Experimental and Computational Analysis of Newly Synthesized Benzotriazinone Sulfonamides as Alpha-Glucosidase Inhibitors

Diabetes mellitus is a chronic metabolic disorder in which the pancreas secretes insulin but the body cells do not recognize it. As a result, carbohydrate metabolism causes hyperglycemia, which may be fatal for various organs. This disease is increasing day by day and it is prevalent among people of all ages, including young adults and children. Acarbose and miglitol are famous alpha-glucosidase inhibitors but they complicate patients with the problems of flatulence, pain, bloating, diarrhea, and loss of appetite. To overcome these challenges, it is crucial to discover new anti-diabetic drugs with minimal side effects. For this purpose, benzotriazinone sulfonamides were synthesized and their structures were characterized by FT-IR, 1H-NMR and 13C-NMR spectroscopy. In vitro alpha-glucosidase inhibition studies of all synthesized hybrids were conducted using the spectrophotometric method. The synthesized compounds revealed moderate-to-good inhibition activity; in particular, nitro derivatives 12e and 12f were found to be the most effective inhibitors against this enzyme, with IC50 values of 32.37 ± 0.15 µM and 37.75 ± 0.11 µM. In silico studies, including molecular docking as well as DFT analysis, also strengthened the experimental findings. Both leading compounds 12e and 12f showed strong hydrogen bonding interactions within the enzyme cavity. DFT studies also reinforced the strong binding interactions of these derivatives with biological molecules due to their lowest chemical hardness values and lowest orbital energy gap values.


Introduction
Diabetes mellitus is a metabolic disorder of the endocrine system characterized by hyperglycemia due to impaired insulin tolerance [1,2]. Insulin is the peptide hormone that maintains glucose homeostasis; it binds to cell receptors for the transfer of glucose molecules. This hormone also stimulates fat cells and muscles to obtain glucose from the blood and accelerates the liver for glucose metabolism until the sugar level is lowered In our efforts to develop an anti-diabetic drug with an enhanced therapeutic effect, we reported a series of benzotriazinone sulfonamide derivatives as potent alpha-glucosidase inhibitors, and one of them, compound 4 ( Figure 2), exhibited good results, with an IC50 value of 29.75 ± 0.14 μM [15]. In order to further enhance the potency of compound 4, our group designed another series of benzotriazinone sulfonamide derivatives (12a-f). This design mainly involved the replacement of the aryl group of compound 4 by alkyl In our efforts to develop an anti-diabetic drug with an enhanced therapeutic effect, we reported a series of benzotriazinone sulfonamide derivatives as potent alpha-glucosidase inhibitors, and one of them, compound 4 ( Figure 2), exhibited good results, with an IC 50 value of 29.75 ± 0.14 µM [15]. In order to further enhance the potency of compound 4, our group designed another series of benzotriazinone sulfonamide derivatives (12a-f). This design mainly involved the replacement of the aryl group of compound 4 by alkyl moiety at the third position, as well as the introduction of sulfonamide moiety at the sixth position of benzotriazinone ( Figure 2). Novel hybrids of 4-phenyl-N-(4-oxo-3-alkyl-3,4-dihydrobenzo[1,2,3]triazin-6-yl)benzenesulfonamide (12a-f) were prepared and their in vitro inhibition potential was studied in comparison to the commercial drug acarbose. Moreover, molecular docking analysis was employed to explain in vitro studies and to explore the binding mode of interactions of all hybrids against the binding sites of target alpha glucosidase. Furthermore, the stability and reactivity of the screened compounds were calculated by DFT studies to explore their hybrid interactions with other molecules. In our efforts to develop an anti-diabetic drug with an enhanced therapeutic effect, we reported a series of benzotriazinone sulfonamide derivatives as potent alpha-glucosidase inhibitors, and one of them, compound 4 ( Figure 2), exhibited good results, with an IC50 value of 29.75 ± 0.14 μM [15]. In order to further enhance the potency of compound 4, our group designed another series of benzotriazinone sulfonamide derivatives (12a-f). This design mainly involved the replacement of the aryl group of compound 4 by alkyl moiety at the third position, as well as the introduction of sulfonamide moiety at the sixth position of benzotriazinone ( Figure 2). Novel hybrids of 4-phenyl-N-(4-oxo-3-alkyl-3,4dihydrobenzo[1,2,3]triazin-6-yl)benzenesulfonamide (12a-f) were prepared and their in vitro inhibition potential was studied in comparison to the commercial drug acarbose. Moreover, molecular docking analysis was employed to explain in vitro studies and to explore the binding mode of interactions of all hybrids against the binding sites of target alpha glucosidase. Furthermore, the stability and reactivity of the screened compounds were calculated by DFT studies to explore their hybrid interactions with other molecules.

