Recent Advances of Silver-Based Coordination Polymers on Antibacterial Applications

With the continuous evolution of bacteria and the constant use of traditional antibiotics, the emergence of drug-resistant bacteria and super viruses has attracted worldwide attention. Antimicrobial therapy has become the most popular and important research field at present. Coordination Polymer (CP) and/or metal-organic framework (MOF) platforms have the advantages of a high biocompatibility, biodegradability, and non-toxicity, have a great antibacterial potential and have been widely used in antibacterial treatment. This paper reviewed the mechanism and antibacterial effect of three typical MOFs (pure Ag-MOFs, hybrid Ag-MOFs, and Ag-containing-polymer @MOFs) in silver-based coordination polymers. At the same time, the existing shortcomings and future views are briefly discussed. The study on the antibacterial efficacy and mechanism of Ag-MOFs can provide a better basis for its clinical application and, meanwhile, open up a novel strategy for the preparation of more advanced Ag-contained materials with antibacterial characteristics.


Introduction
At present, people's yearning for a better life makes people pay more and more attention to disease and health. However, pathogenic bacteria have a high rate of morbidity and mortality properties. Various antibiotics are used globally to treat infections resulting from bacteria. This wide use results in the critical resistance of bacteria against the drugs used, super viruses, and the lack of effective treatment methods. Therefore, searching for a new treatment or antibacterial drugs has become the hottest topic [1].

Silver-Based MOFs
Pure Ag-MOFs refer to materials with a three-dimensional structure, prepared certain methods (in-situ growth, hydrothermal reaction, etc.) using silver as the nodes and organic linkers. Mainly, MOFs are used as "reservoirs" of Ag cations to the controllable release of metal ions. The presence of silver can provide certain a terial activities to the MOFs, which is higher than the commercial silver nanoparticl MOF system can control the release rate of silver and avoid the toxicity caused sudden release of silver. Based on pure Ag-MOFs, the antibacterial effect of MOFs increased, or other synergies can be obtained by adding certain modifiers or loadin microbial agents. These polymer materials (such as chitosan, polyvinyl alcohol, gra oxide, etc.) have unique properties (i.e. anticoagulant, high biocompatibility, hydro antibacterial, etc.). Their addition allows Ag-MOFs to have a more accurate antiba activity than the pure Ag-MOFs, and can also improve the antibacterial effect.
Silver-containing polymer @ MOFs mean that on the other MOF structure lo silver and chemical materials obtain the antibacterial properties or have other spec formances. These MOFs usually have some characteristics, such as a high poro strong stability, a good biological activity, they prevent the Ag ions reunion and a terial properties, etc., which can help Ag to have a greater antibacterial effect. At th time, the other MOFs, as the carrier of silver, not only have a certain antibacterial

Silver-Based MOFs
Pure Ag-MOFs refer to materials with a three-dimensional structure, prepared using certain methods (in-situ growth, hydrothermal reaction, etc.) using silver as the metal nodes and organic linkers. Mainly, MOFs are used as "reservoirs" of Ag cations to ensure the controllable release of metal ions. The presence of silver can provide certain antibacterial activities to the MOFs, which is higher than the commercial silver nanoparticles. The MOF system can control the release rate of silver and avoid the toxicity caused by the sudden release of silver. Based on pure Ag-MOFs, the antibacterial effect of MOFs can be increased, or other synergies can be obtained by adding certain modifiers or loading antimicrobial agents. These polymer materials (such as chitosan, polyvinyl alcohol, graphene oxide, etc.) have unique properties (i.e., anticoagulant, high biocompatibility, hydrophilic, antibacterial, etc.). Their addition allows Ag-MOFs to have a more accurate antibacterial activity than the pure Ag-MOFs, and can also improve the antibacterial effect.
Silver-containing polymer @ MOFs mean that on the other MOF structure load, the silver and chemical materials obtain the antibacterial properties or have other special performances. These MOFs usually have some characteristics, such as a high porosity, a strong stability, a good biological activity, they prevent the Ag ions reunion and antibacterial properties, etc., which can help Ag to have a greater antibacterial effect. At the same time, the other MOFs, as the carrier of silver, not only have a certain antibacterial ability, but also can improve the loading amount of silver, and thus improve the release amount of the silver ions.

Pure Ag-MOFs
The three-dimensional structure of the silver-MOFs, Ag 6 (m-O 3 PC 6 H 4 CO 2 ) 2 , MOF-1 has been prepared through the hydrothermal reaction of Ag (NO 3 ) and m-phosphonobenzoic acid. The organic part is constituted by 3-phosphonobenzoic acid, which is a rigid organic molecule and is classified as a hard base; hence, the silver-MOF-1, acts as a reservoir of Ag + , which possesses an intermediate stability that exhibits the release of the Ag + ions with the consequent bactericidal effect, and can be utilized against the Gram-positive S. aureus (minimum bactericidal concentration (MBC)) value, 50-70 µM) and the Gram-negative strains of Pseudomonas aeruginosa (P. aeruginosa) (MBC value, 20-30 µM). Furthermore, it was shown that MOF-1 did not exhibit a significant cytotoxicity [98].
