Cannabidiolic acid in Hemp Seed Oil Table Spoon and Beyond

Cannabidiolic acid (CBDA) is the main precannabinoid in industrial hemp. It represents a common constituent of hemp seed oil, but mainly abundant in the aerial parts of the plant (including their processing waste). Thus, the optimization of fast and low-cost purification strategies is mandatory, as well as a deep investigation on its nutraceutical and cosmeceutical properties. To this purpose, CBDA content in hemp seed oil is evaluated, and its recovery from wasted leaves is favorably achieved. The cytotoxicity screening towards HaCaT cells, by means of MTT, SRB and LDH release assays, suggested it was not able to decrease cell viability or perturb cell integrity up to 10 μM concentration. Thus, the ability of CBDA to differentially modulate the release of proinflammatory cytokines and chemokines mediators has been evaluated, finding that CBDA decreased IFN-γ, CXCL8, CXCL10, CCL2, CCL4 and CCL5, mostly in a dose-dependent manner, with 10 μM tested concentration exerting the highest activity. These data, together with those from assessing antimicrobial activity against Gram(+) and Gram(−) bacteria and the antibiofilm formation, suggest that CBDA is able to counteract the inflammatory response, also preventing bacteria colonization.


Introduction
Nutrition greatly benefits our health, and the diet, when well-balanced and properly enriched in bioactive substances, is capable of providing, beyond energy and nutrients, phytobioactive agents, useful to meet the needs of the body as a whole and of individual tissues specifically [1]. The food nutritional and nutraceutical value could nourish the skin [2], which is characterized by a high turnover, also from the inside, favoring a new form of nutrition or rather nutritional supplementation, known as nutricosmetic, which aims to optimize the needs of the skin satisfying it [3]. Thus, through a joint approach between dermo-cosmetic and food research, food supplements are prompt to promote skin hydration, also improving skin tissues fluidity, and increasing skin tone brightness and uniformity, and delaying skin aging [4][5][6]. In this context, food rich in essential fatty acids (EFA) could be a useful player in epidermal integrity maintenance, so much so that and it was found that the topical application of plant oils is a strategy to protect skin, retaining

CBDA Cytotoxicity in HaCaT Cells
In order to hypothesize the nutraceutical use of CBDA for skin, the cytotoxic effect of CBDA was preliminarily evaluated by means of three different assays towards the HaCaT cell line, which derives from normal human skin and it is spontaneously immortalized [28]. Evaluating CBDA cytotoxicity in HaCaT cells at ten different concentrations (from 0.1 to 100 µM), it was observed that it was able to exert a dose-dependent inhibition of mitochondrial redox activity. The compound appeared to be non-toxic when tested at low concentrations (up to 10 µM), whereas it was able to reduce dehydrogenase activity by 40.7%, 57%, and 84% at 25, 50, and 100 µM (Figure 2A). This was not in line with Martinenghi et al. [29], who observed that CBD showed dose-dependent killing up until 16 µg/mL, where at higher doses the cytotoxicity was stabilized at 50%. The increase in concentration appeared to affect cell morphology, pointing that in dosages above 10 µM, cells were similar to balloon-like bubbles [30].
Recently, an hemp seed apolar extract, with CBD and CBDA as the main compounds, was reported to exert anti-inflammatory and anti-lipogenic activity in a Propionibacterium acnes-triggered inflammation and lipogenesis model [35]. Considering that both CBDA and CBD have structurally low binding affinity to the endocannabinoid receptors, the less polarity of CBDA was not limiting for microemulsion formulations for transdermal delivery [36].  When sulforhodamine B assay was performed, CBDA at 10 and 25 µM was observed to decrease by 26.5% and 35.7%, respectively, the total protein mass, which is related to cell number, whereas at the lowest tested dose there was no inhibition of cell viability ( Figure 2B). However, the inhibitory effect does not increase in proportion to the dose increase, with an inhibition of 55.3% occurring in cells treated with CBDA at 100 µM. At this latter concentration, as well as at 50 µM, a marked lactate dehydrogenase (LDH) release was further detected ( Figure 2C). The intracellular LDH enzyme was from damaged cells and its content is an indicator of cell membrane integrity. The effect observed in the highest concentration of CBDA-tested HaCaT cells was unrelated to what was recently found testing an extract of Cannabis sativa L. herb [31]. The neutral derivative of CBDA was previously tested on keratinocytes and, according to our data, no cytotoxic effects were at 1, 5, 10, and 20 µM after 6, 12, and 24 h [32]. Furthermore, it was reported that CBD was able to induce keratinocyte differentiation, and skin development [33]. The extensive investigation of the CBD efficacy in maintaining skin health [34] does not find a counterpart in its precannabinoid, which has attracted less attention, probably due to its tendency to decarboxylate or, more simply, to the lack of awareness of its presence and relative abundance. Thus, only indirect data are available on CBDA activity in skin cells. Recently, an hemp seed apolar extract, with CBD and CBDA as the main compounds, was reported to exert anti-inflammatory and anti-lipogenic activity in a Propionibacterium acnes-triggered inflammation and lipogenesis model [35]. Considering that both CBDA and CBD have structurally low binding affinity to the endocannabinoid receptors, the less polarity of CBDA was not limiting for microemulsion formulations for transdermal delivery [36].

