Impact of Antibiotics as Waste, Physical, Chemical, and Enzymatical Degradation: Use of Laccases

The first traces of Tetracycline (TE) were detected in human skeletons from Sudan and Egypt, finding that it may be related to the diet of the time, the use of some dyes, and the use of soils loaded with microorganisms, such as Streptomyces spp., among other microorganisms capable of producing antibiotics. However, most people only recognise authors dating between 1904 and 1940, such as Ehrlich, Domagk, and Fleming. Antibiotics are the therapeutic option for countless infections treatment; unfortunately, they are the second most common group of drugs in wastewaters worldwide due to failures in industrial waste treatments (pharmaceutics, hospitals, senior residences) and their irrational use in humans and animals. The main antibiotics problem lies in delivered and non-prescribed human use, use in livestock as growth promoters, and crop cultivation as biocides (regulated activities that have not complied in some places). This practice has led to the toxicity of the environment as antibiotics generate eutrophication, water pollution, nutrient imbalance, and press antibiotic resistance. In addition, the removal of antibiotics is not a required process in global wastewater treatment standards. This review aims to raise awareness of the negative impact of antibiotics as residues and physical, chemical, and biological treatments for their degradation. We discuss the high cost of physical and chemical treatments, the risk of using chemicals that worsen the situation, and the fact that each antibiotic class can be transformed differently with each of these treatments and generate new compounds that could be more toxic than the original ones; also, we discuss the use of enzymes for antibiotic degradation, with emphasis on laccases.


Introduction
Antimicrobials are drugs that had great success in the history of medicine; their discovery is associated frequently with the modern era after great scientists, such as Paul Ehrlich and Alexander Fleming. Ehrlich, in 1904, discovered Salvarsan; the first

Ansamycins
Contain an aliphatic chain connecting the two ends of a naphthoquinone core [24] Binds to the β subunit of RNA polymerase, inhibiting its activity [24].

Rifampicins
Rifaximin RFP X Rifampicin RIF X Rifapentine RPT X Rifabutin RFB X

β-lactams
The β-lactam ring chemically defines this class of antibiotics. This ring is bound to other radicals. The association of different types of linear chains modifies the properties of the compound and the different groups of β-lactam antibiotics are formed [17,25]. Their structure consists of a five-membered unsaturated ring fused to an β-lactam ring. Carbapenems differ from penicillins by the C2-C3 double bond and the carbon in place of the sulphur at C1. Additionally, carbapenems have a trans-1-hydroxyethyl substituent in place of the acylamino substituent on the β-lactam ring [26].
They act by two mechanisms: inhibition of wall synthesis and induction of bacterial autolysis. Transpeptidase enzymes (E.C. 3.4.16.4) are involved in the last stage of wall synthesis, linking the bonds of the peptidoglycan chains. The β-lactam ring is structurally similar to the region of the pentapeptide to which transpeptidases bind, thus the ring binds to the enzymes inhibiting cell wall formation. In addition, β-lactam activate an endogenous autolysin that degrades the peptidoglycan [17,25]. The chemical structure of cephalosporins comes from 7-cephalosporanic acid. Cephalosporins structure are a fusion of a two-ring system of -lactam-3-dihydrothiazine, known as 7-aminocephalosporanic acid (7-ACA), and vary in their side-chain substitutions at C3 (R2) and C7 [27].

Structure
Target Antibiotic

CLSI H A
The chemical structure of cephalosporins comes from 7-cephalosporanic acid. Cephalosporins structure are a fusion of a two-ring system of -lactam-3-dihydrothiazine, known as 7-aminocephalosporanic acid (7-ACA), and vary in their side-chain substitutions at C3 (R2) and C7 [27].
They act by two mechanisms: inhibition of wall synthesis and induction of bacterial autolysis. Transpeptidase enzymes (E.C. 3.4.16.4) are involved in the last stage of wall synthesis, linking the bonds of the peptidoglycan chains. The β-lactam ring is structurally similar to the region of the pentapeptide to which transpeptidases bind, thus the ring binds to the enzymes inhibiting cell wall formation. In addition, β-lactam activate an endogenous autolysin that degrades the peptidoglycan [17,25]. The basic structure of penicillin (6-aminopenicillanic acid) consists of a thiazolidine ring, an attached p-lactam ring and a side-chain [17,25].
They act by two mechanisms: inhibition of wall synthesis and induction of bacterial autolysis. Transpeptidase enzymes (E.C. 3.4.16.4) are involved in the last stage of wall synthesis, linking the bonds of the peptidoglycan chains. The β-lactam ring is structurally similar to the region of the pentapeptide to which transpeptidases bind, thus the ring binds to the enzymes inhibiting cell wall formation. In addition, β-lactam activate an endogenous autolysin that degrades the peptidoglycan [17,25].
Ampicillin AM X AMC X X

Glycopeptides and Lipoglycopeptides
Consist of a central heptapeptide core to which amino acid residues and sugars are attached [29].
In Gram-positive bacteria, Glycopeptides and lipoglycopeptides bind to the D-alanyl-D-alanine terminus at the carboxy-terminal end of bacterial wall precursor peptides, thus blocking peptidoglycan synthesis [24,29].

Glycyliclinas
Structurally similar to the tetracyclines, it has a central structure of four carbocyclic rings, with a t-butylglycylamide substitution at position 9 of the minocycline that confers a broad spectrum of activity [24].
Inhibits protein synthesis by reversibly binding to the 30S subunit of the bacterial ribosome, blocking the entry of the aminoacyl tRNA into the A site of the ribosome, thus preventing amino acid incorporation and subsequent elongation of peptide chains [24].
Tigecycline TGC X Lipopeptides A cyclic molecule with 13 amino acids, ten of which are part of the cyclic structure, and the remaining three make up a side chain with an N-decanoyl residue [24].
They insert into the membrane bilayer causing its depolarisation, with a strong loss of potassium ion leading to cell death; a side-chain bearing an N-decanoyl residue [24].
Daptomycin DAP X Colistin CL X Polymyxin B PB X X

Macrolides
Are a lactonic ring with 14 to 16 carbons, bound to an aminated sugar [24].
Binds to sequences of the 23S rRNA domain V, which is part of the 50S subunit, preventing elongation of the peptide chain by blocking the polypeptide exit tunnel and thus dissociating the peptidyl-RNA complex from the ribosome [24].
Azithromycin AZM X X Are a lactonic ring with 14 to 16 carbons, bound to an aminated sugar [24].
Binds to sequences of the 23S rRNA domain V, which is part of the 50S subunit, preventing elongation of the peptide chain by blocking the polypeptide exit tunnel and thus dissociating the peptidyl-RNA complex from the ribosome [24]. Oxazolidinone consists of a ring with three carbon atoms, an oxygen atom at position one and a nitrogen atom at position three [30].
The antibiotic binds to the 50S subunit, affecting protein synthesis, and also inhibits the initiation complex by binding to the 70S subunit. The D-ring of the tedizolid contributes to the presence of additional hydrogen bonds that provide further interactions between the tedizolid and the bacterial ribosome; therefore, the drug is more potent [30].

Phosphomycins
It is cis-1,2-epoxypropylphosphonic acid (-), a simple, water-soluble molecule with only three carbon atoms and no nitrogen. The carbon atom is bonded to the phosphorus atom without an intermediate oxygen bridge. The antimicrobial activity is due to the epoxy bond [24].
Fosfomycin FOS X X
Quinolones and fluoroquinolones interfere with DNA synthesis, inducing cell death. They penetrate the wall through porins and interact with two enzymes; DNA gyrase (E.C. 5

Lincosamides
Lincomycin consists of an amino acid attached to an amino sugar; clindamycin differs structurally due to the substitution of a chlorine atom by a hydroxyl group and the inversion of carbon [24].
It binds to sequences of rRNA 23S domain V, which is part of the 50S subunit of the ribosome, preventing the elongation of the peptide chain by blocking the polypeptide exit tunnel, and thus the peptidyl-RNA complex dissociates from the ribosome [24].

