Sulforaphane and Its Bifunctional Analogs: Synthesis and Biological Activity

For decades, various plants have been studied as sources of biologically active compounds. Compounds with anticancer and antimicrobial properties are the most frequently desired. Cruciferous plants, including Brussels sprouts, broccoli, and wasabi, have a special role in the research studies. Studies have shown that consumption of these plants reduce the risk of lung, breast, and prostate cancers. The high chemopreventive and anticancer potential of cruciferous plants results from the presence of a large amount of glucosinolates, which, under the influence of myrosinase, undergo an enzymatic transformation to biologically active isothiocyanates (ITCs). Natural isothiocyanates, such as benzyl isothiocyanate, phenethyl isothiocyanate, or the best-tested sulforaphane, possess anticancer activity at all stages of the carcinogenesis process, show antibacterial activity, and are used in organic synthesis. Methods of synthesis of sulforaphane, as well as its natural or synthetic bifunctional analogues with sulfinyl, sulfanyl, sulfonyl, phosphonate, phosphinate, phosphine oxide, carbonyl, ester, carboxamide, ether, or additional isothiocyanate functional groups, and with the unbranched alkyl chain containing 2–6 carbon atoms, are discussed in this review. The biological activity of these compounds are also reported. In the first section, glucosinolates, isothiocyanates, and mercapturic acids (their metabolites) are briefly characterized. Additionally, the most studied anticancer and antibacterial mechanisms of ITC actions are discussed.


Isothiocyanates
Biologically inactive GSLs undergo enzymatic transformations to biologically active isothiocyanates (ITCs). ITCs [34][35][36][37] are low molecular weight heterocummulenes with a general formula R-N=C=S (R-NCS), where R may be a structurally diverse aliphatic, aromatic or heterocyclic substituent. They are characterized by sharp and pungency odors [38] and relatively high volatility. Due to the presence of a reactive electrophilic carbon atom in the isothiocyanate group (-NCS), these compounds easily react in physiological conditions, in a reversible way with thiols, resulting in sensitivity to pH dithiocarbamates or forming thioureas in an irreversible reaction with amines. It was shown that ITCs react 1000 times faster with thiols than with amines [39]. Hydrolysis of ITCs leads to amines ( Figure 3) [40].

The Mechanism of Anticancer Activity
There are many excellent reviews on anticancer activities, as well as the mechanisms of action of ITCs [92][93][94][95][96] and SFN [97][98][99][100]. In vitro and in vivo tested ITCs, including SFN, show their biological activities at all stages of carcinogenesis. Generally, carcinogenesis consists of three stages: (i) initiation-a rapid and irreversible process leading to genotypic changes and DNA damage due to interaction with the carcinogen; (ii) promotion-the selective proliferation of initiated cells leading to the formation of pre-cancerous lesions; (iii) progression-transformation of benign pre-cancerous lesions into cancer. This section briefly summarizes the most studied ITC anticancer mechanisms.

Initiation Stage
At the initiation stage, isothiocyanates exhibit chemopreventive activities by regulating xenobiotics metabolism. In the conversion of carcinogens and their elimination, two groups of enzymes participate: phase I enzymes and phase II enzymes.
In phase II metabolism of carcinogens, a particular role is played by glutathione Stransferase (GST), NAD(P)H: quinone oxidoreductase (NQO1), UDP-glucuronyltransferase (UGT), quinone reductase (QR), and nicotinamide N-methyltransferase [103,104], enzymes responsible for increase the solubility of xenobiotics in water and facilitate their excretion [105]. In vitro studies on the HepG2 human hepatocyte line showed that the use of SFN (24) caused an increase in the concentration of mRNA UGT 1A1 and GST A1 [106], and an increase in the activity of NQO1 [107], accompanied by bilirubin glucuronidation [108].
One of the proposed mechanisms of stimulation of phase II enzymes by SFN concerns the activation of the Nrf2 [109] allowing the interaction with the antioxidant response element (ARE) encoding the detoxification enzyme genes. Under normal conditions, Nrf2 occurs in the cellular cytosol, and it is binding to the Kelch-like ECH-associated protein 1 (Keap1) leading the Nrf2-Keap1 complex. SFN, or other ITCs after entering the cell, react with thiol groups present on the surface of the Keap1, resulting in the degradation of the Nrf2-Keap1 complex. The released and activated Nrf2 migrates to the cell nucleus, where it binds to ARE and stimulates transcription of genes encoding phase II enzymes [109]. Studies have shown that the activation of Nrf2, apart from what is described above, can also take place due to the activation of the MAPK protein kinase pathway [46].

Promotion Stage
At the promotion stage, SFN (and other ITCs) show antiproliferative activity against tumor cells by, e.g., inhibiting the cell cycle, inducing apoptosis or inhibiting histone deacetylase (HDAC).

Inhibition of the Cell Cycle
Tumor cells are characterized by rapid growth resulting from the loss or disturbed functioning of mechanisms of cell cycle regulation. Cyclin-dependent kinases (CDKs), cyclins, and cyclin-dependent kinase inhibitors are involved in controlling the cycle of healthy cells. The formation of a cyclin and CDK complexes permit the cell to pass through the next phases of the cycle, while the CDK inhibitors stop the cycle in a specific phase. The research has shown that SFN acts as a cell cycle regulator, inhibiting the cell cycle in the G2/M phase [110]. Crossing the G2/M point requires activation of a complex of the cyclin B and CDK1. Phosphorylation of CDK1 by Wee1 and Myt1 kinases leads to inactivation of the complex, while the activity of the Cdc25 protein phosphatase conditions the activity of the complex, allowing to go to the M phase. The Cdc25 protein phosphatase is regulated by Chk1 and Chk2 kinases, and phosphorylation of Cdc25 inactivates it and leads to the deactivation of the cyclin B and CDK1 complex, finally stopping the cell cycle in the G2/M phase [111]. Studies have shown that SFN prevents the transfer of PC-3 cancer cells to the M phase of the cell cycle by decreasing the levels of cyclin B1, Cdk25B, and Cdk25C, and increasing phosphorylation of Cdk25C by Chk2 [112]. In addition, it was shown that one of the mechanisms of action of SFN on the HT-29 colorectal tumor cell line is the expression of protein p21 [113]. Studies on the same cell line have shown that ITCs also inhibits the cell cycle in the G1 phase by reducing regulation of cyclin A, D, and E [114]. The apoptotic effect depends mainly on the dose of the compound and the time of exposure to it [115]. Studies on the Caco-2 cell line have shown that SFN at a concentration of 20 µM inhibits the cell cycle in the G2/M phase, while at a concentration above 20 µM, it inhibits the G1 phase. In addition, the short-term exposure of tumor cells to SFN resulted in reversible inhibition of the cell cycle in the G2/M phase, while complete inhibition of the cell cycle required more than 12 h of incubation [116].

Inducing Apoptosis
Apoptosis, programmed cell death, is one of the most commonly used strategies to fight cancer cells. In some cases, the low susceptibility of cancer cells to signals triggering the process of apoptosis contributes to the development of tumors. SFN is involved in activating signals that lead to apoptosis in many types of cancer cells. SFN stimulates the formation of apoptotic bodies, reduces the concentration of anti-apoptotic Bcl-2 and Bcl-XL proteins, increases the expression of the proapoptotic Bax protein, the activation of caspase 3, and the degradation of the poly(ADP-ribose) polymerase, and thus interacts on the mitochondrial-dependent apoptosis factors [117]. In addition, SFN is responsible for decreasing the activity of apoptosis inhibitors (IAP: cIAP1, cIAP2, and XIAP) and the induction of the Apaf-1 protein [118]. In addition to SFN, other natural isothiocyanates participate in the process of apoptosis. BITC activates procaspases-8 and -9 [119]. AITC and PEITC increase the level of the t-Bid proapoptotic protein in HL-60 leukemia cells [120], and AITC further reduces the Bcl-XL protein concentration in LNCaP prostate cancer cells [121]. Mechanistic studies suggest that the generation of the reactive oxygen species (ROS) by SFN induces the mechanism of cancer cell death. Tests performed on the human prostate tumor cell PC-3 line showed that ROS generated in the presence of SFN changed the potential of the mitochondrial membrane, leading to the release of cytochrome C from the intermembrane space to the cytoplasm [122]. Cytochrome C, together with the Apaf-1 protein and ATP, form an apoptosome, activating procaspase-9. The apoptosome and caspase-9 complex recruits and activates procaspase-3 and/or procaspase-7, leading to apoptosis [123].

Inhibiting Histone Deacetylase (HDAC)
Histones undergo reversible acetylation of selected N-terminus lysine residues. The modification occurring in the presence of acetyltransferase leads to serious changes at all levels of chromatin structures, causing disorders of the chromatin, folding into higherorder structures [124], increasing its solubility under physiological conditions and, most importantly, favor transcription [125]. Unlike acetylation, deacetylation of histones leads to the blockage of chromatin structures and, as a consequence, to the termination of the transcription process. Histone deacetylase is associated with many cancers, it causes suppression of transcription and affects the dysregulation of mechanisms controlling the cell cycle and apoptosis. In addition, HDAC by deacetylation of tumor suppressor genes, e.g., the p21 gene, leads to silencing of their transcriptions or their complete deactivation. In vitro studies performed on the HCT 116 cell line showed that SFN at concentrations of 3-15 µM causes a decrease in HDAC activity [126]. The process of inhibiting histone deacetylase is combined with the inhibition of the cell cycle and induction of apoptosis. Studies show that inhibition of HDAC with SFN contribute to inhibition of the cell cycle in PC-3 cells in the G2/M phase [127], while inhibition of HDAC using BITC causes the deactivation of NF-κB, which leads to decreased activity of cyclin D1 and, consequently, to the inhibition of the cell cycle [128]. In addition, as a consequence of the inhibition of histone deacetylase with SFN, researchers observed an increase in the concentration of p21 and Bax proteins [126], which are involved in the process of apoptosis.

