A Two-Stage Culture Strategy for Scenedesmus sp. FSP3 for CO2 Fixation and the Simultaneous Production of Lutein under Light and Salt Stress

In this study, Scenedesmus sp. FSP3 was cultured using a two-stage culture strategy for CO2 fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO2 introduced to promote the rapid growth and CO2 fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L−1 and a light intensity of 160 μmol m−2 s−1 were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO2 fixation were simultaneously achieved. The biomass productivity and carbon fixation rate of Scenedesmus sp. FSP3 reached 0.58 g L−1 d−1 and 1.09 g L−1 d−1, with a lutein content and yield as high as 6.45 mg g−1 and 2.30 mg L−1 d−1, respectively. The results reveal a commercially feasible way to integrate microalgal lutein production with CO2 fixation processes.


Introduction
Since the 21st century, global carbon emissions have accelerated sharply, with global CO 2 emissions increasing by 40% from 2000-2019. In addition, global CO 2 emissions rebounded in 2021, reaching their highest-ever annual level of 36.3 Gt, according to the International Energy Agency (IEA) [1]. The level of atmospheric CO 2 concentration reached 419 ppm in May 2021, which is the highest on record and continues to increase. It will further cause serious environmental problems, such as the greenhouse effect, global warming, ocean acidification, etc. affecting the sustainable development of human society and people's life and health. CO 2 fixation based on microalgae is considered one of the most promising and sustainable carbon capture, utilization, and storage (CCUS) technologies [2]. Microalgae are microorganisms with chloroplasts that are capable of photosynthesis. Marine phytoplankton represented by microalgae are responsible for 40% of global CO 2 fixation each year. Microalgae have a larger specific surface area and are more efficient at chloroplast photosynthesis compared to other higher plant cells. Moreover, their own carbon concentration mechanism (CCM), meaning their carbon sequestration rate, is much higher than that of terrestrial plants, which has great potential for development [3].
Microalgae can efficiently use light energy, CO 2 , and water for photosynthesis to produce oxygen and synthesize carbohydrates that store energy. Through further biochemical

Growth and CO 2 Fixation Rates of Microalgae under Different CO 2 Concentrations
In general, CO 2 concentrations below 5% are suitable for microalgal growth [9], while higher CO 2 concentrations are considered harmful to microalgal growth. Our results show that Scenedesmus sp. FSP3 had a high tolerance to CO 2 , which can grow well under 30% CO 2 ( Figure 1A). As is shown in Figure 1A, Scenedesmus sp. FSP3 showed higher biomass concentration, biomass productivity, and CO 2 fixation rate under CO 2 concentrations ranging from 5 to 30% when compared to that under 0.03%. Notably, the growth of Scenedesmus sp. FSP3 greatly enhanced at a CO 2 concentration of 5%, reaching a maximum biomass concentration of 2.99 g L −1 after seven days of cultivation ( Figure 1A). The maximum biomass productivity and CO 2 fixation rate of Scenedesmus sp. FSP3, under a CO 2 concentration of 5%, was 0.41 g L −1 d −1 and 0.77 g L −1 d −1 (Figure 1B), respectively. Therefore, a 5% CO 2 concentration was selected for further study.

Addition of CO 2 Solubilization Enhancer for Better Carbon Absorption
The solubility of CO 2 in the culture solution has a great influence on the growth of microalgae [10]. However, the solubility of CO 2 in water is only 1.45 g L −1 at 25 • C and 1 atm, so when CO 2 is introduced into the culture medium, the short residence time and slow diffusion of CO 2 in the culture causes most of the CO 2 to escape [11], which limits the carbon sequestration of the microalgae in the medium. The addition of a CO 2 solubilization enhancer was reported to be effective in promoting the solubility of CO 2 and carbon sequestration of microalgae [10][11][12].