CBDA Modulated Cytokines and Chemokines in HaCaT Cells
Since the HaCaT cell line is a reliable model to screen for new anti-inflammatory compounds for skin diseases, and keratinocytes are involved in the initiation and perpetuation of the cutaneous inflammatory response [37], the study of the ability of CBDA to differentially modulate the release of proinflammatory mediators was carried out ( Figure 3A). For this purpose, HaCat cells were first treated with the non-toxic doses 2.5, 5, and 10 µM. Cytokines, including chemokines, play a critical role in mediating inflammation, and they are involved in cell communication, also regulating immunity, and cell activation, migra-tion, and proliferation [38]. The CBDA concentration appeared to be determinant in the response observed. In fact, it was observed that CBDA inhibited dose-dependently IL-1ra (IL-1 receptor antagonist), a regulator of IL-1 signaling, and a competitive antagonist of the IL-1 agonist receptor (IL-1R1) [39]. The inhibition of the pleiotropic cytokine interleukin-9 (IL-9) also occurred. T cells and mast cell growth factor are implicated in allergic and autoimmune disorders, parasitic infections, and antitumor immunity [40]. Recently, it was observed that an increased IL-9 secretion is related to atopic dermatitis anthogenesis [41]. Moreover, CBDA was able to decrease IFN-γ, CXCL8, CXCL10, CCL2, CCL4 and CCL5. With the only exception of the effect on CXCL8, towards which a similar response was from CBDA 2.5 and 5 µM treatments, all the other mediators were extensively inhibited in a dosedependent manner, with CBDA at 10 µM exerting the highest activity as also evidenced by the heatmap ( Figure 3B). CBDA appeared to effectively exert the downregulation of IFN-γ, which is the dominant cytokine in inflamed skin, and in the skin of patients with atopic dermatitis, where it mediates also the upregulation of CCL5 expression [42]. The latter is a proinflammatory chemokine, formerly also known as RANTES, whose expression increases in lesioned skin [43], especially in lesions of psoriasis [44]. CCL5 expression detected in the supernatant of HaCaT cells was reduced by 20%, following CBDA 10 µM treatment. The role of IL-8 in psoriatic skin, as well as in atopic dermatitis, also was elucidated [45], and effect of CBDA at 10 µM, which was able to reduce IL-8 by 32%, is non-negligible. IL-8 is involved and produced in many inflammatory reactions, and keratinocytes are a rich source of it [46]. While CCL4, also known as macrophage inflammatory protein-1β (MIP-1β), was decreased by CBDA treatment in the HaCaT cells, CCL2 (MCP-1, Monocyte chemoattractant peptide-1), a chemokine acting as mediator in allergic rhinitis and psoriasis [47], and able to mediate inflammation after tissue damage in various organs, was completely pulled down, with a three-fold decrease compared to untreated cells already following the treatment with CBDA 2.5 µM. Finally, a marked decrease was for CXCL10, claimed as marker of vitiligo and psoriasis [48,49]. Contrary to what previously demonstrated for CBD, an increase in GM-CSF was found, which was poorly contained in untreated cells, as well as in CBDA 2.5 and 5.0 µM treated cells. Vascular endothelial growth factor (VEGF), which was reported to modulate HaCaT cells adhesion [50], appeared to be stimulated by the highest CBDA concentration, which further slowed down the platelet-derived growth factor (PDGF), commonly involved in epithelial hyperproliferation [51]. Recent findings showed a similar cytokine and chemokine behavior in CBD-treated blood of depressed patients, concluding that cannabidiol exerts dose-dependent immunomodulatory effects, and that at higher concentrations it was able to worsen inflammation [52].

