Antioxidant and Antitumor Activities of Newly Synthesized Hesperetin Derivatives

Hesperetin is a class of natural products with a wide range of sources and remarkable biological activities. In this study, we described the synthesis of a series of novel hesperetin derivatives and evaluated the in vitro antioxidant and antitumor activity of these compounds. Eleven novel compounds were synthesized in moderate yields. The compounds synthesized in this work exhibited antioxidant activities against DPPH and ABTS free radicals in a dose-dependent manner. Among them, compound 3f had the best antioxidant activity, with IC50 of 1.2 μM and 24 μM for DPPH and ABTS, respectively. The antitumor activity of the compounds against human cancer cell lines, such as breast MCF-7, liver HepG2, and cervical Hela, was determined by a standard 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Three compounds had moderate IC50 values. Interestingly, compound 3f had better biological activity than hesperetin, which matches the prediction by Maestro from Schrödinger. Therefore, the new hesperidin derivative is a promising drug for the treatment of cancer due to its effective antitumor activity. The results also suggested that the antitumor activities of hesperetin derivatives may be related to their antioxidant activities.


Introduction
Flavonoid compounds are a kind of natural product with a wide range of natural existence. Hesperidin and hesperetin are the main active ingredients of citrus fruits, and hesperetin is a natural product worthy of study in citrus [1]. Application research of the natural product hesperetin is a hot frontier in related neighborhoods [2]. Hesperetin has a variety of pharmacological activities, such as anti-inflammatory [3], antioxidant [4,5], antibacterial [6,7], anticancer [8,9], anti-hepatic fibrosis [10], and immune regulation [11]. However, since hesperidin and hesperetin are easily decomposed and absorbed in the gastrointestinal tract after oral administration [12], the bioavailability of hesperidin and hesperetin is low, and the activity is general [13]. Therefore, the structural modification of hesperetin to better achieve the biologic activity of hesperetin is worthy of study.
Previously, many studies have found that hesperetin has antitumor activity because it elicits apoptosis in many cancer cells [14][15][16]. Hesperetin has multiple phenol structures that may be replaced by all kinds of functional groups in the benzene ring. Existing research has revealed that structurally modified 7,3 -dimethoxy hesperetin induces apoptosis [17]. The hydrogen bond between 5-hydroxy of hesperetin and 4-ketochromate was also found to enhance the application ability of hesperetin [18]. In addition, in a study of improving anticancer activity, it was also found that the structurally modified hesperetin at 4 -and 7-positions had better anticancer activity [19]. It can be concluded that the biological activity depends to a large extent on the structural characteristics; however, its 6-position derivatives have rarely been reported with antitumor activities. It is well known that the changes in the biological activity of compounds are caused by minor structural modifications of the bulk structure of compounds. Because many active compounds are similar to alkaloids in Chinese and Western medicine, the introduction of bases to compounds can improve their biological activity [20]. As we all know, benzyl amine derivatives widely exist in natural products, and they are essential for medical compounds [21]. Thus, we want to bind the benzyl amines to the 6-position of hesperetin. By molecular docking, we found that protein molecules can provide space for the splicing of two structural frameworks. By optimizing the reaction temperature and reaction time, a higher C-6 regioselectivity can be obtained [22]. Moreover, hesperetin, as a flavonoid, has been proven to be one of the sources of natural antioxidants. Structural modification of hesperetin to find compounds with more antioxidant capacity for application in food and pharmaceutical industries will have more interesting application foregrounds. Hesperetin can be used as natural antioxidants in the food and medical industries with broad application prospects [23,24]. Therefore, in this work, we report the synthesis of new hesperetin derivatives and evaluate the antioxidant and antitumor activities of these compounds. Our team looks forward to finding compounds that can simultaneously enhance a variety of biological activities in these hesperetin derivatives and to promoting further application development.

