Phosphorus Fertilizers from Sewage Sludge Ash and Animal Blood as an Example of Biobased Environment-Friendly Agrochemicals: Findings from Field Experiments

Wastes of biological origin from wastewater treatment systems and slaughterhouses contain substantial amounts of phosphorus (P) with high recovery potential and can contribute to alleviating the global P supply problem. This paper presents the performance of fertilizer (AF) and biofertilizer (BF) from sewage sludge ash and animal blood under field conditions. BF is AF incorporated with lyophilized cells of P-solubilizing bacteria, Bacillus megaterium. In the experiments with spring or winter wheat, the biobased fertilizers were compared to commercial P fertilizer, superphosphate (SP). No P fertilization provided an additional reference. Fertilizer effects on wheat productivity and on selected properties of soil were studied. BF showed the same yield-forming efficiency as SP, and under poorer habitat conditions, performed slightly better than AF in increasing yield and soil available P. Biobased fertilizers applied at the P rate up to 35.2 kg ha–1 did not affect the soil pH, did not increase As, Cd, Cr, Ni, and Pb content, and did not alter the abundance of heterotrophic bacteria and fungi in the soil. The findings indicate that biobased fertilizers could at least partially replace conventional P fertilizers. Research into strain selection and the proportion of P-solubilizing microorganisms introduced into fertilizers should be continued.


Introduction
According to current estimates, the number of people in the world is increasing by more than 200,000 individuals every day [1]. If growth tendencies continue, the world population will approach 9 billion by 2037 and reach 10 billion in 2057 [1]. Since agriculture nowadays provides~97.0% of the global food supply [2], it is mainly this sector that will face the dilemma of feeding the world in the coming decades [3]. The required corresponding increase in food production must come from increased crop productivity (higher yields, cropping intensities) [4,5] because the expansion of agricultural land is limited [4]. An additional challenge is to increase agricultural productivity on a sustainable basis, i.e., without impacting environmental security, while conserving and enhancing biotic and abiotic resources [3,6]. Reducing food loss and waste should be an accompanying strategy [7].
Increased crop productivity comes with the need for plants to take up more nutrients, mainly from soil resources. Soil nutrient abundance or nutrient availability often does not meet the nutritional requirements of high production potential crops, and replenishment of nutrients from external sources is necessary [8]. Chemical/mineral fertilizers are fast-acting nutrient carriers that promote plant growth by rapidly increasing soil fertility [9]. of fertilizer products will come into force in the EU to promote the use of fertilizers made from organic or recycled materials [38].
Given the growing problems associated with sewage sludge disposal (growing production [39], emerging new chemical and biological contaminants [40], legal restrictions for agricultural use [41]), thermal sludge conversion is thought one of the most promising methods of sludge management [42]. Raw sewage sludge ash (SSA), while classified as waste [43], is also seen as a potentially valuable source of P for fertilizer production [44,45]. According to common recent estimates, the annual global production of SSA is about 1.7 million tones and is expected to increase in the future [46]. The P content in dry matter of SSA ranges from less than 10% to less than 20% [47], which is comparable to the content of this element in commercial phosphate rock (10.9-16.13% P) [48]. Apart from P, SSA is also a carrier of other macro-and micronutrients [49]. Although the potential of P recovery from SSA is high, the P bioavailability in SSA is low and more than half of the ashes cannot be used as fertilizers due to their high potentially toxic element (PTE) content [44]. In recent years, several new technologies have been developed with the potential to convert SSA into marketable fertilizer products after further treatment [44,45].
Animal blood is considered one of the main by-products of slaughterhouses, which, after being dried and powdered [50], is widely used as blood meal, an environment-friendly fertilizer [51]. Although blood meal is not rich in P (only 0.22% according to [52]), it contains a high proportion of N (about 12% N [52]) in the N-NO 3 form, which is readily available for plants [53], and trace elements [52]. In addition, animal blood is a good binder that can be used in fertilizer production [52].
A new, innovative approach in waste-based fertilizer production is manufacturing these agrochemicals using a microbial solubilization process [54]. Many naturally occurring microbial organisms, including bacteria, fungi, actinomycetes and algae, exhibit P solubilization ability. Among all microorganisms in the soil, phosphorus-solubilizing microorganisms (PSM) can constitute up to 50% [55]. They are mostly associated with the plant rhizosphere. The most powerful PSM are strains from the bacterial genera Pseudomonas, Bacillus, Rhizobium and Enterobacter along with Penicillium and Aspergillus fungi, and Bacillus megaterium is reported as one of the most important strains [55]. PSM solubilize inorganic P compounds via the release of organic and inorganic acids and phosphatase enzyme [56]. In addition to P solubilization, other biological mechanisms involving PSM (e.g., nitrogen fixation, potassium solubilization, phytohormone excretion, the release of antibiotics and antifungal metabolites, guarding plants from abiotic and biotic stresses and pollutant detoxification) function in soil, allowing them to promote plant growth directly or indirectly [57,58]. PSM have already been used in agriculture: alone, to activate 'legacy P' [59], or with P substrate of low plant availability [60]. Any substance containing live microorganisms that exhibit beneficial properties for plant growth and development is named a biofertilizer [61]. Recently, PSM have also been shown to effectively solubilize P from waste materials [62]. These findings provided the basis for the development of a technology to produce biofertilizers from secondary raw materials in a formulation in which living PSM cultures were incorporated [54].
The recommendation of all waste biomass-based agrochemicals as substitutes for conventional fertilizers should be based on the results of their agronomic evaluation conducted under real (field) conditions. This evaluation should include not only a yield-forming efficiency analysis but also agricultural product quality and environmental impact. Although many papers on modern P recovery technologies and recycled fertilizer production have been published in recent years [44,45,[63][64][65][66][67], field test reports of such agrochemicals are not very common [68,69]. This paper presents the major results of the field evaluation of two biomass-based agrochemicals produced from SSA and dried animal blood, i.e., fertilizer (AF) and biofertilizer (BF, i.e., AF containing B. megaterium cells). The aim of the study was to assess the effect of these chemicals on wheat (test crop) productivity, i.e., grain yield and yield structure components, and on selected properties of the soil environment under the test crop, i.e., pH, available P content, the content of PTE (As, Cd, Cr, Ni and Pb) and the abundance of heterotrophic bacteria and fungi. It was hypothesized that the new biomass-based fertilizers would not show inferior yield-forming efficiency versus a commercial P fertilizer, superphosphate (SP), and their impact on the soil environment should be the same or more beneficial than that of SP.

