Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots

Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.


Introduction
Argylia radiata (L.) D. Don. is a herbaceous perennial plant that is part of the Bignoniaceae family. This species is native to Chile, dwelling in the Atacama Desert as a representative specie of the Flowering Desert, a random phenomenon caused by winter rains associated with the Child Current [1]. The Argylia plant sprouts and flourishes only when enough water is available. In the remaining time, they leave a massive tuberous root

Phenolic Compounds Analysis
The presence of polyphenols in plants is determined by genetic and environmental factors. They can interact directly with other cellular components, regulating cell processes as mitosis, cell elongation, senescence and cellular die [21]. In addition, they play an essential role in plant development under stress conditions due to its inherent antioxidant capacity. In the present work, different extraction solvents are used to isolate phenolic compounds. Comparative determination of the total polyphenolics content (TPC) and flavonoid content (FC) in plant root and vitroplants was performed. The results of TPC and FC quantification are shown in Figure 2. It was determined that the highest amount of polyphenolic compounds was found in the WR and MC (69.3 and 68.1 mg GAE/100 g FW) extracts, respectively. The TPC of those extracts was significantly higher (p > 0.05) than determined for the remaining Argylia

Phenolic Compounds Analysis
The presence of polyphenols in plants is determined by genetic and environmental factors. They can interact directly with other cellular components, regulating cell processes as mitosis, cell elongation, senescence and cellular die [21]. In addition, they play an essential role in plant development under stress conditions due to its inherent antioxidant capacity. In the present work, different extraction solvents are used to isolate phenolic compounds. Comparative determination of the total polyphenolics content (TPC) and flavonoid content (FC) in plant root and vitroplants was performed. The results of TPC and FC quantification are shown in Figure 2.

Phenolic Compounds Analysis
The presence of polyphenols in plants is determined by genetic and environmental factors. They can interact directly with other cellular components, regulating cell processes as mitosis, cell elongation, senescence and cellular die [21]. In addition, they play an essential role in plant development under stress conditions due to its inherent antioxidant capacity. In the present work, different extraction solvents are used to isolate phenolic compounds. Comparative determination of the total polyphenolics content (TPC) and flavonoid content (FC) in plant root and vitroplants was performed. The results of TPC and FC quantification are shown in Figure 2.  It was determined that the highest amount of polyphenolic compounds was found in the WR and MC (69.3 and 68.1 mg GAE/100 g FW) extracts, respectively. The TPC of those extracts was significantly higher (p > 0.05) than determined for the remaining Argylia  It was determined that the highest amount of polyphenolic compounds was found in the WR and MC (69.3 and 68.1 mg GAE/100 g FW) extracts, respectively. The TPC of those extracts was significantly higher (p > 0.05) than determined for the remaining Argylia extracts. Likewise, it was observed that, depending on the extracting solvent, the TPC of C, P and R will vary while always maintaining a high content of TPC for methanolic extracts and the plant root starting material.
It is known that methanol is a good solvent for polyphenol extraction. In a recent study carried out in Argylia plant organs [12], we determine that the highest TPC was found in the plant leaves (1520 mg GAE/100 g FW), followed by the flowers and roots (70 mg GAE/100 g FW). It is worth mentioning that those extracts were made using methanol as a solvent. The TPC content obtained for MR and WR extracts from the present work were not significantly different among them and they were slightly lower than MC and WR samples, which is interesting.
General speaking, the TPC values from vitroplants are far from those observed in Argylia plant leaves and flowers collected in their natural habitat. Such differences can be due to the high stress at which those parts are exposed in nature, provoking secondary plant metabolism activations as one of their adaptive responses.
Larrazabal-Fuentes et al. [22] studied the extract of the Acantholippia deserticola (rica rica) plant, native to northern Chile. They determined a TPC value of 25 mg GAE/mL extract. A relationship with the TPC of Argylia was observed since these two plants share the same ecosystem.
In addition, Wong-Paz et al. [23] studied phenolic extraction from Mexican desert plants using an ethanolic mixture. They found TPC ranging from 70-332 mg GAE/100 g FW of Jatropha dioica, 52-310 mg GAE/100 g FW of Flourensia cernua, 112-350 mg GAE/100 g FW of Turnera diffusa and 390-1339 mg GAE/100 g FW of Eucalyptus camaldulensis. Results obtained for all Argylia methanolic extracts and WR extract were close to the TPC range from the first two plants, but only the EP treatment was close to Jatropha dioica extract polyphenolic content. However, the Argylia extract TPCs were 2-to 32-times lower than those determined in the other species.
This result is interesting because the TPC found in several of our extracts is similar to plants grown under desert high-stress conditions (UV, temperature, salinity etc.). Then, the micropropagation technique can be considered an interesting tool for producing polyphenolic extracts from scarce and slow-growing desert plants. Plant polyphenols have been suggested as a promising source of antimicrobial agents, either alone or combined with commercial antibiotics, to develop new antibiotic therapies [24].
In addition, the beneficial effects of polyphenol consumption on chronic health conditions, cardiovascular disease, type 2 diabetes, neurological impairment and certain risk factors have been reported [25,26]. The flavonoid content indicates that methanol and water, but not ethanol, are good solvents for flavonoid extraction. This could be due to the polarity of the solvents and the affinity of the flavonoids with them. Researchers observed a significantly (p > 0.05) higher FC in the MR sample (3.94 mg QE/100 g FW), followed by the WP, WR, MP and MC samples, in the same order. However, non-significant differences were found among all those extracts. The ethanolic extracts contained low amounts of flavonoids (<1 mg QE/100 g FW), and the ER sample was the one with the lowest significant (p > 0.05) FC values.
In [12], Morales-Tapia et al. studied the FC of A. radiata plants growing in their harsh natural habitat and observed that the FC content was higher in red flowers (43.5-187.6 mg QE/100 g FW) and leaves (126.6 mg QE/100 g FW) but lower in root pulp and cork (5.7 and 1.1 and mg QE/100 g FW). Those results agree with the present work, considering that the whole root sample was used for extract preparation. It is interesting to note that MP and WP vitro plant samples showed a similar concentration of flavonoids to the A. radiata tuberous root.
In addition, Sharma and Vig [27] determined the FC in different extracts of leaves from Parkinsonia aculeata, another desert plant. They found 1.3 mg R (rutin) E/100 g FW in methanolic extract and 0.6 mg RE/100 g FW water extract. In Ephedra chilensis, 2.3 mg QE/100 g DW was obtained in hexane, 9.5 mg QE/100 g DS in CH 2 Cl 2 and 0.5 mg QE/100 g DS in ETOH [28]. Considering the above results, it is confirmed that the A. radiata plant has a high content of flavonoids compared to other plants that coexist in a similar ecosystem, which could influence the antioxidant capacity. in Figure 3, a total of six polyphenols were identified among the samples. Syringic acid was the most frequent and abundant polyphenol in most extracts, followed by caffeic and coumaric acid, respectively. It is interesting to note that gallic acid was only determined in EP and EC samples, whereas MP and MR samples mostly contain coumaric acids. Syringic acid was present in roots, plants and callus tissue.