Chemistry
Hesperetin derivatives (3a-3k) are obtained by the steps described in Scheme 1. Firstly, hesperidin 1 was hydrolyzed with sulfuric acid in methanol to obtain hesperidin 2, and then the target compound was synthesized. The synthesis strategy of hesperetin derivatives is the benzylation of hesperetin with benzaldehyde and amine. In our experiment, hesperetin derivatives were synthesized at the C-6 position, which were amine benzyl derivatives (3a-3k), by an electrophilic substitution reaction in methanol at 40 • C.
The scope of this three-component reaction was investigated. As presented in Scheme 1, a wide range of aliphatic and aromatic amines were tolerated to furnish the substituted hesperetin 2. Straight-chain, cyclic, primary, and secondary amines were all reactive in this multicomponent reaction (3a-3d). The secondary aryl alkyl amine afforded 3e in 55% yield. Benzyl amines reacted efficiently to render the products 3f and 3g in 47% and 66% yields, respectively. Aromatic amines bearing either an electron-rich or an electron-poor group on the benzene ring were compatible and delivered the products in moderate yields (3j, 3k). It can be clearly seen from the 1 H-MNR of 3a that the proton at the C-6 position of hesperetin disappeared. The structure of the ultimate product was determined by 1 H-NMR, 13 C-NMR, and HRMS spectral characterization data.

Antioxidant Activities Research In Vitro
It is reported that natural plant products can scavenge free radicals that result in cell death, histological damage, and chronic conditions development [25]. Hesperetin has antioxidant activity. Restraining or eliminating the priming or reproduction of superfluous active substances can postpone or inhibit cell injury caused by physiological oxidants and decrease the risk of potential human health influence related to oxidative stress or free radicals [23,26]. It is significant to measure the antioxidant activity of hesperetin derivatives in the exploration of their potential applications in the food, pharmaceutical, or cosmetics industries. For different antioxidant mechanisms, many methods for testing the antioxidant activity of compounds have been developed [24]. In this study, DPPH and ABTS testing methods [27] were selected for antioxidant research. The mechanisms of both methods are related to electron transport and the revivification of colored oxygenant; this makes it easy to observe the antioxidant activity of the tested compounds through the change of absorbance. Vitamin C (V C ) was a positive control. IC 50 values were computed using IBM SPSS Statistics 25.0.
As shown in Table 1, vitamin C as a positive control showed strong antioxidant activity. Obviously, compared with hesperetin (70 µM and 276 µM in DPPH and ABTS assays, respectively), the IC 50 of all hesperetin derivatives were significantly lower, and some compounds were lower than those of V C (59 µM and 236 µM in DPPH and ABTS assays, respectively). It is gratifying that compound 3f showed the best antioxidant capacity, the IC 50 value in the DPPH method was 1.2 µM, and the IC 50 value in the ABTS method was 24 µM. It can be seen that the antioxidant capacity of the compounds was markedly improved by the structural modification of hesperetin. The scope of this three-component reaction was investigated. As presented in Scheme 1, a wide range of aliphatic and aromatic amines were tolerated to furnish the substituted hesperetin 2. Straight-chain, cyclic, primary, and secondary amines were all reactive in this multicomponent reaction (3a-3d). The secondary aryl alkyl amine afforded 3e in 55% yield. Benzyl amines reacted efficiently to render the products 3f and 3g in 47% and 66% yields, respectively. Aromatic amines bearing either an electron-rich or an electron-poor group on the benzene ring were compatible and delivered the products in moderate yields (3j, 3k). It can be clearly seen from the 1 H-MNR of 3a that the proton at the C-6 position Scheme 1. Substrate scope of aminobenzene methylation of hesperetin 2 (Reaction conditions: hesperetin 2 (2 mmol), benzaldehyde (2 mmol), amine (3 mmol), and methanol (40 mL) at 40°C for 8 h. Isolated yields.

Molecular Docking
We simulated the molecular docking between hesperetin and carcinogenic protein molecules to intuitively describe the reason why this structural modification improves the antitumor ability. As a crucial molecule of HPV16 carcinogenesis, the E6 protein is an important transformation protein for cell proliferation [28]. The outcomes are demonstrated in Figure 1. The docking results indicate hesperetin 2 interacted with E6 protein (Figure 1a). Compound 3f interacts with the E6 protein has a better Glide score of −5.061 than hesperetin 2 (Figure 1b), which is −4.639.

Antitumor Activity Research In Vitro
To explore the anticancer activity of the synthetic compounds, our team studied the antitumor activity of hesperetin and its derivatives in MCF-7, HepG2, and HeLa cells. Compound concentration was set at 50 µM for screening; screening found that several hesperetin derivatives showed strong antiproliferative activity, as shown in Table 2. Seven compounds 2, 3a, 3e, 3f, 3g, 3i, and 3k, exhibited preferable antiproliferative activity against one or more cancer cells (inhibition rates were greater than 50%), which could be further used for the determination of IC 50 values.

