Limoniastrum monopetalum–Mediated Nanoparticles and Biomedicines: In Silico Study and Molecular Prediction of Biomolecules

An in silico approach applying computer-simulated models helps enhance biomedicines by sightseeing the pharmacology of potential therapeutics. Currently, an in silico study combined with in vitro assays investigated the antimicrobial ability of Limoniastrum monopetalum and silver nanoparticles (AgNPs) fabricated by its aid. AgNPs mediated by L. monopetalum were characterized using FTIR, TEM, SEM, and DLS. L. monopetalum metabolites were detected by QTOF–LCMS and assessed using an in silico study for pharmacological properties. The antibacterial ability of an L. monopetalum extract and AgNPs was investigated. PASS Online predictions and the swissADME web server were used for antibacterial activity and potential molecular target metabolites, respectively. Spherical AgNPs with a 68.79 nm average size diameter were obtained. Twelve biomolecules (ferulic acid, trihydroxy-octadecenoic acid, catechin, pinoresinol, gallic acid, myricetin, 6-hydroxyluteolin, 6,7-dihydroxy-5-methoxy 7-O-β-d-glucopyranoside, methyl gallate, isorhamnetin, chlorogenic acid, 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxo-4H-chromen-3-yl 6-O-(6-deoxy-β-l-mannopyranosyl)-β-d-glucopyranoside) were identified. The L. monopetalum extract and AgNPs displayed antibacterial effects. The computational study suggested that L. Monopetalum metabolites could hold promising antibacterial activity with minimal toxicity and an acceptable pharmaceutical profile. The in silico approach indicated that metabolites 8 and 12 have the highest antibacterial activity, and swissADME web server results suggested the CA II enzyme as a potential molecular target for both metabolites. Novel therapeutic agents could be discovered using in silico molecular target prediction combined with in vitro studies. Among L. Monopetalum metabolites, metabolite 12 could serve as a starting point for potential antibacterial treatment for several human bacterial infections.


