Comparative Investigation of Chemical Constituents of Kernels, Leaves, Husk, and Bark of Juglans regia L., Using HPLC-DAD-ESI-MS/MS Analysis and Evaluation of Their Antioxidant, Antidiabetic, and Anti-Inflammatory Activities

Leaves, husk, kernels, and bark methanolic extracts of Juglans regia L. were tested for their in vitro antidiabetic, anti-inflammatory, and antioxidant activities. For these purposes, α-amylase and α-glucosidase were used as the main enzymes to evaluate antidiabetic activities. Moreover, lipoxidase and tyrosinase activities were tested to estimate anti-inflammatory properties. Antioxidant properties of Juglans regia L., extracts were determined using three different assays. Leaves extract has an important radical scavenging activity and a-amylase inhibition. Similarly, husk extracts showed high total phenolic content (306.36 ± 4.74 mg gallic acid equivalent/g dry extract) with an important α-amylase inhibition (IC50 = 75.42 ± 0.99 µg/mL). Kernels exhibit significant tyrosinase (IC50 = 51.38 ± 0.81 µg/mL) correlated with antioxidant activities (p < 0.05). Husk and bark extracts also showed strong anti-lipoxidase activities with IC50 equal to 29.48 ± 0.28 and 28.58 ± 0.35 µg/mL, respectively. HPLC-DAD-ESI-MS/MS analysis highlights the phenolic profile of methanolic extracts of Juglans regia L. plant parts. The identified polyphenols were known for their antioxidant, antidiabetic (dicaffeoyl-quinic acid glycoside in kernels), and anti-inflammatory (3,4-dihydroxybenzoic acid in leaves) activities. Further investigations are needed to determine molecular mechanisms involved in these effects as well as to study the properties of the main identified compounds.


Introduction
Oxidative stress and inflammation have been considered important factors linked to diabetes mellitus, chronic pulmonary, cardiovascular, and Alzheimer's diseases, in addition to the physiopathology of cancer. The oxidative stress is known by the overproduction of ROS (reactive oxygen species) leading to the oxidation of macromolecules, notably lipids, proteins, and nucleic acids, which causes the impairment of cellular functions and The determination of total phenolic content in J. regia extracts was performed using the Folin-Ciocalteu procedure [38]. 0.5 mL of Folin-Ciocalteau reagent was added to 0.5 mL of each extract and agitated. Then, 4 mL of 7.5 % Na 2 CO 3 (w/v) was added, and the reaction was incubated at 45 • C for 30 min. Calibration curves were established using gallic acid. The absorbance was measured at 765 nm, and the total phenolic contents were expressed as mg gallic acid equivalents per g of the dry weight of extract (mg GAE/g of extract).

Total Flavonoid Content
The determination of the flavonoid content was carried out according to the aluminum trichloride method developed by Brighente et al. [39]. In test tubes, 1 mL of each of the extracts (1mg / mL) and 6.4 mL of distilled water were successively introduced, then 0.3 mL of the sodium nitrite solution (NaNO 2 5%) was added. After 5 min, 0.3 mL of aluminum trichloride (AlCl 3 10%) was added. After 6 min, 2 mL of sodium hydroxide (1 M) was added, and the solution was agitated and allowed to stand for 30 min. The absorbances were measured at 510 nm. Rutin was used as a standard under the same analytical conditions. The flavonoid content is expressed in mg of rutin equivalent per g of dried extract (mg RE/g of the extract).

Total Tannin Content
The determination of total tannin contents was effectuated using the Julkunen-Tiitto [40] method. 50 µL of each extract was mixed with 1.5 mL of 4% vanillin, then 750 µL of hydrochloric acid HCl was added. After, the mixture was incubated for 20 min at room temperature in the dark. Colchicine was used as standard. The absorbances were measured at 500 nm. The results are expressed in milligrams equivalent to catechin per gram of the extract (mg CE/g of extract).

