Growth Stimulation, Phosphate Resolution, and Resistance to Fungal Pathogens of Some Endogenous Fungal Strains in the Rhizospheres of Medicinal Plants in Vietnam

Endophytic fungi are recognized for their many potential applications in agriculture, such as supporting cropland expansion and increasing the yield and resistance of plants by creating antibiotics that inhibit the growth of pathogenic microorganisms. In addition, they can produce enzymes that break down hard-to-solubilize substances within soil, dissolve phosphates, fix nitrogen, reduce metals, and produce hormones that promote plant growth (auxin, cytokinin, and gibberellins) to keep crops healthy. In this report, three strains of endophytic fungi, namely, N1, N2, and N3, were isolated from the roots of Stevia rebaudiana (Bert.) Hemsl., Polyscias fruticosa, and Angelica dahurica in some localities in Vietnam. Through a screening process, it was found that they can produce high levels of indole acetic acid (IAA), resolve phosphates, and resist disease, and they were selected to as an alternative to chemical fertilizers to make probiotics in order to increase medicinal plant yields. The results show that the three strains of fungi have the ability to degrade phosphate to 341.90, 1498.46, and 390.79 ppm; the content of IAA produced in the culture medium reached 49.00, 52.35, and 33.34 ppm. Based on some morphological characteristics and an internal transcribed spacer gene sequence analysis of the fungal strains, N1, N2, and N3 were named Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3, respectively, which have the ability to inhibit the growth of pathogenic fungal strains, such as fungus C. gloeosporioides (CD1), fungus F. oxysporum, fungus L. theobromae N13, and N. dimidiatum. They grow significantly over a period of 5 to 6 days.


Introduction
The demand for medicinal herbs and medicinal products is increasing as the world attempts to reshape research methods in order to find new drugs rather than focusing solely on the synthesis of chemicals in the laboratory, a procedure with many difficulties resulting in toxic chemicals. Research and experiments on nature conducted to identify new bioactive substances with more favorable, less toxic properties are receiving a lot of attention.
Endophytic bacteria colonize plants and live inside them for part of or throughout their life without causing any harm or disease to their hosts. Endophytes promote plant growth and fitness through the production of phytohormones or biofertilizers, or by alleviating Endophytic fungi also manifest themselves as plant stimulants to produce self-protective active substances commonly known as phytoalexins [13].
Phosphorus is the second most important macronutrient after nitrogen for plants. It is an indispensable part of plants in general and of medicinal plants in particular. However, the main source of phosphorus is found in soil in the form of complexes with iron, calcium, and aluminum, which cannot be used by plants [14]. Deficiency phosphorus can slow plant growth and reduce leaf biomass [15,16]. Using chemical fertilizers to supply plants with their nutritional needs is extremely popular in agriculture, but it is not environmentally friendly due to non-biodegradable inorganic compounds. Using fungal strains is seen as a useful alternative solution due to their ability to decompose phosphates by creating various organic acids, such as tartaric acid, succinic acid, oxalic acid, malic acid, 2-ketogluconic acid, glyoxylic acid, gluconic acid, fumaric acid, citric acid, and alpha-ketobutyric acid, and the enzyme phosphatase. They are phosphate soluble, and plants can use the dissolved inorganic phosphate form.
IAA is the most common auxin-class phytohormone, and it plays an important role in the growth of plants, as well as contributing to xylem and phloem structures. IAA is a product of L-tryptophan metabolism, it is produced by some microorganisms around the roots, supporting plant growth. It promotes root elongation by increasing the number of lateral roots that take up nutrients [17].
Among 27 fungal strains isolated from the root and rhizospheres of medicinal plants (data not shown here), three strains, namely, N1, N2, and N3, which have the highest ability to produce IAA growth stimulants and degrade insoluble phosphate, were selected. Their biggest advantages are their ability to produce IAA, produce its growth stimulants, and decompose insoluble phosphates.
The selected fungal strains are resistant to pathogenic fungi, degrade phosphate, and produce growth stimulant IAA from the roots of very promising medicinal plants for application in the production of plant support preparations [14,18]. Furthermore, research into the mycorrhizal zone on medicinal plants is essential in identifying diversity and finding potential strains for the production of products with the aim of improving the productivity and quality of medicinal plants, preserving the precious medicinal resources in Vietnam.