Fertilizer Characteristics
In field conditions, two biomass-based P agrochemicals were applied: a biomass-based fertilizer (AF) and a biomass-based biofertilizer (BF). The raw materials for their production were: ash from the incineration of sewage sludge biomass from wastewater treatment (sewage sludge ash, SSA; Olsztyn, Poland) and dried animal (porcine) blood. The BF was additionally incorporated with lyophilized cells of Bacillus megaterium, a P-solubilizing bacteria strain. The SSA was supplied by the Municipal Wastewater Treatment Plant 'Łyna' in Olsztyn (Olsztyn, Poland). Dried blood was sourced from the meat processing industry. Bacterial lyophilizate was acquired from the Polish Collection of Microorganisms at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław (Wrocław, Poland). Application batches of AF and BF as granules were prepared at the Institute of New Chemical Syntheses in Puławy (Puławy, Poland) [70] following a concept elaborated at the Wrocław University of Science and Technology (Wrocław, Poland) [54]. Table 1 shows the elemental composition of the biobased fertilizers under study.

Experimental Design and Agronomic Management
Three field experiments were conducted with common wheat (Triticum aestivum ssp. vulgare MacKey), spring (SW-2016, SW-2017) or winter (WW-2017; sown in 2016), as a test plant. In each experiment, AF and BF were tested against SP and no P treatments (P 0 ). All P-fertilizers were applied at three different P doses: (1) 17.6, (2) 26.4, and (3) 35.2 kg ha -1 . As a result, ten treatments of P fertilization were compared (Table 2; [71]). P-fertilizer application on experimental plots was performed pre-sowing, simultaneously with potassium (K) fertilizers and the first part of the nitrogen (N) fertilizer dose. The fertilizers were distributed manually on the plot surface and were then mixed with soil by harrowing. Detailed agronomic information is provided in Table S1.
The experiments were arranged in a randomized block design. In each experiment, individual experimental treatments were executed in four replications (plots of 20 m 2 each).

