Synthesis, Self-Assembly in Crystalline Phase and Anti-Tumor Activity of 2-(2-/4-Hydroxybenzylidene)thiazolo[3,2-a]pyrimidines

A series of new thiazolo[3,2-a]pyrimidines different by aryl substituents in 2 and 5 positions are synthesized and characterized in solution as well as in the crystalline phase using 1H and 13C NMR-, IR-spectroscopies, mass-spectrometry methods, and single crystal X-ray diffraction (SCXRD). The SCXRD study revealed the role of intermolecular H-bonding in the formation of supramolecular architectures (racemic monomers, centrosymmetric racematic dimers, or homochiral 1D chains) of obtained thiazolo[3,2-a]pyrimidines derivatives depending on solvents (aprotic DMSO or protic EtOH) used upon the crystallization process. Moreover, the in vitro study of cytotoxicity toward different tumor cells showed their high or moderate efficiency with moderate cytotoxicity against normal liver cells which allows to consider the obtained thiazolo[3,2-a]pyrimidine derivatives as promising candidates for application as antitumor agents.


Introduction
Heterocycles, especially those composed of different heterocyclic aromatic rings, generally possessing high biological activity represent organic compounds widely used for design of new antitumor drugs [1,2]. Among the various heterocyclic compounds in terms of antitumor activity, the thiazoles and pyrimidines derivatives are some of the most attractive precursors in medical chemistry due to their high pharmacological application in particular as anti-tuberculosis drugs, antioxidants, antimicrobial, antiviral agents, as medicaments for inflammatory disease treatment and antitumor therapy [3][4][5][6][7][8]. Nowadays, it is established that cancer of various types is a leading cause of morbidity and mortality worldwide, characterized by irregular cell growth. The number of deaths caused by cancer is still growing. According to the World Health Organization (WHO), in 2020, cancer accounts for more than 19 million new cases registered and ranks as one of the first leading cause of death in people under 70 years old [9]. For this purpose, in the past decades, the design of new heterocyclic compounds that demonstrate high antitumor activity both with low and normal cell cytotoxicity still attracts huge attention.
Recently, it was shown that due to high biological and pharmacological activities, heterocyclic compounds based on functional derivatives of 2-arylmethylidene thiazolo [3,2a]pyrimidines are appealing molecules demonstrating a high potential in anticancer drug design. In particular, it was demonstrated that indole derivative of triazolopyrimidine 1 (Figure 1) exceeded the cytotoxicity efficiency of the widely used antitumor drug doxorubicin by 1.15 times in the MCF-7 breast cancer cell line [10].
which practically corresponds to the one established for obatoclax drug [13]. Other similar compounds 4-6 containing thiazolo [3,2-a]pyrimidine fragment were found to be promising, and selective CDC25 phosphatase or casein kinase inhibitors play a key role in the regulation of the cell cycle observed in many types of cancer [14,15]. Earlier it was also reported that the triazolpyrimidine derivatives containing another molecular junction at C2 atom (dihydrazone derivatives) showed low cytotoxicity against normal and tumor cell lines [16]. Earlier, we reported on the synthesis and crystal structures of three derivatives of 2-(2-hydroxybenzylidene)thiazolo[3,2-a]pyrimidines containing phenyl-(7), 2-hydroxyphenyl-(8) and 4-methoxyphenyl- (9) substituents at the C5 carbon atom, where the conglomerate crystallization of one of the studied heterocycles (compound 8) based on thiazolo[3,2-a]pyrimidine (P212121 space group) into chiral 1D chains stabilized by intermolecular H-bonding is presented [17]. It should be mentioned that up to now no anti-tumor activity of the described compounds is studied. In addition, compound 1 has demonstrated high absorption ability in the gastrointestinal tract due to optimal hydrophilicity/lipophilicity of the molecule [11]. The related compound 2 ( Figure 1) has been used for the growth of Bcl-2 proteins inhibitor which leads to many types of tumor cells apoptosis [12]. Compound 3 ( Figure 1) has displayed remarkable inhibiting activity of the anti-apoptotic Bcl protein with IC 50 value of 3.4 µM, which practically corresponds to the one established for obatoclax drug [13]. Other similar compounds 4-6 containing thiazolo[3,2-a]pyrimidine fragment were found to be promising, and selective CDC25 phosphatase or casein kinase inhibitors play a key role in the regulation of the cell cycle observed in many types of cancer [14,15]. Earlier it was also reported that the triazolpyrimidine derivatives containing another molecular junction at C2 atom (dihydrazone derivatives) showed low cytotoxicity against normal and tumor cell lines [16].
Following this line, we synthesized three new compounds, 10-12, bearing 2hydroxybenzylidene substituent at the C2 carbon atom. In addition, in order to expand this series of new thiazolo [3,2-a]pyrimidines, the 4-hydroxybenzylidene derivatives were obtained: compound 13 (isomeric to compound 7), compound 14 (isomeric to compound 12), and compound 15 (isomeric to compound 10) (Figure 2). Herein the crystal structure of the obtained compounds 9-14 is discussed below. Moreover, the study of anti-tumor activity of a series of obtained 2-and 4-hydroxybenzylidene thiazolo [3,2-a]pyrimidine derivatives 7-15 is also presented.
The structures of synthesized compounds were studied using SCXRD, whose data are in agreement with the structure of these compounds in solution (for 1 H-, 13 C-NMR-, IR-, and mass-spectra see Figures S1-S19). In the crystalline phase, all the obtained compounds present similar structure when all carbon atoms, except C5, as well as hetero atoms (S and N) of bicyclic thiazolo[3,2-a]pyrimidines fragment are located in the same plane. The asymmetric sp 3 hybridized C5 atom is found to be disposed out of thiazolo[3,2a]pyrimidine plane displaying the distance of 0.126-0.273(1) Å and leading to a sofa
The structures of synthesized compounds were studied using SCXRD, whose data are in agreement with the structure of these compounds in solution (for 1 H-, 13 C-NMR-, IR-, and mass-spectra see Figures S1-S19). In the crystalline phase, all the obtained compounds present similar structure when all carbon atoms, except C5, as well as hetero atoms (S and N) of bicyclic thiazolo[3,2-a]pyrimidines fragment are located in the same plane. The asymmetric sp 3 hybridized C5 atom is found to be disposed out of thiazolo[3,2-a]pyrimidine plane displaying the distance of 0.126-0.273(1) Å and leading to a sofa conformation of six-membered pyrimidine ring (see Table S1). Interestingly, it was found out that for 12, C5 atom is found to be located in the same plane as thiazolo[3,2-a]pyrimidine bicyclic ring. All obtained molecules display only one C=C double bond configuration when 2-or 4hydroxybenzylidene fragment and S atom of thiazolyl moiety are cis-orientated (Z-isomers).