Antitumor Activity Research In Vitro
To explore the anticancer activity of the synthetic compounds, our team studied the antitumor activity of hesperetin and its derivatives in MCF-7, HepG2, and HeLa cells. Compound concentration was set at 50 μM for screening; screening found that several hesperetin derivatives showed strong antiproliferative activity, as shown in Table 2. Seven compounds 2, 3a, 3e, 3f, 3g, 3i, and 3k, exhibited preferable antiproliferative activity against one or more cancer cells (inhibition rates were greater than 50%), which could be further used for the determination of IC50 values.
As shown in Table 3, through the calculation of IC 50 , with cisplatin as the positive control, the IC 50 values of the screened compounds on MCF-7, HepG2, and HeLa cells were higher than those of cisplatin, and the cytotoxicity of the compounds was lower than that of cisplatin. Furthermore, compared with hesperidin, compounds 3e, 3f, and 3k, through structural modification, showed significant antiproliferative activities against the three cancer cells. Among them, compound 3f had the strongest antiproliferative activity, and its IC 50 values for the three cancer cells were 5.3 µM, 8.8 µM, and 8.6 µM, respectively.
As shown in Figure S1 in Supplementary Materials, the inhibition rate of hesperetin 2 and its derivatives 3e, 3f, 3k, and cisplatin on three kinds of cancer cells, MCF-7, HepG2, and HeLa cells, varies with the strength of the solution. With different strength of solutions (0.01, 0.1, 1, 10, 50 µM) of hesperetin 2 and its derivatives 3e, 3f, 3k and cisplatin on MCF-7, HepG2, and HeLa cells, the inhibition rate of cancer cells increases with the increase in concentration, and the differences of different compounds are compared. Compared with hesperetin, its derivatives have a stronger inhibitory effect on three kinds of cancer cells in a dose-dependent manner.

Discussion
In the reaction in our work, the benzaldehyde protonates to the carbonyl group, and the amine compound undergoes nucleophilic addition to the carbonyl group, deprotonation, electron transfer on the nitrogen, and water departure to obtain an electrophilic imine ion intermediate. The intermediate attacks the active hydrogen at the C-6 position of the hesperetin A-ring, loses the proton, and obtains the final product. This method is widely used in the synthesis and modification of our lead compounds.
As shown in Table 1, it is worth noting that almost all hesperetin derivatives have stronger activities than hesperetin in terms of antioxidative capacity appraised by DPPH and ABTS methods. Compared with V C , the activity of compounds evaluated by the DPPH method was almost better than that of V C , and the activity of only half of hesperetin derivatives was stronger than that of V C evaluated by the ABTS method. All compounds cleared free radicals in a dose-dependent manner. This confirmed that antioxidant activity displayed by structurally modified derivatives was greatly improved in vitro. Hesperetin derivatives are expected to become antioxidants.
As shown in Table 2, it is worth noting that for HeLa cancer cell lines, almost all hesperetin derivatives are more potent than hesperetin, while for HepG2 cancer cell lines, only half of hesperetin derivatives have stronger activity than compounds 2. Figure S1 in the Supplementary Materials confirms that in vitro, the antitumor of the structurally modified derivatives is greatly improved. Interestingly, the inhibitory ability displayed by derivatives on MCF-7 and HeLa is better than HepG2, indicating that MCF-7 and HeLa are more sensitive to derivatives than HepG2. In a word, the structural modification of this series of hesperetin derivatives is successful. The results also suggested that the antitumor activities of hesperetin derivatives may be related to their antioxidant activities [29].
In order to explore the binding space between E6 protein and the compounds, we initially introduced some aliphatic chains at the 6-position of the structure, so we synthesized 3a, 3b, 3c, and 3d. The activities of 3a, 3b, 3c, and 3d are similar to compound 2, suggesting that their antiproliferative activity is not affected by fatty amines. We noticed that the introduction of the aliphatic chain has little effect on the potency, so we introduced a larger benzene ring and synthesized the compounds 3e, 3f, and 3g, and found that the activity was improved. Subsequently, we synthesized the derivatives with two aromatic rings as 3h, and found that the activity decreased. So, we kept the strategy of introducing an aromatic ring at the 6-position, changed the position of substituents of the benzene ring, and found that keeping a group at the para position of the benzene ring was beneficial to the potency. For example, compound 3k has good inhibitory activity. As shown in Figure 2, we draw a schematic diagram of the structure-activity relationship of compound 3f, according to our designed experimental results. This confirms that in vitro antitumor activities displayed by structurally modified derivatives are greatly improved. Interestingly, the inhibitory ability displayed by derivatives on MCF-7 and HeLa is better than HepG2, indicating that MCF-7 and HeLa are more sensitive to derivatives than HepG2. Table 1, it is worth noting that almost all hesperetin derivatives have stronger activities than hesperetin in terms of antioxidative capacity appraised by DPPH and ABTS methods. Compared with VC, the activity of compounds evaluated by the DPPH method was almost better than that of VC, and the activity of only half of hesperetin derivatives was stronger than that of VC evaluated by the ABTS method. All compounds cleared free radicals in a dose-dependent manner. This confirmed that antioxidant activity displayed by structurally modified derivatives was greatly improved in vitro. Hesperetin derivatives are expected to become antioxidants.