Quaternized carboxylate ligands have a good water dispersibility and stability, and they are used as ligands to prepare 3D Ag-MOF-2, [Ag 2 (Cedcp)] n , (H 3 CedcpBr denotes N-(carboxyethyl)-(3,5-dicarboxyl)-pyridinium bromide), which showed a good stability and solubility, and could release Ag + better, resulting in the strong antibacterial activity towards the Gram-negative and Gram-positive bacteria strains. The Ag-MOF-2 mainly destroys the bacterial membrane through the synergistic effect of the ligand's characteristic aromatic ring and positively charged pyridine and the release of Ag + , resulting in bacterial death. In addition, the MOF-2 showed little hemolytic activity on mouse erythrocytes and exhibited an excellent in vitro biocompatibility [99].
cules 2022, 27, x FOR PEER REVIEW 5 of negative bacteria, and the MIC values obtained for the MOF-6 (4-5 µg mL −1 ) were pref entially lower than those of MOF-7 (20 µg mL −1 ). The observed antibacterial activity MOF-6 and MOF-7 is due to the presence of bioactive silver nodes. The highest antimic bial activity of MOF-6 has been reported than that of the corresponding silver MOFs d rived from PTA=O, and stronger than other silver(I) salts AgNO3 ( Figure 2) [103]. Two Ag-MOFs, Ag5(PYDC)2(OH) (MOF-8) and [Ag2(O-IPA)(H2O)·(H3O)] (MOFwere synthesized using hydroxyl and pyridyl-contained aromatic-carboxylic acids as l ands (HO-H2IPA = 5-hydroxyisophthalic acid and H2PYDC = pyridine-3, 5-dicarboxy acid). Utilizing the MOFs as a carrier, the Ag + in both compounds diffuse to the bacter The pure MOF-4 ( Figure 2B) [Ag 2 (µ 4 -PTA)(µ 4 -mal)] n has been synthesized using PTA as a main fundamental block and flexible aliphatic dicarboxylic acids (malonic (H 2 mal) acids) as an ancillary ligand source. The silver(I) coordination polymers feature a solubility in water and show critical antibacterial and antifungal activities towards the selected strains of the Gram-negative MIC of E. coli and P. aeruginosa (6 and 7 µg mL −1 , respectively) and the Gram-positive MIC of S. aureus (8 µg mL −1 ) bacteria and yeast (the MIC of C. albicans is 30 µg mL −1 ). The mechanism relates to the coordination sphere around each silver atom that controls the compounds' antimicrobial efficiencies. A slow Ag + ion releasing into the solution would be expected in the presence of an organic ligand containing O and N atoms as weaker donating centers. This can increase the replacement of organic ligands by the biological moieties to release the Ag(I) cations [101].
Two Ag-MOFs, Ag 5 (PYDC) 2 (OH) (MOF-8) and [Ag 2 (O-IPA)(H 2 O)·(H 3 O)] (MOF-9), were synthesized using hydroxyl and pyridyl-contained aromatic-carboxylic acids as ligands (HO-H 2 IPA = 5-hydroxyisophthalic acid and H 2 PYDC = pyridine-3, 5-dicarboxylic acid). Utilizing the MOFs as a carrier, the Ag + in both compounds diffuse to the bacterial surface and release the Ag + ions, and the ion channels are destroyed. The Ag + ions may interact with the thiol groups of protein, which can inactivate vital enzymes and disrupt the bacterial membrane's integrity and permeability. In addition, a functional group of organic ligands in the MOFs can bond with the cations in the cell, such as Ca 2+ and Mg 2+ . The reaction-oxygen generated in the cell results in the modifications and fragmentation of DNA. It showed a high antibacterial activity against the Gram-negative bacteria (E. coli) and the Gram-positive bacteria (S. aureus). Furthermore, it has a good biocompatibility with blood cells. The relevant data show that the MIC of the MOFs synthesized by this method is 5-15 ppm for E. coli and 10-20 ppm for S. aureus, and MOF-8 and MOF-9 exhibit diameters of the inhibition zones (ZOI) in 17.0 and 20.0 mm for E. coli. The diameters of the inhibition zones against S. aureus for MOF-8 and MOF-9 are 14.0 and 16.0 mm, respectively, and they are larger than the Ag-NPs [104].
The MOF-10, [AgL] n ·nH 2 O, (L=4-Cyanobenzoate) exhibits a good antibacterial performance against the S. mutans (UA159), F. nucleatum (ATCC 10953, Fn), and P. gingivalis oral bacteria. The bactericidal ability is enhanced as the concentration of the metal ions increases. Compared with the traditional antibacterial materials, such as silver nitrate (AgNO 3 ), the MOF-10 dispenses better slow-release bactericidal properties. The sterilization property of the MOF containing the Ag + ions is better than that of the MOF with Zn 2+ ions [105].
The metal nodes of the Ag + ions and the linkers made of redox-active naphthalenediimide (NDI) derivatives could act as excellent radical-doped Ag-MOF antibacterial materials, such as, the two reported MOFs, such as MOF-11 Ag (NDI-1) 0.5 (H 2 O) and MOF-12 Ag 7 (NDI-2) 1.5 (CH 3 S) 4 (DMSO) 3 (DMSO). Because of the synergistic antibacterial effects of the Ag + ions and the reactive organic radicals, those MOFs displayed a broad-spectrum antimicrobial activity against the multi-drug-resistant bacteria; the reported inhibiting rate was more than 98.74%. The colony inhibition ratio (IR) of the MOF-11 and MOF-12 against the Gram-negative and Gram-positive strains is about 100% and 99.90%, respectively, which is higher than AgNO 3 (IR value: 89.29-94.92%) [106].
The Ag-MOFs types, the antibacterial activity against the different bacteria, and their experimental conditions have been tabulated in Table 1. The pure Ag-MOFs were prepared using silver ions as a metal center and the relevant organic systems; the organic ligands effectively wrap the active sites of the Ag metal ions in the framework. The organic ligand is evenly distributed across the overall material, enabling a sustained release of the metal ions to avoid the toxicity caused by a burst release of the metal ions. The Ag-MOFs showed a high antibacterial activity against the Gram-positive and Gram-negative bacteria.
