Bioactive Diarylpentanoids: Insights into the Biological Effects beyond Antitumor Activity and Structure–Activity Relationships

Diarylpentanoids, a class of natural products and their synthetic analogs which are structurally related to chalcones, have gained increasing attention due to their wide array of biological activities, including antitumor, anti-infective, antioxidant, anti-inflammatory, antidiabetic, anti-hyperuricemic, and neuroprotective properties. Previously, we reviewed diarylpentanoids with promising antitumor activity. However, in view of the wide range of biological activities described for this class of compounds, the purpose of this review is to provide a more detailed overview of the synthetic bioactive diarylpentanoids that have been described over the last two decades, beyond simply their antitumor effects. A total of 745 compounds were found, highlighting the main synthetic methodologies used in their synthesis as well as the structure–activity relationship studies and structural features for all activities reported. Collectively, this review highlights the diarylpentanoid scaffold as a promising starting point for the development of new therapeutic agents.


Introduction
Diarylpentanoids comprise a class of natural products and their synthetic analogs possessing two aromatic rings, which may or may not have the same substitution pattern, which are linked by a five-carbon bridge. Most of these compounds possess an acyclic or cyclic C5 bridge with a dienone moiety linking the two aromatic rings, with the presence of symmetric or asymmetric linkers also being reported, as highlighted in Figure 1. These compounds show a wide array of biological activities, including antioxidant, anti-inflammatory, antitumor, and antibacterial properties among others.
The presence of an enone moiety between the two aromatic rings in the majority of these compounds makes them structurally related to chalcones, a well-known class of natural products with a wide range of biological activities [1][2][3][4]. Diarylpentanoids are also known as C5-curcuminoids since these are structurally related to curcumin (1E,6E -17-bis(4hydroxy-3-methoxyphenyl)hepta-16-diene-35-dione (1: Figure 2), a C7-curcuminoid found in the traditional Indian herb Curcuma Longa [5]. This natural product (1) has shown diverse biological activities, such as antioxidant, antidiabetic, antimicrobial, antimalarial, antiviral, thrombosuppressive, antitumor, cardio-and neuro-protective properties, as well as chemopreventive activity [6][7][8][9][10] associated with different targets, which supports the idea that this compound influences a great variety of biochemical and molecular cascades [11]. However, curcumin (1: Figure 2) displays some limitations, such as chemical instability, poor aqueous The presence of an enone moiety between the two aromatic rings in the majority of these compounds makes them structurally related to chalcones, a well-known class of natural products with a wide range of biological activities [1][2][3][4]. Diarylpentanoids are also known as C5-curcuminoids since these are structurally related to curcumin (1E,6E -17bis(4-hydroxy-3-methoxyphenyl)hepta-16-diene-35-dione (1: Figure 2), a C7-curcuminoid found in the traditional Indian herb Curcuma Longa [5]. This natural product (1) has shown diverse biological activities, such as antioxidant, antidiabetic, antimicrobial, antimalarial, antiviral, thrombosuppressive, antitumor, cardio-and neuro-protective properties, as well as chemopreventive activity [6][7][8][9][10] associated with different targets, which supports the idea that this compound influences a great variety of biochemical and molecular cascades [11]. However, curcumin (1: Figure 2) displays some limitations, such as chemical instability, poor aqueous solubility, low bioavailability, and fast metabolism under physiological conditions. These limitations have led to the development of several studies into synthetic curcumin analogs. Among these studies, the synthesis of novel curcumin analogs by molecular modifications, namely of the diketo group, was previously described [11]. The 1,3-dicarbonyl moiety is affected by in vitro and in vivo degradation through pathways including oxidation and hydrolysis. Suppression of the delocalization of electrons on the structure has been shown to improve curcuminoid stability and subsequent bioavailability [12]. On the other hand, the double bonds present on the linker bridge, although recognized as important for activity, are considered as part of the linker between the two essential structural elements and have not been widely modified [13]. In this scope, diarylpentanoids arose as an alternative.  The presence of an enone moiety between the two aromatic rings in the majority of these compounds makes them structurally related to chalcones, a well-known class of natural products with a wide range of biological activities [1][2][3][4]. Diarylpentanoids are also known as C5-curcuminoids since these are structurally related to curcumin (1E,6E -17bis(4-hydroxy-3-methoxyphenyl)hepta-16-diene-35-dione (1: Figure 2), a C7-curcuminoid found in the traditional Indian herb Curcuma Longa [5]. This natural product (1) has shown diverse biological activities, such as antioxidant, antidiabetic, antimicrobial, antimalarial, antiviral, thrombosuppressive, antitumor, cardio-and neuro-protective properties, as well as chemopreventive activity [6][7][8][9][10] associated with different targets, which supports the idea that this compound influences a great variety of biochemical and molecular cascades [11]. However, curcumin (1: Figure 2) displays some limitations, such as chemical instability, poor aqueous solubility, low bioavailability, and fast metabolism under physiological conditions. These limitations have led to the development of several studies into synthetic curcumin analogs. Among these studies, the synthesis of novel curcumin analogs by molecular modifications, namely of the diketo group, was previously described [11]. The 1,3-dicarbonyl moiety is affected by in vitro and in vivo degradation through pathways including oxidation and hydrolysis. Suppression of the delocalization of electrons on the structure has been shown to improve curcuminoid stability and subsequent bioavailability [12]. On the other hand, the double bonds present on the linker bridge, although recognized as important for activity, are considered as part of the linker between the two essential structural elements and have not been widely modified [13]. In this scope, diarylpentanoids arose as an alternative. Following our interest in this class of compounds [14][15][16][17], we previously reviewed diarylpentanoids with promising antitumor activity [18]. Nevertheless, considering the wide range of biological activities described for this class of compounds, and considering the inexistence of exhaustive review articles focusing on other biological activities of Following our interest in this class of compounds [14][15][16][17], we previously reviewed diarylpentanoids with promising antitumor activity [18]. Nevertheless, considering the wide range of biological activities described for this class of compounds, and considering the inexistence of exhaustive review articles focusing on other biological activities of diarylpentanoids, this study is intended to review these diverse biological effects beyond the reported antitumor activity. Moreover, some insights about the synthetic methods used to obtain these compounds, as well as the mechanisms involved in these activities and the putative structural features relevant for the respective biological activities, are provided. Data concerning synthesis and structure-activity relationship studies (SAR) of diarylpentanoids, organized by biological activity and by chronological order of the year in which they were first reported, are presented in the next sections and in Table S1 (Supplementary Materials).

