Tetrabromobisphenol Exposure Impairs Bovine Oocyte Maturation by Inducing Mitochondrial Dysfunction

Tetrabromobisphenol (TBBPA) is the most widely used brominated flame retardant in the world and displays toxicity to humans and animals. However, few studies have focused on its impact on oocyte maturation. Here, TBBPA was added to the culture medium of bovine cumulus–oocyte complexes (COCs) to examine its effect on oocytes. We found that TBBPA exposure displayed an adverse influence on oocyte maturation and subsequent embryonic development. The results of this study showed that TBBPA exposure induced oocyte meiotic failure by disturbing the polar-body extrusion of oocytes and the expansion of cumulus cells. We further found that TBBPA exposure led to defective spindle assembly and chromosome alignment. Meanwhile, TBBPA induced oxidative stress and early apoptosis by mediating the expression of superoxide dismutase 2 (SOD2). TBBPA exposure also caused mitochondrial dysfunction, displaying a decrease in mitochondrial membrane potential, mitochondrial content, mtDNA copy number, and ATP levels, which are regulated by the expression of pyruvate dehydrogenase kinase 3 (PDK3). In addition, the developmental competence of oocytes and the quality of blastocysts were also reduced after TBBPA treatment. These results demonstrated that TBBPA exposure impaired oocyte maturation and developmental competence by disrupting both nuclear and cytoplasmic maturation of the oocyte, which might have been caused by oxidative stress induced by mitochondrial dysfunction.


Introduction
Tetrabromobisphenol (TBBPA) is the most applied brominated flame retardant in the world, with production reaching over 2 million tons per year, which represents about 60% of global flame retardant production [1]. It is used in a range of paper, textile, electrical, and electronic appliances. It is detectable in ubiquitous environmental media, including air, water, soil, indoor dust, sediments, and sewage sludge. TBBPA can be bioaccumulated in animals and humans via diet, ingestion, and dermal contact with dust, which poses a threat to health [2]. Recent studies reported that TBBPA is present in many products, such as aquatic food, meat, egg, and especially milk [2,3]. Furthermore, TBBPA can be even detected in human maternal serum, breast milk, and umbilical cord serum [4].
Due to its extensive usage, more and more attention is paid to the toxicity of TBBPA. Several studies have found the potential hazards of TBBPA, including neurotoxicity, cytotoxicity, hepatotoxicity, and others. Therefore, TBBPA is listed in group 2A, "Probably carcinogenic to humans", by International Agency for Research on Cancer, which is based on sufficient evidence from animal experiments on carcinogenicity [5]. In addition, TBBPA

TBBPA Exposure Disrupts Bovine Oocyte Maturation and Cumulus-Cell Expansion
To detect the toxicity of TBBPA to bovine oocyte maturation, different concentrations of TBBPA (0, 20, 40, and 80 µM) were used in the culture medium. As shown in Figure 1A, TBBPA exposure impaired polar-body extrusion and cumulus-cell expansion. However, GVBD was not affected after TBBPT treatment ( Figure 1B). The quantitative analyses showed that all TBBPA treatment groups decreased the maturation rate to varying degrees compared with the control group, as reported in Figure 1C (ctrl, 87.78 ± 2.89%; 20 µM, 77.66 ± 2.71%; 40 µM, 65.82 ± 3.17%; 80 µM, 59.05 ± 3.13%). Because the 40 µM and 80 µM TBBPA treatments showed dramatic significance, 40 µM TBBPA was used in subsequent experiments. Cumulus-cell expansion was quantified using the CEI. TBBPA treatment decreased the mean CEI significantly ( Figure 1D). Confirming this, the mRNA expression level of expansion-related genes, TNFAIP6 and PTX3, also decreased significantly ( Figure 1E). The apoptosis of cumulus cells was also examined. As shown in Figure 1F,G, the apoptosis of cumulus cells increased after TBBPA treatment (p < 0.01). In addition, apoptosis was further confirmed by the ratio of BAX to BCL-2 ( Figure 1H). These results demonstrated that TBBPA exposure disrupted oocyte meiotic progression and cumulus-cell expansion.  (H) Ratio of BAX/BCL-2 in each group. * p < 0.05, ** p < 0.01, and *** p < 0.001, and ns p > 0.05.