As shown in
As shown in Table 2, it is worth noting that for HeLa cancer cell lines, almost all hesperetin derivatives are more potent than hesperetin, while for HepG2 cancer cell lines, only half of hesperetin derivatives have stronger activity than compounds 2. Figure S1 in the Supplementary Materials confirms that in vitro, the antitumor of the structurally modified derivatives is greatly improved. Interestingly, the inhibitory ability displayed by derivatives on MCF-7 and HeLa is better than HepG2, indicating that MCF-7 and HeLa are more sensitive to derivatives than HepG2. In a word, the structural modification of this series of hesperetin derivatives is successful. The results also suggested that the antitumor activities of hesperetin derivatives may be related to their antioxidant activities [29].
In order to explore the binding space between E6 protein and the compounds, we initially introduced some aliphatic chains at the 6-position of the structure, so we synthesized 3a, 3b, 3c, and 3d. The activities of 3a, 3b, 3c, and 3d are similar to compound 2, suggesting that their antiproliferative activity is not affected by fatty amines. We noticed that the introduction of the aliphatic chain has little effect on the potency, so we introduced a larger benzene ring and synthesized the compounds 3e, 3f, and 3g, and found that the activity was improved. Subsequently, we synthesized the derivatives with two aromatic rings as 3h, and found that the activity decreased. So, we kept the strategy of introducing an aromatic ring at the 6-position, changed the position of substituents of the benzene ring, and found that keeping a group at the para position of the benzene ring was beneficial to the potency. For example, compound 3k has good inhibitory activity. As shown in Figure 2, we draw a schematic diagram of the structure-activity relationship of compound 3f, according to our designed experimental results. This confirms that in vitro antitumor activities displayed by structurally modified derivatives are greatly improved. Interestingly, the inhibitory ability displayed by derivatives on MCF-7 and HeLa is better than HepG2, indicating that MCF-7 and HeLa are more sensitive to derivatives than HepG2. The molecular docking in our work also indicates that hesperetin 2 has an obvious binding affinity to E6 protein, and hesperetin 2 binds within the active area of E6 protein. This means that structural modifications can be made to improve binding affinity. The molecular docking in our work also indicates that hesperetin 2 has an obvious binding affinity to E6 protein, and hesperetin 2 binds within the active area of E6 protein. This means that structural modifications can be made to improve binding affinity. Furthermore, we noticed that between the phenyl groups of Trp2 and 3f, there is a typical π-π conjugation. The amino group established hydrogen bonds with the Ser103 and the Afc105. These interactions explain why compound 3f has more potency than other compounds. The docking results show that this is consistent with the activity of compound 3f.

Synthesis of Hesperetin (2)
Hydrolysis of hesperidin to hesperetin is generally easy to implement. We used about 3.5 g. 5.73 mmol of hesperidin 1, adding 280 mL methanol and 10 mL 96% H 2 SO 4 while stirring. The reaction solution was set to heating temperature 70 • C and refluxed for 7.5 h. After the reaction stopped heating, the reaction mixture was cooled to room temperature. Then, the reaction solution was extracted and poured into EtOAc about 300 mL. The organic phase was washed with water (3 × 400 mL) until the lower aqueous phase was colorless and transparent, and then the upper organic phase was concentrated. Then, acetone was used to dissolve the residue about 70 mL and drop it into the hot 1% AcOH aqueous solution of about 700 mL. The heating temperature was set at 70 • C, and the stirring was 1.5. After solution cooling, filtration, and drying, hesperetin was obtained.

