The Influence of Lactic Acid Fermentation on Selected Properties of Pickled Red, Yellow, and Green Bell Peppers

Red, yellow, and green peppers are vegetables rich in natural pigments. However, they belong to seasonal vegetables and need to be treated to prolong their shelf life. One new approach to processing vegetables is to pickle them using lactic acid bacteria. The use of such a process creates a new product with high health value, thanks to the active ingredients and lactic acid bacteria. Therefore, this study aimed to evaluate the effect of the applied strain of lactic acid bacteria (LAB) on the chemical properties, including the content of active compounds (pigments) and the physical properties of the peppers. Levilactobacillus brevis, Limosilactobacillus fermentum, and Lactoplantibacillus plantarum were used for fermentation and spontaneous fermentation. The pigments, polyphenols content, and antioxidant properties were determined in the pickled peppers, as well as sugar content, color, dry matter, texture properties, and the count of lactic acid bacteria. In all samples, similar growth of LAB was observed. Significant degradation of chlorophylls into pheophytins was observed after the fermentation process. No significant differences were observed in the parameters tested, depending on the addition of dedicated LAB strains. After the fermentation process, the vitamin C and total polyphenols content is what influenced the antioxidant activity of the samples. It can be stated that the fermentation process changed the red bell pepper samples in the smallest way and the green ones in the highest way.


Introduction
Fermentation is a process that has been used since ancient times. Once it was discovered and verified that fermented foods had no harmful effects on the human body, fermentation began to be used to preserve food [1]. Lactic fermentation is the enzymatic decomposition of organic substances into simple compounds under anaerobic conditions. This process is carried out by various types of lactic acid bacteria, converting monosaccharides and disaccharides into lactic acid and other compounds with the use of enzymes [2]. There are two types of lactic acid fermentation: spontaneous (fermentation using indigenous microflora) and controlled (fermentation with selected strains of lactic acid bacteria (LAB)) [3,4]. LAB play an important role in this process because they produce lactic acid, which is the main product in fermentation. LAB sugar metabolism is a common feature that connects it to the appropriate microorganisms, but it is also the fact that they are Grampositive and catalase-negative [1,5,6]. In a domestic setting, the most common fermentation mation on the physical and/or chemical properties of the silage obtained. The information available in the literature indicates a faster process using vegetable-dedicated strains of lactic acid bacteria. Fermentation of vegetables is the most common technique. In this type of lactic acid fermentation, the raw material is penetrated as a result of diffusion into the tissue of the salt, which comes from the brine, and the migration of water with mineral salts and sugars of low molecular weight into the brine [23]. This movement can change the structure and salt-sugar component within the vegetable. Furthermore, the acids, including lactic acid, produced during the fermentation process lower the pH in the brine, which may also involve changes in the pigments sensitive to low pH, such as chlorophylls.
Therefore, this study aimed to evaluate the effect of the fermentation process with different strains of lactic acid bacteria (LAB) on the chemical and physical properties of three colored bell peppers. Levilactobacillus brevis (LB), Limoslactobacillus fermentum (LF), and Lactoplantibacillus plantarum (LP) were used for fermentation; in addition, spontaneous fermentation with autochthonic bacteria (SF) was carried out. The changes in pigment content, antioxidant potential, and texture were evaluated.

Results and Discussion
Fermentation of bell peppers was conducted for seven days. In all bell pepper samples, the pH was at level 3.75-3.79, which confirmed that the fermentation process finished. This pH level is recommended for the LAB fermentation of vegetables [1].