Identification of Most Potent Endophytic Fungal Strains
Endophytic microorganisms contribute to the growth of plants and the recovery of plant health by using different strategies, including controlling phytopathogens and secreting phytohormones, such as IAA, cytokinins, and gibberellic acids. Additionally, endophytes can reinforce plant growth by nitrogen fixation, phosphate solubilization, nutrient cycling, and novel and bioactive metabolite secretion. Secondary metabolites secreted by endophytic microbes have various biotechnological applications [19][20][21].
We isolated the rhizomic symbiotic fungal systems of three plant samples and found 27 specific fungal strains: 07 strains of Stevia rebaudiana (rhizospheres: 4 strains; soil: 3 strains), 11 strains of Polyscias fruticosa (roots: 6 strains; soil: 5 strains); and Angelica dahurica: 9 strains (roots: 6 strains; soil: 03 strains). Felde et al. [22] demonstrated that the addition of endophytic fungi Trichoderma atroviride and F. oxysporum improved and increased banana yield, and it reduced the number of parasitic nematodes on banana Radopholus similis. The endophytic fungi Sebacina vermifera, Piriformospora indica, Colletotrichum, and Penicillium have better plant-growth-promoting effects under adverse conditions due to their ability to synthesize enzymes and plant growth promoters, such as auxins, cytokinins, and gibberellins [23]. The fungal strain Talaromyces flavus has also been selected as a promoter to promote the growth of cotton and potato plants due to its high biosynthetic capacity of IAA [24]. Thus, we selected the fungal strains N1, N2, and N3, which have the highest ability to produce IAA growth stimulants and to degrade insoluble phosphate. In general, the fungal strains isolated from the roots were more abundant than those isolated from the soil. The fungal strains, after isolation and cleaning, were grown on potato agar to observe the morphological characteristics and the color of the mycelium, as well as the pigment produced in the medium, and the fungi were grouped according to the color of the mycelium; the results are shown in Table 1. They were then fermented in potato liquid medium. The results are shown in Table 2.  The results show that strain N1 was closest to the species Penicillium genus (100% similarity), strain N2 had 100% similarity with the fungal strain of the Talaromyces genus, and strain N3 had a high similarity with the fungal strain of Trichoderma (100% similarity). The endophytic fungi strains were Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3, respectively. Some fungal species, including P. bilaji, Penicillium spp. [25], P. oxalicum [26,27], Aspergillus niger, Penicillium notatum [28,29], Aspergillus awamori [30], Penicillium bilaii [31], Trichosporon beigelii, Rhodotrula aurantiaca, Cryptococcus luteolus, Zygoascus hellenicus, P. purpurogenum var. rubrisclerotium, Neosartorya fisheri and Candida montana [32], Talaromyces aurantiacus, Aspergillus neoniger [33], and Trichoderma spp. [34], have been reported to solubilize phosphorus. Babu et al. (2015) [35] also found that Penicillium menonorum can enhance plant growth and induce IAA.
Patil et al. conducted a study to evaluate the effects of P-solubilizing fungi and phosphorus levels on the growth, yield, and nutrient content of maize [25]. The field experiment was conducted to test the effects of the P-solubilizing fungi Penicillium bilaji and Penicillium spp. On the availability of applied P-fertilizer in calcareous soil at MARS, University of Agricultural Sciences, Dharwad, during the rabi/summer season of 2009-2010. Seed inoculation with phosphorus-solubilizing fungi, in addition to P 2 O 5 levels, significantly influenced plant height, the number of leaves per plant, dry matter production, cob length, grain weight per cob, 1000 grain weight, grain yield, and tissue nutrient content (N, P, K, Zn, and Fe) at the tasseling of leaves and the harvest of the whole plant, as well as P uptake at harvest. Stover yield was not significantly influenced by the various treatments. Higher growth and a higher yield of maize were achieved when using P-solubilizing fungi treatment and 100% RD P 2 O 5 application compared to 0 and 50% RD P 2 O 5 . It was concluded that single and dual inoculations along with P-fertilizer had 20-23% higher maize yields than the control.
Phosphate-solubilizing fungi (PSF) have huge potential to enhance the release of phosphorus from fertilizer. Two PSF were isolated and identified as Penicillium oxalicum and Aspergillus niger in a study conducted by Li et al. [26]. To enhance the fertilizer value of rock phosphate in alkaline soils, the phosphate (P)-solubilizing fungus Penicillium oxalicum was isolated from the rhizosphere soil of rock phosphate mine landfills, and it was tested for its efficacy to solubilize rock phosphate (RP) and to promote the growth of wheat and maize plants grown in soil amended with RP. The results showed that P. oxalicum effectively solubilized RP in Pikovskaya (PVK) medium and released a high amount of phosphorus [27]. In a previous study, the Azotobacter (SR-4) strain was found to be an efficient nitrogen fixer, as 35.08 mg of nitrogen per gram of carbon was produced after 72 h of fermentation. Similarly, the A. niger strain was