Progression Stage
At the stage of progression, isothiocyanates, as well as SFN, are responsible for inhibiting the process of angiogenesis and metastasis.

Inhibition of Angiogenesis and Metastasis
Angiogenesis is the process of formation of new blood vessels [129]. It is claimed that it is an important stage in tumor growth and metastasis, as a result of which, oxygen and nutrients are supplied to the formed cancer. For this reason, the stage of angiogenesis has become the target of anticancer therapies. The main cytokinin initiating the process of angiogenesis is the vascular endothelial growth factor (VEGF), responsible for an increase in vascular permeability and stimulating proteolytic enzymes. In addition to VEGF, fibroblast growth factor 2 (FGF-2) and epidermal growth factor (EGF) are important factors involved in angiogenesis [130].
Metastasis is the ability to spread cancer cells from the primary outbreak to lymph nodes, and to tissues and organs, and is a hallmark of malignant tumors. The metastasis process requires the activation of proteolytic enzymes, e.g., metalloproteinases (MMPs). These enzymes belong to the family of zinc-containing enzymes and are capable of degrading the basement membrane, which is necessary to penetrate endothelial cells into new places and create new vessels. MMPs are overexpressed in cancer cells [131]. Studies have shown that PEITC has an anti-angiogenic effect by inhibiting the activity of VEGF and EGF [132]. Additionally, tests on human umbilical vein endothelial cells (HUVECs) with SFN have demonstrated that this compound is involved in the regulation of the various stages of angiogenesis, by reducing vascular formation and propagation of endothelial cells [133]. In addition, SFN inhibits metalloproteinase-9 activity and reduces the metastatic ability of MDA-MB-231, a triple-negative breast cancer cell line.

Antibacterial Activity
Antibacterial properties of ITCs are not as wide tested as anticancer properties. To date, only two extensive reviews have described the antibacterial activities of ITCs. Dufour et al. [53] in 2015 described, in detail, the antibacterial modes of action, e.g., the effects on influencing the membrane, inhibition of enzymic or regulatory activities, the effect of ITCs on respiratory enzymes, the induction of heat shock and oxidative stress responses, and the induction of a stringent response of natural ITCs (SFN, BITC, PEITC, etc.), as well as mechanisms of resistance to ITCs. Moreover, Romeo et al. [54], in 2018, described the antibacterial properties of natural ITCs against Gram-positive (H. pylori, S. aureus, etc.) and Gram-negative (P. aeruginosa, E. coli, etc.) bacteria. For this reason, in this review, the antibacterial activities of ITCs are generally and briefly characterized.
In Japan, ITCs are used as natural food additives to protect them against microorganisms [134]. Highly volatile allyl isothiocyanate (AITC) plays a special role. It is used in antimicrobial food packaging to reduce, inhibit, and delay the growth of microorganisms in packed food. ITCs obtained from cruciferous vegetables, most often from horseradish, wasabi, or radish, are also added to food as spices [135]. The bacteriostatic and bactericidal effect of ITCs depend on the dose, and the concentration responsible for the abovementioned effects is comparable or lower than the concentration of classical antibiotics used for the same purposes. ITCs show synergism with commonly used antibiotics. For example, a solution of p-hydroxyphenethyl isothiocyanate in glucose exhibits synergism with aminoglycoside antibiotics and streptomycin in relation to E. coli and S. aureus [136]. Moreover, AITC and phenethyl isothiocyanate (PEITC) show synergism with streptomycin against some Gram-negative bacteria (E. coli or P. aeruginosa) [137]. However, it was shown that even a small change in the concentration of isothiocyanate or antibiotic can cause the opposite effect and suppress antibacterial effect [138]. The mechanism of synergism, or its quenching caused by ITCs, is not yet known. Bacteriostatic properties of ITCs have also been noticed in agriculture, and ITCs are used to reduce the population of bacteria found in soil; for this purpose-genetically modified Arabidopsis thaliana (radish) plants using a transgenic A. thaliana that overexpress glucosinolate with p-hydroxybenzyl substituent (14, sinalbin, Figure 1) [139]. Glucosinolates, by diffusion, are transported from the roots to the rhizosphere, where under the action of extracellular myrosinase, they are transformed into ITCs [140].

Mechanism of Antibacterial Activity
The mechanism of antibacterial activity of ITCs is not as well understood. It is claimed that the antibacterial activity of ITCs is associated with disintegration of the cell membrane (causing the outflow of all metabolites), inhibition of bacterial quorum sensing-a system of communication between bacteria through autoinducers, inhibition of biofilm production, inhibition of enzymes necessary for the proper functioning of bacteria, induction of thermal shock, or induction of oxidative stress. The most commonly studied Gram-negative and Gram-positive bacteria strains and the antibacterial mechanisms of ITCs are briefly discussed in this section.
Regarding H. pylori (Gram-negative) [141], SFN has the highest activity in relation to this strain, as well as to resistant strains of H. pylori (an inhibition of SFN is similar to the inhibition of antibiotics-clarithromycin and metronidazole) [142]. It is known that stomach infections by H. pylori are possible due to the ureases [143] converting urea into ammonia, resulting in the neutralization of acidic gastric juices [144]. SFN and other ITCs likely inhibit the activity of H. pylori urease, but this is still the subject of many studies [145].
Regarding Ca. jejuni (Gram-negative), benzyl isothiocyanate (BITC) is the most active natural isothiocyanate in relation to both antibiotic-resistant and -sensitive strains, Ca. jejuni [146]. Studies show that antibacterial activity of BITC is associated with the activation of metabolic pathways responsible for thermal shock and oxidative stress, leading to protein aggregation, energy metabolism disorders and, finally, bacterial death [147].
Enterohemorrhagic E. coli (EHEC) (Gram-negative) [150], whose strain O157:H7 produces the Shiga toxin, is the most studied, enteric, pathogenic E. coli strain. AITC is characterized by antibacterial activity on the E. coli O157:H7 strain, similar to polymyxin B, causing cell membrane disintegration and metabolite efflux and, consequently, bacterial death [151]. AITC inhibits two key enzymes in the metabolism of bacteria: thioredoxin reductase, involved in the synthesis of ribonucleotides, and acetate kinase, which is associated with energy metabolism [152]. From the group of natural ITCs (SFN, AITC, BITC, phenyl, and isopropyl isothiocyanate), the highest comparable to AITC activity in inhibiting E. coli EHEC, including E. coli O157:H7, showed BITC. In comparison to conventional antibiotics, the tested ITCs better inhibited the production of the Shiga toxin. Detailed studies have shown that the aforementioned ITCs affect penta/tetraphosphate (p)ppGpp, which influences RNA polymerase activity, bacteriophages development, and Shiga toxin production. ITCs increase the levels of penta/tetraphosphate (p)ppGpp, decrease the synthesis of RNA, and inhibit the development of prophageand the production of the Shiga toxin [153].
P. aeruginosa (Gram-negative) aerobic bacterium is able to colonize various environments and produce biofilm [154,155]. Many bacteria, including Pseudomonas strains, are equipped with a quorum sensing (QS) [156] system, by which bacteria communicate to coordinate gene expression. This allows them to control the expression of genes that are important for the entire population, by secreting and receiving signal molecules called autoinducers. This system controls biofilm formation, bioluminescence generation, the production of antibiotics and siderophores, and bacterial motility [157]. These coordinated behaviors allow bacteria to compete with multicellular organisms and survive sharp and sudden environmental changes. In P. aeruginosa, there are two main QS systems-las and rhl.
The las system consists of synthesis LasI, the "autoinduction" gene responsible for the synthesis of autoinducers N-[3-oxo-dodecanoyl]-L-homoserine lactones (3-oxo-C12-HSL) and lasR, genes encoding transcription regulators. The rhl system consists of the pair RhlI/RhlR, which respond to N-butyryl homoserine lactones (C 4 -HSL) [158][159][160]. Studies on natural isothiocyanates (AITC, BITC, and PEITC) and the mixtures of those ITCs have shown that AITC and the "cocktail" of ITCs were characterized by the highest activity. However, only PEITC inhibited biofilm production [161]. In other studies, AITC and PEITC caused cell membrane disintegration and an increase in the hydrophilic nature of the membrane, changing its physicochemical properties [162]. Meijler et al. [163], with the P. aeruginosa strain, showed that by using SFN and its sulfide analog erucin (28, Figure 5), it is possible to affect bacterial QS. The consequence of this was the inhibition of the production of biofilm and pyocyanin-a cytotoxic dye that affects the central nervous system, urological system, and vascular system, causing inflammation [164]. Both biofilm and pyocyanin production are virulence factors controlled by QS. Iberin (25, Figure 5), an SFN analogue, also affects bacterial QS, by reducing the activity of the RhlI/RhlR expression [165].
Studies on the activities of natural ITCs (AITC, BITC, PEITC, and SFN) and mixtures of these ITCs (AITC, BITC, and PEITC) have shown that the mixtures show the highest activities in relation to the S. aureus [166,167] (Gram-positive) strain. Among individual ITCs, BITC had the best activity, while AITC was inactive. It was also shown that BITC, PEITC, and SFN, and their mixtures showed higher activity than vancomycin [168]. Contrary to earlier research suggesting that AITC is inactive against S. aureus, Lu et al. [169], in 2016, proved that AITC causes growth inhibition of S. aureus, and similar to PEITC, causes cell membrane disintegration and bacterial death [162].