Spectroscopic Characterization
In multistep synthesis, the starting compounds 7, 8, 9a, 9b, 10a and 10b were prepared and characterized by FTIR spectral analysis, and observations were consistent with the values of similar compounds found in the literature [35,36]. The FTIR spectrum of isatoic anhydride 7 displayed strong absorption bands at 1722, 1765 cm −1 (C=O) and 1021 cm −1 (C-O-C), indicating the presence of anhydride moiety [35]. Regarding nitration, the presence of the NO 2 group in compound 8 was identified by FTIR band at 1355 cm −1 [36]. The anhydride group of compound 8 was reacted with alkyl amines, and as a result, N-alkyl anthranilamides 9a and 9b were formed as products, which were confirmed by the appearance of amino group IR bands at 3293-3318 cm −1 [37]. Further cyclization of anthranilamides was confirmed by the disappearance of NH 2 bands in both nitro benzotriazinones 10a and 10b [38]. Reduction of the NO 2 group to NH 2 was confirmed by the appearance of an IR band at 3430 cm −1 [39]. In sulfonamides (12a-12f), symmetrical stretching vibrational bands of S=O and NH were observed at 1157-1187 and 3185-3221 cm −1 [15]. Amino products 11a and 11b and all final products (12a-12f) were further characterized by 1 H-NMR and 13 C-NMR spectroscopic techniques. The amino group of 11a and 11b appeared at 6.56 ppm, while after conversion into the sulfonamide group, the NH group was noted to be in the range of 11.35-11.47 ppm. 1 H-NMR analysis of 11a, 11b and 12a-12f revealed the propyl protons as triplet, multiplet and triplet in the range of 0.84-4.82 ppm and the aromatic protons of phenyl rings were observed in the range of 7.02-8.12 ppm. Further 13 C-NMR spectral results confirmed the structure of all synthesized compounds.

Spectroscopic Characterization
In multistep synthesis, the starting compounds 7, 8, 9a, 9b, 10a and 10b were prepared and characterized by FTIR spectral analysis, and observations were consistent with the values of similar compounds found in the literature [35,36]. The FTIR spectrum of isatoic anhydride 7 displayed strong absorption bands at 1722, 1765 cm −1 (C=O) and 1021 cm −1 (C-O-C), indicating the presence of anhydride moiety [35]. Regarding nitration, the presence of the NO2 group in compound 8 was identified by FTIR band at 1355 cm −1 [36]. The anhydride group of compound 8 was reacted with alkyl amines, and as a result, Nalkyl anthranilamides 9a and 9b were formed as products, which were confirmed by the appearance of amino group IR bands at 3293-3318 cm −1 [37]. Further cyclization of anthranilamides was confirmed by the disappearance of NH2 bands in both nitro benzotriazinones 10a and 10b [38]. Reduction of the NO2 group to NH2 was confirmed by the appearance of an IR band at 3430 cm −1 [39]. In sulfonamides (12a-12f), symmetrical stretching Scheme 1. Synthetic route for the synthesis of 1,2,3-benzotriazin-4(3H)-one based sulfonamides.