HPLC-DAD-ESI-MS/MS
The chemical composition of J. regia methanolic extracts was determined by highperformance liquid chromatography (Hewlett-Packard 1100 Agilent Technologies) equipped with a DAD detector and an electrospray HP 1100 MSD API (Agilent-Technologies, Palo Alto, CA, USA) under analytical conditions reported by Pallaufa et al. [41]. A negative ionization mode, a capillary voltage of 3000 to 3500 V, and a fragmented variable of the order of 80 to 150 V were used. The column was a Poroshell 120 EC-C1, C18 (150 × 2.1) mm × 5 µm. The mobile phase was (A) 0.1% formic acid in the water, (B) acetonitrile. The established elution gradient was isocratic 15% B for 5 min, 15% B to 20% B over 5 min, 20-25% B over 10 min, 25-35% B over 10 min, 35-50% for 10 min. The total analysis time was 47 min, the flow rate was 0.5 mL/min. Double in-line detection was performed in the DAD using 280 nm and 370 nm as wavelengths and in a mass spectrometer (MS) connected to the HPLC system through the output of the DAD cell. MS detection was performed in a Qtrap API 3200 (Applied Biosystems, Darmstadt, Germany) equipped with an ESI source and a triple quadrupole ion trap mass analyzer. Zero-quality air was used as nebulizer gas (30 psi) and turbo gas for solvent drying (400 • C, 40 psi). Nitrogen served as a curtain (20 psi) and collision gas. The resolution of the quadrupoles was adjusted and the ion sputtering voltage was tuned at −4500 V (in negative mode). The MS detector has been set up to operate in two modes: Enhanced MS Analysis (EMS) and Enhanced Product Ion Analysis (EPIA) (EPI). To acquire an overview of all the ions in the sample, the EMS was utilized to capture the entire scan spectra. The defusing potential (DP) was −450 V, the input potential (EP) was −6 V, and the impact energy (CE) was −10 V. The spectra were recorded between m/z 100 and 1000 (in negative ion mode). For detected parent ion (s) discovered, the fragmentation pattern was then determined using EPI analysis under DP −50 V, EP −6 V, CE −25 V, and collision energy propagation (CES) 0 V [42].

DPPH Method
A concentration of 1 mg/mL of each extract was prepared and diluted in methanol to obtain a range concentration from 10 to 1000 µg/mL. 1 mL of each sample concentration or standard was mixed with 0.5 mL of 0.2 mM DPPH methanolic solution. Trolox was used as a standard under the same conditions. Absorbances were measured at 517 nm after 30 min of incubation at dark conditions [43]. The radical scavenging ability (RSA) was expressed in % according to the following equation where the Abs control is the absorbance of the solution containing all reagents except sample (or standard). IC50 was calculated from the plot of RSA vs. extract concentration.

ABTS Method
The antioxidant activity was determined by the 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method [44]. 2 mM of ABTS was mixed with 70 mM of potassium persulfate. After incubation in the dark during 12-16 h, the ABTS + solution was diluted with methanol to adjust absorbance to 0.700 ± 0.005 at 734 nm. Thus, 1 mL of each extract concentration (1 mg/mL) or standard was added to 2 mL of diluted ABTS solution to obtain a range concentration from 25 to 1000 µg/mL and incubated for 1 min, and the absorbance was measured at 734 nm. Trolox is used as a standard compound. Scavenging activity in this assay was expressed as the concentration of the extract required to inhibit 50% of the free radical scavenging activity.