found to excrete extracellular phosphatesolubilizing enzymes, such as phytase (133UI in 48 h of fermentation) and phosphatase (170UI in 48 h of fermentation), which can solubilize rock phosphate and make it available to plants. Furthermore, plants co-inoculated with both N-fixing Azotobacter and phosphorussolubilizing A. niger have been found to have enhanced performance compared to those treated with each biofertilizer alone [28]. PSF function in the soil phosphorus cycle by increasing the bioavailability of soil phosphorous for plants. In a previous study, Aspergillus niger and Penicillium notatum were tested for their efficacy to solubilize tricalcium phosphate (TCP) in vitro, as well as for their ability in vivo to promote the growth of groundnut (Arachis hypogaea) plants grown in soil amended with TCP. The results showed a high solubilizing index in agar plates. Moreover, they effectively solubilized TCP in PVK liquid medium and released considerable amounts of P into the medium. A pot experiment showed that the dual inoculation of phosphate-solubilizing fungi (A. niger and P. notatum) significantly increased the dry matter and yield of groundnut plants as compared to control soil [29].
In a previous study, a phosphate-solubilizing fungus, Aspergillus awamori S29, was isolated from the rhizosphere of mungbean. The phosphate-solubilizing activity of A. awamori S29 in liquid was 1110 mg/L for TCP. The organism was able to solubilize various inorganic forms of phosphate at a wide range of temperatures. Among the various insoluble phosphate sources tested, di-calcium phosphate was solubilized the most, followed by TCP. A. awamori S29 had a significant effect (p < 0.05) on mungbean growth, total P, and plant biomass under pot conditions, although no obvious differences in available P in the soil and the number of leaves were found compared to the control [30]. In view of this, field experiments were conducted to evaluate the effect of seed inoculation with PSF (Penicillium bilaii) at different rates of fertilizer P on P content in leaves and the grain yield of irrigated wheat in India. The soil was low in Olsen P at the Bathinda site and had a medium level at the Ludhiana site. In the no-P treatment, PSF significantly increased grain yield by 12.6% over the non-inoculated control. The effect of PSF on grain yield was generally more pronounced in soil with a low Olsen-P level compared to soil with a medium Olsen-P level. The inoculation of PSF along with 50% P fertilizer increased wheat yield equivalent to that of 100% P with no PSF. Spike density was significantly higher in PSF + 50% P than all other treatments. There is a need to study the long-term effect of Penicillium bilaii on P-fertilizer saving in wheat on soils varying in P availability, pH, and P fixation capacity for different wheat-based cropping systems [31]. In the study conducted by Zhang et al. (2018), two PSF, TalA-JX04 and AspN-JX16, were isolated from the rhizosphere soil of moso bamboo (Phyllostachys edulis), which is widely distributed in P-deficient areas in China, and they were identified as Talaromyces aurantiacus and Aspergillus neoniger. The two PSF were cultured in potato dextrose liquid medium with six types of initial pH values ranging from 6.5 to 1.5 to assess acid resistance. Both PSF were incubated in PVK liquid media, with different pH values, containing five recalcitrant P sources, namely, Ca 3 (PO 4 ) 2 , FePO 4 , CaHPO 4 , AlPO 4, , and C 6 H 6 Ca 6 O 24 P 6 , to estimate their P-solubilizing capacity. The P-solubilizing capacity of TalA-JX04 was the highest in the medium containing CaHPO 4 , followed by Ca 3 (PO 4 ) 2 , FePO 4 , C 6 H 6 Ca 6 O 24 P 6 , and AlPO 4 , in the five types of initial pH treatments, while the recalcitrant P-solubilizing capacity of AspN-JX16 varied with initial pH. Meanwhile, the P-solubilizing capacity of AspN-JX16 was much higher than that of TalA-JX04. The pH of the fermentation broth was negatively correlated with P-solubilizing capacity (p < 0.01), suggesting that the fungi promote the dissolution of P sources by secreting organic acids. Their results showed that TalA-JX04 and AspN-JX16 can survive in acidic environments and that both fungi had a considerable ability to release soluble P by decomposing recalcitrant P-bearing compounds [33]. Acidic soils rapidly retain applied phosphorus fertilizers and, consequently, present a low availability of this nutrient to plants. The use of phosphate-solubilizing microorganisms to aid plant phosphorus absorption is a promising sustainable strategy for the management of P deficiencies in agricultural soils. In a previous study, 19.5% of isolated Trichoderma strains were able to solubilize phosphate. In addition, those strains produced different organic acids during the solubilization process. Trichoderma spp. strains showed positive responses in the promotion of soybean growth-from 2.1% to 41.1%-as well as in the efficiency of P uptake, up to 141% [34].
Thus, it can be seen that the fungal strains isolated from the roots of medicinal plants in Vietnam belong to the same species as the fungal strains previously isolated and proven to be able to degrade insoluble phosphate and produce IAA across the world. A phylogenetic tree was built using MEGA X software (Figure 1).