Synthesis of the Diarylpentanoids
The most employed method for the synthesis of diarylpentanoids is Claisen-Schmidt condensation between a suitable ketone and a substituted benzaldehyde in the presence of a polar solvent while using a strong base or acid (Scheme 1). For example, Du et al. (2006) synthesized symmetric diarylpentanoids by coupling the substituted aromatic aldehyde with ketone under acidic conditions and used a ratio of ketone:aldehyde of 1:2 at 25-30 • C [19]. Lee et al. (2009) synthesized a series of symmetric diarylpentanoids in the presence of NaOH in ethanol [20]. diarylpentanoids, organized by biological activity and by chronological order of the year in which they were first reported, are presented in the next sections and in Table S1 (Supplementary Materials).

Synthesis of the Diarylpentanoids
The most employed method for the synthesis of diarylpentanoids is Claisen-Schmidt condensation between a suitable ketone and a substituted benzaldehyde in the presence of a polar solvent while using a strong base or acid (Scheme 1). For example, Du et al. (2006) synthesized symmetric diarylpentanoids by coupling the substituted aromatic aldehyde with ketone under acidic conditions and used a ratio of ketone:aldehyde of 1:2 at 25-30 °C [19]. Lee et al. (2009) synthesized a series of symmetric diarylpentanoids in the presence of NaOH in ethanol [20]. Scheme 1. General synthesis of symmetric diarylpentanoids. The ketone could be acetone (n = 0), cyclopentanone (n = 2), or cyclohexanone (n = 3). Reaction conditions (i): NaOH or HCl.
However, to obtain asymmetric compounds with different aryl rings, a monoaryl intermediate should be firstly prepared by Stork reaction and subsequently condensed with the appropriately substituted aldehyde to give the target compounds (Scheme 2A). When asymmetry is present in the C5 bridge, different reaction conditions are used depending on the C5 bridge. In the case of diarylpentanoids with an α-substituted enone moiety, the intermediates are first prepared by condensation using HCl as a catalyst, and the intermediate is then treated with substituted benzaldehyde in the presence of bases (Scheme 2B) [21]. Faudzi et al. (2015) performed the synthesis of diarylpentanoids, with the 1-oxo-pent-2,4-diene moiety as an acyclic C5 bridge, through the reaction of an appropriately substituted cinnamaldehyde and a ketone under alkaline/ethanol conditions (Scheme 2C) [22]. However, to obtain asymmetric compounds with different aryl rings, a monoaryl intermediate should be firstly prepared by Stork reaction and subsequently condensed with the appropriately substituted aldehyde to give the target compounds (Scheme 2A). When asymmetry is present in the C5 bridge, different reaction conditions are used depending on the C5 bridge. In the case of diarylpentanoids with an α-substituted enone moiety, the intermediates are first prepared by condensation using HCl as a catalyst, and the intermediate is then treated with substituted benzaldehyde in the presence of bases (Scheme 2B) [21]. Faudzi et al. (2015) performed the synthesis of diarylpentanoids, with the 1-oxo-pent-2,4diene moiety as an acyclic C5 bridge, through the reaction of an appropriately substituted cinnamaldehyde and a ketone under alkaline/ethanol conditions (Scheme 2C) [22].

Synthesis of the Diarylpentanoids
The most employed method for the synthesis of diarylpentanoids is Claisen-Schmidt condensation between a suitable ketone and a substituted benzaldehyde in the presence of a polar solvent while using a strong base or acid (Scheme 1). For example, Du et al. (2006) synthesized symmetric diarylpentanoids by coupling the substituted aromatic aldehyde with ketone under acidic conditions and used a ratio of ketone:aldehyde of 1:2 at 25-30 °C [19]. Lee et al. (2009) synthesized a series of symmetric diarylpentanoids in the presence of NaOH in ethanol [20]. Scheme 1. General synthesis of symmetric diarylpentanoids. The ketone could be acetone (n = 0), cyclopentanone (n = 2), or cyclohexanone (n = 3). Reaction conditions (i): NaOH or HCl.
However, to obtain asymmetric compounds with different aryl rings, a monoaryl intermediate should be firstly prepared by Stork reaction and subsequently condensed with the appropriately substituted aldehyde to give the target compounds (Scheme 2A). When asymmetry is present in the C5 bridge, different reaction conditions are used depending on the C5 bridge. In the case of diarylpentanoids with an α-substituted enone moiety, the intermediates are first prepared by condensation using HCl as a catalyst, and the intermediate is then treated with substituted benzaldehyde in the presence of bases (Scheme 2B) [21]. Faudzi et al. (2015) performed the synthesis of diarylpentanoids, with the 1-oxo-pent-2,4-diene moiety as an acyclic C5 bridge, through the reaction of an appropriately substituted cinnamaldehyde and a ketone under alkaline/ethanol conditions (Scheme 2C) [22].