Determination of Basic Properties of Fermented Bell Peppers
Three colored bell peppers were characterized by a low dry matter content. The highest dry matter content was seen for red bell peppers, then for yellow, and, lastly, for green. The same relation was observed for the sugar content, but greater differences were observed for the fructose values than the glucose values (Table 1). After the fermentation process, no statistically significant changes in the dry matter values were observed (Table 1), which could be linked to the sugar diffusion into the brine and, at the same time, NaCl diffusion into the tissue. Growth is related to sugar availability. After fermentation, a decrease in glucose and fructose was observed independently of the color of the bell peppers and the type of bacteria (Table 1). In all samples, saccharose was not determined. In red and green bell peppers, bacteria growth used all the glucose from the samples and almost all fructose content. After yellow bell pepper fermentation, the presence of glucose was observed, but not all sugars were used for bacteria growth. The exception was L. Brevis growth, for which the sugar content did not decrease at the same level as for other fermentation at the same time. However, in the fermentation conditions, growth for all LA bacteria strains was observed and did not differ from the others once. Other authors have stated that L. brevis prefers pentose sugar over glucose. The growth rates of L. brevis were found to retain maximum metabolic activity when grown with mixed sugars [24]. The absence of sucrose in the samples of fresh red and yellow bell peppers is not typical. Data for red bell peppers from USDA websites (0.11 g/g of fresh weight) and also from other researchers [25] showed that sucrose is present in red bell peppers. This discrepancy could be related to the type of bell peppers used for our research, which were bell peppers produced in Poland, not in Spain.

Color Coefficients
The color of the peppers was determined for each separately for the top and bottom. Peppers are a plant whose outer layer (top) is covered with a cuticle, which differs significantly in color from the tissue of the inner layer. The brightness (L*) of the top layer for yellow peppers was the highest, while the lowest values were observed for red and green peppers ( Table 1). The highest values of the redness coefficient (a*) were observed for red peppers, while the lowest values were observed for green peppers. In contrast, the values of the yellowness coefficient (b*) were highest for yellow peppers, while the values were similar for red and green peppers. Such relationships are related to the dominant main color in each of the analyzed peppers. After the fermentation process, a variation in the values of the color coefficients of the outer layer was observed, which was related to the color of the peppers and, also in some cases, to the type of strain. Only the brightness of the red peppers did not change after the fermentation process, whereas that of the yellow and green peppers did change when fermented with L. Plantarum and spontaneous fermentation. The redness values after fermentation for the red and yellow peppers decreased, while those for the green peppers increased. The post-fermentation coefficient for yellowness increased for red and green peppers, while no clear behavior was observed for yellow peppers once ( Table 1).
The color analysis of the bottom layer showed an increase in the values of redness and yellowness after the fermentation process for each of the analyzed peppers, regardless of the LA strain used. However, the brightness values after the fermentation process depended mainly on the color of the analyzed peppers; a decrease in the brightness values was observed for the red peppers, an increase for the yellow peppers, and no clear trend was observed for the green peppers (Table 1). These color changes can be related to the values observed after fermentation.

Salt Content
Analysis of SEM-EDS was made in all samples; however, in an article, only pictures of fresh and fermented L. plantarum were presented ( Figure 1). Identification for all samples is placed in the supplementary file in Figure S1. Results obtained from SEM-EDS analysis confirmed salt migration into the tissue ( Figure 1). All samples were cut in the middle and then placed in the SEM-EDS for analysis. The results ( Figure 1-information in tables, Figure S1 in supplementary file) showed that carbon, oxygen, potassium, and calcium were detected in fresh samples, but no chloride or sodium was observed. In the fermented samples, the detection of Na and Cl was confirmed.
The main difference in bell peppers was the detection of calcium and nitrogen in green fresh bell pepper, but not in other fresh bell peppers ( Figure 1e). From the fermented samples, nitrogen was detected only in green bell peppers fermented spontaneously ( Figure  S1c). In accordance with USDA data [26] on green bell peppers, a high amount of calcium was present in them in comparison to the red and yellow colors.
(e) (f) The main difference in bell peppers was the detection of calcium and nitrogen in green fresh bell pepper, but not in other fresh bell peppers (Figure 1e). From the fermented samples, nitrogen was detected only in green bell peppers fermented spontaneously (Figure S1c). In accordance with USDA data [26] on green bell peppers, a high amount of calcium was present in them in comparison to the red and yellow colors.