FRAP Method
The extract's ferric ion-reducing power was determined by the FRAP method [45] with some modifications. A concentration of 1 mg/mL of each extract was prepared and diluted in methanol to obtain a range concentration from 5 to 50 µg/mL. Trolox was used as standard. 1 mL of each prepared extract or standard was mixed with 1.25 mL of 0.2 M sodium phosphate buffer (pH 6.6) and 1.25 mL of 1% potassium ferricyanide. The mixture was incubated at 50 • C for 20 min. After cooling, 1.25 mL of 10% trichloroacetic acid was added and centrifuged at 3000 rpm for 10 min. Finally, 1.25 mL of the supernatant was mixed with 1.25 mL distilled water and 0.25 mL FeCl 3 solution (0.1%, w/v). Absorbances were measured at 700 nm, and results were expressed as the 50% effective concentration (EC 50 ), which is the antioxidant concentration in mg/mL necessary to achieve an absorbance of 0.5.
2.6. Anti-Inflammatory Assay 2.6.1. Lipooxidase Inhibition Assay The lipooxidase inhibition assay was realized according to the procedure described by Debayo et al. [46]. A concentration of 1 mg/mL of each extract was prepared in methanol and diluted in 2M borate buffer to obtain a range concentration from 12.5 to 50 µg/mL. 12.5 µL of each extract concentration was added to 487.5 µL of 15-LOX (200 Units/mL) and kept at room temperature. After 5 min, 500 µL of linoleic acid dissolved in ethanol and diluted in the borate buffer were added to the enzymatic mixture and incubated for 5 min at room temperature. The absorbance was measured at 234 nm. Quercetin was used as a positive control, and DMSO was used as a negative control. The enzyme inhibition percentages were determined by the following equation. %Inhibition = (Abs extract − Abs blank) (Abs negative control − Abs blank) × 100%

Tyrosinase Inhibition Assay
The anti-tyrosinase activity was determined according to Huang et al. [47] procedure. A concentration of 1 mg/mL of each extract was prepared in methanol and diluted in 0.05 M PBS buffer (pH 6.5) to obtain a range concentration from 25 to 100 µg/mL. Tyrosinase enzymatic solution (333 U/mL) and L-Dopa (5 mM) were prepared in the PBS buffer. 50 µL of each extract concentration was mixed with 200 µL enzyme solution (3 U/mL), and the mixture was incubated at 37 • C. After 10 min, 500 µL of the substrate (L-Dopa) was added. The enzymatic reaction solution was then incubated for 30 min at 37 • C. The absorbance was measured at 510 nm. The percentage of inhibition was determined by the following formula, where Abs represents absorbance. Kojic acid was used as a standard.
The effect of J. regia extracts on α-amylase activity was assessed according to Kusano et al. [48] method with some modifications. A concentration of 1 mg/mL of each extract was prepared in methanol and diluted in phosphate buffer (pH 6.9) to obtain a range concentration from 25 to 100 µg/mL. 200 µL of starch solution (substrate) was added to 100 µL of the buffer, and 250 µL of α-amylase (30 U/mL) was then incubated at 37 • C for 15 min. For the sample test, the enzyme was incubated with 250 µL of each extract concentration for 15 min. After adding substrate, the enzymatic reaction was conducted for 15 min and then stopped using 400 µL HCl (0.1 M). Total and remaining starch were measured at 630 nm after adding 500 µL of iodine solution (25 mM). For the positive control, acarbose was used. The percentage of inhibition was calculated by the following formula.

α-Glucosidase Inhibition Assay
The α-Glucosidase inhibition activity was tested according to Li et al. described method with slight modifications [49]. A concentration of 1 mg/mL of each extract was prepared in methanol and diluted in 1 M potassium phosphate buffer (pH 6.8) to obtain a range concentration from 250 to 1000 µg/mL. 100 µL of sample or acarbose (positive control), 380 µL of p-nitro-phenyl-α-D-glucopyranoside (p-NPG) (0.53 mM), and 250 µL of α-Glucosidase solution (0.015 Units/mL) were mixed in the buffer. After incubating at 37 • C for 20 min, 1mL of Na 2 CO 3 (0.1 M) was added to quench the reaction. The IC 50 value is determined by the concentration of α-Glucosidase inhibitor necessary to inhibit 50% of activity under assay conditions. The absorbance was measured at 405 nm, and the inhibition percentages were determined using the following equation:

Statistical Analysis
Raw or log-transformed measured parameters were tested for normality and homogeneity of variance to meet the assumptions for parametric statistics. As the assumptions were violated for all parameters (extracts and assays), non-parametric analyses of variance, followed by a Dunn pairwise comparison test of means, were performed. The critical level of significance is set at 0.05.