Anti-Infective Activity
Infectious diseases caused by pathogens, such as bacteria, fungi, and viruses, are major challenges for humankind as multidrug resistance to antibiotics, antivirals, and antiparasitic agents is one of the major causes of the alarming level of infectious diseases worldwide [23]. The discovery of new drugs with potent anti-infective activity, particularly against the resistant strains, is therefore highly desirable. Diarylpentanoids have been reported as a promising class of compounds with anti-infective activity, namely antibacterial and antiparasitic activity.  Figure 3) and with a C5 bridge embodied in a cyclic moiety, namely cyclopentanone (12-26: Figure 3 A origem da referência não foi encontrada.) and cyclohexanone (27-36: Figure 3). Their in vitro antibacterial activity against seven Gram-positive and Gram-negative bacteria were tested by determining the zone of inhibition. Compounds 8, 9, 11, 21, and 36 (inhibition zone (IZ) = 9-20 mm) showed remarkable in vitro antibacterial activity. It was observed that some of the derivatives displayed significant activity when compared to curcumin (1), and most of them exhibited activity against ampicillin-resistant E. cloacae. These results allow for some structureactivity relationship (SAR) considerations, as highlighted in Figure 4. It was shown that the presence of heterocyclic aryl rings (11, 26, and 36) was associated with an enhancement of activity, with diarylpentanoids with a C5 acyclic bridge (11) and with a cyclohexanone moiety (36) being more potent than those with a cyclopentanone moiety (26) (Figure 4). The comparison of biological data obtained for diarylpentanoids with phenyl rings reinforces that the C5 bridge can have some influence on antibacterial activity ( Figure 4). Additionally, a long-chain amino substituent in compound 21 may play an important role in bioactivity ( Figure 4) [24].
In 2022, our group reported the synthesis of a library of symmetric diarylpentanoids (51-71: Figure 3) and investigated their potential as antimicrobial agents and as reversers of antimicrobial resistance [17]. The compounds were investigated for their antimicrobial activity in susceptible and resistant bacterial and fungal strains. Although none of the tested compounds showed activity against the used bacterial and fungal strains (MIC > 100 µM or MIC > 64 µg/mL for bacteria and MIC > 128 µg/mL for fungi), most of the compounds displayed promising results in combination with antibacterial drugs, with diarylpentanoid 63 being the most promising. Considering the results obtained, some fundamental SAR guidelines were established ( Figure 4). For E. coli SA/2, diarylpentanoids with a single para-substituent with an electron-withdrawing effect in the B phenyl group (59, 60, and 62) were more potent than the ortho-substituted analogs (51)(52)(53). For compounds with di-or trisubstituted phenyl rings, substitution at the orthoor para-position appeared to be crucial for activity (63-66 and 68) since, when none of these positions was substituted, non-active compounds were obtained (67). Comparing the effects of diarylpentanoids with the same aryl rings and different C5 bridges, it seems that the presence of a linker with a tetrahydro-4H-pyran-4-one moiety (52-54, 58-66, and 68) was much more favorable for a positive effect in terms of cefotaxime activity than cyclohexanone (69), tetrahydro-4H-thiopyran-4-one (70), and cyclopentanone (71) moieties. Curiously, for E. faecalis, the presence of a linker with a cyclohexanone (69), tetrahydro-4H-thiopyran-4-one (70), or cyclopentanone (71) moiety was more favorable than a linker with a tetrahydro-4H-pyran-4-one (52-54, 59, 60, 62, 64-66, and 68) moiety [17].
The compounds that displayed promising results were also evaluated for their activity in efflux pump-related virulence mechanisms, such as biofilm formation and quorumsensing. The set of compounds 59, 63, 66, and 69 ( Figure 3) was considered to be active toward the inhibition of efflux pumps, mainly in Staphylococcus aureus 272123. Compound 63 inhibited biofilm formation by S. aureus 272123 and by Sphingomonas paucimobilis Ezf 10-17 and Chromobacterium violaceum CV026, while compound 69 only inhibited biofilm formation by S. aureus 272123 [17].

Antiparasitic Activity
Leishmaniasis is a group of prevalent diseases caused by protozoan parasites of the genus Leishmania. Alves et al. (2003) reported the in vitro antileishmanial activity of a new series of asymmetric diarylpentanoids (72-78: Figure 5) against L. amazonensis, L. braziliensis, and L. chagasi promastigotes. It was observed that diarylpentanoids were more effective against L. braziliensis promastigotes (IC 50 values between 23 and 78 µM) than against L. amazonensis and L. chagasi (IC 50 values ranging from 3 to 373 µM and 6 to 310 µM, respectively). Interestingly, the most active diarylpentanoid against L. braziliensis contained p-hydroxyl and m-dimethoxy substituents in one of the aromatic rings (B ring) (74). On the other hand, against L. amazonensis and L. chagasi promastigotes, the most effective derivatives have m-hydroxyl and p-methoxy substituents (72) in the B ring or p-hydroxyl and m-methoxy groups (73) in the B ring [26] (Figure 4). Generally, when comparing the overall results, we can infer that the presence of unsubstituted (78) or monosubstituted (75) B ring is associated with a decrease in antileishmanial activity.
The synthesis and evaluation of the antiproliferative activity of asymmetric diarylpentanoids (79-97: Figure 5) for promastigotes of L. amazonensis and epimastigotes and trypomastigotes of T. cruzi has been reported [21]. It was inferred that these curcuminoids have potent antiproliferative activity against studied parasites, with compounds containing an ethyl substituent at the α-position to the carbonyl group and a p-chloro substituent in the A ring (70-76: IC 50 = 0.5-74.2 µM) being more potent than those with a methyl group at the α-position to the carbonyl group and a p-methoxy substituent in the A ring (79-90: IC 50 = 3.7->100 µM) ( Figure 4). Considering the results reported for the most potent derivatives (91-97), different substitutions in the B ring were accepted, except for the presence of a p-carbonyl group, which was associated with a decrease in activity ( Figure 4). For derivatives 79-90, it was observed that di-or tri-methoxy substitution on the aromatic B ring was more favorable for activity than orthoor para-halogenated substitution on the same ring ( Figure 4). Interestingly, the derivative with a p-methyl group in the A ring and a methyl group at the α-position to the carbonyl group (90) presented a reduction in activity when compared to the other tested compounds ( Figure 4). The binding interactions of potent compounds were predicted through molecular docking computational studies, and strong interactions with the enzyme trypanothione reductase (pdb code 1BZL) were found [21].

Antioxidant Activity
Free radicals and other reactive oxygen species, such as superoxide radical anions, hydroxyl radicals, and hydrogen peroxide, are constantly generated through many biological processes. When the generation of these species are not balanced with natural

Antioxidant Activity
Free radicals and other reactive oxygen species, such as superoxide radical anions, hydroxyl radicals, and hydrogen peroxide, are constantly generated through many biological processes. When the generation of these species are not balanced with natural antioxidants they can lead to oxidative alterations at the cellular and molecular levels, which are associated with various pathologies, such as cancer, cardiovascular diseases, atherosclerosis, hypertension, ischemia/reperfusion injury, diabetes mellitus, neurodegenerative diseases, rheumatoid arthritis, and aging [27][28][29][30]. Thus, the removal of free radicals from biological systems is very important for the sustainability of cellular machinery. A series of diarylpentanoids were prepared, and antioxidant activity was evaluated and compared to curcumin (1) by the scavenging of the diphenylpicrylhydrazyl (DPPH) free radical assay and other assays. Youssef et al. (2004) reported a new series of diarylpentanoids (8: Figure 3; 98-110: Figure 6) for their in vitro DPPH scavenger activity. The free radical scavenging activity was also measured by chemiluminescence using polymorphonuclear leukocytes (PMNs). All compounds showed high or moderate free radical scavenger activity compared to curcumin (1), with compound 103 showing the highest percentage of inhibition. In addition, the results demonstrated that the presence of electron-donating substituents on the metaand para-position of aromatic rings (99, 100, 102-104, 106: K = 7.30-16.32 min −1 × 10 −2 ) is associated with enhanced antioxidant activity when comparing to compounds with only p-hydroxy substituents (98: K = 2.15 min −1 × 10 −2 ; 101: K = 3.06 min −1 × 10 −2 ; 105: K = 14.80 min −1 × 10 −2 ). Additionally, the activity is potentiated by increasing the lipophilicity of the piperidone ring through substitution of methyl (98) with either ethyl (101) or propyl moieties (105) (Figure 7). Comparing the effects of diarylpentanoids with the same aryl rings and different C5 bridges, it seems that the presence of a piperidone (98-106) or cycloheptanone (107 and 108) moiety is much more favorable than the presence of a 3-oxo-penta-1,4-diene moiety (8, 109, and 110) (Figure 7) [31].