Pseudomonic Acids
It consists of a 9-hydroxy-nonanoic acid chain, some spatial similarity in structure to the amino acid isoleucine [31].
Mupirocin MUP X

Rhinophenazines
It contains a phenazine core with an alkyl imino (R-imino) group at position two and phenyl substituents at positions 3 and 10 of the phenazine core. The alkyl imino group is essential for antimicrobial activity [33].
Its target is the bacterial respiratory chain and ion transporters. Intracellular redox cycling, involving oxidation of reduced clofazimine, generates antimicrobial reactive oxygen species (ROS) and hydrogen peroxide (H 2 O 2 ). Additionally, clofazimine interaction with membrane phospholipids promotes membrane dysfunction and interferes with K + uptake. Both mechanisms result in interference with cellular energy metabolism by disrupting ATP production [33].
Inhibits protein synthesis by preventing translocation of elongation factor (EF-G) during protein synthesis [34].
Fusidic acid FUS X

Sulphonamides
They have a similar structure to para-aminobenzoic acid. Contain an aromatic NH 2 substituent at the para position of the benzene ring. Contain a substituent at the ortho-and meta-position of the benzene ring. Additionally, it contains a double substituent at position N1 and a sulphonamide group on the benzene ring [24]. Within the critical importance category, aminoglycosides (Table 1) are commonly used in clinical practice. Various resistance mechanisms have been reported; however, these compounds are active against most aerobic Gram-negative bacilli, as well as the β-lactams. Due to their polycationic nature, aminoglycosides are highly soluble in water and exhibit low gastrointestinal absorption, resulting in their excretion into the environment. Additionally, due to their chemical structure, these antibiotics are stable. The presence of these compounds in water and soil causes ecotoxicity; for example, Streptomycin (STR) residues are toxic to algae [36]. Wollenberger et al. (2000) performed biotoxicity tests of Streptomycin (STR) against Daphnia magna, obtaining a value of the median effective concentration of about 48 h EC50 = 487 mg L −1 [37]. Furthermore, Streptomycin (STR) resistance genes have been identified in pathogenic bacteria, such as Salmonella spp., and Enterococcus spp., [38,39]. In addition to aminoglycoside's ecotoxicity, they can cause adverse effects in humans because they are ototoxic and nephrotoxic, and cause neuromuscular toxicity (less frequently), toxicity in the haematopoietic system, and also allergic reactions (rash, fever, angioedema or exfoliative dermatitis). However, the risk of toxicity depends on different factors, such as pre-existing diseases, the severity of the disease, concomitant drugs, and genetic predisposition [40,41].
Ansamycins are considered critically important, these antibiotics are used as sole or limited therapy against tuberculosis. Rifampicins serve to treat several types of mycobacterial infections, such as Mycobacterium avium complex, and leprosy, and combined with other antibacterials, allow for treating latent or active tuberculosis. Up to 50% of this antibiotic class is excreted in faeces, affecting the environment. This antibiotic class has been found in water, and it is known for its cytotoxic effects [21,42,43].
β-Lactam antibiotics also fall into this category. They make up the majority of antibiotics in human use in most countries and account for approximately 50 to 70% of antibiotics used [44]. Compared to other compounds, they are not stable in the environment as the β-lactam ring can be degraded by β-lactamase (E.C 3.5.2.6), a common enzyme in several bacteria, and can also be degraded by chemical hydrolysis [44]. Despite the above, the presence of β-lactams and β-lactamase (E.C 3.5.2.6) in wastewaters, responsible for bacterial resistance to these antibiotics, has been demonstrated [45]. For example, Hoelle et al. (2019) isolated 332 E. coli strains in which 65 (19.6%) were resistant to carbapenems [46]. Toxicity bioassays by Havelkova et al. (2016), evaluated the toxicity of Penicillin G (PG) against Daphnia magna, obtaining values of 48 h EC50 = 1496.9 mg L −1 [44]. On the other hand, the adverse effects of penicillins and cephalosporins are hepatotoxicity, and can cause neutropenia and encephalopathy; these antimicrobials also cause neuronal excitation because the β-lactam ring is structurally similar to the neurotransmitter GABA inhibitor, causing inhibition of the gamma-aminobutyric acid (GABA) system [47]. Among the β-lactams are the cephalosporins, a broad-spectrum antibiotic class used against Gram-positive and Gram-negative causing infections. According to their activity and spectrum, cephalosporins are classified into five generations [48]. Cephalosporins are useful for both humans and animals, and approximately more than 50% of these drugs are eliminated structurally unmodified through the urine [49]. Cephalosporins are the most produced, prescribed, and consumed class of antibiotics in Europe, and their presence in wastewaters represents a problem, as they contribute to increased COD (Chemical Oxygen Demand); increasing remediation costs [48]. High concentrations of cephalosporins have also been reported ranging from µg L −1 to mg L −1 [50,51]. Penicillins are broad-spectrum β-lactams for infectious diseases treatment in humans and animals; however, the emergence of multiresistant β-lactamase (E.C 3.5.2.6) or penicillinase (E.C 3.5.2.6) producing bacteria has limited the use of penicillins in recent years. Penicillins are still recommended and widely used by physicians [52], but a dose of penicillin is generally excreted through the urine without structural changes; however, they could be transformed [53]. The environmental presence of these compounds has been found in concentrations of about 1 to 1000 µg L −1 in aquatic environments [54]. Penicillins are ecotoxic; the presence of penicillins in untreated effluents can cause the death of Daphnia magna [55].
On the other hand, glycopeptides are antibiotics considered of critical importance and detected in high concentrations in wastewaters [56,57], generating a harmful environmental impact. Havelkova et al. (2016) developed biotoxicity bioassays for vancomycin, obtaining against D. magna, values of 48 h EC50 = 686.9 mg L −1 [44]. The presence of glycopeptides in aquatic environments represents a problem due to antimicrobial resistance. For several years, different pathogenic species of the genus Enterococcus have been isolated from livestock farms and classified as resistant to Vancomycin (VA) and Teicoplanin (TEC). Likewise, other bacteria causing infections in humans, such as Staphylococcus aureus, have unfortunately acquired resistance to commonly used antibiotics, including Vancomycin (VA) [58].
Tigecycline (TGC), a critically important antibiotic, is used as a limited therapy for infections caused by multidrug-resistant Enterobacteriaceae and methicillin-resistant Staphylococcus aureus [21,22,43]. It is used to treat infections, such as community-acquired pneumonia, complicated intra-abdominal infections, and complicated skin infections [59]. 59% of the Tigecycline (TGC) dose is excreted in the biliary/faecal matter, and 33% is excreted in the urine; 22% of the drug is structurally unchanged; which has increased bacterial resistance to Tigecycline (TGC). The presence of the TetX gene is responsible for Tigecycline (TGC) resistance and has been detected in aquatic environments and wastewaters treatment systems [60].
Lipopeptides are of critical importance to antibiotics; especially the use of polymyxins decreased due to their adverse effects (nephrotoxicity); however, because of the emergence of multi-resistant Gram-negative bacteria and the lack of new antibiotics, they are increasingly used as a last-line treatment against these pathogens. According to Zurfuh et al. (2016), there is no literature about polymyxins detection in water, attributed to the lack of extraction methods; however, bacteria with polymyxin resistance genes have been found in wastewaters, surface water and groundwater [61][62][63].
Macrolides are also of critical importance and are a limited therapy against Legionella spp., Campylobacter spp., multidrug-resistant Salmonella spp., and Shigella spp., [21,22,43,64]. Macrolides have high activity against Gram-positive and Gram-negative bacteria in humans and animals. Up to 50% of macrolides are excreted in faeces; they have been detected in water (varying concentrations) [65,66], and their cytotoxic effects have been proven [67].
Oxazolidinones are of critical importance and a limited therapy against multidrugresistant Staphylococcus aureus and Enterococcus spp., infections [21,22,43]. They are also considered "last-resort" antibiotics, which are important against Gram-positive bacteria resistant to β-lactams and glycopeptides. However, resistance genes have been detected in pathogens, such as Enterococcus spp., and Clostridium perfringens [68,69]. In addition, the toxicity of this class of antibiotics and their degradation products has been demonstrated [70,71].
Fosfomycin (FOS) also belongs to antibiotics of critical importance and essential medicines [72]. It is used as a limited therapy against extended-spectrum β-lactamase (E.C 3.5.2.6) (ESBL)-producing E. coli, which causes urinary tract infections [21,22,43]. In poultry, it is used in infectious diseases, because it is effective against bacteria, such as E. coli, Klebsiella spp., Proteus mirabilis, Staphylococcus saprophyticus, Enterococcus spp., and Streptococcus agalactiae [73]. Fosfomycin (FOS) is excreted without structural change approximately 38% in urine, while 18% in faeces [74]. The presence of Fosfomycin (FOS) in wastewaters from the pharmaceutical industry and the presence of fosfomycin resistance genes have been reported. The high concentrations of FOS make them recalcitrant and toxic to the environment, as they can trigger eutrophication leading to water pollution and nutrient imbalance [75][76][77].
Quinolones are considered critically important and used as a limited therapy against Campylobacter spp., the invasive disease caused by Salmonella spp., the multidrug-resistant Salmonella spp., and Shigella spp., [21,22,43,64]. They are used for the treatment of diseases in both humans and animals. Due to their high adsorption potentials, non-degraded compounds in the treatment plants are adsorbed into the sludge in small fractions; subsequently being released into the environment. Molecules, such as Ofloxacin (OFL), Ciprofloxacin (CIP), Nalidixic acid (NAL), Norfloxacin (NX), and resistance genes have been detected in wastewaters. The toxicity of these compounds and adverse effects in humans have also been demonstrated, with gastroenteritis and joint pain being the most common effects [78][79][80][81][82].
Phenicols are broad-spectrum antibiotics for infectious disease treatment in both humans and animals. It is a high importance antibiotic class [21,22,43]. In aquaculture, Florfenicol (FLO) and Chloramphenicol (CHL) are frequently used although their veterinary use is restricted [83]. Furthermore, this antimicrobial can be excreted without structural changes, increasing its presence in the environment.
On the other hand, lincosamides are a highly important antibiotics class, used against infections caused by Enterococcus sp., and Staphylococcus aureus. Due to the reduced susceptibility of S. aureus to β-lactam antibiotics, the clinical use of macrolides and lincosamides against penicillin-resistant S. aureus has been promoted [84]. Bruyndonckx et al. (2021) reported that lincosamides were among the most consumed classes in Sweden and accounted for more than 20% of macrolides, lincosamides and streptogramins consumption in Austria, Finland, Germany, Hungary, and Luxembourg between 1997 and 2017 [52]. Lincosamide resistance genes have been reported in pharmaceutical industry wastewaters treatment plants [85].
Pseudomonic acids have a unique chemical structure and mode of action, are highly relevant, have a broad spectrum of activity against Gram-positive bacteria, and exhibit "in vitro" activity against some Gram-negative bacteria [86]. They are mainly used to treat impetigo caused by Staphylococcus aureus and Streptococcus pyogenes; however, mupirocinresistant S. aureus has already been reported [86].
Riminophenazine (RPZ) is a high importance antibiotic for treating dapsone-resistant lepromatous leprosy and lepromatous leprosy complicated by erythema nodosum leprosum; Riminophenazine (RPZ) is used in combination with other compounds, such as dapsone. Clofazimine (CFZ) is an antimicrobial dye that produces the discolouration of skin and body fluids and has "in vitro" activity against mycobacteria, such as Mycobacterium tuberculosis. However, it is ineffective compared to classical anti-TB treatments, such as Rifampicin (RIF) and Isoniazid (INH). After a dose of 300 mg, the concentration in urine and faeces is negligible Holdiness [87].
Fusidic acid (FUS) is also of high importance antibiotic [21,22,43], used topically to prevent and treat skin infections. It is widely used in Europe and has activity against Gram-positive bacteria. The resistance of S. aureus strains to Fusidic acid (FUS) has been also demonstrated [88].
Sulphonamides are also of high importance [21,22,43], with a broad spectrum used for the treatment of infectious diseases in both humans and animals (in combination with other compounds). Some time ago, these antibiotics were widely used in humans; however, their adverse effects and the emergence of resistant bacteria, have led to restrictions on their human use; in contrast, are widely used in pigs and cattle. The main releasing route for these antibiotics into the environment is farm wastewaters. This class of compounds has been detected in effluents from treatment plants, surface water, and groundwater. Genes conferring resistance to these antibiotics have also been identified [89,90].
Tetracyclines are of high importance main class of antibiotics against infectious diseases in humans and livestock. They are broad-spectrum and low-cost antibiotics, but their hydrophilic nature, favour more than 75% of the dose being excreted, without structural changes [91]. These antibiotics have been detected in water, sludge, and various aquatic environments combined with cations (Ca 2+, Mg 2+ y Cu 2+ ) [92]. Sixty-five resistance genes of first-and second-generation tetracyclines are distributed among 130 species of Gram-negative and Gram-positive microorganisms, including Mycobacterium spp., and Streptomyces spp., [93].