The aryl substituent connected to C5 atom occupies an axial position and is disposed in the plane bisecting N4-C5-C6 angle. For 11, the intramolecular O-π bonding is observed between the ortho OCH 3 group of phenyl substituent disposed at C5 atom and the π-system of pyrimidine moiety (d O17-C3N2centroid = 2.731 Å). Such type of interaction leading to stabilization of molecular conformation have been already reported recently [17,23].
Both the distances observed for C2-C9 and C9-C10 bonds as well as the dihedral angle formed between the phenol moiety of benzylidene substituent at C2 atom and thiazolo [3,2-a]pyrimidine bicyclic ring evidence the formation of a large electronconjugated system for all compounds (see Table S1). In the crystalline phase, all studied 2-hydroxybenzylidene)thiazolo[3,2-a]pyrimidines adopt conformation when 2-OH group is found to be syn orientated with carbonyl group of thiazolyl moiety.
For ester derivatives 8-10 and 12-14, the carbonyl group positioned at C6 atom is found to be conjugated with thiazolo[3,2-a]pyrimidine system which was attested by the values of the corresponding dihedral angle (see Table S2). In contrast, the ketoaryl derivative 11 displays relatively large dihedral angle with thiazolo[3,2-a]pyrimidine fragment equal to 39 • . Other characteristic bond distances and angles gathered in the Table S2 are found to be similar for all the studied compounds.
Due to presence of H-donor such as and H-acceptor atoms within the molecular structures, one may assume that the obtained thiazolo [3,2-a]pyrimidines are prone to be involved in supramolecular assembles formation in the crystalline phase as well as in solution. It is worth noting that the propensity of such type of molecules to participate in spontaneous self-assembly upon the H-bonding may play the important role in their medical application [24,25]. Indeed, as it was established, the supramolecular organization of obtained compounds in the crystalline phase depends on the mutual orientation of Hdonor/acceptor in the molecular scaffold as well as on the use of aprotic or protic solvent.
For 2-hydroxybenzylidene derivatives 9, 11, and 12, when crystallized from the ethanol solution, the formation of supramolecular racemic dimers displaying the centrosymmetric structure resulting from the intermolecular H-bonding between O11 atom of hydroxyl group and O3 atom belonging to carbonyl group of thiazolyl ring with the O . . . O distance range of 2.612(2)-2.797(2) Å was observed (see Figure 3, Table S3). tion when 2-or 4-hydroxybenzylidene fragment and S atom of thiazolyl moiety are cis-orientated (Z-isomers).
The aryl substituent connected to C5 atom occupies an axial position and is disposed in the plane bisecting N4-C5-C6 angle. For 11, the intramolecular O-π bonding is observed between the ortho OCH3 group of phenyl substituent disposed at C5 atom and the π-system of pyrimidine moiety (dO17-C3N2centroid = 2.731 Å). Such type of interaction leading to stabilization of molecular conformation have been already reported recently [17,23].
Both the distances observed for C2-C9 and C9-C10 bonds as well as the dihedral angle formed between the phenol moiety of benzylidene substituent at C2 atom and thiazolo[3,2-a]pyrimidine bicyclic ring evidence the formation of a large electron-conjugated system for all compounds (see Table S1). In the crystalline phase, all studied 2-hydroxybenzylidene)thiazolo[3,2-a]pyrimidines adopt conformation when 2-OH group is found to be syn orientated with carbonyl group of thiazolyl moiety.
For ester derivatives 8-10 and 12-14, the carbonyl group positioned at C6 atom is found to be conjugated with thiazolo[3,2-a]pyrimidine system which was attested by the values of the corresponding dihedral angle (see Table S2). In contrast, the ketoaryl derivative 11 displays relatively large dihedral angle with thiazolo[3,2-a]pyrimidine fragment equal to 39⁰. Other characteristic bond distances and angles gathered in the Table S2 are found to be similar for all the studied compounds.
Due to presence of H-donor such as and H-acceptor atoms within the molecular structures, one may assume that the obtained thiazolo [3,2-a]pyrimidines are prone to be involved in supramolecular assembles formation in the crystalline phase as well as in solution. It is worth noting that the propensity of such type of molecules to participate in spontaneous self-assembly upon the H-bonding may play the important role in their medical application [24,25]. Indeed, as it was established, the supramolecular organization of obtained compounds in the crystalline phase depends on the mutual orientation of Hdonor/acceptor in the molecular scaffold as well as on the use of aprotic or protic solvent.
For 2-hydroxybenzylidene derivatives 9, 11, and 12, when crystallized from the ethanol solution, the formation of supramolecular racemic dimers displaying the centrosymmetric structure resulting from the intermolecular H-bonding between O11 atom of hydroxyl group and O3 atom belonging to carbonyl group of thiazolyl ring with the O…O distance range of 2.612(2)-2.797(2) Å was observed (see Figure 3, Table S3).  Whereas the self-assembly of dimers based on 9 led to the formation of 2D supramolecular layer due to π-staking of thiazolo[3,2-a]pyrimidines moieties [17], and for 11, it was found out that ketophenyl C6 atom substituent displays significant π-bonding Whereas the self-assembly of dimers based on 9 led to the formation of 2D supramolecular layer due to π-staking of thiazolo[3,2-a]pyrimidines moieties [17], and for 11, it was found out that ketophenyl C6 atom substituent displays significant π-bonding (d C6centroid/C6centroid = 3.660 Å, dihedral angle equal to 0 • ) producing the chains (see Figure 4a). In addition, the obtained chains through interacting with each other through intermolecular pyrimidine-(2-hydroxy)phenyl π-bonding (d C3N2centroid/C6centroid = 3.670 Å, dihedral angle equal to 0 • ) also results in 2D supramolecular layers running along (022) crystallographic plane.
The changing of 2-or 4-metoxyphenyl substituent at C6 atom in 11 and 9 by 4bromophenyl fragment in 12 surprisingly led to the formation of 1D chain composed of H-bonded supramolecular dimers stacked through CH/π-bonding of C23 atom of ester group with bromophenyl moiety of adjacent molecules of 12 (d C23-C6centroid = 3.505 Å) (see Figure 4b).