Alpha-Glucosidase Inhibition Studies
Acarbose is a commercially available antidiabetic drug used to treat diabetes mellitus. It actively works in the intestinal tract by inhibiting alpha-glucosidase, which is responsible for the conversion of complex carbohydrates to absorbable glucose. Acarbose has various side effects and chemists are working to discover a new effective anti-diabetic drug. Therefore, an alpha-glucosidase inhibition assay of newly synthesized amino benzotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC 50 value of 32.37 ± 0.15 µM as compared to the standard drug acarbose, with an IC 50 value of 37.38 ± 0.12 µM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC 50 value of 37.75 ± 0.11 µM.
The difference in the inhibition potentials of 12e and 12f illustrated that the small chain of the propyl group is more appropriate to be fitted with an alpha-glucosidase structure. Electron density on oxygen atoms of the nitro group enabled it to have a strong inhibitory potential. Different groups at the fourth position of the phenyl ring revealed different inhibition strengths. Compound 12a indicated a lesser enzyme inhibition potency as compared to other compounds having Cl, Br, NO 2 and OCH 3 groups. The lone pair containing substituents on the phenyl ring supported inhibition activity due to their electronic interactions with enzyme (active) sites. The larger size of the bromine atom reduced the inhibition activity of compound 12d (IC 50 value 55.21 ± 0.12 µM) as compared to the chloro-containing compound 12c (IC 50 value 53.27 ± 0.13 µM). The methoxy derivative 12b was found to be more effective inhibitor as compared to 12a but less active than 12e and 12f. zotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. zotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. zotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. zotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. drug. Therefore, an alpha-glucosidase inhibition assay of newly synthesized amino benzotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. drug. Therefore, an alpha-glucosidase inhibition assay of newly synthesized amino benzotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. drug. Therefore, an alpha-glucosidase inhibition assay of newly synthesized amino benzotriazinones 11a-11b and their corresponding sulfonamides 12a-12f was carried out by spectrophotometric method. Comparative results of all compounds (12a-12f) with reference to standard drug acarbose are given in Table 1. The results proved to have fair to good inhibition activity. From Table 1, it is evident that amino benzotriazinones 11a and 11b exhibited less inhibition in contrast to their sulfonamides, which indicated the effective interactions of sulfonyl group within the enzyme cavity. Nitro derivative 12e revealed the most promising activity, with an IC50 value of 32.37 ± 0.15 μM as compared to the standard drug acarbose, with an IC50 value of 37.38 ± 0.12 μM. Another nitro compound (12f) with the butyl group was found to be an equivalent inhibitor of acarbose, with an IC50 value of 37.75 ± 0.11 μM. The difference in the inhibition potentials of 12e and 12f illustrated that the small chain of the propyl group is more appropriate to be fitted with an alpha-glucosidase structure. Electron density on oxygen atoms of the nitro group enabled it to have a strong inhibitory potential. Different groups at the fourth position of the phenyl ring revealed different inhibition strengths. Compound 12a indicated a lesser enzyme inhibition potency as compared to other compounds having Cl, Br, NO2 and OCH3 groups. The lone pair containing substituents on the phenyl ring supported inhibition activity due to their electronic interactions with enzyme (active) sites. The larger size of the bromine atom reduced the inhibition activity of compound 12d (IC50 value 55.21 ± 0.12 μM) as compared to the chloro-containing compound 12c (IC50 value 53.27 ± 0.13 μM). The methoxy derivative 12b was found to be more effective inhibitor as compared to 12a but less active than 12e and 12f.