Growth Dynamics of Fungal Strains
Fungal strains often have different optimal biomass growth times. Determining the optimal growth time has a great influence on the practical application of biomass for inoculant production. Therefore, three strains, Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3, were studied for their growth dynamics.
Based on the results, it was found that all three strains of fungi produced the highest biomasses at 144 h after the culture on PDB medium and shaking at 200 rpm at 30 • C, with the biomasses reaching 175.4 g/L for Penicillium simplicissimum CN7, 108.4 g/L for Talaromyces flavus BC1, and 91.2 g/L for Trichoderma konilangbra DL3. This result provides a significant contribution by demonstrating how to obtain strains in production and use them in practice (Figure 2). In this study, after 168 and 192 h, the fermentation pH tended to increase gradually, and the strains Penicillium simplicissimum CN7 and Trichoderma konilangbra DL3 formed pellets with small, smooth, tightly coiled spheres, which obstructed the respiratory process and affected the biological production growth of the fungal strains, while the filamentous Talaromyces flavus BC1 strain was less affected. In a previous study conducted by Zhang et al., 2018, the fungus strain Talaromyces JX04, which was found to degrade insoluble phosphates isolated from the soil around bamboo roots in Jiangxi, China, produced the highest biomass of 22.88 gL −1 on PDA medium at 25 • C after 6 days [33]. This biomass was lower than that produced by the fungus Talaromyces flavus BC1 in the same culture conditions. However, the pH and time affected the biomass production when culturing in PDB medium at 25 • C for 6 days, according to the reduction trend.