TBBPA Exposure Destroys Spindle Morphology and Chromosome Alignment during Bovine Oocyte Maturation
Normal spindle morphology with aligned chromosomes is one of the most important indicators of high-quality oocytes. Therefore, oocyte spindle assembly and chromosome alignment were examined with immunofluorescent staining. As shown in Figure 2A, the oocytes displayed a typical barrel-shaped spindle with an aligned chromosome on the equatorial plate in the control group. By contrast, in the TBBPA-treated group, the proportion of abnormal spindle morphology with misaligned chromosomes was increased significantly ( Figure 2B,C). Oocyte maturation is a precision-coordinated process that is controlled by spindle assembly and chromosome alignment. An increased frequency of abnormal spindle and chromosome misalignment indicates that the microtubule stability and attachment between spindle and chromosome may be impaired after TBBPA exposure. These abnormalities can induce fertilization failure and subsequent embryonic development arrest. These results suggested that TBBPA exposure led to spindle defects and chromosome misalignment.

TBBPA Exposure Destroys Spindle Morphology and Chromosome Alignment during Bovine Oocyte Maturation
Normal spindle morphology with aligned chromosomes is one of the most important indicators of high-quality oocytes. Therefore, oocyte spindle assembly and chromosome alignment were examined with immunofluorescent staining. As shown in Figure 2A, the oocytes displayed a typical barrel-shaped spindle with an aligned chromosome on the equatorial plate in the control group. By contrast, in the TBBPA-treated group, the proportion of abnormal spindle morphology with misaligned chromosomes was increased significantly ( Figure 2B,C). Oocyte maturation is a precision-coordinated process that is controlled by spindle assembly and chromosome alignment. An increased frequency of abnormal spindle and chromosome misalignment indicates that the microtubule stability and attachment between spindle and chromosome may be impaired after TBBPA exposure. These abnormalities can induce fertilization failure and subsequent embryonic development arrest. These results suggested that TBBPA exposure led to spindle defects and chromosome misalignment.

TBBPA Exposure Causes Oxidative Stress to Induce Early Apoptosis
Previous studies have demonstrated that the toxicity of TBBPA is associated with oxidative stress [21][22][23]. We hypothesized that TBBPA exposure induced excessive ROS generation to result in oxidative stress. To confirm this hypothesis, ROS, GSH, and DHE levels were detected. The immunofluorescence results showed that ROS and DHE levels were significantly increased ( Figure 3A,E) and that the GSH level was decreased after TBBPA treatment ( Figure 3C). The measurement of fluorescence intensity was consistent

TBBPA Exposure Causes Oxidative Stress to Induce Early Apoptosis
Previous studies have demonstrated that the toxicity of TBBPA is associated with oxidative stress [21][22][23]. We hypothesized that TBBPA exposure induced excessive ROS generation to result in oxidative stress. To confirm this hypothesis, ROS, GSH, and DHE levels were detected. The immunofluorescence results showed that ROS and DHE levels were significantly increased ( Figure 3A,E) and that the GSH level was decreased after TBBPA treatment ( Figure 3C). The measurement of fluorescence intensity was consistent with this ( Figure 3B,D,F). To verify the effect of TBBPA on oxidative stress, superoxide dismutase 2 (SOD2) was analyzed. As a scavenger of mitochondrial superoxide, the SOD2 protein level was decreased significantly compared with the control group ( Figure 3G,H). These results showed that TBBPA induced oxidative stress by reducing ROS scavenging ability.
These results showed that TBBPA induced oxidative stress by reducing ROS scavenging ability.
Next, early apoptosis was detected after TBBPA treatment. As shown in Figure 3I, the annexin-v signal was barely detected in the control group. However, TBBPA-treated oocytes showed an obvious fluorescence signal. The rate of apoptosis was significantly increased in TBBPA-treated oocytes ( Figure 3J). These results indicated that TBBPA exposure impaired oocyte maturation because of oxidative-stress-induced apoptosis.  Next, early apoptosis was detected after TBBPA treatment. As shown in Figure 3I, the annexin-v signal was barely detected in the control group. However, TBBPA-treated oocytes showed an obvious fluorescence signal. The rate of apoptosis was significantly increased in TBBPA-treated oocytes ( Figure 3J). These results indicated that TBBPA exposure impaired oocyte maturation because of oxidative-stress-induced apoptosis.