Bacteria Count
The pepper was inoculated with L. fermentum, L. brevis, and L. plantarum strains. Additionally, fermentation was carried out with the use of natural microflora found in pepper fruits. These heterofermentative strains (e.g., L brevis and L. fermentum) produce significant amounts of by-products, such as acetic acid and ethanol, which reduced yields [27]. Other groups of lactic acid bacteria known as facultatively heterofermentative, e.g., L. plantarum, use glucose via the Embden-Meyerhof pathway (EMP) to produce lactic acid; they may also have an inducible phosphoketolase (PK) pathway with pentose acting as an inducer. The third group, not tested here, is homofermentative lactic acid bacteria, including, e.g., Lactobacillus delbrueckii or L. lactis, which cannot convert xylose to lactic acid [28].
Fu and Mathews [29] found that lactic acid fermentation involving L. plantarum is homogeneous, and it is a homolactic bacterium associated with primary growth. L. brevis is a heterofermentative strain that has been shown to utilize xylose simultaneously with glucose, which is highly desirable as this strain has no repression of carbon catabolites.
The number of lactic bacteria increased over the course of fermentation ( Figure 2). An increase in the number of LABs by approximately two log cycles was observed during the 7-day fermentation. All types of peppers showed a similar increase in the number of bacteria. No significant influence of the lactic acid bacteria strain used for fermentation on the number of LAB during fermentation was observed.

Bacteria Count
The pepper was inoculated with L. fermentum, L. brevis, and L. plantarum strains. Additionally, fermentation was carried out with the use of natural microflora found in pepper fruits. These heterofermentative strains (e.g., L brevis and L. fermentum) produce significant amounts of by-products, such as acetic acid and ethanol, which reduced yields [27]. Other groups of lactic acid bacteria known as facultatively heterofermentative, e.g., L. plantarum, use glucose via the Embden-Meyerhof pathway (EMP) to produce lactic acid; they may also have an inducible phosphoketolase (PK) pathway with pentose acting as an inducer.
The third group, not tested here, is homofermentative lactic acid bacteria, including, e.g., Lactobacillus delbrueckii or L. lactis, which cannot convert xylose to lactic acid [28].
Fu and Mathews [29] found that lactic acid fermentation involving L. plantarum is homogeneous, and it is a homolactic bacterium associated with primary growth. L. brevis is a heterofermentative strain that has been shown to utilize xylose simultaneously with glucose, which is highly desirable as this strain has no repression of carbon catabolites.
The number of lactic bacteria increased over the course of fermentation ( Figure 2). An increase in the number of LABs by approximately two log cycles was observed during the 7-day fermentation. All types of peppers showed a similar increase in the number of bacteria. No significant influence of the lactic acid bacteria strain used for fermentation on the number of LAB during fermentation was observed.

Texture Analysis
Texture analysis (TPA) measures hardness, elasticity, cohesiveness, and chewiness. Hardness is defined as the force required to break down the sample in the mouth. Springiness is a measure of recovery during chewing; thus, it is a measure of the speed of recovery after the deforming force is removed. The third parameter of cohesiveness is related to the internal bonds that exist in the food; therefore, it informs about the force that keeps the sample cohesive. The last parameter, chewiness, represents the energy required to break down the solid food in order to ingest it [15,30,31]. In the presented research, different colors of fresh bell peppers were similar in all the TPA test results (Table 2). Similar results in springiness and chewiness were observed for fresh green and red bell peppers by Guiné and Barroca [30] and for fresh green peppers by Guiné and Barroca [15]. However, they observed higher results for cohesiveness, which could be related to the product itself or the size of the sample (in our tests, it was a cylindrical shape of sample approximately 5 mm high and with a diameter of 5 mm), while the water content is similar to that presented in our investigations. After the fermentation process, springiness values had significantly increased for all tested bell peppers, which could mean that a longer time of recovery is needed for the samples after fermentation.