Anti-Inflammatory Activity
The persistence of inflammation in the body leads to numerous inflammatory diseases, including pancreatitis, arthritis, inflammatory bowel disease, colitis, gastritis, allergies, autoimmune diseases, cardiovascular problems, atherosclerosis diabetes, arthritis, neurodegenerative diseases, and cancer [38]. The use of anti-inflammatory drugs is associated with several side effects, including gastrointestinal and renal toxicity. Therefore, the development of more efficient and less toxic drugs is progressing worldwide. Anti-inflammatory effects through the diverse mechanisms of diarylpentanoids are well documented. Among these mechanisms, the regulation of short-lived bioactive free radical nitric oxide (NO), as well as pro-inflammatory and inflammatory cytokines and transcription factors, and regulation of enzymatic activity such as that related to cyclooxygenase (COX) and lipoxygenase (LOX) has been highlighted.

Effect of Diarylpentanoids on NO Production
NO, a potent pro-inflammatory mediator, is synthesized by the catalytic reaction of nitric oxide synthase (iNOS) in response of inflammatory stimuli [39]. Abnormal expression of iNOS and excessive generation of NO is expressed in response to various stimuli (lipopolysaccharide (LPS), interferon-gamma (IFN-γ), or tumor necrosis factoralpha (TNF-α)) [40], which enhances the production of diverse inflammatory mediators and leads to alterations in physiological and pathophysiological mechanisms, resulting in inflammatory disorders [41][42][43][44][45].
In the same year, newly synthesized 2-benzoyl-6-benzylidene cyclohexanone analogs (190-213: Figure 6) were investigated for their antioxidant activity in both DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays [37]. The results revealed that diarylpentanoid 199 with a catechol group displayed significant activity in both DPPH (IC 50 = 11.5 µM) and FRAP (0.756 mmol AAE/L) assays, with the DPPH scavenging effect being double that of curcumin (1: IC 50 = 23.6 µM), while it was equipotent to butylated hydroxytoluene in the FRAP scavenging assay (0.803 mmol AAE/L). Compound 206 showed promising results but was worse than curcumin (1) [37]. From these data, we can infer some SAR considerations as follows. Compounds with a single ortho-, meta-, or para-substituent have similar potency independent of the nature of the substituent, namely chloro, methoxy, or hydroxyl groups ( Figure 7). For compounds with di-substituted phenyl rings, the metaand para-positions appear to be the most effective. Moreover, the presence of catechol or m-methoxy and p-hydroxyl groups were important for potency. Nevertheless, the presence of a dimethoxy group in the metaand para-positions leads to loss of activity ( Figure 7).

Anti-Inflammatory Activity
The persistence of inflammation in the body leads to numerous inflammatory diseases, including pancreatitis, arthritis, inflammatory bowel disease, colitis, gastritis, allergies, autoimmune diseases, cardiovascular problems, atherosclerosis diabetes, arthritis, neurodegenerative diseases, and cancer [38]. The use of anti-inflammatory drugs is associated with several side effects, including gastrointestinal and renal toxicity. Therefore, the development of more efficient and less toxic drugs is progressing worldwide. Anti-inflammatory effects through the diverse mechanisms of diarylpentanoids are well documented. Among these mechanisms, the regulation of short-lived bioactive free radical nitric oxide (NO), as well as pro-inflammatory and inflammatory cytokines and transcription factors, and regulation of enzymatic activity such as that related to cyclooxygenase (COX) and lipoxygenase (LOX) has been highlighted.

Effect of Diarylpentanoids on NO Production
NO, a potent pro-inflammatory mediator, is synthesized by the catalytic reaction of nitric oxide synthase (iNOS) in response of inflammatory stimuli [39]. Abnormal expression of iNOS and excessive generation of NO is expressed in response to various stimuli (lipopolysaccharide (LPS), interferon-gamma (IFN-γ), or tumor necrosis factor-alpha (TNF-α) [40], which enhances the production of diverse inflammatory mediators and leads to alterations in physiological and pathophysiological mechanisms, resulting in inflammatory disorders [41][42][43][44][45].
In addition to antioxidant activity, compound 22 (Figure 3), as well as compounds 163 and 164 ( Figure 6), was also tested for its anti-inflammatory activity using the murine monocytic macrophage cell line RAW 264.7. Compound 163, with no additional substituent on both aromatic rings, showed weak activity (32.62% inhibition of NO production), while compounds 22 and 164 showed moderate activity (57.77% and 58.62% inhibition of NO production, respectively) compared to the positive control (L-NAME, 82.84% inhibition of NO production) [33].
In 2014, Leong et al. synthesized a series of 97 asymmetric diarylpentanoids (219-275: Figure 8). The IFN-γ/LPS-stimulated macrophages cells were used to assess anti-inflammatory activity through the NO suppression assay. Among all the series, 12 compounds (226, 238, 240, 244, 255, 256, 262-264, 266, 268, and 275: IC 50 = 13.6-19.8 µM) exhibited greater or similar NO inhibitory activity when compared to curcumin (1: IC 50 = 14.7 ± 0.2 µM), with compounds 268 and 275 being the most potent with IC 50 values of 4.9 ± 0.3 µM and 9.6 ± 0.5 µM, respectively. An SAR study revealed findings in accordance with a previous study, where the presence of a hydroxyl group in both aromatic rings was critical for bioactivity ( Figure 9). Moreover, the presence of a catechol moiety on the B ring appeared to be an important contributing factor since it resulted in a two-to four-fold improvement in bioactivity ( Figure 9). Further structure-activity comparisons showed that the presence of an electron-withdrawing group on the A ring was important for enhancing the NO inhibitory activity. In contrast, replacing the B phenyl ring with a heterocyclic aromatic ring was found to be undesirable ( Figure 9) [46].
In continuation with previous work, Leong et al. [37] investigated 2-benzoyl-6-benzylidene cyclohexanone analogs (190-213: Figure 6) for their in vitro NO inhibitory effects in an LPS/IFN-induced RAW 264.7 macrophages model. The results revealed that compounds 192, 197, 206, and 207 moderately suppress NO production (IC 50 = 22.7-35.3 µM), and compounds 199 and 208 exhibited the highest activity with an IC 50 value of 4.2 ± 0.2 and 15.2 ± 0.8 µM, respectively. Based on these results, it appears that the presence of hydroxyl and methoxy groups in the B ring was imperative for the inhibition of NO production, with the meta-hydroxylated Discovery Studio 3.1, and results indicated that compounds presented adequate drug-like properties [22].

