Effect of Natural Adenylcyclase/cAMP/CREB Signalling Activator Forskolin against Intra-Striatal 6-OHDA-Lesioned Parkinson’s Rats: Preventing Mitochondrial, Motor and Histopathological Defects

Parkinson’s disease (PD) is characterised by dopaminergic neuronal loss in the brain area. PD is a complex disease that deteriorates patients’ motor and non-motor functions. In experimental animals, the neurotoxin 6-OHDA induces neuropathological, behavioural, neurochemical and mitochondrial abnormalities and the formation of free radicals, which is related to Parkinson-like symptoms after inter-striatal 6-OHDA injection. Pathological manifestations of PD disrupt the cAMP/ATP-mediated activity of the transcription factor CREB, resulting in Parkinson’s-like symptoms. Forskolin (FSK) is a direct AC/cAMP/CREB activator isolated from Coleus forskohlii with various neuroprotective properties. FSK has already been proven in our laboratory to directly activate the enzyme adenylcyclase (AC) and reverse the neurodegeneration associated with the progression of Autism, Multiple Sclerosis, ALS, and Huntington’s disease. Several behavioural paradigms were used to confirm the post-lesion effects, including the rotarod, open field, grip strength, narrow beam walk (NBW) and Morris water maze (MWM) tasks. Our results were supported by examining brain cellular, molecular, mitochondrial and histopathological alterations. The FSK treatment (15, 30 and 45 mg/kg, orally) was found to be effective in restoring behavioural and neurochemical defects in a 6-OHDA-induced experimental rat model of PD. As a result, the current study successfully contributes to the investigation of FSK’s neuroprotective role in PD prevention via the activation of the AC/cAMP/PKA-driven CREB pathway and the restoration of mitochondrial ETC-complex enzymes.


Introduction
Parkinson's disease (PD) is a neurological condition that causes neurodegeneration as well as motor impairments (tremors, muscle weakness, akinesia and postural instability) [1]. Due to dopamine (DA) deficiency, Parkinson's disease (PD) is characterised by Lewy bodies On the first, ninth, fifteenth and twenty-first day of the procedure, the percentage of activity (fall-off time) on the grip strength apparatus was measured to evaluate the muscle strength of the rats. The naive and naive + FSK (45 mg/kg p.o.) groups showed no statistically significant differences. In contrast to the naive and naive + FSK groups, the chronic administration of 6-OHDA (8 µg/2 µL/unilateral) caused a gradual decrease in grip strength as measured by the time required to hold a metal wire. Moreover, the chronic administration of FSK at doses of 15, 30 and 45 mg/kg revealed a dose-dependent grip strength improvement in comparison to the 6-OHDA-treated group [F 15,90 = 1361.76; ANOVA (two-way); p < 0.01] ( Figure 2). In rats with 6-OHDA-induced PD, treatment with a high dose of FSK (45 mg/kg) both lengthens the time needed to grasp a metal wire and makes restoring grip strength more effective.

FSK Improved Memory Retention in the Experimental Model of PD
The transfer latency (TL) task was used to test learning and memory in rats on the twentieth, twenty-first and twenty-second days of the protocol schedule. This study found no statistically significant differences between the naive and naive + FSK groups. In contrast to the naive and naive + FSK groups, however, the chronic injection of 6-OHDA (8 µg/2 µL/unilateral) significantly reduced the TL. In addition, chronic FSK (15, 30 and 45 mg/kg) showed a dose-dependent improvement over 6-OHDA [F 5,25 = 2.119; ANOVA (one-way); p < 0.01] (Figure 3). The administration of a high dose of FSK (45 mg/kg) demonstrates a significant improvement in memory restoration and increases the TL in rats exposed to 6-OHDA, which causes PD.

FSK Restored Neuromuscular Coordination in the Experimental Model of PD
The number of slips on the NBW and neurological scores were recorded on days 1 and 21 of the protocol to assess the rats' mobility impairment. The naive and naive + FSK (45 mg/kg p.o.) groups were compared, and there was no statistically significant difference between them. However, compared to the naive and naive + FSK groups, there was a gradual rise in the number of slips after the chronic administration of 6-OHDA  statistically significant differences. In contrast to the naive and naive + FSK groups, the chronic administration of 6-OHDA (8 μg/2 μL/unilateral) caused a gradual decrease in grip strength as measured by the time required to hold a metal wire. Moreover, the chronic administration of FSK at doses of 15, 30 and 45 mg/kg revealed a dose-dependent grip strength improvement in comparison to the 6-OHDA-treated group [F15,90 = 1361.76; ANOVA (two-way); p < 0.01] (Figure 2). In rats with 6-OHDA-induced PD, treatment with a high dose of FSK (45 mg/kg) both lengthens the time needed to grasp a metal wire and makes restoring grip strength more effective.

FSK Improved Memory Retention in the Experimental Model of PD
The transfer latency (TL) task was used to test learning and memory in rats on the twentieth, twenty-first and twenty-second days of the protocol schedule. This study found no statistically significant differences between the naive and naive + FSKgroups. In contrast to the naive and naive + FSK groups, however, the chronic injection of 6-OHDA (8 μg/2 μL/unilateral) significantly reduced the TL. In addition, chronic FSK (15,  (one-way); p < 0.01] (Figure 3). The administration of a high dose of FSK (45 mg/kg) demonstrates a significant improvement in memory restoration and increases the TL in rats exposed to 6-OHDA, which causes PD.

