Asperphenyltones A and B: New Phenylfuropyridinone Skeleton from an Endophytic Aspergillus sp. GXNU-A1

Chemical investigation of the fermentation extract of the mangrove endophytic fungus Aspergillus sp. GXNU-A1, isolated from Acanthus ilicifolius L., discovered an undescribed pair of enantiomers (asperphenyltones A and B (±1)), together with four previously described metabolites: nodulisporol (2), isosclerone (3), 2,3,4-trihydroxy-6-(hydroxymethyl)-5-methylbenzyl alcohol (4), and 4,6-dihydroxy-5-methoxy-7-methyl-1,3-dihydroisobenzofuran (5). Analyses of the 1D and 2D NMR spectroscopic data of the compounds supported their structural assignments. The presence of the asperphenyltones A and B, which are a pair of enantiomers, was established by HR-ESI-MS, 1D and 2D NMR data and confirmed by single-crystal X-ray diffraction analysis. Metabolites 1–5 were evaluated for their anti-inflammatory effects on the production of nitric oxide (NO), and 1, 3, and 4 showed significant potential inhibitory activities against NO production in activated macrophages with IC50 values of 26–40 μM, respectively.


Introduction
Marine fungi live in special marine environments, and their secondary metabolic pathways are very different from those of terrestrial fungi. Thus, metabolites of marine fungi are a promising source of active compounds [1,2]. Over the past ten years, mangrove endophytic fungi have been recognized as a potential source of pharmacologically important secondary metabolites [1,2]. Many secondary metabolites with unique structures and prominent biological activities from various marine-derived genera, Aspergillus and Penicillium, have been reported. [1]. For example, citridone A-C are rare derivatives of phenyl R-furopyridone, and have been reported from the cultured broth of Penicillium sp. FKI-1938 [3,4]. The phenyl R-furopyridone analogs, which represent a class of fungal metabolites with a core structure consisting of an R-furopyridone fused with a phenyl moiety, are an important source of structurally new and biologically active alkaloids [3][4][5][6][7]. These phenyl R-furopyridone analogs are produced from various fungal sources, such as Penicillium, Aspergillus, and Phomopsis [3][4][5][6][7]. Among them, phenyl R-furopyridone analogs from the marine-derived fungi of the genus have attracted considerable attention for their diverse structural complexity and promising bioactivities. For example, a series of phenyl R-furopyridone analogs (citridone A-C, from the Penicillium sp. FKI-1938) potentiates antifungal miconazole activity against Candida albicans [3,4], (±) citridone E shows cytotoxic activities against SF-268, MCF-7, HepG-2 and A549 cell lines with IC 50 values of 32.0, 29.5, 39.5 and 33.2 µM, respectively [7], and citridone I shows moderate inhibitory activity for nitric oxide production with an IC 50 value of 52.5 µM [6]. 2 of 7 We have reported the isolation of a series of new compounds from the mycelial extract of mangrove endophytic fungi isolated from Acanthus ilicifolius L., such as 2hydroacetoxydehydroaustin, asperlactone A, and guhypoxylonols A-D [8][9][10][11]. In our chemical investigation of this fungus, Aspergillus sp., GXNU-A1, a new phenyl R-furopyridone derivative 1, with four known metabolites 2-5, is discovered ( Figure 1). The structures of the isolated compounds are established by HR-ESI-MS and 1D and 2D NMR techniques, and this data is compared to the literature data. Herein, the isolation, structure determination, and anti-inflammatory activity of compounds 1-5 are described in detail.
Molecules 2022, 27, x FOR PEER REVIEW 2 of 7 potentiates antifungal miconazole activity against Candida albicans [3,4], (±) citridone E shows cytotoxic activities against SF-268, MCF-7, HepG-2 and A549 cell lines with IC50 values of 32.0, 29.5, 39.5 and 33.2 μM, respectively [7], and citridone I shows moderate inhibitory activity for nitric oxide production with an IC50 value of 52.5 μM [6]. We have reported the isolation of a series of new compounds from the mycelial extract of mangrove endophytic fungi isolated from Acanthus ilicifolius L., such as 2-hydroacetoxydehydroaustin, asperlactone A, and guhypoxylonols A-D [8][9][10][11]. In our chemical investigation of this fungus, Aspergillus sp., GXNU-A1, a new phenyl R-furopyridone derivative 1, with four known metabolites 2-5, is discovered ( Figure 1). The structures of the isolated compounds are established by HR-ESI-MS and 1D and 2D NMR techniques, and this data is compared to the literature data. Herein, the isolation, structure determination, and anti-inflammatory activity of compounds 1-5 are described in detail.