Clinical Trials of SFN
In addition to in vitro and in vivo tests, SFN has also been selected for clinical trials; however, there is a limited number of these results in the literature. Talalay et al. [170] described a placebo-controlled, double-blind phase I clinical trial of healthy volunteers using extracts of sprouts containing either glucosinolates (principally glucoraphanin, the precursor of SFN) or ITCs (principally SFN). After 7 days of trials, no significant toxicities associated with taking the extracts at the doses employed were observed. In 2007, Visvanathan et al. [171] published research, where eight healthy women took an oral dose of broccoli sprout preparations containing 200 µmol of SFN. Studies demonstrated that sulforaphane distributed to the breast epithelial cells in vivo and exerted pharmacodynamic action in these target cells, consistent with its mechanism of chemoprotective efficacy. Fahey et al. [172] showed that administration of sulforaphane (100 µmol/day on 14 days) improved the bronchoprotection response in asthmatics who had an increase in NQO1 gene expression and did not have a decrease in their initial response to the MCh challenge. Therefore, SFN administration was able to improve a major defect of even mild asthma. Yanaka and co-workers [173] conducted research in which they fed forty-eight H. pyloriinfected patients with broccoli sprouts (70 g/d; containing 420 µmol of SFN precursor) for 8 weeks. Results showed antibacterial effects of SFN on H. pylori, leading to reduced gastritis, as well as an indirect (systemic) effect by increasing the mammalian cytoprotective (phase II) response. More information about the clinical trials of SFN are in the review articles [174,175].
Although SFN was tested in phase I and phase II clinical trials, where it had good anticancer and antibacterial properties, it was never qualified as a drug. This may be due to the polymorphism of the genes for the GSTM1 and GSTT1 glutathione S-transferase isoenzymes. GST is involved in the detoxification of many chemical carcinogens and is responsible for the metabolism of ingested ITCs. Cohort studies indicate a protective effect of a diet rich in cruciferous vegetables against cancers of the lung, colon and breast only in patients with GSTM1 and GSTT1-null genotype [176]. Due to the fact that GST participates in the metabolism of SFN and thus affects its excretion, lower enzyme activity in people with GST gene polymorphism may result in slower elimination and longer exposure of cancer cells to this compound provided with the diet. In people with gene GSTT1 a greater excretion of ITCs outside the body was observed, and thus a shorter time of exposure to cancer cells [177].

Methods of Synthesis of ITCs
Many ITC synthesis methods are described in the literature. The choice of the synthetic method depends on the availability of the starting substrates as well as on the sensitivity of other functional groups present in the substrates to the reaction conditions. Methods on the synthesis of ITCs (known thus far) are described in the Houben-Weyl encyclopedia [178]; new aspects of the synthesis were described by Mukerjee and Ashare [56], Wentrup et al. [179], and Singh et al. [180]. Primary amines are used as starting materials in the synthesis of isothiocyanates ( Figure 8). The synthesis of ITCs from primary amines, or their salts, involve the use of thiocarbonyl reagents, such as thiophosgene (34) and its substitutes, or carbon disulfide (35). They "enable" either directly or indirectly via the intermediate dithiocarbamates transformation of amines into ITCs ( Figure 8, Path a and b). Alternatively, azides may be applied as substrates. These in turn allow the preparation of ITCs via a tandem Staudinger/aza-Wittig reaction, through the intermediate iminophosphoranes ( Figure 8, Path c).

Synthesis ITCs Using Thiophosgene and Its Substitutes
The reaction of primary amines, or their salts with thiophosgene (34), is presently one of the oldest (but most commonly used) methods in the synthesis of ITCs [181,182]. The original product of the reaction is unstable thiocarbonyl chloride derivative [183], which, after elimination of hydrogen chloride, in an alkaline medium is transformed into the target isothiocyanate ( Figure 9). Thiophosgene enables efficient conversion of both aliphatic and aromatic amines into ITCs. This method, however, is not suitable for bifunctional amines with reactive nucleophilic groups in the vicinal position because of subsequent cyclization of the original product.
A small excess of thiophosgene is recommended for use to prevent the formation of symmetrical thioureas as a side product. In contrast, the presence of an organic or inorganic base facilitates the formation of isothiocyanate and serves to neutralize hydrogen chloride. The use of concentrated strong bases (e.g., NaOH) is not recommended in this case because of the easy hydrolysis of ITCs in an alkaline medium. Reactions using thiophosgene can be carried out in a homogeneous-as well as a two-phase-system. In a two-phase system, water-organic solvents, both thiophosgene and the forming ITC, do not undergo subsequent reactions. Chloroform [184] and dichloromethane [185] are the most commonly used organic solvents and, as a base, calcium carbonate [186], sodium bicarbonate [187], or diluted sodium hydroxide solution [188] are usually used. The reaction is carried out at room temperature, and due to the heterogeneity system, intensive stirring is necessary [189]. In a homogeneous system, toluene [190] or acetone [191] are the most often used solvents, and triethylamine is used as a base. Despite the relatively high toxicity of thiophosgene and its unpleasant smell, the aforementioned method is still popular due to its simplicity, versatility, and mild reactive conditions. For these reasons, thiophosgene is sometimes replaced by less toxic and less reactive reagents. Among them, the most widely used are thiocarbonyldiimidazole (36) [192], 1,1 -thiocarbonyldi-2,2 -pyridone (37) [193], and di-2-pyridyl thionocarbonate (38) [194] (Figure 10). Although the use of these reagents guarantees high efficiency of thiocarbonylation, their use increases the cost-effectiveness of the method compared to the thiophosgene method.

Synthesis ITCs with a Desulfurizing Agent
The second most commonly used approach in the synthesis of ITCs is a two-step reaction, involving the conversion of amine or its salt and carbon disulfide (35) into the intermediate dithiocarbamate, which, in the presence of a desulfurizing reagent, undergoes transformation to the target ITCs [195] (Figure 11). Most often, these two-stage reactions are performed in a "one-pot" version. The method is compatible with various functional groups and the range of its applicability is limited only by the nucleophilicity of the starting amines. In most cases, dithiocarbamates are formed quickly and in quantitative yield at room temperature. Only for aromatic amines, with strong electron-withdrawing substituents in the ring, does the formation of dithiocarbamates require the use of strong bases, such as sodium hydride, increasing the reaction time and elevating temperature. The above-mentioned method was described for the first time in 1886 by Hofmann [196]. Since then, there has been rapid development of ITC synthesis using various desulfurizing reagents. Unfortunately, the use of many of them involve drastic reaction conditions; byproducts formed in these reactions, such as heavy metal salts or carbodiimides, are difficult to remove [56]. Therefore, in recent years, effort has been made to find such desulfurizing reagents that would make in situ conversions of dithiocarbamates into the target ITCs quick, easy, and efficient.
In addition to the synthesis of isothiocyanates performed under conventional conditions, microwave-assisted synthesis of ITCs using primary amines and carbon disulfide as substrates were recently developed [217]. This approach enables synthesis of aliphatic and aromatic ITCs in high yields. Transformation of intermediate dithiocarbamates into ITCs occurs without the addition of any desulfurizing agent.

Synthesis ITCs via the Tandem Staudinger/aza-Wittig Reaction
The tandem Staudinger/aza-Wittig reaction is an alternative and equally efficient method used for the preparation of ITCs. The Staudinger reaction is the reaction of organic azide with a tricoordinated organophosphorus compound (triphenylphosphine (Ph 3 P), triphenyl phosphite, or triethyl phosphite) leading an iminophosphorane with the losses of nitrogen ( Figure 13a) [218]. The use of the aza-Wittig reaction for ITC synthesis was first reported by Molina et al. [219]. It involves the conversion of primary amines and dibromotriphenylphosphorane (triphenylphosphine dibromide) (PPh 3 PBr 2 ) into iminophosphoranes, followed by their reactions, with carbon disulfide (35) giving target ITCs (Figure 13b). The tandem Staudinger/aza-Wittig reaction was first described by Tsuge et al. [220] in 1984 ( Figure 13c). The advantage of the tandem reaction over the two-stage process, and the mechanistic aspects of this transformation were reported in 2006 by Isoda et al. [221]. In a tandem reaction, iminophosphorane formed from azide and Ph 3 P reacts in situ with CS 2 , "giving" the target ITCs in high yield ( Figure 13c). However, problems may occasionally occur with the separation of ITCs from an equimolar amount of triphenylphosphine sulfide formed in the reaction, and small amounts of triphenylphosphine oxide (a by-product of Ph 3 P oxidation).