FSK Restored Neuromuscular Coordination in the Experimental Model of PD
The number of slips on the NBW and neurological scores were recorded on days 1 and 21 of the protocol to assess the rats' mobility impairment. The naive and naive + FSK (45 mg/kg p.o.) groups were compared, and there was no statistically significant difference between them. However, compared to the naive and naive + FSK groups, there was a gradual rise in the number of slips after the chronic administration of 6-OHDA (8 μg/2 μL/Unilateral). Moreover, the chronic administration of FSK at doses of 15, 30 and 45   Data provided as mean ± SD (n = 6) followed by ANOVA (two-way) with post-hoc Bonferroni test. * 6-OHDA (p < 0.01) compared with naive and naive + FSK; # FSK15, FSK30, FSK45 (p < 0.01) compared with 6-OHDA; #@ FSK45 (p < 0.01) compared with FSK30, FSK15. (b) FSK reduced the neurological score using the beam crossing task (BCT) in the experimental model of PD. Data provided as mean ± SD (n = 6) followed by ANOVA (two-way) with post-hoc Bonferroni test. * 6-OHDA (p < 0.01) compared with naive and naive + FSK; # FSK15, FSK30, FSK45 (p < 0.01) compared with 6-OHDA; #@ FSK45 (p < 0.01) compared with FSK30, FSK15.

FSK Improved Locomotion in the Experimental Model of PD
Rats' locomotor activity (LA) was evaluated using open field apparatus on the protocol schedule's first, eighth, fourteenth and twentieth days. The naive and naive + FSK groups displayed no statistically significant differences. Conversely, compared to the naive and naive + FSK groups, the chronic treatment of 6-OHDA (8 μg/2 μL/unilateral) significantly reduced locomotor movement in rats. Furthermore, there was a substantial improvement in locomotion demonstrated following the chronic administration of FSK (15, 30 and 45 mg/kg) in a dose-dependent manner in comparison to the 6-OHDA treated group [F15,90 = 166.49; ANOVA (two-way); p < 0.01] ( Figure 5). In rats, a high dose of FSK (45 mg/kg) improves and promotes locomotor movement against 6-OHDA-induced PD. Data provided as mean ± SD (n = 6) followed by ANOVA (two-way) with post-hoc Bonferroni test.  According to the protocol, akinesia on the stepping apparatus was performed on the seventh, fourteenth and twenty-first days to assess the impairment in voluntary movement. There was no statistically significant difference between the naive and naive + FSK (45 mg/kg p.o.) groups. Compared to the naive and naive + FSK groups, there was a steady reduction of voluntary movement after the chronic injection of 6-OHDA (8 μg/2 Figure 5. FSK improved locomotion using the open-field test in the experimental model of PD. Data provided as mean ± SD (n = 6) followed by ANOVA (two-way) with post-hoc Bonferroni test. * 6-OHDA (p < 0.01) compared with naive and naive + FSK. # FSK15, FSK30, FSK45 (p < 0.01) compared with 6-OHDA; #@ FSK45 (p < 0.01) compared with FSK30, FSK15.

FSK Restored Voluntary Movement in the Experimental Model of PD
According to the protocol, akinesia on the stepping apparatus was performed on the seventh, fourteenth and twenty-first days to assess the impairment in voluntary movement. There was no statistically significant difference between the naive and naive + FSK (45 mg/kg p.o.) groups. Compared to the naive and naive + FSK groups, there was a steady reduction of voluntary movement after the chronic injection of 6-OHDA (8 µg/2 µL/unilateral).  Step Length During Stepping Test (cm)

FSK Improved Motor Coordination in the Experimental Model of PD
The rotarod task was used to test motor coordination in rats on the first, s fourteenth and twenty-first days of the protocol schedule. The naive and naive groups showed no statistically significant differences. The rats' motor coordinat nificantly declined after chronic 6-OHDA (8 μg/2 μL/unilateral) treatment. Mo there was a dose-dependent improvement in neuromuscular coordination a long-term administration of FSK (15, 30 and 45 mg/kg) compared to the 6-OHDA group [F15,90 = 347.89; ANOVA (two-way); p < 0.01] (Figure 7). When g 6-OHDA-induced PD rats, a high dose of FSK (45 mg/kg) significantly improve dination restoration and increases motor movement.

FSK Improved Motor Coordination in the Experimental Model of PD
The rotarod task was used to test motor coordination in rats on the first, seventh, fourteenth and twenty-first days of the protocol schedule. The naive and naive + FSK groups showed no statistically significant differences. The rats' motor coordination significantly declined after chronic 6-OHDA (8 µg/2 µL/unilateral) treatment. Moreover, there was a dose-dependent improvement in neuromuscular coordination after the long-term administration of FSK (15, 30 and 45 mg/kg) compared to the 6-OHDA-treated group [F 15,90 = 347.89; ANOVA (two-way); p < 0.01] (Figure 7). When given to 6-OHDA-induced PD rats, a high dose of FSK (45 mg/kg) significantly improves coordination restoration and increases motor movement.   Mitochondrial enzyme complex I (NADPH dehydrogenase), complex II (succinate dehydrogenase (SDH)), and complex V (ATP) activity were measured in the rat brain

FSK Mediated the Restoration of Cellular and Molecular Alterations in the Experimental Model of PD FSK Restored the ETC-Complexes (I, II, V) Mitochondrial Enzyme Levels in the Experimental Model of PD
Mitochondrial enzyme complex I (NADPH dehydrogenase), complex II (succinate dehydrogenase (SDH)), and complex V (ATP) activity were measured in the rat brain homogenate at the end of the experimental schedule. No significant differences were observed between the naive and naive + FSK (45 mg/kg p.o.) groups. Nonetheless, compared to the naive and naive + FSK groups, rat complex I, II and V activity was significantly reduced after the chronic injection of 6-OHDA (

FSK Improved cAMP and CREB Protein Levels in the Experimental Model of PD
At the end of the protocol schedule, molecular markers (cAMP and CREB) were measured in the rats' brain homogenate. The naive and naive + FSK (45 mg/kg p.o.) groups showed no statistically significant differences. In contrast to the naive and naive + FSK groups, the amount of cAMP and CREB proteins gradually decreased following the continuous injection of 6-OHDA (8 µg/2 µL/unilateral).

