Development of a Novel Class of Pyridazinone Derivatives as Selective MAO-B Inhibitors

Sixteen compounds (TR1–TR16) were synthesized and evaluated for their inhibitory activities against monoamine oxidase A and B (MAOs). Most of the derivatives showed potent and highly selective MAO-B inhibition. Compound TR16 was the most potent inhibitor against MAO-B with an IC50 value of 0.17 μM, followed by TR2 (IC50 = 0.27 μM). TR2 and TR16 selectivity index (SI) values for MAO-B versus MAO-A were 84.96 and higher than 235.29, respectively. Compared to the basic structures, the para-chloro substituent in TR2 and TR16 increased the inhibitory activity of MAO-B. TR2 and TR16 were reversible MAO-B inhibitors that were competitive, with Ki values of 0.230 ± 0.004 and 0.149 ± 0.016 µM, respectively. The PAMPA method indicated that compounds TR2 and TR16 had the tendency to traverse the blood–brain barrier. Docking investigations revealed that lead compounds were beneficial for MAO-B inhibition via association with key as well as selective E84 or Y326 residues, but not for MAO-A inhibition via interaction primarily driven by hydrophobic contacts. In conclusion, TR2 and TR16 are therapeutic prospects for the management of multiple neurodegenerative diseases.


Introduction
Parkinson's disease (PD) is a neurological illness that affects 6.1 million individuals throughout the world [1]. Parkinsonism is a syndrome characterized by rigidity, bradykinesia, rest tremor, and postural instability [1][2][3]. PD is defined pathologically by the depletion of dopamine neurons in nigra pars compacta, which is followed by Lewy bodies, which are cytoplasmic inclusions within the midbrain comprising insoluble alpha-synuclein clusters. Nevertheless, PD is distinguished by more common pathology in different areas of the brain, which encompasses nondopaminergic neurons. [2]. The symptoms of disease can be treated with drugs that increase the level of dopamine, though they are not so effective.
Monoamine oxidases (MAO-A and MAO-B) are key enzymes that deaminate biogenic amines in the tissues of both peripheral and brain regions, controlling neurotransmitter levels including dopamine, norepinephrine, and epinephrine [4,5]. Selective MAO-A inhibitors have been shown to be useful in the management of depression [6]. Selective MAO-B inhibitors limit dopamine degradation in the central nervous system (CNS), blocking dopamine reduction. MAO-B inhibitors not only impede dopamine degradation, but also diminish the generation of neurotoxic byproducts of the MAO enzymatic reaction, such as hydrogen peroxide and aldehyde. L-Dopa has always remained the cornerstone of Parkinson's therapy and continues to be the most efficacious suggestive therapeutic medication. L-Dopa is often used with selective MAO-B inhibitors. As a corollary, adopting MAO-B inhibitors in the initial phases of the disease may postpone the emergence of significant symptoms and the necessity for L-dopa. [7].
In our previous reports, the synthesis of 3(2H)-pyridazinone candidates and the exploration of their MAO inhibitory properties were described. In the reports, new classes of pyridazinone core with substitution of benzalhydrazones, piperazine and morpholine analogues were designed and synthesized [17]. In addition, it was hypothesized that the addition of substituted benzalhydrazone to the second position of pyridazinone and the substitution of electron withdrawing groups in the benzalhydrazone ring caused MAO-B inhibitory activity to increase [18].
In particular, in this study, we aimed to synthesize, characterize, and determine the MAO-B inhibition activity of pyridazinone derivatives according to the hypothesis as shown below (Scheme 1).

Medazomide Antitussif
In our previous reports, the synthesis of 3(2H)-pyridazinone candidates and the ploration of their MAO inhibitory properties were described. In the reports, new cla of pyridazinone core with substitution of benzalhydrazones, piperazine and morpho analogues were designed and synthesized [17]. In addition, it was hypothesized that addition of substituted benzalhydrazone to the second position of pyridazinone and substitution of electron withdrawing groups in the benzalhydrazone ring caused MA inhibitory activity to increase [18].
In particular, in this study, we aimed to synthesize, characterize, and determine MAO-B inhibition activity of pyridazinone derivatives according to the hypothesi shown below (Scheme 1). Scheme 1. Rational design of the new hMAO inhibitors.

General Chemistry of Synthetic Scheme
The targeted pyridazinone compounds (TR1-16) were synthesized in accorda with the literature, as shown in Scheme 2.
The nucleophilic substitution reaction of 3,6-dichloropyridazine with phenylpip Scheme 1. Rational design of the new hMAO inhibitors. Table 1. Some therapeutic drugs with pyridazinone core.

Structure
Compound Activity ecules with the 3(2H)-pyridazinone framework have been undertaken. Multiple bioactivities have really been documented for the compounds, including analgesic, anti-inflammatory, antihypertensive, cardiotonic, antiplatelet, anticholinesterase, anti-bacterial, antifungal, and antitumoral effects. [15,16]. In particular, pyridazinone derivatives are recognized as agents with noteworthy effects in the cardiovascular system attributed to their inhibition of platelet aggregation, antihypertensive activity, and cardiotonic qualities (Table 1).