Filamentous Propagation of Fungal Strains
To evaluate growth ability, Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3 fungal strains were cultured on PDA medium plates at 25 • C for 7 days.
On the PDA medium plates, the Penicillium simplicissimum CN7 strain grew into a thin hyphal cell wall, with short and cottony mycelium. The Penicillium simplicissimum CN7 strain was initially white and became green-brown. Then, it changed to a gray-brown after 7 days of culture. The Penicillium simplicissimum CN7 strain had a rapid growth rate, reaching 34.4 µm/h (Figure 3 and Table 3).  Similarly, Talaromyces flavus BC1 rapidly grew into the thin hyphal cell wall, with long, straight, and cottony mycelium. Initially, the Talaromyces flavus BC1 strain was white, and then it became yellow after 10 days. The growth rate of the Talaromyces flavus BC1 colonies on agar plates reached 43.88 µm/h ( Figure 3 and Table 3).
The Trichoderma konilangbra DL3 strain formed a thin hyphal cell wall with short mycelium on the PDA medium. The mycelium of the strain Trichoderma konilangbra DL3 was initially white and became bright green. Trichoderma konilangbra DL3 had a fast growth rate of 106.02 µm/h (Figure 3 and Table 3).

Antagonistic Activity against Pathogenic Fungi
An evaluation of the ability to antagonize pathogenic fungal strains was carried out to determine the effect of inoculants in agriculture. Endophytic fungi and pathogenic fungi were co-cultured on PDA plates to investigate their antagonistic abilities. Antagonistic fungal strains inhibited the growth of pathogenic fungi (Figure 4).
The results show that the fungus Trichoderma konilangbra DL3 could inhibit the fungus C. gloeosporioides (CD1), which causes anthracnose on fruit; well antagonize the fungus F. oxysporum, which causes wilt disease; well inhibit the fungus L. theobromae N13, which causes stem rot disease on mango; and weakly inhibit N. dimidiatum fungus, which causes brown spot disease on dragon fruit. Talaromyces flavus BC1 could inhibit C. gloeosporioides and is resistant to L. theobromae N13. Penicillium simplicissimum CN7 could inhibit L. theobromae N13 fungus, and it had weakly inhibitory or non-inhibitory effects on other fungi.  [43] reported that Trichoderma viride had an antioxidant response to Aspergillus niger, which causes collar rot in groundnut. In another study, Trichoderma viride was reported to inhibit the growth of the mycelium of Sclerotium rolfsii, Fusarium solani, and Rhizoctonia solani. In addition, the alcohol extract of the mycelium showed strong antibacterial activity against Bacillus subtilis, E. coli, and Pseudomonas fluorescens, and these substances also showed significant antifungal activity against Candida albicans, Fusarium oxysporum, Rhizoctonia solani, and Fusarium solani. Furthermore, Ribeiro et al. (2018) [44] found that Diaporthe sp. and Curvularia sp. inhibited Gram-negative bacteria that cause disease with Gram-positive bacteria, such as Staphylococcus epidermidis, Pseudomonas aeruginosa, Staphylococcus aureus, and E. coli. Iswarya and Ramesh (2019) reported that Penicillium dalea EF4 isolated from the seaweed Enteromorpha flexuosa Linn showed antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa [45].
In a study conducted Ramalingam et al. (2014) [46], Penicillium spp. isolated from legume roots could produce amino acids. Strains RDA01, NICS01, and DFC01 fostered the growth of cycad. Furthermore, NICS01 minimized salinity and protected sesame plants from Fusarium infection. The results of this study suggest that NICS01 can be used as a biofertilizer and a biocontrol agent, as it enhances plant growth under biotic and abiotic stress conditions. Therefore, the application of NICS01 is extremely favorable for crop cultivation, even in saline environments.
In addition, the results of previous studies have shown that Penicillium spp. can prevent Fusarium infections in crops [47,48]. An evaluation of the feasibility of secreting multiple antibiotics, such as 2,4-diacetylphloroglucinol, pyoluteorin, pyrrolnitrin, pyocyanin, oligomycin, and phenazine, by using biological control agents that can inhibit pathogenic fungi was carried out [49].
In another study conducted by Farias et al. [50], plant-growth-stimulating fungi, such as Trichoderma Asperella, Pochonia chlamydosporia, Purpureocillium lilacinum, Metarhizium anisopliae, and Beauveria bassiana, were applied to maize, sugarcane, soybean, and tomato crops, which were grown under two conditions: with and without a fungi complex.
Using fungi as biocontrol agents is extremely beneficial due to their diversity and metabolic efficiency, and it enhances the chances of finding isolates for environmentally friendly biological control because they are mainly decomposers [51]. The fungi of the genera Aspergillus, Fusarium, Gliocladium, Petriella, and Trichoderma are known to be important biocontrol agents [52]. The biocontrol activity of Verticillium leptobactrum against green wilt caused by Fusarium oxysporum and F. lycopersici was demonstrated by Hajji-Hedfi et al. [53].