FSK Restored Neurotransmitters in the Experimental Model of PD
Neurotransmitter levels (DA, glutamate, GABA and Ach) were measured in rat brain homogenates at the end of the experimental schedule. The naive and naive + FSK (45 mg/kg p.o.) groups showed no statistically significant difference. However, GABA and DA levels gradually decreased. In addition, the prolonged injection of 6-OHDA  Staining of the striatal brain sections with haematoxylin and eosin (H and E) reveals changes in neuronal cell density, dopaminergic neuron loss and the presence of Lewy bodies. Dopaminergic neuron loss causes neuronal degeneration and the formation of Lewy bodies in the rat brain. On the slides, the blue arrow represents neuronal density, while the black arrow represents the formation of Lewy bodies.
(A) The diagram depicts the naive group, which has a high density of neuronal cells.
(B) The diagram depicts the FSK per se group, and there was no significant difference in neuronal cell density between the naive and naive + FSK groups.

FSK Prevents Histopathological Alterations in a Striatal Brain Region in the Experimental Model of PD
After the protocol schedule, histopathology was seen on the stained sectioned slides of the striatum region of the rat brains. The naive and naïve (13A) + FSK (45 mg/kg p.o.) (13B) groups showed no significant histopathological changes. However, after the continuous injection of 6-OHDA (8 μg/2 μL/unilateral) (13C), there were progressive increases in Lewy body formation, neurodegeneration and apoptosis compared to the naive and naive + FSK treated groups. Moreover, the continuous treatment of FSK at doses of 15, 30 and 45 mg/kg (13D,E,F) decreased the development and deposition of Lewy bodies in a dose-dependent manner compared to the 6-OHDA-treated group. FSK at high doses (45 mg/kg) reverses 6-OHDA-induced PD in rats by decreasing degeneration and apoptosis while restoring normal Lewy body development ( Figure 13A-F). Staining of the striatal brain sections with haematoxylin and eosin (H and E) reveals changes in neuronal cell density, dopaminergic neuron loss and the presence of Lewy bodies. Dopaminergic neuron loss causes neuronal degeneration and the formation of Lewy bodies in the rat brain. On the slides, the blue arrow represents neuronal density, while the black arrow represents the formation of Lewy bodies.