Study Site, Soil and Weather Conditions
The study area was located in north-eastern Poland (Warmińsko-Mazurskie Province), in the fields of the Production and Experimental Station 'Bałcyny' Sp. z o.o. in Bałcyny (53 • 35 46.4 N, 19 • 51 19.5 E; 136.9 m above sea level). A temperate climate and glacial landform characterize the region. In Bałcyny, soils of the Luvisols type are the most common. Each experiment was established on a soil that satisfied the species requirements of the test plant. Basic soil characteristics prior to the start of each experiment are shown in Table 3. Before the start of the SW-2016 experiment, the soil was also tested for PTE total content. It contained, on average: 1.56 (max 5.655) mg kg -1 As, 0.140 (max 0.687) mg kg -1 Cd, 22.4 (max 26.3) mg kg -1 Cr, 8.94 (max 20.54) mg kg -1 Ni, and 1.67 (max 9.90) mg kg -1 Pb. The meteorological conditions in the period of the study are presented in Table S2. The weather conditions during the 2016 growing season were favorable for spring wheat development. Warm weather and spring rainfall (late April and early May 2016) resulted in even plant emergence. Further intensive wheat development from tillering to heading continued under favorable moisture and thermal conditions (May, June 2016). During the setting and filling of grain, abundant precipitation was observed, but its uniform distribution in time had a beneficial effect on reserve accumulation.
Excessive rainfall at the end of spring wheat vegetation (July 2016) delayed grain maturation. The average monthly air temperatures during the 2016/17 growing season did not differ strongly from the averages established for 1981-2010, while the precipitation distribution differed slightly from that typical for the region. October and November 2016 were slightly cooler than expected, with increased precipitation. December 2016 was relatively warm and rainy. The cooling in the autumn months came gradually, which contributed to the proper winter wheat crop preparation for overwintering. In January 2017, the air temperatures were lower than on average for 1981-2010, and the precipitation was scarce.
The snow cover only increased in February. Winter wheat did not experience the negative effects of the winter season. March was already warm and rainy. April also precipitated abundantly, although it was cooler than usual. These conditions delayed the sowing of spring wheat and then its emergence. A slightly drier May 2017 was followed by rainy periods in June and July and the beginning of August. Such conditions resulted in prolonged ripening and made harvesting the crop on time difficult. Weather conditions, by regulating the soil water content, can determine the availability of P and other elements to plants [73], influence the activity of microorganisms introduced with the biofertilizer [74], and also affect the biological soil activity [75]. Abundant rainfalls can promote the leaching of nutrients and PTE from the topsoil into groundwater or aquatic ecosystems [76].

Grain Yield and Yield Structure Components
The wheat grain yield was evaluated in each of three experiments, based on the amount harvested from individual experimental plots, with the results calculated for 1 ha area and grain moisture of 12%. The wheat yield structure parameters were determined as follows: spike density per 1 m 2 was counted with the frame method prior to wheat harvest, grain number per spike was based on measurements on 25 wheat plants sampled from each plot before harvest (spikes threshed and grains counted manually), and 1000 grain weight (TGW) was derived based on grain samples (about 1 kg) collected during the harvest.

Soil pH
In all experiments, soil pH was investigated on three dates: (1) before the start of the experiment; (2) at the wheat tillering stage (winter wheat has reached this stage in spring); (3) after wheat harvest. Soil samples were taken from the 0-30 cm soil layer using a hand-held twisting probe (Egner's soil sampler). Soil material was collected from each plot separately, from 30 points distributed evenly throughout the plot surface, thereby gaining a sample of approximately 1 kg from a single plot. The collected soil material was dried at room temperature for several days and then thoroughly mixed and sieved. Afterwards, the separated 300 g portions were submitted to the Chemical and Agricultural Station in Olsztyn (Poland), where the pH in 1 M KCl was assayed by potentiometry [77].

Total Element Content in Soil
The total content of C, N, P, K, and Mg was determined in three experiments before their start (for baseline soil characteristics), while the content of As, Cd, Cr, Ni, and Pb was investigated in the SW-2016 experiment on two dates, that is: (1) before the start of the experiment; and (2) after wheat harvest. Sampling and sample preparation were as described in Section 2.4.2. Soil portions of about 300 g each were submitted to the chemical laboratory (accreditation number AB 696) for elemental analysis.
The C and N contents in soil were determined using the Vario Macro Cube Elementar (C,H,N) analyzer (Elementar Analysensysteme, Langenselbold, Germany). Dphenylalanine (C = 65.44%; N = 8.48%) was used as a standard solution. The contents of other elements were determined using an inductively coupled plasma-optical emission spectrometer (ICP-OES with a pneumatic nebulizer with an axial view-iCAP Duo Thermo Scientific, USA). An appropriate mass (0.5 g) of soil samples was digested in Teflon vessels (microwave oven Milestone MLS-1200, Sorisole, Bergamo, Italy) with 10 mL of aqua regia. After mineralization, all samples were diluted to 50 mL and then subjected to multi-elemental analyses using ICP-OES. Elemental determination was accomplished following all principles of measurement traceability. Certificated reference materials were also used to check the quality and metrological traceability. The detection levels (DL) of P, K, Mg, As, Cd, Cr, Ni and Pb in the soil material were 3.59, 2.55, 1.17, 0.5, 0.01, 0.035, 0.25, and 0.15 mg kg -1 , respectively [78].