The MOFs-3, 4, 5, 6, and 7, constructed by the linkers 1,3,5-triaza-7-phosphaadamantane (PTA) or 1,3,5-triaza-7-phosphaadamantane-7-sulfide (PTA=S), which possess the feature of water-solubility and air-stability, endow the Ag-MOFs with a better bioavailability, and physiological media by achieving the lowest MIC values and a very high ZOI. As a compatible polymer of the MOFs, PTA can synthesize three-dimensional topology structures and slowly release the Ag + ions into the solution due to the O and N-donor ligands exerting only a weak binding affinity for the silver, increasing the probability of the organic ligand replacement by the biological moieties. Moreover, the Ag-O and Ag-N bonds have a better biocompatibility than the Ag-S and Ag-P bonds [94,107,108]. These characteristics are favorable for the Ag-MOFs to release their silver ions, and then, with their breaking ability to balance the ions and disrupting the integrity of the cell membrane, with the cell internalization of the Ag + ions, they interact with the fragmentation of DNA. At the same time, the structural changes, such as a 3D network, can give the MOFs a higher stability and be more conducive to the Ag + ion antibacterial activity. The 3D structure can also act as a depository for the Ag + cation, ensuring the Ag's slow and continuous release.
The strong antibacterial activity of the Ag-MOFs is correlated to the donor site of the weak ligand, their binding force, and the synergistic effect of the ligand. The presence of the organic ligands can increase the stability of the silver ions and thus enhance the antibacterial activity. The MOFs-11 and 12 have a very high and significant effect on the inhibition of the Gram-positive and Gram-negative bacteria, and the inhibition rate (IR) is more than 99.5%. The organic ligand naphthalenediimid (NDI)) of MOF-11 and MOF-12 have stable radical-doped coordination compounds that produce organic radicals by unconventional lone pair-Π interactions and generate the synergistic antibacterial effects of the Ag + ions and the reactive organic radicals [109].
The released silver ions are known as the main reason for the antibacterial activity of the pure Ag-MOFs. The main purpose is to release the silver ions from the MOF structure, whether it is the weak binding force between the ligand and silver metal or the synergistic antibacterial action with silver, which is conducive to a series of reactions of Ag + ions on the bacterial membrane and changes the surrounding environment of the cells, and then destroy the ion channels. More importantly, the silver ions react with the thiol groups and the intracellular substances, failing the cell transcription and replication, which ultimately ends with the death of the bacteria.

Hybrid Ag-MOFs
Huang et al. designed a novel platelet membrane-camouflaged material PLT@ Ag-MOF-Vanc (MOF-13). The MOF-13 can release silver ions and drugs through the pH regulation, avoiding a premature drug release in the circulatory system. The MOF-13 showed an obvious inhibitory effect on MRSA, the MIC of MOF-13 was 0.5 µg mL −1 , and the effect was better than with the Ag-MOF-Vanc and vancomycin alone. The antibacterial mechanism may be related to the synergistic reaction of physics and chemistry, which includes targeting MRSA via PLTm; the intracellular bacterial metabolism interfering effect; the catalytic effect in the ROS production; the damaging of the cell membrane integrity; and the inhibiting effect on the formation of a biofilm [110,111] (Figure 3). was better than with the Ag-MOF-Vanc and vancomycin alone. The antibacterial me nism may be related to the synergistic reaction of physics and chemistry, which inclu targeting MRSA via PLTm; the intracellular bacterial metabolism interfering effect; catalytic effect in the ROS production; the damaging of the cell membrane integrity; the inhibiting effect on the formation of a biofilm [110,111] (Figure 3). Using silver acetate as the metal source and 2-aminoterephthalic acid as the org linker, a new MOF-14 with a higher antibacterial effect, namely Ag-MOF@TFN (thinnanocomposite) was synthesized. The MOF-14 can completely inactivate and degrad coli and S. aureus, and attributed to the mortality rates of approximately more than 90% materials embedded within the polyamide matrix and forwarding osmosis membra to improve their antifouling and antibacterial properties. While the most probable a bacterial mechanism is related to the release of the Ag + , other possible antibacterial m anisms proposed for the Ag-MOFs' biocidal properties are (i) direct attachment to the terial cells, infiltration, and the physical destruction of the cell membrane and (ii) the direct generation of the reactive oxygen species which trigger damage to the bacteria structure [112].
In an article, S. Fatemeh Seyedpour et al. reported a new antifouling TFC (thincomposite) polyamide membrane by surface anchoring via the in-situ assembly of the ver-based MOFs. The MOF-15 (Ag-MOFs @TFC) have antibacterial properties aga gram-negative bacteria (P.aeruginosa strains), and a bacterial mortality rate (MR) of proximately 100% was attained. The antibacterial mechanism of the Ag-MOFs inclu several pathways; (i) direct adherence of the Ag ions to the bacterial cells and permea into them, (ii) the release of Ag + from the dissolved Ag NPs and then penetration to Using silver acetate as the metal source and 2-aminoterephthalic acid as the organic linker, a new MOF-14 with a higher antibacterial effect, namely Ag-MOF@TFN (thin-film nanocomposite) was synthesized. The MOF-14 can completely inactivate and degrade E. coli and S. aureus, and attributed to the mortality rates of approximately more than 90% of materials embedded within the polyamide matrix and forwarding osmosis membranes, to improve their antifouling and antibacterial properties. While the most probable antibacterial mechanism is related to the release of the Ag + , other possible antibacterial mechanisms proposed for the Ag-MOFs' biocidal properties are (i) direct attachment to the bacterial cells, infiltration, and the physical destruction of the cell membrane and (ii) the indirect generation of the reactive oxygen species which trigger damage to the bacteria cell structure [112].