Antibiotic Use in Livestock Farming
Antibiotics are frequent in the livestock industry to prevent and treat diseases caused by bacteria, allowing for healthy growth and reduced animal mortality and morbidity. The use of these antibiotics occurs principally in pigs, cattle, poultry, and the aquaculture industry [5,94] Livestock farmers use antibiotics for metaphylactic, prophylactic, and growth promotion purposes. Metaphylaxis is the administration of drugs to presumably infected or disease susceptible groups of animals to treat and control the disease transmission among animals in close contact. Prophylaxis is the administration of drugs to a group of animals before clinical signs of a disease to prevent its occurrence [95]. Antibiotics also can be used as growth promoters; the destruction of the intestinal microbiota generates increased assimilation of the feed consumed; therefore, an increase in the muscle mass of the cattle occurs fewer times; however, this practice has been banned in some countries [11].
According to a report by the FDA between 2009 and 2018, the annual amount of antibiotics used in animals reached 8,000,000 Kg, with tetracyclines being the most widely used [7]. Global consumption of antimicrobials used in food-producing animals was estimated at 63,151 tonnes (http://www.fao.org/antimicrobial-resistance/key-sectors/ animal-production/en/ accessed on 12 April 2022).

Antibiotics Use in Livestock Farming and Foodborne Diseases (FBD)
According to the WHO, Foodborne Diseases (FBD) are diseases commonly transmitted through the ingestion of contaminated food. FBD involves a broad group of illnesses caused by microbial pathogens, parasites, chemical contaminants, or biotoxins [96]. The severity of these diseases in humans varies from mild to severe symptoms, which require lifelong treatment. It has been estimated that, in industrialised countries, more than 10% of the population could suffer from a disease associated with the ingestion of contaminated food [97]. One of the aspects that increase the severity of FBD is the presence of antibioticresistant microorganisms, leading to therapeutic failure in humans. This resistance is directly associated with the use of antimicrobials for prophylaxis, metaphylaxis, or as growth factors during livestock destinated to a source of meat, eggs or milk [98].
In animal food production, the use of antibiotics has increased; for example, 63,151 tons of antibiotics were used in livestock production in 2010, and have been estimated that there will be a 67% increase by 2030 [99].
The scenario tends to be worsened by manure used as a crop fertiliser, leading to increased dissemination of bacteria and antibiotic resistance genes. Disseminated bacteria and resistance genes will have contact with the soil microbiome, which could lead to loss of biomass and a reduction in nitrification, denitrification, and respiration activity, as well as impairment of enzyme activity, such as dehydrogenases (E.C. 1. The exact amount of antibiotic use in animal production is difficult to define; however, it is higher than hospital use [99,100]. Pathogen entry into the meat supply chain occurs at any stage of production (rearing, processing, distribution, sale, handling, and preparation) [101]; some authors have described the slaughter stage, during the removal of the gastrointestinal tract or intestinal contents, as the principal source of pathogen dissemination by cross-contamination through equipment, utensils and personnel [101][102][103][104][105], increasing the risk of contamination at the production line, considering the ability of some of the microorganisms (Enterobacteria) to generate biofilms [106].
The entry of antibiotics into the meat supply chain occurs in feeding operations, where concentrates contain antibiotics used as growth promoters. However, some EU countries have prohibited antibiotics use as growth promoters (since 2006) because they applied for prophylaxis or metaphylaxis [99,100].
On the other hand, in the meat processing stage, antimicrobial resistances are transmitted through the entry of food contaminated with resistant microorganisms or through the food processing environment. Mentioning a couple of microorganisms, Salmonella spp., and Listeria monocytogenes are two of the most important bacterial agents in FBD.
Salmonella spp., (one of the pathogens transmitted during the slaughter of livestock) is the causing agent of salmonellosis, a disease that has a high morbidity rate in both industrialised and developing countries; causing acute enterocolitis with abdominal pain, bloody or non-bloody diarrhoea, nausea, and vomit [64,106,107]. Transmission of nontyphoidal Salmonella to humans can occur zoonotically through contact with faecal material from carrier animals or by the consumption of contaminated food [64,[106][107][108][109].
On the other side, L. monocytogenes (one of the pathogens transmitted during the production of processed foods) is the causative agent of listeriosis, an invasive disease; therefore, needs to cross through the intestinal barrier to gain access to internal organs, the entry takes place through Peyer's patches by M-cells. Subsequently, the bacterium goes to the liver and may develop granulomatous hepatitis, due to the invasion of hepatocytes. Afterward, the bacterium is internalized, and, in some cases, there is intracellular proliferation and dissemination to other tissues, with tropism for the Central Nervous System (CNS) and the pregnant uterus [110][111][112][113][114].
In developing countries, the main route of contamination is through consumption of contaminated vegetables, contaminated water and human-human contact, whereas in industrialised countries, the major route of contamination is related to ingestion of contaminated food products of animal origin, especially fresh meat and eggs [64,106].
The final food consumer comes into contact with antibiotic-resistant microorganisms through their presence in ready-to-eat meat, dairy and vegetable products [115]. Contact with microorganisms carrying antibiotic resistance genes can lead to two possible scenarios: (i) the modification of the gut microbiome [100], (ii) a potential hazard to human medicine due to the inability to manage infections in the general population, resulting in prolonged broad-spectrum antibiotic treatments, which would end up in a constant loop between resistances going into the environment and the population [99,100].