TBBPA Exposure Induces the Mitochondrial Dysfunction
Mitochondria are the main organelles for ROS generation and are regarded to be among the most important markers of cytoplasmic maturation of oocytes. Therefore, mitochondrial function was examined. The mitochondria content of oocytes was first detected. The fluorescence results displayed that TBBPA exposure led to mitochondria signal reduc-tion ( Figure 4A). The quantitative analysis also demonstrated that the fluorescence intensity of mitochondria decreased dramatically ( Figure 4B). Furthermore, the mtDNA copy number also decreased significantly ( Figure 4C). The mitochondrial membrane potential (∆Ψm) is an essential indicator of mitochondrial function. ∆Ψm is a prerequisite for mitochondrial oxidative phosphorylation and the production of ATP. At the same time, it is conducive to maintaining the normal physiological functions of cells [24]. Oocytes from different groups were collected to evaluate mitochondrial function. ∆Ψm was detected using JC-1 staining in control and TBBPA groups. As shown in Figure 4D, JC-1 monomers displayed an obvious green signal, and JC-1 aggregates were the opposite. ∆Ψm revealed a sharp decline after TBBPA treatment ( Figure 4E). Altogether, these results suggested that TBBPA exposure impaired mitochondrial function.

TBBPA Exposure Induces the Mitochondrial Dysfunction
Mitochondria are the main organelles for ROS generation and are regarded to be among the most important markers of cytoplasmic maturation of oocytes. Therefore, mitochondrial function was examined. The mitochondria content of oocytes was first detected. The fluorescence results displayed that TBBPA exposure led to mitochondria signal reduction ( Figure 4A). The quantitative analysis also demonstrated that the fluorescence intensity of mitochondria decreased dramatically ( Figure 4B). Furthermore, the mtDNA copy number also decreased significantly ( Figure 4C). The mitochondrial membrane potential (ΔΨm) is an essential indicator of mitochondrial function. ΔΨm is a prerequisite for mitochondrial oxidative phosphorylation and the production of ATP. At the same time, it is conducive to maintaining the normal physiological functions of cells [24]. Oocytes from different groups were collected to evaluate mitochondrial function. ΔΨm was detected using JC-1 staining in control and TBBPA groups. As shown in Figure 4D, JC-1 monomers displayed an obvious green signal, and JC-1 aggregates were the opposite. ΔΨm revealed a sharp decline after TBBPA treatment ( Figure 4E). Altogether, these results suggested that TBBPA exposure impaired mitochondrial function.

TBBPA Exposure Causes Mitochondrial Dysfunction by Regulating PDK Activity
Mitochondria are the primary sites for ATP production, which is necessary to produce energy for cellular biological events. The ATP content was also examined after TBBPA treatment. As presented in Figure 5A, the ATP signal was much weaker in the TBBPA group than that in the control group. The fluorescence intensity was decreased significantly in TBBPA-treated oocytes ( Figure 5C). Pyruvate dehydrogenase kinase

TBBPA Exposure Causes Mitochondrial Dysfunction by Regulating PDK Activity
Mitochondria are the primary sites for ATP production, which is necessary to produce energy for cellular biological events. The ATP content was also examined after TBBPA treatment. As presented in Figure 5A, the ATP signal was much weaker in the TBBPA group than that in the control group. The fluorescence intensity was decreased significantly in TBBPA-treated oocytes ( Figure 5C). Pyruvate dehydrogenase kinase (PDK) is a key factor to participate in pyruvate metabolism. We speculated that TBBPA disrupted ATP supplements by regulating the PDK pathway. To confirm this, PDK3 was determined after TBBPA exposure. We found that PDK3 fluorescence intensity increased significantly ( Figure 5B,D). These results indicated that TBBPA impaired oocyte maturation because of PDK3-induced ATP deficiency.
(PDK) is a key factor to participate in pyruvate metabolism. We speculated that TBBPA disrupted ATP supplements by regulating the PDK pathway. To confirm this, PDK3 was determined after TBBPA exposure. We found that PDK3 fluorescence intensity increased significantly ( Figure 5B,D). These results indicated that TBBPA impaired oocyte maturation because of PDK3-induced ATP deficiency.