The changing of 2-or 4-metoxyphenyl substituent at C6 atom in 11 and 9 by 4-bromophenyl fragment in 12 surprisingly led to the formation of 1D chain composed of Hbonded supramolecular dimers stacked through CH/π-bonding of C23 atom of ester group with bromophenyl moiety of adjacent molecules of 12 (dC23-C6centroid = 3.505 Å) (see Figure 4b).
In crystal, all supramolecular dimers 9, 11, and 12 demonstrate the parallel crystal packing. No solvent molecules were found in the unit cell of 9, 11, and 12.
Compounds 9 and 12 were also crystallized from DMSO solution. The SCXRD revealed the destroying of the H-bonded dimer assembly caused by the formation of solvate complexes 9-DMSO [17] and 12-DMSO in which solvent molecule was H-bonded via O30 atom with O11 atom of hydroxyl group of heterocyle (see Table S3, Figure 5). This is a remarkable example of the control on the self-assembly process using aprotic solvent displaying high H-accepting ability. In crystal of 12-DMSO, the solvate complexes are stacked into the chains due to weak π-π and Br/π bonding (see Figures S19). It should be noted that in 9-DMSO and 12-DMSO, molecules 9 and 12 adopt syn-orientation of carbonyl and hydroxyl groups and present flattened structure which has been already observed in the case of supramolecular H-bonded dimers 9, 11, and 12 (see Figure 3).  showing 2D layer and 1D chain formation resulting from both the intermolecular H-bonding and/or π-π/CH-π interactions (red and blue dotted lines, respectively). The ellipsoids are presented with 50% probability, H-atoms are omitted for clarity.
In crystal, all supramolecular dimers 9, 11, and 12 demonstrate the parallel crystal packing. No solvent molecules were found in the unit cell of 9, 11, and 12.
Compounds 9 and 12 were also crystallized from DMSO solution. The SCXRD revealed the destroying of the H-bonded dimer assembly caused by the formation of solvate complexes 9-DMSO [17] and 12-DMSO in which solvent molecule was H-bonded via O30 atom with O11 atom of hydroxyl group of heterocyle (see Table S3, Figure 5). This is a remarkable example of the control on the self-assembly process using aprotic solvent displaying high H-accepting ability. In crystal of 12-DMSO, the solvate complexes are stacked into the chains due to weak π-π and Br/π bonding (see Figure S19). It should be noted that in 9-DMSO and 12-DMSO, molecules 9 and 12 adopt syn-orientation of carbonyl and hydroxyl groups and present flattened structure which has been already observed in the case of supramolecular H-bonded dimers 9, 11, and 12 (see Figure 3). graphic plane.  showing 2D layer and 1D chain formation resulting from both the intermolecular H-bonding and/or π-π/CH-π interactions (red and blue dotted lines, respectively). The ellipsoids are presented with 50% probability, H-atoms are omitted for clarity.
The changing of 2-or 4-metoxyphenyl substituent at C6 atom in 11 and 9 by 4-bromophenyl fragment in 12 surprisingly led to the formation of 1D chain composed of Hbonded supramolecular dimers stacked through CH/π-bonding of C23 atom of ester group with bromophenyl moiety of adjacent molecules of 12 (dC23-C6centroid = 3.505 Å) (see Figure 4b).
In crystal, all supramolecular dimers 9, 11, and 12 demonstrate the parallel crystal packing. No solvent molecules were found in the unit cell of 9, 11, and 12.
Compounds 9 and 12 were also crystallized from DMSO solution. The SCXRD revealed the destroying of the H-bonded dimer assembly caused by the formation of solvate complexes 9-DMSO [17] and 12-DMSO in which solvent molecule was H-bonded via O30 atom with O11 atom of hydroxyl group of heterocyle (see Table S3, Figure 5). This is a remarkable example of the control on the self-assembly process using aprotic solvent displaying high H-accepting ability. In crystal of 12-DMSO, the solvate complexes are stacked into the chains due to weak π-π and Br/π bonding (see Figures S19). It should be noted that in 9-DMSO and 12-DMSO, molecules 9 and 12 adopt syn-orientation of carbonyl and hydroxyl groups and present flattened structure which has been already observed in the case of supramolecular H-bonded dimers 9, 11, and 12 (see Figure 3).  The use of methanol as a solvent upon the crystallization of compound 10 led to the generation of another solvate complex 10-MeOH presenting the formation of zigzag homochiral chains. The MeOH molecules in 10-MeOH forming the H-bonds with N8 atom of pyrimidine fragment and O11 atom of 2-hydroxybenzylidene moiety act as bridges connecting two adjacent thiazolo[3,2-a]pyrimidine molecules (see Table S3, Figure 6a). Within the chains, heterocyclic molecules display coplanar orientation. A similar connectivity pattern has been already observed earlier for 7-EtOH [17], but 10-MeOH displays a OON angle between the hydrogen bonds equal to 106.37(5) • , which is less compared to the one observed for 7-EtOH (157.90 • ). In crystal, the 1D chains are stacked parallelly forming 2D layers along (002) crystallographic plane due to π-π interaction between the thiazolyl groups (d C2-C3NScentroid = 3.494 Å, dihedral angle equal to 0 • ) and 2-hydroxybenzylidene moieties (d C9-C6centroid = 3.561 Å, dihedral angle equal to 0 • ) ( Figure 6b). 6a). Within the chains, heterocyclic molecules display coplanar orientation. A similar connectivity pattern has been already observed earlier for 7-EtOH [17], but 10-MeOH displays a OON angle between the hydrogen bonds equal to 106.37(5)°, which is less compared to the one observed for 7-EtOH (157.90°). In crystal, the 1D chains are stacked parallelly forming 2D layers along (002) crystallographic plane due to π-π interaction between the thiazolyl groups (dC2-C3NScentroid = 3.494 Å, dihedral angle equal to 0°) and 2-hydroxybenzylidene moieties (dC9-C6centroid = 3.561 Å, dihedral angle equal to 0°) (Figure 6b). In order to study the influence of hydroxyl group position within the structure of benzylidene substituent, the crystals of new 4-hydroxybenzylidenethiazolo[3,2-a]pyrimidines 13 and 14 were obtained by slow evaporation of their EtOH or DMSO solutions and analysis using SCXRD (see Experimental part). It was established that a self-assembly of 13 and 14 in the absence of strong H-acceptors results in 1D infinite zigzag homochiral chains formation involving the intermolecular H-bonding between O13 atom of 4-hydroxyl group acting as H-donor with.N8 atom of pyrimidine fragment behaving as H-acceptor (see Table S3, Figure 7). For 13, a weak chalcogen bonding between the O atom from hydroxyl group and S-atom from thiazolyl ring is also observed (dO13A-S1B = 3.301(6) Å).  In order to study the influence of hydroxyl group position within the structure of benzylidene substituent, the crystals of new 4-hydroxybenzylidenethiazolo[3,2-a]pyrimidines 13 and 14 were obtained by slow evaporation of their EtOH or DMSO solutions and analysis using SCXRD (see Experimental part). It was established that a self-assembly of 13 and 14 in the absence of strong H-acceptors results in 1D infinite zigzag homochiral chains formation involving the intermolecular H-bonding between O13 atom of 4-hydroxyl group acting as H-donor with.N8 atom of pyrimidine fragment behaving as H-acceptor (see Table S3, Figure 7). For 13, a weak chalcogen bonding between the O atom from hydroxyl group and S-atom from thiazolyl ring is also observed (d O13A-S1B = 3.301(6) Å).