Introduction
Antibiotic is the gold standard of infectious disease treatment, though misuse may lead to a variety of undesirable consequences, such as the development of a wide range of resistant bacteria [1]. In relation to the World Health Organization (WHO), MDR is a serious public health risk, as several pathogenic bacteria have evolved as non-sensitive to numerous antibacterial agents [2]. The WHO recently confirmed that new sources of antibiotics are urgently required to reduce the global spread of antibiotic resistance. Different promising approaches have been developed to minimize the wide spread of MDR. Nanotechnology is considering a stepping stone for controlling and preventing the development of MDR. Nanoparticles (NPs) have a variety of biomedical applications, such as antibacterial, antifungal, and antiviral agents. In contrast to bulk materials, NPs have distinct properties, including shape, size, distribution, and surface area, which facilitates their attachment to ligands. The potential of NPs relies mainly on the size of the practical, which improves their ability in bacterial cell penetration, which leads to cell destruction via various mechanisms. Therefore, nano-antibiotics might be considered an excellent substitute to present antimicrobial therapy in combating MDR bacteria. Importantly, they have been approved by the Food and Drug Administration (FDA), and about 1000 commercial nanoproducts are currently available in the market worldwide [3,4]. Besides their well-known effectiveness in the biomedical field, NPs have been widely used in the past few years in various applications, such as electronic, catalysis, and optical applications. Silver nanoparticles (AgNPs) have been widely used due to their high antimicrobial property, which has led to the improvement of a wide variety of silver nanoproducts, such as nano-silver-coated wound dressings [5]. Kovács et al. [6] also evaluated the aptitude of AgNPs for cancer mitigation through different attitudes, such as interaction with cell components, development in tumor tissues besides oxidative stress, and activation of numerous signaling pathways. AgNP syntheses could be established using different techniques, including chemicals and physicals. Producing AgNPs by physicochemical routes requires expensive equipment and long reaction times and releases toxic byproducts that are hazardous to the environment. To conquer these disadvantages, a green synthesis of AgNPs using biogenic agents has become an alternative to chemical and physical synthesis [7]. Recently, green fabrication of nanoparticles utilizing extracts from plants and microorganisms, such as actinomycetes and algae, has drawn great attention due to its simplicity and cost-effectiveness. Extracts from plants are more valuable for several reasons. Plants are easy-to-obtain and low-cost materials [8]. Additionally, plants contain a variety of phytochemicals that can act as Ag ions and Ag atoms, reducing and capping agents, respectively [9]. The synthesized AgNPs by pure phytochemicals or plant extracts have approved several biological activities, such as antifungal, antiviral, antibacterial, and anti-inflammatory effects [10,11]. Varied studies have indicated the ability of plant extracts in metal NP fabrication; for example, El-Zayat et al. [12] reported cytotoxic and antioxidant effects of varied NPs of zinc and selenium prepared using Ephedra aphylla extract. Further, garlic and ginger extracts also approved their ability in copper, silver, zinc, and iron NP formulations, which indicated significant antimicrobial activities [13].
Currently, we are focusing on Limoniastrum monopetalum as a source of active metabolites since it is known as an adapted ground cover to a variety of harsh environmental condition, such as water shortages, high light intensity, and temperatures, and can significantly grow on poor organic soil [14]. L. monopetalum is a wild small silvery herb (Plumbaginaceae); it is naturally found in coastal sands and salt marshes along Egypt's northern coast and other Mediterranean countries. Environmentally, it can stabilize coastal dunes, phytoremediate petroleum hydrocarbon degradation, and reduce heavy metals in polluted areas. Importantly, L. monopetalum could also be a potential source of biomolecules as biomedicine [15], since it is tolerant to varied stress conditions. The extracts from L. monopetalum displayed variable significant antibacterial potential since they have a great activity against multi-drug-resistant microbes belong to candida species, such as Candida glabrata and C. krusei. [16]. Recently, we published data regarding biologically active plant metabolites from Lycium shawii by an in silico study; such approach could be of interest in searching for biogenic agents as drugs. Biomolecules for a disease suppression process or their exact mode of action could be predicted using an in silico approach [17], which could be of great interest to save resources and time that are needed for in vitro and in vivo studies [18]. Recent in silico molecular docking investigations indicated that the antibacterial mechanism of NPs is the β-lactamase enzyme suppression ability [19][20][21]. Our previous study suggested that emodin, the L. shawii metabolites, is targeting the carbonic anhydrase IX enzyme and suppressing its activity [22]. Our investigation used L. monopetalum, where the leaves were extracted by water, and the final product has been used as an antibacterial agent and as a mediator in AgNP fabrication. AgNPs were characterized using different approaches, and the metabolites from the plant extract were detected using QTOF-LCMS. Further, in silico molecular target prediction was assessed for the identified biomolecules using different multiple computational approaches in a trial to detect the target of the L. monopetalum biomolecules as antibacterial agents.

Results
In the recent study, NPs were prepared using one biogenic agent, leaf extracts of L. monopetalum. The successful fabrication of AgNO 3 to AgNPs was observed by the color change to dark brown after 6 h when combined with a L. monopetalum extract in a timedependent style. AgNO 3 was incubated with the L. monopetalum extract until no more color alteration was observed, which was as an indication of the total bioconversion of Ag + ions into Ag 0 . A subsequent separation of the biogenic L-AgNPs, 1 mg/mL, was prepared for more analysis. Figure 1 reveals the distribution of the L-AgNPs size, indicating a mean size of 68.79 and a 0.349 polydispersity index (PDI). Moreover, L-AgNPs have been investigated by TEM analysis, which illustrates good dispersion, and spherical shapes have been observed, with some NPs indicating size diameters of 5 and 18 nm ( Figure 2). A SEM microscope was used for EDX analysis to find out the element composition of the NPs and is presented in Figure 3. Results indicate the presence of carbon, oxygen, and silver at their exact energy values. The morphology of L-AgNPs noted under SEM indicated some spherical nanoforms besides square ones. FTIR is an analytical method used to provide and detect organic and inorganic biomolecules that facilitate the production of NPs from Ag ions.

