Secondary Metabolites, including a New 5,6-Dihydropyran-2-One, Produced by the Fungus Diplodia corticola. Aphicidal Activity of the Main Metabolite, Sphaeropsidin A

An undescribed 5,6-dihydropyran-2-one, namely diplopyrone C, was isolated and characterized from the cultures of an isolate of the fungus Diplodia corticola recovered from Quercus suber in Algeria. The structure and relative stereostructure of (5S,6S,7Z,9S,10S)-5-hydroxy-6-(2-(3-methyloxiran-2-yl)vinyl)-5,6-dihydro-2H-pyran-2-one were assigned essentially based on NMR and MS data. Furthermore, ten known compounds were isolated and identified in the same cultures. The most abundant product, the tetracyclic pimarane diterpene sphaeropsidin A, was tested for insecticidal effects against the model sucking aphid, Acyrthosiphon pisum. Results showed a toxic dose-dependent oral activity of sphaeropsidin A, with an LC50 of 9.64 mM.


Introduction
Diplodia (Dothideomycetes, Botryosphaeriaceae) is a widely diffused genus of fungi with more than 1000 described species [1]. Over the years, these species have been reported as pathogens or endophytes of many woody plants [1,2]. Moreover, they represent a prolific source of bioactive products with huge structural variability and bioactivities [3][4][5][6]. In this respect, the capacity of Diplodia spp. to have distinct habitus and interactions with plants may be related to the release of bioactive compounds during the spread in host tissues.
Among the Diplodia species, Diplodia corticola A.J.L. Phillips, A. Alves, and J. Luque is particularly regarded for the production of secondary metabolites [7][8][9][10]. It is frequently associated with dieback and canker diseases of oaks in many Mediterranean countries [11][12][13]. Among the metabolites frequently isolated from in vitro cultures of D. corticola, sphaeropsidin A is particularly promising for practical applications in agriculture and medicine due to its exciting biological properties, including antimicrobial, insecticidal, herbicidal, and anticancer activities [14]. Besides potential applications, the documented antimicrobial and insecticidal effects of sphaeropsidin A [15][16][17][18] are relevant for further consideration of the ecological role of the fungus.
In this work, a strain of D. corticola isolated from Quercus suber in Algeria was investigated in order to increase the available data on the secondary metabolism of this fungus, leading to the isolation of a new 5,6-dihydropyran-2-one, namely diplopyrone C, and ten known compounds which include sphaeropsidin A. Following documented evidence of insecticidal properties [15,18], sphaeropsidin A was tested for aphicidal activity on the pea aphid Acyrthosiphon pisum (Harris) (Hemiptera, Aphididae), which is a cosmopolitan polyphagous insect and one of the primary species used as laboratory models for testing the susceptibility of sucking insects to oral administration of insecticidal products by using a feeding bioassay on an artificial diet.
Molecules 2022, 27, x FOR PEER REVIEW 2 of 10 medicine due to its exciting biological properties, including antimicrobial, insecticidal, herbicidal, and anticancer activities [14]. Besides potential applications, the documented antimicrobial and insecticidal effects of sphaeropsidin A [15][16][17][18] are relevant for further consideration of the ecological role of the fungus. In this work, a strain of D. corticola isolated from Quercus suber in Algeria was investigated in order to increase the available data on the secondary metabolism of this fungus, leading to the isolation of a new 5,6-dihydropyran-2-one, namely diplopyrone C, and ten known compounds which include sphaeropsidin A. Following documented evidence of insecticidal properties [15,18], sphaeropsidin A was tested for aphicidal activity on the pea aphid Acyrthosiphon pisum (Harris) (Hemiptera, Aphididae), which is a cosmopolitan polyphagous insect and one of the primary species used as laboratory models for testing the susceptibility of sucking insects to oral administration of insecticidal products by using a feeding bioassay on an artificial diet.

Oral Toxicity of Sphaeropsidin A on Acyrthosiphon pisum
Sphaeropsidin A showed an oral lethal activity on aphids at all the doses tested, and the resulting survival rate was significantly lower compared to the control (log-rank test: ꭕ 2 = 561.1, p < 0.0001, dF = 4). The highest doses induced mortality starting from day 2. The mortality increased over time in a dose-dependent manner, and no aphids survived after 7 days of administration ( Figure 3). The Z configuration of the double bond at C-7-C-8 was assigned on the basis of the typical coupling constant (11.7 Hz) [27]. The relative configuration of compound 1 was assigned on the basis of the NOE effects observed in the NOESY spectrum ( Figure S6). The NOE effect of H-7 with H-8 confirmed the Z configuration of the chain double bond. The NOE effect of H-5 with H-6 and the coupling constant of 3.3 Hz indicated a cis configuration of the hydroxyl group and the side chain of the dihydropyrone ring. The absence of the NOE effect between the H-9 and the H-10 and the NOE effect of the H-11 methyl with the H-9 indicated a trans configuration of the oxirane ring ( Figure 2). All spectral data allowed to the determine the structure and relative stereostructure of compound 1 as (5S,6S,7Z,9S,10S)-5-hydroxy-6-(2-(3-methyloxiran-2-yl)vinyl)-5,6-dihydro-2H-pyran-2one, named diplopyrone C.