Antibacterial Activity of CBDA: MIC and MBC Determination
Disturbance in the inflammatory and immune systems mediated by the above-considered cytokines and chemokines, leads very often to episodes of opportunistic skin bacterial infections compromising skin health [53]. There is the urgent need to discover compounds acting on both the inflammatory response and the prevention of bacteria colonization. Therefore, the antibacterial activity of CBDA, also considering recent literature data [29,54] was tested ranging from 0.064 to 64 µg/mL against two Gram-negative bacteria such as P. aeruginosa ATCC 27853 and E. coli ATCC 13762 and two Gram-positive bacteria such as S. aureus ATCC 6538 and E. faecalis ATCC 29212. The MIC values of CBDA ranged from 0.5 to 4 µg/mL for all the microorganisms tested, with the exception of P. aeruginosa that is resistant up to a concentration of 8 µg/mL (Table 1). CBDA also showed bactericidal effects against both Gram-negative and Gram-positive bacteria used for the study as no growth of bacteria was observed at the different concentrations reported in Table 1 (MBC values).

Antibacterial Activity of CBDA: MIC and MBC Determination
Disturbance in the inflammatory and immune systems mediated by the above-considered cytokines and chemokines, leads very often to episodes of opportunistic skin bacterial infections compromising skin health [53]. There is the urgent need to discover compounds acting on both the inflammatory response and the prevention of bacteria colonization. Therefore, the antibacterial activity of CBDA, also considering recent literature data [29,54] was tested ranging from 0.064 to 64 μg/mL against two Gram-negative bacteria such as P. aeruginosa ATCC 27853 and E. coli ATCC 13762 and two Gram-positive bacteria such as S. aureus ATCC 6538 and E. faecalis ATCC 29212. The MIC values of CBDA ranged from 0.5 to 4 μg/mL for all the microorganisms tested, with the exception of P. aeruginosa that is resistant up to a concentration of 8 μg/mL (Table 1). CBDA also showed bactericidal effects against both Gram-negative and Gram-positive bacteria used for the study as no growth of bacteria was observed at the different concentrations reported in Table 1 (MBC values).

Evaluation of Antimicrobial Activity of CBDA by Time-Killing Assay
The time killing studies of CBDA against E. coli ATCC 13762, S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 and E. faecalis ATCC 29212 were performed. Different CBDA concentrations were used (i.e., MIC, 2XMIC, 4XMIC values), and bacteria were analyzed at different incubation times (1, 2, 3, 5 h); data obtained are shown in Figure 4.
During the first incubation hours (2 and 3 h), CBDA exhibited a strong reduction of the growth of E. coli ATCC 13762, S. aureus ATCC 6538, E. faecalis ATCC 29212 and P. aeruginosa ATCC 27853 strains at concentrations of CBDA as shown by the MIC value ( Figure 4A-D). At prolonged incubation times, a slight recovery of growth for the following three bacterial strains was observed for the MIC value: E. coli ATCC 13762, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 ( Figure 4A-C). On the contrary, for E. faecalis ATCC 29212, no growth recovery was observed ( Figure 4D). Regarding concentrations of CBDA of 2X and 4X MIC, at prolonged incubation times a recovery of growth and vitality was observed only for E. coli and S. aureus ( Figure 4A,B). the growth of E. coli ATCC 13762, S. aureus ATCC 6538, E. faecalis ATCC 29212 and P. aeruginosa ATCC 27853 strains at concentrations of CBDA as shown by the MIC value ( Figure 4A-D). At prolonged incubation times, a slight recovery of growth for the following three bacterial strains was observed for the MIC value: E. coli ATCC 13762, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 ( Figure 4A-C). On the contrary, for E. faecalis ATCC 29212, no growth recovery was observed ( Figure 4D). Regarding concentrations of CBDA of 2X and 4X MIC, at prolonged incubation times a recovery of growth and vitality was observed only for E. coli and S. aureus ( Figure 4A