The Antibacterial Activity
The antibacterial action of the study agents showed high activity against all teste

The Antibacterial Activity
The antibacterial action of the study agents showed high activity against all tested bacteria, where the inhibition zones of both were greater against Gram-negative K. pneumoniae (36 ± 2.6; 19.7 ± 0.5 mm) and E. coli (37.3 ± 1.5; 25.3 ± 0.6 mm) for L-AgNPs and a L. monopetalum extract, respectively, compared with that against Gram-positive S. aureus

The Antibacterial Activity
The antibacterial action of the study agents showed high activity against all tested bacteria, where the inhibition zones of both were greater against Gram-negative K. pneumoniae (36 ± 2.6; 19.7 ± 0.5 mm) and E. coli (37.3 ± 1.5; 25.3 ± 0.6 mm) for L-AgNPs and a L. monopetalum extract, respectively, compared with that against Gram-positive S. aureus (14.3 ± 1.5; 10.7 ± 0.6 mm) and S. mutans (17.0 ± 1.0; 13.3 ± 0.6 mm) for L-AgNPs and a L. monopetalum extract, respectively. Significant differences were noted between the effect of both tested agents against all strains, as presented in Figure 5. Ampicillin was tested as a positive control. Significant variations were noted for the agents tested and bacterial strains, and p < 0.0001 was noted also for their interaction.

Prediction of Antibacterial Activity
The antibacterial activity of the identified metabolites ( Figure 6) was computed using the PASS Online (Way2Drug) web server. As summarized in Table 1, metabolite 12 demonstrated the highest predicted score (0.677), followed by metabolites 8 and 11, which possess moderate predicted activity (0.569 and 0.537, respectively). The activity predicted scores for the remaining metabolites were below 0.5, suggesting that experimental activity is insignificant.

Molecular Target Predictions
Using the SwissTargetPrediction web server, the potential molecular targets for the identified metabolites were predicted. As shown in Figure 7, metabolite numbers 1, 5, and 7-12 were predicted to act on the lyase enzyme, in particular, carbonic anhydrase II. Additionally, the predicted results suggest that multiple molecular targets could be involved in producing the beneficial antibacterial effect.

Molecular Docking Study
The molecular target prediction results indicated that the carbonic anhydrase II enzyme might be an effective biological target for enhancing the antibacterial potential of the identified metabolites. Thus, to provide additional insights into the molecular interactions of the metabolites with the CA II enzyme, a molecular docking study was conducted using the Maestro software. As summarized in Table 2, metabolite 12 exhibited the highest docking score (−10.37) with several hydrogen bonds and π-π stacking interactions with HIS94, THR199, HIP64, HIS4, TRP5, PRO201, and zinc coordination ( Figure 8). Metabolite 8 demonstrated a docking score of −7.89 with the involvement of several amino acid residues, including ASN67, GLU69, THR199, and forming zinc coordination. Our results were consistent with the antibacterial activity prediction results in which metabolites 12 and 8 demonstrated the highest predicted antibacterial activity. It is worth mentioning that the docking score of metabolite 12 was higher than the native ligand, suggesting a potential antibacterial activity for this metabolite.

Pharmacokinetic Parameters Evaluation
The evaluated pharmacokinetic properties for the identified metabolites are summarized in Table 3. Based on our results, most of the identified metabolites possessed pharmacokinetic properties that were within the recommended ranges except for metabolites 6, 8, 11, and 12. These four metabolites (6,8,11, and 12) violated Lipinski's rule of 5 on the number of hydrogen bond donors (recommended to be lower than 5) and acceptors (recommended to be less than 10), which could affect the oral absorption ( Figure 9). Moreover, metabolite 12 exhibits a molecular weight of greater than 500 daltons, which also violates the acceptable range for orally bioavailable drugs. Additionally, the effects of the metabolites on CYP isoenzymes were assessed, and none of the metabolites were predicted to inhibit CYP2C19 and CYP2C9. Meanwhile, metabolites 6, 7, and 10 were predicted to inhibit CYP1A2; metabolites 2, 4, 7, and 10 inhibit CYP2D6; and metabolites 4, 6, 7, and 10 inhibit CYP3A4.