Addition of CO2 Solubilization Enhancer for Better Carbon Absorption
The solubility of CO2 in the culture solution has a great influence on the growth of microalgae [10]. However, the solubility of CO2 in water is only 1.45 g L −1 at 25 °C and 1 atm, so when CO2 is introduced into the culture medium, the short residence time and slow diffusion of CO2 in the culture causes most of the CO2 to escape [11], which limits the carbon sequestration of the microalgae in the medium. The addition of a CO2 solubilization enhancer was reported to be effective in promoting the solubility of CO2 and carbon sequestration of microalgae [10][11][12].
In our study, a 5% CO2 concentration was used for the cultivation, and methanol, polyethylene glycol (PEG200), propylene carbonate (PC), polyethylene glycol dimethyl ether (NHD), and N-methylpyrrolidone (NMP) were selected as the CO2 solubilization enhancers. They were reported to be harmless to microalgae within the range of concentrations used in our present study [11], except for PEG200, which is not metabolized by microalgae.
As is shown in Figure 2A, after seven days of cultivation, the biomass concentrations were 2.25, 2.32, 2.46, 2.55, and 2.49 g L −1 in those cultures supplemented with 5 mmol L −1 methanol, NHD, NMP, PC, and PEG200, respectively. In comparison, the biomass concentration obtained in the blank was 2.12 g L −1 . The growth of the microalgae was promoted to varying degrees under all conditions, while the addition of PC achieved the most obvious growth promotion effect, with its biomass concentration increased by 20.28% when compared to the blank. In our study, a 5% CO 2 concentration was used for the cultivation, and methanol, polyethylene glycol (PEG200), propylene carbonate (PC), polyethylene glycol dimethyl ether (NHD), and N-methylpyrrolidone (NMP) were selected as the CO 2 solubilization enhancers. They were reported to be harmless to microalgae within the range of concentrations used in our present study [11], except for PEG200, which is not metabolized by microalgae.
As is shown in Figure 2A, after seven days of cultivation, the biomass concentrations were 2.25, 2.32, 2.46, 2.55, and 2.49 g L −1 in those cultures supplemented with 5 mmol L −1 methanol, NHD, NMP, PC, and PEG200, respectively. In comparison, the biomass concentration obtained in the blank was 2.12 g L −1 . The growth of the microalgae was promoted to varying degrees under all conditions, while the addition of PC achieved the most obvious growth promotion effect, with its biomass concentration increased by 20.28% when compared to the blank.  The effect of PC on CO2 absorption was further investigated, and the results suggested that the growth and CO2 absorption significantly improved under the addition of PC compared with the blank. As is shown in Figure 2B, the concentration of CO2 in the medium (Input) with PC added or as a blank was basically constant, with an average concentration of 4.49%. In general, the CO2 absorption rate of the medium with PC and the blank was 18.98% and 17.14%, respectively. The effluent CO2 concentration of the medium  The effect of PC on CO 2 absorption was further investigated, and the results suggested that the growth and CO 2 absorption significantly improved under the addition of PC compared with the blank. As is shown in Figure 2B, the concentration of CO 2 in the medium (Input) with PC added or as a blank was basically constant, with an average concentration of 4.49%. In general, the CO 2 absorption rate of the medium with PC and the blank was 18.98% and 17.14%, respectively. The effluent CO 2 concentration of the medium with the PC added (PC output) and the effluent CO 2 concentration of the blank (Blank output) showed little difference during the first two days; as the biomass concentration gap between the PC added and the blank increased in the subsequent cultures, the PC output showed a lower CO 2 concentration ( Figure 2B). The culture medium with added PC absorbed more CO 2 and had a lower CO 2 concentration output. On the fourth day, the difference in the CO 2 absorption rate for the PC added and the blank reached a maximum of 4.69%, along with the largest biomass concentration difference ( Figure 2B). Maximum biomass productivity reached 0.55 g L −1 d −1 ; meanwhile the CO 2 fixation rate reached 1.03 g L −1 d −1 under a 5% CO 2 concentration with added PC.