UPLC-MS/MS Analysis
Its presence could indicate a potential application of this extract since this polyphenol has shown antidiabetic activity [29], and antimicrobial activity inhibiting the growth of Escherichia coli was also reported [30]. Not present in all extracts but with high importance due to their biological activities is the polyphenol rutin. This flavonol glycoside has shown anti-inflammatory, antimicrobial and potent antioxidant activity as a scavenger of free radicals [31]. It is interesting to note that it was the most frequent metabolite found in wild A. radiata plants [12].

UPLC-MS/MS Analysis
A chromatographic analysis was performed to determine the chemical com of Argylia extracts obtained with different solvents. According to the results pre Figure 3, a total of six polyphenols were identified among the samples. Syringic the most frequent and abundant polyphenol in most extracts, followed by caffeic maric acid, respectively. It is interesting to note that gallic acid was only determin and EC samples, whereas MP and MR samples mostly contain coumaric acids. acid was present in roots, plants and callus tissue.
Its presence could indicate a potential application of this extract since this po has shown antidiabetic activity [29], and antimicrobial activity inhibiting the g Escherichia coli was also reported [30]. Not present in all extracts but with high im due to their biological activities is the polyphenol rutin. This flavonol glycoside ha anti-inflammatory, antimicrobial and potent antioxidant activity as a scavenge radicals [31]. It is interesting to note that it was the most frequent metabolite foun A. radiata plants [12].

Antioxidant Capacity Studies
The antioxidant capacity of Argylia extracts was evaluated towards the bio relevant ROS: peroxyl (ROO•), hydroxyl (OH•) radicals. Three different assays lected, including oxygen radical absorbance capacity-fluorescein (ORAC-FL), s ping assay and cellular antioxidant activity (CAA) to achieve the goals. Trolox ( droxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a vitamin E hydro-solubl tive, was used as the standard. All measurements were performed in triplicate values are expressed as the mean ± SD.