Molecular Docking
The probable binding interactions of the tested compounds within the enzyme cavity were explored by molecular docking studies. Autodoc Vina and its tools were utilized to elaborate all types of interactions. The receptor file of alpha-glucosidase was prepared by homology modeling, as reported in our previous work [15]. Chemdraw office pack was utilized to prepare and optimize the ligand's structure by using the MM2 force field.
Both 12e and 12f formed stable ligand enzyme complexes and displayed the highest binding energy, −9.5 and −9.3 kcal.mol −1 , respectively. Binding pose of the docked conformation with highest binding affinity of both ligands 12e and 12f are presented in Figures

Molecular Docking
The probable binding interactions of the tested compounds within the enzyme cavity were explored by molecular docking studies. Autodoc Vina and its tools were utilized to elaborate all types of interactions. The receptor file of alpha-glucosidase was prepared by homology modeling, as reported in our previous work [15]. Chemdraw office pack was utilized to prepare and optimize the ligand's structure by using the MM2 force field.
Both 12e and 12f formed stable ligand enzyme complexes and displayed the highest binding energy, −9.5 and −9.3 kcal.mol −1 , respectively. Binding pose of the docked conformation with highest binding affinity of both ligands 12e and 12f are presented in Figures 3 and 4, respectively. From these figures, it is evident that both ligands are fit inside the enzyme pockets due to different kinds of binding interactions, including electrostatic, hydrogen bonding as well as hydrophobic interactions. In these two cases, the highest binding may be associated with the development of hydrogen bonding between the carbonyl group of ligands and the carboxylic group of GLU276. The nitro group of 12e and 12f also established hydrogen bonding with the NH group of HIS279. Similarly, one conventional hydrogen bond can be seen between nitrogen of the benzotriazinone ring and the NH group of the ARG439 amino acid (Figures 3 and 4).   All rings, along with alkyl groups of both ligands 12e and 12f, established various kinds of hydrophobic interactions with the residues of different amino acids (Figures 3  and 4). Significantly, pi-pi T-shaped interactions appeared between pi-electron densities of both ligands and aromatic side chains of PHE157 and HIS279. Similarly, pi-alkyl interactions between the methyl group of ALA278 and the ring density of the benzene ring attached with the nitro group can be observed in the case of both ligands. All mentioned forces made strong ligand enzyme complexes that enabled these compounds (12e and 12f) to act as effective alpha-glucosidase inhibitors. Molecular docking results explored that both 12e and 12f docked compounds have almost similar kinds of interactions within the active pockets of enzyme (Figures 3 and 4). However, the relatively lesser activity of compound 12f as compared to 12e can be explained in terms of increasing hydrophobicity of   All rings, along with alkyl groups of both ligands 12e and 12f, established various kinds of hydrophobic interactions with the residues of different amino acids (Figures 3  and 4). Significantly, pi-pi T-shaped interactions appeared between pi-electron densities of both ligands and aromatic side chains of PHE157 and HIS279. Similarly, pi-alkyl interactions between the methyl group of ALA278 and the ring density of the benzene ring attached with the nitro group can be observed in the case of both ligands. All mentioned forces made strong ligand enzyme complexes that enabled these compounds (12e and 12f) to act as effective alpha-glucosidase inhibitors. Molecular docking results explored that both 12e and 12f docked compounds have almost similar kinds of interactions within the active pockets of enzyme (Figures 3 and 4). However, the relatively lesser activity of compound 12f as compared to 12e can be explained in terms of increasing hydrophobicity of Similarly, the benzotriazinone ring of both ligands (12e and 12f) depicted pi-anionic electrostatic interaction with the carboxylic acid group of ASP349. However, an additional pi-cationic interaction between the NH group of ARG349 and the benzotriazinone ring was found in the case of compound 12e (Figures 3 and 4).
All rings, along with alkyl groups of both ligands 12e and 12f, established various kinds of hydrophobic interactions with the residues of different amino acids (Figures 3 and 4). Significantly, pi-pi T-shaped interactions appeared between pi-electron densities of both ligands and aromatic side chains of PHE157 and HIS279. Similarly, pi-alkyl interactions between the methyl group of ALA278 and the ring density of the benzene ring attached with the nitro group can be observed in the case of both ligands. All mentioned forces made strong ligand enzyme complexes that enabled these compounds (12e and 12f) to act as effective alpha-glucosidase inhibitors. Molecular docking results explored that both 12e and 12f docked compounds have almost similar kinds of interactions within the active pockets of enzyme (Figures 3 and 4). However, the relatively lesser activity of compound 12f as compared to 12e can be explained in terms of increasing hydrophobicity of the ligand due to the increasing chain of the alkyl group in compound 12f that makes the ligand-enzyme complex relatively less stable, as observed in the binding energies.