Materials
The fungal strain Penicillium simplicissimum CN7 was isolated from the rhizospheres of Stevia rebaudiana (Bert.) Hemsl. (Hung Yen and Vinh Phuc provinces, Vietnam), Talaromyces flavus BC1 was isolated from the roots of Polyscias fruticosa (Nam Dinh and Lao Cai provinces, Vietnam), and Trichoderma konilangbra DL3 was isolated from the roots of Angelica dahurica (Ninh Binh, Phu Tho, Lao Cai provinces, Vietnam). They are phytopathogenic fungi.

Isolation of Endophytic Fungal Strains
Root isolation: Rinse the sample under running water for 5 min. When the surface of the sample is clean, soak the sample in 70 • alcohol for 1 min. Allow the sample to dry in a sterile incubator. Using sterile tools, cut the root samples into small pieces 5-10 mm long, and place them on a Petri dish with an isolation medium; then, transfer them to an incubator at 30 • C for 48-72 h.
Soil sample isolation: Take a small amount of soil, put it into a test tube containing 9 mL of sterile distilled water, and shake well. Pipette a small amount of the solution into a Petri dish with an isolation medium, and then transfer it to an incubator at 30 • C for 48-72 h.

Determination of Phosphate-Solubilizing Activity
Cultures of endophytic fungi were grown on PVK medium (10 g glucose; 5 g Ca 3 (PO 4 ) 2 ; 0.5 g (NH 4 ) 2 SO 4 ; 0.2 g KCl; 0.1 g MgSO 4 7H 2 O; 0.002 g MnSO 4 ; 0.002 g FeSO 4 ; 0.5 g yeast extract; pH 6.8-7.0) for 7-10 days at 25 • C in a shaker at 200 rpm. Post-fermentation fluid was centrifuged at 6000 rpm for 20 min to obtain cell-free supernatants. The phosphorus content in the supernatant was determined using a photometric method after the citrate radicals decomposed [57]. The photometric method employs UV/VIS/NIR spectroscopy, a powerful analytical technique used to determine the optical properties (transmittance, reflectance, and absorbance) of liquids and solids. This measurement is used to determine the amount of an analyte in a solution or liquid.

Analysis of Indole Acetic Acid Content
To determine the content of IAA produced by the endophytic fungi, a colorimetric technique was performed with Van Urk Salkowski reagent using Salkowski's method following Brick et al. (1991) [58]. Endophytic fungi were grown in medium to assess the ability to grow IAA promoters (20 g difco peptone; 1.15 g K 2 HPO 4 ; 1.5 g MgSO 4 ; 1.5% (v/v) glycerol; 0.1 g tryptophan), and they were incubated at 200 rpm for 48 h at 30 • C. Post-fermentation fluid was centrifuged at 5000 rpm for 10 min after incubation. The supernatant was used to determine the IAA content in the sample. Then, 1 mL of sample solution was added to 2 mL of Salkowski reagent (2% of 0.5 M FeCl 3 in 35% HClO 4 solution) and kept in the dark. The optical density (OD) was recorded at 530 nm after 30 min. The color of the post-reaction mixture changed from light pink to red, depending on the IAA content in the culture solution. For the development of a standard curve, the above procedure was followed with a known concentration of IAA.