Total Phenolic, Flavonoid, and Tocopherol Contents
The total phenolic content (TPC) analysis revealed a similar quantity of polyphenols in all methanolic extracts of J. regia, contrary to the total flavonoid content (TFC) analysis. The methanolic extract of bark and leaves showed the highest flavonoids quantity compared to husk and kernels, while the total tocopherol content (TTC) analysis showed the presence of tocopherols in low levels in bark methanolic extract (Table 1).  In MWB, nine compounds were detected (Table 2). Kaempferol diglucoside was detected at [M − H] − of m/z 447, as reported by Zeeshan et al. [50]. Three compounds were identified as Caffeic acid derivatives, among them caffeoyl hexoside (m/z 439) and caffeoyl hexose deoxyhexose (m/z 487), as reported by Appendino et al. [51].
In the MWH extract, nine compounds were detected (Table 4). Several chemical classes have been identified as flavonols, notably Quercetin pentoside with [M−H] − of m/z 435 at 37.25 minas reported by Gika et al. [54]. Caffeic acid derivatives were detected by three m/z peaks 339, 427, and 487 at 11.64, 27.11, and 36.38 min, respectively, as reported by Appendino et al. [51] and Ieri et al. [52]. Moreover, the flavones were presented by acacetin-aglycone (m/z 283) and apigenin-7-O-glucoside (m/z 431).  In the MWL extract, 11 compounds were identified (Table 5). Several molecules belong to flavonols, notably myricetin malonylglucoside (m/z 565) and kaempferol-malonyl glucoside (m/z 533), as reported by Vagiri et al. (2012). The two compounds with [M−H] − at m/z 437 and m/z 453 were identified as p-coumaroyl derivatives, as reported by Ieri et al. [52]. The carbohydrates were represented by Trigalloyl-glucose (m/z 633) and digalloyl-glucose (m/z 483), as reported by Gika et al. [54]. Thus, the caffeic acid derivatives were represented with [M−H] − at m/z 531 and m/z 487. J. regia extracts are rich in several bioactive components. The variation of phytochemical composition between J. regia parts may be due to the variation of patterns used in the synthesis, accumulation, and distribution of secondary metabolites, season, and environmental changes [55].
Contrary to FRAP assay results, the bark and the kernels methanolic extracts had the highest ABTS neutralization activity with IC 50 less than 88 µg/mL (Figure 1b), while Trolox showed an important antioxidant capacity (IC 50 = 15.2 µg/mL). In this study, the J. regia husk methanolic extract showed a pronounced ABTS neutralization activity (p < 0.05), which was higher than the results reported by Zhang et al. [30] with IC 50 of 324.8 µg/mL vs. 145.86 ± 1.61µg/mL. Similarly, Bhatia et al. [56] investigated the ABTS neutralization activity of the methanolic extract of J. regia bark. They found a low neutralizing activity compared to our results with an IC 50 of 601 µg/mL and 83.05 ± 0.39 µg/mL, respectively. On the other hand, the MWH extract had the strongest DPPH radical's scavenging capacity with IC 50 of 32.27 ± 0,69 µg/mL, which was significantly lower than that of leaves methanolic extract (p < 0.05) (Figure 1c). However, Trolox used as positive control inhibited DPPH radical at low concentrations (IC 50 = 2.04). Ghasemi et al. [31] investigated the antioxidant activity of MWH extract obtained from 11 different regions in Iran. IC 50 found in this previous study ranged from 122 ± 4.5 µg/mL to 302 ± 13 µg/mL, higher than IC 50 obtained in our study.
The methanolic extract of Tunisian J. regia bark showed a more pronounced radical scavenging activity (DPPH) compared to our results which were respectively IC 50 of 36 µg/mL vs. 123.42 ± 3.71 µg/mL [57] contrary to the aqueous extract which showed a low antioxidant capacity with an IC 50 of 582 µg/mL [56]. Concerning the methanolic extract of J. regia leaves, we obtained a similar result compared to the Jabli et al. study [58].