Pharmacokinetic Parameters Evaluation
The evaluated pharmacokinetic properties for the identified metabolites are summarized in Table 3. Based on our results, most of the identified metabolites possessed pharmacokinetic properties that were within the recommended ranges except for metabolites 6, 8, 11, and 12. These four metabolites (6,8,11, and 12) violated Lipinski's rule of 5 on the number of hydrogen bond donors (recommended to be lower than 5) and acceptors (recommended to be less than 10), which could affect the oral absorption ( Figure 9). Moreover, metabolite 12 exhibits a molecular weight of greater than 500 daltons, which also violates the acceptable range for orally bioavailable drugs. Additionally, the effects of the metabolites on CYP isoenzymes were assessed, and none of the metabolites were predicted to inhibit CYP2C19 and CYP2C9. Meanwhile, metabolites 6, 7, and 10 were predicted to inhibit CYP1A2; metabolites 2, 4, 7, and 10 inhibit CYP2D6; and metabolites 4, 6, 7, and 10 inhibit CYP3A4.

Toxicity Assessment
Several organ and endpoint toxicities were assessed and evaluated for the identified metabolites using the ProTox-II web server, including hepatotoxicity, carcinogenicity, immunotoxicity, mutagenicity, and cytotoxicity. As summarized in Table 4, none of the metabolites exhibited hepatotoxicity or cytotoxicity. Meanwhile, metabolites 6 and 7 demonstrated moderate predicted carcinogenicity and mutagenicity. Furthermore, metabolites 1, 2, and 8-12 were predicted to be immunotoxic.

Prediction of Cardiac Toxicity
In addition to the organ and endpoint toxicity, it was crucial to evaluate the blockage of the hERG channel by metabolites, which could potentially lead to cardiac toxicity. Thus, the metabolites were assessed for cardiac toxicity using a pred-hERG web server. Moreover, the probability map demonstrates the involvement of the atoms in the cardiotoxicity as the green color increases with more contour lines representing greater involvement and contribution of the atoms (Table 5, probability map). As summarized in Table 5, metabolites 3, 4, 8, and 12 were predicted to be potentially cardiotoxic with confidences of 50%, 50%, 50%, and 60%, respectively. Meanwhile the remaining metabolites were predicted to be noncardiotoxic. Table 5. Cardiac toxicity of the identified metabolites using the pred-hERG web server.    Potentially cardiotoxic (+) 50%  Noncardiotoxic (−) 50%

Discussion
The study validates the formation of L-AgNPs using leaf extracts of L. monopetalum. The L-AgNPs were successfully synthesized and observed initially by changing the visible color of the mixture of a L. monopetalum extract and AgNO3 to dark brown color after 6 h from mixing of the two components. The obtained result was as an indication of the entire biotransformation and conversion of Ag + ions into Ag 0 , and the brown color is a distinctive feature of the generation of plasmon vibration excitation on the surface of L-AgNPs [29]. Further, the formation of L-AgNPs was verified by a dynamic light scattering (DLS) system to demonstrate the size distribution. The size distribution provides evidence of L-AgNP production with a mean size of 68.79 nm with a 0.349 polydispersity index (PDI). The size and density of the produced L-AgNPs suggest a lack of formation of Ag clusters or ultrasmall AgNPs [30].
The obtained L-AgNPs are further corroborated by TEM. A representative TEM image of the L-AgNPs indicates their varied size diameters of 5 and 18 nm and shows the spherical shapes of almost all AgNPs. The size of the NPs that is detected using TEM is 12 Potentially cardiotoxic (+) 60% 10 Noncardiotoxic (−) 60% 11 Noncardiotoxic (−) 50%

Discussion
The study validates the formation of L-AgNPs using leaf extracts of L. monopetalum. The L-AgNPs were successfully synthesized and observed initially by changing the visible color of the mixture of a L. monopetalum extract and AgNO3 to dark brown color after 6 h from mixing of the two components. The obtained result was as an indication of the entire biotransformation and conversion of Ag + ions into Ag 0 , and the brown color is a distinctive feature of the generation of plasmon vibration excitation on the surface of L-AgNPs [29]. Further, the formation of L-AgNPs was verified by a dynamic light scattering (DLS) system to demonstrate the size distribution. The size distribution provides evidence of L-AgNP production with a mean size of 68.79 nm with a 0.349 polydispersity index (PDI). The size and density of the produced L-AgNPs suggest a lack of formation of Ag clusters or ultrasmall AgNPs [30].
The obtained L-AgNPs are further corroborated by TEM. A representative TEM image of the L-AgNPs indicates their varied size diameters of 5 and 18 nm and shows the spherical shapes of almost all AgNPs. The size of the NPs that is detected using TEM is