Frontier Molecular Orbital Analysis
Quantum chemistry calculations regarding the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) are collectively known as frontier molecular orbital analyses (FMO). FMO analyses are helpful in drug design and in determining reactivity. HOMO is the highest energy orbital of molecule with electrons while LUMO is the lowest energy orbital of molecule with no electrons [40]. Energy difference between HOMO and LUMO is the main factor for compound reactivity. All synthesized compounds 12a-12f were studied by frontier molecular orbital analysis and results supported that all synthesized molecules have high chemical reactivity [41,42].
Energy of HOMO (E HOMO ), LUMO (E LUMO ) and energy gaps (E gap = HOMO-LUMO) between them were calculated by B3LYP/6-311+G* level of DFT method ( Table 2). Large energy gap value illustrates that compound is less reactive while small energy gap indicates the less kinetic stability and high chemical reactivity. Among all compounds, 12e and 12f were found to have lowest energy gap values revealing that these compounds are more reactive as compared to others. Furthermore, the HOMO-LUMO energy analysis of all compounds provided valuable information about their global reactivity descriptors like E HOMO , E LUMO , electrophilicity, chemical potential, chemical softness and various other electronic parameters which are tabulated in Table 2 [40,43]. The electrophilicity values (ω) of all compounds explained their bonding, stability, structure and reactivity [40,43]. Compounds 12e and 12f presented the highest values of electrophilicity, 7.25 and 7.26 eV, which is evidence of their greater interactions with biomolecules. Electrophilicity index with molecular docking studies supported the in vitro alpha-glucosidase inhibition results where 12e and 12f exhibited good inhibitory activity.
For the synthesized series 12a-12f, delocalization of both HOMO and LUMO was identified by charge density, as demonstrated in Figure 5. It was observed that HOMO is located on the site of the benzotriazinone ring, and sulfonamide moiety and LUMO delocalization is different in different molecules. The compound with no substitution, 12a, as well as the compound with an electron donating group, 12b, exhibited their LUMO delocalizations confined on the benzotriazinone ring. While in compounds 12c and 12d, the electron density of LUMO was delocalized on their phenyl ring and sulfonamide moiety. However, 12a, 12b and 12d have LUMO delocalization on the surface of the benzotriazinone ring and sulfonamide group ( Figure 5). The compounds 12c, 12d, 12e and 12f have electron withdrawing groups (Cl, Br and NO 2 ), restraining LUMO electron density only on the phenyl ring.

Molecular Electrostatic Potential
At the molecular level, MEP is an effective method to describe the electron density distribution map inside molecules and can illustrate an idea about the reactive sites of molecular structure. This method provides images according to the relative polarity present in a molecule, which indicate the electrophilic portion (negative site) and nucleophilic portion (positive side) of the compound. MEP studies were carried out using a B3LYP/6-

Molecular Electrostatic Potential
At the molecular level, MEP is an effective method to describe the electron density distribution map inside molecules and can illustrate an idea about the reactive sites of molecular structure. This method provides images according to the relative polarity present in a molecule, which indicate the electrophilic portion (negative site) and nucleophilic portion (positive side) of the compound. MEP studies were carried out using a B3LYP/6-311+G* method and the results are shown in Figure 6. Surface maps displayed pictures with different colors, indicating different electrostatic potential values. The positive sites are shown by the blue colour, while the yellow colour depicts slightly positive areas, and neutral electrostatic potential regions are represented by green. The positive sites indicated by the blue colour of all molecules tend to repel protons. Further, this study helped to identify reactive sites of chemical systems and indicated the sites where ligand can bind to enzyme. MEP analysis of all compounds depicted the existence of strong positive potential on hydrogen atoms of sulfonamide functionality and partial positive charge on C=O, N=N and SO 2 groups. The existence of strong and partial positive charges on functional groups of synthesized series have a tendency to develop appropriate interactions within the enzyme cavity.
Molecules 2022, 27, x FOR PEER REVIEW 10 of 17 311+G* method and the results are shown in Figure 6. Surface maps displayed pictures with different colors, indicating different electrostatic potential values. The positive sites are shown by the blue colour, while the yellow colour depicts slightly positive areas, and neutral electrostatic potential regions are represented by green. The positive sites indicated by the blue colour of all molecules tend to repel protons. Further, this study helped to identify reactive sites of chemical systems and indicated the sites where ligand can bind to enzyme. MEP analysis of all compounds depicted the existence of strong positive potential on hydrogen atoms of sulfonamide functionality and partial positive charge on C=O, N=N and SO2 groups. The existence of strong and partial positive charges on functional groups of synthesized series have a tendency to develop appropriate interactions within the enzyme cavity.