Two-Stage Strategy Promoting Lutein Accumulation under Light and Salt Stress
Scenedesmus sp. FSP3 were cultured using a two-stage culture strategy. In the first stage, Scenedesmus sp. FSP3 were cultivated under a light intensity of 90 µmol m −2 s −1 and 5% CO 2 infusion to facilitate cell growth and CO 2 fixation. Furthermore, in the second stage, the microalgae were cultivated under stress to stimulate lutein accumulation. In previous reports, the lutein levels were influenced by temperature, light intensity, salt concentration, nitrogen source availability, oxidation, and other factors [13]. According to the preliminary experiment, we investigated the effects of light stress and salt stress on the lutein content of Scenedesmus sp. FSP3 in the second stage culture.

Effect of Light Intensity on Lutein Content
In the present study, different light intensities of 30, 90, 150, and 210 µmol m −2 s −1 were set to investigate the growth and dynamics of the lutein content of Scenedesmus sp. FSP3. As is shown in Figure 3A, Scenedesmus sp. FSP3 showed little difference in growth under these four light intensities, and the highest biomass concentration (2.75 g L −1 d −1 ) was obtained at 90 µmol m −2 s −1 ( Figure 3A).
The lutein content under a light intensity of 30 µmol m −2 s −1 was the highest on the eighth day, reaching 5.44 mg g −1 . Under a 90 and 210 µmol m −2 s −1 light intensity, Scenedesmus sp. FSP3 showed a low accumulation of lutein content, and the peak lutein content was achieved on day four, at 150 µmol m −2 s −1 with 5.54 mg g −1 in our study ( Figure 3B).

Effect of Salt Concentration on Lutein Content
According to the preliminary experiment, NaCl concentration gradients of 0, 50, 150, and 200 mmol L −1 were set to compare the dynamic changes in the growth and lutein content of Scenedesmus sp. FSP3 at different NaCl concentrations. As is shown in Figure 3C, Scenedesmus sp. FSP3 grew best in the medium with a 0 mmol L −1 NaCl concentration, while the growth of Scenedesmus sp. FSP3 was inhibited in the medium with a 50-200 mmol L −1 NaCl concentration, with little difference.
During the period from four to eight days, the lutein content of those microalgae under a 0-150 mmol L −1 salt concentration increased with the time of stress, reaching a maximum of 5.51 mg g −1 under a 50 mmol L −1 NaCl concentration on day 8 ( Figure 3D). On the eighth day, the lowest lutein content of Scenedesmus sp. FSP3 was found under a 200 mmol L −1 NaCl concentration. This is probably because the high salt concentration caused damage to the microalga cells, resulting in a reduction in lutein content. Therefore, the appropriate NaCl concentration is also crucial for the accumulation of lutein in microalgae. under these four light intensities, and the highest biomass concentration (2.75 g L −1 d −1 ) was obtained at 90 μmol m −2 s −1 ( Figure 3A). The lutein content under a light intensity of 30 μmol m −2 s −1 was the highest on the eighth day, reaching 5.44 mg g −1 . Under a 90 and 210 μmol m −2 s −1 light intensity, Scenedesmus sp. FSP3 showed a low accumulation of lutein content, and the peak lutein content was achieved on day four, at 150 μmol m −2 s −1 with 5.54 mg g −1 in our study ( Figure 3B).

Effect of Salt Concentration on Lutein Content
According to the preliminary experiment, NaCl concentration gradients of 0, 50, 150, and 200 mmol L −1 were set to compare the dynamic changes in the growth and lutein content of Scenedesmus sp. FSP3 at different NaCl concentrations. As is shown in Figure  3C, Scenedesmus sp. FSP3 grew best in the medium with a 0 mmol L −1 NaCl concentration, while the growth of Scenedesmus sp. FSP3 was inhibited in the medium with a 50-200 mmol L −1 NaCl concentration, with little difference.
During the period from four to eight days, the lutein content of those microalgae under a 0-150 mmol L −1 salt concentration increased with the time of stress, reaching a maximum of 5.51 mg g −1 under a 50 mmol L −1 NaCl concentration on day 8 ( Figure 3D). On the eighth day, the lowest lutein content of Scenedesmus sp. FSP3 was found under a 200 mmol L −1 NaCl concentration. This is probably because the high salt concentration caused damage to the microalga cells, resulting in a reduction in lutein content. Therefore,