In an article, S. Fatemeh Seyedpour et al. reported a new antifouling TFC (thin-film composite) polyamide membrane by surface anchoring via the in-situ assembly of the silver-based MOFs. The MOF-15 (Ag-MOFs @TFC) have antibacterial properties against gram-negative bacteria (P.aeruginosa strains), and a bacterial mortality rate (MR) of approximately 100% was attained. The antibacterial mechanism of the Ag-MOFs includes several pathways; (i) direct adherence of the Ag ions to the bacterial cells and permeating into them, (ii) the release of Ag + from the dissolved Ag NPs and then penetration to the bacteria inside, and (iii) the indirect production of reactive oxygen species by the Ag NPs and Ag + . In other words, MOFs can be a reservoir of metal ions, such as silver (Ag + ), and the chronic depletion of the metal ions by the destruction of the framework can create a sustainable antibacterial activity [113].
The Ag-MOFs (Aand Gram-negative bacteria, respectively, and the maximum diameter of the inhibition zone of S. aureus and E. coli is 12.1 and 9.7 mm, respectively. The MOF-16 has an anticoagulation effect and a good biocompatibility because of the presence of PVA and CS. The MOF-16 can release Ag + to strongly attract the enzyme protein in bacteria and quickly bind together to destroy the bacterial cell membrane. The Ag + can also form a reactive oxygen species (ROS) that further attacks the cell membranes [114].
The MOF-17 (Ag-MOF−CQDs) were synthesized by the nanocomposites and g 5 (PYDC) 2 (OH)) (PYDC=Pyridine-3, 5-Dicarboxylic Acid) was synthesized under the modified hydrothermal, and then the polyvinyl alcohol (PVA) hydrogel modified the Ag-MOFs as the inner layer of the membrane, and polyvinyl alcohol/chitosan (PVA/CS) were combined as the outer layer of the membrane to attain the hybrid material PVA/Ag-MOFs @CS (MOF-16). The outer layer of the double-layered dressing a has good biocompatibility, while the inner layer has a high antibacterial activity and avoids direct contact with cells. The MOF-16 possessed an antimicrobial activity against S. aureus and E. coli, which represent the Gram-positive hybrid materials of Ag-1,3,5-benzenetricarboxylic with Sand N-carbon quantum dots (CQDs). The MOF-17 possessed a great antibacterial activity against the representative Gram-positive (Bacillus subtilis (B. subtilis)) and the Gram-negative (E. coli) bacterial strains. The composite formation fostered a synergistic effect that enhanced their antibacterial activity, compared to their new components. The ZOI of E. coli and B. subtilis is 11-15 nm, and 9.5-13.5 nm, respectively. In addition to the release of metallic silver, the bactericidal activity was linked mainly to the surface charge of the CQDs. These materials interacted with the surface of the bacterial cells through electrostatic forces and thus disrupted the integrity of the cell membrane, leading to bacterial death [115].
The MOF-21, CS/SS/Ag @MOFs-GO, is a silver (Ag)-based metal-organic framework (Ag-MOF) that is embellished with graphene-oxide (GO), whose biocidal activity is higher than those of the Ag-MOFs and the GO nanomaterials. The Ag @MOF-GO impregnated into sericin/chitosan (SS/CS) hydrogels are successfully synthesized through a green strategy. These materials have special properties in improving the cell adhesion, antibacterial activity, biocompatibility, water retention, and antioxidants. The MOFs possess dual antibacterial effects, which contain GO and Ag-MOFs. The Ag@ MOF-GO continuously released the Ag + to the surrounding environment, which caused an interaction between the Ag + and the thiol group protein to destroy the integrity of the bacterial membrane. The interaction between the O-containing functional groups of CS/SS/Ag @MOFs-GO and the bacterial lipopolysaccharide promoted the interaction between the Ag + and bacteria to destroy the bacterial cells. In addition, the GO's antibacterial properties originate from the physical and chemical interaction between the GO and bacterial cell membranes. The sharp edges of the GO damage the bacterial cell membranes [117]; the oxidative stress of the GO damages E. coli cells [118]; the GO can generate superoxide anions to damage the bacteria's cellular membrane [119,120] (Figure 4).   Table 1 reports all of the relevant data obtained from antibacterial tests, in the case the hybrid Ag-MOFs 13-23, with their antibacterial effect and the mechanism related each MOF. Table 1 shows that the hybrid silver material had an excellent antibacter activity against the Gram-negative and Gram-positive bacteria strains, with a prefer tially greater activity in the case of the Gram-negative strain. This is due to their cell-w structural differences with the thick peptidoglycan layer protecting the plasma of membrane. as the MOFs-14, 15, and MOF-19 were modified by the TFC membrane to fo the nanocomposite film, to improve the antibacterial properties, the water penetrati and the salt selectivity of the film. Those materials possessing superior antibacterial pro erties were probably related to the release of the Ag + into the solution and the destruct of the framework, the Ag + interacting with lipotropic acid, and the hydroxyl groups of peptidoglycan membranes.
The antibacterial effect of hybrid the Ag-MOFs not only comes from the release of but also has a certain relationship with the hybrid material. The Ag-MOFs have multi effects by transferring some material with other effects (such as targeting, increasing s bility, biocompatibility, and antibacterial effects) onto MOFs. In the MOF-17, the GO h a certain antibacterial ability, and incorporating it with the Ag-MOFs can significan achieve the antibacterial effect. In addition, we can not only modify some materials Ag-MOFs but we can load some antibacterial drugs on the MOF to increase its antibac rial spectrum for a better antibacterial effect. The MOFs-13 and 24 both have a good an bacterial effect on the traditional Gram-positive and Gram-negative bacteria and hav certain therapeutic effect on MRSA and other drug-resistant bacteria. The mechanism m  Table 1 reports all of the relevant data obtained from antibacterial tests, in the case of the hybrid Ag-MOFs 13-23, with their antibacterial effect and the mechanism related to each MOF. Table 1 shows that the hybrid silver material had an excellent antibacterial activity against the Gram-negative and Gram-positive bacteria strains, with a preferentially greater activity in the case of the Gram-negative strain. This is due to their cell-wall structural differences with the thick peptidoglycan layer protecting the plasma of the membrane. as the MOFs-14, 15, and MOF-19 were modified by the TFC membrane to form the nanocomposite film, to improve the antibacterial properties, the water penetration, and the salt selectivity of the film. Those materials possessing superior antibacterial properties were probably related to the release of the Ag + into the solution and the destruction of the framework, the Ag + interacting with lipotropic acid, and the hydroxyl groups of the peptidoglycan membranes.