Modulation of Pro-Inflammatory Cytokines and Transcription Factors by Diarylpentanoids
Pro-inflammatory cytokines, such as TNF-α, and interleukins, particularly IL-6, are Another study in 2015 reported a newly synthesized series of diarylpentanoids (276-320: Figure 8) with anti-inflammatory activity by measuring their NO inhibition activity in IFN-γ/LPS-activated RAW 264.7 cells [22]. Among the tested compounds, 295, 316, 319, and 320 showed similar or greater activity (IC 50 = 10.2-16.4 µM) than curcumin (1: IC 50 = 14.7 ± 0.2 µM), with the most significant inhibition of NO production being observed for compound 319 (IC 50 = 10.24 ± 0.62 µM), a 5-methylthiophenyl-bearing analog. Additionally, compounds 278, 296-298, 304, 317, and 318 significantly inhibited NO production, with IC 50 values ranging between 20.0-30.0 µM. Generally, it was observed that the chemical nature of both rings (A and B) plays a significant role in enhancing anti-inflammatory activity in the diarylpentadien-1-one compounds ( Figure 9). Apparently, the presence of a para-hydroxyl group on the B ring is the feature that contributes to NO inhibitory activity. In turn, the presence of metaor para-hydroxyl groups on the A ring is the feature that contributes to NO inhibitory activity, whereas electron-withdrawing groups disfavor the activity (Figure 9). On the other hand, the replacement of the phenyl A ring with a heterocyclic ring, such as thiophenyl or furanyl, resulted in mild inhibition of NO production ( Figure 9). The biological implication of structural features along with 2D and 3D quantitative structure-activity relationship (QSAR) analyses were performed. It was concluded that a hydroxyl group on the para-position of the B ring and an α,β-unsaturated ketone moiety on the linker were important features in the enhanced inhibition of NO production. Additionally, ADMET analysis was performed using Discovery Studio 3.1, and results indicated that compounds presented adequate drug-like properties [22].

Modulation of Pro-Inflammatory Cytokines and Transcription Factors by Diarylpentanoids
Pro-inflammatory cytokines, such as TNF-α, and interleukins, particularly IL-6, are critically involved in inflammation and related disorders, including ulcerative colitis, diabetes, multiple sclerosis, atherosclerosis, and septic shock. The pro-inflammatory effects of TNF are primarily due to its ability to activate the transcription factors (nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1)) responsible for regulating the expression of pro-inflammatory genes involved in controlling cellular proliferation/growth, inflammatory responses, and cell adhesion. Therefore, antiinflammatory agents that inhibit the overexpression of pro-inflammatory cytokines are of great interest for the clinical treatment of many inflammatory diseases.
Later, the same group obtained and tested diarylpentanoids 3, 7, 13, 17, 20, 28, 31, and 32 (Figure 3), as well as 354 (Figure 10), for their anti-inflammatory activity against LPS-induced TNF-α and IL-6 secretions using J774.1 mice macrophages [49]. Compounds meta-methoxylated (20) and para-halogenated (28) in the phenyl rings exhibited the maximum anti-inflammatory activity at a concentration of 10 µM with 40% TNF-α inhibition and 50% IL-6 inhibition, respectively. The remaining compounds showed moderate activity at the same concentration. The results showed that the presence of meta-methoxy groups played an important role in bioactivity (20, 32, and 354) compared to unsubstituted compounds (7, 17, and 31), and compounds with a cyclohexanone moiety exhibited stronger inflammatory inhibition than compounds with a cyclopentanone moiety or an acyclic C5 bridge (Figure 11), which conforms with a previous study [48]. Single-crystal XRD was then performed, and it was concluded that the presence of a cyclohexanone moiety may play an important role in bioactivity [49].
In Compound 368, possessing orthoand meta-dimethyl-substituted phenyl rings, showed the strongest inhibitory effect on LPS-induced TNF-α and IL-6 release (34.3% and 15.9%, respectively) ( Figure 11). From the active analogs above, four compounds, 21, 23, 355, and 374, were selected for further evaluation of their inhibitory effects against LPS-induced TNF-α and IL-6 release. Compounds 21, 23, and 374 demonstrated comparable inhibitory effects to curcumin (1). The results of this study were in accordance with the data obtained previously by   [49].
Synthesis and anti-inflammatory evaluation of a series of diarylpentanoids with different C5 bridges (acyclic bridge: 329, 383-388, Figure 10; cyclopentanone moiety: 389-395, Figure 10; and cyclohexanone moiety: 396-404, Figure 10 , and compounds 329, 384, 385,  389, 388, 393, 400, and 404 showed inhibitory effects on LPS-induced IL-6 production, with 388 and 404 being more potent than curcumin (1) in the production of both cytokines in a dose-dependent manner. From these results, some SAR considerations may be undertaken ( Figure 11). Contradicting the study conducted by , it was suggested that compounds with a C5 acyclic bridge were found to be more effective than compounds with cyclopentanone or cyclohexanone moieties. Additionally, the electron-donating ability of the para-substituent on the aromatic rings might be responsible for enhancing the antiinflammatory abilities of diarylpentanoids 329, 384, 385, 389, 390, 392, 397, 398, and 400, whereas compounds without substituents at the para-position (386, 387, 391, 393, 394, 399,  401, and 402) and an electron-withdrawing moiety (384, 329, 396) may reduce bioactivity. Additionally, aminated analogs (404) may be considered as promising compounds for developing anti-inflammatory candidates ( Figure 11) [50].  Another novel series of allylated or prenylated diarylpentanoids (405-437: Figure 10) were designed, synthesized, and investigated against TNF-α and IL-6 expression induced by LPS using mouse RAW 264.7 macrophages. The majority of the tested compounds inhibited the LPS-induced expression of TNF-α and IL-6, particularly IL-6 expression, at a dosage of 10 µM. Their inhibitory abilities were comparable to or more pronounced than curcumin (1) at the same concentration. It was seen that compounds 407, 433, 434, and 437 exhibited stronger inhibition of both TNF-α and IL-6 than curcumin (1), with 433 being the most potent on LPS-induced TNF-α and IL-6 production with its inhibitory rates reaching 67.5% and 91.1%, respectively. Compound 433 also exhibited significant protection against LPS-induced death in septic mice. SAR studies suggested that the electronic properties of the substituents on the aromatic rings seem to have no influence on bioactivity, and the dior tri-substituted compounds are much more active than mono-substituted compounds ( Figure 11) [51].