Waste from the Livestock Industry
According to the United States Department of Agriculture (USA), animals confined for food production generate approximately 335 million tonnes of waste per year, exceeding 40 times the mass of biosolids generated by humans [5,8].
The principal cause of bacterial resistance to antibiotics is the disposal of antibiotics in livestock waste in soil, ground and surface water, atmosphere and crops [5,9]. When livestock wastes are disposed of, the antibiotics in faeces are often broken down due to oxidation-reduction reactions, hydrolysis, biodegradation and photodegradation, reducing the concentration of the antibiotic and thus its ability to kill bacteria [116]; which generates a problem, as bacterial exposure to sub-lethal concentrations; favouring the adaptation and proliferation of bacteria with multidrug-resistant phenotypes. Whereas a high proportion of excreted antibiotics are bioactive, consequently, and due to fast bacterial reproduction, the resistance phenotype can easily express Sarmah [8].
Bacteria can resist antibiotics through several mechanisms; through physiological adaptation, bacteria can change membrane transport pumps (porins), excluding harmful agents; however, this mechanism is limited Silbergeld [5]. Another mechanism is mutations, which can alter or eliminate the target of the antibiotic [116], mutations could occur in DNA sequences that modify the production or structure of the enzymes responsible for inactivating the antimicrobial agent; for example, β-lactamases (E.C. 3.5.2.6) can inactivate the β-lactam ring, which is part of the structure of β-lactam antibiotics (Table 1). These enzymes break the amide bonding of the ring, and the generation of acidic derivatives that do not have antibacterial properties occurs by preventing the antibiotics from binding to bacterial carrier proteins [117].
Resistance genes can be shared between bacteria by three different mechanisms (transformation, conjugation, and transduction), thus transferring resistance between pathogenic and non-pathogenic bacteria, this transfer is called "horizontal transfer" and can occur between bacteria of different genera. Vertical transfer occurs during cell division through the genetic material transmission from mother to daughter cell [118].
Conjugation is one of the most effective mechanisms for genetic material transfer and is considered one of the reasons for antibiotic resistance. Conjugation is frequent in Gram-negative bacteria and to a lesser extent in Gram-positive bacteria [119,120]. During the Gram-negative conjugation process, a donor bacterium transfers a plasmid containing resistance genes to a recipient bacterium throughout sexual pilis [120,121]. During the Gram-positive conjugation process, the receptor cells excrete an inducing peptide called pheromones or autoinducers. When a certain level of peptide concentration is reached the donor cell activates the transcription of genes related to the production of aggregation substances, including adhesins and coupling proteins. The donor cell attaches to the receptor cell, which generates a channel to transfer the genetic material [122].
The transduction process, allows resistance genes to be transferred from one bacterium to another mediated by a bacteriophage that has previously undergone lytic cycling in the "donor strain".
The transformation process occurs when a bacterium incorporates DNA from the environment favoured by the state of competence generated during cell growth [116]. Furthermore, mobile genetic elements, such as plasmids, insertions sequences, transposons, and integrons are responsible for 95% of antibiotic resistance [5].
Bacterial resistance to antibiotics implies the treatment's failure against infections, the latency of infectious processes, progression of the disease to chronic processes, the transmission of the infection to other animals, outbreaks, economic losses, and even adverse reactions to antibiotics [11]. In many cases, crops for human consumption are fertilised with animal faeces and irrigated with water sources contaminated with antibiotics, resulting in the spread of resistant strains to humans and animals. Spread generates a public health problem, as treatment options against pathogenic bacteria are limited [12].
In the last 20 years, the presence of antibiotics has been documented in different water sources; surface, ground, and oceanic waters [15,123]. In addition to water sources, food contaminated with antibiotics can cause adverse allergic reactions in hypersensitive individuals [11]. Ramírez et al. (2012) administered six antibiotics intramuscularly and intramammary to 115 cows and found that 65.2% of cows had Oxytetracycline (OXT), 58.8% of cows had Tylosin (TLY), 69.2% of cows had Spiramycin (SP) and 65% of cows had Amoxicillin (AMC) traces in milk [124].
The presence of antibiotics in water is a problem for ecosystems; Xu et al. (2019) demonstrated the toxicity of Tetracycline (TE) and its degradation intermediates (anhydrotetracycline and epitetracycline hydrochloride) in microalgae. At high concentrations of Tetracycline (TE) (>5 mg L −1 ), the permeability of microalgae is affected, showing structural changes and increasing reactive oxygen species (ROS) activity in the water [125].

Antibiotic Use by Humans
Humans consume a large variety of antibiotics [95], and the consumption rate per class differs between countries; according to the WHO antibiotic consumption monitoring report, in 2015, Mongolia was the highest consumer of antibiotics among East and South-East Asian countries, reaching approximately 65 defined daily doses (ddd) per 1000 population. In this country, the highest consumption involved penicillins and other β-lactams. Other countries in the East and South-East Asia region also participated in the surveillance of antibiotic consumption; the Republic of Korea (27 ddd), New Zealand (23 ddd), Japan (15 ddd), Philippines (8 ddd) and Brunei Darussalam (5 ddd). Among these six countries, Amoxicillin was the most widely consumed [43]. On the other hand, in China, the percentage of human prescriptions (between 41 and 61%) exceeds the WHO recommended threshold (30%). China is considered the world's largest consumer of antibiotics for animal and human use; in 2011, the annual per capita antibiotic use was 138 g, exceeding ten times that of the United States [126]. Some authors attribute this high consumption to different factors. (i) self-medication, (ii) the large proportion of patients receiving combination therapy with multiple antibiotics, (iii) inappropriate antibiotic management in health care facilities and pharmacies (especially in rural areas), (iv) and a lack of public understanding and awareness of antibiotic use [126,127]. East and South-East Asian countries have made efforts to prevent inappropriate use of antibiotics and antibiotic resistance; however, there are still some gaps, such as the lack of guidelines for antibiotic residue disposal, lack of environmental standards and lack of monitoring databases [127].
Countries belonging to the Eastern Mediterranean region also reported antibiotic consumption; countries, such as Iran, Jordan and Sudan reported 38, 8 and 35 ddd per 1000 inhabitants, respectively. The highest consumption in 2015 was for penicillins, other beta-lactams, macrolides and tetracyclines [43].
Similarly, African countries, such as the United Republic of Tanzania, Côte d'Ivoire, Burundi and Burkina Faso reported consumption of 27, 11, 4 and 14 ddd per 1000 population in 2015, respectively. In these countries, penicillins, tetracyclines, sulphonamides and quinolones were the most consumed [43].
Countries of the European Centre for disease prevention and control implemented a surveillance system for antibiotic consumption in the primary and hospital sector. As a result, since 1997, 31 countries have usually reported consumption data expressed as ddd per 1000 population. The highest consumption has been of beta-lactams, with penicillins being the main antibiotics consumed (https://www.ecdc.europa.eu/en/antimicrobialconsumption/database/country-overview accessed on 27 April 2022). Some European countries also participated in the WHO surveillance system in 2015. Reports from countries, such as Turkey (37 ddd), Serbia (32 ddd), Greece (28 ddd), Romania (29 ddd), Montenegro (27 ddd), Italy (27 ddd), France (26 ddd), and Belgium (25 ddd) were the highest. Penicillins, other β-lactams, macrolides and tetracyclines were the most consumed [43]. Some countries in the Americas participated in the WHO report; Bolivia, Brazil, Canada, Costa Rica, Paraguay, and Peru reported consumption of 19, 23, 17, 14, 18, and 11 ddd per 1000 inhabitants, respectively, with penicillins being the most consumed, followed by other β-lactams, macrolides and quinolones [43]. The United States has a surveillance system for antibiotic consumption, with approximately 200,000 antibiotic prescriptions dispensed in retail pharmacies reported in 2020 [128].
Villalobos et al. (2013) implemented a surveillance system in 10 health institutions in Antioquia, Valle del Cauca and Bogotá. The highest consumption in ICUs was of Meropenem antibiotics (MEM); the high resistance of the Enterobacteriaceae family to third-generation cephalosporins (>25.6%), and a high percentage of methicillin-resistant (MR) Staphylococcus aureus strains were observed Villalobos [131]. Despite surveillance systems, the scope of these studies is limited, and there is no national record of antibiotic consumption in humans.
As in animals, in humans, antibiotics are not completely metabolised, and certain amounts are excreted into the environment through urine and faeces, contaminating water sources. The principal sources of human antibiotic contamination include waste from pharmaceutical companies, senior residences and hospital wastewaters [132][133][134].

Presence of Antibiotics in Wastewaters
Traces of antibiotics have long been found in surface, ground and oceanic waters [15,123,135], which has gradually increased reports on the state of contamination, the risks of antibiotics in the environment, the disposal of antibiotics and the prevalence of resistant bacteria from different sectors (aquaculture and livestock, wastewaters and treatment plants) [135,136].
The presence of antibiotics in the environment varies between regions, for example, the oral and parenteral antibiotics that account for at least 75% of total antibiotic consumption in Africa are penicillins (Amoxicillin, AMC), cephalosporins (Ceftriaxone, CRO), quinolones (Ciprofloxacin, CIP), sulphonamides (Sulfamethoxazole, SMX and Trimethoprim, SXT), tetracyclines (Doxycycline, DO), and nitroimidazoles (Metronidazole, MNZ), while in Asia the consumption of cephalosporins and quinolones is prevalent [136,137].
In Colombia, the most widely consumed antibiotics by humans are the β-lactams, mainly penicillins, such as Amoxicillin (AMC) (the most consumed by self-medication), Ampicillin (AM) and Dicloxacin (DX), some cephalosporins (Cephalexin CN, Ceftriaxone CRO, and Cephradine RAD), as well as fluoroquinolones (Ciprofloxacin (CIP)). Tetracyclines (Doxycycline DO) and macrolides (Azithromycin AZM) have also been reported [140]. The highest concentration of antibiotics in Colombia has been reported in hospital wastewaters, followed by municipal wastewaters and natural aquatic environments. Antibiotics, such as Ciprofloxacin (CIP), Azithromycin (AZM), and Sulfamethoxazole (SMX) are frequently found in Colombian wastewaters [140].
Additionally, resistant pathogens have been reported worldwide in water and clinical (nosocomial) settings; Gram-negative bacteria, such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumanni produce β-lactamases and carbapenemases and Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus or vancomycin-resistant Enterococcus sp., also produce them [43].