Discussion
The study validates the formation of L-AgNPs using leaf extracts of L. monopetalum. The L-AgNPs were successfully synthesized and observed initially by changing the visible color of the mixture of a L. monopetalum extract and AgNO 3 to dark brown color after 6 h from mixing of the two components. The obtained result was as an indication of the entire biotransformation and conversion of Ag + ions into Ag 0 , and the brown color is a distinctive feature of the generation of plasmon vibration excitation on the surface of L-AgNPs [29]. Further, the formation of L-AgNPs was verified by a dynamic light scattering (DLS) system to demonstrate the size distribution. The size distribution provides evidence of L-AgNP production with a mean size of 68.79 nm with a 0.349 polydispersity index (PDI). The size and density of the produced L-AgNPs suggest a lack of formation of Ag clusters or ultrasmall AgNPs [30].
The obtained L-AgNPs are further corroborated by TEM. A representative TEM image of the L-AgNPs indicates their varied size diameters of 5 and 18 nm and shows the spherical shapes of almost all AgNPs. The size of the NPs that is detected using TEM is normally small compared with that noted by DLS, which is reported in our findings. Such variation could be related to the fact that both techniques were varied in their principles, where DLS generally assesses the NPs' hydrodynamic radius [31]. Besides that, the amount of the sample is much higher than that used for TEM analysis; therefore, impurities of phytochemicals that cap the NPs could also be present. Compared with other studies, the obtained TEM image also shows most of the AgNPs without aggregation as much individual particles were seen [32,33]. The absence of aggregation might be suggested by NPs' surface capping by the L. monopetalum molecules. These capping agents are also crucial to enhance NPs' biomedical functionality. The elemental assessment of NPs was conducted by SEM using EDX analysis, which approves the occurrence of carbon, oxygen, and silver atoms. The peaks detected around 3.0 keV are characteristic of metallic silver in the generated NPs. The presence of carbon and oxygen is due to the molecules of the used L. monopetalum leaf extracts. The biomolecules that are possibly found in a L. monopetalum extract could be responsible for the easy fabrication of AgNO 3 to AgNPs [34] and can be detected by the FTIR technique. Such molecules could also be accountable for the extract's biological activity. Our findings demonstrated major peaks at 1635.65 and 1635.20 cm −1 , which can be ascribed to amide I for the carbonyl stretch in proteins [35]. The peaks at 3284.38 and 3263.59 could be assigned for polyphenolic and protein peptide [36]. The occurrence of several functional groups in an NP solution suggests their role in the fabrication process. The peaks from both tested agents showed slight variation in the absorption band magnitude, which might suggest the utilization of plant metabolites in NPs fabrication. The antimicrobial effects of the L-AgNPs and L. monopetalum extract was examined against two Gram-positive bacteria (S. aureus and S. mutans) and two Gram-negative bacteria (K. pneumonia and E. coli). The result confirms the effectiveness of both the L-AgNPs and L. monopetalum extract on all tested bacteria strains. However, the inhibition effect by the obtained L-AgNPs on all selected bacteria was greater than that of the L. monopetalum extract. Moreover, both L-AgNPs and the L. monopetalum extract exhibited excellent growth inhibition against Gram-negative bacteria more than that observed for Gram-positive bacteria, which might suggest better penetration ability of the NPs in the cell of Gram-negative bacteria due to the natural barrier made of a thinner peptidoglycan layer compared with that found in the cell of Gram-positive bacteria [37]; consequently, a high effect was noted. Previous studies showed that a L. monopetalum leaf extract alone has a high level of phenolic and flavonoid compounds [13,23], and the phenolic compounds (phenolic hydroxyl groups) significantly increase the antibacterial potential by inhibiting microbial enzymes and