Anti-Lipoxidase Activity
The anti-inflammatory activity of the methanolic extracts of J. regia was performed in vitro by lipoxygenase (LOX) inhibition activity. All methanolic extracts of J. regia leaves, bark, husk, and kernels exhibited high lipoxygenase activity inhibition, respectively, with IC 50 of 28.38 ± 0.36, 28.51 ± 0.34, 29.48 ± 0.28, and 30.56 ± 0.36 µg/mL (Figure 2). These inhibitory activities are important compared to the standard (quercetin) (IC 50 = 6.72 µg/mL). Lipoxygenases are a group of enzymes implicated in the inflammation process regulation, the production of inflammatory mediators, notably leukotrienes, by catalyzing the oxidation of poly-unsaturated fatty acids, essentially arachidonic acid and linoleic acid, in addition to catalyzing the formation of eicosanoids from arachidonic acid and immune response regulation [59]. The over-expression of lipoxygenase contributes to several pathologies, such as Alzheimer's disease, diabetes, cancers, and cardiovascular disease, which further motivated research on natural compounds with anti-lipoxygenase activity [60].   [63].
This activity is strongly related to the phytochemical profile of the plants. Flavonoids, as well as other plant secondary metabolites, are well-known inhibitors of pro-inflammatory mediators, the first and most well-known being benzoic acid. In this study, its derivatives were present with relatively high doses in J. regia methanolic leaves extract. 3,4dihydroxybenzoic acid was investigated in numerous studies, and it was shown that it could directly bind the active site of soybean LOX, the latter sharing significant homologies with mammal's LOX [64]. Other derivatives of benzoic acid are of significant interest Multiple studies have been interested in the anti-LOX activity of other plants. We found that J. regia kernels demonstrated an excellent LOX inhibition activity compared with the methanolic extracts of Cydonia oblonga Mill fruit and Zanthoxylum armatum fruit with IC 50  This activity is strongly related to the phytochemical profile of the plants. Flavonoids, as well as other plant secondary metabolites, are well-known inhibitors of pro-inflammatory mediators, the first and most well-known being benzoic acid. In this study, its deriva-tives were present with relatively high doses in J. regia methanolic leaves extract. 3,4dihydroxybenzoic acid was investigated in numerous studies, and it was shown that it could directly bind the active site of soybean LOX, the latter sharing significant homologies with mammal's LOX [64]. Other derivatives of benzoic acid are of significant interest to human and animal nutrition [65]. Quercetin also demonstrated in vitro inhibitory effects on lipoxygenases, particularly 5-and 15-lipoxygenases [66]. Kaempferol also exhibited anti-lipoxygenase-1 activity but with a lesser inhibitory effect compared to quercetin [67].