Available Phosphorus (P) Content in Soil
The content of available P in soil was assayed in three experiments after the test crops were harvested. Sampling and sample preparation were as described in Section 2.4.2. The amount of available P present in the soil was determined in liquid soil extracts. The extract was carried out as follows: 1 g of soil and 50 mL of the extraction solution diluted two times were weighed into an Erlenmeyer flask and then shaken for 1.5 h on a 40 rpm shaker. According to the method described elsewhere [78], the obtained filtrate was subjected to mineralization and determination on ICP-OES. The extraction solution was prepared as follows: 120 g of calcium lactate was weighed into a 1 L volumetric flask, 750 mL of water and 40 mL of hydrochloric acid were added and heated, and then after the dissolution of the salt, one/two drops of chloroform were added, and the flask was then filled with the water till the mark.

Abundance of Heterotrophic Bacteria and Fungi
Soil samples for analyses were taken from three experiments on two dates: (1) at wheat tillering and (2) at wheat heading. Sampling proceeded as described in Section 2.4.2. Each time, before switching to the next plot, soil samplers were sterilized with 96% ethanol (Chempur, PiekaryŚląskie, Poland). Directly after collecting, each soil sample was thoroughly mixed with sterility maintained. Afterwards, small batches of soil material from each sample were placed in sterile 120 mL plastic pots, which were immediately transported to the microbiological laboratory. Heterotrophic bacteria were cultured on Tryptic Soy Agar (TSA, Merck KGaA, Darmstadt, Germany) and fungi on a Rose-Bengal Chloramphenicol (RBC, Merck KGaA, Darmstadt, Germany) agar (Table S3). The media were autoclave-sterilized (ASVE, Spółdzielnia Mechaników SMS, Warsaw, Poland) at 121 • C for 20 min, and were then cooled to 45-50 • C, thoroughly mixed and poured in the amount of 10 mL onto Petri plates with soil solution previously deposited on them (1 mL of 10-3, 10-4 and 10-5 dilutions). Each dilution was prepared in duplicate. Heterotrophic bacteria were incubated at 30 • C for 72 h, and fungi were incubated at 28 • C for five days. The emergent colonies of heterotrophic bacteria and fungi were counted (colony-forming units, CFU), calculated per 1 g of soil and log-transformed (Log10(CFU + 1)).

Calculations and Statistical Analysis
Based on PTE content in biobased fertilizers (Table 1; Cd content below DL was replaced by 0.01 mg kg -1 ), the PTE input per 1 ha with these agrochemicals and the potential increase in soil PTE content (per 1 kg of soil) were calculated. The weight of dry soil from the 0-30 cm-deep layer and 1 ha area was assumed to be 4500 t.
The data on wheat productivity and soil properties were processed using variance analysis (ANOVA) when its assumptions were met or the alternative Kruskal-Wallis test when ANOVA assumptions were not met. Verification of the distribution normality of variables and variance homogeneity was performed using the Shapiro-Wilk W test and Levene's test, respectively. Duncan's test or a multiple comparisons test were then applied to assess differences between objects. A median and a maximum value (Max) were determined for soil PTE content. In all statistical calculations for PTE, their contents below DL were replaced by values equal to the DL. The relationships between grain yield and yield structure elements and between grain yield and available P content in soil were expressed by simple correlation coefficients. The analyses were performed using Statistica 13.3 software [79].