Oral Toxicity of Sphaeropsidin A on Acyrthosiphon pisum
Sphaeropsidin A showed an oral lethal activity on aphids at all the doses tested, and the resulting survival rate was significantly lower compared to the control (log-rank test: χ 2 = 561.1, p < 0.0001, dF = 4). The highest doses induced mortality starting from day 2. The mortality increased over time in a dose-dependent manner, and no aphids survived after 7 days of administration ( Figure 3).

Oral Toxicity of Sphaeropsidin A on Acyrthosiphon pisum
Sphaeropsidin A showed an oral lethal activity on aphids at all the doses tested, and the resulting survival rate was significantly lower compared to the control (log-rank test: ꭕ 2 = 561.1, p < 0.0001, dF = 4). The highest doses induced mortality starting from day 2. The mortality increased over time in a dose-dependent manner, and no aphids survived after 7 days of administration ( Figure 3). The lethal concentrations of sphaeropsidin A, resulting in 10%, 50%, and 90% mortality of the pea aphids (LC10, LC50, and LC90, respectively) and 95% confidence intervals, were determined on day 6 and showed that LC10 (95% CI) is 4.5 mM (4.12-4.93), LC50 (95% CI) is 9.64 mM (9.23-10.06), and LC90 is 20.43 mM (18.81-21.57). The lethal activity of sphaeropsidin A on day 6 is shown in Figure 4. The lethal concentrations of sphaeropsidin A, resulting in 10%, 50%, and 90% mortality of the pea aphids (LC 10 , LC 50 , and LC 90 , respectively) and 95% confidence intervals, were determined on day 6 and showed that LC 10 (95% CI) is 4.5 mM (4.12-4.93), LC 50 (95% CI) is 9.64 mM (9.23-10.06), and LC 90 is 20.43 mM (18.81-21.57). The lethal activity of sphaeropsidin A on day 6 is shown in Figure 4.

Oral Toxicity of Sphaeropsidin A on Acyrthosiphon pisum
Sphaeropsidin A showed an oral lethal activity on aphids at all the doses tested, and the resulting survival rate was significantly lower compared to the control (log-rank test: ꭕ 2 = 561.1, p < 0.0001, dF = 4). The highest doses induced mortality starting from day 2. The mortality increased over time in a dose-dependent manner, and no aphids survived after 7 days of administration (Figure 3). The lethal concentrations of sphaeropsidin A, resulting in 10%, 50%, and 90% mortality of the pea aphids (LC10, LC50, and LC90, respectively) and 95% confidence intervals, were determined on day 6 and showed that LC10 (95% CI) is 4.5 mM (4.12-4.93), LC50 (95% CI) is 9.64 mM (9.23-10.06), and LC90 is 20.43 mM (18.81-21.57). The lethal activity of sphaeropsidin A on day 6 is shown in Figure 4.

Discussion
Secondary metabolites are often used by microbes to enable unique trophic lifestyles, overcome competition with other microbes, or cope with environmental biotic and abiotic stress [28][29][30][31]. Hence, in this study, the production of secondary metabolites from a strain of D. corticola was examined with reference to its possible ecological role.
The high production rate of sphaeropsidin A suggests its possible involvement in the dynamic interaction with the host plant; in this regard, the anti-insectan properties deserve particular attention in regard to the hypothesis that this compound is also released in vivo. In fact, a widespread anti-insectan effect is corroborated by results of previous studies showing fagodeterrent and larvicidal activity against the mosquito Aedes aegypti (Diptera, Culicidae) [15] and oral toxic activity against larvae of the chewing model insect Spodoptera littoralis (Lepidoptera, Noctuidae) [18].
Here we have demonstrated the possible effects of sphaeropsidin A on sucking insects based on a dose-dependent toxic oral activity against the model phloem sucking insect, A. pisum. Hence, if confirmed in planta, production of this secondary metabolite may reduce the impact of herbivorous insects, representing an indication of defensive mutualism established during the development of this fungus as an endophyte or latent pathogen.
Moreover, the oral toxic activity shown by sphaeropsidin A on pea aphids stimulates further investigation of its mode of action from the perspective of its possible application as a new pesticide that meets the growing demand for alternative products with low environmental impact.