Response Surface Methodology for Lutein Production
In the above experiments, Scenedesmus sp. FSP3 lutein content was indeed enhanced under light intensity stress and salt stress, but the extent of the enhancement was limited; the maximum lutein content obtained in the second stage of culturing under light and salt stress was 5.54 and 5.51 mg g −1 ( Figure 3B,D), respectively. Therefore, it is necessary to optimize light stress and salt stress simultaneously, further stimulating Scenedesmus sp. FSP3 in order to accumulate lutein. Cultivation time also had an important effect on lutein, according to Figure 3D. The light intensity, NaCl concentration, and cultivation time were optimized by response surface methodology (RSM). The experimental design of the response surface methodology and experimental results are shown in Table S1. The response surface ( Figure 4) and contour map ( Figure S1) for lutein content were obtained by software analysis. According to this model, the maximum lutein content was obtained when the cultivation time, light intensity, and NaCl concentration were 4.5 d, 160 µmol m −2 s −1 , and 156.6 mmol L −1 , respectively. Then, Scenedesmus sp. FSP3 was cultivated under these optimized conditions and the results of the growth and lutein content of Scenedesmus sp. FSP3 is shown in Figure 5.
The initial seeding density was 0.22 g L −1 ; after three days of culture, the biomass concentration reached 1.97 g L −1 , and the maximum biomass productivity was 0.58 g L −1 d −1 ( Figure 5). Then, we transferred the microalga cells to the second stage, where the growth of Scenedesmus sp. FSP3 was delayed for the following two days, then grew slowly. The lutein content of Scenedesmus sp. FSP3 was 3.17 mg g −1 at the beginning of the second stage but then gradually accumulated and reached a maximum of 6.45 mg g −1 after 7.5 days ( Figure 5) under combined light and salt stress. The lutein productivity achieved was 2.30 mg L −1 d −1 . By using this culture method, high lutein production and CO 2 fixation were simultaneously achieved.