Texture Analysis
Texture analysis (TPA) measures hardness, elasticity, cohesiveness, and chewiness. Hardness is defined as the force required to break down the sample in the mouth. Springiness is a measure of recovery during chewing; thus, it is a measure of the speed of recovery after the deforming force is removed. The third parameter of cohesiveness is related to the internal bonds that exist in the food; therefore, it informs about the force that keeps the sample cohesive. The last parameter, chewiness, represents the energy required to break down the solid food in order to ingest it [15,30,31]. In the presented research, different colors of fresh bell peppers were similar in all the TPA test results (Table 2). Similar results in springiness and chewiness were observed for fresh green and red bell peppers by Guiné and Barroca [30] and for fresh green peppers by Guiné and Barroca [15]. However, they observed higher results for cohesiveness, which could be related to the product itself or the size of the sample (in our tests, it was a cylindrical shape of sample approximately 5 mm high and with a diameter of 5 mm), while the water content is similar to that presented in our investigations. After the fermentation process, springiness values had significantly increased for all tested bell peppers, which could mean that a longer time of recovery is needed for the samples after fermentation.
For chewiness, an increase in values for yellow and green bell peppers was observed, while for red ones, no statistically significant differences were seen. That means no differences for red pepper will be seen with chewing the fermented bell pepper, while for yellow and green, where the value is almost 3-4 times higher than in fresh ones, this difference will be felt in the mouth. Cohesiveness for all bell peppers was the same as before the process. Only with green bell peppers did higher forces have to be used to bite the piece of pepper after fermentation. In comparison, texture properties for fermented kohlrabi [32] were much higher than in the presented study, which is linked to the structure of the kohlrabi, a root vegetable dissimilar to bell peppers. For three commercial varieties of Xiaomila (Capsicum frutescens L.), authors [33] observed that the hardness and chewiness of peppers significantly decreased after fermentation, which is different from our experiment. However, those two peppers differed in size, which can influence behavior during the fermentation process. However, the data for the hardness obtained for fresh Xiaomila pepper were at a similar level, while the chewiness was higher than in our research.