The Latest Approaches to ITC Syntheses
In the past few years, several efficient approaches to the syntheses of ITCs were developed. They utilize primary amines as starting materials, and the appropriate fluorine reagents as desulfurizing agents, replacing toxic thiophosgene or carbon disulfide ( Figure 14).
Liao et al. [222] used primary amines as substrates, and the Langlois reagent (CF 3 SO 2 Na) [223] in the presence of copper iodide and diethyl phosphonate, to obtain a library of structurally diverse aromatic as well as aliphatic ITCs in high yields ( Figure 14, path a). The replacement of toxic thiophosgene with the Langlois reagent resulted in the development of a simple, safe, and environmentally friendly method of ITC synthesis. The disadvantages of this method include long reaction times, high temperatures, and low functional group tolerance (e.g., pyridinyl).
A highly efficient, selective, and fast method for the synthesis of ITCs from primary amines, by using a bench-stable, solid reagent (Me 4 N)SCF 3 (tetramethylammonium trifluoromethane thiosulfate), was described by Schoenebeck et al. [224] (Figure 14, path b). The target ITCs are easily separated from the solid side products, and the method is compatible with several functional groups. In addition, the use of (Me 4 N)SCF 3 enables the transformation of secondary diamines into cyclic thioureas. An alternative method of ITC preparation involves the reaction of thiocarbonyl fluoride (CF 2 =S), generated from difluorocarbene and elemental sulfur (S 8 ), with primary amines (Figure 14, path c) [225]. Difluorocarbene is valuable and versatile, intermediate in organic synthesis, and is generated from Ph 3 P + CF 2 CO 2 − (PDFA) [226]. Synthesis of ITCs is very fast, and the series of aromatic isothiocyanates with electron-donating and electronwithdrawing groups in the aromatic ring, as well as aliphatic ITCs, have been obtained in high yields. The only disadvantage of this method is that PDFA is relatively expensive, and in the reaction of o-phenylenediamines with the PDFA/S 8 system, difluoromethylthiolated heterocycles are formed.
The methodology using thiocarbonyl fluoride was also reported by Zhen et al. [227]. The authors obtained a pool of aromatic and aliphatic ITCs, in moderate to good yields, in the reaction of thiocarbonyl fluoride generated from CF 3 SiMe 3 (the Ruppert-Prakash reagent), elemental sulfur, and KF or AgSCF 3 at mild conditions ( Figure 14, path d). The Ruppert-Prakash reagent is a stable, relatively cheap, easy to handle and widely used reagent [228].
In 2019, Feng and Zhang [229] established an organophosphine-free, one-pot, coppercatalyzed, three-component synthesis of ITCs from primary amines, sodium bromodifluoroacetate (BrCF 2 CO 2 Na), and elemental sulfur (S 8 ) in the presence of K 3 PO 4 , as a base ( Figure 14, path e). According to the authors, isothiocyanation of amines takes place through the intermediate thiocarbonyl fluoride, or alternatively, via intermediate isocyanide, followed by its reaction with sulfur-this despite the fact that the reaction requires a prolonged time and a high temperature for completion, compatible with different functional groups, and a series of aromatic as well as aliphatic isothiocyanates have been obtained in moderate to good yields. For o-phenylenediamine and o-hydroxyaniline, as substrates, the appropriate 1-difluoromethyl benzimidazole and benzoxazole have been obtained, respectively. This and other methods of synthesis isothiocyanates using element sulfur have been described in a recently published review article [230].
Recently, Wei et al. [231] published an efficient method synthesis of a library of isothiocyanates from primary amines using trifluoromethanesulfonyl chloride in the presence of reducing the agent triphenylphosphine and sodium iodide ( Figure 14, path f ). Trifluoromethanesulfonyl chloride (CF 3 SO 2 Cl) is commercially available, cheap, easy to handle, and widely used (e.g., in electrophilic chlorination [232], trifluoromethylation [233], or in chloro-trifluoromethylthiolation of alkenes and alkyne [234]) reagents. The authors obtained aromatic ITCs with electron-donating or electron-withdrawing groups as well as aliphatic ITCs with good yields. Except for ITCs, the authors synthesized thiocarbamoyl fluorides using secondary amines with good yields.

Synthesis of Bifunctional Analogs of Sulforaphane and Their Properties
This section focuses on the most commonly used methods for the synthesis of SFN and its difunctional analogues. This involves isothiocyanation of amines with thiophosgene or carbon disulfide/desulfurizing agents system, or the tandem Staudinger/aza-Wittig method using azides as substrates. The methods where SFN and its analogues are obtained by myrosinase catalyzed hydrolysis of glucosinolates isolated from Cruciferae [235][236][237], or obtained by other methods (e.g., reaction with KSCN [238]), are not included. The choice is restricted to SFN and its natural or synthetic bifunctional analogues with an unbranched alkyl chain containing two to six carbon atoms and having sulfinyl, sulfanyl, sulfonyl, phosphonate, phosphinate, phosphine oxide, carbonyl, ester, amide, ether, or a second isothiocyanate group. Except for the synthesis, the biological activity of SFN and its analogues are also discussed.

Compound
Low IC 50 50 for NCI/ADR RES (human breast carcinoma), and erucin for HCI-H460 (human breast carcinoma). IC 50 on NmuMG for SFN and erysolin was almost similar; however, for erucin, it was unquestionably higher (Table 1).
A large group of ITCs can be considered as SFN analogues, having non-methyl substituents on the sulfinyl group. These compounds include fluorine derivatives of SFN, synthesized by Kiełbasiński and co-workers [244]. Thus, substrate 59 was converted into a trifluoromethyl derivative 70, and transformed the target product 73 via two independent routes. In the first one, amine hydrochloride 71, obtained after deprotection of amino group of compound 70, was converted into isothiocyanate 72 with a yield of 40% by the reaction with thiophosgene (34) or di-2-pyridyl thiocarbonate (38). Oxidation of the trifluoromethylsulfanyl group of 72, using MCPBA, gave the trifluoromethyl analog of SFN 73 a 50% yield. In the second synthetic pathway, phthalimido-sulfide 70 was oxidized to sulfoxide 74, followed by hydrazinolysis and subsequent isothiocyanation; thus, forming amine hydrochloride with thiophosgene (34) to give the target isothiocyanate 73 in a 87% yield. Using the first synthetic route, the authors also obtained trifluoroethyl analogues of SFN 75 and alyssin 76 with high yields (Figure 20). The ITCs 73, 75-76, obtained as racemates, were separated on preparative chiral HPLC to the enantiomerically pure products. All three pairs of enantiomers of fluorine-containing analogs 73, 75-76 were tested in vitro for their cytotoxicity against malignant melanoma cell lines Malme-3M and normal skin fibroblast Malme-3. In Table 2, the activity of the most promising compound 75 is presented.  25 35 After 48 h of incubation, the optically active fluorine analogs (R)-75 and (S)-75 exhibited higher cytotoxicity than SFN; however, after 72 h of incubation, the cytotoxicity was comparable. These results could suggest that the anticancer mechanism of SFN and its fluorine analogs are different. As seen from Table 2, the most promising was (S)-1isothiocyanato-4-((2,2,2-trifluoroethyl)sulfinyl)butane (S)-75).
Recently, the same research group described synthesis and biological activity of fluoroaryl analogs of SFN with 4 and 5 carbon atoms in the unbranched alkyl chain [245]. Thus, the starting ω-aminoalk-1-yl fluoroaryl or fluoroarylmethyl sulfides 77 were converted to the final fluoroaryl or fluoroarylmethyl analogs of SFN 80a-h by two alternative pathways ( Figure 21). In the first path, amines 77 were transformed in the reaction with thiophosgene (34) into fluoroaryl or fluoroarylmethyl ω-isothiocyanatoalk-1-yl sulfides 78, followed by oxidation with MCPBA, to afford the target isothiocyanates 80a-h. In the second approach, sulfides 77 were oxidized to sulfoxides 79, followed by the treatment with thiophosgene, to provide 80a-h. Final α-(fluorosulfinyl)and α-(fluoroarylmethylsulfinyl)ω-isothiocyanatoalkanes 80a-h were obtained in good and very good yields.  (Table 3). Table 3. The IC 50 (µM) for 80d and 80e and SFN after 72 h of incubation (mean ± SD).
It was found that fluoroaryl or fluoroarylmethyl analogs 80a-h, as well as SFN, were inactive against Gram-negative bacteria (E. coli and P. aeruginosa). However, SFN and its fluoroaryl or fluoroarylmethyl analogs 80b-f had antibacterial activity against Grampositive bacteria, including methicillin-resistant S. aureus (MARS), except for B. subtilis and E. hirae strains. In particular, ITC 80e was the most active (MIC values were in the range of 0.031-0.0625). Concerning anti-HIV activity, only ITC 80e showed similar activity to SFN (0.5 µM inhibited HIV replication in 9% of cases). Other ITCs were inactive.
In 2016, Shi et al. [246] synthesized a series of analogues of SFN, in which the methyl group adjacent to sulfur was replaced with heterocyclic moieties, such as furan (a), 5methoxy-3H-imidazo [4,5- thiazole (e). The sulfides 81a-e were transformed into amines 82a-e, and then were converted into sulfide derivatives of ITCs 83a-e with thiophosgene (34), in good and very good yields. In the next step, compounds 83d-e were oxidized with MCPBA into sulfoxide analogues of SFN 84d-e with good yields (Figure 22). Sulfoxide and sulfone analogues of SFN with heterocyclic moieties 84a-c and 89a-c were prepared in a modified way. Compounds 81a-c were oxidized by tert-butyl hydroperoxide (TBHP) to sulfoxides 85a-c and sulfones 86a-c and then transformed to amines 87a-c and 88a-c using methylamine. In the final step, isothiocyanation of amines 87a-c and 88a-c with thiophosgene afforded ITCs 84a-c and 89a-c in good yields ( Figure 23). Sulfone analogues with tetrazole and thiazole moieties 89d-e were obtained from amines 82d-e prior to Boc protection of amino groups, and oxidation formed N-Boc amines to sulfone analogues 90d-e. Removal of the Boc protecting group in 90d-e, followed by the reaction free amines 88d-e with thiophosgene, allowed to obtain the final sulfone analogues 89d-e in low yields ( Figure 24). The series of SFN analogues with heterocyclic moieties 83a-e, 84a-e, and 89a-e were evaluated for their anticancer activities (breast cancer cell lines MCF-7 and SUM-159, acute leukemia stem cell-like cell line, and KG-1a). Among all synthesized analogues, tetrazole analogues 83d, 84d, and 89d were generally the most potent-and significantly more active-than SFN against the tree cancer cell lines (Table 4). Moreover, compound 83d, as well as SFN, induced apoptosis in the SUM-159 cell line by increasing caspase-3 activity and significantly reducing the ALDH+ subpopulation in the SUM-159 cell line from 3.10% to 0.16%.
Recently Sestito et al. [247] designed and synthesized a new class of multitarget H2S-donor hybrids 99-104, combining the rivastigmine-scaffold, an acetylcholinesterase inhibitor with brain region selectivity [248], a well-known drug approved for Alzheimer's disease, with SFN and erucin. Thus, mercaptobutyl derivative 91 was alkylated with the appropriate chloroacetamide 92a-c under the basic conditions to give the corresponding thioethers 93-95 with moderate yields. Hydrazinolysis of derivatives 93-95 provided amines 96-98, which, after the reaction with thiophosgene, gave ITCs 99-101 in 25-90% yields. Oxidation of ITCs 99-101 by Oxone ® led to sulfoxide 102-104 in moderate yield ( Figure 25). Studies on the murine microglia cell line (BV-2) showed that all synthesized ITC hybrids 99-104 exhibited a H 2 S-donor profile in vitro. Compounds 99-104 showed significantly anti-inflammatory and antioxidant activities and induced the expression of proteins (i.e., GSH) involved in the antioxidant defense in the neuronal cell line. All hybrids produced a significant decrease in ROS production elicited by pro-inflammatory stimulus compared to the rivastigmine, which completely lacks antioxidant activity. The new hybrids were also able to reduce NO release in BV-2 cells, whereas rivastigmine showed no effect. Moreover, the most active compounds 99 and 100 increased the GSH level in the human neuroblastoma cell line SH-SY5Y.
In 2013, Hu et al. [249] obtained an extensive library of sulfanyl and sulfinyl analogs of SFN with an alkyl or phenyl substituent on a sulfur atom, and with an alkyl linker containing 3 to 6 carbon atoms using the dithiocarbamate approach and applying mesyl chloride (48)   All synthesized ITCs 28, 24, 148-167 were evaluated in vitro for their cytotoxicity against liver hepatocellular carcinoma (HepG2), human lung adenocarcinoma (A549), woman breast cancer ER+ (Luminal A)(MCF-7), human colon cancer cell line (HCT-116) and human neuroblastoma cell line (SH-SY5Y). All tested compounds exhibited more potent inhibitory against five cancer cell lines than SFN. The IC 50 for the most active ITCs are presented in Table 5.  28, 24, 148-167, it was found that compounds with sulfanyl moiety 135 showed weaker inhibitory effects than most derivatives; however, compound 168 with a sulfone group showed a higher inhibitory activity against all cancer cell lines than SFN. It may indicate that replacing the sulfoxide group with a sulfone group results in higher biological activity. The studies on anticancer mechanisms examined on the HepG2 cancer cell line showed that SFN, as well as model ITC 155, could induce the S or G 2 /M phase cycle arrest, and 155 has more potent inhibitory activity than SFN. Moreover, ITC 155 exhibited greater induction of apoptosis upon treatment than SFN. ITC 155 presented a time-and dose-dependent activation on the Nrf2 transcription factor. Moreover, 155 acted as a more potent Nrf2 inducer than SFN.
Meijer and co-workers [163] synthesized SFN (24) and its analogue erucin (28) via the tandem Staudinger/aza-Wittig reaction. Thus, the starting 4-bromo-butanol (169) was transformed using a standard procedure into azido thioether 170. Then, this compound, in the reaction with triphenylphosphine and carbon disulfide, was converted into erucin (28) in an 81% yield. SFN (24) was obtained by oxidation of erucin (28) with MCPBA in 77% yield ( Figure 27). Studies on P. aeruginosa show that SFN and erucin strongly inhibit quorum sensing (QS) and virulence (biofilm formation and pyocyanin production). The assays on P. aeruginosa and in E. coli strongly indicate that SFN and erucin effectively bind LasR, resulting in inhibition of QS activation at concentrations that can be found in broccoli.
The Staudinger/aza-Wittig tandem reaction was used in the synthesis of enantiomerically pure (R)-SFN ((R)-24) [250] using diacetone-D-glucofuranose (171) (DAG)methodology [251]. The stereoselective synthesis of SFN was based on the reaction of 1-azidobutanesulfinyl chloride (172) with DAG (171), using Hünig's base as a catalyst, and affording the sulfinate ester (S)-173 in a 90% yield and in 94% diastereomeric excess. Reaction of methylmagnesium bromide with the sulfinate ester (S)-173 provided 4-azidobutyl methyl sulfoxide ((R)-174) with inversion of configuration, which, in turn, under the Staudinger/aza-Wittig reaction with triphenylphosphine and carbon disulfide, gave enantiopure (R)-SFN ((R)-24) in a 71% yield. The same methodology was applied in the synthesis of the other analogues. Thus, condensation of the selected Grignard reagents with sulfinate ester (S)-173 led to the desired azido sulfoxides, which, in a two-step Staudinger/aza-Wittig reaction, allowed obtaining enantiopure (R)-sulfinyl ITCs 175-178 in 47-90% yields ( Figure 28).    In the same way, using ethylmagnesium bromide or butylmagnesium bromide as a Grignard reagent, the authors synthesized analogues of SFN, in which the methyl group was replaced by ethyl or butyl substituents. Enantiopure sulfinyl analogues (R)-187 and (R)-188 were prepared with high yields (Figure 32). The efficiency of the synthesized compounds as the inductors of phase II detoxifying enzymes was evaluated by studying their ability to activate the cytoprotective transcription factor Nrf2. It was shown that homologues containing 5 and 6 carbon atoms in the alkyl chain showed beneficial effects on the activation of Nrf2, increasing the steric size of the substituent on the sulfur has negative effects on the biological activity. Which sulfur stereochemistry has no effect on the ability of these analogues to activate the cytoprotective transcription factor Nrf2 is additionally noted ( Table 6). All synthesized (R) and (S) analogues of SFN were evaluated in vitro for their cytotoxicity against human lung adenocarcinoma (A549) and the fetal lung fibroblast (MRC-5) cell line as normal cells. Synthesized analogues were more effective against lung cancer than (R)-SFN, and among them, (S)-184 was the most promising compound that showed slight selectivity than SFN towards the cancer cell (Table 6).