Response Surface Methodology for Lutein Production
In the above experiments, Scenedesmus sp. FSP3 lutein content was indeed enhanced under light intensity stress and salt stress, but the extent of the enhancement was limited; the maximum lutein content obtained in the second stage of culturing under light and salt stress was 5.54 and 5.51 mg g −1 (Figures 3B,D), respectively. Therefore, it is necessary to optimize light stress and salt stress simultaneously, further stimulating Scenedesmus sp. FSP3 in order to accumulate lutein. Cultivation time also had an important effect on lutein, according to Figure 3D. The light intensity, NaCl concentration, and cultivation time were optimized by response surface methodology (RSM). The experimental design of the response surface methodology and experimental results are shown in Table S1. The response surface ( Figure 4) and contour map ( Figure S1) for lutein content were obtained by software analysis. According to this model, the maximum lutein content was obtained when the cultivation time, light intensity, and NaCl concentration were 4.5 d, 160 μmol m −2 s −1 , and 156.6 mmol L −1 , respectively. Then, Scenedesmus sp. FSP3 was cultivated under these optimized conditions and the results of the growth and lutein content of Scenedesmus sp. FSP3 is shown in Figure 5. The initial seeding density was 0.22 g L −1 ; after three days of culture, the biomass concentration reached 1.97 g L −1 , and the maximum biomass productivity was 0.58 g L −1 d −1 (Figure 5). Then, we transferred the microalga cells to the second stage, where the growth of Scenedesmus sp. FSP3 was delayed for the following two days, then grew slowly.
When exposed to high CO 2 levels, microalgae strive to maintain their cytosolic neutrality through CO 2 tolerance mechanisms, such as (i) upregulating proton extrusion; (ii) inactivating the CCM; (iii) modifying the composition of the cell membrane to enhance its role as a proton barrier; and (iv) increasing ATP synthesis to provide bioenergy for the operation of ATP-driven transport proteins [19]. Scenedesmus sp. FSP3 had a high tolerance to CO 2 , growing well at under 30% CO 2 , which may reduce CO 2 toxicity in this way. The higher biomass productivity and high CO 2 fixation rate and CO 2 tolerance of Scenedesmus sp. FSP3 makes it a great candidate for converting industrial gas CO 2 into biomass. CO 2 solubilization enhancers facilitated high concentrations of CO 2 to be more bioavailable to the microalgae, resulting in faster biomass productivity and CO 2 fixation rate. It is reported that the CO 2 solubilization enhancers can either increase the solubility of CO 2 through their affinity with CO 2 [11] or by increasing the driving force of the gas-liquid mass transfer process [12]. The Scenedesmus sp. FSP3 supplemented with PC showed better growth and carbon dioxide absorption. In a previous study, 0.5 g L −1 NaHCO 3 was added to a culture of Chlorella sp. HS2 [10]; their biomass productivity reached 530 mg L −1 d −1 , which is a 45% increase compared to that of the control. Zhu et al. [11] added 1 mM PEG 200 to the medium, and the specific growth rate of Nannochloropsis oceanica reached a maximum of 1.41 d −1 , which was 21.5% higher than that obtained without PEG 200. Extensive research and the use of CO 2 solubilization enhancers will help to further promote CO 2 adsorption in tandem with microalgal bio-CCU [2].
Lutein, a photosynthetic auxiliary pigment, is closely associated with the light-harvesting complex (LHC) of photosynthetic apparatus, meaning the light intensity has a strong influence on it. Under low light conditions, lutein synthesis was enhanced in parallel with light-harvesting complex proteins to increase the amount of LHC and possibly capture lowincident light efficiently [13]. The same trend has been seen in previous studies; Cocccomyxa onubensis [22] was incubated at 50, 140 and 400 µmol m −2 s −1 , with higher lutein content observed at 50 µmol m −2 s −1 . Chlorella sorokiniana FZU60 [23] was grown (on day 4) under 150, 300, 450, 600, and 750 µmol m −2 s −1 light intensities, with the highest lutein content (9.81 mg g −1 ) produced under 150 µmol m −2 s −1 light intensity incubation, with the lowest (6.46 mg g −1 ) observed under 750 µmol m −2 s −1 . When the light intensity reached the appropriate condition, the content of lutein decreased. This finding could be attributed to the increased light intensity, under which the photosynthetic apparatus is oversaturated with the breakdown of excess LHCs [8]. However, when the light intensity then continued to increase, lutein accumulated as a cellular defense against photooxidative damage to the photosynthetic system [24]; Tetraselmis sp. CTP4 [25] was reported to undergo the same condition, with higher lutein content obtained in the presence of a high light intensity of 170 µmol m −2 s −1 when compared to a low light intensity of 33 µmol m −2 s −1 . Additionally, Desmodesmus sp. F51 [26] was grown at light intensities of 150, 300, 450, 600, and 750 µmol m −2 s −1 to assess the effect of light intensity on lutein accumulation, reaching a maximum lutein content of 5.05 mg g −1 at 600 µmol m −2 s −1 . When light intensity continues to increase, it causes serious damage to the microalgal cells. The effect of light intensity on lutein accumulation is species-specific. This result suggests that optimizing the availability of light is critical for lutein accumulation in microalgal cells.
Excessive salinity (of the medium) is detrimental to the growth of microalgae. The highsalt-concentration hazards for microalgae include the following: water loss, ion imbalance, the overproduction of reactive oxygen species, the enzyme inactivation of cellular ions, and osmotic pressure imbalances [27]. Salt stress leads to several biochemical and bioenergetic changes, such as increased lipid synthesis and energy production, changes in membrane permeability due to disruption of ion homeostasis, and elevated levels of reactive oxygen species (ROS), resulting in changes in cellular metabolism as the microalgae adapt to their environment in response to stress and attempt to restore homeostasis in vivo [28], which leads to changes in the levels of antioxidant compounds that scavenge highly toxic ROS. Lutein, an antioxidant pigment, also accumulates in the cells of microalgae. Ali et al. [29] found elevated antioxidant activity in Chlorella vulgaris when 10 g L −1 NaCl was added, with a significant increase in total carotenoids, including lutein. Bermejo et al. [30] reported that Coccomyxa onubensis growth was inhibited when 200-500 mM salt was present, but lutein production was significantly induced by up to 7.80 mg g −1 . The most suitable salt concentration for lutein accumulation is also species-specific. Excessive salt concentration affects microalgal cell activity in many aspects, thus affecting lutein accumulation, so finding the right salt concentration is also necessary for lutein production.
Through the combined action of light stress and salt stress, the content of lutein increased by 17.27% when compared to a single factor change; the combination of stress can further promote the accumulation of lutein in microalgal cells. Lutein productivity was higher than that reported in most of the related studies (Table 2), which was attributed to the rapid growth of microalgae during the first stage, which greatly shortened the incubation period. By using a two-stage culture method, efficient CO 2 fixation was achieved during the first stage, and high lutein production was achieved during the second. This proves that it is feasible to integrate lutein production and CO 2 fixation processes in microalgae when using a two-stage culture strategy.