Pigment Amount and Identification
To date, research into the impact of the fermentation process involving LAB has mainly analyzed the effect of bacterial growth and sensory changes on raw materials. According to the data, the growth process of the lactic acid bacteria alone does not have a direct effect on changes or color degradation. However, the resulting metabolic products of bacteria, such as the production of lactic acid, resulted in an environment with a lower pH, which affects the pigments contained in the food. Furthermore, the vegetable fermentation process itself, i.e., flooding of the vegetable with brine of a specific concentration, creates concentration gradients in the system and can lead to an exchange of low-molecular-weight components from the tissue to the brine and from the brine to the raw tissue, which affects (and most of the time increases) the bioavailability of the pigments [34].
Data for pigment identification are placed in Table S1 in the supplementary file. Different colored bell peppers differ in pigment content (Table 3, Figure 3). In red bell pepper, the main part of the pigments is carotenoids-unbounded, monesters, and diesters. Main unbounded carotenoids were detected in increased values: capsorubin, zeaxanthin, lutein, capsanthin, β-cryptoxanthin, and β-carotene. Capsanthin palmitate and capsanthin dipalmitate were detected as the main mono and di-esters of the carotenoids ( Table 3). The same monocarotenoids and diesters were detected in yellow bell pepper (fresh and fermented), while in green there was no presence. In yellow and green bell peppers from unbounded carotenoids, only capsorubin, β-cryptoxanthin, and β-carotene were detected. In addition, pheophytin A was detected in yellow bell peppers, while pheophytin B and chlorophylls A and B were detected in green ones (Table 3). Only in green bell peppers did chlorophylls transform into pheophytin after fermentation, which is related to changes in the pH of the brine pH [6].    are olive-brown pigments and, as the main derivatives of chlorophyll, are formed by the loss of Mg 2+ from the porphyrin ring under low pH/acid conditions [35,36]. The transformation of chlorophyll to pheophytin changes the color of foods from green to olive-brown, which greatly affects the sensory quality. In addition, according to literature data, lower antioxidant and anti-inflammatory activity are observed after the transformation of chlorophyll to pheophytin [37]. Analysis of the pigment content divided into total carotenoids and pheophytins showed an increase after a fermentation process, while for chlorophylls decrease was observed ( Figure 3). These trends were also visible in each chemical compound presented in Table 3. The increase in total carotenoid content in all bell peppers after fermentation is related mostly to the disruption of tissues cells, inside which those carotenoids can be placed. A decrease in chlorophyll and an increase in pheophytins in green bell peppers, as mentioned above, is linked to the chlorophyll-pheophytins transformation in an acidic environment. Analysis of the influence of the starter culture on the pigment content showed that the highest increase in carotenoids was observed in all colors of bell peppers, and pheophytins in green peppers for fermentation with L. Plantarum as a starter culture. Hallmann, et al. [17] determined carotenoids content in fresh and fermented spontaneous method red bell peppers. Their investigation on fermented red bell peppers showed the presence of βcarotene, cis-βcarotene, α-carotene, capsorubin, cryptoxanthin, cryptoflavin, β-crypotxanthin, α-cryptoxanthin, lutein, zeaxanthin, and cis-ceaxanthin in both organic and conventional bell pepper types tested. Different levels are based on the cultivation, harvest time, and even production system. Analysis of the pigments content divided into the compound was made by Anaya-Esparza, et al. [13]. They compared different results for four colors of bell peppers. They presented the same compounds list as that obtained in our investigations.
It should be mentioned that chlorophyll is very sensitive and unstable when exposed to light, heat, oxygen, and pH. It can decompose to form pheophytin [35]. Pheophytins are olive-brown pigments and, as the main derivatives of chlorophyll, are formed by the loss of Mg 2+ from the porphyrin ring under low pH/acid conditions [35,36]. The transformation of chlorophyll to pheophytin changes the color of foods from green to olivebrown, which greatly affects the sensory quality. In addition, according to literature data, lower antioxidant and anti-inflammatory activity are observed after the transformation of chlorophyll to pheophytin [37].
Analysis of the pigment content divided into total carotenoids and pheophytins showed an increase after a fermentation process, while for chlorophylls decrease was observed (Figure 3). These trends were also visible in each chemical compound presented in Table 3. The increase in total carotenoid content in all bell peppers after fermentation is related mostly to the disruption of tissues cells, inside which those carotenoids can be placed. A decrease in chlorophyll and an increase in pheophytins in green bell peppers, as mentioned above, is linked to the chlorophyll-pheophytins transformation in an acidic environment. Analysis of the influence of the starter culture on the pigment content showed that the highest increase in carotenoids was observed in all colors of bell peppers, and pheophytins in green peppers for fermentation with L. Plantarum as a starter culture.

Antioxidant Properties
Bell peppers are characterized by their different colors and, therefore, different nutritional value. Due to its content of pigments and active compounds, such as phenols and flavonoids, bell peppers have a high antioxidant capacity [13,25,38]. This finding is confirmed by studies on the content (Figure 4a), according to which red bell peppers have the highest amount of phenolic compounds, expressed in mg of chlorogenic acid, followed by yellow and then green, the latter of which is much less. After the fermentation process, no clear trend related to the fluctuation of the phenolic compound was observed. Moreover, there was also no clear effect on their content in relation to the color of the pepper or the strain used. Hallmann et al. [17] tested red bell peppers in polyphenol content. They observed no changes in total phenolic compounds after the fermentation process in two types of red bell peppers.