TBBPA Exposure Reduces Oocyte Competence
To test oocyte competence, oocytes were fertilized and cultured until the blastocyst stage. We found that the majority of oocytes could be fertilized and developed to the twocell stage in the control group (76.63%), while fewer TBBPA-treated oocytes could develop to the two-cell stage (66.24%). The fertilization ability of TBBPA-exposed oocytes decreased significantly. The embryos were cultured until the blastocyst stage. The results showed that the four-cell rate (71.68% vs. 52.83%), eight-cell rate (62.38% vs. 47.44%), morula stage (53.89% vs. 42.04%), and blastocyst rate (32.37% vs. 15.06%) were also dramatically reduced in the TBBPA-treated-oocyte group ( Figure 6A,B). In addition, we also found that the apoptotic rate of blastocysts also decreased significantly after TBBPA

TBBPA Exposure Reduces Oocyte Competence
To test oocyte competence, oocytes were fertilized and cultured until the blastocyst stage. We found that the majority of oocytes could be fertilized and developed to the twocell stage in the control group (76.63%), while fewer TBBPA-treated oocytes could develop to the two-cell stage (66.24%). The fertilization ability of TBBPA-exposed oocytes decreased significantly. The embryos were cultured until the blastocyst stage. The results showed that the four-cell rate (71.68% vs. 52.83%), eight-cell rate (62.38% vs. 47.44%), morula stage (53.89% vs. 42.04%), and blastocyst rate (32.37% vs. 15.06%) were also dramatically reduced in the TBBPA-treated-oocyte group ( Figure 6A,B). In addition, we also found that the apoptotic rate of blastocysts also decreased significantly after TBBPA treatment ( Figure 6C,D). These results displayed that TBBPA exposure induced the poor quality of oocytes, which resulted in the decreased competence of IVF embryos.  Figure 6C,D). These results displayed that TBBPA exposure induced the poor quality of oocytes, which resulted in the decreased competence of IVF embryos.

Discussion
Oocyte maturation is a complicated progression, and changes in the internal and external environment give rise to fertilization failure, embryonic development arrest, and even abortion. Increasing evidence demonstrates that environmental and industrial pollution can disrupt oocyte maturation and even cause infertility [25][26][27]. As the most widely used brominated flame retardant, TBBPA has confirmed toxicity, including neurotoxicity, immunotoxicity, cytotoxicity, etc. However, the influences of TBBPA on female germ cells are unknown. The oocyte is the most important cell in female reproduction. Therefore, the effect of TBBPA on oocytes was examined in this study.
For this purpose, oocyte maturation indicators and related signaling pathways were examined after COCs were treated with TBBPA. Our results showed that TBBPA treatment impaired oocyte meiotic progression and the expansion of cumulus cells. Meanwhile, cumulus-cell apoptosis was also induced after TBBPA exposure. The bidirectional communication between the oocyte and cumulus was crucial to producing a healthy oocyte.