Grain yield and Yield Structure Elements
The ultimate goal of fertilization is to increase yields [35]. In the current study, soil P reserves in experimental fields without P fertilization (P 0 ) allowed wheat to produce yields of 6.02, 4.36 and 6.51 t ha -1 in experiments SW-2016, SW-2017 and WW-2017, respectively (Table 4). In each experiment, regardless of the fertilizer used, P application only at a dose of 17.6 kg ha -1 (SP 1 , AF 1 , and BF 1 ) resulted in a significant yield increase compared to the relevant control treatment. The validity of increasing the P dose to 26.4 kg ha -1 was statistically confirmed only for SP application (SP 2 versus SP 1 ) in the WW-2017 experiment. However, in many cases, although a tendency to increase yield with increasing P dose was perceptible, the application of AF 3 in the SW-2016 experiment and SP 3 in experiments SW-2017 and WW-2017 no longer had only a statistical but also a biological justification. The weak response of wheat to the increase in P doses noted in the current study may have been a consequence of the P richness of the soil environment involved in the trials. Ram et al. [80] did not notice a significant response of wheat yield to the increase in a P dose from 13 to 26 kg ha -1 in an experiment set up on soil moderately rich in P, whereas a higher yielding capacity occurred on P deficient soils. The yield-forming effect of BF was comparable to that of SP applied at the same P dose, while the AF 2 treatment provided significantly lower wheat yield than SP 2 in both the SW-2017 and WW-2017 experiments. Under field conditions, the more beneficial yieldforming effect of BF in relation to AF was mostly manifested by trends (weak or more pronounced) and was only confirmed for BF 2 against AF 2 in the WW-2017 experiment. It was also observed that the beneficial effect of BF compared to AF was more evident under poorer habitat conditions (worse previous crop in SW-2017 and WW-2017, lower soil P content in WW-2017).
Reports on the satisfying yield stimulating effect of fertilizers produced from SSA have been published previously [64,68,81]. However, under-performance was also noted [82,83]. The meta-analysis performed by Huygens and Saveyn [36] suggests that P-fertilizers derived from SSA may compare in agronomic efficiency with mined and synthetic P-fertilizers, but it is dependent on the raw material applied, possible post-combustion manufacturing processes and the length of the plant growing season. Despite their low water solubility, recycled P-fertilizers are considered to be a valuable alternative for traditional ones in conventional and organic European agriculture [35,36].
Studies to date on the role of PSM in improving the yield-forming efficiency of fertilizer P sources do not yet provide clear answers. In relation to conventional P sources and fertilizers, both positive effects [60,80,84,85] and lack of the expected interaction were noted [84,86]. Although it was proven in laboratory research that PSM are efficient in solubilizing P from SSA [62], many studies, both pot [84,86,87] and field [88,89], demonstrate the poor contribution of bioeffectors (microbes) in increasing the yield-forming efficiency of SSA-based P-fertilizers. The findings from the current study are promising, especially from the WW-2017 experiment, although they are also in line with the opinions that more studies are required to understand agronomic effects of SSA [90] and to fully recognize the necessity, opportunities and conditions for increasing these effects through PSM activity [31,88,89]. Since many technologies are at the pilot or laboratory scale research stage [37], comprehensive field studies are in particular demand [31,45].
In the current study, the tested fertilizer treatments did not differentiate wheat spike density and grain number per spike in all the experiments, while P fertilization usually increased 1000 grain weight (TGW) relative to P 0 (except for SP 1 , AF 1 , and AF 2 in experiment WW-2017-only an increasing tendency was observed). The applied fertilizers and P doses influenced TGW in the same way. In all three experiments, among the elements of yield structure, TGW was the strongest contributor to yield formation (Table 5). In experiment SW-2016, the yield was also positively correlated with spike density, with no relationship with grain number per spike. Spike density and grain number per spike did not correlate with yield in the SW-2017 and WW-2017 experiments. The literature is not clear on the contribution of yield structure components in forming the yield of individual cereal species [91]. It is argued that unambiguous determination of the effect of yield structure components on yield is difficult because it is determined by both genetic factors (species, cultivar) and environmental conditions [92].