Discussion
6-OHDA is a neurotoxin that inhibits complexes I and IV, causing mitochondrial dysfunction and, eventually, the death of catecholaminergic neurons (DA, adrenaline and noradrenaline) [35]. It does not penetrate the blood-brain barrier and is injected directly into the brain using stereotaxic surgery. An increasing body of research has shown that 6-OHDA induces motor impairment [21].
MWM and EPM were employed in this study to investigate memory and cognitionrelated behavioural activities. Rats given 6-OHDA had considerable learning and memory impairment compared to the naive and FSK-treated groups. In rats, 6-OHDA induced abnormal motor and behavioural patterns such as bradykinesia, muscular weakness, postural imbalance, dyskinesia and rigidity. PD rats with impaired learning and memory due to 6-OHDA administration exhibited aberrant motor test performance on the EPM, rotarod, stepping test, MWM, beam crossing and grip strength. According to this research, the administration of 6-OHDA to rats led to a wide range of neurobehavioral abnormalities, such as cognitive impairment, psychosocial problems and motor impairments. FSK decreased TL while increasing time spent in the target quadrant, grip strength and motor activities in a dose-dependent manner. Several neurotransmitter processes, including dopaminergic, glutamatergic, cholinergic and GABAergic, exhibited significant neurochemical alterations after the unilateral injection of 6-OHDA into the striatum of rats [36]. The experimental validation of 6-OHDA lesion model is influenced by biochemical changes (DA depletion, neurotransmitter changes), nigrostriatal pathological changes (SNpc cells loss > VTA), and forelimb akinesia, as well as mitochondrial dysfunction, oxidative stress and inflammation [37].
DA neurodegeneration in the SN and striatal axonal loss resulted in DA deficiency in basal ganglia (BG) region [38]. A disruption in the function of the mitochondrial respiratory chain, which also increases free radical production and lowers ATP, impedes oxidative phosphorylation [39].
The level of cAMP decreases when the mitochondrial respiratory chain is disturbed, resulting in the loss of ATP [40]. Adenylcyclase is stimulated as neurotransmitters bind to GPCRs, producing cyclic AMP (cAMP) [41]. Additionally, the cAMP/PKA signalling pathway aids in developing long-term memories [42]. As a direct activator of adenylate cyclase (AC), FSK protects neurons from mitochondrial dysfunction by increasing cAMP, activating PKA and CREB. There is evidence that FSK increases AC activity in the hippocampus, prefrontal cortex and striatum regions [43].
CREB is a transcription factor activated by cAMP/PKA in learning and memory formations [44]. Acetylcholine esterase, often known as AChE, is the enzyme in people with Parkinson's disease that is responsible for maintaining healthy cholinergic function [45]. In the current experiment, FSK could minimise the amount of AChE observed in the PD brain.
Direct SN lesions produce DAergic neuron degeneration and tyrosine-hydroxylase (TH) deficiency in striatal neuron terminals. Additional respiratory inhibition and oxidative stress are caused by direct interactions with complexes I and IV of the mitochondrial respiratory chain. Consequently, oxidative stress and mitochondrial respiratory failure are the hallmarks of dopaminergic degeneration [46]. Furthermore, reduced levels of free radical-scavenging enzymes (GPx and CAT) and higher levels of oxidative stress were also observed in PD conditions [47,48]. FSK administration resulted in a considerable increase in the antioxidant enzymes SOD, CAT and total GSH, as well as a significant decrease in the amount of the oxidised free radical identified as H 2 O 2 . 6-OHDA inhibits the mitochondrial respiratory chain complexes I and IV, which are responsible for the formation of free radicals [49]. Our studies also significantly restored complex I, II and V activities after long-term FSK treatment.
People with Parkinson's disease have been reported to have increased levels of Creactive protein (CRP) and inflammatory cytokines [50]. This is because activated glial cells cause neurotoxicity by releasing pro-inflammatory cytokines, including TNF-and IL-6. In 6-OHDA-treated rat brains, FSK therapy dramatically decreased inflammatory cytokines while increasing enzymatic LDH and MDA levels.
Cell death in the SNpc area of the brain is caused by the generation of Lewy bodies. H and E-stained brain slices revealed alterations in neuronal cell density, dopaminergic neuron loss and the presence of Lewy bodies [36]. Neuronal degeneration and the development of Lewy bodies are caused by the loss of dopaminergic neurons after 6-OHDA exposure. According to this study, FSK reduces Lewy body-induced neuronal cell degeneration, DAergic neuron repair and cell death.
This study's findings show that, compared to naive controls, prolonged treatment with naive + FSK had no effect on behavioural or biochemical tests in rats. FSK at 15, 30 and 45 mg/kg p.o. treated rats performed better than 6-OHDA-treated rats on a variety of behavioural and biochemical markers. FSK-protected neurons from 6-OHDA-mediated neurotoxicity were prevented by striatal histopathological alterations. In addition, stimulating the AC was found to be a more effective method for minimising the neurotoxicity produced by 6-OHDA. The cAMP/PKA/CREB pathway may play a role in the neuroprotective and regenerative effects of AC activation. However, research has revealed that CREB levels decline significantly as Parkinson's disease progresses. Based on these findings, we conclude that FSK, a direct AC activator, enhances the neuroprotective and mitochondrial complex I, II and V restorative activities of cAMP-mediated CREB.
Based on our findings, we suggest that FSK could be utilised to treat symptoms similar to Parkinson's disease. The fundamental technique, however, must be validated through adenylcyclase gene research employing knock-in and knock-out procedures. These findings will mostly be supported by cellular marker tests such as Western blot analysis and immunohistochemistry. According to our findings, conventional drugs are more successful when combined with FSK and could be employed in Parkinson's disease therapy. We also urge more research to better understand the connection between conventional drugs and PD therapies.
There are limitations to this study. The current findings are preliminary work to reinforce future investigation about the role of FSK in the progression of Parkinson's disease. The small number of animals in each group after conducting numerous behavioural and neurochemical parameters is another concern, and further research must involve a larger number of animals in each group. In the current study, a sham control group was taken as a naive control. Furthermore, the precise location of the cannula implantation must be verified with gross pathological examinations using the whole brain and brain sectioning. The correct location of the cannula should have been confirmed by staining of the striatum; however, the behavioural changes (LA, fall-off time, rotation) suggest that the cannula are most likely positioned in the correct area as all of the 6-OHDA group display changes in these parameters on days 7-8 which are reversed by FSK treatment.

Experimental Animals
The Rajendra Institute of Technology and Sciences in Sirsa, Haryana, India, provided 36 male Wistar rats (22 weeks old; 220-250 g) for the experiment. They were housed in polyacrylic cages with soft bedding and a wire mesh top for two weeks to let them become used to the laboratory environment. A standard environment was maintained, with a temperature of 22 ± 2 • C, a humidity of 65 to 70%, a 12-h light-dark cycle and unlimited access to food and water. The

Drugs and Chemicals
Sigma-Aldrich was contacted for the acquisition of 6-OHDA (St. Louis, MO, USA). This batch of FSK came from the Indian company BAPEX Pharma, which is based in New Delhi. Elabscience, China supplied the ELISA kits (MBP, cAMP, CREB, TNF-, IL-1) (ELab Sciences, Wuhan, China). The rest of the substances employed in the experiment are also important to research. Before use, the drug and chemical solutions were freshly prepared. 6-OHDA was dissolved in 0.5 M HCL and DMSO and delivered at a dose of 8 g/2 µL/unilateral for 1-7 days. FSK was dissolved in water (containing 2% ethanol) and administered orally [21,26].

