditions, a new series of Knoevenagel products were obtained in excellent yields. This approach offers significant features including gram-scale practicality, superior environmental criteria, simplicity of work-up, and facile recycling of ionic liquid, making it a sustainable and green approach for synthesizing multifunctional Knoevenagel products. The antiproliferative potencies of the newly obtained compounds were evaluated against three tumor cells viz Colo-205, MCF-7, and A549. The results demonstrate that all of the evaluated compounds displayed various activities for the cancer cell lines studied. Furthermore, SAR assessment showed that compounds containing rhodanine (5) or chromane (12) motifs are necessary for improving the cytotoxic activities. Of them, compound 5c was three times more potent than Doxorubicin, indicating that it could serve as a promising and new candidate for further research. The newly synthesized compounds are presently being studied in our lab as potential precursors to design interesting spiro compounds.

Conclusions
An eco-friendly, promising, and recyclable ionic liquid was synthesized for the first time from pyridine and 2-chloro-1,3-propanediol using sodium acetate as an ion exchanger. Under ultrasonic irradiation, the designed ionic liquid was employed as a promoter for DABCO-catalyzed Knoevenagel reactions in water. Under these conditions, a new series of Knoevenagel products were obtained in excellent yields. This approach offers significant features including gram-scale practicality, superior environmental criteria, simplicity of work-up, and facile recycling of ionic liquid, making it a sustainable and green approach for synthesizing multifunctional Knoevenagel products. The antiproliferative potencies of the newly obtained compounds were evaluated against three tumor cells viz Colo-205, MCF-7, and A549. The results demonstrate that all of the evaluated compounds displayed various activities for the cancer cell lines studied. Furthermore, SAR assessment showed that compounds containing rhodanine (5) or chromane (12) motifs are necessary for improving the cytotoxic activities. Of them, compound 5c was three times more potent than Doxorubicin, indicating that it could serve as a promising and new candidate for further research. The newly synthesized compounds are presently being studied in our lab as potential precursors to design interesting spiro compounds.