Antioxidant Properties
Bell peppers are characterized by their different colors and, therefore, different nutritional value. Due to its content of pigments and active compounds, such as phenols and flavonoids, bell peppers have a high antioxidant capacity [13,25,38]. This finding is confirmed by studies on the content (Figure 4a), according to which red bell peppers have the highest amount of phenolic compounds, expressed in mg of chlorogenic acid, followed by yellow and then green, the latter of which is much less. After the fermentation process, no clear trend related to the fluctuation of the phenolic compound was observed. Moreover, there was also no clear effect on their content in relation to the color of the pepper or the strain used. Hallmann et al. [17] tested red bell peppers in polyphenol content. They observed no changes in total phenolic compounds after the fermentation process in two types of red bell peppers.   In addition to the flavonoid and pigment content, the antioxidant properties may also depend on the vitamin C content. Vitamin C content was tested in fresh and fermented samples. Similar to the phenolic compounds, the highest values were observed in red bell peppers, then in yellow, and the lowest was observed in green bell peppers (Figure 4b).
After the fermentation process is conducted independently of the bacteria strains, the vitamin C level rises. In green bell peppers, this increase did not depend on the fermentation bacteria strains, while for red and yellow, higher values were observed for fermentation with L. Plantarum and L. Fermentum. Zi, Zhixun, Meiqi, Xuetin, Hongbing, Xiaosong, and Junjie [33] reported the same increase in the vitamin C content in chili pepper after fermentation in comparison to the fresh pepper. However, in their research, the vitamin C in the red and green peppers was much higher than in ours. The authors stated that this level of vitamin C is related to longer sunlight when harvest is during summer.
The antioxidant values are presented in Figure 4c. They showed a similar correlation as was seen for vitamin C: the highest values are observed for red and yellow bell peppers fermented with L. plantarum.

Materials
Bell pepper (Capsicum annuum L.)-red (R), yellow (Y), and green (G)-were purchased from the Bronisze market (Warsaw, Poland) and stored in a temperature range of 4 to 6 °C. As an inoculum for fermentation, three strains were selected: Levilactobacillus brevis KKP 804 (LB), Limosilactobacillus fermentum KKP 811 (LF), and Lactoplantibacillus plantarum ATCC 4080 (LP). Spontaneous fermentation (SF) with autochthonic microbiota took place, as well. Reference strains were obtained from the Collection of Industrial Microorganisms (KKP, Warsaw, Poland) and American Type Culture Collection (ATCC, Manassas, VA, USA). Bacterial inoculum was prepared in physiological saline (0.85% NaCl) at a concentration of ~1 × 10 7 CFU/mL [39]. In addition to the flavonoid and pigment content, the antioxidant properties may also depend on the vitamin C content. Vitamin C content was tested in fresh and fermented samples. Similar to the phenolic compounds, the highest values were observed in red bell peppers, then in yellow, and the lowest was observed in green bell peppers (Figure 4b).
After the fermentation process is conducted independently of the bacteria strains, the vitamin C level rises. In green bell peppers, this increase did not depend on the fermentation bacteria strains, while for red and yellow, higher values were observed for fermentation with L. Plantarum and L. Fermentum. Zi, Zhixun, Meiqi, Xuetin, Hongbing, Xiaosong, and Junjie [33] reported the same increase in the vitamin C content in chili pepper after fermentation in comparison to the fresh pepper. However, in their research, the vitamin C in the red and green peppers was much higher than in ours. The authors stated that this level of vitamin C is related to longer sunlight when harvest is during summer.
The antioxidant values are presented in Figure 4c. They showed a similar correlation as was seen for vitamin C: the highest values are observed for red and yellow bell peppers fermented with L. plantarum.

Technological Treatment Fermentation Process
Fermentation was carried out in the cut vegetables. It was washed before pickling. In addition, the peppers that were to be inoculated with dedicated lactic acid bacteria were subjected to a 3 min soak in sodium hypochlorite to remove autochthonous microflora. Afterward, rectangles of about 2 × 3 cm were cut from the bell peppers. The cut vegetables (about 250 g) were placed into 500 mL jars, to which 230 mL of 2% v/v of NaCl solution was added. Then inoculum in the amount of 1% of the water volume was added. For spontaneous fermentation (SF), no inoculum was added. Jars were closed and kept in an incubator at a stable temperature of 28 • C. Fermentation was carried out for 7 days. All experiments were performed in duplicate.

Dry Matter Content
The dry matter was determined by the gravimetric method. Approximately 0.6-1 g of sample was placed in a dish and dried by the vacuum drying method (Memmert VO400, Schwabach, Germany) under the pressure of 10 mPa at 75 • C for 24 h until constant weight. Measurements were made in triplicate.