Animal Model of 6-OHDA-Induced PD in Rats
All animals in experimental groups 3, 4, 5 and 6 were anaesthetised with Ketamine (70 mg/kg b.w) (i.p.) and Xylazine (10 mg/kg b.w) (s.c.). After shaving the scalp hair, we cut about 1-2 cm along from the anterior to the posterior to expose the scalp to measure and mark the striatum coordinates. Each animal was mounted on a stereotaxic apparatus, the skin overlying the skull was cut to expose it, and the coordinates for the striatum were measured accurately from bregma (anterior-posterior 0.5 mm, lateral 2.5 mm, 5 mm dorso-ventral from dura) with the tooth bar set at 0 mm (stereotaxic co-ordinates followed by Paxinos and Watson, 2013, 7th edition). After that, all animals in the experimental groups were lesioned by injecting (8 μg/2 μL 6-OHDA in 0.1% ascorbic acid-saline) into the right striatum. The cannula was inserted into the hole and fixed using

Animal Model of 6-OHDA-Induced PD in Rats
All animals in experimental groups 3, 4, 5 and 6 were anaesthetised with Ketamine (70 mg/kg b.w) (i.p.) and Xylazine (10 mg/kg b.w) (s.c.). After shaving the scalp hair, we cut about 1-2 cm along from the anterior to the posterior to expose the scalp to measure and mark the striatum coordinates. Each animal was mounted on a stereotaxic apparatus, the skin overlying the skull was cut to expose it, and the coordinates for the striatum were measured accurately from bregma (anterior-posterior 0.5 mm, lateral 2.5 mm, 5 mm dorso-ventral from dura) with the tooth bar set at 0 mm (stereotaxic co-ordinates followed by Paxinos and Watson, 2013, 7th edition). After that, all animals in the experimental groups were lesioned by injecting (8 µg/2 µL 6-OHDA in 0.1% ascorbic acid-saline) into the right striatum. The cannula was inserted into the hole and fixed using dental cement.
Then, the incision was stitched using absorbable suturing thread and a sterile needle. Cannula-implanted rats were housed individually in polyacrylic cages with warm clothes to avoid hypothermia-like conditions in surgical rats, and the room temperature was kept at 25 ± 3 • C. The rats were given special attention until they regained spontaneous movement following surgery. After surgery, milk and glucose water were kept in the cages to minimise physical trauma. To avoid sepsis, rats were given gentamycin (35 mg/kg) intraperitoneally (i.p.) for 3 days. On the sutures, lignocaine gel was applied to relieve pain. The sutures were dusted with Neosporin powder to avoid bacterial infection. The injections were made stereotaxically with the help of a Hamilton syringe through the burr holes made on the skull surface in the experimental groups. The injection rate was 1.0 µL/min, and the Hamilton syringe was kept in place for an additional 1 min before being slowly retracted [13].

Morris Water Maze (MWM) for Memory and Cognition
The animals' capacity to learn and retain spatial information was evaluated on days 5, 10 and 15 using the MWM. The animals were trained for 4 days (days 1, 2, 3 and 4) before the final trial, i.e., on days 5, 10 and 15 of the protocol schedule. Each trial had a ceiling time of 120 s and an interval between two trials of roughly around 120 s. TSTQ (time spent in the target quadrant zone) was also measured to find out how long it takes to find the secret platform. The amount of time spent in the "targeted" quadrant was linked to how well newly learned information was preserved in the long-term memory [51].

String Test for Grip Strength
The animals' grip strength was measured using a grip strength meter (Hague Ave, Columbus, OH 43204 USA). A grip strength test was performed on day 1, day 9, day 15 and day 21 as per the protocol. When the paw was released from the platform, the highest force applied was measured in kilogram-force (Kgf) [52].

Elevated Plus Maze Test (EPM) Task for Working Memory
The rats' learning and memory were assessed using the EPM, a behavioural model in which the stimulus is external to the body. EPM was carried out across 2 days, on the 13th and 14th days of the protocol schedule. On the 13th day, the animals were positioned on the open ends of their arms, with their backs on the centre platform. TL was measured on the 13th day, related to the acquisition trial, and again 24 h later on the 14th day of the protocol schedule, which corresponded to the retention trial. The time it took the animal to move into any of the covered arms with all four legs was defined as TL [53].

Beam Crossing Task (BCT) for Neuromuscular Coordination
Gait irregularities and the number of times the foot slipped while walking was measured using the NBW test device. Before the experiments, all animals were trained for 7 days on the NBW. The length of time it took the animal to get from one location to another and the number of falls it experienced were recorded for each trial [54].

Actophotometer for Locomotion
The animals' ability to move about of their own accord was measured in the open field weekly. The open field apparatus consists of 25 black and white boxes that measured how many squares were crossed during movement and how much this increased from the 1st to the 21st day. The duration of the experiment was 5 min [55].

Stepping Test for Akinesia
Forelimb akinesia was assessed by a stepping test in rats 3 weeks post-lesion. Each forelimb's time to initiate stepping, the length of each stride and the adjustment time were measured throughout the stepping test. Experiments were performed on days 7, 14 t and 21 as specified in the protocol. The experimenter measured the time, not the fixed movement of the forelimb. The amount of time that passed before the rat started moving the forelimb that the experimenter had not fixed was measured. An initiation time of this length was established, with 180 s serving as the cut-off. When the rats were moved forward and backward along the table at a rate of 90 cm every 5 s, their free forelimbs had to step with the movement of the investigator to maintain balance. The adjusting steps were recorded as the total number of free forepaw steps while maintaining balance. The two arms were put to the test alternately [56].