pared to the one observed for 7-EtOH (157.90°). In crystal, the 1D chains are stacked parallelly forming 2D layers along (002) crystallographic plane due to π-π interaction between the thiazolyl groups (dC2-C3NScentroid = 3.494 Å, dihedral angle equal to 0°) and 2-hydroxybenzylidene moieties (dC9-C6centroid = 3.561 Å, dihedral angle equal to 0°) (Figure 6b). In order to study the influence of hydroxyl group position within the structure of benzylidene substituent, the crystals of new 4-hydroxybenzylidenethiazolo[3,2-a]pyrimidines 13 and 14 were obtained by slow evaporation of their EtOH or DMSO solutions and analysis using SCXRD (see Experimental part). It was established that a self-assembly of 13 and 14 in the absence of strong H-acceptors results in 1D infinite zigzag homochiral chains formation involving the intermolecular H-bonding between O13 atom of 4-hydroxyl group acting as H-donor with.N8 atom of pyrimidine fragment behaving as H-acceptor (see Table S3, Figure 7). For 13, a weak chalcogen bonding between the O atom from hydroxyl group and S-atom from thiazolyl ring is also observed (dO13A-S1B = 3.301(6) Å).  Although 13 and 14 demonstrate the similar supramolecular motif of zigzag chain and dihedral angle between adjacent heterocyclic molecules within the chain structure equal to 65 • , they are different by their crystal packing. In crystal of 13, the obtained chains are stacked without any specific intermolecular interactions. For 14, in contrast to 13, the formation of 3D supramolecular network is observed resulting from the π-stacking thiazolyl and 4-hydroxybenzylidene moieties of heterocyclic molecules belonging to neighboring chains (d C10-C3NS = 3.566 Å, dihedral angle equal to 0 • ) as well as the intermolecular O-π bonding involving O22 atom and thiazolyl moieties (d O22-C3NScentroid = 3.098 Å, ϕ = 142.27 • ) (see Figure 8 and Figure S20).
As for 9, 11, and 12, the use of DMSO as a solvent upon crystallization afforded the formation of solvate 14-DMSO in the crystalline phase where DMSO was found to be H-bonded with O11 atom belonging to hydroxyl group of 14. It is worth noting that involvement of DMSO in H-boding with heterocyclic compound led again to the destruction of the infinite polymeric chains (Figure 9) observed for 13 and 14 when crystallized from ethanol solution. As for 12-DMSO, 14-DMSO molecules are stacked in the crystalline phase into the chains resulting from the halogen-π bonding displaying a shorter distance between Br atom and thiazolyl group equal to d Br1-C2 = 3.226(1) Å (see Figure S21) with respect to 12-DMSO (see Figure S19).
Although 13 and 14 demonstrate the similar supramolecular motif of zigzag chain and dihedral angle between adjacent heterocyclic molecules within the chain structure equal to 65ᴼ, they are different by their crystal packing. In crystal of 13, the obtained chains are stacked without any specific intermolecular interactions. For 14, in contrast to 13, the formation of 3D supramolecular network is observed resulting from the π-stacking thiazolyl and 4-hydroxybenzylidene moieties of heterocyclic molecules belonging to neighboring chains (dC10-C3NS = 3.566 Å, dihedral angle equal to 0˚) as well as the intermolecular O-π bonding involving O22 atom and thiazolyl moieties (dO22-C3NScentroid = 3.098 Å, φ = 142.27˚) (see Figures 8 and S20). As for 9, 11, and 12, the use of DMSO as a solvent upon crystallization afforded the formation of solvate 14-DMSO in the crystalline phase where DMSO was found to be Hbonded with O11 atom belonging to hydroxyl group of 14. It is worth noting that involvement of DMSO in H-boding with heterocyclic compound led again to the destruction of the infinite polymeric chains (Figure 9) observed for 13 and 14 when crystallized from ethanol solution. As for 12-DMSO, 14-DMSO molecules are stacked in the crystalline phase into the chains resulting from the halogen-π bonding displaying a shorter distance between Br atom and thiazolyl group equal to dBr1-C2 = 3.226(1) Å (see Figure S21) with respect to 12-DMSO (see Figure S19). The most important stage in the investigation of new chemical compounds able to be used as potential drugs is the study of their cytotoxic properties. All prepared compounds  As for 9, 11, and 12, the use of DMSO as a solvent upon crystallization afforded the formation of solvate 14-DMSO in the crystalline phase where DMSO was found to be Hbonded with O11 atom belonging to hydroxyl group of 14. It is worth noting that involvement of DMSO in H-boding with heterocyclic compound led again to the destruction of the infinite polymeric chains ( Figure 9) observed for 13 and 14 when crystallized from ethanol solution. As for 12-DMSO, 14-DMSO molecules are stacked in the crystalline phase into the chains resulting from the halogen-π bonding displaying a shorter distance between Br atom and thiazolyl group equal to dBr1-C2 = 3.226(1) Å (see Figure S21) with respect to 12-DMSO (see Figure S19). The most important stage in the investigation of new chemical compounds able to be used as potential drugs is the study of their cytotoxic properties. All prepared compounds The most important stage in the investigation of new chemical compounds able to be used as potential drugs is the study of their cytotoxic properties. All prepared compounds were tested for their cytotoxicity against cancer and normal cell lines (Table 1). Data on cytotoxic activity are represented by IC 50 values (the concentration which causes the death of 50% of cells in the experimental population).
The tested compounds showed high and moderate activity against a number of cancer lines of various genesis and demonstrated moderate cytotoxicity against normal liver cells. The most significant results were shown in the case of cell lines of cervical carcinoma (M-HeLa) and human duodenal adenocarcinoma (HuTu80). Their cytotoxic effect was manifested at the level of the comparison drug sorafenib, and for 7, with respect to M-HeLa, it was found to be doubly increased compared to the known antitumor drug.