The antibacterial effect of hybrid the Ag-MOFs not only comes from the release of Ag but also has a certain relationship with the hybrid material. The Ag-MOFs have multiple effects by transferring some material with other effects (such as targeting, increasing stability, biocompatibility, and antibacterial effects) onto MOFs. In the MOF-17, the GO has a certain antibacterial ability, and incorporating it with the Ag-MOFs can significantly achieve the antibacterial effect. In addition, we can not only modify some materials on Ag-MOFs but we can load some antibacterial drugs on the MOF to increase its antibacterial spectrum for a better antibacterial effect. The MOFs-13 and 24 both have a good antibacterial effect on the traditional Gram-positive and Gram-negative bacteria and have a certain therapeutic effect on MRSA and other drug-resistant bacteria. The mechanism may be related to the release of the Ag ions, damage to the cell membrane integrity, interfering with the intracellular metabolism of the bacteria, and inhibiting the biofilm formation. Their antibacterial ability comes from the release of silver ions and the action of the antibacterial drugs themselves, which provides a greater practical value for the clinical application.
Simultaneously, the Ag-based material would release the Ag + and diffuse into the interior cytoplasm of the cells to interact with lipotropic acid, the hydroxyl groups of the peptidoglycan membranes, and the phosphate groups of phospholipid membranes [121,122]. Meanwhile, the probable interaction of the Ag(I) ions with DNA and the thiol groups of proteins may damage enzymes and disrupt the integrity and permeability of the bacteria and the catalytic production of ROS [123]. The release of Ag + .
[100] The weak binding tendency of O and N donor atoms toward the center helps the slow release of Ag(I). [101]
[106] The synergistic activity of the releasing Ag + ions and SD. [124]

Silver-Containing Polymer @ MOF
A preferential antibacterial activity of the insoluble silver sulfadiazine has been reached due to the co-delivery of the superfine Ag NPs with solubilized sulfadiazine (SD) using the carrier cyclodextrin metal-organic frameworks (CD-MOFs). The MOF-24 (SD/Ag@ CD-MOF) critically strengthens the antibacterial effect, which can increase the release of Ag + and SD together to produce a synergistic antibacterial action. The hydrophilic CD-MOF can easily dissolve within exudates in the wound region to release the drug and prevent the aggregation of the nano-silver particles, which can enhance the antibacterial effect. The MIC of the MOF-24 against E. coli and S. aureus is 4 and 6 µg·mL −1 and the MBC are 8 and 64 µg·mL −1 , respectively [126].
The MOF-25 Ag-Phy @ZIF-8@HA is a pH-responsive antimicrobial composite nanomaterial prepared by encapsulating the Ag NPs in the ZIF-8, accompanied by the embedding of physcion (Phy). It has an excellent antimicrobial ability against E. coli and S. aureus ( Figure 5C,D). The ZIF-8 possesses the special feature that the Zn 2+ ion is a beneficial element for humans and the ligands dimethylimidazole is also a good bacterial inhibitor. The ZIF-8 can be disintegrated slowly in a different environment, which is good for releasing drugs and other materials. The antibacterial mechanism was due to the efficient synergistic effect of the ZIF-8, silver, Phy, and the bacterial growth secretes hyaluronidase, which breaks down HA, causing the surrounding environment to become acidified ( Figure 5B). The pH response is triggered to cause the crack in the nanomaterial to release the Ag NPs and Phy, which further releases the Ag + ions and destroys the bacterial membrane's ionic channel and permeability [132]. The MOF-26, GOD/Ag @ZIF-HA, the controlled encapsulation of a large-size sin Ag nano-cube (50 nm) in the zeolitic imidazolate frameworks (ZIFs), accompanied by embedding of glucose oxidase (GOD), and hyaluronic acid (HA), were coated into MOF. The obtained MOF-26 could act as an Ag nano-factory to generate ultrasmall NPs, which is good for releasing the Ag ions. Specifically, the GOD-induced genera H2O2 in glycolysis continuously decomposed silver into Ag + and ultrasmall Ag N where the biocompatible ZIF-8 acted as the porous support for Ag + and the Ag NP mation. The HA possesses a good biocompatibility and target ability that is good for MOFs to achieve the antibacterial effect. The MOF-26 could completely suppress growth of the two model bacteria strains at low concentrations (E. coli. for 5 µg mL − aureus for 10 µg mL −1 ). The mechanism of MOF-26 may be related to the particle siz the Ag, the small particle size allowed into the cells and released abundant Ag + , wh caused the cell death in terms of oxidative stress, the mitochondrial membrane, and cell cycle progression [133] (Figure 6). The MOF-26, GOD/Ag @ZIF-HA, the controlled encapsulation of a large-size single Ag nano-cube (50 nm) in the zeolitic imidazolate frameworks (ZIFs), accompanied by the embedding of glucose oxidase (GOD), and hyaluronic acid (HA), were coated into the MOF. The obtained MOF-26 could act as an Ag nano-factory to generate ultrasmall Ag NPs, which is good for releasing the Ag ions. Specifically, the GOD-induced generated H 2 O 2 in glycolysis continuously decomposed silver into Ag + and ultrasmall Ag NPs, where the biocompatible ZIF-8 acted as the porous support for Ag + and the Ag NP formation. The HA possesses a good biocompatibility and target ability that is good for the MOFs to achieve the antibacterial effect. The MOF-26 could completely suppress the growth of the two model bacteria strains at low concentrations (E. coli for 5 µg mL −1 , S. aureus for 10 µg mL −1 ). The mechanism of MOF-26 may be related to the particle size of the Ag, the small particle size allowed into the cells and released abundant Ag + , which caused the cell death in terms of oxidative stress, the mitochondrial membrane, and the cell cycle progression [133] (Figure 6).