Microalgae Species and Cultivation
The microalgal strain used in this study was Scenedesmus sp. FSP3, which was provided by Jo-Shu Chang (National Cheng Kung University, Taiwan). BG-11 medium was used for the cultivation of Scenedesmus sp. FSP3. Scenedesmus sp. FSP3 culture was grown in a 0.5 L modified Erlenmeyer flask and maintained at 25 ± 1 • C, illuminated with 90 µmol m −2 s −1 fluorescent light, and 5% CO 2 infusion at 0.1 vvm (volume gas per volume media per minute) flow rate, continuously (filtered through a 0.22 µm microporous filter). The light quantum meter was placed at the bottom of the incubator to measure the light intensity. After three days of cultivation, the culture solution was collected as an experimental seed. In the culture process, we used two reactors: an airlift reactor and a modified Erlenmeyer flask. The airlift reactor is basically a cylindrical glass bottle with a diameter of 5.5 cm and a height of 35 cm, with an effective volume of 0.5 L. There is a vent in the bottle that goes from the top to the bottom. The gas is aerated from the bottom to mix the microalgae. In the upper part, there is an opening for the addition and collection of the culture medium. In the second stage, the microalgae were cultivated in the 0.5 L modified Erlenmeyer flask with a diameter of 13 cm and a height of 16 cm. Ventilation and openings are the same as in airlift reactors. The reactors were incubated on racks with LED lights, which were illuminated from directly above. The intensity of the light can be adjusted by a knob.

Cultivate Model: Two-Stage Culture
Scenedesmus sp. FSP3 cells with an optical density (OD680 nm) of 0.5 were inoculated into BG-11 medium. In the first stage, Scenedesmus sp. FSP3 culture was grown in a 0.5 L airlift column photoreactor, illuminated with 90 µmol m −2 s −1 fluorescent light at 25 ± 1 • C. The optimal CO 2 concentration for cell growth was assessed; Scenedesmus sp. FSP3 was cultivated with 0.03% (air), 5%, 10%, 15%, 20%, 25% and 30% CO 2 concentrations, aerated at 0.1 vvm flow rate continuously, respectively. For better carbon sequestration, 5 mmol L −1 methanol, polyethylene glycol (PEG200), propylene carbonate (PC), polyethylene glycol dimethyl ether (NHD), and N-methylpyrrolidone (NMP) were selected as CO 2 solubilization enhancers and were added to the microalgal culture. When the microalgae growth reached the end of the exponential phase, the microalgal cells were transferred to the second stage of the culture method. In the second stage, the Scenedesmus sp. FSP3 culture was grown in a 0.5 L modified Erlenmeyer flask and with air infusion rate of 0.2 vvm, and the microalgae were subjected to light and salt stress. For optimization of light intensity, the microalgae were illuminated with 30, 90, 150, 210 µmol m −2 s −1 ; for the optimization of the NaCl concentrations, the BG-11 medium was supplemented with 0, 50, 100, 150, and 200 mmol L −1 of NaCl. The dynamic changes in lutein content were measured under different light intensities and NaCl concentrations, respectively.