Evaluation of ORAC-FL
The antioxidant capacity of A. radiata extracts was determined through th FL method. This technique generates peroxyl and/or alkoxy radicals by therm 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) at 37 °C [32,3 radicals oxidize the fluorescein probe, which implies a decrease in the fluorescen sity of the probe as a function of time. Antioxidant compounds can delay oxidati probe through the transfer of hydrogen atoms [34][35][36].

Antioxidant Capacity Studies
The antioxidant capacity of Argylia extracts was evaluated towards the biologically relevant ROS: peroxyl (ROO·), hydroxyl (OH·) radicals. Three different assays were selected, including oxygen radical absorbance capacity-fluorescein (ORAC-FL), spin trapping assay and cellular antioxidant activity (CAA) to achieve the goals. Trolox ((±)-6-hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid), a vitamin E hydro-soluble derivative, was used as the standard. All measurements were performed in triplicate, and the values are expressed as the mean ± SD.

Evaluation of ORAC-FL
The antioxidant capacity of A. radiata extracts was determined through the ORAC-FL method. This technique generates peroxyl and/or alkoxy radicals by thermolysis of 2,2 -azobis (2-methylpropionamidine) dihydrochloride (AAPH) at 37 • C [32,33]. These radicals oxidize the fluorescein probe, which implies a decrease in the fluorescence intensity of the probe as a function of time. Antioxidant compounds can delay oxidation of the probe through the transfer of hydrogen atoms [34][35][36].
As the reaction rate between generated radicals and fluorescein is low, the antioxidant capacity studied by this methodology is related to the reaction's stoichiometry. However, the latter becomes relevant for pure compounds and not for complex mixtures of polyphenols, where it would only indicate the ability of an extract to scavenging oxygen-centered radicals [37]. Table 1 shows the antioxidant capacity for all extracts. The aqueous extracts showed the lowest ORAC antioxidant capacity. This may be due because only polyphenolic gly-coside compounds and low molecular weight hydroxylated organic acids were extracted in this solvent. For instance, the caffeic acid present in all the aqueous extracts has an ORAC-FL index 3.47-times higher than the reference antioxidant Trolox [36]. These results would indicate that, in aqueous extracts, the compound's combination would exert an antagonistic effect in relation to the antioxidant capacity analyzed by this methodology. On the other hand, ethanolic and methanolic extracts presented a lower content of polyphenolic compounds in relation to aqueous extracts; where a high content of syringic acid was identified in ethanolic extract with a lower ORAC-FL index than caffeic acid [38]. This difference may be due to the presence of other polyphenolic compounds, such as rutin and gallic acid. However, the observed differences in the phenolic composition between methanolic and ethanolic extracts do not explain the similar antioxidant capacity values studied by this methodology. The latter can be explained by additive, synergistic or antagonistic effects between the compounds present in the extracts.

Evaluation of OH· Scavenging by ESR
The spin trapping technique (ESR) evaluated hydroxyl elimination capacity. This technique consists of a reaction between a free radical with a diamagnetic molecule (spin trap), which generates a radical species with a longer half-life (spin adduct), which can be analyzed by electron spin resonance (ESR).
Hydroxyl radicals (half-life of 10 −9 s) [39] were generated by a non-catalytic Fenton reaction, and DMPO (5,5-dimethyl-1-pyrroline-N-oxide) was used as a spin trap. This allows the formation of radicals in basic medium without the use of Fe 2+ as a catalyst. Figure 4b shows the ESR spectra (as an example, only the spectra obtained for the ethanolic extracts are shown), with four lines corresponding to the interaction between the unpaired electron with the nuclei of the nitrogen and hydrogen atoms observed. The hyperfine coupling constants (DMPO-OH, aN = aH~14.76 G) obtained indicate the hydroxyl radical's trapping [39].
The extracts of Argylia vitroplant in the three solvents used decreased the intensity of the DMPO-OH spin adduct signal, which implies a decrease in the OH· radical concentration. The radical scavenging values (Table 1) indicate that the EP extract showed a higher hydroxyl radical scavenging capacity, followed by the MP extract. The antioxidant capacity studied by this methodology was correlated with that determined by ORAC-FL, since both methods involve the transfer of the hydrogen atom. Where the methanolic and ethanolic P extracts presented the highest hydroxyl radical removal capacity. higher hydroxyl radical scavenging capacity, followed by the MP extract. The antioxidant capacity studied by this methodology was correlated with that determined by ORAC-FL, since both methods involve the transfer of the hydrogen atom. Where the methanolic and ethanolic P extracts presented the highest hydroxyl radical removal capacity.