There is only one stereogenic carbon in 1, and its absolute configuration of C-5 was determined by experimental and calculated ECD ( Figure 3). However, the experimental ECD results showed an almost straight line without any absorption peaks, which indicates 1 should be a pair of enantiomers. The structure of 1 was further confirmed via singlecrystal X-ray diffraction ( Figure 4); its structure was assigned as a pair of enantiomers, and the double bond at C-9 and C-10 was identified as the Z-configuration. Unfortunately, they had not been isolated from each other due to only 1.3 mg of 1 being purified and tested. Therefore, the R and S configurations of 1 were named asperphenyltone A (+1) and asperphenyltone B (−1), respectively.  There is only one stereogenic carbon in 1, and its absolute configuration of C-5 was determined by experimental and calculated ECD ( Figure 3). However, the experimental ECD results showed an almost straight line without any absorption peaks, which indicates 1 should be a pair of enantiomers. The structure of 1 was further confirmed via singlecrystal X-ray diffraction ( Figure 4); its structure was assigned as a pair of enantiomers, and the double bond at C-9 and C-10 was identified as the Z-configuration. Unfortunately, they had not been isolated from each other due to only 1.3 mg of 1 being purified and tested. Therefore, the R and S configurations of 1 were named asperphenyltone A (+1) and asperphenyltone B (−1), respectively.  for NO production. Compound 2 had no anti-inflammatory properties under its safe concentration, which reveals that 1 may play a crucial role in anti-inflammatory activities.     Biogenetically, pyridone analogues were probably biosynthesized based on the compound (i) by cyclization and methyl migration of (i-1) ( Figure 5). The compound (i-2) was oxidated, and constricted the six-membered ring to a five-membered ring, conforming asperphenyltone A (+1) and asperphenyltone B (−1), further oxidation of asperphenyltone A (+1) converted it to citridone C [3][4][5][6][7]. The remaining known compounds 2-5 were determined by the analysis of their NMR (Figures S8-S15) data and comparing these with previously published data in the literature. They were identified as nodulisporol (2) [12], isosclerone (3) [13], 2,3,4-trihydroxy-6-(hydroxymethyl)-5-methylbenzyl alcohol (4) [14], and 4,6-dihydroxy-5-methoxy-7-methyl-1,3-dihydroisobenzofuran (5) [15].
The phenyl R-furopyridone analogs citridones H-L showed inhibitory activity for nitric oxide production (NO) [6]. The compounds (±1) have a similar structure to citridones, which may also possess inhibitory activities toward NO. Thus, compounds ±1-5 were evaluated for their anti-inflammatory effects on the production of nitric oxide (NO) in the RAW 264.7 macrophage cell line that was exposed to the inflammatory stimulus by lipopolysaccharide (LPS) ( Table 2). The results show that compound 1 has a potent inhibitory effect on NO release (IC 50 21 µM), while compounds 3-4 show weak inhibitory activities for NO production. Compound 2 had no anti-inflammatory properties under its safe concentration, which reveals that 1 may play a crucial role in anti-inflammatory activities. Biogenetically, pyridone analogues were probably biosynthesized based on the compound (i) by cyclization and methyl migration of (i-1) ( Figure 5). The compound (i-2) was oxidated, and constricted the six-membered ring to a five-membered ring, conforming asperphenyltone A (+1) and asperphenyltone B (−1), further oxidation of asperphenyltone A (+1) converted it to citridone C [3][4][5][6][7].