Rotarod Test for Grip and Neuromuscular Strength
Animals were tested on their motor skills and coordination using the rotarod device. Before undergoing the actual experimentation, each animal was trained for 7 days. The experiment was conducted on January 1st, 7th, 14th and 21st when animals were placed on the spinning rod at a speed of 25 revolutions per minute. The time was limited to a maximum of 180 s [57].

Quantification of Biochemical Parameters
On the 22nd day of the treatment, the rats were sacrificed by decapitation, and their brains were removed, washed and stored in an ice-cold isotonic saline solution. At room temperature, the brain was homogenised 10 times (w/v) with 0.1 M potassium phosphate buffer (7.4). The supernatant was collected after centrifuging the homogenate for 15 min at 10,000× g, and aliquots were used for biochemical examination [58].

Estimation of Cellular and Molecular Markers Analysis of Myelin Basic Protein (MBP) Level
A commercial ELISA kit (E-EL-R0010/MBP/E lab sciences; Wuhan, Hubei, China) was used to detect the amount of MBP in the sample. The calculated values were displayed as a function of protein content in µg/mg [59].

Estimation of Mitochondrial ETC-Complexes' Enzymes Activity Preparation of Crude Mitochondrial Fraction from Rat Whole Brain Homogenate
A crude form of the mitochondrial fraction was prepared. The pellet formed during the PMS preparation procedure was mixed in a 1:10 proportion with a 0.1 M sodium phosphate buffer (pH 7.4) after 60 min of stirring at 4 • C. For 30 min, the mixture was centrifuged at 16,000× g at 0 • C. The pellets were resuspended in a 250 mmol/L sucrose solution added to the original buffer. The crude mitochondrial fraction in the buffered sucrose solution was prepared three times using the centrifugation and resuspension method [60].

Analysis of Complex-1 Enzyme Levels (NADPH Dehydrogenase)
The post-nuclear supernatant was collected from mechanically homogenised sections of adult rat brains in a buffer containing 10 mMTris-HCl pH 7.2, 225 mannitol, 75 saccharose and 0.1 mM EDTA after centrifugation (600 g, 4 • C, 20 min). The rate of NADH oxidation at 340 nm was utilised to estimate Complex I activity at 37 • C for 3 min by spectrophotometry [61].

Analysis of Complex-II Enzyme Level (Succinate Dehydrogenase/SDH)
The gradient fraction of 50 µL of the homogenate was mixed with 0.3 mL of sodium succinate solution. Following a 10-20 min incubation period at 37 • C, 0.1 mL of piodonitrotetrazolium violet (INT) solution was added to the mixture, which was then incubated for another 10 min. After adding 1 mL of a 5:5:1 (v/v/w) solution of ethyl acetate, ethanol and trichloroacetic acid (TCA) to the reaction mixture and centrifuging at 15,000× g rpm for 1 min, the absorbance at 490 nm was measured (Shimadzu, UV-1700). The amount of lowered INT mol/mg protein was reported [28].

Analysis of Complex-V Enzyme Level (ATPase)
Small homogenate aliquots were sonicated in ice-cold per-chloric acid to deactivate the ATPases (0.1 N). Before analysis, ATP-containing supernatants were centrifuged (14,000× g, 4 • C, 5 min), neutralised with 1N NaOH and frozen at −80 • C. We measured the amount of ATP in the supernatants using a reverse-phase high-performance liquid chromatography (HPLC) device (Perkin Elmer). A 100 mM KH2PO4 buffer solution (pH 6.0), a flow rate of 1.2 mL/min, a column temperature of 25 • C and a detection wavelength of 254 nm were used in separation. The ATP reference solution was created using the same approach as the dissolving standard (Sigma, St. Louis., MO, USA) [62].

Estimation of cAMP and CREB Levels
The immunoreactivity of cAMP and CREB was measured in homogenised tissue using ELISA's commercial kits (E-EL-R0056/cAMP; E-EL-R0289/CREB, Elabsciences, Wuhan, Hubei, China). The values were expressed as pg/mL [25].

Neurotransmitter Measurement Neuronal GABA and Glutamate Levels
The amounts of GABA and glutamate were determined using HPLC and an ECD. A standard Waters system with a high-pressure isocratic pump, a 20 µL manual sample injector valve, a C18 reversed-phase column and a UV detector was integrated with an ECD. The mobile phase comprised 22% methanol, 25% EDTA and 100% anhydrous disodium hydrogen phosphate (pH 6.5). The experimental electrochemical conditions were +0.65 V and the sensitivity ranged from 5 to 50 nA. The separation flow rate was 1.2 mL/min, and the column temperature was maintained at 40 • C. To manually inject samples (20 µL), a rheodyne valve injector was employed. On the day of the experiment, frozen brain samples were thawed and homogenised in 0.2 M perchloric acid. After that, the samples were exposed to an anteroposterior 12,000 g for 15 min. The supernatant was injected into the HPLC sample injector after being derivatised with OPA/-ME (o-pthalaldehyde/mercaptoethanol) and filtered using 0.22 m nylon filters. Data were recorded and analysed using Waters HPLC's Breeze (version 3.2). The concentrations of amino acids were calculated by extrapolating a standard curve with a concentration standard between 10 and 100 ng/mL. A naive group was used for comparison, and the results were provided as a percentage [66].
Neuronal DA Level DA levels in the brain were measured using high-performance liquid chromatography (HPLC) with an electrochemical detector (ECD). A Waters standard system was used during the research. This system includes a high-pressure isocratic pump, a 20 µL manual sample injector valve, a C18 reversed-phase column and an ECD. The mobile phase was an acetonitrile-sodium citrate buffer (pH 4.5) mixture (87:13, v/v). The sodium citrate buffer was composed of 10 mM citric acid, 25 mM NaH2HPO4, 25 mM EDTA (ethylene diamine tetra-acetic acid) and 2 mM 1-heptane sulfonic acid. The electrochemical parameters of the experiment were +0.75 V, with a 5-50 nA sensitivity. A flow rate of 0.8 mL/min was applied for this separation. Each sample was injected by hand at a volume of 20 µL. The experiment's homogenising solution contained 0.2 M perchloric acid, which was used to defrost the frozen brain samples before homogenisation. After that, the samples were centrifuged at 12,000× g for 5 min. The supernatant was filtered through 0.22-mm nylon filters before being injected into the HPLC sample injector. Breeze software was used to collect and analyse the data [67].