Soil pH
Before the start of the SW-2016 and SW-2017 experiments (Table 3), soil pH in the respective fields fell within the range corresponding to the highest P bioavailability (5.5-7.0) [93]. In experiment WW-2017, the initial soil pH was below 5.5, indicating that P availability was limited by P incorporation into aluminum (Al) or iron (Fe) compounds [94,95].
No differences in pH induced by applied P fertilization were found in any experiment either at the tillering stage of wheat or after wheat harvest (Table 6). In contrast, pot experiments conducted by Rosyadi [96] and Zalewska et al. [97] showed an increase in soil pH after test plant harvest as a result of SSA application compared to no P treatments and conventional mineral P fertilizers (triple superphosphate (TSP) and monocalcium phosphate, respectively). Rosyadi [96] explains these differences by way of the higher Ca load in SSA and the nature of the ashing process, where CO 2 is removed and alkaline CaO is generated, as well as by the potential release of protons during TSP hydrolysis. This deacidifying/alkalizing effect of SSA [98] was not pronounced in the present studies conducted under field conditions, i.e., in an open, only partially controlled system. Similar results were obtained by Jastrzębska et al. in field studies with other SSA-based fertilizers [99,100]. It is also worth noting that, in the present study, no change in soil pH was observed under the influence of B. megaterium bacteria introduced with BF. Based on B. megaterium biology [101] and previous laboratory studies [62] indicating that the growth of B. megaterium cultured on SSA was accompanied by a lowering of medium pH, the possibility of a decrease in soil pH resulting from the activity of B.megaterium introduced with BF was assumed. However, it seems that the abundance of B. megaterium introduced with BF was too low to affect the soil pH value under field conditions in confrontation with the soil buffering properties [102], or with the possible bacterial response to the encountered adverse environmental conditions (fast sporulation [103]). In other studies by Jastrzębska et al. conducted in field conditions, SSA-based fertilizers activated by B.megaterium did not increase the abundance of B.megaterium in the soil [104] and did not change the soil pH [99]. Similarly, Zhao et al. 2021 [85] reported that, compared to conventional fertilization (chemical fertilizer + manure), the addition of B.megaterium did not change soil pH after cucumber harvest.
In experiment SW-2016, soil pH was statistically stable during the growing season, whereas differential seasonal changes in soil pH, independent of fertilizer type and P rate, were found in experiments SW-2017 and WW-2017. Although soils have their specific buffering capacities [102], seasonal fluctuations in soil pH of 0.2-0.3 of a pH unit are, in fact, not unusual [105]. Many factors are cited among their causes, including fertilizer type, rate and time of application, organic matter, plant root and bacterial activity, soil moisture [105], plant uptake of nutrients in the form of cations and anions [106], and nutrient leaching or volatilization (depending on the element and the form of its compounds) [107,108]. A more detailed discussion of seasonal changes in soil pH is presented in the Supporting Information.

Content of Potentially Toxic Elements in the Soil
The contents of major potentially toxic elements (PTE), i.e., As, Cd, Cr, Ni and Pb, that were determined in the soil in the SW-2016 experiment (Table 7), fell within the ranges considered natural for Polish and regional conditions and did not exceed the permitted levels for arable lands in Poland (see Table S4). Even the maximum values found in the present study were within the geochemical background, except for Cd, of which the maximum content was slightly higher (1.149 vs. 1.000 mg kg -1 ). The median values for all studied PTE were lower than the averages. Since PTE were present in the elemental composition of the biobased fertilizers (Table 1), the possibility of an increase in soil PTE content as a result of AF and BF application (especially compared to the P 0 treatment) was considered. The amounts of PTE that entered the soil with AF and BF fertilizers were so small ( Table 8) that the enrichment of soil PTE ended up being negligible for statistical analysis. No changes in soil PTE content compared to the initial state were proven, nor were differences due to the influence of the fertilizer factor. Regardless of the P dose, AF and BF, just like SP, did not significantly change the content of these elements compared to the control (P 0 ). Previous reports indicate a possible increase in soil PTE content as a result of the longterm application of P fertilizers, both conventional and from renewable resources, if they carried high PTE amounts [110,111]. Not only the amount of PTE carried with the fertilizer, but also which PTE the fertilizer contains seems to be important. For example, it is believed that under normal cropping practices, As accumulation in soils is insignificant [110,112], while the application of Cd-containing P-fertilizers could, over time, cause Cd to accumulate in soil and therefore increases the accompanying risks [110]. On the other hand, according to Uprety et al. [113], common cropping practices with the application of organic and inorganic fertilizers do not substantially enrich soils in As, Cd, Cr, Ni, and Pb, even after they have been applied for more than 50 years.
Chen et al. [110] found an increase in soil Pb content after a 10-year application of 2.35-47.0 g Pb along with 25-400 kg ha -1 P (statistically the same increase regardless of the P dose), but no increase in As and Cd content when applied at rates up to 42.2 g and 1.01 g (with 200 kg ha -1 P) and in Cr and Ni content when applied at a rate up to 42.4 g and 21.7 g (with 400 kg ha -1 P), respectively. A point worth noting is that, except for Pb, the amounts of PTE introduced into the soil together with AF and BF fertilizers in the present study (Table 8) were substantially lower. Another study by Jastrzębska et al. [114] based on several one-year experiments with different SSA-containing fertilizers also brought promising findings; however, considering the nature of PTE, further long-term studies are called for.