increasing affinity to cytoplasmic membranes [38]. The strong antibacterial properties of the obtained L-AgNPs are properly due to their size and biomolecule attached over their surface [39]. Another study by Martínez-Castañon et al. stated that the smaller AgNP size, the grater their antibacterial activity [40]. E. coli shows the highest inhibition zone among the four tested bacteria (37.3 ± 1.5 mm) by L-AgNPs, which is not too far from that of the K. pneumoniae (36 ± 2.6 mm). The antimicrobial results of the L-AgNPs were in agreement with other studies that used green synthesis AgNPs [11,41,42]. Not only the NPs' size affects the antibacterial properties, but also the capping agent enhances antibacterial action [39]. Damage of bacterial cell membranes was suggested by several studies as AgNPs can cause structural changes by adhering to the negative charge lipopolysaccharides (LPS), which disrupt membrane permeability and lead to bacterial death [43,44]. Further, a study demonstrates that the alteration of the membrane in K. pneumoniae and E. coli was proposed as a mode of action of AgNPs [45]. In addition, in a report by Gopinath et al., SEM images indicated a damaged bacterial membrane after treatment with AgNPs [46]. Altering membrane functionality is the target of many antibacterial drugs. In contrast, the use of L-AgNPs is environmentally safe approach, cost-effective and overcomes the side effects associated with these antibacterial drugs. Possible antimicrobial actions for the biofabricated AgNPs might be linked to their biocompatibility, increasing the ROS production and hurting a bacterial cell [47,48].
The activity of the plant extract as an antimicrobial agent or biomediator in NP fabrication could mainly rely on its active ingredients that have been detected using QTOF-LCMS. Twelve components appeared as 12 peaks were identified from a L. monopetalum extract where: Peak 1: the value of m/z at 7.396-7.698 (retention time) has been linked to the parent compound ferulic acid [23], with the molecular formula of [C 10  Further, in this study, we examined the application of a L. Monopetalum extract as a potential antibacterial therapy by using multiple computational approaches to predict the antibacterial activity of the identified metabolites, their possible molecular targets, active site interactions, pharmacokinetic properties, and toxicity. According to PASS Online predictions, metabolite 12 (2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxo-4H-chromen-3-yl 6-O-(6-deoxy-β-Lmannopyranosyl)-β-D-glucopyranoside) and metabolite 8 (6,7-dihydroxy-5-methoxy 7-Oβ-D-glucopyranoside) demonstrated the highest antibacterial activity, and SwissADME web server results suggested the CA II enzyme as a potential molecular target for both metabolites with other targets that could be involved. Since several studies have reported that inhibitors of carbonic anhydrase enzymes could serve as a novel approach for new classes of antibacterial agents [49][50][51], the metabolites were docked into the crystal structure of human carbonic anhydrase II. Interestingly, metabolite 12 demonstrated the highest docking score, followed by metabolite 8, which both were aligned with our antibacterial activity predictions. Moreover, computational pharmacokinetic prediction results suggested that metabolite 12 violated the ROF that could affect the route of the administration, while metabolite 8 was within the recommended range for oral bioavailability. Additionally, metabolites 8 and 12 demonstrated no inhibition effect on five CYP isoenzymes, suggesting a lower tendency for drug-herbal interaction. Regarding the safety studies, the ProTox-II web server predicted that metabolites 8 and 12 could possess a potential immunotoxicity, while the pred-hERG web server predicted that these two metabolites could exhibit weak to moderate cardiac toxicity. Moreover, additional in vitro and in vivo studies need to confirm the predicted results for the metabolites. Nonetheless, the results of the computational work suggest that metabolite 12 could serve as a starting point for a potential antibacterial treatment for several human bacterial infections.