Evaluation of DPPH· Radical-Scavenging Activity by the EPR Method
A rapid, simple and economical method to determine antioxidant capacity involves the use of the free radical DPPH • . The test is used to detect the antioxidant capacity of fruits, vegetables, juices and extracts. DPPH assays are generally classified by a sequential mechanism of electron transfer with proton loss, in which the DPPH radical accepts an electron followed by proton transfer from antioxidant compounds.
Other authors postulated that a marginal mechanism of HAT (hydrogen atom transfer) may occur depending on the reaction medium and physicochemical properties of the compounds under study. The chemical determinations are usually made through a decrease of signal intensity in the spectrophotometry absorption spectrum. However, there could be several interferences with such results, such as the solvent used and the presence of undesired colored compounds in the samples [40].
In this work, to determine a decrease in the concentration of radical without interference, the antioxidant capacity determination was made using the EPR technique. The EPR spectrum of DPPH • shows a six-line pattern in which the depletion of signal intensity is proportional to the antioxidant capacity of compounds present in the extracts. In Figure  5, as an example, only the spectra obtained for the ethanolic extracts are shown. The time to reach the endpoint of DPPH • -EPR test depends on the medium and the properties of the molecules under study.
The test results are expressed as a function of the DPPH • decrease rate (dynamic approach) and characterize the reactivity of the compounds present in the extracts. Unlike expressing the amount of DPPH • removed, which indicates the stoichiometry of DPPH • reaction with donor H (the amount of active OH groups) [40][41][42][43]. The results of the DPPH • assay are presented in Table 1. All extracts showed radical removal capacity. The ethanolic and methanolic extracts exhibited the highest capacity to eliminate the radical DPPH • , with the extracts EP and MP as the ones with higher antioxidant capacities. The ability to eliminate the radical was the lowest for the aqueous extracts since DPPH has difficulty dissolving in water.
Larrazabal-Fuentes et al. [22] studied the antioxidant capacity of Acantholippia deserticola native to the Chilean desert, determining the capacity to eliminate the DPPH radical in a 50% ethanolic extract in water was 28.2 at 10 µg/mL. Those results corroborate our

Evaluation of DPPH· Radical-Scavenging Activity by the EPR Method
A rapid, simple and economical method to determine antioxidant capacity involves the use of the free radical DPPH·. The test is used to detect the antioxidant capacity of fruits, vegetables, juices and extracts. DPPH assays are generally classified by a sequential mechanism of electron transfer with proton loss, in which the DPPH radical accepts an electron followed by proton transfer from antioxidant compounds.
Other authors postulated that a marginal mechanism of HAT (hydrogen atom transfer) may occur depending on the reaction medium and physicochemical properties of the compounds under study. The chemical determinations are usually made through a decrease of signal intensity in the spectrophotometry absorption spectrum. However, there could be several interferences with such results, such as the solvent used and the presence of undesired colored compounds in the samples [40].
In this work, to determine a decrease in the concentration of radical without interference, the antioxidant capacity determination was made using the EPR technique. The EPR spectrum of DPPH· shows a six-line pattern in which the depletion of signal intensity is proportional to the antioxidant capacity of compounds present in the extracts. In Figure 5, as an example, only the spectra obtained for the ethanolic extracts are shown. The time to reach the endpoint of DPPH·-EPR test depends on the medium and the properties of the molecules under study.
The test results are expressed as a function of the DPPH· decrease rate (dynamic approach) and characterize the reactivity of the compounds present in the extracts. Unlike expressing the amount of DPPH· removed, which indicates the stoichiometry of DPPH· reaction with donor H (the amount of active OH groups) [40][41][42][43]. The results of the DPPH· assay are presented in Table 1. All extracts showed radical removal capacity. The ethanolic and methanolic extracts exhibited the highest capacity to eliminate the radical DPPH·, with the extracts EP and MP as the ones with higher antioxidant capacities. The ability to eliminate the radical was the lowest for the aqueous extracts since DPPH has difficulty dissolving in water.
Larrazabal-Fuentes et al. [22] studied the antioxidant capacity of Acantholippia deserticola native to the Chilean desert, determining the capacity to eliminate the DPPH radical in a 50% ethanolic extract in water was 28.2 at 10 µg/mL. Those results corroborate our findings that the water content in the extracts influences the antioxidant capacity of the compounds when measured by the DPPH methodology. Therefore, it is better to work with methanolic or ethanolic extracts. findings that the water content in the extracts influences the antioxidant capacity of the compounds when measured by the DPPH methodology. Therefore, it is better to work with methanolic or ethanolic extracts. The UPLC-MS/MS results indicated that EP extract showed the highest DPPH radical scavenging capacity since it contains antioxidant polyphenols, including rutin, gallic acid and syringic acid. In [44], Motlhanka et al. studied the antioxidant activity of several pure compounds, such as rutin. They determined that the rutin DPPH scavenging capacity was approximately 60% at 25 ppm. Gow-Chin, Y. et al., [45] studied the antioxidant activity of gallic acid against the radical DPPH, determining that the activity was 43.9% at 4.7 mM. In addition, the antioxidant activity of syringic acid was approximately 60% at 50 µM (Zhou, K. et al.) [46].
With those above, it can be concluded that there is a synergy between the compounds in the EP extract, since its antioxidant capacity value against the DPPH radical is 97.6%. For the MP extract, a synergy study cannot be done as it has only coumaric acid. It should be noted that the technique to determine the quenching of DPPH radicals by EPR is straightforward, since it makes a direct measurement of the radical in the presence of the antioxidant, while techniques, such as ORAC, DPPH, etc., are indirect measurement techniques because the results are obtained according to the variation of probes etc.