Synthesis of Hesperetin Derivatives (3a-3k)
The synthesis strategy of hesperetin derivatives is the benzylation of hesperetin with benzaldehyde and amine. Briefly, 0.604 g of hesperetin (2 mmol) was taken in the reaction vessel, 40 mL of methanol was added to dissolve it. Then, we added 204 µL of benzaldehyde (2 mmo1, 1.0 equivalent) and 1.1 equivalent of the amine-containing compound; the reaction temperature was 40 • C. The reaction was completed after stirring for 8 h; then, the reaction was monitored by TLC. The reaction mixture was concentrated and evaporated in vacuum to obtain crude products. The reaction mixture was concentrated and evaporated in vacuum and extracted with ethyl acetate-water to a colorless and transparent aqueous phase. The organic layer of ethyl acetate was retained and dried with anhydrous Na 2 SO 4 for 4 h. The mixture was concentrated under reduced pressure to obtainrough stuff, and the rough stuff was purified by silica gel column chromatography (EtOAc/PE, 3:1→1:1) to obtain compounds (3a-3k).

Structure Characterization of Hesperetin and Hesperetin Derivatives (3a-3k)
The 1 H-NMR and 13 C-NMR spectra were obtained in CDCl 3 on a 400 and 100 MHz NMR spectrometer (Bruker, Karlsruhe, Germany). The chemical shifts were referenced to signals at 7.26 and 77.0 ppm, respectively, and chloroform was used as the solvent with TMS as the internal standard. HRMS data were collected from a high-resolution mass spectrometer (QTOF-LCMS-9030, SHIMADZU) (Kyoto, Japan).

Cell Culture
Human MCF-7 cancer cells, HepG2 cancer cells, and Hela cancer cells were collected from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China). The frozen Human MCF-7 cancer cells, HepG2 cancer cells, and Hela cancer cells were quickly removed from the liquid nitrogen tank and shaken in a 37 • C water bath. After all the substances in the freezer were completely melted, the solution in the freezer was slowly dropped into a 10 mL centrifuge tube. The centrifuge tube had an appropriate amount of fresh medium (Gibco-BRL, Gaithersburg, MD, USA) pre-added to seal the membrane. At room temperature, we centrifuged at 1000× g for 5 min. We removed supernatant, suspended in 2 mL fresh culture RPMI1640, and transferred it to sterile culture bottle. We added the culture medium into 5-6 mL and cultured at 37 • C, 5% CO 2 incubator. We replaced fresh medium 24 h later.

MTT Assay
This study is of the cytotoxicity of hesperetin and hesperetin derivatives (3a-3k) to MCF-7, HepG2, and Hela cell lines by methyl thiazolyl tetrazolium (MTT) assays. First of all, MCF-7, HepG2, and Hela cell lines were seeded into a 96-well cell culture plate. The seed concentration was about 1 × 10 4 /hole; then, the cell lines were cultured at 37 • C under 5% Molecules 2022, 27, 879 8 of 11 CO 2 for 24 h. After that, the concentration of hesperetin and hesperetin derivatives (3a-3k) (0, 0.01, 0.1, 1, 10, 50 µM) were added in these wells, respectively. After being incubated for another 48 h at the same conditions, the MTT solutions (10 µL, 0.5 mg/mL) were added to these wells. Then, cells were incubated for 4 h. The medium was removed, and 100 µL dimethyl sulfoxide was added to dissolve the formazan crystals. The OD570 was measured by an ELx800 microplate reader (Bio-Tek, Winooski, VT, USA) with subtraction of background absorbance. The cell viability was computed using following equation: where A C is the absorbance of the control, and A S is the absorbance of dyes. All samples were analyzed in triplicate, and the results are expressed as mean ± standard deviation. The cytotoxicity of hesperetin and hesperetin derivatives (3a-3k) was expressed as an IC 50 , defined as the concentration causing a 50% reduction in cell growth compared with untreated cells.

Molecular Docking
Docking study of hesperetin and hesperetin derivative 3f was carried out by Schrödinger's Maestro and PyMOL programs. The crystal structure of HPV16 protein E6 (ID: 4XR8) was retrieved for this work from the protein database (PDB). The protein molecules were pretreated and stored in. pdbqt format. The B3LYP/6-311G ** level of density functional theory (DFT) was selected to predict the simulation, and the ligand molecules were optimized by Gaussian 03 software. In addition, ligands (isomers) such as hesperetin and compound 3f were also saved in pdbqt format. In the docking studies using software, the grid box was set to 60 × 60 × 60 dimensions to select the protein molecules and ligand molecules, and the La-marckian genetic algorithm was used to find the global optimal conformation. Finally, the interaction between protein and ligand was evaluated by Glide score.