Available Phosphorus (P) Content in Soil
According to the standard Polish classification of plant-available P by Egner-Riehm [115], the content of available P in soil (Table 9) should be classified as low to medium in experiment SW-2016, as a medium in experiment SW-2017, and as low in experiment WW-2017. The latter was due not only to the relatively low total P content in the soil but also to the soil pH limiting P bioavailability [95].
In all three experiments, P fertilization increased or tended to increase the available P content in the soil. Only a trend toward enhanced available P was observed in experiments SW-2016 and WW-2017 after the application of SP 1 , AF 1 , AF 2 , AF 3 , and BF 1 treatments. Higher rates of SP (SP 2 , SP 3 ) and BF (BF 2 , BF 3 ) in these experiments, as well as all P fertilization treatments in experiment SW-2017 resulted in a significant increase in available P in the soil. In all experiments, the highest available P content in soil was recorded after SP 3 application. This is mostly the result of applying a relatively high dose of P in a readily available form [116] that was not fully utilized by the plants (Table 4).
When comparing the same P amounts (doses) introduced with different fertilizers, lower amounts of available P were found after the application of AF 3 and BF 3 in SW-2017 and after AF 3 in WW-2017, compared to SP 3 , respectively in the relevant experiments. The differences for SP 3 versus AF 3 are explained by the lower solubility of P compounds in the waste raw material [44], while the differences for SP 3 versus BF 3 are rather due to the unused part of available P by plants after SP 3 application (see Table 4). In other cases, the available P content under the influence of AF and BF did not differ from that formed by SP applied at the same P dose. The performance of BF against AF did not prove spectacular, but it should be rated as moderately optimistic. In the SW-2016 experiment, the solubilizing activity of B. megaterium contained in BF was shown only by a tendency to increase available P content compared to the effect of AF applied at the same P dose. In the SW-2017 experiment, a positive tendency under BF 1 versus AF 1 was also noted, but a significant increase in available P content was observed under BF 2 and BF 3 in comparison to AF 2 and AF 3 , respectively. In contrast, in the WW-2017 experiment, BF 1 and BF 2 treatments resulted in a trend of higher available P content relative to AF 1 and AF 2 , while higher available P content was confirmed under BF 3 relative to AF 3 . These effects are also related to the fact that some portion of the P dissolved by B. megaterium was not used by the plants to build a higher yield. However, in all experiments, a positive correlation was found between the content of available P in the soil after wheat harvest and wheat grain yield (Table 10). Table 10. Relationship between available P content in soil and wheat grain yield-simple correlation coefficients. The high solubilization activity of B. megaterium has been proven in previous studies [117], as well as its contribution to increasing available P in soil [118]. This bacterial strain is mentioned among commercially used species of PSM [119]. In laboratory tests, Wyciszkiewicz et al. [49] demonstrated the potential of using B. megaterium to solubilize P compounds of SSA, although the solubilization efficiency was lower than with animal bones as raw material. Since the rate and the intensity of the P solubilization process are dependent on many factors (e.g., pH, temperature, moisture and others) [119], this activity of B. megaterium can be strongly modified under field conditions where many environmental parameters are uncontrolled, and over the period of fertilization which is often unfavorable for microbes. The results of the current study are consistent with the claim that beneficial soil bacteria under nutrient-poor conditions are more likely to act as nutrient solubilizers (see WW-2017 in Table 9) but do not prove their performance as plant growth enhancers under nutrient-rich soil conditions (see Tables 4 and 9) [120]. For comparison, Zhao et al. [85] reported that, relative to conventional fertilization (chemical fertilizer + manure), the addition of B. megaterium significantly increased cucumber yield and the dry weight of cucumber fruit and roots without increasing the soil available P content. It can also be assumed that the abundance of bacteria introduced into the soil with BF in the current study was too low, or they were not competitive enough to proliferate efficiently and intensify P release. Raymond et al. [89] suggested that inoculation with the P-solubilizing fungus Penicillium bilaiae did not prove to be a suitable strategy to enhance P availability from SSA, probably due to the limited competitiveness of the fungus in the soil.