Global Problem of Antibiotic Resistance
The WHO has recognised antibiotic resistance as a public health threat, as these compounds are increasingly ineffective for infectious disease treatment, and clinical development of new antimicrobials is stagnating. Between 2010 and 2015 the FDA approved eight new antibiotics (Ceftaroline (CPT), Fidaxomicin (FDX), Bedaquiline (BQ), Dalbavancin (DAL), Tedizolid (TZD), Oritavancin (ORI), Ceftolozane-tazobactam, C/T, and Ceftazidime-avibactam, CZA) [141]. In 2018 and 2019, only 9/107 (8.4%) introduced drugs were approved [142]. WHO has stated that new compounds are crucial for infectious disease treatments; however, without better authority, control, and farmers and public responsibility in the use way, the new antibiotics could be ineffective. In our opinion, better control means, in addition to standards, enforcement measures, and sanctions for non-compliance, also includes trying to ensure that antibiotics approved for humans are not approved for animals as far as possible.
On the other hand, approximately a half-million new cases of rifampicin-resistant TB (RR-TB) were detected in 2018; according to the WHO, MDR-TB requires longer treatments, which are less effective and much more expensive than non-resistant TB [143].
According to WHO, the principal causes of antibiotic resistance are misuse and overuse of antibiotics, farms, lack of access to clean water, lack of hygienic conditions in both animals and humans, inadequate measures in health care facilities, limited access to medicines, non-compliance with legislation and lack of knowledge. This situation becomes critical in marginalised, poor, and health crisis regions. Antibiotic resistance rates have increased to the extent that infections caused by resistant pathogens have been treated with last-choice drugs, which may be less effective, less safe, and more expensive; these drugs are often limited and found only in developing countries [43,141].
In 2015, the WHO propitiated a global collaboration to standardise antimicrobial resistance surveillance, the Global Antimicrobial Resistance Surveillance State (GLASS), an organisation involving different countries to produce reliable and comprehensive data, which allowed the assessment of trends in antibacterial resistance between 2015 and 2019. Countries provided data on pathogens isolated from blood, urine, faeces, cervical, and urethral samples. The most frequently reported resistant pathogens were E. coli, K. pneumoniae, S. aureus, S. pneumoniae, Salmonella spp., N. gonorrhoeae, and Shigella spp. [43].
In 2017, the WHO published a list of antibiotic-resistant "priority pathogens" to guide and promote research to combat this global problem. It includes 12 genera of bacteria classified as critical, high, and medium priority. Critical priority pathogens included Acinetobacter baumannii, Pseudomonas aeruginosa, and carbapenem-resistant, ESB-producing Enterobacteriaceae. According to PAHO (Pan American Health Organisation), these microorganisms are dangerous in hospitals, senior residences, nursing homes, and patients using devices, such as ventilators and intravenous catheters. They also cause infections involving blood and lungs, urinary tract, and surgical wounds and can cause nosocomial disease, and are associated with high mortality. The high priority category includes pathogens, such as vancomycin-resistant Enterococcus faecium, methicillin-and vancomycin-resistant Staphylococcus aureus, clarithromycin-resistant Helicobacter pylori, fluoroquinolone-resistant Salmonella spp., and cephalosporin-resistant Neisseria gonorrhoeae. The medium-priority category includes Streptococcus pneumoniae with decreased susceptibility to penicillin, ampicillinresistant Haemophilus influenza, and fluoroquinolone-resistant Shigella spp.

Overviews of Degradation of Antibiotics
Antibiotics are ionisable and can be in the environment as neutral or charged (negative or positive) species. These species have different chemical properties, hence different sorption and degradation mechanisms in soil [144]. Several biotic and abiotic factors influence the degradation rate of these compounds, which is why each can have a different half-life in soil, ranging from <1 to 3466 days [145,146]. Enzymatic hydrolysis is one of the most frequent abiotic degradation pathways. The β-lactams are more susceptible to this degradation than macrolides or sulphonamides, while quinolones and tetracyclines are susceptible to photodegradation. These differences are inevitably dependent on the chemical structure of each class of antibiotics [145].
Several techniques have been assayed for the degradation and mineralisation of antibiotics to reduce the environmental impact [147]; strategies focused on physical, chemical, biological, and combined process design for degradation [147][148][149][150][151][152][153]. Physical and chemical methods include sedimentation, filtration, and oxidation using chemical compounds, UV or Vis light, ultrasound, and ions. Biological ones include the use of microorganisms and or their enzymes [148][149][150].
Unfortunately, many conventional wastewater treatment plants worldwide are not appropriate for removing highly polar micropollutants, such as antibiotics [148]. These plants usually use methods, such as filtration to remove larger solids, whereby wastewaters pass through a granular media, such as sand, charcoal, diatomaceous earth, or activated carbon; subsequently, removing the smaller solids by mechanisms, such as particle reten-tion, electrostatic attraction, or adsorption. These wastes are not degraded and concentrate in a solid phase, generating a new waste. Sometimes, these sludges with antibiotic concentrations ranging from ng kg −1 to 100 mg kg −1 are used as fertilisers, indicating that the problem has not been solved but shifts the contaminants to other environments [146]. Chemical-assisted sedimentation allows the removal of suspended solids, for which chemical compounds, such as lime (CaO), potassium aluminium sulphate (KAl(SO 4 ) 2 12H 2 O), or iron salts (FeSO 4 ) facilitate the precipitation of solids and their subsequent removal [148]. In addition, conventional wastewaters treatment plants use aerobic or anaerobic processes to remove organic matter [147,149].
Advanced Oxidation Processes (AOP) have been proposed for industrial wastewaters treatments, such as photocatalysis with TiO 2 using heterogeneous semiconductors, UV photolysis, Photo-Fenton, or electro-Fenton.
Chlorinated compounds are frequently used in drinking water treatment plants as disinfectants or oxidising agents. However, the reaction of chlorine with aromatic rings, neutral amines, and double bonds generates halogenated compounds, which may be carcinogenic [147,149].

Biotic Degradation of Antibiotics
Several authors have studied the biodegradation of antibiotics, Jiang et al. (2010) evaluated the degradation of cephalosporins in the surface water and sediments of Lake Xuanwu, China, showing that abiotic hydrolysis (for Cephradine RAD, Cefuroxime CXM, and Cefepime FEP) and direct photolysis (for Ceftriaxone CRO) were the main mechanisms of cephalosporin removal in the surface water of the lake; while aerobic and anaerobic biodegradation removed cephalosporins in the sediment [155].
The degradation of antibiotics in soil depends on physicochemical characteristics [145,146]. Antibiotics accumulate in the soil's upper layers, which act as a sink; these compounds are washed away by surface water and then leach into groundwater. The transport of antibiotics depends on the chemical properties of each antibiotic and the soil; soil organic carbon content, clay content, texture, pH, and ionic strength can alter the degree of sorption [144,156]. The sorption of antibiotics in the soil can modify their mobility, ecotoxicity, and bioavailability for microbial degradation; with low sorption, there is an increased risk of leaching and increased risk of contamination to groundwater [144,157].
Different classes of antibiotics, such as tetracyclines, sulphonamides, and macrolides, have been quantified in soils, sludge and manure. In sludge, for example, concentrations of Ciprofloxacin (CIP), Norfloxacin (NX) and Tylosin (TLS) have been reported with an average value above 1000 µg kg −1 . Other antibiotics, such as Tetracycline (TC) and Oxytetracycline (OXT) appeared in sludge with values above 10 µg kg −1 dry matter. Additionally, antibiotics in manure have been identified, with concentrations between 100 and 1000 µg kg −1 dry matter. The manure or sludge application as fertiliser can facilitate the spread of resistant bacteria in the soil, particularly human pathogenic bacteria. However, their microbial degradation has been also reported [146,[156][157][158][159][160][161][162].
Lin and Gan (2011) studied arid soils concerning the capacity of sorption and persistence of Sulfamethoxazole (SMX) and Trimethoprim (SXT). Sulfamethoxazole (SMX) sorption was minimal sorbed concentration (Cs = 0.973 µg kg −1 ), indicating a high risk of leaching, while Trimethoprim (SXT) showed moderate to high sorption (K d = 7.4242 L kg −1 ). The degradation of antibiotics depended on microbial activity, oxygen in the soil, soil type, and antibiotic characteristics [157]. Pan and Chu (2016) obtained similar results; antibiotics belonging to the sulphonamides presented low sorption measured as the adsorption or soil distribution coefficient (K d = 1.365 L kg −1 ), implying a higher risk to groundwater. In contrast, Tetracycline (TE) obtained the highest sorption (K d = 1093 L kg −1 ) value, which would mean a lower risk of groundwater contamination with leachates. Furthermore, the antibiotics evaluated were susceptible to microbial degradation under aerobic and anaerobic conditions. They also showed that, at high antibiotic concentrations, degradation processes are slowest, and their persistence in the soil is prolonged [144].
On the other hand, in a composting process of sludge or manure, antibiotic removal can range from 17 to 100% [146,163]. Composting is one of the techniques used for antibiotic and resistance gene removal in sludge or manure. The persistence and mobility of antibiotics in compost depend on their chemical properties, concentration, and physicochemical conditions, including pH, temperature, total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), and metal content. However, the removal efficiency of residual antibiotics, and antibiotic resistance genes, is still very low [163].