Regulation of COX and LOX Pathways by Diarylpentanoids
Regulation of the activity of cyclooxygenase (COX) and 5-lipoxygenase (5-LOX), involved in the biosynthesis of prostaglandins (PGs), leukotrienes (LTs), and thromboxanes (Tx), plays an important role in the process of inflammation and associated diseases. Thus, inhibition of the biosynthesis of these inflammatory mediators has been proposed as a potential therapy for different inflammatory disorders.
Diarylpentanoids with phenyl (568: Figure 12), naphthyl (569: Figure 12, and heterocyclic rings (570-577: Figure 12) were examined for their anti-inflammatory activity in both in vitro and in vivo assays. Among these compounds, 569 was the most potent LOX inhibitor (IC50 = 37 µM), followed by 570-572 (IC50 = 280-410 µM). From these results, some SAR considerations may be undertaken ( Figure 13). The presence of heterocyclic rings is associated with high anti-inflammatory activity (570-572), except for imidazole rings (574). Contrarily, diarylpentanoids with phenyl rings (568 and 573) showed low activity. Nevertheless, the presence of methyl groups in compounds with heterocyclic rings (575 and 576) appears to be unfavorable for activity. Lastly, the acryloyl group in piperidone (577) is tolerated. Docking studies were performed with compound 569 on LOX, and it was observed that allosteric interactions may govern LOX-inhibitor binding [58].
Symmetric cyclic and acyclic diarylpentanoids (217, 328, 351, 362, 578-591: Figure 12) were evaluated for their effects on the enzymes of the arachidonic acid (AA) cascade,  Figure 10) and evaluated their effect on the inhibition of LPS-induced TNF-α and IL-6 release in a model of mouse RAW 264.7 macrophages. The reported data revealed that all tested compounds presented stronger anti-inflammatory activity than curcumin (1). However, compounds 438, 440, 441, 447, and 449 manifested highly significant and dose-dependent anti-inflammatory activity. Additionally, the inhibition of cytokine production caused by compounds 438 and 440 was associated with the inhibition of ERK phosphorylation and the NF-κB pathway. Furthermore, compound 440 exhibited significant protection against LPSinduced septic death in vivo. The overall results suggest that the existence of a methoxy group in the B ring, and the presence of heterocyclic rings (449 and 450), is associated with increased anti-inflammatory activity ( Figure 11). However, the inhibitory effect on IL-6 production is enhanced when an electron-donating hydroxyl group is positioned in the A phenyl ring (438-450), while an electron-withdrawing chlorine in the A phenyl ring has little effect (451-465) ( Figure 11) [52].
In 2014, Zhang et al. also reported asymmetric (464-492: Figure 10) and symmetric (493-497: Figure 10) diarylpentanoids as potent anti-inflammatory agents. Some of the synthetic analogs exhibited similar or more potent inhibitory ability than curcumin (1), with symmetric compounds 493-497 as well as an asymmetric compound 493 with a tetrahydro-4H-thiopyran-4-one moiety being the most effective as inhibitors of LPS-induced TNF-α and IL-6 secretion (with a range of 59.5%-83.4%) in mouse macrophages. A compound with a thiopyranone moiety (497) showed the most potent activity, with an inhibition of 98.7%. Mechanistically, compound 493 significantly inhibited the LPS-induced phosphorylation of ERK. Furthermore, diarylpentanoids 493 and 496 displayed a significant protective effect on LPS-induced septic death in mouse models, with 40% and 50% survival rates, respectively. The results showed that molecular symmetry and electronegativity might play a crucial role in the anti-inflammatory activity of these diarylpentanoids ( Figure 11) [53].
A series of symmetric 1,5-diphenyl-3-oxo-penta-1,4-dienes (498-519: Figure 10) were synthesized and evaluated for their anti-inflammatory activity using in vitro assays and in vivo models [54]. Most of the tested compounds significantly reduced the production of TNF-α and IL-6 in LPS-stimulated macrophages when compared to curcumin (1). Compounds 503, 506, 512, and 519 exhibited a high degree of inhibitory effects on TNF-α expression, and compounds 501, 503, 507, 508, 510, 517, and 519 showed remarkable downregulation of IL-6 expression stimulated by LPS, with 503, having an ortho-nitro-substituted phenyl ring, possessing the most potent inhibition of LPS-induced IL-6 and TNF-α production (an inhibition rate of 54.53% and 91.20%, respectively). The mechanism of action for compound 503 might be associated with inhibition against LPS-induced NF-κB and ERK pathway activation. Further, treatment with 503 effectively prolonged survival in a mouse model of LPS-induced sepsis. SAR analysis of synthetic analogs revealed that the presence of an electron-withdrawing substituent on the B ring (502, 503, and 512) seems to contribute to the highest inhibition of TNF-α production ( Figure 11). Further investigation into the possible mechanism revealed that the anti-inflammatory activity of the analog 512 might be correlated with its inhibition on NF-κB and ERK pathway activation [54].
A range of 3,5-bis(arylmethylene)piperidin-4-ones (520-532: Figure 10) and related N-cyclopropyl analogs (533-537: Figure 10) were prepared and evaluated for their ability to interfere with LPS-induced TNF-α and IL-6 expression using both in vitro and in vivo models. Most of these compounds exhibited significant inhibition of IL-6 expression but almost no activity toward TNF-α levels, except compound 526 (% inhibition = 39.4%). It was observed that the incorporation of various alkyl groups in the piperidone motif (522-526) improved IL-6 expression inhibition compared to the unsubstituted compound (521) (Figure 11). Moreover, if the substituents in the nitrogen atom were substituted by benzyloxy groups (528-532), more potent IL-6 inhibition was detected ( Figure 11). Compound 543 significantly inhibited both cytokines at a concentration of 10 µM. This compound (533) was further evaluated for in vivo inhibitory activity and results revealed that it caused a significant and dose-dependent inhibition of cytokine production [55].
In a model of LPS-induced IL-6 and TNF-α release, the inhibitory effects of a series of resveratrol and diarylpentanoid hybrids (538-564: Figure 10) was evaluated in vitro (using the MTT assay on the normal human hepatic cell line HL-7702) and in vivo [56]. Overall, the curcuminoids with an acyclic C5 bridge were more effective in preventing the expression of IL-6 and TNF-α compared to the control compound, resveratrol, or other curcuminoids with a cyclic C5 bridge (539-543). Particularly, compounds 459, 473, 547, 550, and 558 showed significant anti-inflammatory activity in a dose-dependent manner with low toxicity in vitro. Diarylpentanoid 547 decreased LPS-induced TNF-α, IL-6, IL12, and IL-33 mRNA expression. Additionally, 547 significantly protected against LPS-induced acute lung injury in an in vivo mouse model. SAR analysis indicates the influence of different C5 bridges and the relation of the position and number of substituent groups on the aromatic rings with anti-inflammatory activity ( Figure 11). Firstly, it was observed that the penta-1,4-dien-3-one linker was more effective in preventing the expression of IL-6 or TNF-α ( Figure 11). As for the mono-substituted compounds, the presence of electron-withdrawing substituents (538 and 544) was more favorable for activity than the presence of electron-donating groups (553), with meta-position (544) being better than ortho-position (538) in terms of the phenyl ring. On the other hand, the presence of a hydrophilic group at the para-position of the phenyl ring (554 and 559) was significantly associated with higher inhibitory activity than the presence of a hydrophobic group (546, 549, and 552). Additionally, for di-or tri-substituted compounds in the aromatic rings (547 and 555-562), the introduction of an electron-withdrawing group at the orthoposition and an electron-donating group at the meta-position resulted in enhanced antiinflammatory activity, except for compound 561. The presence of a heterocyclic ring (565) also contributed to the anti-inflammatory effect [56].