The selectivity of compounds against cancer cells is an important criterion for assessing the cytotoxic effect. For this purpose, the selectivity index (SI) was calculated as a ratio between the IC 50 value for normal cells and the IC 50 value for cancer cells. The selectivity index values for the tested compounds are shown in Table 2. It can be seen that the highest selectivity against the cancer lines M-HeLa and HuTu 80 was demonstrated by compound 7, whose SI value was 6.0 and 7.5, respectively. Generally, the compounds with SI ≥ 3 [26] are considered selective. According to these data, compound 7 can be considered selective toward the M-HeLa and HuTu 80 cell lines. It was worth noting that the reference drug sorafenib showed significantly lower selectivity with respect to 7. were tested for their cytotoxicity against cancer and normal cell lines (Table 1). Data on cytotoxic activity are represented by IC50 values (the concentration which causes the death of 50% of cells in the experimental population). The experiments were performed in triplicate. Results are expressed as the mean ± standard deviation The tested compounds showed high and moderate activity against a number of cancer lines of various genesis and demonstrated moderate cytotoxicity against normal liver cells. The most significant results were shown in the case of cell lines of cervical carcinoma (M-HeLa) and human duodenal adenocarcinoma (HuTu80). Their cytotoxic effect was manifested at the level of the comparison drug sorafenib, and for 7, with respect to M-HeLa, it was found to be doubly increased compared to the known antitumor drug.
The selectivity of compounds against cancer cells is an important criterion for assessing the cytotoxic effect. For this purpose, the selectivity index (SI) was calculated as a ratio between the IC50 value for normal cells and the IC50 value for cancer cells. The selectivity index values for the tested compounds are shown in Table 2. It can be seen that the highest selectivity against the cancer lines M-HeLa and HuTu 80 was demonstrated by compound 7, whose SI value was 6.0 and 7.5, respectively. Generally, the compounds with SI ≥ 3 [26] are considered selective. According to these data, compound 7 can be considered selective toward the M-HeLa and HuTu 80 cell lines. It was worth noting that the reference drug sorafenib showed significantly lower selectivity with respect to 7.
25.0 ± 1. 8 27.5 ± 2.  The experiments were performed in triplicate. Results are expressed as the mean ± standard deviation (SD); ns-no selectivity; Apoptosis is one of the most important mechanisms used for screening new anticancer agents. Apoptotic effects in M-HeLa cells were evaluated by flow cytofluorometry using annexin V kits that can bind phosphatidylethylserine. Phosphatidylserine is contained in minimal amounts on the membrane surface of healthy cells. Therefore, the interaction of annexin V with these cells is insignificant. During apoptosis, phosphatidylserine molecules can interact with the protein. This interaction leads to an increase in the fluorescence intensity of apoptotic cells which is detected by a flow cytofluorimeter. Apoptosis-inducing effect was evaluated using the leading compound 7 at concentrations of IC 50 /2 and IC 50 on the M-HeLa cell line (see Figure 10). After 24-h incubation in the presence of 7, dose-dependent apoptosis was observed in M-HeLa cells. Moreover, the observed effects were more active at the late stage of apoptosis.
using annexin V kits that can bind phosphatidylethylserine. Phosphatidylserine is contained in minimal amounts on the membrane surface of healthy cells. Therefore, the interaction of annexin V with these cells is insignificant. During apoptosis, phosphatidylserine molecules can interact with the protein. This interaction leads to an increase in the fluorescence intensity of apoptotic cells which is detected by a flow cytofluorimeter. Apoptosis-inducing effect was evaluated using the leading compound 7 at concentrations of IC50/2 and IC50 on the M-HeLa cell line (see Figure 10). After 24-h incubation in the presence of 7, dose-dependent apoptosis was observed in M-HeLa cells. Moreover, the observed effects were more active at the late stage of apoptosis. Evaluation of the mitochondria functions of the cell is another universal sign of apoptosis characteristic of eukaryotes [27]. With mitochondrial dysfunction, proapoptotic factors are released into the cytoplasm-cytochrome C, AIF, Smac/DIABLO, endonuclease G, as well as proforma caspases 2, 3, and 9-inducing cascade of caspases [28]. The Evaluation of the mitochondria functions of the cell is another universal sign of apoptosis characteristic of eukaryotes [27]. With mitochondrial dysfunction, proapoptotic factors are released into the cytoplasm-cytochrome C, AIF, Smac/DIABLO, endonuclease G, as well as proforma caspases 2, 3, and 9-inducing cascade of caspases [28]. The output of these protein factors can be realized both by breaking mitochondrial membranes and by activating specific channels in the outer membrane of mitochondria. The events described above are usually accompanied by a change in the permeability of the inner membrane of mitochondria for H + protons, which leads to a change in the membrane potential of mitochondria (∆Ψm). Methods for studying the membrane potential of mitochondria using flow cytofluorimetry are based on the use of cationic lipophilic dyes, which in the literature are called "mitochondrial probes". The principle of operation of these dyes is very simple-lipophilic probes spontaneously penetrate bilipid membranes (the surface membrane of the cell, as well as the outer and inner membranes of mitochondria) and accumulate in areas with a high pH value, that is, under the inner membrane of mitochondria. This effect results in a change of the intensity of cell fluorescence observed during the analysis on a flow cytofluorimeter. In this study, the fluorescent dye JC-10 from the Mitochondria Membrane Potential Kit was used to assess the change in ∆Ψm under the action of compound 7 at concentrations of IC 50 /2 and IC 50 on the M-HeLa cell line. JC-10 accumulates in the mitochondrial matrix and forms aggregates (J-aggregates) with red fluorescence in normal cells evidencing a high potential of the mitochondrial membrane. The mitochondrial membrane potential was found reduced in apoptotic cells which can be related with JC-10 starting to diffuse from the mitochondria and turning into a monomeric form (J-monomer) exhibiting green fluorescence. A decrease in the mitochondrial membrane potential of M-HeLa cells was observed after 24 h of treatment with the leading compound 7. The effect becomes more significant with an increase in the concentrations of the tested compounds up to IC 50 (see Figures 11 and 12). The obtained results confirm that the mechanism of cytotoxic action of 7 can be associated with the induction of apoptosis along the internal mitochondrial pathway.