Anti-Tyrosinase Activity
Tyrosinase is a metalloenzyme involved in the browning process of fruits and vegetables [68]. It also alters the melanogenesis process leading to chronic inflammation involved in several diseases. Melanin overproduction is associated with skin cancer and neurodegenerative disorders like Parkinson's disease. According to the physiopathological role of tyrosinase, several research studies were conducted on tyrosinase inhibitory effects of natural compounds [4,69,70].
J. regia methanolic extracts exhibited strong inhibition of tyrosinase activity, especially the methanolic extracts of kernels and bark, respectively, with IC 50 of 51.38 ± 0.82 µg/mL and 52.00 ± 0.56 µg/mL (p < 0.05) followed by the husk (IC 50 = 81.09 ± 0.40 µg/mL) and the leaves (IC 50 = 87.82 ± 0.87 µg/mL) (Figure 3). These results can be considered important since the used standard (kojic acid) showed an IC 50 value equal to 5.28 µg/mL.  [4,69,70]. J. regia methanolic extracts exhibited strong inhibition of tyrosinase activity, especially the methanolic extracts of kernels and bark, respectively, with IC50 of 51.38 ± 0.82 µg/mL and 52.00 ± 0.56 µg/mL (p < 0.05) followed by the husk (IC50= 81.09 ± 0.40 µg/mL) and the leaves (IC50= 87.82 ± 0.87 µg/mL) (Figure 3). These results can be considered important since the used standard (kojic acid) showed an IC50 value equal to 5.28 µg/mL. Previous studies on J. regia tyrosinase inhibitory activity reported that the water extract of J. regia leaves, seed, and husk have demonstrated an IC50 of 3.99 mg/mL, 8.83 mg/mL, and 10.13 mg/mL, respectively [71], the hydroethanolic extract of J. regia leaves presented an inhibition up to 50% at 751 ± 0.01 µg of extract/mL [72], in addition to a negligible antityrosinase activity in the extract of leaves collected in Turkey [73]. According to all these data, we note that the methanolic extract of J. regia collected from Morocco exhibits a significant anti-tyrosinase activity. Multiple studies have been focused on the anti-tyrosinase activity of other plant parts, notably the leaves of Ceratonia siliqua, the aerial parts of Cleome arabica and Pituranthos scoparius, whose exhibited moderate tyrosinase inhibition activity, respectively, with IC50 of 200 µg/mL, 124.4 ± 0.69 µg/mL and 125.01 ± 0.72 µg/mL. While other plant extracts had similar anti-tyrosinase activity to J. regia bark, such as Harpephyllum caffrum bark, contrariwise to the bark extracts of Hyaenanche globosa and Cassipourea flanaganii, which showed high activity against the tyrosinase with IC50 of 27.1 ± 042 µg/mL and 22.24 ± 1.32 µg/mL, respectively [74].
The methanolic extracts of kernels and bark phenolic profile showed mainly acacetin- Previous studies on J. regia tyrosinase inhibitory activity reported that the water extract of J. regia leaves, seed, and husk have demonstrated an IC 50 of 3.99 mg/mL, 8.83 mg/mL, and 10.13 mg/mL, respectively [71], the hydroethanolic extract of J. regia leaves presented an inhibition up to 50% at 751 ± 0.01 µg of extract/mL [72], in addition to a negligible antityrosinase activity in the extract of leaves collected in Turkey [73]. According to all these data, we note that the methanolic extract of J. regia collected from Morocco exhibits a significant anti-tyrosinase activity. Multiple studies have been focused on the antityrosinase activity of other plant parts, notably the leaves of Ceratonia siliqua, the aerial parts of Cleome arabica and Pituranthos scoparius, whose exhibited moderate tyrosinase inhibition activity, respectively, with IC 50 of 200 µg/mL, 124.4 ± 0.69 µg/mL and 125.01 ± 0.72 µg/mL. While other plant extracts had similar anti-tyrosinase activity to J. regia bark, such as Harpephyllum caffrum bark, contrariwise to the bark extracts of Hyaenanche globosa and Cassipourea flanaganii, which showed high activity against the tyrosinase with IC 50 of 27.1 ± 042 µg/mL and 22.24 ± 1.32 µg/mL, respectively [74].
The methanolic extracts of kernels and bark phenolic profile showed mainly acacetinaglycone and apigenin-7-O-glucoside. According to the literature, these flavones are encountered in many medicinal plants and are known for their therapeutic potential, including anti-inflammatory activities [75,76].
Our extracts showed the presence of myricetin, kaempferol, and quercetin which had been identified as tyrosinase inhibitors. Previous studies demonstrated that kaempferol and quercetin could inhibit the oxidation of L-DOPA catalyzed by tyrosinase [70]. Those molecules may be the actors behind the anti-tyrosinase activity of the methanolic extracts of J. regia parts.