Synthesis of Phosphorus Analogues of Sulforaphane and Their Properties
In this section, phosphorus analogues of sulforaphane (P-SFN) compounds, where methylsulfinyl moiety was replaced by phosphonate, phosphinate, or phosphine oxide groups, are described.
In 2011, Oleksyszyn et al. [200] described the synthesis of a series of αand βdialkoxyphosphoryl isothiocyanates. Dialkyl α-(isothiocyanatoalkyl)phosphonates 204-215 were obtained in two ways, as shown in Figure 34. In the first way (method A) for the transformation of dialkyl α-azidoalkylphosphonates 192-198 into isothiocyanates 204-210, the tandem Staudinger/aza-Wittig reaction was used, and the final P-SFN were obtained in 35-75% yields. In the second way (method B), the targets P-SFN 211-215 were prepared with good and very good yields via desulfuration of dithiocarbamates formed in situ from aminophosphonates hydrochlorides 199-203 and carbon disulfide with TBTU. Method B was also used for the synthesis of β-dialkoxyphosphoryl isothiocyanates 216-218 ( Figure 34).
The obtained P-SFN were tested for cytotoxicity on five cancer lines: lung cancer (A549), breast cancer (T47D and MCF-7), colon cancer (LoVo), as well as its doxorubicinresistant variant-LoVo/DX. All tested αand β-dialkoxyphosphoryl isothiocyanates showed very good antiproliferative activities in vitro comparable to the most active of natural isothiocyanates BITC (21) and PEITC (22). Moreover, the mechanism of anticancer activity was evaluated. Isothiocyanate 205 showed inhibition of the cell cycle in the subG 0 /G 1 phase, and compound 216 inhibited the cell cycle in the G 2 /M phase.  The in vitro antiproliferative activities of synthesized ITCs 237-246 against LoVo, LoVo/DX, A549, and MCF-7 cell lines were in the range of natural isothiocyanates despite the significant differences in their structures. Among them, ITC 246 was the most active (Table 8). In 2017, Gajda and Wietrzyk and co-workers [254] designed and synthesized a library of novel bifunctional SFN analogues 282-316, structurally diverse dialkyl, and diphenyl ω-(isothiocyanato)alkylphosphonates (P-ITCs) with an unbranched alkyl side chain containing 2 to 6 carbon atoms. The synthesis of P-ITCs was based on the conversion of dialkyl [255] as well as diphenyl ω-azidoalkylphosphonates 247-281 into the target dialkyl and diphenyl ω-(isothiocyanato)alkylphosphonates 282-316, using the tandem Staudinger/aza-Wittig reaction with triphenylphosphine and carbon disulfide with good and very good yields ( Figure 37). The authors developed a one-pot strategy enabling the direct conversion of ω-(isothi ocyanato)alkylphosphonates 287-291 into the selected alkyl, or phenyl ω-(isothiocyanato) alkylphosphonates 282-286, 297, 302, 316-325 in moderate and very high yields ( Figure 38). All synthesized P-ITCs 282-325 were evaluated in vitro for antiproliferative activity against the colorectal adenocarcinoma cell line LoVo and its doxorubicin-resistant subline LoVo/DX. SFN, and other natural isothiocyanates, such as BITC or AITC, were used as references. All tested compounds 282-325 showed high activity on LoVo and LoVo/DX, higher than natural ITCs (SFN and BITC). The most active were ω-(isothiocyanato)alkylphosphonates with branched isopropyl 293-297 and isobutyl 298-302 groups on phosphorus. The activity was, for some compounds, more than 10 times higher than SFN activity (Table 9). In addition, the antiproliferative activity of selected P-ITCs 291, 297, and 316 were tested on seven cancer cell lines: murine mammary gland cancer (4T1), leukemia (HL60) and its subline resistant for mitoxantrone (HL60/MX2), non-small lung cancer (A549), uterus sarcoma (MESSA and MESSA/Dx-5), and normal murine fibroblast (BALB/3T3). Compounds 291 (IC 50 = 0.5 ± 0.2 µM) and 316 (IC 50 = 0.8 ± 0.2 µM) showed similar activity to cisplatin (CDDP) (IC 50 = 0.4 ± 0.2 µM), relative to HL60 cells. Research with the selected P-ITCs 291, 297, and 316, demonstrated that P-ITCs inhibited the G 2 /M cell cycle on LoVo and LoVo/DX, where P-ITC 316 was more active than SFN, as well as P-ITCs 291, 297, and 316-induced apoptosis. In vivo studies of the selected P-ITCs showed slightly lower anticancer and antimetastatic activity in comparison to naturally occurring BITC. However, recent studies in vivo on Zebrafish have shown that P-SFN 291 was characterized by high anticancer activity and low toxicity [256].
Diaryl ω-(isothiocyanato)alkylphosphonates 378-411 were evaluated in vitro for antibacterial activity on S. aureus and P. aeruginosa strains in comparison with natural PEITC and with gentamicin used as a reference antibiotic. All synthesized P-ITCs characterized high antibacterial activity, higher than natural PEITC. Against the S. aureus strain, the most active were 379 (IC 50 = 1.5 ± 0.1 µM) and 383 (IC 50 = 2.5 ± 0.2 µM) with activity similar to the gentamicin (IC 50 = 1.0 ± 0.1 µM). Against the P. aeruginosa strain, the most active were 381, 383-384, 388-389, with IC 50 between 3.0 ± 2.3 and 4.0 ± 1.6 µM. The selected compounds were three-and four-times more active than gentamicin (IC 50 = 12 ± 1.0 µM). Except for antibacterial activity, all P-ITCs were evaluated for antiproliferative activity against LoVo and LoVo/DX cancer call lines. All compounds presented high antiproliferative activity, higher or similar to SFN. The most active was compound 407 (IC 50 = 1.0 ± 0.1 µM), which was 3 times higher than cytostatic CDDP, and more than 22 times higher than SFN.
Selected diaryl ω-(isothiocyanato)alkylphosphonates 379, 383, 388, and 393 were also converted into mercapturic acid 412-415 derivatives in the reaction with N-acetyl-L-cysteine (NAC) and sodium bicarbonate, followed by acidification in good yields ( Figure 44). Preliminary evaluation of the in vitro antibacterial activity on the S. aureus strain showed that mercapturic acid 412-415 derivatives exhibited moderate antibacterial activity, lower or similar to the parent isothiocyanates 379, 383, 388, and 393. More accurate research concerning synthesis and antiproliferative activity of phosphonates, phosphinates, and phosphine oxide isothiocyanate-derived mercapturic acids, were described by Psurski and co-workers [259].