Neuronal (Ach) Level
A diagnostic kit measured Ach levels (Krishgen diagnostics, Mumbai, India). All reagents and samples were made according to the instructions included in the kit. A microtiter plate was used to take readings of the optical density of the reaction mixture at 540 nm [59].

Assessment of Oxidative Stress Markers Analysis of Lactate Dehydrogenase (LDH) Level
A diagnostic kit (Coral Diagnostics, India) was used to measure the lactate dehydrogenase activity in a rat brain homogenate; the results were reported in international units per litre (IU/L) [68].

Analysis of Superoxide Dismutase (SOD) Level
The spectrophotometric detection of the auto-oxidation of epinephrine at a pH of 10.4 was used to calculate SOD activity. This technique found 0.02 mL of epinephrine in the brain homogenate supernatant (0.2 mL). The absorbance was determined using spectrophotometry at 480 nm after 5 min. SOD activity was reported as % of the control value [55].

Analysis of Catalase Level
An assay for CAT activity was performed by adding 0.1 mL of supernatant to 1.9 mL of 50 mM phosphate buffer in a cuvette (pH 7.0). We began the process by adding 1.0 mL of freshly produced 30 mM H 2 O 2 . Spectrophotometric analysis at 240 nm was used to determine the rate of H 2 O 2 breakdown. CAT activity was measured in µM/H 2 O 2 decomposition/minute [69].

Analysis of Acetylcholinesterase (AChE) Level
We quantitatively evaluated the acetylcholinesterase activity in the brain following the protocol. A total of 0.05 mL of supernatant, 3 mL of 0.01 M sodium phosphate buffer (pH 8), 0.10 mL of acetylthiocholine iodide and 0.10 mL of dithiothreitol n-butyrate (DTNB) were combined to create the test combination (Ellman reagent). The absorbance shift was immediately measured spectrophotometrically at 412 nm. Enzymatic activity in the supernatant was measured in M/mg protein units [70].

Analysis of Myeloperoxidase (MPO) Level
The samples of midbrain tissue were rapidly removed and frozen in liquid nitrogen. We measured out an appropriate amount of frozen tissue, washed it twice in phosphate buffer, pH 6.0 at 4-8 • C, and then homogenised it (IKA Homogenizer, Staufen, Germany) in a solution of 0.5% hexadecyltrimethylammonium bromide (HTAB) mixed in 50 mM potassium phosphate buffer (pH 6). The sample underwent centrifugation for 20 min at a temperature of 4 • C and 10,000 revolutions per minute. The samples were sonicated for 20 s, then frozen and thawed thrice. A 2.9 mL solution of 50 mM potassium phosphate buffer at pH 6 containing 0.167 mg/mL of O-dianisidine hydrochloride and 0.0005% H 2 O 2 was allowed to react with a 0.1 mL aliquot of the supernatant or standard (Sigma, Germany). After 5 min, the reaction was stopped with 0.1 cc of 1.2 M hydrochloric acid. The absorbance rate at 460 nm was measured using a spectrophotometer (Cecil 9000, Cambridge, UK). MPO activity was expressed as milli-units (mU) per gram weight of moist tissue [71].

Analysis of Reduced Glutathione (GSH) Level
After 1 mL of supernatant was precipitated with 1 mL of 4% sulfosalicylic acid, cold digestion took place at 4 • C for 1 h. The samples were centrifuged at 1200× g for 15 min. The supernatant was diluted to 1 mL with phosphate buffer (0.1 M, pH 8) and 5,5 -dithiobis-(2-nitrobenzoic acid) (DTNB). The newly produced yellow colour at 412 nm was instantly measured using a spectrophotometer. The GSH concentration in the supernatant was reported as mM per mg of protein [72].

Analysis of Nitrite Level
Colorimetric assays using the Greiss reagent (0.1% N-(1-naphthyl) ethylene diaminedihydrochloride, 1% sulfanilamide and 2.5% phosphoric acid) can measure nitric oxide concentrations. The absorbance at 540 nm is determined spectrophotometrically after mixing equal parts supernatant and Greiss reagent and incubating the mixture for 10 min at room temperature in the dark. The nitrite content of the supernatant was determined using a sodium nitrite standard curve and reported in M/mg protein units [73].

Analysis of Malondialdehyde (MDA) Level
Quantifying MDA in brain homogenates was performed by measuring the quantity of MDA at 532 nm following its reaction with thiobarbituric acid. Amounts of MDA were reported in nM/mg protein [26].