Antidiabetic Activity
The antidiabetic activity of the different extracts of J. regia was assessed using the amylase and glycosidase inhibition assays. MWK and MWH highly inhibit the amylase activity with IC 50 values equal to 37.37 ± 1.16 µg/mL and 75.42 ± 0.99 µg/mL, respectively, which are significantly higher (p < 0.05) compared to MWL (IC 50 = 327.45 ± 3.13 µg/mL) (Figure 4a). Certainly, J. regia methanolic leaves extract (MWL) has the weakest inhibition effect on the α-amylase activity of all other extracts (p = 0.07), but it remained more effective compared with J. regia aqueous extract, which demonstrated an inhibition of 60% of amylase with 0.4 mg/mL [77]. These results are not significantly important compared with the inhibitory value of α-amylase shown by acarbose (26.11 µg/mL). Likewise, MWB extract released 50% of amylase activity at a concentration less than 210 µg/mL, and this remains more effective compared with bark aqueous extract of J. regia collected in Oued Amlil located in Taza region that showed an IC 50 of 5445.33 ± 82.58 µg/mL [78].
Quantitative analysis of J. regia extracts showed that the methanolic kernels extract has more polyphenols than flavonoids, and this may explain its effect on amylase because it has been shown that polyphenols bind with this digestive enzyme affecting starch hydrolysis activity in the small intestine reducing the absorption of glucose and consequently improving glycemic status in patients with diabetes [79,80]. This effect is mediated mainly by condensed and hydrolyzable tannins [79], and indeed, the qualitative analysis by HPLC-DAD-ESI-MS/MS demonstrated the dominance of gallotannin in this extract. In addition to that, the HPLC-DAD-ESI-MS/MS analysis showed that kernels of methanolic extract of J. regia contain a caffeoylquinic acid derivative, including dicaffeoyl-quinic acid glycoside. It was shown that this polyphenol class contributes as the most active principle against diabetes in vitro, and that may explain the excellent effect of this plant extract.
The evaluation of the inhibitory activity of J. regia extracts on α-glycosidase showed moderate results (compared to acarbose IC 50 = 0.35 µg/mL). MWL has the lowest IC 10 (266 ± 14.54 µg/mL) compared to MWB (922.03 ± 19.72 µg/mL), MWH (789.46 ± 7.19 µg/mL), MWK (978.92 ± 21,58 µg/mL) (Figure 4b). This effect may be due to caffeoylquinic acids, which can strongly inhibit glucosidase [81]. Previous studies had investigated the anti-glycosidase activity of other plants, notably the leaves of Annona senegalensis and Liquidambar formosana, which exhibited high glycosidase inhibition activity, respectively, with IC 50 of 97 µg/mL and 59 µg/mL compared to J. regia methanolic extracts [82]. J. regia leaf extracts have previously demonstrated an effective effect on diabetes in vitro and also in vivo by glycemia levels normalization via the inhibition of glucose-6-phosphate translocase and transporter GLUT2 in addition to the reduction of cholesterol synthesis by hydroxymethyl glutaryl-CoA reductase inhibition on rats thanks to caffeoylquinic acid and quercetin [35,37,83] and even in humans [84].
tive compared with J. regia aqueous extract, which demonstrated an inhibition of 60% of amylase with 0.4 mg/mL [77]. These results are not significantly important compared with the inhibitory value of α-amylase shown by acarbose (26.11 µg/mL). Likewise, MWB extract released 50% of amylase activity at a concentration less than 210 µg/mL, and this remains more effective compared with bark aqueous extract of J. regia collected in Oued Amlil located in Taza region that showed an IC50 of 5445.33 ± 82.58 µg/mL [78].