Synthesis of Carbonyl and Amide Analogues of Sulforaphane and Their Properties
The next group of SFN analogues are molecules containing carbonyl, ester, and amide moieties. Posner et al. [188] used the same methodology with thiophosgene, shown in Figure 33 for synthesis of carbonyl analogue of SFN, where methylsulfinyl moiety was replaced by carbonyl group (419). In this way, δ-valerolactam (416), in the reaction with di-tert-butyl dicarbonate and methylmagnesium iodide. was transformed into 4-N-Bocaminobutyl methyl ketone (417). Ketone 417 under hydrolytic conditions was converted to the 4-aminobutyl-methyl ketone (418), followed by the reaction with thiophosgene to afford the 2-oxohexyl isothiocyanate (419) with an overall yield of 6% from lactam 413 ( Figure 45). Carbonyl analogue 419 of SFN, as well as SFN (24), were evaluated in vitro as inducer potencies of NAD(P)H:quinine oxidoreductase (QR) in murine hepatoma cells (Hepa 1c1c7) ( Table 10). As seen in Table 10, the 2-oxohexyl isothiocyanate (419) and SFN (24) were the same in potency as the inducer of NAD(P)H:quinine oxidoreductase.
In 2009, Amara et al. [260] synthesized carbonyl analogues of SFN with a longer alkyl chain containing 8 to 10 carbon atoms, being inhibitors of quorum sensing in P. aeruginosa. As a template, the authors used a structure of a natural autoinducer of P. aeruginosa-3-oxo-C12-N-acyl homoserine lactone (3-oxo-C12-HSL, 420). Final ITCs 424-427 were obtained with good and very good yields from the appropriate azides 421-423 in the tandem Staudinger/aza-Wittig reaction with triphenylphosphine and carbon disulfide ( Figure 46). The inhibitions of quorum sensing of P. aeruginosa by ITCs 424-426 was evaluated using the luminescent PAO1-luxABCDE wild type strain. All synthesized ITCs 424-426 strongly inhibited luminescence in this wild type strain. The strongest inhibitor of luminescence appeared to be 426 (IC 50 = 45.2 ± 0.7 µM), followed by 425 (IC 50 = 113 ± 19 µM) and 424 (~300 µM). Additionally, in the assay with the wild type PAO1 strain, ITC 425 presented significant inhibition of the quorum sensing-controlled virulence factor expression, as well as biofilm formation. The authors showed that the obtained isothiocyanate-based probes covalently and selectively bound Cys79, found in the LasR-binding pocket.
The same research group synthesized the second generation of ITCs based on ITC 425 with halogen moieties (fluorine, bromine, and chlorine) at the β-position [261]. As in previous studies, azides 427-429 were transformed into ITCs 430-432 in the reaction with triphenylphosphine and carbon disulfide with good yields (Figure 47). The bioactivity of new ITCs 430-432 were evaluated using P. aeruginosa PAO1-UW and PAO-JP2 (lasl/rhll double mutant) strains carrying the luxCDABE cassette as reporters for LasR activation. In this tests, ITC 430 showed more complete inhibition of LasR than parental ITC 425. On the other hand, ITCs 431 and 432 had higher inhibition of LasR than fluorine-analogue of ITC. Additionally, ITC 430 inhibited pyocyanin production by almost 40%, and its inhibition was stronger than in the presence of ITC 425. Moreover, ITC 430 caused a swarming inhibition of 44% on agar plates, a stronger effect than that observed for ITC 425. Other ITCs, 431 and 432, did not inhibit swarming at all. The authors also showed that, in vivo, ITC 430 increased survival of C. elegans from bacterial infections over the course of four days.
Compounds 437-439 and 443 had almost similar antiproliferative and anti-NO production activities at those of ITC 433. ITCs 440 and 444 were less active than ITC 443. Thiocyanate 445, compared with ITC 433, had no activity, which shows that the isothiocyanate group is responsible for biological activity.
Milelli, Minarini, and co-workers [263] described synthesis of novel quinazoline derivatives with polymethylene-amide linkers of different lengths, and possessing an isothiocyanate group, 451-455, or derivatives, such as 457, where NCS moiety was directly connected to the aromatic ring. In substrates 446-450 and 456, isothiocyanation of amino groups was accomplished with 1,1 -thiocarbonyldi-2,2 -pyridone (37) in satisfactory yields ( Figure 50).  Synthesis of 1-isothiocyanato-6-methoxyhexane (443) and 1-isothiocyanato-6-(methylthio)hexane (444). ITCs 451-455 and 457 were evaluated for their ability to inhibit the proliferation of the highly epidermal growth factor receptor (EGFR-TK) expressed in human epithelial cancer cells A431 and HaCaT cancer cells. ITCs 451-455 had similar activity on the A431 cell line (IC 50 between 14.38 ± 1.5 µM and 17.06 ± 1.87 µM) to SFN (IC 50 = 15.76 ± 1.89 µM), and also similar activity on the HaCaT cell line. The most active was ITC 457, which IC 50 on the A431 cell line was much higher than SFN and other ITCs (IC 50 = 2.04 ± 0.22 µM) and also higher on HaCaT (IC 50 = 2.62 ± 0.31 µM). Which ITC 457 did not affect the normal cell proliferation of human fibroblasts (HGFs) is noteworthy. The inhibitory effect of new ITCs 451-455 and 457 on EGFR-TK activity evaluated on A431 cell lysates showed that ITC 457 had inhibition effects at the nanomolar concentrations with a maximum inhibition of 89% at 10 µM. ITCs 451-455 were weaker inhibitors. Additionally, treatment of A431 cells with ITC 457 at 2 µM led to apoptosis, in terms of DNA fragmentation. In 2019, Boehm and co-workers [264] synthesized crystalline SFN analogues 459 and 461. Both isothiocyanates 459 and 461 were obtained in good yields, in a two-step reaction of the appropriate amine hydrochlorides 458 or 460, and carbon disulfide in the presence of triethylamine, using hydrogen peroxide or tosyl chloride as desulfurizing agents ( Figure 51). The dialkyl tertiary squaramide 461 isolated as a crystalline solid with good solid-state stability was shown to be a covalent Nrf2 activator that binds to the BTB domain of KEAP1. Additionally, ITC 461 has demonstrated efficiency for activation of the Nrf2 pathway in human bronchial epithelial (BEAS2B) cells, translating to a dose-dependent inhibition of lung inflammation in an in vivo model of pulmonary oxidative stress.
Recently, Wang and co-workers [265] designed and synthesized a series of cyclindependent kinase 9 (CDK9) inhibitors with cancer stem cell (CSC) inhibition activity for non-small-cell lung cancer (NSCLC) therapy. The structures of the inhibitors were based on the combination of the pyrrolo-[2,3-d]pyrimidines-2-amine from ribociclib as CDK9 pharmacophore, and analogues of SFN with alkyl chains of different lengths as targeting CSC pharmacophore. Briefly, the key N-Boc amines 462a-e and 463aa-de were converted into the final ITCs 464a-e and 465aa-de after prior deprotection of the amino group by trifluoroacetic acid, followed by the reaction with carbon disulfide and N,N'dicyclohexylcarbodiimide (DCC, 45) (Figure 52). The same methodology was used to  transform amines 466, 467a-g, and 468 into ITCs 469, 470a-g, and 471 (Figure 53).   464a-e, 465aa-de, 469, 470a-g, and 471 were evaluated regarding inhibition activity against CDK4, CDK6, and CDK9, with ribociclib as the positive control. Some compounds showed high potent activity against CDK9 as well as high selectivity for CDK9. The inhibition of tested ITCs was similar or higher than inhibition of ribociclib. In particular, ITC 470e with an 8-isothiocyanatooctanamide linker, exhibited high enzymatic inhibition (IC 50 = 11 nM vs. IC 50 of ribociclib = 197 nM). ITC 470e was tested against several tumor cell lines, including NSCLC, breast cancer, hepatocarcinoma, cervical cancer, leukemia, and lymphoma, using CCK8 assay and SFN as positive controls. ITC 470e had the highest activity against NSCLC cell lines, especial A549 and H1299, with IC 50 values less than 0.5 µM. For comparison, IC 50 of SFN on A549 was more than 10 µM and IC 50 of ribociclib was 7.455 µM. For other cell lines, ITC 470e exhibited lower anti-viability activity. These results demonstrated good cellular selectivity of ITC 470e. Additionally, studies on A549 and H1299 showed that ITC 470e dose-dependently inhibited the cell cycle at G 2 /M phase and induced apoptosis of A549 cells in a concentration-dependent manner. Moreover, ITC 470e decreased the formation of colonies in the two NSCLC cell lines. In vivo studies on H1299 xenograft mouse models showed that ITC 470e displayed potent anti-tumor activities.