Biofilm Formation
CBDA was tested for inhibition of biofilm formation using two different assays, quantified through staining with crystal violet ( Figure 5A) and staining and visualization with a microscope ( Figure 5B). Different concentrations (MIC, 2X MIC, 4X MIC) of CBDA were used, as shown in Figure 5A,B. CBDA was able to inhibit biofilm formation from all bacteria strains at concentrations as low as the MIC value ( Figure 5A). In addition, during the biofilm formation phase, CBDA showed maximum activity at concentrations corresponding to 4X MIC for each microorganism ( Figure 5A). Biofilms formed by S. aureus and P. aeruginosa after 24 h of static culture were stained with a LIVE/DEAD stain and

Biofilm Formation
CBDA was tested for inhibition of biofilm formation using two different assays, quantified through staining with crystal violet ( Figure 5A) and staining and visualization with a microscope ( Figure 5B). Different concentrations (MIC, 2X MIC, 4X MIC) of CBDA were used, as shown in Figure 5A,B. CBDA was able to inhibit biofilm formation from all bacteria strains at concentrations as low as the MIC value ( Figure 5A). In addition, during the biofilm formation phase, CBDA showed maximum activity at concentrations corresponding to 4X MIC for each microorganism ( Figure 5A). Biofilms formed by S. aureus and P. aeruginosa after 24 h of static culture were stained with a LIVE/DEAD stain and imaged by Cell Discoverer 7, Zeiss ( Figure 5B). Upon examination of the images, the biofilms formed by untreated bacteria were noticeably thicker and bigger in volume than that those formed by treated bacteria.
The inhibition of biofilm formation induced by CBDA was evident at concentrations equal to the MIC and the maximum effect was obtained at concentrations corresponding to 4X MIC for both S. aureus and P. aeruginosa. The antibiofilm formation of cannabinoids was shown for CBD, which inhibited Candida albicans biofilm by a multi-target mode of action [55], and in S. aureus, S. pneumoniae, and C. difficile, acting mainly defining membrane disruption [56]. imaged by Cell Discoverer 7, Zeiss ( Figure 5B). Upon examination of the images, the biofilms formed by untreated bacteria were noticeably thicker and bigger in volume than that those formed by treated bacteria. The inhibition of biofilm formation induced by CBDA was evident at concentrations equal to the MIC and the maximum effect was obtained at concentrations corresponding to 4X MIC for both S. aureus and P. aeruginosa. The antibiofilm formation of cannabinoids was shown for CBD, which inhibited Candida albicans biofilm by a multi-target mode of action [55], and in S. aureus, S. pneumoniae, and C. difficile, acting mainly defining membrane disruption [56].

Permeabilization of S. Aureus ATCC 6538 and E. coli ATCC 13762 Membranes induced by CBDA
The integrity of the plasma membranes of S. aureus ATCC 6538 and E. coli ATCC 13762 after incubation with CBDA was assessed ( Figure 6). CBDA-induced permeabilization was evaluated 20, 40 and 60 min after the addition of SYTOX Green and CBDA at concentrations corresponding to MIC, 2X MIC, 4X MIC for each bacterium. CBDA caused an increase in SYTOX Green influx already after 20 min of incubation for both S. aureus and E. coli. The most relevant effect was obtained after 60 min of incubation with CBDA at a concentration equal to the MIC for E. coli and 4X MIC for S. aureus.