The Ag @Ni-MOF-27 was prepared by loading silver onto the Ni-MOF. Despite the good antibacterial affinity of the Ni-MOF nanosheets, the MOF-27 exhibited an obvious antibacterial activity against the four microbes (B. subtilis, E. coli, P. aeruginosa, and C. albicans) after 48 h of incubation. It is due to the synergistic effect of the Ni-MOF and Ag cations. The chelation could reduce the polarity of the nickel ions and increase the lipophilicity of the nickel atom, which was beneficial to enhancing the membrane penetration of the MOFs. Meanwhile, the MOFs can act as a reservoir for the metal cations. The released cations can change the microbes' ionic nature and destroy the ion channels. The released Ag(I) ions can interact with the thiol groups in proteins [134] and can inactivate the respiratory enzymes and disrupt the bacterial membrane integrity and the permeability [124]. Moreover, the metallic Ag can induce oxidative stress on the microbes and damage the membrane [81]. The Ag @Ni-MOF-27 was prepared by loading silver onto the Ni-MOF. Despite t good antibacterial affinity of the Ni-MOF nanosheets, the MOF-27 exhibited an obvio antibacterial activity against the four microbes (B. subtilis, E. coli, P. aeruginosa, and C. bicans) after 48 h of incubation. It is due to the synergistic effect of the Ni-MOF and A cations. The chelation could reduce the polarity of the nickel ions and increase the lip philicity of the nickel atom, which was beneficial to enhancing the membrane penetrati of the MOFs. Meanwhile, the MOFs can act as a reservoir for the metal cations. The r leased cations can change the microbes' ionic nature and destroy the ion channels. T released Ag(I) ions can interact with the thiol groups in proteins [134] and can inactiva the respiratory enzymes and disrupt the bacterial membrane integrity and the permeab ity [124]. Moreover, the metallic Ag can induce oxidative stress on the microbes and dam age the membrane [81].
The copper-based polymer-MOF-28, poly Cu-MOF @Ag, is an efficient scaffold loa ing silver nanoparticle, which was used as a preferential antibacterial agent. The MOFhybrid contains lower copper nodes and a higher Ag, showing the amount of Ag + an Cu 2+ ions to enhance the biocompatibility. The MOF-28 can efficiently kill E. coli (M value 10 µg mL −1 ) and S. aureus (MIC value 10 µg mL −1 ) via damaging the cell integrity the produced ROS and the disruption of the membrane of the bacteria. The release of t Ag + ions in the presence of the bacterial cells could be increased because the Ag NPs c interact with the sulfur-containing proteins of the cell walls (Figure 7) [125]. The copper-based polymer-MOF-28, poly Cu-MOF @Ag, is an efficient scaffold loading silver nanoparticle, which was used as a preferential antibacterial agent. The MOF-28 hybrid contains lower copper nodes and a higher Ag, showing the amount of Ag + and Cu 2+ ions to enhance the biocompatibility. The MOF-28 can efficiently kill E. coli (MIC value 10 µg mL −1 ) and S. aureus (MIC value 10 µg mL −1 ) via damaging the cell integrity by the produced ROS and the disruption of the membrane of the bacteria. The release of the Ag + ions in the presence of the bacterial cells could be increased because the Ag NPs can interact with the sulfur-containing proteins of the cell walls (Figure 7) [125].
The MOF-29, Ag-MIL-101 (Cr), was prepared by containing the silver nanoparticles on the MIL-101(Cr). The MOF-29 have an obvious antibacterial phenomenon against both the Gram-negative (E. coli and P. aeruginosaand) and Gram-positive (S. aureus) bacteria strains, and the MIC of the MOF-29 to E. coli, P. aeruginosaand, and S. aureus is 1 mg L −1 . The MIL-101 are a porous organic-inorganic hybrid with a high surface area and a well-defined pore structure. The MOFs can be used to stabilize the Ag metal in an adjustable size. Hence, the antibacterial mechanism may be related to the particle size, the uniform distribution, and the absence of aggregation of the Ag metal. Because of the presence of the MIL-101, the Ag ions are more stable in their structure and can be better released in the application [126]. The MOF-29, Ag-MIL-101 (Cr), was prepared by containing the silver nanopartic on the MIL-101(Cr). The MOF-29 have an obvious antibacterial phenomenon against bo the Gram-negative (E. coli and P. aeruginosaand) and Gram-positive (S. aureus) bacte strains, and the MIC of the MOF-29 to E. coli, P. aeruginosaand, and S. aureus is 1 mg L The MIL-101 are a porous organic-inorganic hybrid with a high surface area and a we defined pore structure. The MOFs can be used to stabilize the Ag metal in an adjustab size. Hence, the antibacterial mechanism may be related to the particle size, the unifo distribution, and the absence of aggregation of the Ag metal. Because of the presence the MIL-101, the Ag ions are more stable in their structure and can be better released the application [126].