In Situ Chemical Oxidation (ISCO) of Antibiotics
In situ chemical oxidation is a removal strategy for different compounds, based on the addition of oxidising chemical compounds into the soil and groundwater to remove organic pollutants. Several authors have studied the degradation of antibiotics based on this strategy: thermo-activated persulfate oxidation [164], peroxymonosulfate oxidation [165], ozone oxidation [166], or ozone-based advanced oxidation process [167]. Wu et al. (2020) used a peroxymonosulfate activation support (amorphous CoSx cages advanced oxidation catalysts) to detect the degradation of Tetracycline (TE, 90%) and Ciprofloxacin (CIP, 90%). They also identified the intermediates and possible degradation routes after peroxymonosulphate activation, in which reactive species (SO 4 , O 2 , and OH) formed, and oxidised Tetracycline (TE) in reactions, such as deamidation, hydroxylation and the ring-opening reaction. They suggested that some intermediates (SO 4 · − /O 2 · − /·OH) could be mineralised (H 2 O and CO 2 ) [168]. Guo et al. (2013) used Co 3 O 4 -catalyzed peroxymonosulfate oxidation based on sulphate radicals for Amoxicillin (AMC) degradation. This process is based on the generation of sulphate radicals through Co 3 O 4 -mediated peroxymonosulphate (PMS) activation. The authors observed a decrease in Chemical Oxygen Demand (COD) of 91.01% [169].

Photocatalytic Advanced Oxidation Processes (AOP) for Heterogeneous Degradation of Antibiotics
AOP are advanced oxidative processes based on the generation of highly reactive hydroxyl (OH*) radicals. These radicals come from oxidising agents, such as hydrogen peroxide (H 2 O 2 ) or ozone (O 3 ), usually using catalysts, such as titanium dioxide (TiO 2 ) and UV light, which is known as heterogeneous catalysis [170]. Several authors reported AOP removal rates (>90%) for penicillins, sulphonamides, phenicols, β-lactams, tetracyclines, and fluoroquinolones [171][172][173][174][175]. However, the toxicity may not be totally reduced, and some intermedi-ates generated could be more toxic than the original compounds [147,176]. On the other hand, AOPs combined with biological treatments (gamma irradiation, ionising irradiation integrated with activated sludge, a biological treatment combined with flocculation and ultrafiltration) have shown high removal yields (close to 100%) of toxicants and recalcitrants [174,177].

Photodegradation of Antibiotics Using TiO 2 Based Heterogeneous Semiconductors
Photocatalysis using semiconductors is an indirect photocatalytic AOP that uses TiO 2 electrodes for organic and inorganic compound oxidation-reduction reactions. TiO 2 is non-toxic and has a high catalytic activity; however, its catalytic activity is attenuated by solar irradiation; therefore, other irradiation techniques, such as ultraviolet light (UV) have been applied [170,178].

Photo-Fenton or Electro-Fenton of Antibiotics Removal
Fenton is an advanced oxidation process in which chain reactions mediated by a non-toxic catalyst (Fe 2+ ), an acidic medium (pH = 2.8-3.0), and an oxidant (H 2 O 2 ) take place for the formation of hydroxyl radicals (OH). Several studies address the degradation of Tetracyclines (TE), Norfloxacin (NX), and Penicillin (P) using this method, showing degradation rates >80% [170,190]. Likewise, the large-scale practical application of the Fenton process has disadvantages due to the large amount of ferric sludge it generates. This solid waste sludge is considered a potential hazard due to the adsorbed organic residues of the treated wastewaters. Therefore, special treatment is required, and solid waste disposal must be at specific locations [158].

Degradation of Antibiotics in Water by Plasma Treatment
Another technique used for antibiotic removal is the dielectric barrier plasma discharge (DBD); this technique generates oxidising species: radicals (H, O, OH) and molecules, such as H 2 O 2 and O 3 for the removal of pollutants in the air and water [190]. Plasma treatment has a strong oxidising capacity, high efficiency, and experimental ease and does not require exogenous reagents. Magureanu et al. (2011) evaluated the degradation of Amoxicillin (AMC, 100%), Oxacillin (OX, 100%), and Ampicillin (AM, 100%). Amoxicillin (AMC) was degraded after 10 min of dielectric barrier discharge plasma, while the other two antibiotics degradation took around 20 to 30 min [191]. Kim et al. (2013) showed the degradation of Lincomycin (LIN), Ciprofloxacin (CIP), Enrofloxacin (ENR), Chlortetracycline (CTC), Oxytetracycline (OXT), Sulfathiazole (STZ), Sulfamethoxazole (SMX), and Trimethoprim (SXT). The results indicated that in degradation by dielectric barrier discharge plasma, the degradation rates were >90% but depending on the amount of energy supplied, and each antibiotic showed a different degradability [192]. Li et al. (2020) also assayed Tetracycline (TC), Sulfadiazine (SD), and ciprofloxacin (CIP) degradation by non-thermal discharge plasma. All antibiotics showed different degradation rates, and 20 min after plasma treatment at 19 Kv, found degradation efficiencies of 93.3, 81.2, and 58.5% for TC, SD, and CIP, respectively. The clearance efficiency and mineralisation yield of Tetracycline (TE) were relatively higher than the other antibiotics [190]. Sarangapani et al. (2019) evaluated the degradation of Ofloxacin (OFX) and Ciprofloxacin (CFX) by the atmospheric cold plasma method. Such plasmas generate the chemical reactions responsible for the formation of reactive species. The degradation was 92% and 89% for OFX and CFX, respectively, reducing the activity of both antibiotics [193].

Cathodic Degradation of Antibiotics
Cathodic degradation is an emerging technology that has potential in wastewaters treatment for nitro compounds, aromatics, azo dyes and halogenated compounds removal. This technique provides electrons for reductive degradation of pollutants, is environmentally friendly as no chemicals are needed, requires low voltage application and is low-cost [194]. Kong et al. (2015) assayed the degradation of Nitrofurazone (NFZ, 98.71%), Metronidazole (MNZ, 98.21%), Chloramphenicol (CHL, 99.86%) and Florfenicol (FLO, 99.72%) by using a dual-chamber electrochemical reactor. The different cathodic potentials applied generated a high efficiency in the degradation of the antibiotics. Finally, the authors conclude that electrochemical reduction is promising as a pretreatment or advanced treatment of wastewaters containing antibiotics [194].

Sonocatalytic Degradation of Antibiotics
Sonocatalysis is a recently advanced oxidation process (AOPs) used for water treatment using ultrasound; this process induces acoustic cavitation "in situ" to remove contaminants in a liquid phase [196,197].
Cavitation is the formation, growth and implosive collapse of gas-or vapour-filled microbubbles (considered microreactors) resulting from the acoustic wave/rarefactioninduced compression in a liquid [197]. Due to the high temperatures (≥5000 K) and pressures (≥1000 atm) reached during the process, an oxidative environment is generated with highly reactive species (ROS), such as hydrogen free radicals H*, hydroxyl (*OH) and hydroperoxyl (HO 2 *) [196,197]; species capable of degrading recalcitrant contaminants, attacking organic molecules instantaneously [196].
De Bel et al. (2009) used sonolysis at 520 kHz and 92 WL −1 for the degradation of Ciprofloxacin (CIP), finding that the degradation rate is pH-dependent due to the degree of protonation and positive charges acquired by Ciprofloxacin (CIP) during treatment which favour ultrasonic degradation. In this study, the degradation rate increased, since the pseudo-first-order degradation constant increased almost four times when comparing the treatment at pH 3 (0.021 min −1 ), pH 7 (0.0058 min −1 ) and pH 10 (0.0069 min −1 ), obtaining a BOD/COD ratio of 0.06 to 0.60; 0.17 and 0.18, after 120 min of irradiation, respectively [198].
However, the results reviewed by  also showed that: (i) the antibiotic's distance from cavitation bubbles is crucial, (ii) that removal efficiency (RE) is pH-dependent in some cases, (iii) that the hydrophobicity of the antibiotic is critical for some processes, (iv) that in some cases mineralization is not achieved, and (v) that molar volume is critical for reactions [199]; indicating that even some adjustments are necessary for the treatment of each molecule; however, the residuals to be treated are a mixture of these target molecules, among others.
In summary, the biotic, physical and chemical antibiotic degradation processes have obtained high elimination percentages (72.5-90%); however, only a few authors have reported the intermediates and possible degradation steps or sequence, because intermediates compounds generated vary according to the antibiotic degraded, the process (biological, chemical, physical, or combined) and the conditions used (pH, temperature or presence of chemicals) [200]. The identification of intermediate compounds resulting from antibiotic degradation, their toxicity, and residual antimicrobial activity is crucial to establishing the process efficiency; likewise, knowing the different degradation steps is the key to improving each treatment [201]. In this respect, Fan et al. (2019) proposed a degradation route of Cefazolin (CZ) from an advanced oxidation process with activated peroxymonosulfate (PMS); high molecular weight sulfoxide compounds were identified (Figure 1a) [202]. Some authors also have identified intermediates generated in the degradation of Cefazolin (CZ) by using oxidation reactions;  used permanganate (Mn (VII)) as an oxidant and identified five intermediates, of which one of these matches the compounds detected by Fan et al. (2019), (Figure 1b) [200]. Ghasemi et al. (2020) determined the intermediate compounds generated from the degradation of Cefazolin (CZ) from an electro Fenton (EF) process, identifying low molecular weight compounds, so the authors claim that their mineralisation using a biological treatment is possible (Figure 1c) [203].
On the other hand, the possible Cefazolin (CZ) degradation after photocatalysis processes have proposed; Gurkan et al. (2012) used UV light and TiO 2 , and the authors determined that the degradation of Cefazolin (CZ) occurs through intramolecular cleavage of lactam rings, thiadiazole, tetrazole, and subsequent reactions with -OH* radicals that transform the fragments into smaller species [204]. Figure 1d shows some of the intermediates generated. Chen et al. (2021) used a bismuth oxybromide (BiOBr) photocatalyst for the removal of Cefazolin (CZ) [205]; in this study, hydrolysis and oxidation reactions were identified (Figure 1e). Other techniques, such as sonocatalysis have also identified some intermediates (Figure 1f) [206].
Additionally, Levofloxacin degradation (LVX) has been extensively studied by different methods.  and He et al. (2022) identified the intermediates generated from chemical oxidation using peroxymonosulfate; these authors, despite studying the degradation of the same molecule using the same technique, have found that the conditions surrounding the reactions crucially influence the generated intermediates to be different; however, both authors reported obtaining small molecules that can be mineralized [207,208]. Figure 2a shows some intermediates found [207,208]. Other studies have reported the degradation of Levofloxacin (LVX) by chemical oxidation using permanganate (Mn (VII)) ( Figure 2b) [209,210]. Additionally, other authors evaluated the degradation of Levofloxacin (LVX) using Fenton and proposed the possible degradation steps (Figure 2c) [211][212][213][214].
(e) Intermediates generated from sonocatalysis [217]. There is no high evidence of degradation of antibiotics by exposure to UV light. [224] Physico-chemical