Correlations among J. regia Biochemical Activities in Different Extracts
To better understand the potential activities of different J. regia part extracts, we investigated the correlations among the biochemical activities, notably antioxidative activity, anti-inflammatory activity, and antidiabetic activity within each J. regia extract ( Figure 5). The analysis showed positive correlations between the anti-amylase activity and the antioxidant in the four extracts represented by the FRAP and DPPH assays. On the other hand, the anti-glucosidase activity of MWH and MWK was also positively correlated with the antioxidant activity, while this activity was correlated with TFC and TPC in MWL. As for the anti-inflammatory properties, the anti-LOX activity was correlated with the TFC and the antioxidant activity in MWB, MWH, and MWK, contrary to MWL, the anti-LOX activity was correlated with TPC and TFC. The anti-tyrosinase activity of MWK and MWL was correlated positively with TFC and FRAP, respectively. When we used a heatmap coupled with a dendrogram to highlight the relationships among different chemical screening within each extract, we found out that for the MWB extract (Figure 6), the chemical screenings are clustered into two distinct groups: (i) the first group contained FRAP, TPC, amylase et lipoxidase and (ii) the second group harbored tyrosinase, TFC, glycosidase, DPPH, and ATBS. Similarly, for the MWK (Figure 7) extract, the heatmap showed two groups: (i) the first one included TFC, ABTS, lipoxidase, tyrosinase, and glycosidase; (ii) the second one contained TPC, DPPH, FRAP, and amylase. As for the anti-inflammatory properties, the anti-LOX activity was correlated with the TFC and the antioxidant activity in MWB, MWH, and MWK, contrary to MWL, the anti-LOX activity was correlated with TPC and TFC. The anti-tyrosinase activity of MWK and MWL was correlated positively with TFC and FRAP, respectively. When we used a heatmap coupled with a dendrogram to highlight the relationships among different chemical screening within each extract, we found out that for the MWB extract (Figure 6), the chemical screenings are clustered into two distinct groups: (i) the first group contained FRAP, TPC, amylase et lipoxidase and (ii) the second group harbored tyrosinase, TFC, glycosidase, DPPH, and ATBS. Similarly, for the MWK (Figure 7) extract, the heatmap showed two groups: (i) the first one included TFC, ABTS, lipoxidase, tyrosinase, and glycosidase; (ii) the second one contained TPC, DPPH, FRAP, and amylase.   In the case of the MWH extract (Figure 8), (i) the first group is composed of ABTS, TPC, FRAP, and glycosidase, and (ii) the second group comprises amylase, lipoxidase, tyrosinase, TFC, and DPPH. For the MWL extract (Figure 9), there are two groups: (i) the first group encompasses ABTS, FRAP, amylase, tyrosinase, and DPPH, and (ii) the second group consists of glycosidase, lipoxidase, TPC, and TFC.   Definitively the chemical activity profiles varied deeply among the J. regia extracts; this finding should be taken into account when using the different extracts for therapeutic purposes.  Definitively the chemical activity profiles varied deeply among the J. regia extracts; this finding should be taken into account when using the different extracts for therapeutic purposes. Definitively the chemical activity profiles varied deeply among the J. regia extracts; this finding should be taken into account when using the different extracts for therapeutic purposes.

Conclusions
J. regia is a well-known species widely used for its nutritional benefits and also for its therapeutic properties, as reported in numerous ethnobotanical studies conducted in many countries.
This study is the first to emphasize the variability in antioxidant and biological characteristics across the various parts of the walnut. This variability will be used to guide in vivo studies and, consequently, the uses by herbalists of those parts of plants exhibiting the most relevant activities.
Results highlighted the potential antidiabetic properties of kernels and husk extracts as well as the anti-inflammatory properties of bark extract. In fact, the phenolic profile determined by HPLC-DAD-ESI-MS/MS showed the richness of J. regia in bioactive compounds. However, further investigations concerning the isolation of main chemical compounds, as well as the evaluation of their antioxidant, antidiabetic, and anti-inflammatory effects, are required to determine the molecular mechanisms involved in these biological activities. Moreover, in vivo explorations and toxicological investigations are needed to determine the main pharmaceutical parameters of these compounds as well as to validate their safety. Funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.