Synthesis of Ether-Linked Analogues of Sulforaphane and Their Properties
Perez and co-workers [266] synthesized ether-linked isothiocyanate as the LasR antagonist of quorum sensing in P. aeruginosa. Briefly, etherification of phenol 472, followed by amino group deprotection with TFA and a two-step reaction with carbon disulfide and molecular iodine, afforded ether-linked isothiocyanates 473 and 474 in low yields ( Figure 54). A LasR antagonist bioassay exhibited low IC 50 values (ITC 473 IC 50 > 200 µM and ITC 474 IC 50 = 145 ± 105 µM) with no effect on bacterial growth. In 2019, Bussolo and Minutolo et al. [267] synthesized novel glycoconjugated H 2 S donors with the isothiocyanate group. For this purpose, the starting N-Boc-glycoconjugates 475a-b were deprotected with TFA, followed by the reaction of amino derivatives 476a-b with an excess of carbon disulfide, triethylamine, and hydrogen peroxide to afford final, fully acetylated ITCs 478a-b, with low yields. On the other hand, deacetylation of Oacetylated amines 476a-b with sodium methanolate, followed by the isothiocyanation of amines 477a-b, performed in the same manner as above, gave isothiocyanate glycopyranosides 479a-b in 21% and 22% yields, respectively ( Figure 55).
ITCs 478b and 479a were evaluated for their cell inhibitory effects on viability, using pancreatic adenocarcinoma tumor cells (AsPC-1). Studies showed that glucose-derivative 479a was totally ineffective at inhibiting cell viability. However acetyl-galactosaminederivative 478b exhibited a marked cytotoxicity against AsPC-1 tumor cells in a concentrationdependent manner with a pIC 50 value of 4.45 ± 0.02. ITC 478b efficiently released H 2 S intracellularly with desirable slow kinetics and inhibition of cell cycle progression G 0 /G 1 phase. He and co-workers [268] designed and synthesized artemisinin-isothiocyanate derivatives with anti-glioblastoma effects. The artemisinin-isothiocyanate derivatives 481a-c were obtained in good yields from amines 480a-c in a two-step reaction with carbon disulfide and triethylamine used as a base, followed by desulfurization with acyl chloride (Figure 56). All ITCs 481a-c showed higher anti-tumor effects in vitro than dihydroartemisinin (DHA) against human glioblastoma U87 cells. The most active was ITC 481b (IC 50 = 7.41 ± 1.56 µM) (IC 50 DHA = 118.95 ± 12.39 µM). ITC 481b reduced the viability of glioblastoma multiforme (GBM) in a concentration-dependent manner. ITC 481b also inhibited migration and induced apoptosis in U87 cells. Pyknosis and nuclear shrinkages were observed in ITC 481b-treated cells. Additionally, caspase 9 and cytochrome c were induced in U87 cells treated with ITC 481b and cleaved caspase 3 was increased. On the other hand, the anti-apoptotic protein Bcl-2 was downregulated, and pro-apoptotic protein BAX was upregulated.

Synthesis of Diisothiocyanates
Oleksyszyn and co-workers [269] synthesized a library of diisothiocyanates (diITCs) as well as their mercapturic acid derivatives conjugated with N-acetyl cysteine. Both isothiocyanate functional groups in diITCs were separated with unbranched alkyl linkers containing three and four carbon atoms (484 and 485). DiITCs 484 and 4825 were obtained from the corresponding diamines 482 and 483 in a two-step procedure with carbon disulfide and triethylamine as a base, followed by the reaction with HBTU as desulfurizing reagents ( Figure 57). Synthesized diITC were evaluated in vitro on LoVo and LoVo/DX cancer cell lines. Their IC 50 values on the LoVo cancer cell line were very high and were lower than 2 µM. Additionally, their biological activities were higher than their mercapturic acid derivatives.

Summary of the Synthetic Routs of SFN and Its Bifunctional Analogs and Their Biological Activity
The described above methods of the synthesis of SFN and its bifunctional analogs are presented in Table 12, divided into various functional groups.
Based on the Table 12, it can be seen that the synthesis of SFN, and its sulfur analogs substituted with the methyl group, but also by varied aliphatic, aromatic, fluorine, or heterocyclic substituents, are the most described in the literature. Among them, the method using thiophosgene (CSCl 2 ) is dominant. The next often used method is the tandem Staudinger/aza-Wittig reaction, and the least frequently used is the two-step method using a desulfurizing agent. Another group involves phosphorus analogues of SFN (P-SFN) with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl 2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of ether-linked analogs of SFN and diisothiocyanates, was not used. Table 12. Summary of the synthetic routes of SFN and its bifunctional analogs.

Reaction Reference
Synthesis of SFN and its sulfur analogues genic bacteria, fungi, and molds were evaluated. DiITCs 485, 490-493, were more active against Gram-positive bacteria (B. cereus; MIC = 2 μg/mL) than Gram-negative (E. coli; MIC > 64 μg/mL). Studies on wide selections of Gram-negative as well as Gram-positive strains showed that the most active were diITCs 492 and 493, which, on Gram-positive strains, such as Streptococcus, Staphylococcus, and Bacillus, exhibited high activity (MIC= 1-2 μg/mL). DiITCs 492 and 493 presented high activity against mycobacteria. The most active were against M. bovis and M. tuberculosis (MIC = 2-4 μg/mL) than against nontuberculous mycobacteria. DiITC 492 efficiently inhibited the growth of the pathogens, such as Candida albicans or Candida glabrata, for which MIC < 1 μg/mL.

Summary of the Synthetic Routs of SFN and Its Bifunctional Analogs and Their Biological Activity
The described above methods of the synthesis of SFN and its bifunctional analogs are presented in Table 12, divided into various functional groups. Table 12. Summary of the synthetic routes of SFN and its bifunctional analogs.

Summary of the Synthetic Routs of SFN and Its Bifunctional Analogs and Their Biological Activity
The described above methods of the synthesis of SFN and its bifunctional analogs are presented in Table 12, divided into various functional groups. Table 12. Summary of the synthetic routes of SFN and its bifunctional analogs.

Summary of the Synthetic Routs of SFN and Its Bifunctional Analogs and Their Biological Activity
The described above methods of the synthesis of SFN and its bifunctional analogs are presented in Table 12, divided into various functional groups. Table 12. Summary of the synthetic routes of SFN and its bifunctional analogs.

Summary of the Synthetic Routs of SFN and Its Bifunctional Analogs and Their Biological Activity
The described above methods of the synthesis of SFN and its bifunctional analogs are presented in Table 12, divided into various functional groups. Table 12. Summary of the synthetic routes of SFN and its bifunctional analogs.

Synthesis of diisothiocyanates
[269] [55] Based on the Table 12, it can be seen that the synthesis of SFN, and its sulfur analogs substituted with the methyl group, but also by varied aliphatic, aromatic, fluorine, or heterocyclic substituents, are the most described in the literature. Among them, the method using thiophosgene (CSCl2) is dominant. The next often used method is the tandem Staudinger/aza-Wittig reaction, and the least frequently used is the two-step method using a desulfurizing agent. Another group involves phosphorus analogues of SFN (P-SFN) [266][267][268] Synthesis of diisothiocyanates Synthesis of carbonyl and amide analogues of SFN [188,263] [ 264,265] [ [260][261][262] Synthesis of ether-linked analogues of SFN [266][267][268]

Synthesis of diisothiocyanates
[269] [55] Based on the Table 12, it can be seen that the synthesis of SFN, and its sulfur analogs substituted with the methyl group, but also by varied aliphatic, aromatic, fluorine, or heterocyclic substituents, are the most described in the literature. Among them, the method using thiophosgene (CSCl2) is dominant. The next often used method is the tandem Staudinger/aza-Wittig reaction, and the least frequently used is the two-step method using a desulfurizing agent. Another group involves phosphorus analogues of SFN (P-SFN)