CBDA Quantitation in Hemp Seed Oil and Leaf Wastes
Hemp seeds (1.0 g) from Futura 75 variety, collected within ProHemPil Project activities in Campania Region (Italy), were first cryo-dried and ground, and then subjected to ultrasound assisted maceration (Branson UltrasonicsTM BransonicTM M3800-E, Danbury, CT, USA) in n-hexane using the ratio hemp seeds:solvent equal to 1:5. Three extrac-

CBDA Quantitation in Hemp Seed Oil and Leaf Wastes
Hemp seeds (1.0 g) from Futura 75 variety, collected within ProHemPil Project activities in Campania Region (Italy), were first cryo-dried and ground, and then subjected to ultrasound assisted maceration (Branson UltrasonicsTM BransonicTM M3800-E, Danbury, CT, USA) in n-hexane using the ratio hemp seeds:solvent equal to 1:5. Three extraction cycles were carried out (40 min each). After solvent removal by using a rotary evaporator, the UaM hemp seed oil was obtained with an extraction yield equal to 25%. The monovarietal Futura 75 cold-pressing oil was acquired by a local producer. Hemp leaves, collected at seeds harvesting time, also were ultrasound assisted macerated. All the samples were analyzed mainly by means of UHPLC-ESI-QqTOF-MS techniques. A Shimadzu NEXERA UHPLC system (Shimadzu, Tokyo, Japan) equipped with a Luna ® Omega Polar C18 column (1.6 µm, 50 × 2.1 mm i.d, Phenomenex, Torrance, CA, USA) was utilized and a binary solution (A) H 2 O (0.1% HCOOH), (B) CH 3 CN (0.1% HCOOH) was used for separative purposes. The gradient program initiated at 5% B, and linearly ramped up to 55% B in 5 min, to 75% B in other 5 min, and finally to 95% B in one minute. The program held for 1.0 min at 95% B, and then the initial condition was restored and held for another 2 min. The total run time was 13 min, with a flow rate of 0.4 mL min −1 . The injection volume was 2.0 µL. MS analysis was performed using the AB SCIEX Triple TOF ® 4600 (AB Sciex, Concord, ON, Canada). The MS parameters were as follows: curtain gas 35 psi, nebulizer gas 60 psi, heated gas 60 psi, ion spray voltage 4.5 kV, interface heater temperature 500 • C, and declustering potential −75 V. In TOF-MS/MS experiments, collision energy applied was −55 V with a collision energy spread (CES) of 35 V. The instrument was controlled by Analyst ® TF 1.7 software (AB Sciex, Concord, ON, Canada, 2016), while data processing was carried out using PeakView ® software version 2.2 (AB Sciex, Concord, ON, Canada, 2016). The calibration curve of CBDA (y = 2 × 10 8 x + 10 6 ) was prepared, and used to quantize the compound in the matrices. Cannabidiolic acid was purified and chemically characterized according to Formato et al. [27]. Briefly, the adopted protocol involved a solid/liquid extraction accelerated by ultrasounds, with chloroform/n-hexane (1:1, v/v) solution as extractant. Then, normal-phase preparative thin-layer chromatography (PTLC) was applied, eluting with the organic phase of a biphasic CHCl 3 /MeOH/H 2 O (13:7:7, v/v/v) solution, followed by reverse-phase (RP-18) TLC, using a MeCN/H 2 O (4:1, v/v) solution as the mobile phase. CBDA purity was verified by UHPLC-ESI-QqTOF-MS, using the experimental parameters described above. Furthermore, HPLC-UV analysis was performed, by using a HPLC 1260 INFINITY II system, equipped with DAD-UV-visible detector (Agilent, Santa Clara, CA, USA).
The pure compound was stored at −20 • C until cytotoxic and anti-microbial screenings were carried out.

Cell Culture
Human HaCaT epidermal keratinocyte cell line was purchased from ATCC (American Type Culture Collection). Cells were cultured in DMEM high glucose medium supplemented with 10% Fetal Bovine Serum, 50.0 U/mL penicillin, and 100.0 µg/mL streptomycin, at 37 • C in a humidified atmosphere containing 5% CO 2 .