Discussion
Oocyte maturation is a complicated progression, and changes in the internal and external environment give rise to fertilization failure, embryonic development arrest, and even abortion. Increasing evidence demonstrates that environmental and industrial pollution can disrupt oocyte maturation and even cause infertility [25][26][27]. As the most widely used brominated flame retardant, TBBPA has confirmed toxicity, including neurotoxicity, immunotoxicity, cytotoxicity, etc. However, the influences of TBBPA on female germ cells are unknown. The oocyte is the most important cell in female reproduction. Therefore, the effect of TBBPA on oocytes was examined in this study.
For this purpose, oocyte maturation indicators and related signaling pathways were examined after COCs were treated with TBBPA. Our results showed that TBBPA treatment impaired oocyte meiotic progression and the expansion of cumulus cells. Meanwhile, cumulus-cell apoptosis was also induced after TBBPA exposure. The bidirectional communication between the oocyte and cumulus was crucial to producing a healthy oocyte. Cumulus-cell expansion is necessary for oocyte maturation and subsequent embryonic development. Cumulus cells supply substrates for energy metabolism and biosynthesis to the oocyte [28,29]. Previous studies have also showed that cumulus-cell apoptosis is regarded as a marker for oocyte competence [30]. Therefore, TBBPA displays reproductive toxicity not only to oocyte maturation but also to cumulus cells, which in turn regulate oocyte maturation.
Oocyte maturation contains nuclear and cytoplasm maturation. The effect of TBBPA on the nuclear maturation of oocytes was confirmed with experiments that showed an increased rate of disorganized spindle assembly and misaligned chromosomes. The accurate control of spindle assembly and chromosome alignment are indispensable to producing a good-quality oocyte [31,32]. The abnormality of spindle assembly and chromosome alignment causes aneuploidy and subsequent embryonic development arrest [33]. The ROS level is a key indicator of cytoplasm maturation [34,35]. The excessive accumulation of ROS induces oxidative stress. As expected, TBBPA treatment significantly elevated ROS and DHE levels and decreased GSH levels. GSH is regarded as an antioxidant that eliminates ROS [36]. In addition, SOD2, which is the main scavenger of ROS, was significantly decreased. TBBPA may reduce the ability to clear ROS by regulating SOD2. ROS accumulation caused the occurrence of early apoptosis, which was confirmed with Annexin-V staining. TBBPA caused the excessive accumulation of ROS by decreasing the ability to scavenge ROS, further inducing early apoptosis. These results present that TBBPA exposure reduced oocyte quality by impairing both nuclear and cytoplasmic maturation of oocytes.
Mitochondria are the main organelles for ROS production and the main targets of ROS. Mitochondria also play an important role in programmed cell death, and their morphology is critical for apoptosis [37]. Therefore, mitochondria are essential for ATP synthesis and redox maintenance [38,39]. In addition, mitochondria also supply ATP to organize spindles and arrange chromosomes during the oocyte meiotic process [40]. Mitochondrial dysfunction can lead to oocyte maturation failure [41]. Therefore, the function of mitochondria is essential for oocyte maturation. The function of mitochondria was evaluated by examining the content of mitochondria, mtDNA copy number, and mitochondrial membrane potential. We found that TBBPA exposure resulted in mitochondrial dysfunction. These results suggested that TBBPA impaired mitochondria function, inducing oxidative stress.
The mechanism of TBBPA and its effect on oocyte maturation is to be further studied. Mitochondria are energy factories that ensure biological processes. It is known that oocytes cannot utilize glucose directly, which needs to be transformed into pyruvate by cumulus cells. Pyruvate is the main energy source throughout oocyte maturation [42]. Pyruvate is converted to acetyl coenzyme A by the PDK-mediated phosphorylation of pyruvate dehydrogenase (PDH) and enters into the tricarboxylic acid cycle to produce ATP. PDK3 shows higher affinity with the pyruvate dehydrogenase complex than other PDKs [43]. In addition, PDK3 mRNA is most abundant than others in mouse oocytes. A previous study illustrated that PDK3, which is considered to be the primary PDK, regulates metabolism via the phosphorylation of PDH. This study also showed that the overexpression of PDK3 not only causes metabolic dysfunction but also induces spindle assembly defects and chromosome misalignment [43]. Therefore, PDK3 displays an effect on spindle organization besides regulation metabolism. Accordingly, our results showed that TBBPA exposure decreased the ATP contents and increased the PDK3 level. These results illustrated that TBBPA exposure destroyed pyruvate usage via the PDK3 axis to disturb ATP generation. In addition, TBBPA-induced meiotic defects may be associated with PDK3. However, the mechanism needs to be further studied.

Materials and Methods
All the chemicals and reagents used for this study were purchased from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated.

TBBPA Treatment
The COCs were randomly divided into the following four groups: control, 20 µM TBBPA, 40 µM TBBPA, and 80 µM TBBPA. The control group was cultured in IVM medium, while the other groups were treated with TBBPA (80 mM in DMSO), which was diluted to 20 µM, 40 µM, and 80 µM with IVM medium. The final concentration of DMSO was 0.1%.