Fungal Material and Fermentation
The fungus was isolated from mangrove Acanthus ilicifolius L. leaves collected at the seaside of Qinzhou, Guangxi Province, China, in October 2019. The fungus strain GXNU-A1 was determined as an Aspergillus sp. by 18S rDNA analysis (GenBank accession number: MT626059). The strain was statically cultured at 28 °C for 30 days in 500 mL Erlenmeyer flasks (400 × 200 mL, a total of 80 L), each containing 200 mL of cultural media (glucose 20 g, yeast extract 2 g, MgSO4·7H2O 0.1 g, KH2PO4 0.2 g, sea salt 5 g in 1 L water).

Extraction and Isolation
The culture liquid (55 L) was filtered and extracted with EtOAc three times and then concentrated under vacuum to remove the EtOAc to obtain 8.6 g of metabolite extract. The mycelium was extracted by MeOH and then concentrated under vacuum. The concentrate was extracted with EtOAc and then combined with broth extract. The total extract (6.4 g) was fractionated by silica gel CC eluted on a gradient from dichloromethane (D)methanol (M) (D/M from 100:1 to 70:30) to yield six fractions (Frs.1-6, fraction 1 and 2 were obtained from D/M 10%, fraction 3 from 20%, fraction 3 and 4 from 30%, fraction 5 from 40% and fraction 6 from 50%), based on TLC analysis. Fr.3 was subjected to repeated CC and then purified by semi-preparative HPLC (10 ID × 250 mm, 4.0 mL/min, 60% MeOH in H2O) to afford compounds 1 (1.3 mg), 3 (colorless crystal, 2.5 mg) and 4

Fungal Material and Fermentation
The fungus was isolated from mangrove Acanthus ilicifolius L. leaves collected at the seaside of Qinzhou, Guangxi Province, China, in October 2019. The fungus strain GXNU-A1 was determined as an Aspergillus sp. by 18S rDNA analysis (GenBank accession number: MT626059). The strain was statically cultured at 28 • C for 30 days in 500 mL Erlenmeyer flasks (400 × 200 mL, a total of 80 L), each containing 200 mL of cultural media (glucose 20 g, yeast extract 2 g, MgSO 4 ·7H2O 0.1 g, KH2PO4 0.2 g, sea salt 5 g in 1 L water).

Extraction and Isolation
The culture liquid (55 L) was filtered and extracted with EtOAc three times and then concentrated under vacuum to remove the EtOAc to obtain 8.6 g of metabolite extract. The mycelium was extracted by MeOH and then concentrated under vacuum. The concentrate was extracted with EtOAc and then combined with broth extract. The total extract (6.4 g) was fractionated by silica gel CC eluted on a gradient from dichloromethane (D)-methanol (M) (D/M from 100:1 to 70:30) to yield six fractions (Frs.1-6, fraction 1 and 2 were obtained from D/M 10%, fraction 3 from 20%, fraction 3 and 4 from 30%, fraction 5 from 40% and fraction 6 from 50%), based on TLC analysis. Fr.3 was subjected to repeated CC and then purified by semi-preparative HPLC (

Anti-Inflammatory Assay
The anti-inflammatory effects of all compounds were examined for the production of nitric oxide (NO) in LPS-stimulated cells using a method according to our previously described method [16].

Conclusions
The chemical investigation of the mangrove endophytic fungus Aspergillus sp. GXNU-A1 isolated a new pair of phenyl R-furopyridone derivatives: asperphenyltone A and B, together with four known metabolites 2-5. Compounds 1-5 were evaluated for their anti-inflammatory effects on the production of NO, and compound 1 significantly reduced the production of NO in LPS-stimulated cells with an IC 50 value of 21 µM.