Evaluation of Cellular Antioxidant Activity (CAA)
The compound DCFH2-DA has been extensively used as a probe for the detection of oxidative stress that can reflect the overall oxidative status of the cell. The dye, DCFH2-DA, diffuses passively through the cellular membrane and is transformed into DCFH2a non-fluorescent compound that emits fluorescence when it is oxidized to 2,7-dichlorofluorescein (DCF) (λexcitation = 498 nm; λemission = 522 nm) by intracellular ROS-due to intracellular esterase activity. The AAPH is usually employed as the generator of peroxyl radicals. Therefore, it is generally accepted that hydrophilic antioxidants can interact more appropriately with the radicals generated by AAPH than hydrophobic antioxidants.
In Table 2, results from the cellular antioxidant activity of Argylia vitroplant extracts and root extracts are presented. It is worth mentioning that two concentrations of 1 and 10 µg/mL were evaluated, hoping that the antioxidant capacity increases as the concentration increases. In turn, if the concentration of 10 µg/mL is increased, the sensitivity of the method decreases. The extracts showed cellular antioxidant activity related to the type of solvent used in the extraction of antioxidant polyphenolic compounds and the vitro The UPLC-MS/MS results indicated that EP extract showed the highest DPPH radical scavenging capacity since it contains antioxidant polyphenols, including rutin, gallic acid and syringic acid. In [44], Motlhanka et al. studied the antioxidant activity of several pure compounds, such as rutin. They determined that the rutin DPPH scavenging capacity was approximately 60% at 25 ppm. Gow-Chin, Y. et al., [45] studied the antioxidant activity of gallic acid against the radical DPPH, determining that the activity was 43.9% at 4.7 mM. In addition, the antioxidant activity of syringic acid was approximately 60% at 50 µM (Zhou, K. et al.) [46].
With those above, it can be concluded that there is a synergy between the compounds in the EP extract, since its antioxidant capacity value against the DPPH radical is 97.6%. For the MP extract, a synergy study cannot be done as it has only coumaric acid. It should be noted that the technique to determine the quenching of DPPH radicals by EPR is straightforward, since it makes a direct measurement of the radical in the presence of the antioxidant, while techniques, such as ORAC, DPPH, etc., are indirect measurement techniques because the results are obtained according to the variation of probes etc.