Morphological and Growth Characteristics
The fungi were grown on PDA medium plates (5 g potato, 5 g peptone, 20 g glucose; pH 6.5-7.0) at 25 • C. From days 2 to the end of cultivation (days 7), the form, size, and color of the fungal colonies were observed and recorded.
The growth rate of the mycelia was determined according to the method described by Schwantes and Salttler (1971): V = ∆X/∆T, where V is the growth rate of the mycelia (µm/h); ∆X is the radius of the colony (µm); and ∆T is the cultivation time (hours) [59].

Determination of Antifungal Activity
The antifungal activity of the 3 endophytic fungi against XB1 (L. thebromae N13), CD1 (C. gloeosporioides), Fu (F. oxysporum), and DNTL (N. dimidiatum) was determined by modifying the dual-culture plate antagonism. They were cultured on PDA plates at 25 • C for 7 days in the dark. Then, endophytic fungi and pathogenic fungi strains were co-cultured in PDA medium plates by being placed symmetrically on each side of the Petri dish and being incubated at 25 • C. The growth of the fungi strains was observed and recorded regularly every day until the antagonistic fungi strains stopped growing. The experiment was repeated three times and averaged (Zhang et al.) [60].

Molecular Identification
The three fungal strains Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3 were identified using the molecular biology method. The genomic DNAs of the fungal strains were isolated for PCR amplification, and the amplified PCR products contained bands 800 in length, similar to the size of the internal transcribed spacer gene region. They were compared with the homologous nucleotide sequences on GenBank using the BLAST SEARCH program (https://blast.ncbi.nlm.nih.gov/Blast.cgi) (accessed on 15 July 2021).
The genomic DNAs of the fungi Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3 were extracted using the alkaline extraction method [61], and they were subjected to PCR to amplify the 5.8S rDNA gene using two primers: BF (5 -CTTGGTCATTTAGAGGAAGTAA-3 ) and BR (5 -CAGGAGACTTGTACACGGTCCA-3 ) [62]. The thermal cycling program was as follows: initial denaturation at 95 • C for 5 min, followed by 30 cycles; denaturation at 95 • C for 90 s; primer annealing at 55 • C for 90 s; extension at 72 • C for 2 min; and a final extension step of 72 • C for 8 min. The PCR reaction products were examined by electrophoresis in 1% (w/v) agarose gel, and the bands were stained with ethidium bromide. The PCR products were sequenced by Axil Scientific Pte., Singapore. The sequences of the fungi were compared with similar sequences from GenBank using the BLAST program to identify fungal species. The fungal ITS sequences of Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3 in this study were deposited in GenBank under accession numbers OL795992, OL795993, and OL875113, respectively.

Conclusions
Endophytic fungi strains Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3 have the ability to inhibit the growth of pathogenic fungal strains, such as fungus C. gloeosporioides (CD1); which causes anthracnose on fruit; fungus F. oxysporum, which causes wilt disease; fungus L. theobromae N13, which causes, stem rot disease on mango; and N. dimidiatum fungus, which causes brown spot disease on dragon fruit. They grow significantly over a period of 5 to 6 days. The content of the insoluble phosphate (content of P 2 O 5 ) that was resolved by the three fungal strains was 341.90 1498.46, and 390.79 ppm; the content of IAA produced in the culture medium was determined to reach 49.00, 52.35, and 33.34 ppm.
Endophytic fungi have many important applications in agriculture, medicine, ecology, biotechnology, and other industries. They play a role in promoting plant growth and are environmentally friendly. Therefore, these fungi can be used as biopesticides and biofertilizers for plant growth and health and, thus, are capturing more attention. The results of this study can be used as a premise for further studies on the use of probioticcontaining rhizosphere fungi to re-infest soil in order to help support growth, increase resistance, and increase the yield of crops in general and medicinal plants in particular.