Evaluation of Cumulus Expansion
After 22 h of the culturing of COCs, the cumulus expansion index (CEI) was calculated according to a previous study [44]. Briefly, cumulus expansion could be divided into five levels: grade 0, no cumulus expansion, oocytes attached to the bottom of the dish; grade 1, only the outermost 1-2 cumulus granulosa cells expanded; grade 2, the outer cumulus granulosa cells expanded radially, and the whole COCs were observed to be fluffy; grade 3, the radial crown part did not expand,

Measurement of mtDNA Copy Number
The measurement of the mtDNA copy number was based on a previous study [45]. To extract DNA, 10 denude oocytes were collected in 2 µL of PBS. Oocytes were centrifuged at 12,000× g for 30 s at 4 • C. A volume of 8 µL of 5 mM Tris-HCl was added to each sample, and all samples were heated to 95 • C for 10 min. Proteinase K was added to the samples. Samples were heated at 55 • C for 30 min followed by heating at 95 • C for 10 min for proteinase K deactivation. The lysate was used for the measurement of the mtDNA copy number. The mtDNA copy number was measured using SYBR Green PCR Master Mix and a Rotor-GeneTM 6000 real-time rotary analyzer.

Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
Total mRNA was extracted using Arcturus ® PicoPure ® RNA Isolation Kit (Thermo, KIT0204). For the specific methods, please refer to the kit manual. The concentration and purity of the extracted RNA were quantified using a NanoDrop 2000C spectrophotometer, and a PrimeScriptTM RT kit was used to synthesize cDNA. All the primers used in this experiment were synthesized by Sangon Bioengineering Co., Ltd. (Shanghai, China), and their sequences are shown in Table 1. The 18S RNA gene was used as a reference gene, and the standard curves of all the primers were verified. RT-PCR was performed using SYBR Green PCR Master Mix and a Rotor-GeneTM 6000 real-time rotary analyzer.

IVF and Embryo Culture
After the control and TBBPA-treated groups were cultured for 22 h, the matured oocytes were washed and cultured in fertilization medium (50 µL), overlaid with mineral oil, and incubated in a humidified atmosphere of 5% CO 2 at 38.5 • C. Frozen bull semen straws were thawed by immersing them in a water bath at 37.5 • C for 12 sec. Sperm was then gradient-centrifuged using 90% percoll solution 700 rcf for 15 min. The 90% percoll solution comprised 46.75 mg/mL NaCl, 2.3 mg/mL KCl, 0.35 mg/mL NaH 2 PO 4 , 23.8 mg/mL HEPES, and 2.09 mg/mL NaHCO 3 . Subsequently, sperm was centrifuged with TALP medium at 300 rcf for 5 min. The TALP medium comprised 5.84 mg/mL NaCl, 0.23 mg/mL KCl, 0.31 mg/mL CaCl 2 , 0.035 mg/mL NaH 2 PO 4 , 0.08 mg/mL MgCl 2 , and 2.1 mg/mL NaHCO 3 . The matured oocytes were co-incubated with spermatozoa in the fertilization medium for 16 h in a humidified atmosphere of 5% CO 2 at 38.5 • C. Twenty hours post-IVF, remaining cumulus cells were removed by vortexing, and presumptive zygotes were cultured in synthetic oviduct fluid (SOF) supplemented with 5% FBS until blastocysts hatched from the zona pellucida (days 8-10). In vitro culture (IVC) was carried out under mineral oil at 38.5 • C in an atmosphere of 5% CO 2 and 5% O 2 in air with maximum humidity.

Statistical Analyses
The data obtained from three replicates were reported as means ± standard errors (SEMs). Statistical analyses were performed with the one-way analysis of variance (ANOVA) and the independent-sample t-test using SPSS software, version 26.0 (SPSS, Chicago, IL, USA). Figures were generated using the GraphPad Prism software package (version 6.01; GraphPad, La Jolla, CA, USA). The average immunofluorescence intensity was measured and analyzed with Image J software. RT-PCR results were analyzed with the 2 −∆∆ Ct method. p < 0.05 was considered to be statistically significant.

Conclusions
In summary, we discovered that TBBPA exposure destroyed bovine oocyte maturation because of the abnormity of nuclear and cytoplasmic maturation. The study showed that TBBPA exposure induced mitochondrial dysfunction, which resulted in oxidative stress and early apoptosis. In addition, mitochondrial dysfunction led to insufficient energy supply to disrupt spindle assembly and chromosome alignment via regulating pyruvate metabolism mediated by PDK3. Therefore, this study indicated that the toxicity of TBBPA to oocytes gave rise to mitochondrial dysfunction inducing oxidative stress and early apoptosis by regulating PDK3.

Data Availability Statement:
The data presented in this study are available upon request from the corresponding author.

Conflicts of Interest:
The authors declare no conflict of interest.