Evaluation of Cellular Antioxidant Activity (CAA)
The compound DCFH 2 -DA has been extensively used as a probe for the detection of oxidative stress that can reflect the overall oxidative status of the cell. The dye, DCFH 2 -DA, diffuses passively through the cellular membrane and is transformed into DCFH 2 -a nonfluorescent compound that emits fluorescence when it is oxidized to 2,7-dichlorofluorescein (DCF) (λ excitation = 498 nm; λ emission = 522 nm) by intracellular ROS-due to intracellular esterase activity. The AAPH is usually employed as the generator of peroxyl radicals. Therefore, it is generally accepted that hydrophilic antioxidants can interact more appropriately with the radicals generated by AAPH than hydrophobic antioxidants.
In Table 2, results from the cellular antioxidant activity of Argylia vitroplant extracts and root extracts are presented. It is worth mentioning that two concentrations of 1 and 10 µg/mL were evaluated, hoping that the antioxidant capacity increases as the concentration increases. In turn, if the concentration of 10 µg/mL is increased, the sensitivity of the method decreases. The extracts showed cellular antioxidant activity related to the type of solvent used in the extraction of antioxidant polyphenolic compounds and the vitro plant part used. They also have antioxidant activity in a dose-dependent manner, increasing along with the extract concentration assayed.
In Table 2, the results of the cellular antioxidant activity of Argylia vitroplant extracts and root extracts are presented. It is worth mentioning that two concentrations of 1 and 10 µg/mL were evaluated in order to compare these results with those described in the literature. It was found that the extracts of P (stem and leaves) presented the highest cellular antioxidant activity in the three solvents used, as with the results obtained through ORAC-FL. The cellular antioxidant activity determined was higher than that obtained recently by Zúñiga-López et al. [47] for ethanolic extracts of Salvia hispanica L. (chia) leaves, which obtained a percentage CAA of 28 ± 2 and 13.2 ± 0.6 (evaluated at 10 µg/mL) for the white and black phenotypes, respectively. Those results points to Argylia vitroplant extracts as promising antioxidants for biotechnological applications.
On the other hand, the methanolic extracts of Argylia vitroplant showed the highest cellular antioxidant activity. The latter suggests that the polyphenolic compounds extracted in this solvent were more bioavailable for RAW 264.7 cells than the compounds extracted in ethanol and water. This outcome could also be attributed to the higher polyphenolic content found in the methanolic extracts or to their chemical composition. Further analysis to clarify this point is under progress in our group. In particular, the MP and MR extracts showed the highest cellular antioxidant activity at both concentrations. They displayed from 40-60-times higher activity at 1 µg/mL than the other ones. These results indicate that compounds extracted with methanol showed the highest effect as cellular antioxidants. On the other side, the antioxidant activity of ethanol and water extracts at 1 µg/mL concentration were similar, ranging from 17.6% to 23.4%. At 10 µg/mL, the antioxidant activity tendency is similar to at lower concentrations, except for the vitroplant water extract, which showed a high cellular antioxidant activity.
Polyphenolic extracts showing cellular antioxidant activity indicate that phenolic compounds can diffuse into the cell membrane and exert their activity into the cytoplasm. It is known that this antioxidant method accounts for aspects of uptake, metabolism and the location of antioxidant compounds within cells [48]. That makes it relevant and useful for natural product bioactivity evaluation.
Wolfe et al. [49] studied the cellular antioxidant capacity of pure compounds and different fruit extracts. These authors determined that quercetin was the best cellular antioxidant with 100% antioxidant activity, while caffeic acid and catechin have the lowest training of approximately 3% and 2%, respectively. Therefore, when evaluating the MP and MR extracts containing only coumaric acid, it could be concluded that coumaric acid has a better cellular antioxidant capacity when compared with catechin and caffeic acid but a lower antioxidant capacity compared with quercetin.
Likewise, in all the fruit extracts evaluated, the blueberry extract had the highest antioxidant capacity 8.70 µM quercetin equivalent (QE)/100 µmol of total phenols. In contrast, the green grape extract had the lowest activity 1.04 µM of QE/100 µmol of total phenols. Therefore, when comparing with the antioxidant capacity obtained by the MP and MR extracts, they observed that the extracts were good antioxidants compared to the fruit extracts.
All those results confirm Argylia vitroplant extracts' potential application as antioxidant compounds for different in vivo applications. In addition, these results demonstrate the usefulness of the tissue culture technique to obtain plant biomass at a large scale. Moreover, its relevance as a tool for obtaining interesting content of phytochemicals from extremophiles and scarcely available plant biomass in short times are highlighted.