Plant Materials and Morphological Identification
Leaves were collected from 6-week-old Limoniastrum monopetalum obtained from the nursery of the Royal Commission for Riyadh City (RCRC), Riyadh, Saudi Arabia, in January 2021. Plants were characterized and confirmed at Princess Nourah bint Abdulrahman University (Riyadh, Saudi Arabia). Distilled water was used for the cleaning of plant leaves, which were then dried at room temperature and milled by a milling machine (IKA Werke GMBH and Co., Staufen im Breisgau, Germany) to attain a fine powder. The consequent material was kept in a plastic container for further analysis at room temperature.

Preparation of Limoniastrum Monopetalum Extract
An aqueous extract (2% w/v) of L. monopetalum leaves was made by adding 2 g of plant powder to 100 mL distilled water and heated at 90 • C for 15 min using water path. Then the mixture was filtered by filter paper (Whatman No. 1), which was used immediately for NP fabrication.

Preparation of AgNPs
An amount of 10 mL of an L. monopetalum extract and 90 mL of (1 mM) AgNO 3 were mixed, thereafter heated at 90 • C for 10 min. The mixture was maintained at room temperature in dark conditions. The color alteration was reported every 2 h while waiting for a final solution with a stable brown dark color, which indicated the NPs' formation by L. monopetalum (L-AgNPs). Subsequently, the resultant mixture was centrifuged for 30 min at 13,000 rpm, followed by washing the pellet two times using distilled water; then at the same conditions, the centrifugation process was repeated. Further, the pellet was preserved for drying at room temperature. Following the accomplishment of the AgNP synthesis, a NP concentration of 1 mg/mL was taken for additional use.

Characterization of L-AgNPs
L-AgNPs were characterized by various approaches applying hydrodynamic size evaluation, which was carried out using Nano ZSP Zetasizer by a dynamic light scattering (DLS) system (MAL1034318, ver 7.11, Serial Number, Malvern Instruments Ltd., Malvern, UK). The L-AgNPs structure and size distribution were carried out by transmission electron microscopy (TEM) by a TEM system (JEM-1011, Jeol, Tokyo, Japan) at an 80 kV voltage. For the detection of the presence of the element silver in the synthesized NPs and for investigating the surface and size of NPs, energy dispersive X-ray spectroscopy (EDS) was performed, applying a scanning electron microscope (SEM) (Jeol JED-2200 series).

Analysis of Surface Functional Groups
To check the capping agent from the plant extracts adsorbed on the surface of the NPs, Fourier-transform infrared spectroscopy (FTIR) analysis was taken, applying an FTIR spectrometer (Spectrum 100, PerkinElmer, Wellesley, MA, USA), at wavenumber range of 450-3500 cm −1 measured in diffuse reflectance.

Antibacterial Screening
The L. monopetalum extract and biogenic L-AgNPs were evaluated as antibacterial agents against four bacterium types: methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Streptococcus mutans, and Klebsiella pneumoniae, which were obtained from the Bio House Medical Lab in Riyadh, Saudi Arabia. The well diffusion assay was applied on the selected strain to detect the activity of the tested agents. Bacterial suspensions 0.5 McFarland concentration at 1.5 × 10 8 CFU/mL were made in saline by the straight colony suspension methodology. The selected inoculums were individually spread on the entire agar surface of the agar plates. Subsequently, holes (0.4 mm diameter each) were made and 40 µL of 1 mg/mL of L-AgNP solution was poured into the well. Additionally, a L. monopetalum extract was also tested, and ampicillin was used as a positive control. Before incubation, plates were set aside to dry under aseptic conditions for 1 h and the kept at 37 • C for 24 h. After that, the diameters of the inhibition growth area were assessed in mm. Distilled water was used as a negative control in this study.