Regulation of COX and LOX Pathways by Diarylpentanoids
Regulation of the activity of cyclooxygenase (COX) and 5-lipoxygenase (5-LOX), involved in the biosynthesis of prostaglandins (PGs), leukotrienes (LTs), and thromboxanes (Tx), plays an important role in the process of inflammation and associated diseases. Thus, inhibition of the biosynthesis of these inflammatory mediators has been proposed as a potential therapy for different inflammatory disorders.
Symmetric diarylpentanoid 23 (Figure 3), (2E,5E)-2,5bis(4-hydroxy-3-methoxybenzylidene), was synthesized and its effect on the blood glucose level and lipid profile of normoglycemic and streptozotocin (STZ)-induced diabetic rats was investigated [61]. In an oral glucose and sucrose tolerance test, treatment with 23 (25 mg/kg) and the standard drug glidenclamide (10 mg/kg) significantly improved glucose tolerance and significantly reduced AUC glucose compared to the NC group. In turn, treatment with 23 (25 mg/kg) and the α-glucosidase inhibitor acarbose (7 mg/kg) exhibited a significant reduction in SG level over the period of 120 min compared to the NC group. Furthermore, treatment with 23 significantly improved sucrose tolerance in normal rats, suggesting that 23 inhibited α-glucosidase. Lipid profile studies also support the antidiabetic efficacy of 23. These results suggested that meta-methoxylated and para-hydroxylated positions were important for the potential antidiabetic effect of diarylpentanoids. These data suggested that the potency of the compound is comparable to the standard drug glidenclamide [61].

Anti-hyperuricemic Activity
A set of asymmetric diarylpentanoids with cyclohexanone and cyclopentanone moieties (640-661: Figure 16) was synthesized and tested for its effectiveness as an antihyperuricemic agent by using hyperuricemic mice models induced with oxonate treatment [65]. Generally, compounds exhibited remarkable anti-hyperuricemia activity,  Figure 14) and investigated its effects on hyperglycemia-induced inflammation and fibrosis in NRK-52E and H9C2 cells through a streptozotocin-induced diabetic mouse model. It was observed that compound 514 exerted significant inhibitory effects on hyperglycemia-induced inflammation and fibrosis in NRK-52E and H9C2 cells, with these effects being associated with the inhibition of the P38 and AKT signaling pathway, respectively. Diabetes was induced in mice by a single intraperitoneal injection of freshly prepared STZ (100 mg/kg in citrate buffer, pH = 4.5). The group treated with 614 experienced a slight reduction in blood glucose compared to the STZ-treated group. The 614-treated mice presented indicators of decreased heart function but the indicators of kidney function did not change [63]. These results demonstrated that symmetry is not a requirement for the antidiabetic potential of diarylpentanoids ( Figure 15).

Neuroprotector Activity
Among neurodegenerative disorders, Alzheimer's disease (AD) is one of the most common, leading to progressive and irreversible neurodegeneration characterized by permanent memory loss, cognitive impairment, disorientation, confusion, and language deficits [66]. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are important enzymes that catalyze the degradation of acetylcholine, an important neurotransmitter involved in memory and cognition. Targeting AChE and BChE may be one of the most promising approaches for treating AD [67,68]. Interestingly, it was reported that BChE activity is dramatically increased when AChE is inhibited, which implies that dual inhibitors are a better choice for treating some diseases.

Neuroprotector Activity
Among neurodegenerative disorders, Alzheimer's disease (AD) is one of the most common, leading to progressive and irreversible neurodegeneration characterized by permanent memory loss, cognitive impairment, disorientation, confusion, and language deficits [66]. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are important enzymes that catalyze the degradation of acetylcholine, an important neurotransmitter involved in memory and cognition. Targeting AChE and BChE may be one of the most promising approaches for treating AD [67,68]. Interestingly, it was reported that BChE activity is dramatically increased when AChE is inhibited, which implies that dual inhibitors are a better choice for treating some diseases.

Interference with Diverse Biochemical Targets
In addition, a total of 83 diarylpentanoids have also been described for their ability to interfere with several proteins, namely enzymes and channels.