An increase in the production of reactive oxygen species (ROS) by compounds also characterizes the development of apoptosis along the mitochondrial pathway. Mitochondria are both a potential source and target of ROS. An increase in ROS production leads to the disruption of mitochondrial functions and, subsequently, to irreversible damage to cells. In this regard, the effect of the leader compound 7 at IC 50 /2 and IC 50 concentrations on ROS production in M-HeLa cells was investigated using flow cytometry analysis and CellROX ® Deep Red flow cytometry kit. The data presented in Figure 13 show a significant increase in the fluorescence intensity of CellROX ® Deep Red compared to the control (unpainted cells). This indicates an increase in ROS production in the presence of the tested compound 7.
10 accumulates in the mitochondrial matrix and forms aggregates (J-aggregates) with red fluorescence in normal cells evidencing a high potential of the mitochondrial membrane. The mitochondrial membrane potential was found reduced in apoptotic cells which can be related with JC-10 starting to diffuse from the mitochondria and turning into a monomeric form (J-monomer) exhibiting green fluorescence. A decrease in the mitochondrial membrane potential of M-HeLa cells was observed after 24 h of treatment with the leading compound 7. The effect becomes more significant with an increase in the concentrations of the tested compounds up to IC50 (see Figures 11 and 12). The obtained results confirm that the mechanism of cytotoxic action of 7 can be associated with the induction of apoptosis along the internal mitochondrial pathway.   10 accumulates in the mitochondrial matrix and forms aggregates (J-aggregates) with red fluorescence in normal cells evidencing a high potential of the mitochondrial membrane. The mitochondrial membrane potential was found reduced in apoptotic cells which can be related with JC-10 starting to diffuse from the mitochondria and turning into a monomeric form (J-monomer) exhibiting green fluorescence. A decrease in the mitochondrial membrane potential of M-HeLa cells was observed after 24 h of treatment with the leading compound 7. The effect becomes more significant with an increase in the concentrations of the tested compounds up to IC50 (see Figures 11 and 12). The obtained results confirm that the mechanism of cytotoxic action of 7 can be associated with the induction of apoptosis along the internal mitochondrial pathway.   An increase in the production of reactive oxygen species (ROS) by compounds also characterizes the development of apoptosis along the mitochondrial pathway. Mitochondria are both a potential source and target of ROS. An increase in ROS production leads to the disruption of mitochondrial functions and, subsequently, to irreversible damage to cells. In this regard, the effect of the leader compound 7 at IC50/2 and IC50 concentrations on ROS production in M-HeLa cells was investigated using flow cytometry analysis and CellROX ® Deep Red flow cytometry kit. The data presented in Figure 13 show a significant increase in the fluorescence intensity of CellROX ® Deep Red compared to the control (unpainted cells). This indicates an increase in ROS production in the presence of the tested compound 7.  The effect of cytotoxic agents in general may be associated with a violation of the passage of eukaryotic cells phases of the cell cycle. This synchronizes and slows down the proliferation of a population of rapidly multiplying cells. The analysis of the cell cycle by quantifying the DNA content in the cell is a reliable research method that allows us to assess at which phase the cell cycle was stopped. This method allows us to study the phases of the cell cycle: the distribution of cells by G1/G0-, S-, and G2/M-phases of the cell cycle is estimated by determining the relative DNA content in cells using DNA-binding fluorescent dyes such as PI (propidium iodide), 7-AAD (7-aminoactinomycin), DAPI (4 6diamidino-2-phenylindole (DAPI)), Hoechst, SybrGreen, etc. Anomalies of the cell cycle detected on the histogram of DNA content frequencies are often observed after various types of cell damage, especially after exposing to chemical agents. In this work, cell cycle studies were carried out using the example of the leader compound 7 at concentrations of IC 50 /2 and IC 50 on the M-HeLa cell line using the fluorescent dye propidium iodide (PI). The fluorescence intensity of the stain cells correlated with the amount of DNA contained in them. The results of the cell cycle analysis after treatment with compound 7 for 24 h at concentrations of IC 50 /2 and IC 50 on the M-HeLa cell line showed a significant delay of cells in the G0/G1 phase compared with the reference sample( Figure 14). The processes of DNA damage and repair constantly occur in living cells. They can be caused by natural causes as a result of cellular respiration and metabolism or exposure to various chemical agents. Restoration of DNA damage is necessary for the normal functioning of cells and their preservation in a healthy state. Many proteins are involved in the processes of detecting and repairing DNA damage, among which groups of sensor proteins, mediators, converters, and effectors should be noted. Sensory proteins, such as Rad9, Rad1, and Hus1, accumulate at the site of DNA damage and provide phosphorylation of checkpoint proteins, which is influenced by intracellular kinases-ATM (ataxiatelangiectasia mutated) and ATR (ataxia-telangiectasia). Activation of mediator proteins such as H2AX, BRCA1, and SMC1, leads to stable protein-protein interaction, which facilitate the transmission of signals to intracellular kinases and at the same time activate checkpoint kinases. The kinases of the control points Chk1 and Chk2 are necessary to stop the cell cycle of the main control points, G1/S and G2/M, on which the integrity of the genome is checked. Vnutyuclear kinases ATR and ATM play an important role in the delay of the cell cycle in response to DNA damage. Intermediary proteins, such as Mre11 and MDC1, acquire post-translational modifications that are created by means of detector proteins. The intermediary proteins modified in this way then amplify the signal and The processes of DNA damage and repair constantly occur in living cells. They can be caused by natural causes as a result of cellular respiration and metabolism or exposure to various chemical agents. Restoration of DNA damage is necessary for the normal functioning of cells and their preservation in a healthy state. Many proteins are involved in the processes of detecting and repairing DNA damage, among which groups of sensor proteins, mediators, converters, and effectors should be noted. Sensory proteins, such as Rad9, Rad1, and Hus1, accumulate at the site of DNA damage and provide phosphorylation of checkpoint proteins, which is influenced by intracellular kinases-ATM (ataxia-telangiectasia mutated) and ATR (ataxia-telangiectasia). Activation of mediator proteins such as H2AX, BRCA1, and SMC1, leads to stable protein-protein interaction, which facilitate the transmission of signals to intracellular kinases and at the same time activate checkpoint kinases. The kinases of the control points Chk1 and Chk2 are necessary to stop the cell cycle of the main control points, G1/S and G2/M, on which the integrity of the genome is checked. Vnutyuclear kinases ATR and ATM play an important role in the delay of the cell cycle in response to DNA damage. Intermediary proteins, such as Mre11 and MDC1, acquire post-translational modifications that are created by means of detector proteins. The intermediary proteins modified in this way then amplify the signal and transmit it to effector proteins such as Chk2, MDM2, and p53. The tumor suppressor protein p53 plays a crucial role in the cell cycle arrest or apoptosis after various stresses, including chemical damage to DNA. The main target of p53 is p21 (a cyclin-dependent kinase inhibitor), which causes the cell cycle to stop at the G1/G0 stage. The coordinated functioning of the protein groups described above and some others causes the cell cycle to stop, which makes possible to control DNA damage [29].