Plasma treatment
Plasma reactor in coaxial configuration operated in pulsed mode.
Oxacillin and Amoxicillin (AMC) had >90% conversion and Ampicillin (AM) was 29%. [191] Photocatalysis Solarbox 1500 photoreactor produced by an air-cooled xenon lamp. The removal of antibiotics by photocatalytic oxidation was between 40 and 100% with the exception of Clindamycin (CM) which was not removed. [225] Quartz reactor with a 20 W lamp and irradiation of 2300 µW/cm 2 .

Enzymatic Degradation of Antibiotics
For several years, the biodegradation of antibiotics by microorganisms, such as bacteria and fungi has been carried out. For example, aerobic heterotrophic bacteria, such as Actinobacteria or Proteobacteria can transform sulphonamides (to N 4 -acetylsulfamethoxazole) or even mineralise them (CO 2 and H 2 O). However, in some antibiotics, only minor changes of the molecule occur without changing the activity, while for others, intermediaries are transformed back to the parent form; as occurs in the transformation of sulphonamides to N 4 -acetylsulfamethoxazole, which can be reversible [228]. In addition, the ability to degrade antibiotics is limited in some bacteria by environmental conditions [228].
Some techniques use enzymatic degradation under controlled conditions for wastewater treatment, such as the activated sludge bioreactor (ASB), in which pollutants are submitted to aeration to promote the growth of bacteria that degrade toxic organic substances [229]. The ASB is the most widely adopted system for biological wastewater treatment; however, this method is not for antibiotics removal; for which the concentration of antibiotics removed can vary between classes [230].
On the other hand, the use of membrane bioreactors (MBR) is one of the alternatives for the removal of antibiotics, such as Ciprofloxacin (CIP), Erythromycin (E), Tetracycline (TE), Ofloxacin (OFL) and Chlortetracycline (CTC) with removal rates higher than 90% [231]. In MBR, the mechanism for antibiotics removal is enzymatic biodegradation, which is a promising alternative to eliminating these contaminants due to its low energy requirements and high efficiency [232].
For example, Wen et al. (2019) evaluated the degradation of Oxytetracycline (OXT) by immobilising LiP enzymes from Phanerochaete chrysosporium on bentonite-derived mesoporous materials; the degradation was 95% [236]. Several authors have identified the intermediates generated in biotransformation, their toxicity and possible degradation routes. Tian et al. (2020) evaluated the degradation of Oxytetracycline (OXT) using laccase produced by the fungus Pycnoporus sp., in the presence of ABTS and Al 3+ , Cu 2+ , and Fe 3+ ions. Authors showed that OXT was 100% degraded, and the antimicrobial activity reduced after treatment. In this work, the degradation pathway includes the reactions of deamination, demethylation, and dehydration. Furthermore, seven intermediates in the degradation of OXT were identified [237].
Copete The authors also demonstrated the lost antimicrobial activity of the three antibiotics and that intermediaries were shown to be non-toxic by the cytotoxicity assay [238]. Zhang et al. (2020) evaluated the degradation of antibiotics, such as Ampicillin (AM) and Tetracycline (TE) by immobilising the laccases produced by the bacterium Bacillus subtilis in a copper-trimic acid (Cu-BTC) framework. Without chemical mediators in the enzymatic treatment, a high degradation efficiency for both antibiotics (close to 100%) resulted. Eleven and fourteen intermediates, respectively, from Tetracycline (TE) and Ampicillin (AM) degradation, and the possible degradation routes, were identified. In addition, loss of antimicrobial activity and low ecotoxicity of the degradation products generated was demonstrated [67]. Sun et al. (2021) evaluated the degradation of tetracycline (TE) using the MnP enzyme. Eighty percent of Tetracycline (TE) was degraded within three hours, showing the antimicrobial activity of the transformation products from Tetracycline (TE) decreased over the reaction time. Seven compounds resulted from TE degradation and proposed a possible degradation route (demethylation, dimethylamino oxidation, decarbonylation, hydroxylation, and oxidative dehydrogenation) Sun [239].
In contrast, other authors have demonstrated the formation of toxic products due to the presence of chemical mediators. The effect of a mediator on oxidation depends on the laccase type, the substrate, the radicals formed, the mediator recyclability, and the laccase stability [4]. Weng et al. (2012) reported the degradation of Sulphadimethoxin (SDM) and Sulphamonomethoxin (SMM) using violuric acid (VLA), syringaldehyde (SIR), and 4-hydroxy benzyl alcohol (HBA) as mediators of enzymatic activity. Degradation of antibiotics using laccase in the presence of the mediators VLA or HBA generated degradation products of lower toxicity, in contrast to the treatments where the mediators were ABTS or SIR, which showed high toxicity [240]. Becker et al. (2016) reported that the mediator ABTS increased the degradation efficiencies of Sulfamethoxazole (SMX), Ampicillin (AM), and Trimethoprim (SXT), but decreased the degradation efficiency of Tetracycline (TE) and Oxytetracycline (OXT), furthermore, laccase was not able to transform Chloramphenicol (C) in the presence or absence of ABTS. The laccase combined with ABTS treatment, induced non-specific toxicity in some bioassays, involving the generation of transformation products or toxic radicals [4].
In some studies of antibiotic biotransformation, the degradation pathways, the intermediates formed, and the microorganisms and enzymes involved at each stage are still unclear. There are antibiotics widely used in humans and animals; however, their enzymatic degradation has not been assayed [231].
Chemical techniques for the removal of contaminating compounds have shown high efficiency in the degradation of antibiotics; however, they have high costs. In most of them, pollutant mineralisation is flawed, while more toxic degradation intermediates remain accumulated. For this reason, several authors have proposed that combined degradation and biodegradation processes may be the best solution for wastewater treatment. The enzymatic treatment can be pre-or post-chemical-treatment in conventional wastewaters treatment plants (WWTPs) to increase biodegradability or degrade recalcitrant compounds that were not completely removed or mineralised at the conventional ones [231]. A higher removal rate of about 20 to 50% of hybrid processes appears to be more effective for recalcitrant antibiotics removal [229].