SRB Cell Viability Assay
Cell density determination by sulforhodamine B (SRB) was carried out seeding 1.5 × 10 4 HaCaT cells per well in 96-well plates [57]. Twenty-four hours after seeding, cells were treated with CBDA at 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 25, 50 and 100 µM (final concentration level in the volume of 100 µL), in the medium for 24 h exposure time (12 wells for each treatment). Untreated HaCaT cells were also incubated (12 wells). At 24 h of incubation, after medium removal, cells were fixed with ice-cold trichloroacetic acid (TCA, 10% (w/v), 40 µL) for 1 h at 4 • C. Then, plates were washed with distilled water, and dried. Then, 50 µL of sulforhodamine B (SRB, 0.4% (w/v) in 1% aqueous acetic acid) solution were added to each well, and the plates were incubated at room temperature for 30 min. After the unbound dye removal, by washing the plates with 1% aqueous acetic acid, plates were dried. Thus, the bound SRB was solubilized with unbuffered Tris Base (100 µL; 10 mM, pH 10.5), and the absorbance at 570 nm was measured using a Tecan SpectraFluor fluorescence and absorbance reader. The cell viability inhibition was calculated as a percentage (±standard deviation, SD) of CBDA-treated cells with respect to untreated control.

LDH Cytotoxicity Assay
Lactate dehydrogenase (LDH) activity was measured by using the Cytotoxicity LDH Assay Kit-WST (Dojindo Laboratories, Munich, Germany), according to the instructions of manufacturer. Briefly, HaCat cells were seeded at 1.5 × 10 4 cells per well in 96 well plates. Twenty-four hours after seeding, cells were treated with CBDA at 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 25, 50 and 100 µM (final concentration level in the volume of 100 µL), in the medium for 24 h exposure time (12 wells for each treatment). Untreated HaCaT cells were also incubated (12 wells). After incubation times, a high control was established by adding 10 µL of the lysis buffer to 6 wells for each treatment and to 6 wells of untreated cells. The plate was incubated at 37 • C for 30 min in the CO 2 incubator, and then the working solution (100 µL) was added. The mixtures reacted for other 30 min, after that the stop solution (50 µL) was added, and the absorbance was read at 490 nm by using a Tecan SpectraFluor fluorescence and absorbance reader. The LDH cytotoxicity was expressed using the following equation: ((test compound − low control)/(high control − low control)) × 100 [57].

Cytokine Analysis
HaCat cells were seeded at 3.0 × 10 5 cells per well in 6-well plates. After 24 h, cells were treated with CBDA at 2.5, 5, and 10 µM concentration levels, in free-serum medium, for 48 h exposure time. Following cell harvesting, supernatants were collected and stored at −80 • C until use. Cytokines, chemokines and growth factors levels were evaluated using a Bio-Plex MAGPIX Multiplex Reader system (Bio-Rad, Milan, Italy) equipped with a Bio-Plex Manager software v 6.1 (BioRad) according to manufacturer's instructions [58]. All washing steps were performed on the Bio-Plex magnetic wash station (BioRad). Measurements were performed in triplicate on samples (50 µL) using Bio-Plex Pro™ Human Cytokine 27-plex Assay (Bio-Rad). Standard curves optimization and the calculation of analyte concentrations were performed by using the Bio-Plex Manager software. Data were expressed as mean ± SD.

Bacterial Strains and Growth Conditions
CBDA was tested against Escherichia coli ATCC 13762, Staphylococcus aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. All bacterial strains were cultured on BD Brain Heart Infusion broth (BHI) (BD, Franklin Lakes, NJ, USA) or on BHI agar (OXOID, Basingstoke, Hampshire, England). The microorganisms were stored at −80 • C in BHI broth containing 10% glycerol (v/v) (Carlo Erba, Reagents, Milan, Italy) until use and the working cultures were activated in the respective broth at 37 • C with shaking at 225 rpm for 15-18 h. Overnight cultures were diluted to the required cell concentration (≈10 6 CFU/mL) in BHI broth for growth experiments.

Antibacterial Activity Assay-MIC and MBC Determination
The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of CBDA were determined with a modified version of the broth microdilution assay of the Clinical and Laboratory Standards Institute using a final inoculum concentration of 10 5 CFU/mL. Briefly, overnight cultures were diluted in fresh broth and allowed to grow until an optical density at 600 nm (OD600nm) of 0.