SW
Further research on PSM strain selection and their ratio in waste-based fertilizer formulations, as well as a better understanding of PSM interactions with feedstock P under natural (field) conditions would be supportive in developing this alternative Pfertilizing approach.

Abundance of Heterotrophic Bacteria and Fungi
The numbers of heterotrophic bacteria and fungi found in the soil of the conducted experiments (Table 11) were within the ranges corresponding to the microbiological characteristics of Polish arable soils [121]. The tested P fertilization treatments did not differentiate the abundance of these microorganisms in any of the experiments and on none of the analysis dates adopted. Studies by other authors have shown that the introduction of P into the soil in the form of mineral fertilizers, as well as the type of fertilizer, did not change the abundance of soil microorganisms relative to the control (P 0 ) [122][123][124][125]; however, both mineral P application and fertilizer type (e.g., phosphate rock vs. triple superphosphate) altered the structure and diversity of the microbial community [124][125][126]. Moreover, for the latter, contradictory effects were observed [125].
Although the relationships between native soil microorganisms and introduced microorganisms (their compatibility or incompatibility) are not fully recognized [127], some studies have shown that both the abundance and structure of indigenous communities can be altered by allochthonous organisms [128] through competition, activity stimulation and other microbe-microbe interactions [61]. In the present study, no change in the abundance of heterotrophic bacteria and soil fungi under the influence of B. megaterium introduced into the soil with BF was observed in any of the field experiments. This may be due to the relatively low abundance of B. megaterium in the BF formulation. Moreover, the activation of the lyophilized cells and their proliferation strongly depended on the environmental conditions, mainly the proper soil moisture and temperature. In other studies by Jastrzębska et al. [99,100], similar results were obtained when B. megaterium was a component of suspension or granular fertilizers from SSA and/or animal bones. In contrast, Ali et al. [129] reported that Bacillus subtilis applied as a biofertilizer alone or in compilation with triple superphosphate increased the number of bacteria in soil under wheat. Zhao et al. [85] found that the application of B. megaterium improved the richness of bacterial and fungal communities, increased the relative abundances of the beneficial bacterial genera and fungal orders, and suppressed the development of pathogenic bacterial genus in soil under cucumber grown in the plastic shed system. It should be added that in the studies by Ali et al. [129] and Zhao et al. [85], active bacteria were applied in large quantities. Analysis of the structure and biodiversity of soil microbial communities under the influence of fertilizers and biofertilizers from waste biomass is a further research challenge for the authors of the present study.
The differences in bacterial abundance between analysis dates found in the present study resulted from the rainfall quantity occurring in the period prior to analysis in each experiment and from the corresponding soil moisture. Seasonal variation in fungal abundance was not observed. Fungi are considered more tolerant of soil drought than bacteria. It is accepted that bacteria show no activity at less than 30% environmental moisture, while fungal growth ceases at less than 15% moisture [75].

Conclusions
Under field conditions, the effectiveness of BF fertilizer was comparable with SP fertilizer regarding the yield-forming potential. In comparison with SP and BF fertilizers, the AF fertilizer (without bacteria) usually had a slightly weaker yield-forming ability. AF and BF fertilizers resulted in the same or a lower level of soil available P as SP applied at the same P rate. The slightly higher effectiveness of BF relative to AF at increasing yield and soil available P was evident under poorer habitat conditions. Both AF and BF, applied at the P rate up to 35.2 kg ha -1 (similar to SP), did not affect the pH level of soil under the test plant, did not increase As, Cd, Cr, Ni, and Pb in the soil, and did not alter the abundance of heterotrophic bacteria or fungi in the soil. These findings demonstrate that such recycled agrochemicals can be an alternative to conventional Pfertilizers under phosphate rock shortage or at least partially replace them. The tested fertilizers are admittedly less P-rich than commercial fertilizers but additionally contain numerous macro-and micronutrients. The comparable results obtained for AF and BF put into question the need for biological activation of the waste biomass-based preparation. However, the advantageous trends outlined should inspire further work towards increasing the proportion of B. megaterium in BF or searching for more effective biological activators. This promising alternative P-fertilizing approach also needs to be verified in long-term field studies, especially with respect to the potential accumulation of PTE introduced into the soil with waste-based fertilizers.