DPPH Antioxidant Activity Assay
In this study, stable and convenient free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to determine the free-radical-scavenging activity of hesperetin and its derivatives in this study. The experimental samples were diluted with ethanol into different concentrations of sample solution, and then the accurate amount of 0.5 mL sample solution and 2.5 mL 60 µM DPPH solution were dissolved in ethanol. The mixed solution was oscillated vigorously in the dark for 1 h, and the absorbance of the reaction solution was measured by UV spectrophotometer at 517 nm. V C was positive control in this experiment. The expression formula of DPPH scavenging activity is as follows: where A C was the absorbance of negative control DPPH solution and As was the absorbance of 0.5 mL sample solution and 2.5 mL DPPH solution mixed solution. All the experimental samples were analyzed in three copies, and the results were expressed as mean ± standard deviation. The scavenging activity was expressed as 50% inhibitory concentration (IC 50 ), namely, the sample concentration of 50% DPPH free radical scavenging after incubation.

ABTS Antioxidant Activity Assay
The free-radical-scavenging ability of hesperetin and its derivatives was evaluated by stable and convenient ABTS method. The experimental samples were diluted with ethanol in turn into different concentrations of sample solutions, and then accurate amount was configured with 7 mM ABTS solution and 2.45 mM potassium persulfate solution with deionized water to produce ABTS radicals at room temperature in dark environment for 16 h. The absorbance of the accurate volume of ABTS solution diluted with ethanol to 734 nm was 0.70 ± 0.05. The diluted ABTS solution (2 mL) was mixed with 100 µL of the experimental sample, and the mixture was kept in dark at room temperature for 6 min. The absorbance of the mixed solution was measured by UV spectrophotometer at 734 nm. V C was used as the positive control in this experiment. The expression formula of ABTS scavenging activity is as follows: where A C is the absorbance of the ABTS solution as the control and A S is the absorbance of the ABTS solution after adding the sample. The IC 50 was computed from the graph of scavenging percentage against concentration using IBM SPSS Statistics 25.0. The results are expressed as mean ± standard deviation of three experiments.

Statistical Analysis
All experiments were performed at least in triplicate, and each experiment was independently performed at least 3 times. The graphical presentations were performed using Origin 2018 64 Bit. Data were presented as the mean ± standard and were analyzed using SPSS 25.0 software (Chicago, IL, USA). Analysis of variance (ANOVA) for samples was determined by Tukey's multiple range tests (p < 0.05).

Conclusions
In summary, a series of aminobenzylated hesperetin derivatives 3a-3k were successfully synthesized, and their antioxidant activity and antiproliferative activity were evaluated. In this series, the antioxidant activity of almost all compounds was higher than that of hesperetin. Compound 3f has the strongest antioxidant activity, and all compounds scavenged DPPH and ABTS free radicals in a dose-dependent manner. Meanwhile, the antiproliferative activity against three different cancer cells on MCF-7 cells, HepG2 cells, and HeLa cells of all the compounds is higher than that of hesperetin. Compound 3f also has the strongest antiproliferative activity. In a molecular-docking study, compared with hesperetin, compound 3f has a better interaction with the E6 protein, and the Glide score is also better. The results signified that the structural modification of hesperetin in the successful evaluation of derivatives was improved, further research is underway, and the results will be disclosed in due course.
Supplementary Materials: The following are available online, Section A: General Method; Section B: General procedure for the synthesis of hesperetin derivatives; Section C: Analytical data; Section D: HRMS of Compounds; Section E: Antioxidant Activities Research; Section F: Anti-tumor activity assays; Section G: 1 H-and 13 C-NMR Spectra of Compounds and Figure S1. Cytotoxicity evaluation of hesperetin 2 and derivatives 3e, 3f, 3k and Cisplatin on MCF-7 (a); HepG2 (b); HeLa (c). (The treatment time used in cytotoxicity studies is 48 h) (PDF).
Author Contributions: G.Z. implemented the experiment and wrote the paper; G.Z. conducted the experiment; J.S. and G.L. checked the paper; Z.L., J.W., C.L., and S.H. assisted in the experiments; Z.C., P.L. and L.L. gave advice; G.L. and P.L. provided laboratory equipment; G.Z. and J.S. designed the experiment. All authors have read and agreed to the published version of the manuscript.