Classical methods of assessing genotoxicity reveal mutations associated with increased or loss of function, but are not informative enough in studying the molecular mechanisms of DNA damage. The MILLIPLEX ® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead kit allows to detect the expression and phosphorylation of a number of proteins involved in DNA damage, providing a faster and more accurate assessment of the state of the cell after exposure to genotoxic compounds. The 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit is used to detect changes in phosphorylated Chk1 (Ser345), Chk2 (Thr68), H2AX (Ser139) and p53 (Ser15), as well as total protein levels of ATR, MDM2, and p21 in cellular lysates using the Luminex ® system. Figure 15 shows the data of multiplex analysis performed using the 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit. The obtained results characterize the effect of the leading compound 7 on the DNA of M-HeLa cells at concentrations of IC 50 /2 and IC 50 . It is shown that the values of the average fluorescence intensity of the markers Chk1, Chk2, H2AX, MDM2, p53, and p21 significantly increase with increasing concentration compared to the control untreated compound 7 samples. The fluorescence intensity of OTR markers also increases relative to the reference sample. A high concentration of p21 protein may indicate the arrest of the M-HeLa cell cycle in the G1/G0 phase. This assumption was confirmed by cell cycle analysis using the fluorescent dye propidium iodide. Thus, the cytotoxic effect of the tested compounds can be characterized by the induction of apoptosis along the internal pathway associated with mitochondrial dysfunction, cell cycle delay in the G1/G0 phase. Classical methods of assessing genotoxicity reveal mutations associated with increased or loss of function, but are not informative enough in studying the molecular mechanisms of DNA damage. The MILLIPLEX ® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead kit allows to detect the expression and phosphorylation of a number of proteins involved in DNA damage, providing a faster and more accurate assessment of the state of the cell after exposure to genotoxic compounds. The 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit is used to detect changes in phosphorylated Chk1 (Ser345), Chk2 (Thr68), H2AX (Ser139) and p53 (Ser15), as well as total protein levels of ATR, MDM2, and p21 in cellular lysates using the Luminex ® system. Figure 15 shows the data of multiplex analysis performed using the 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit. The obtained results characterize the effect of the leading compound 7 on the DNA of M-HeLa cells at concentrations of IC50/2 and IC50. It is shown that the values of the average fluorescence intensity of the markers Chk1, Chk2, H2AX, MDM2, p53, and p21 significantly increase with increasing concentration compared to the control untreated compound 7 samples. The fluorescence intensity of OTR markers also increases relative to the reference sample. A high concentration of p21 protein may indicate the arrest of the M-HeLa cell cycle in the G1/G0 phase. This assumption was confirmed by cell cycle analysis using the fluorescent dye propidium iodide. Thus, the cytotoxic effect of the tested compounds can be characterized by the induction of apoptosis along the internal pathway associated with mitochondrial dysfunction, cell cycle delay in the G1/G0 phase.
NMR experiments were performed on Bruker Avance instruments with an operating frequency of 400, 500, and 600 MHz for shooting 1 H and 13 C NMR spectra. Chemical shifts were determined relative to the signals of residual protons of the CDCl 3 or DMSOd 6 solvents.
IR spectra in KBr tablets were recorded on a Bruker Vector-22. Electrospray ionization (ESI) mass spectra were obtained using a Bruker AmaZon X ion trap mass spectrometer. Melting points were determined on a BOETIUS melting table with an RNMK 05 imaging device.

General Method for Compounds 10-15 Preparation
The hydrochloride of the appropriate thiazolo[3,2-a]pyrimidine (1 mol) was mixed with CHCl 3 (50 mL) and water (50 mL) solution containing 1 mol NaOH and stirred for 30 min at room temperature. Then, aromatic aldehyde (2-or 4-hydroxybenzaldehyde) (1 mol) and a catalytic amount (several drops) of pyrrolidine were added. A resulting mixture was stirred for 6 h under refluxing conditions. After cooling, the formed precipitate was filtered off, washed with ethanol, and purified by recrystallization from methanol followed by drying in vacuum for 2 h at 100 • C temperature affording a pure product. Ethyl

Crystallization Conditions
Crystals of 11, 12, 13, and 14 suitable for X-ray diffraction study were obtained by slow evaporation of ethanol solution (25 mL) containing 0.02 mol of dissolved compound after 5 days.
Crystals of solvates 12-DMSO and 14-DMSO suitable for X-ray diffraction study were obtained by slow evaporation of DMSO solution (10 mL) containing 0.02 mol of dissolved compound after 7 days.
Crystals of solvate 10-MeOH suitable for X-ray diffraction study were obtained by slow evaporation of MeOH solution (15 mL) containing 0.02 mol of the dissolved compound after 3 days.
X-ray diffraction analysis of 10-MeOH, 11, 12-DMSO, 13, 14, and 14-DMSO was performed on a Bruker D8 QUEST automatic three-circle diffractometer with a PHOTON III twodimensional detector and an IµS DIAMOND microfocus X-ray tube (λ[Mo Kα] = 0.71073 Å) at cooling conditions. Data collection and processing of diffraction data were performed using APEX3 software package.
All structures were solved by the direct method using the SHELXT program [33] and refined by the full-matrix least squares method over F 2 using the SHELXL program [34]. All calculations were performed in the WinGX software package [35], the calculation of the geometry of molecules and intermolecular interactions in crystals was carried out using the PLATON program [36], the drawings of molecules were performed using the ORTEP-3 [35] and MERCURY [37] programs.
Non-hydrogen atoms were refined in the anisotropic approximation. The positions of the hydrogen atoms H(O) were determined using difference Fourier maps, and these atoms were refined isotropically. The remaining hydrogen atoms are placed in geometrically calculated positions and included in the refinement in the "riding" model. The crystal of compound 9, 12, and 14 is a solvate with DMSO (1:1); crystal of compound 9-solvate with methanol (1:1). Crystallographic data of structures 9-14 were deposited at the Cambridge Crystallographic Data Center, registration numbers and the most important characteristics are given in Table 3.