Obtaining of Plant Material
In 2017, herbaceous shoots and tuberous storage roots of Argylia radiata were collected from Bahía Inglesa, Caldera, Región de Atacama, Chile (27 • 07 32 S; 70 • 48 53 W). The sprouts were protected with wet paper towels inside plastic bags during their transport, and they were kept at 4 to 7 • C until they were initiated while the roots were put on mesh and stored at room temperature.
The in vitro lines were obtained according to the described procedure by Morales [17]. The shoots were sterilized by dipping in a fungicide solution (Captan 20 g/L) for 30 min. After that, they were passed through an ethanol solution at 70% (v/v) and washed in a solution of commercial bleach at 20% (v/v) for 20 min. After, the material was rinsed three times with sterile water. Finally, nodal sections were planted in Murashige & Skoog medium [50], supplemented with 30 g/L of sucrose, 6.5 g/L of agar. Explants were kept in a growth chamber at 23 to 25 • C and 16 light hours. After six weeks, the new sprouts were isolated to a fresh medium. To obtain callus tissue, vitroplants of Argylia were cultivated for 5 weeks in growth media enriched with 0.5 mg/L of 6-Benzylaminopurine (BAP).

Plant Extract Preparation
The A. radiata vitroplants obtained ( Figure 1) were separated in callus (C) and plant (P) for polyphenol analysis. At the same time, Argylia tuberous roots, collected in the Atacama Desert, were obtained. Samples (1 g) were used to extract preparation with four different solvents: methanol (M), ethanol (E) and water (W). The solid:liquid ratio used in all cases was 1:10, and the extractions were performed at room temperature for 3 h under orbital shaking. Then, the extracts were centrifuged, and the liquid phases were stored at −20 • C for further analysis.

Total Phenolic Determination
The total phenolics content (TP) was determined according to the Folin Ciocalteu (FC) method. A 500 µL aliquot was mixed with 2.5 mL of the FC reagent 1:10 (v/v) and 2 mL of a solution of Na 2 CO 3 (75 g/L) and incubated 60 min at room temperature. The absorbance of the resulting blue solution was measured at 760 nm using an Agilent 8453 UV-visible spectrophotometer (Palo Alto, CA, USA). The calibration curve was performed using gallic acid, and the results are expressed as milligrams of gallic acid (GA) equivalents per gram.

Flavonoids Determination
The flavonoid content was estimated according to the aluminum chloride method [51]. A total of 500 µL of the extract was mixed with 500 µL of 2% AlCl 3 in methanol and incubated 60 min at room temperature. The absorbance was measured at 420 nm using an Agilent 8453 UV-visible spectrophotometer (Palo Alto). The total flavonoid contents were calculated as milligrams of quercetin equivalents per gram from a calibration curve.

UPLC-MS/MS Determination
An ABSciex triple Quad 4500 mass spectrometer equipped with an electrospray (Tur-boV) interface coupled to an Eksigent Ekspert Ultra LC100 with Ekspert Ultra LC100-XL autosampler system (AB/Sciex Concord, Concord, ON, Canada) was used for the determination of selected polyphenols. The chromatographic separation was carried out using a gradient elution of 0.1% formic acid in water (A) and methanol (B) as the mobile phase. The injection volume was 10 µL, and the flow rate was kept at 0.5 mL/min. A LiChrospher 100 RP-18 end-capped column (125 mm × 4 mm id, 5 µm) (Merck, Darmstadt, Germany) was used with a controlled temperature of 40 • C. Mass spectrometry analysis was operated in negative mode; the declustering potential (DP) and collision energy (CE) were optimized for each analyte. Compounds were identified by comparing MS/MS data for those obtained with commercially available standards according to Table 3. Quantification was performed with calibration curves ranging from 10 to 500 µg L −1 . The analyses were carried out in an EnSpire multi-detection microplate reader from PerkinElmer, using white polystyrene 96-well plates, purchased from Nunc (Roskilde, Denmark). The fluorescence was read from the top with an excitation wavelength of 485/20 nm and an emission filter of 528/20 nm.
The reaction was carried out in 75 mM sodium phosphate buffer (pH 7.4), in a 200 µL final volume. Fluorescein (FL, 40 nM, final concentration) and extract solutions in phosphate buffer (pH 7.4) were placed in each well of a 96-well plate. The mixtures were pre-incubated at 37 • C for 15 min, and 2,2'-azo-bis(2-amidinopropane)dihydrochloride (AAPH) solution (18 mM, final concentration) was added [35]. The microplate was immediately placed in the reader and automatically shaken prior to each reading. The fluorescence was recorded every 1 min for 80 min. A blank with FL and AAPH using buffer instead of the extract solution was used in each assay.
Five calibration solutions of Trolox (2-20 µM) as the antioxidant were also used in each assay. The inhibition capacity was expressed as concentration Trolox equivalents (TE) per 100 g of dry mass, and this was quantified by integration of the area under the fluorescence decay curve (AUC). All reaction mixtures were prepared in triplicate, and at least three independent assays were performed for each sample [36,52].