General
All chemicals were purchased from international supplier, Sigma-Aldrich (USA and Germany), Fluka (Switzerland). Merck TLC plates (Germany) were used to monitor the progress of reactions under short and long wavelengths (model UVGL-25 minor light multiband UV-254/366). VWR formic acid (USA) and Merck glacial acetic acid (Germany) were used in the synthesis of isatoic anhydride. Melting points were recorded on the Fisher-Johns apparatus (University of the Punjab, Lahore), calibrated by benzoic acid. The FTIR spectra were taken on Agilent 630 FTIR (University of the Punjab, Lahore). In NMR (H.E.J Research institute of chemistry, University of Karachi), TMS was used as internal standard and all spectra were recorded in CDCl3 or DMSO-d6 on Brucker (300, 400 and 500 MHz). (7) Isatin (6, 14.7 g, 0.1 mmol) and formic acid (80 mL) were taken in an Erlenmeyer flask. Hydrogen peroxide (30%, 20 mL) was added slowly with slight cooling, and the reaction mixture was stirred for 1h at room temperature. Precipitates of product appeared in the flask and were filtered and recrystallized from methanol. Light yellow compound; yield: (13.

General
All chemicals were purchased from international supplier, Sigma-Aldrich (USA and Germany), Fluka (Switzerland). Merck TLC plates (Germany) were used to monitor the progress of reactions under short and long wavelengths (model UVGL-25 minor light multiband UV-254/366). VWR formic acid (USA) and Merck glacial acetic acid (Germany) were used in the synthesis of isatoic anhydride. Melting points were recorded on the Fisher-Johns apparatus (University of the Punjab, Lahore), calibrated by benzoic acid. The FTIR spectra were taken on Agilent 630 FTIR (University of the Punjab, Lahore). In NMR (H.E.J Research institute of chemistry, University of Karachi), TMS was used as internal standard and all spectra were recorded in CDCl 3 or DMSO-d 6 on Brucker (300, 400 and 500 MHz). (7) Isatin (6, 14.7 g, 0.1 mmol) and formic acid (80 mL) were taken in an Erlenmeyer flask. Hydrogen peroxide (30%, 20 mL) was added slowly with slight cooling, and the reaction mixture was stirred for 1h at room temperature. Precipitates of product appeared in the flask and were filtered and recrystallized from methanol. Light yellow compound; yield: (13. In a conical flask, compound 9a/9b (3 g, 12.8 mmol) was taken in chilled water (20 mL), and HCl solution (30%, 5 mL) was added into it. The reaction mixture was placed in an ice bath to maintain the temperature at 0-5 • C. Sodium nitrite (16.6 mmol) was dissolved in distilled water (10 mL) and this solution was slowly added to the reaction mixture; the temperature was maintained below 5 • C. Afterwards, the reaction was stirred for two hours and ice-cold water was added to obtain precipitates of product. The product was washed twice with chilled water and recrystallized from methanol followed by drying at 70 • C. Compound 10a-10b (0.967 mmol), iron powder (2.42 g, 10 mmol) and HCl (30%, 5 mL) solution were taken in water (10 mL). Reaction mixture was stirred for two hours at 60 • C. Afterwards, the reaction mixture was filtered and the filtrate was cooled until crystals of product appeared. It was then recrystallized with chloroform. .0 (C, NCH 2 CH 2 CH 2 CH 3 ), 19.8 (C, NCH 2 CH 2 CH 2 CH 3 ), 31.0 (C, NCH 2 CH 2 CH 2 CH 3 ), 48.7 (C, NCH 2 CH 2 CH 2 CH 3 ), 103.4 (CH, C-5), 121.9 (CH, C-7), 122.3 (C, C-4a), 130.4 (CH, C-8), 136.2 (C, C-8a), 153.6 (C, C-6), 155.6 (CO, C-4).