In Vitro Time-Killing Assay
The kinetics of CBDA antimicrobial activity against all ATCC bacterial strains was assessed at CBDA concentrations corresponding to MIC, 2X MIC, 4X MIC. For time killing studies, the bacterial strains were grown in BHI broth overnight at 37 • C, diluted and sub-cultured until late-log phase containing approximately 10 6 CFU/mL (adjusted by spectrophotometer to OD600nm) and were centrifuged at 3.000 rpm for 10 min. The pellets were washed and diluted 1:100 in PBS 1X and then incubated at 37 • C with and without CBDA. An aliquot of each sample was taken at baseline and after 1, 2, 3, and 5 h of incubation; subsequently serially diluted in PBS 1X and seeded on BHI agar [59]. The plates were incubated for 24 h at 37 • C and viable bacterial counts were performed by the CFU method [59]. Time-kill curves were plotted graphically as log 10 CFU/mL vs. time. Each assay was performed in triplicate and all data were expressed as the mean ± SD of three individual experiments.

Biofilm Formation Assay
Biofilm formation assay of E. coli ATCC 13762, S. aureus ATCC 6538P, P. aeruginosa ATCC 27853 and E. faecalis ATCC 29212 was conducted in 96-well plates. Bacteria from overnight cultures were diluted and growth until 0.5 McFarland. The bacteria were diluted 1:100 and plated in each well containing 200 µL of the BHI broth. To evaluate the impact of the CBDA on biofilm formation, the medium was supplemented with different concentrations of cannabis extract equal to MIC, 2X MIC, 4X MIC and incubated overnight. At the end of the incubation, the medium was removed; the biofilms were washed twice with PBS and colored with crystal violet (1%) for 30 min, then resuspended in 200 µL of absolute ethanol. Optical density measurements were performed with a spectrophotometer (Enspire, Perkin Elmer, Winter Street, Waltham, MA, USA) at 595 nm. Bacteria incubated with medium alone were used as negative controls, while positive controls were bacteria incubated in medium supplemented with gentamicin 0.2 µg/mL. S. aureus and P. aeruginosa biofilm formation was assessed also through imaging. After medium was supplemented with different concentrations of CBDA (4.0, 8.0, 16.0 µg/mL for S. aureus and 8.0, 16.0, 32.0 µg/mL for P. aeruginosa) and incubated overnight, biofilms were stained with a Film-Tracer LIVE/DEAD biofilm viability kit (Thermo Fisher Scientific, Third Avenue, Waltham, MA, USA) according to the manufacturer's instructions. Images were acquired by Cell Discoverer 7, Zeiss. Each assay was performed in triplicate and all data were expressed as the mean ± SD of three individual experiments.

SYTOX Green Uptake Assay Using Fluorescent Spectroscopy
The effect of CBDA on the S. aureus ATCC 6538 and E. coli ATCC 13762 membrane integrity was assessed by measuring the extent of intracellular accumulation of SYTOX green. Cells from mid-log-phase were collected, washed and resuspended in 10 mM phosphate buffer. The final density was adjusted to 5 × 10 7 CFU/mL. Cells were then treated with CBDA at concentrations equal to MIC, 2X MIC, 4X MIC (4.0, 8.0, 16.0 µg/mL for S. aureus and 0.5, 1.0, 2.0 µg/mL for E. coli) in presence of 200 nM SYTOX green (Invitrogen, Waltham, MA, USA). Enhancement in SYTOX green fluorescence, a direct measure of the extent of membrane permeabilization, was monitored in a fluorescence spectrophotometer (Enspire, Perkin Elmer). Excitation and emission wavelengths used were 503 and 523 nm, respectively.

Conclusions
In spite of a not so glorious past, Cannabidiolic acid is a bioactive compound worthy of attention in the industrial hemp nutraceutical and cosmeceutical scenario. Edible hemp oil benefits from the presence of this compound, which, together with other nutritionally valuable compounds, makes this oil a precious functional product. On the other hand, the possibility of recovering the compound from hemp food chain waste opens up new insights into the exploitation of its anti-inflammatory and antimicrobial power for skin health.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules27082566/s1, Figure S1. TOF-MS and MS/MS spectra of CBDA detected in leaf hemp sample and cold-pressed hemp seed oil. Red arrows highlight the corresponding peaks in the TICs (Total Ion Chromatograms). Figure S2. UHPLC-TOF-MS chromatogram of purified CBDA, acquired using the same experimental conditions of hemp leaf extract. Figure S3