Evaluation of OH· Scavenging by ESR
The ESR (electron spin resonance) spectra were recorded in the X band (9.7 GHz) using a Bruker ECS 106 spectrometer with a rectangular cavity and 50-kHz field modulation, which was equipped with a high-sensitivity resonator at room temperature. The spectrometer conditions were: microwave frequency 9.81 GHz; microwave power 20 mW; modulation amplitude 0.91 G; receiver gain 59 db; time constant 81.92 ms; and conversion time 40.96 ms.
The scavenging activity of each derivative was estimated by comparing the DMPO-OH adduct signals in the antioxidant-radical reaction mixture and the control reaction at the identical reaction time. The scavenging activity is expressed as the scavenging percentage of hydroxyl radicals. The compound reactivity against hydroxyl radicals was investigated using the non-catalytic Fenton type method.
The samples were prepared as follows: 100 µL of the solvent used in extraction and 50 µL of NaOH (25 mM) were mixed. Then, 50 µL of DMPO (5,5-dimethyl-1-pyrroline-Noxide, 30 mM final concentration), 50 µL of the sample (20 mM in solvent used in extraction) and 50 µL of hydrogen peroxide (30%) were sequentially added. The mixture was put into an ESR cell, and the spectrum was recorded after five minutes of reaction [36,52,53].

DPPH Radical Scavenging Activity Evaluated by the ESR Method
The 1,1-diphenyl-2-picrylhydrazyl (DPPH·) radical scavenging capacities of the extracts were determined by electron spin resonance (ESR) spectrometry. All the reaction mixtures contained 1.0 mM of DPPH· (volume, 50 µL; final concentration, 250 mM) and 150 µL of each extract under study. Control experiments (without extract) were carried out. ESR signals were recorded after 3 min of reaction, as this was the time required to reach the reaction equilibrium. All the spectra were recorded in three scans in the same period. The scavenging activity of each compound was estimated by comparing the DPPH signals in the antioxidant-radical reaction mixture and the control reaction, at the same reaction time [40]. The results were expressed as the DPPH scavenging percentage. The extracts under study were added at final concentrations of 1 and 10 ppm. After 1 h of incubation, the medium was discarded, and the cells were washed twice gently with 200 µL of PBS. They were then incubated with AAPH at a final concentration of 600 mM (in PBS). The fluorescence was measured immediately after the addition of AAPH in a Perkin-Elmer EnSpire in 96-well plates at 37 • C using an excitation wavelength of 485 nm and an emission wavelength of 538 nm [36,40,48]. The evaluation was performed every minute for 1 h, and the CAA values were calculated using equation (x): CAA% = ∆F − ∆F AH ∆F × 100 F = fluorescence intensity in the presence of free radicals, without extract. F AH = fluorescence intensity in the presence of free radicals and extract. All measurements were made in the same period.

Conclusions
Argylia radiata is a polyextremophyle plant from the Atacama Desert that does not have normal seasonal growth. That is why tissue culture was used as a tool for high biomass production out of season. It could be used as a tool to investigate physiological changes that occur in plant cells (callus) and organs (aerial part) and, at the same time, as a source of value-add biotechnological compounds. In this work, extraction with polar solvents allowed for the isolation of polyphenolic and flavonoid compounds. Methanol appeared to be the best solvent to isolate polyphenolic compounds (60-67 mg GAE/100 g fresh tissue) from all tested tissue.
In contrast, water was the best solvent for isolating such compounds from natural plant roots (65 mg GAE/100 g fresh tissue). Both solvents showed a similar capacity for isolating flavonoids, and the callus tissue contained the lowest amount of such compounds. HPLC-MS/MS identified eight different secondary metabolites in the studied tissues. Caffeic acid, syringic acid and rutin were found in higher quantities. The chemical and biological antioxidant capacity establishes that the Argylia plant extracts show antioxidant properties against different radical species.
Moreover, the extract compounds can cross the plant cell wall barrier and displayed an antioxidant capacity in a dose-dependent manner inside cells. All these results suggest the potential application of vitroplant extracts as antioxidant compounds for different applications, i.e., food packaging, cosmetics, nutraceuticals, biopharma and other biotechnological uses. Finally, this work will open an opportunity for using extremophilic plants from the world's driest desert as a source of valuable compounds.