Interference with Diverse Biochemical Targets
In addition, a total of 83 diarylpentanoids have also been described for their ability to interfere with several proteins, namely enzymes and channels.
1 Figure 18. Diarylpentanoids described for their ability to interfere with several proteins.  Figure 18. Diarylpentanoids described for their ability to interfere with several proteins.  Alkaline and acid phosphatase are enzymes of hydrolase classes (EC 3.1.3.1 and EC 3.1.3.2, respectively) that are found in all living tissues and are involved with the removal of the phosphate from protein and other molecules. These enzymes are used as diagnostic tools in various pathological conditions [71,72]. In 2014, Singh et al. tested symmetric diarylpentanoids with a cyclic C5 bridge (28: Figure 3; 165-167: Figure 6; 351: Figure 10; and 699-701: Figure 18) for their ability to interact with acid and alkaline phosphatases [73]. Although compounds have presented inhibitory activity (% of inhibition~40-75%) with alkaline phosphatases, an activating effect was detected for acid phosphatases (% activatioñ 25-60%). Additionally, molecular modeling and docking of these compounds into alkaline phosphatase using iGemdock was performed. All compounds showed greater affinity to alkaline phosphatase than the control levamisole. All compounds inhibited enzyme activity in a competitive manner, except compound 700, whose inhibitory effect was shown to be non-competitive [73].
Carbonic anhydrase II (CA-II) is one of sixteen forms of human α-carbonic anhydrases, an enzyme of the lyase class (E.C.4.2.1.1). The isozyme CA-II is known to be one of the most efficient in CO 2 hydration. Several diseases are associated with CA deregulation, such as glaucoma, hypertension, edema, epilepsy, and cancer; thus, the development of CA inhibitors has been a strategy for the treatment of CA-related disorders [74]. Aditama et al. (2017) described in vitro and in silico studies of diarylpentanoids (98: Figure 6; 702-738: Figure 18) as inhibitors of CA-II. The authors reported that all compounds exhibited low or moderate inhibition, except for 702 that showed high inhibitory activity (IC 50 = 7.92 µM). The presence of hydroxyl groups at the ortho-position (702) of both aromatic rings is associated with high inhibition against CA-II compared to the presence of hydroxyl groups at the meta-(705, 716, and 729) or para-positions (98 and 732), as well as compared to the presence of methoxy groups ( Figure 19). Additionally, the substitutions on the 4-piperidone core seemed to interfere with inhibitory activity, since compounds 713 and 726 with the Nmethyl-4-piperidone and N-propyl-4-piperidone moiety, respectively, exhibited contrasting results to 702, which possesses an unsubstituted 4-piperidone core ( Figure 19). Lastly, molecular docking analysis revealed that compound 702 formed hydrogen interactions with Thr199, Thr200, and Gln92 at the active site of CA-II [75].
The transient receptor potential (TRP) channels of ankyrin type-1 (TRPA1) and vanilloid type-1 (TRPV1), which belong respectively to the TRPA and TRPV subfamilies of the large TRP cation channel family, play numerous roles in many physiological and pathophysiological processes and are involved in the perception of nociceptive and inflammatory pain via sensory nerve activation. In 2017, the ability to interact with channels TRPA1, TRPM8, and TRPV1 for a series of symmetric diarylpentanoids (3, 9, 13, 23, and 28: Figure 3; 163, 165, and 167: Figure 6; 327, 334, and 346: Figure 10; and 739-745, Figure 18

Conclusions
Diarylpentanoids are important scaffolds in medicinal chemistry which are known for their great variety of biological activities. In this review, a total of 745 diarylpentanoids with a wide range of biological activities, beyond just antitumor activity, have been described. The most commonly employed method for the synthesis of symmetric diarylpentanoids is Claisen-Schmidt condensation. However, other methods have been reported to obtain asymmetric diarylpentanoids.
Among biological activities, anti-inflammatory activity has been the most studied, with the modulation of pro-inflammatory cytokines and transcription factors being the most reported ( Figure 20). Considering the diarylpentanoids with anti-infective activity, it was observed that only symmetric diarylpentanoids exhibited antibacterial activity, and only asymmetric acyclic compounds were found to be antiparasitic agents. Anti-hyperuricemic and neuroprotector activities were the least reported, and only asymmetric diarylpentanoids could be found in the literature. On the other hand, for antioxidant, antiinflammatory, and antidiabetic activities, both symmetric and asymmetric diarylpentanoids have been reported.
described. The most commonly employed method for the synthesis of symme diarylpentanoids is Claisen-Schmidt condensation. However, other methods have b reported to obtain asymmetric diarylpentanoids.
Among biological activities, anti-inflammatory activity has been the most stud with the modulation of pro-inflammatory cytokines and transcription factors being most reported (Figure 20). Considering the diarylpentanoids with anti-infective activ it was observed that only symmetric diarylpentanoids exhibited antibacterial activity, only asymmetric acyclic compounds were found to be antiparasitic agents. A hyperuricemic and neuroprotector activities were the least reported, and only asymme diarylpentanoids could be found in the literature. On the other hand, for antioxidant, a inflammatory, and antidiabetic activities, both symmetric and asymme diarylpentanoids have been reported. This review shows that the diarylpentanoid scaffold still gathers a lot of attent not only from a structural variety point of view, but also because of pharmacological biological properties. Information about the most relevant molecular features of biological activities provided in this manuscript can be useful for designing new bioac diarylpentanoids with improved therapeutic activity against diverse diseases.
Supplementary Materials: The following supporting information can be downloaded www.mdpi.com/xxx/s1, Table S1: Diarylpentanoids with biological effects beyond antitu activity; References citation of [77]. Funding: This research was supported by national funds through the Foundation for Science Technology (FCT) within the scope of UIDB/04423/2020, UIDP/04423/2020 (CIIMAR, Grou Natural Products and Medicinal Chemistry), and UID/QUI/50006/2020 (LAQV/REQUIMTE well as under the PTDC/CTA-AMB/0853/2021 project co-financed by COMPETE 2020, Portugal and with further support provided by the European Union through the ERDF and by FCT thro national funds supported by NORTE2020 (through ERDF CHIRALSINTESE_APSFCT_IINFACTS_2021). This work was also funded by the struct program R&D&I ATLANTIDA-Platform for the monitoring of the North Atlantic Ocean and t This review shows that the diarylpentanoid scaffold still gathers a lot of attention, not only from a structural variety point of view, but also because of pharmacological and biological properties. Information about the most relevant molecular features of the biological activities provided in this manuscript can be useful for designing new bioactive diarylpentanoids with improved therapeutic activity against diverse diseases.
Supplementary Materials: The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/molecules27196340/s1, Table S1: Diarylpentanoids with biological effects beyond antitumor activity; References citation of [77]. Funding: This research was supported by national funds through the Foundation for Science and Technology (FCT) within the scope of UIDB/04423/2020, UIDP/04423/2020 (CIIMAR, Group pf Natural Products and Medicinal Chemistry), and UID/QUI/50006/2020 (LAQV/REQUIMTE), as well as under the PTDC/CTA-AMB/0853/2021 project co-financed by COMPETE 2020, Portugal 2020 and with further support provided by the European Union through the ERDF and by FCT through national funds supported by NORTE2020 (through ERDF and CHIRALSINTESE_APSFCT_IINFACTS_2021). This work was also funded by the structured program R&D&I ATLANTIDA-Platform for the monitoring of the North Atlantic Ocean and tools for the sustainable exploitation of marine resources (reference NORTE-01-0145-FEDER-000040), as supported by the North Portugal Regional Operational Programme (NORTE2020) through the European Regional Development Fund (ERDF). Joana Moreira acknowledges her PhD grant (SFRH/BD/135852/2018).