LC-QTOF-MS Analysis for Metabolites Detection
The powder from L. monopetalum was soaked in distilled water for 48 h at 60 • C for sample preparation to LC-QTOF-MS analysis for metabolite detection. Filter paper, Whatman Grade No. 1, was used for mixture filtration, then evaporated. Methanol (1 mL) was used for dissolving the aqueous extract (1 mg). An Agilent Extend-C18 column (2.1 mm × 50 mm, 1.8 µm) was used for the separation process aided with elution gradient 0-1 min, 5% B; 1-11 min, 5%-100% B; 11-13 min, 95% B; 13-15 min, 5% B; 15-16 min, 5% B, applying 0.1% HCOOH in water (mobile phase A and 0.1% HCOOH in methanol (mobile phase B), and 10 µL and 300 µL/min were the injection volume and sample flow rate, respectively. The MS1 acquisition approach was obtained using positive mode 100 to 600 m/z as mass range. Conditions of the mass spectrometer were designed at 300 • C = gas temperature; 8 I/min = gas flow; 35 psig = nebulizer; 350 • C = sheath gas temperature, and 11. MS1 = sheath gas flow. Data were obtained by a quantitative and qualitative analysis software (Agilent MassHunter, Agilent Technologies). A mass assessment of the spectrum was detected, and LC-MS data were used for extracting the chemical features by the recursive analysis workflow and molecular feature extraction (MFE) algorithm [22].

Antibacterial Activity Prediction
Each identified metabolite was assessed for the antibacterial activity using the online web server Prediction of Activity Spectra for Substances (PASS) (http://www.pharmaexpert. ru/passonline, accessed on 20 June 2022). The web server predicts the activity of small molecules based on the database, and the results are represented by the probability of the compound possessing activity (P a ) or inactivity (P i ) at a variety of pharmacological activities. The small molecule is predicted to be highly active experimentally if P a is greater than 0.7, while the range of 0.7 to 0.5 predicts moderate activity, and lower than 0.5, the biological effect is negligible [52].

Target and Pharmacokinetic Prediction
Molecular target predictions and multiple pharmacokinetic parameters were computed by applying the SwissADME web server (http://www.swissadme.ch/index.php, accessed on 20 June 2022) [53]. The Simplified Molecular Input Line Entry System (SMILES) of each metabolite was used as an input to generate these predictions. Several pharmacokinetic properties were computed, including physicochemical parameters, lipophilicity, absorption, distribution, metabolism, and druglikeness according to Lipinski's rule of five (ROF) [54,55].

Molecular Docking Study
Molecular docking for identified metabolites was performed using the Maestro Schrödinger software (Schrödinger, New York, NY, USA). The crystal structure of human carbonic anhydrase II (hCA II, PDB ID: 5AML, resolution 1.36 Å) was prepared using the protein preparation wizard tool in Maestro. The chemical structures were arranged by the LigPrep tool, and the grid of the active site was created using the receptor grid generation tool in Maestro. The docking results were subjected to quantitative (docking scores ranking) and qualitative (molecular interactions involved) analysis.

Cardiac Toxicity Prediction
The blockage of hERG K(+) channels can lead to fatal cardiac arrhythmia and toxicity; thus, the metabolites were assessed using the pred-hERG 4.2 web server (http: //predherg.labmol.com.br, accessed on 21 June 2022). The results are presented as probability prediction (cardiotoxic versus noncardiotoxic) with confidence and probability maps to visualize the contribution of each atom to the predicted toxicity. The green color (the darker the color with more lines suggests higher involvement in the cardiac toxicity) in the probability map indicates the involvement of the atom in the blockage of hERG, while the pink color indicates the contribution to lowering the cardiac toxicity, and the gray color indicates no involvement in the toxicity [57].

Statistical Analysis
All assessments were carried out in triplicate. Data were presented as mean ± standard deviation (SD). Differences among the investigated components were statistically gained by two-way analysis of variance (ANOVA) by the Prism 9.1 software (GraphPad Software Inc., La Jolla, CA, USA).

Conclusions
The aqueous extract of L. Monopetalum revealed an ability in AgNP fabrication, and the in vitro antibacterial effect was approved for both agents. The inhibitory action of the plant extract and AgNPs was higher against Gram-negative bacteria than that against Grampositive-tested bacteria. Twelve biomolecules were identified in the L. Monopetalum extract by QTOF-LCMS analysis, and our computational results suggested that L. Monopetalum metabolites could hold promising antibacterial activity with minimal toxicity and an acceptable pharmaceutical profile. Additional experimental work needs to confirm the computed antibacterial, ADME, and toxicity predictions for the identified metabolites. Moreover, isolation, in vitro, and in vivo antibacterial assessment for each metabolite present in a L. Monopetalum extract could be the future direction to identifying new antibacterial agents from natural products.