Color Parameters
Analysis of color was made in CR-5 (Konica Minolta Sensing Inc., Osaka, Japan) in a CIE L*a*b* system. Illuminant D65, angle 2 • , and calibration with white and black color. For all bell peppers, separate colors for top layer and bottom were taken. It is related to the huge difference in bell peppers. All measurements were made in 10 repetitions [40].

Texture Analysis
To determine the textural properties of raw and fermented vegetable samples, texture profile analysis were carried out. Texture profile analysis (TPA) of all the samples was performed using a Texture Analyzer TA.XT2i instrument (Stable Micro Systems, Surrey, UK) [15]. The cylindrical shape samples approximately 5 mm high and with a diameter of 5 mm were cut. Texture profile analysis was carried out by 2 compression cycles using a 75 mm diameter plunger, with a 5 s period of time between cycles. In addition, 5 kg force load cell and 0.5 mm/s test speed were used for the test. The textural properties of springiness, cohesiveness, and chewiness were calculated. Measurements were made in ten repetitions.

Vitamin C
The vitamin C content was determined using ultra-performance liquid chromatography (UPLC) according to Spínola et al. [41] with modification. The material was crushed in the analytical mill and extracted with a mixture of 3% MPA-8% acetic acid-1 mM EDTA. After centrifugation, the supernatant was filtered through 0.2 µm GHP ASCORDISC filters (Milipore, Burlington, MA, USA). An Acquity UPLC system (Waters Corporation, Milford, MA, USA) with a Waters Acquity UPLC photodiode array (PDA) detection system and EmpowerTM software (Waters Corporation, Milford, MA, USA) was used to record the detection signal and process the peak areas. The chromatograph is equipped with an Acquity HSS T3 analytical column (100 × 2.1 mm, 1.8 µm particle size). An isocratic mobile phase consisting of aqueous 0.1% (v/v) formic acid with a specific flow rate was used. The detection wavelength for the PDA was 245 nm. The L-ascorbic acid in the samples was determined by comparing it with the retention time of the standard and matching it with the UV absorption spectrum. Measurements were made in two repetitions.

Sugar Content
Sugar content was analyzed by HPLC (High Performance Liquid Chromatography) with refractive index detection (Waters, Milford, CT, USA) [42]. The material was homogenized in a laboratory mill and extracted with ultra-pure water (MilliQ redistilled water, 80 • C), then centrifugation for 5 min at 6000 rpm take place. The supernatant was filtered using a 0.22 µm GHP syringe filter and injected into the system. For separation, a Sugar-Pak I column with precolumn (Waters, Milford, CT, USA) was used. The temperature of the column thermostat was 90 • C and the detector temperature was 60 • C. Distilled water was used as the flow rate for the mobile phase with a flow rate of 0.6 mL/ min. The sugar content was determined on the basis of calibration curves obtained separately for each of the standards analyzed: sucrose, glucose, and fructose. Measurements were made in two repetitions.

SEM-EDS Analysis
Mineral composition was determined by scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) using an energy dispersion spectrometer (SDD type) integrated with a scanning electron microscope (Phenom-World B.V., FEI Company, Eindhoven, The Netherlands). Before analysis, the freeze-drying process was used (48 h, drying process temperatures 25 • C). The dried bell pepper samples were cut in half and placed on double-sticky tape. The analysis was carried out on at least four randomly selected points.