MOF-5 was prepared using the hydrothermal method, and the Ag@MOF-5 (MO 30) nanoplates were obtained to improve the Ag nanoparticles' antibacterial and disp sion activities. The antibacterial activity of the MOF-5 has been limited because of the cation of the Zn 2+ ion in the MOF-5 skeleton. However, MOF-30 showed a better antib terial activity against E. coli (ZOI = 16.05 mm) and S. aureus (ZOI = 14.63 mm), which due to the addition of the silver nanoparticles that make it easier for the metal ions diffuse into the bacteria, destroy the bacterial membranes and inhibit the cell divisi [123,134].
These γ-cyclodextrin metal-organic frameworks (CD-MOFs) possess a good wa solubility and biocompatibility, which can act as a template to prepare the Ag@CD-MO These types of materials can achieve a dual function in reducing the particle size and e MOF-5 was prepared using the hydrothermal method, and the Ag@MOF-5 (MOF-30) nanoplates were obtained to improve the Ag nanoparticles' antibacterial and dispersion activities. The antibacterial activity of the MOF-5 has been limited because of the location of the Zn 2+ ion in the MOF-5 skeleton. However, MOF-30 showed a better antibacterial activity against E. coli (ZOI = 16.05 mm) and S. aureus (ZOI = 14.63 mm), which is due to the addition of the silver nanoparticles that make it easier for the metal ions to diffuse into the bacteria, destroy the bacterial membranes and inhibit the cell division [123,134].
These γ-cyclodextrin metal-organic frameworks (CD-MOFs) possess a good water solubility and biocompatibility, which can act as a template to prepare the Ag@CD-MOFs. These types of materials can achieve a dual function in reducing the particle size and enhancing the stability. Meanwhile, the small silver particles are easily dispersed in the aqueous media and exhibit an effective bacterial inhibition. The GRGDS peptide was modified on the surface of the Ag@ CD-MOFs to generate the MOF-33) (GS5-CL-Ag-@CD-MOFs) to enhance the hemostasis to promote the wound recovery and cooperate with the antibacterial effect [127]. The MOF-33 has an obvious antibacterial effect against E. coli. The MIC of E. coli is 16 µg mL −1 . The antibacterial mechanism of the MOF-33 is the synergistic effect of the CD-MOFs, Ag, and the GRGDS peptide, which means the smaller Ag particle is released from the Ag@ CD-MOF, which might reach immediate contact with the bacterial surface after the dissolution of the CD-MOF template that can increase their bactericidal activity. The peptide is beneficial to wound recovery (Figure 8) [127].
The MIC of E. coli is 16 µg mL −1 . The antibacterial mechanism of the MOF-33 is the synergistic effect of the CD-MOFs, Ag, and the GRGDS peptide, which means the smaller Ag particle is released from the Ag@ CD-MOF, which might reach immediate contact with the bacterial surface after the dissolution of the CD-MOF template that can increase their bactericidal activity. The peptide is beneficial to wound recovery ( Figure 8) [127].  The Mg-based MN-MOF-GO-Ag (MOF-34) was prepared, and the graphene oxidesilver nanocomposites (GO-Ag)-loaded poly (γ-glutamic acid) (γ-PGA) hydrogel was used as the backing layer of the microneedle (MN) patch. Meanwhile, the γ-PGA hydrogel loaded with the Mg-MOF was fabricated into the MN tips to achieve the controlled and long-termed release in the dermis. The Mg-MOFs have a lower cytotoxicity than the Cu. The gallic acid of MOF-34 can scavenge the overproduced intracellular reactive oxygen species. The GO-Ag can inhibit the foreign body accumulation and accelerate the healing process ( Figure 9) [128].
In Table 1, we have tabulated relevant data from the antibacterial activity with the silver-containing polymers @ MOFs 24-34. We have also explored their composition and the antibacterial mechanism of each MOF with other parameters. The silver or silvercontaining compounds are loaded into other MOFs to form silver-containing polymers that exploit better the antibacterial properties. The antibacterial mechanism associated with such MOFs is possibly due to the synergistic action of releasing the Ag + ions and compounds. The metallic silver ions are released from the frame system and adhere to the bacterial membrane; the Ag accumulation in the cell membrane affects its permeability [134].
The MOFs-24, 25, 27 and 29 exhibit a great antibacterial activity for the Gram-positive and Gram-negative bacterial strains, those compounds all use the MOFs as a repository, constantly releasing silver ions and the destruction of the framework and then penetrating the bacteria inside and the indirect production of the ROS by the Ag + . At the same time, these silver-containing polymers have an attractive antibacterial activity, due to the synergistic effect of the MOFs and the silver cations. In Table 1, we have tabulated relevant data from the antibacterial activity with th silver-containing polymers @ MOFs 24-34. We have also explored their composition an the antibacterial mechanism of each MOF with other parameters. The silver or silver-con taining compounds are loaded into other MOFs to form silver-containing polymers tha exploit better the antibacterial properties. The antibacterial mechanism associated wit such MOFs is possibly due to the synergistic action of releasing the Ag + ions and com pounds. The metallic silver ions are released from the frame system and adhere to th bacterial membrane; the Ag accumulation in the cell membrane affects its permeabilit [134].