MTT Assay
The cytotoxic effect on cells was determined using the colorimetric method of cell proliferation-the MTT test. NADP-H-dependent cellular oxidoreductase enzymes can, under certain conditions, reflect the number of viable cells. These enzymes are able to reduce the tetrazolium dye (MTT)-3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide to insoluble blue-violet formazan, which crystallizes inside the cell. The amount of formazan formed is proportional to the number of cells with active metabolism. Cells were seeded on a 96-well Nunc plate at a concentration of 5 × 10 3 cells per well in a volume of 100 µL of medium and cultured in a CO 2 incubator at 37 • C until a monolayer was formed. Then the nutrient medium was removed and 100 µL of solution of the test drug in the given dilutions was added to the wells, which were prepared directly in the nutrient medium with the addition of 5% DMSO to improve solubility. After 48 h of incubation of the cells with the tested compounds, the nutrient medium was removed from the plates and 100 µL of the nutrient medium without serum with MTT at a concentration of 0.5 mg/mL was added and incubated for 4 h at 37 • C. Formazan crystals were added to 100 µL of DMSO. Optical density was recorded at 540 nm on an Invitrologic microplate reader (Novosibirsk, Russia). The experiments for all compounds were repeated three times.

Induction of Apoptotic Effects by Test Compounds Flow Cytometry Assay
Cell Culture. M-HeLa cells at 1 × 10 6 cells/well in a final volume of 2 mL were seeded into six-well plates. After 48 h of incubation, various concentrations of compound 7 were added to the wells.
Cell Apoptosis Analysis. The cells were harvested at 2000 rpm for 5 min and then washed twice with ice-cold PBS, followed by resuspension in binding buffer. Next, the samples were incubated with 5 µL of annexin V-Alexa Fluor 647 (Sigma-Aldrich, St. Louis, MO, USA) and 5 µL of propidium iodide for 15 min at room temperature in the dark. Finally, the cells were analyzed by flow cytometry (Guava easy Cyte, MERCK, Kenilworth, NJ, USA) within 1 h. The experiments were repeated three times.
Mitochondrial Membrane Potential. Cells were harvested at 2000 rpm for 5 min and then washed twice with ice-cold PBS, followed by resuspension in JC-10 (10 µg/mL) and incubation at 37 • C for 10 min. After the cells were rinsed three times and suspended in PBS, the JC-10 fluorescence was observed by flow cytometry (Guava easy Cyte, MERCK, Kenilworth, NJ, USA).

Detection of Intracellular ROS
M-HeLa cells were incubated with compound 7 at concentrations of IC 50 /2 and IC 50 for 48 h. ROS generation was investigated using flow cytometry assay and CellROX ® Deep Red flow cytometry kit. For this, M-HeLa cells were harvested at 2000 rpm for 5 min and then washed twice with ice-cold PBS, followed by resuspension in 0.1 mL of medium without FBS, to which 0.2 µL of CellROX ® Deep Red was added and incubated at 37 • C for 30 min. After three times washing the cells and suspending them in PBS, the production of ROS in the cells was immediately monitored using flow cytometer (Guava easy Cyte, MERCK, Kenilworth, NJ, USA).

Multiplex Analysis of Markers DNA Damage/Genotoxicity
The studies were carried out according to the standard protocol. M-HeLa cells were incubated for 24 h with the test substance. Cells were lysed in MILLIPLEX ® MAP Lysis buffer containing protease inhibitors. A total of 20 µg of total protein of each lysate diluted in MILLIPLEX ® MAP Assay Buffer 2 was analyzed according to the analysis protocol (the lysate was incubated at 4 • C overnight). The mean fluorescence intensity (MFI) was detected using Luminex ® system, MERCK, Kenilworth, NJ, USA.

Statistical Analysis
The IC 50 values were calculated using the online calculator MLA-Quest Graph™ IC 50 Calculator AAT Bioquest, Inc., 14 February 2021. Statistical analysis was performed using the Mann-Whitney test (p < 0.05). Tabular and graphical data contain the averages and standard error.

Conclusions
Synthesis of new 2-(2-and 4-hydroxybenzylidene)thiazolo[3,2-a]pyrimidine derivatives 10-15 containing phenyl, m-nitrophenyl, p-bromophenyl, and o-anisyl substituents at the C5 atom was successfully achieved in high yields. The single crystal X-ray diffraction study revealed that the supramolecular motif in the crystalline phase of the obtained compounds can be controlled by adjusting the interplay between various types of noncovalent interactions such as H-, Br-π, O-π, π-π bonding through the rational choice of the substituents at C2, C5, and C6 atoms. Depending on the used 2-or 4-hydroxybenzylidene derivatives, the self-assembly pathway leads to the formation of racemic H-bonded dimers (11,12) or infinite 1D homochiral chains (13, 14), respectively, which can be explained by the close disposition of H-bond donor and H-bond acceptor observed for 11 and 12, and relatively high H-accepting ability of N8 pyrimidine atom in the case of 13 and 14. Moreover, the 1D chains can be generated for 2-hydroxybenzylidene by involving protic EtOH or MeOH acting as bridges in intermolecular H-bonding between the obtained heterocycles (10-MeOH). It was demonstrated that upon crystal packing, the relatively weak Br-π, O-π, and π-π interactions also play a significant role which can be enhanced by switching off the intermolecular H-bonding when DMSO molecules are involved in coordination with OH-donor group.
A series composed of new compounds 2-(2-and 4-hydroxybenzylidene)thiazolo[3,2a]pyrimidine derivatives 10-15 and their earlier reported analogues 7-9 were tested in vitro as antitumor agents. The studied compounds showed high or moderate activity against a number of cancer lines of various genesis and demonstrated moderate cytotoxicity against normal liver cells. The most significant results of the studied compounds were shown in relation to the cell lines of cervical carcinoma (M-HeLa) and human duodenal adenocarcinoma (HuTu 80). Their cytotoxic effect was confirmed by comparing with drug sorafenib, and compound 7 has demonstrated two times higher efficiency. According to the calculated indices of selectivity of cytotoxic action, compound 7 also demonstrates better selectivity with respect to the M-HeLa and HuTu 80cell lines. Using the flow cytometry method and multiplex analysis of DNA damage/genotoxicity markers, it was shown that the cytotoxic effect of the tested compound 7 can be characterized by the induction of apoptosis along the internal pathway associated with mitochondrial dysfunction, cell cycle delay in the G1/G0 phase. The design of new thiazolo[3,2-a]pyrimidines exhibiting higher anti-tumor activity with simultaneously low normal cells toxicity is in progress.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are contained within the article or in Supplementary Materials, or are available on request from the corresponding author Igor Antipin.