Synthesis of Isatoic Anhydride
3.1.6. General Procedure for the Synthesis of N-(4-Oxo-3-propyl/butyl-3,4-dihydrobenzo [1,2,3]triazin-6-yl)benzenesulfonamides (12a-12f) Compound 11a/11b (0.1 g, 0.49 mmol), benzene sulfonyl chloride (0.49 mmol) and pyridine (10 mL) were taken in a 50 mL round bottom flask and refluxed for 5 h. Then, the pyridine was evaporated under reduced pressure and the mixture was cooled to room temperature. A few drops of dilute HCl were added to ice-cold water (100 mL) until the pH reached 3, and the temperature was maintained at 5 • C. Further, the reaction mixture was poured off into acidified water. Precipitates appearing in the solution were filtered, dried and recrystallized from methanol. The physiochemical properties of all synthesized compounds are given in Table 3.

Procedure of Alpha-Glucosidase Inhibition Assay
Alpha-glucosidase inhibition activity was carried out by the Pierre et al. method with slight modifications, and alpha-Glucosidase (Cat No. 5003-1KU Type I) from Saccharomyces cereviciae was utilized for the experimental protocol due to its resemblances to the structure and function of yeast/mammalian enzyme. For test, saline phosphate buffer (70 µL, 50 mM with pH 6.8), alpha-glucosidase enzyme (10 µL, 0.0234 units) and test compound (10 µL, 0.5 mM) were taken in test tubes and incubated for 10 min at 37 • C. Absorbance of these prepared samples was recorded at 400 nm. 10 µL p-nitrophenyl-alpha-D-glucopyranoside (0.5 mM, 'substrate', code No. N1377 from Sigma) was used to initiate the reaction and test tubes were allowed to stand for 30 min. Then, the variation in concentration of free substrate was noted by the change in absorbance. The enzyme percentage inhibition was estimated by the following formula and IC 50 values were calculated by 'EZ-Fit enzyme kinetics software. % Inhibition = [(Abs. of test − Abs. of control)/Abs. of control] × 100

Conclusions
A series of 1,2,3-benzotriazin-4(3H)-one based sulfonamides was designed, synthesized and discovered to be effective alpha-glucosidase inhibitors. Among all other derivatives, compound 12e (IC 50 32.37 ± 0.15 µM) was found to be a better inhibitor as compared to the standard drug acarbose (IC 50 37.38 ± 0.12 µM). Moreover, the inhibition potential of hybrid 12f (IC 50 37.75 ± 0.11 µM) was observed to be comparable to the reference drug. The enzyme inhibition mechanism was unlocked by molecular docking studies and significant interactions between the leading ligands and the targeted receptor were explored. In DFT, frontier molecular orbital analysis and molecular electrostatic potential studies were employed to understand various electronic parameters of all synthesized compounds. The least energy gap (E gap ) value between HOMO and LUMO depicted the reactive nature of 12e and 12f. In addition, the higher electrophilicity value of these compounds also acknowledged their interactive nature towards biomolecules. The electrophilic and nucleophilic sites of all synthesized compounds were shown by MEP studies. Hence, the in vitro alpha-glucosidase inhibitory results were in good agreement with the outcomes of molecular docking and the performed DFT calculations.