Laccases
Laccases (Lac, E.C. 1.10.3.2) are enzymes belonging to the cupredoxin superfamily, specifically the multi-copper blue oxidase family [241]. They are ubiquitous, found in fungi, bacteria, plants, and insects, organisms in which they play different roles. In fungi, laccases allow the depolymerisation of complex compounds, such as lignin [242] morphogenesis, pigment production, and defence against stress [243]. Different genera producing laccases have been studied, such as white-rot fungi [244]. They are the most efficient organisms in lignin degradation until their mineralisation to CO 2 and water. Lignin is the second most abundant biopolymer after cellulose and is one of the most recalcitrant natural compounds [245]. Lignin degradation, like that of other xenobiotic compounds, occurs due to the production of low specificity extracellular ligninolytic oxidoreductase enzymes, classified as phenol oxidases and haem-peroxidases [246]. Phenol-oxidase enzymes use oxygen (O 2 ) as the final electron acceptor, whereas haem-peroxidases use hydrogen peroxide (H 2 O 2 ) as the final electron acceptor. Phenol-oxidase enzymes include laccases, while haem-peroxidases include enzymes, such as lignin peroxidase (LiP, E.C 1.11.1.14), manganese peroxidase (MnP, E.C 1.11.1.13), versatile peroxidase (VP, E.C 1.11.1.16) and dyP-type peroxidases (E.C. 1.11.1.19) [242,247,248]. Additionally, there are accessory enzymes involved in lignin degradation, which produce hydrogen peroxide (H 2 O 2 ) required by peroxidases, such as aryl alcohol oxidase (E.C. 1.1.3.7), glyoxal oxidase (E.C. 1.2.3.5) and glucose 1-oxidase (E.C. 1.1.3.4) [246]. The activity and ability of these enzymes to degrade compounds vary according to species and catalytic properties [248]. Laccase enzymes are the main enzymes involved in lignin degradation, and the rate of lignin degradation is related to the production of laccases. Currently, laccases are of great industrial importance as they can catalyse the oxidation of a large number of compounds and are also of great importance in the treatment of pesticides, explosives, wastewaters and synthetic dyes [243], and wastes generated by different industries, mainly paper, petrochemical and textile industries [249]. These waste compounds harm the environment; for example, synthetic dyes have complex chemical structures that are difficult to degrade and harm the environment and health, as they are toxic, carcinogenic and highly recalcitrant [250][251][252].

Laccase and the Degradation of Antibiotics Laboratory Studies
Tetracycline's broad action spectrum and low-cost favours its wide use in humans and animals. These antibiotics are frequently in wastewaters, and their degradation contributes to a significant decrease in antibiotic contamination. Therefore, Tetracycline (TE) degradation by using laccases has previously been reported. Zhang et al. (2020) obtained a degradation percentage of tetracycline (1 mg mL −1 ) close to 100%, using immobilised laccases from Bacillus subtilis [67]. Moreover, some drugs are cytotoxic, mutagenic, teratogenic and carcinogenic, even at low concentrations; doxorubicin has been detected in hospital wastewaters, as it is excreted in urine from 3 to 10% and in faeces from 40 to 50% of the dose administered to the patient. Doxorubicin at concentrations of 0.05 µg L −1 causes DNA damage in Ceriodaphnia dubia. Kelbert et al. (2020) evaluated the degradation of doxorubicin using laccases from Trametes versicolor. The laccases degraded different concentrations (50,250, and 500 µg L −1 ) of doxorubicin in 4 h; the assay conditions were 1800 UL −1 of laccases, pH 7.0 ± 0.2, and 30 • C [256].

Computational Studies for Antimicrobial Degradation Using Laccases
Bioinformatics tools facilitate the prediction of molecular mechanisms and the behaviour of enzymes against different ligands to obtain a prior estimation of their industrial potential use in bioremediation processes. Moreover, it is economically convenient to perform this computational simulation before experimental trials. The interaction enzymesubstrate can be studied through molecular docking [257][258][259][260][261][262][263] and molecular dynamics analyses [264]; for these studies, algorithms allow for calculating the electrostatic interactions of the amino acid residues in contact with the specific substrates (ligands) to be studied [265].
Computer tools allow the prediction of the properties of a molecule by homology modelling of its 3D structure. Structural knowledge of molecules is a prerequisite for molecular docking analyses, but this is the major limitation because, in most cases, the 3D structure of proteins is unknown, as they have not been purified and crystallised [257,[259][260][261][262][263][264]266]; making it necessary to work with simulated models.
Experimental procedures for structure determination have certain disadvantages and limitations because, during the crystallisation of highly hydrated crystals, they can distort some regions as it is sometimes unclear whether an atom corresponds to the protein or the water molecule. Likewise, using techniques, such as Nuclear Magnetic Resonance (NMR) can determine the structure of proteins; however, these must be small proteins (less than 30 kDa), and difficult to crystallise [260]. Experimental structure determination is also expensive and time-consuming [267].
There are computational studies on interactions between laccases and different pollutants from the pharmaceutical industry. Sakar et al. (2020) analysed the computational structure of laccases produced by Thermus thermophilus; the enzymes were characterised under physicochemical parameters, determining that they are mainly composed of negatively charged aliphatic amino acids, mostly valine and to a lesser extent cysteine. Additionally, due to the high value of the aliphatic index (41.29) and the presence of salt bridges in their structure, they are thermostable enzymes. In addition, identified random folding regions played a role in the flexibility and conformational changes of the protein. On the other hand, the Ramachandran analysis shows the statistical distribution of the torsion angles of the amino acid residues of a protein in the sterically favoured, allowed and not allowed regions; in this case, more than 90% of the amino acids were located in protein regions that were energetically favoured; demonstrating a high quality of the model. In addition, according to the negative value of hydropathy, they confirmed the hydrophilic nature of laccases and identified the binding sites and functional motifs [267].
Cárdenas-Moreno et al. (2019) computationally evaluated the interactions of Ganoderma weberianum laccases with different pharmaceutical compounds, including Tetracycline (TE) and Sulfixazole (SXZ). The authors modelled and optimised the laccase 3D structure, identifying the active substrate binding sites, and the allowed angular conformations to establish the docking coordinates and modelled the ligands. The authors ran analyses taking into account random conformations and the number of interactions, using ABTS (−7.1 kcal mol −1 ) and DMP (−4.8 kcal mol −1 ) as controls. Tetracycline (TE) has no interaction with any binding site; however, it had an energy of −5.2 kcal mol −1 , probably due to the high density of polar groups unable to interact with the amino acid residues of the enzyme active site. Sulfisoxazole (SXZ) had an energy of −6.8 kcal mol −1 ; a hydrophobic interaction with the His 423 , which is in the H-X-H motif at the CuIII copper-binding site, and a minor atomic interaction distance of 7 Å, was detected. This distance between a ligand atom and a receptor atom indicates the relative orientation of the ligands. Small distances (<1.5 Å) represent an atomic overlap generating a major repulsive force, while large distances result in a less effective energy constant [269].
On the other hand, the authors calculated the root mean square deviation (RMSD), in this type of analysis, the structural comparison results from the alignment with crystallised structures. The lower the RMSD is, the higher the similarity between the compared molecules. However, this criterion depends on molecule size [260,270] because values lower than 3.0 Å (typical RMSD values for homologous proteins) indicate high-quality models. In this case, an RMSD = 1.991 was obtained for laccase-Sulfixazole and RMSD = 1.318 for laccase-tetracycline interaction, supporting the ability of the active laccase binding site to interact with these ligands [271].

Ultrasound (Sonolysis) Combined with Enzymatic Degradation for the Degradation of Antibiotics
Sutar and Rathod (2015) worked out the catalytic degradation of Ciprofloxacin (CIP) but using Laccase (Lac, E.C. 1.10.3. 2) in a combined process with sonolysis, achieving 51% degradation under specific conditions (0.02% (w/v) enzyme loading; 60 • C; 75 W input power; 22 kHz frequency; 50% duty cycle and 200 rpm agitation); exceeding the degradation obtained in the conventional process (16%) and reducing the treatment time [272]. Chakma et al. (2020) investigated the links between enzymatic (Horseradish Peroxidase (HRP, EC: 1.11.1.7) coupled with ultrasound processes for Ciprofloxacin (CIP) degradation and found that ultrasound and cavitation improve the degradation efficiency by increasing the interaction between enzyme and organic molecules (kinetic energy). In this sense, Ciprofloxacin (CIP) is enzymatically transformed, so there is no significant improvement in the sono-enzymatic process at which the highest degradation rates at optimum conditions (pH 7.0 and 25 • C) were 68.41%. In contrast, the intermediates, such as acetic acid, ethyl alcohol and other products with -OH groups generated during the sono-enzymatic reaction are less toxic than those formed in the enzymatic process; this means that ultrasound favours mineralization by degrading the intermediates formed during enzymatic treatment [196].

Conclusions
Antibiotics should be utilised by veterinarians-or physicians-prescription either for metaphylaxis or prophylaxis; however, the indiscriminate use as a growth factor in animals (a prohibited activity in several countries) and by self-medication in humans generates a great effect on the environment and favours the increase and dissemination of antibioticresistant microorganisms. As this review shows, the chemical nature of many antibiotics facilitates their excretion through urine or faeces, both in animals and humans. The use of antibiotics is indispensable, despite many international organisations speaking out in favour of their controlled use, many countries are not complying with the rules and continue with inadequate practices, such as the use of antibiotics exclusively for humans in animals, their use as growth factors, biocides in crops, the lack of adequate treatment of wastewaters in pharmaceutical plants, senior residences, farms and hospitals, the use of animal manure as part of biofertilisers and the use of antibiotics as a supplement in animal nutrition, among others.
It is clear that the majority of the antibiotics are recalcitrant and toxic to the environment as they can trigger eutrophication leading to water pollution and nutrient imbalance, and the exposition of microorganisms to the sublethal concentrations of antibiotics also press antibiotic resistance, leading irremediably to the transmission of resistant microorganisms and consequently therapeutic failure in humans and animals.
Many physical and chemical procedures have shown to be effective and improve the management of wastewaters containing antibiotics residues, but the treatment of these wastewaters using enzymes (including laccases) is very promising and appears to be less costly.