Synthesis of diisothiocyanates
[269] [55] Based on the Table 12, it can be seen that the synthesis of SFN, and its sulfur analogs substituted with the methyl group, but also by varied aliphatic, aromatic, fluorine, or heterocyclic substituents, are the most described in the literature. Among them, the method using thiophosgene (CSCl2) is dominant. The next often used method is the tandem Staudinger/aza-Wittig reaction, and the least frequently used is the two-step method using a desulfurizing agent. Another group involves phosphorus analogues of SFN (P-SFN) [55] Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN. with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of etherlinked analogs of SFN and diisothiocyanates, was not used. Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN. with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of etherlinked analogs of SFN and diisothiocyanates, was not used. Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN. with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of etherlinked analogs of SFN and diisothiocyanates, was not used. Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN. with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of etherlinked analogs of SFN and diisothiocyanates, was not used. Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN. with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of etherlinked analogs of SFN and diisothiocyanates, was not used. Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN. with phosphonate, phosphinate, or phosphine oxide moiety with aliphatic or aromatic substituents. For this class of compounds, both the tandem Staudinger/aza-Wittig reaction and the method using a desulfurizing agent were equally frequently used. The reaction with CSCl2 for synthesis P-SFN was used less frequently. Carbonyl and amide analogs of SFN were synthesized by all three methods. The synthesis of ether-linked analogs of SFN was completely performed by the two-step methodology with a desulfurizing agent, while the synthesis of diisothiocyanates was also performed with the desulfurizing agent and thiophosgene. The tandem Staudinger/aza-Wittig reaction, to the synthesis of etherlinked analogs of SFN and diisothiocyanates, was not used. Table 13, summarizes the relationship between the biological activity of analogs of SFN and various functional groups. The table presents the most anticancer-active analogs of SFN containing sulfinyl, phosphonate, phosphinate, phosphine oxide, carbonyl, carboxamide, or additional isothiocyanate functional groups. Due to the fact that there are no differences in biological activity between SFN and its natural analogs, such as erucin or alyssin (see Figure 5), these compounds are not placed in Table 13. Only the analogs of SFN are placed in Table 13 for which biological activity is compared with SFN.  CD↔ [188] LoVo↑ LoVo/DX↑ [254] LoVo↑ LoVo/DX↑ [257] LoVo↑ LoVo/DX↑ [258] CD↔ [188] A431↔ [263] LoVo↑ LoVo/DX↑ [257] MCF-7↑ SUM-159↑ KG-1a↑ [246] HepG2↑ A549↑ MCF-7↑ HCT-116↑ SH-SY5Y↑ [249] CD↔ [188] LoVo↑ LoVo/DX↑ [254] LoVo↑ LoVo/DX↑ [257] LoVo↑ LoVo/DX↑ [258] CD↔ [188] A431↔ [263] LoVo↑ LoVo/DX↑ [258] MCF-7↑ SUM-159↑ KG-1a↑ [246] HepG2↑ A549↑ MCF-7↑ HCT-116↑ SH-SY5Y↑ [249] CD↔ [188] LoVo↑ LoVo/DX↑ [254] LoVo↑ LoVo/DX↑ [257] LoVo↑ LoVo/DX↑ [258] CD↔ [188] A431↔ [263] CD↔ [188] MCF-7↑ SUM-159↑ KG-1a↑ [246] HepG2↑ A549↑ MCF-7↑ HCT-116↑ SH-SY5Y↑ [249] CD↔ [188] LoVo↑ LoVo/DX↑ [254] LoVo↑ LoVo/DX↑ [257] LoVo↑ LoVo/DX↑ [258] CD↔ [188] A431↔ [263] A431↔ [263] Molecules 2022, 26 ↑-more active than SFN; ↓-less active than SFN; ↔-similar active to SFN.
Based on Table 13, it can be seen that replacing the methyl group in sulfinyl moiety in SFN with structurally diverse fluorine ((S)-75, 80d), heterocyclic (84d), or aliphatic (e.g., iso-butyl) (152) substituents result in increases of the biological activities of analogs of SFN on different cancer cell lines, such as Malme-3M, MCF-7, or A549. For phosphorus analogs of SFN (P-SFN), the presence of phosphine oxide moiety with methyl substituents (191) does not affect biological activity. However, replacement of the methyl sulfinyl group by phosphonate moiety with aliphatic (298) or aromatic (407) substituents or phosphinate moiety (372) results in an increase in the anticancer activity on LoVo and LoVo/DX cancer cell lines. Carbonyl or amide analogs of SFN (419 and 455) can by characterized by biological activities similar to SFN, but on the other hand, for some analogs (e.g., 470e), biological activity could be higher than for SFN. The presence of the second isothiocyanato group (484) also results in an increase in the biological activity on LoVo and LoVo/DX cancer cell lines.

Conclusions
There are many examples describing the synthesis of SFN and its natural or synthetic difunctional analogues, with methyl sulfinyl group replaced by sulfinyl, sulfanyl, sulfonyl, phosphonate, phosphinate, phosphine oxide, carbonyl, ester, amide, ether, or a second isothiocyanate group. The most common strategies involve the use of thiophosgene as a thiocarbonyl transfer reagent, utilization of a carbon disulfide/desulfurizing agent system (dithiocarbamates approach), or use of the tandem Staudinger/aza-Wittig reaction (triphenylphosphine/carbon disulfide system). In the first two approaches, primary amines are utilized as substrates; the last comprises azides as starting materials. The choice of method depends on the availability of starting substrates and sensitivity of functional groups present in the substrates to the reaction conditions. Several ITCs are characterized by high biological activity (anticancer or antibacterial), usually higher than parental SFN. Which modifications of the structure of SFN seem to have important impacts on the biological activities of the new analogues of SFN.
As SFN is biologically active, natural ITC is found in cruciferous vegetables, and it is non-toxic-it has been selected for phase I and II clinical trials, where it is administered in the form of an extract or broccoli sprouts. The results of these studies are promising and indicate which SFN, in the future, may be considered as an anticancer drug. The situation is different for synthetic analogs of SFN, which have been described in this review, having ↑-more active than SFN; ↓-less active than SFN; ↔-similar active to SFN.
Based on Table 13, it can be seen that replacing the methyl group in sulfinyl moiety in SFN with structurally diverse fluorine ((S)-75, 80d), heterocyclic (84d), or aliphatic (e.g., iso-butyl) (152) substituents result in increases of the biological activities of analogs of SFN on different cancer cell lines, such as Malme-3M, MCF-7, or A549. For phosphorus analogs of SFN (P-SFN), the presence of phosphine oxide moiety with methyl substituents (191) does not affect biological activity. However, replacement of the methyl sulfinyl group by phosphonate moiety with aliphatic (298) or aromatic (407) substituents or phosphinate moiety (372) results in an increase in the anticancer activity on LoVo and LoVo/DX cancer cell lines. Carbonyl or amide analogs of SFN (419 and 455) can by characterized by biological activities similar to SFN, but on the other hand, for some analogs (e.g., 470e), biological activity could be higher than for SFN. The presence of the second isothiocyanato group (484) also results in an increase in the biological activity on LoVo and LoVo/DX cancer cell lines.

Conclusions
There are many examples describing the synthesis of SFN and its natural or synthetic difunctional analogues, with methyl sulfinyl group replaced by sulfinyl, sulfanyl, sulfonyl, phosphonate, phosphinate, phosphine oxide, carbonyl, ester, amide, ether, or a second isothiocyanate group. The most common strategies involve the use of thiophosgene as a thiocarbonyl transfer reagent, utilization of a carbon disulfide/desulfurizing agent system (dithiocarbamates approach), or use of the tandem Staudinger/aza-Wittig reaction (triphenylphosphine/carbon disulfide system). In the first two approaches, primary amines are utilized as substrates; the last comprises azides as starting materials. The choice of method depends on the availability of starting substrates and sensitivity of functional groups present in the substrates to the reaction conditions. Several ITCs are characterized by high biological activity (anticancer or antibacterial), usually higher than parental SFN. Which modifications of the structure of SFN seem to have important impacts on the biological activities of the new analogues of SFN.
As SFN is biologically active, natural ITC is found in cruciferous vegetables, and it is non-toxic-it has been selected for phase I and II clinical trials, where it is administered in the form of an extract or broccoli sprouts. The results of these studies are promising and indicate which SFN, in the future, may be considered as an anticancer drug. The situation is different for synthetic analogs of SFN, which have been described in this review, having ↑-more active than SFN; ↓-less active than SFN; ↔-similar active to SFN.
Based on Table 13, it can be seen that replacing the methyl group in sulfinyl moiety in SFN with structurally diverse fluorine ((S)-75, 80d), heterocyclic (84d), or aliphatic (e.g., isobutyl) (152) substituents result in increases of the biological activities of analogs of SFN on different cancer cell lines, such as Malme-3M, MCF-7, or A549. For phosphorus analogs of SFN (P-SFN), the presence of phosphine oxide moiety with methyl substituents (191) does not affect biological activity. However, replacement of the methyl sulfinyl group by phosphonate moiety with aliphatic (298) or aromatic (407) substituents or phosphinate moiety (372) results in an increase in the anticancer activity on LoVo and LoVo/DX cancer cell lines. Carbonyl or amide analogs of SFN (419 and 455) can by characterized by biological activities similar to SFN, but on the other hand, for some analogs (e.g., 470e), biological activity could be higher than for SFN. The presence of the second isothiocyanato group (484) also results in an increase in the biological activity on LoVo and LoVo/DX cancer cell lines.

Conclusions
There are many examples describing the synthesis of SFN and its natural or synthetic difunctional analogues, with methyl sulfinyl group replaced by sulfinyl, sulfanyl, sulfonyl, phosphonate, phosphinate, phosphine oxide, carbonyl, ester, amide, ether, or a second isothiocyanate group. The most common strategies involve the use of thiophosgene as a thiocarbonyl transfer reagent, utilization of a carbon disulfide/desulfurizing agent system (dithiocarbamates approach), or use of the tandem Staudinger/aza-Wittig reaction (triphenylphosphine/carbon disulfide system). In the first two approaches, primary amines are utilized as substrates; the last comprises azides as starting materials. The choice of method depends on the availability of starting substrates and sensitivity of functional groups present in the substrates to the reaction conditions. Several ITCs are characterized by high biological activity (anticancer or antibacterial), usually higher than parental SFN. Which modifications of the structure of SFN seem to have important impacts on the biological activities of the new analogues of SFN.
As SFN is biologically active, natural ITC is found in cruciferous vegetables, and it is non-toxic-it has been selected for phase I and II clinical trials, where it is administered in the form of an extract or broccoli sprouts. The results of these studies are promising and indicate which SFN, in the future, may be considered as an anticancer drug. The situation is different for synthetic analogs of SFN, which have been described in this review, having sulfinyl, sulfanyl, sulfonyl, phosphonate, phosphinate, phosphine oxide, carbonyl, ester, amide, ether, or a second isothiocyanate group. The vast majority of these compounds have only been tested for anticancer or antibacterial activity in vitro, and a few analogs, additionally in vivo, on mice or Zebrafish. None of the synthetic analogs of SFN described in this review qualified for clinical trials. This is likely due to the toxicity of these compounds in higher doses, often seen in in vitro or in vivo studies. SFN, which is a natural product, does not show toxicity, even in higher doses. Another reason is the financial constraints of many research teams.
One interesting solution would be to synthesize a conjugate-a combination of SFN and a drug or peptide, with anticancer properties, characterized by no (or low) toxicity. This conjugate could separate, in cancer cells, on two drugs, causing increased anticancer activity.
Funding: This research received no external funding.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available upon request from the corresponding author.