Analysis of Protein Carbonyl (PC) Level
An equal volume of 20% TCA was added to the tissue homogenate (0.25 mL). After adding 0.5 mL of 20% TCA, 0.25 mL of 10 mM 2,4-dinitrophenylhydrazine (DNPH) in 2.0 M HCl was added, and the mixture was left at room temperature for 1 h while being shaken every 10-15 min. We discarded the supernatant and washed the pellet three times with 1 mL of ethanol: ethyl acetate (1:1) to get rid of the free reagent. After letting the samples sit for 10 min, centrifugation was performed, and the resulting supernatant was thrown away. Within 15 min at 37-50 • C, the precipitated protein was redissolved in a guanidine hydrochloride solution and centrifuged at 11,000× g for 5 min to remove insoluble material. Spectrophotometric analysis of the carbonyl concentrations was performed at 370 nm (Shimadzu-1601, Nagoya, Japan). Using a molar extinction coefficient of 22 × 10 3 M −1 cm −1 , the data were represented as nM/mg protein [74].

Analysis of Total Glutathione Level
The total GSH level was measured in brain tissue homogenates with 20 volumes of icecold Tris-HCl buffer (20 mM, pH 7.4) containing 2 mM EDTA, followed by adding a similar volume of 10% trichloroacetic acid. After centrifugation (1000× g, 15 min), the supernatant was mixed with six volumes of ice-cold diethyl ether, followed by the evaporation of diethyl ether with N 2 gas. This step was repeated five times to remove trichloroacetic acid. The final samples were diluted with five volumes of ice-cold phosphate buffer (10 mM, pH 7.4). In the next step, 730 µL of 10 mM phosphate buffer (pH 7.4) containing 0.27 mM NADPH and 0.8 U GSH reductase was added to 20 µL of the diluted samples. After incubation for 5 min at 37 • C, 50 µL of 0.1 M phosphate buffer (pH 7.4) containing 4 mg/mL 5,5 -dithiobis (2-nitro) benzoic acid was added. The formation of p-nitrophenol was determined by measuring its absorbance at 412 nm for 6 min after adding 5,5 -dithiobis (2-nitro) benzoic acid [75].
Analysis of Hydrogen Peroxide (H 2 O 2 ) Level 50 mg of brain tissue fragments were homogenised with 2 mL potassium chloride (1.15%) solution. The tissue homogenate was diluted to a final volume of 10 µL before being combined with either 90 µL of physiologically buffered saline (pH 7.0) and 100 µL of horseradish peroxidase (1 U/mL) with 400 µL of homovanillic acids (HRP + HVA assay), or 90 µL of PBS and 100 µL of 1 U/mL horseradish peroxidase (HRP assay). After homogenisation, the samples were incubated at 37 • C for an hour. A total of 300 µL of phosphate-buffered saline (PBS) and 125 µL of 0.1 M glycine-NaOH buffer (pH 12.0) containing 25 mM EDTA (ethylenediaminetetraacetic acid) were added to each homogenate sample. An excitation wavelength of 312 nm and an emission wavelength of 420 nm were settled upon (Perkin Elmer Luminescence Spectrometer, Beaconsfield, UK) [76].

Histopathological Analysis of Striatum
Once the experimental schedule was completed, animals were anesthetised with sodium barbiturates at a 270 mg/kg dose, i.p., before decapitation. The midbrain was separated from the whole brain and cleaned with PBS. As directed, the sections were immersed in 4% paraformaldehyde in PBS at pH = 7.4. Tissue was embedded in paraffin wax and kept at 37 • C. A rotary microtome was used to section the paraffin wax blocks into 4-5 mm thicknesses. Morphological analysis was carried out with the help of staining with haematoxylin and eosin dye on the sections at 100× magnification. Neuronal cell density, the number of degenerating dopaminergic neurons and the formation of Lewy bodies were observed in the stained slide using a fluorescent microscope (MOTICAM-Ba310 image plus 2.0) [62].

Statistical Analysis
To compare the results of the various experimental groups, we used two-way ANOVA with post-hoc Bonferroni's test and one-way ANOVA repeated measures with post-hoc Tukey's multi-comparison test to evaluate the data generated. Multiple behavioural indicators were analysed using two-way ANOVA. However, one-way ANOVA was employed to examine the neurochemical parameters in brain homogenate, demyelination volume and TSTQ task. The statistical significance was set at 0.01. The sample size was determined to ensure that the data followed a normal distribution. The Windows version of GraphPad Prism 5.03 was used for the statistical analysis (GraphPad Software, San Diego, CA, USA). The mean and the standard deviation (SD) are the only statistics supplied.

Conclusions
In conclusion, we found that the intracerebroventricular injection of 6-OHDA causes unilateral lesions with PD-like behavioural, morphological, biochemical and neurochemical characteristics. After 8-21 days of treatment with FSK, the effects of 6-OHDA on behaviour, biochemistry and the nervous system are reversed, which leads to the control of CREBmediated signalling and the direct activation of D1 in the striatum. The inclusion of the test medicine FSK in the cAMP/PKA/CREB pathway, as well as its antioxidant, antiinflammatory and neuromodulatory actions, are all possibilities for the processes that could be responsible for the amelioration of PD-like neuropathological abnormalities. These results led researchers to speculate that FSK might be useful as a therapeutic component in the clinical management of PD. As a result, our study suggests and opens up the possibility for medicinal chemists to develop more effective and viable novel FSK analogues for treating neurological dysfunctions. ; resources, conceptualisation, supervision, S.K. and S.M. All date were generated in-house, and no paper mill was used. All authors agree to be accountable for all aspects of this work, ensuring integrity and accuracy. All authors have read and agreed to the published version of the manuscript.