The MOFs-24, 25, 27 and 29 exhibit a great antibacterial activity for the Gram-pos tive and Gram-negative bacterial strains, those compounds all use the MOFs as a repos tory, constantly releasing silver ions and the destruction of the framework and then pen etrating the bacteria inside and the indirect production of the ROS by the Ag + . At the sam time, these silver-containing polymers have an attractive antibacterial activity, due to th synergistic effect of the MOFs and the silver cations. The MOF-27 contains the Ni atom, which is a chelation reducing the polarity of the nickel ions. Moreover, the free organic moieties of the MOFs can bind with Ca(II) and Mg(II) of the microbial cells and the Ag ions, interacting with the thiol groups of proteins, inducing oxidative stress on the microbes and damaging the membrane [134,135]. The ZIF-8 and MIL-101 in MOFs-25 and 29 have a higher porosity, which is good for encapsulating silver and can release silver slowly to achieve the long-term antibacterial effect. The MIL-101 can control the particle size of the silver and make the silver ions fully dispersed, which is conducive to the greater antibacterial effect of the silver in the treatment process. The Zn 2+ ions can exert an antimicrobial activity against the bacterial strains, which is related to their ability to disrupt the cell membrane by the electrostatic interaction (zinc ions) or the generation of the reactive oxygen species (ROS) (zinc oxide), but also to bind to proteins and DNA, inactivating their functions and to modify the expression of several genes.
In the MOFs-25 and 26, other compounds also provide excellent conditions for the antibacterial effects, in addition to the zinc and silver metal ions. The HA possesses good hydrophilic and biodegradable properties, enhancing the bacterial-targeted drug release and reducing many side effects from the antibiotic drugs. The GO also plays a role in the antibacterial, synergistic effect of both the physical and chemical aspects. For example, the GO edge causes cell damage, which can directly interact with the cells and lead to the loss of integrity of the cell membrane; the production of the ROS and the transfer of charge; the dissociation of the oxygen-containing groups of the GO, which causes a decrease in the pH of the bacterial microenvironment.

Molecular Docking
Molecular docking can be used to predict the affinity and binding orientation of the drugs to their biotargets, such as proteins. In the article of Maryam Aghaee et al., the molecular docking experiment was used to prove that the Ag MOF has a certain effect on the antibacterial activity. The silver (I) metal-organic coordination polymer [Ag 2 L] n (H 2 L=1,4phenyl-enedipropionic acid) was fabricated using a sono-chemical technique [129]. [Ag 2 L] n is appropriately coordinated against the Gram-positive and Gram-negative bacteria, which means that it has an obvious antibacterial effect on both kinds of bacteria. The MIC of [Ag 2 L] n against E. coli and S. aureus, is ranged 0.06-0.125, 0.25 (ug/mL), respectively. The result of the molecular docking implies a favorable ligand-protein interaction energy at the binding cavity of the E. coli and S. aureus proteins. Both the Gram-positive and Gram-negative bacteria have potential ligand binding cavities, as shown in (Figure 10), which indicates that the active site in the MOF can realize the ligand-protein interaction in the ligand binding cavities of the two bacteria.  In the article by Azizolla Beheshti et al., the binding mode and the intermolecular interactions of the bbit and the cationic moiety of the asymmetric unit of the polymer {[Ag(u 2 -bbit)](BF 4 )} n (polymer 1) (bbit=1,1 -(butane-1,4-diyl)bis(3-methylimidazoline-2thione) ) with HSA (Human serum albumin), was investigated by the molecular docking method [135]. As shown in the ( Figure 11A), multiple C atoms in the bbit interact with multiple amino acids in the HSA by the hydrophobic interactions and the hydrogen bonding interactions, making the coordination polymer more stable. It is also indicated in Veysel T. Yilmaz's opinions, that the interaction between the complexes and HSA is mainly dominated by the hydrophobic and electrostatic interactions. From the docked structures presented in (Figure 11B), the Ag coordination polymers bind reasonably well to DNA through the partial intercalation favored in the grooves of the DNA rich in G/C bases [97]. In the article by Azizolla Beheshti et al., the binding mode and the intermolecular interactions of the bbit and the cationic moiety of the asymmetric unit of the polymer {[Ag(u2-bbit)](BF4)}n (polymer 1) (bbit=1,1′-(butane-1,4-diyl)bis(3-methylimidazoline-2thione) ) with HSA (Human serum albumin), was investigated by the molecular docking method [135]. As shown in the ( Figure 11A), multiple C atoms in the bbit interact with multiple amino acids in the HSA by the hydrophobic interactions and the hydrogen bonding interactions, making the coordination polymer more stable. It is also indicated in Veysel T. Yilmaz's opinions, that the interaction between the complexes and HSA is mainly dominated by the hydrophobic and electrostatic interactions. From the docked structures presented in (Figure 11B), the Ag coordination polymers bind reasonably well to DNA through the partial intercalation favored in the grooves of the DNA rich in G/C bases [97].  The molecular docking simulation experiment further shows that the antibacterial effect of the Ag-MOFs are not only to combine with the potential coordination cavity of the bacteria itself, but also to effectively combine with DNA to produce an interaction to achieve the best antibacterial effect. We can further study the specific antibacterial mechanism of the Ag MOF through the relevant ligand groups or active sites displayed by the molecular docking. As far as we know, antibacterial silver mainly refers to the high conductivity of the silver ions, which can attach to the cell membrane and damage the integrity of the membrane, causing the leakage of substances inside the membrane, DNA replication damage, etc.

Conclusions and Challenges
The most severe threat to the public's health is a pathogenic bacterial infection. At one time, the use of antibiotics allowed us to fight all kinds of bacterial infections effectively. However, the lack of understanding of antibiotics and people without knowing about the development of "bacterial resistance" led to the emergence of the current "superbugs". Over the last few decades, numerous efforts have been invested in solving these problems, and various antibacterial materials have been developed. In this perspective, we have focused on the antibacterial effect and mechanism of the Ag-MOFs and the Ag-containing polymer @MOF material as new anti-infective materials. The persistent antibacterial activity with an obvious antibacterial effect, a high stability, a low cost, and a low toxicity are the necessary characteristics of the Ag-MOFs in practical application.
On the antibacterial side, the Ag-MOFs express a superior antibacterial efficiency against the Gram-positive and Gram-negative bacteria strains, with a greater effect on