Pigment Content
The sample size of approximately 2 g was extracted using three portions of 10 mL of acetone/hexane 1:1 (v/v) containing butylated hydroxytoluene (0.5 g/L). The organic phases were collected and washed with sodium chloride solution (50 g/L) to remove water residues. Two milliliters were evaporated in vacuo and the residue was dissolved in isopropanol, membrane filtered (0.2 µm), and used for analysis.
The UPLC-HRMS analysis was conducted on a Vanquish LC system connected to a Q Exactive Focus Orbitrap mass spectrometer (both Thermo Fischer Scientific, Waltham, MA, USA) using their own modification of the method presented by Schweiggert et al. [43]. Samples were separated on a Cortex UPLC C18 column, 1. The mass spectrometer was fitted with an atmospheric pressure chemical ionization (APCI) source. The data acquisition and processing were performed using XCalibur software. The carotenoids and their esters were identified using the data from the original paper of Schweiggert et al. [43], while chlorophylls and pheophytins used the data of Huang et al. [44]. Positive mass spectra were recorded in the m/z range of 200-1400. The capillary temperature was set to 450 • C, while the spray voltage was 3 kV. Nitrogen was used as both sheath gas and auxiliary gas, with flow rates of 20.0 L/min and 5.0 L/min, respectively. The entire ion fragmentation approach (AIF) was used for the identification of analytes, while standard chlorophyll a, β-carotene, and zeaxanthin were used for their quantification. Measurements were made in two repetitions.

Total Phenolic Content and Antioxidant Activity
Antioxidant activity with Folin reagent was determined by using a spectrophotometric method. The extract was mixed with distilled water and a Folin-Ciocalteu solution, and after 3 min supersaturated sodium carbonate was added. After 1 h of incubation at 25 • C, the absorbance of the solution was measured at 750 nm [42]. A calibration curve standard for chlorogenic acid was established. Measurements were made in two repetitions.
In order to generate free radicals, a stock solution of DPPH was prepared within 24 h before the analysis [45]. In the beginning, in a 100 mL volumetric flask, a 25 mg of 2,2-diphenyl-1-picrylhydrazyl was weighed and made with a 99% methanol solution up to 100 mL. Working solutions of the radicals were prepared by diluting the stock solutions with 80% ethanol to obtain a concentration showing absorption in a 1 cm cuvette at a wavelength of 515 nm for DPPH at about 0.680-0.720 AU (absorbance unit). Reactions were performed in 96-well plates. At the same time, the absorbance of the radical working solutions was checked. Measurements were made in two repetitions. The antiradical activity was determined from the decrease in the absorbance of the radical solution in the presence of an antioxidant and expressed as mg Trolox Equivalent/g material.

Determination of the Number of Lactic Acid Bacteria
Total count by pour plate method was used to enumerate viable cells. Pepper samples were serially diluted using sterile saline (0.85% NaCl, Biomaxima, Poland). The samples were streaked onto plates de Man Rogosa and Sharpe Agar (MRS, Biomaxima, Poland) agar and incubated at 28 • C ± 1 • C for 48 h ± 4 h. The number of colonies grown was counted (ProtoCOL 3-Automatic colony counting and zone measuring, Synbiosis, USA) and recorded as log CFU per g. The samples were analyzed in triplicates. The analysis was performed after inoculation (in the case of samples with the addition of lactic acid bacteria) and on the day of starting spontaneous fermentation, and then on the 4th and 7th day of fermentation.

Statistical Treatment
The results obtained were subjected to a statistical analysis using Statistica 13 software (StatSoft, Warsaw, Poland). An analysis of multiple variances (ANOVA) was performed to assess the significance of the physical and chemical properties of the peppers. Results were divided into homogeneous groups using the Tukey HSD method (α = 0.05). Letters from homogeneous groups. The other parameters were determined using MS Excel 2019.

Conclusions
A similar increase was observed for the starter cultures in all samples tested. After the fermentation process, significant conversion of chlorophylls to pheophytins, as well as increased vitamin C and total polyphenols were observed, which affected the antioxidant activity of the samples. Texture properties showed no differences in hardness, while higher elasticity was observed, indicating that a long time would be needed for re-deformation of the fermented samples compared to the fresh ones. No differences in chewing values were observed for the red peppers, which may mean that the consumer will not experience differences between fresh and fermented peppers when biting and chewing; however, other (not tested, but observed) aroma and flavor characteristics will significantly differentiate these samples. It can be concluded that the fermentation process altered the red pepper samples the least and the green pepper samples the most.