General Experimental Procedures
The optical rotations of pure metabolites were measured in CHCl 3 or MeOH on a Jasco P-1010 digital polarimeter (Tokyo, Japan). FT-IR spectra were recorded in modality ATR (attenuated total reflectance) with model Nicolet 5700 by Thermo Electric Corporation (Waltham, MA, USA). The measuring cell consisted of a mono crystal of zinc selenide. The blank was recorded using air as reference. UV spectra were recorded in CH 3 CN by Cary model 5000 Spectrophotometer by Varian C. (Palo Alto, CA, USA). 1 H and 13 C NMR spectra were recorded on a Bruker AMX instrument at 400 and 100 MHz, respectively, in CDCl 3 . The same solvents were used as internal standards. COSY-45, HSQC, HMBC, and NOESY were performed using standard Bruker microprograms. TLC was performed on silica gel (Kieselgel 60, F 254 , 0.25 mm, Merck, Darmstadt, Germany) or reverse-phase plates (Whatman, KC18 F 254 , 0.20 mm). The spots were visualized by exposure to UV radiation (253 nm) or by spraying first with 10% H 2 SO 4 in methanol followed by heating at 110 • C for 10 min. Chromatography was performed on silica gel column (Merck, Kieselgel 60, 0.063-0.200 mm). HRESI-TOF mass spectra were measured on an Agilent Technologies ESI-TOF 6230DA instrument in the positive ion mode (Milan, Italy).

Fungal Strain and Cultures Production
Diplodia corticola strain (B305) employed in this study was previously isolated from Q. suber trees showing canker and dieback symptoms in Algeria. The strain was identified and characterized as a pathogen in a previous work [11] based on the integration of morphological features and phylogenetic analysis of the combined ITS and tef1-α sequence data. The nucleotide sequences of D. corticola are available in GenBank database under accession numbers MT015626 and MT066136.
Liquid cultures of the strain were prepared in Czapek-Dox broth (Oxoid, Thermo Scientific, Waltham, MA, USA) amended with 2% cornmeal in 500 mL Erlenmeyer flasks containing 250 mL of the substrate [44] and grown in a stationary phase in the dark at 25 • C for 30 days.

Extraction and Purification Processes of Metabolites 1-11
The culture broth and mycelia were homogenized in a mixer with 350 mL of MeOH (1% NaCl). Then, the suspension was centrifuged for 40 min at 7000 rpm and 10 • C. The pellet was resuspended in 150 mL of a mixture of H 2 O:MeOH (45:55 v/v, 1% NaCl) and submitted to a second homogenization followed by centrifugation. Supernatants were collected, and MeOH was evaporated under reduced pressure to obtain an aqueous solution for the subsequent extraction (3 times) with ethyl acetate at native pH (=6.0). The organic phases were combined, dried with anhydrous Na 2 SO 4 , and evaporated under reduced pressure, yielding crude extract as brown oil (156.7 mg). The organic extract was purified by column chromatography (CC) on silica gel (40 cm

Insects Rearing and Oral Toxicity Bioassay
Acyrthosiphon pisum was reared on potted broad bean plants (Vicia faba) at 20 ± 1 • C, 75 ± 5% RH, and under a 16:8 h light:dark photoperiod, starting with insects originally collected from alfalfa plants in Eboli, southern Italy. In order to synchronize the aphid population, parthenogenetic adult females were placed on plants for 6 h, resulting in neonate nymphs with an age of 0-6 h that were used throughout the experiments.
The oral toxicity of sphaeropsidin A (2) on A. pisum was investigated using a standard basal diet previously developed for assays of test compounds [45]. The feeding system for the pea aphid was realized as described in [45] with minor modifications. Each experimental unit was a feeding system with 10 aphids; four replications per treatment were realized, each replicate consisting of three experimental units per treatment. A total of 120 aphids per treatment were used. In each feeding system, 300 µL of artificial diet containing 1 3.6 mM, 7.2 mM, 14.4 mM, and 28.8 mM was dispensed; negative control was realized using the artificial diet only. The experiment was carried out under the rearing conditions described above. Briefly, on day 0, neonate nymphs were transferred to a freshly prepared diet sachet feeding apparatus. Mortality was recorded daily for one week, and dead nymphs were removed. The artificial diet was replaced every two days. The lethal concentrations of 1 resulting in 10%, 50%, and 90% aphid mortality (defined as LC 10 , LC 50 , and LC 90 ) and the corresponding 95% confidence intervals were determined.

Statistical Analysis
Aphid survival curves were compared using Kaplan-Meier and log-rank analysis. The results obtained were analyzed using non-linear sigmoid curve fitting, and the activity of each treatment was evaluated on day 6 on the basis of dose-response concentrations; the goodness of fit to the curve model was evaluated on the basis of R 2 values. Data were analyzed using Prism 6 (GraphPad Software Inc. version 6.0b, San Diego, CA, USA).

Data Availability Statement:
The data that support the findings of this study are available from the corresponding author upon reasonable request.

Conflicts of Interest:
The authors declare no conflict of interest.