Measurement of Cell Growth
The biomass concentration of Scenedesmus sp. FSP3 was calculated by the optical density of Scenedesmus sp. FSP3 at 680 nm. The relationship between OD 680 and the dry cell weight (DCW) of Scenedesmus sp. FSP3 was established by linear regression; the biomass concentration can be calculated by Equation (1).
where C is the biomass concentration (g L −1 ), and x is OD 680 . Overall biomass productivity (P overall , g L −1 d −1 ) was calculated from the variation in biomass concentration (∆C, g L −1 ) within a cultivation time (∆t, d), according to Equation (2):

Measurement of Carbon Dioxide Biofixation Rate
The CO 2 fixation rate, R CO 2 (g L −1 d −1 ), was calculated using Equation (3): where f C is the carbon content of the microalgal cells (%, w/w), and M C is the molecular weight of the carbon. M CO 2 is the molecular weight of the CO 2 . The value of f C becomes 0.51 by utilizing CH 1.83 O 0.48 N 0.11 P 0.01 as the molecular formula for the microalgal biomass. The input and output gas of the microalgae cultures were collected by a 0.5 L airbag; then, the CO 2 concentration was measured by a CO 2 analyzer. CO 2 gas absorptivity (η CO 2 ) was calculated according to Equation (4): where C CO 2 , in (%), is the CO 2 volume fraction of the inlet gas, and C CO 2 , out (%), is the CO 2 volume fraction of the effluent gas [34].

Extraction and Determination of Lutein
The lutein was extracted from the Scenedesmus sp. FSP3 cells using a modified method by Xie et al. [26]. In brief, 10 mg of lyophilized cells and glass beads of 0.1 mm diameter were mixed with an aqueous potassium hydroxide solution (0.5 mL, 60% w/w KOH). The microalgal cells were crushed at 65 Hz for 10 min by a grinder. The mixture was then placed in a 40 • C water bath for 40 min. The lutein was extracted by ethyl ether, and the cell fragmentation and centrifugation were repeated four-five times. The supernatant was collected until the extract solution was colorless and the lutein was completely extracted. We evaporated all of the ethyl ethers in supernatants with nitrogen and added 3 mL of acetone to redissolve the residue.

Response Surface Methodology for Lutein Yield
The second stage of incubation involved the optimization of light intensity and salt concentration in promoting lutein production. Moreover, the cultivation time also had a significant effect on lutein production. Therefore, these three factors were selected: cultivation times of two, four, and six days, light intensity levels of 60, 110, 160 µmol m −2 s −1 , and NaCl concentration levels of 100, 150, and 200 mmol L −1 . The software Minitab 19 was used to design the Box-Behnken Design (BBD) for these three factors.

Conclusions
By using a two-stage culture method, the maximum biomass productivity (0.58 g L −1 d −1 ) and carbon fixation rate (1.09 g L −1 d −1 ) were achieved during the first stage; the addition of CO 2 solubilization enhancers promoted the microalgae bio-CCUS. Scenedesmus sp. FSP3 reached a maximum lutein content of 6.45 mg g −1 and a lutein yield of 2.30 mg L −1 d −1 under the combination of light stress and salt stress during the second stage. This study integrates the lutein production process of microalgae with CO 2 fixation, which improves overall economic efficiency and makes a positive contribution to the commercial production of lutein.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules27217497/s1. Figure Table S1: Experimental design of response surface methodology and experimental results.