Structure Compound Activity
Zardaverine Cardiotonic

Structure Compound Activity
Zardaverine Cardiotonic

Imazodan Cardiotonic
Levosimendan Vasodilator ecules with the 3(2H)-pyridazinone framework have been undertaken. Multiple bioactivities have really been documented for the compounds, including analgesic, anti-inflammatory, antihypertensive, cardiotonic, antiplatelet, anticholinesterase, anti-bacterial, antifungal, and antitumoral effects. [15,16]. In particular, pyridazinone derivatives are recognized as agents with noteworthy effects in the cardiovascular system attributed to their inhibition of platelet aggregation, antihypertensive activity, and cardiotonic qualities (Table 1). ities have really been documented for the compounds, including analgesic, anti-inflammatory, antihypertensive, cardiotonic, antiplatelet, anticholinesterase, anti-bacterial, antifungal, and antitumoral effects. [15,16]. In particular, pyridazinone derivatives are recognized as agents with noteworthy effects in the cardiovascular system attributed to their inhibition of platelet aggregation, antihypertensive activity, and cardiotonic qualities (Table 1). Emorfazone Analgesic

Medazomide Antitussif
In our previous reports, the synthesis of 3(2H)-pyridazinone candidates and the exploration of their MAO inhibitory properties were described. In the reports, new classes of pyridazinone core with substitution of benzalhydrazones, piperazine and morpholine analogues were designed and synthesized [17]. In addition, it was hypothesized that the addition of substituted benzalhydrazone to the second position of pyridazinone and the substitution of electron withdrawing groups in the benzalhydrazone ring caused MAO-B inhibitory activity to increase [18].
In particular, in this study, we aimed to synthesize, characterize, and determine the MAO-B inhibition activity of pyridazinone derivatives according to the hypothesis as shown below (Scheme 1). Emorfazone Analgesic

Medazomide Antitussif
In our previous reports, the synthesis of 3(2H)-pyridazinone candidates and the exploration of their MAO inhibitory properties were described. In the reports, new classes of pyridazinone core with substitution of benzalhydrazones, piperazine and morpholine analogues were designed and synthesized [17]. In addition, it was hypothesized that the addition of substituted benzalhydrazone to the second position of pyridazinone and the substitution of electron withdrawing groups in the benzalhydrazone ring caused MAO-B inhibitory activity to increase [18].
In particular, in this study, we aimed to synthesize, characterize, and determine the MAO-B inhibition activity of pyridazinone derivatives according to the hypothesis as shown below (Scheme 1).

General Chemistry of Synthetic Scheme
The targeted pyridazinone compounds (TR1-16) were synthesized in accordance with the literature, as shown in Scheme 2.
The nucleophilic substitution reaction of 3,6-dichloropyridazine with phenylpiperazine or morpholine derivatives in ethanol was carried out to produce 3-chloro-6-substituted pyridazine. The hydrolysis of 3-chloro-6-(4-(3,4-dichlorophenyl)piperazine-1-yl)pyridazine by heating in glacial acetic acid afforded 6-(4-(3-Methoxy/3-trifluoromethylphenyl)piperazi ne-1-yl/morpholino)-3(2H)-pyridazinone [19]. Subsequently, the reaction of 6-(4-(3-methoxy /3-tifluoromethylphenyl)piperazine-1-yl/morpholino)-3(2H)-pyridazinone with ethyl bromoacetate in the presence of K 2 CO 3 in acetone replaced the hydrogen atom of tertiary nitrogen atom in the pyridazinone ring and formed ethyl 6-(4-(3-methoxy/3-trifluorome thylphenyl)piperazine-1-yl/morpholino)-3(2H)-pyridazinone-2-ylacetate. The introduction of hydrazine hydrate (99%) can remove the ethyl group from the former intermediate and generate corresponding acid hydrazides [20]. Finally, nucleophilic addition reaction with various substituted aromatic aldehydes with acid hydrazides resulted in the formation of final targeted candidates (TR1-TR16). In this study, all of the title compounds were published for the first time. The compounds' reaction yields vary from around 57% to 92%. The maximum yield (91.67%) was achieved with compound TR8, while the lowest yield was achieved with compound TR3 (57.10%). All structures were validated using 1 H-NMR, 13 C-NMR and mass spectral data. Table 2 lists the molecular structures and  physical characterization of TR1-TR16. olecules 2022, 27, x FOR PEER REVIEW 4 of 13 nucleophilic addition reaction with various substituted aromatic aldehydes with acid hydrazides resulted in the formation of final targeted candidates (TR1-TR16). In this study all of the title compounds were published for the first time. The compounds' reaction yields vary from around 57% to 92%. The maximum yield (91.67%) was achieved with compound TR8, while the lowest yield was achieved with compound TR3 (57.10%). All structures were validated using 1 H-NMR, 13 C-NMR and mass spectral data. Table 2 lists the molecular structures and physical characterization of TR1-TR16. Scheme 2. Synthesis of the compounds TR1-16.  nucleophilic addition reaction with various substituted aromatic aldehydes with acid hydrazides resulted in the formation of final targeted candidates (TR1-TR16). In this study, all of the title compounds were published for the first time. The compounds' reaction yields vary from around 57% to 92%. The maximum yield (91.67%) was achieved with compound TR8, while the lowest yield was achieved with compound TR3 (57.10%). All structures were validated using 1 H-NMR, 13 C-NMR and mass spectral data. Table 2 lists the molecular structures and physical characterization of TR1-TR16. Scheme 2. Synthesis of the compounds TR1-16.  Piperazine signals in compounds TR1-13 were observed approximately between 3.2 and 3.5 ppm in 1 H-NMR. Morpholine signals in compounds TR14-16 were shifted between 3.2 and 3.68 ppm due to the oxygen atom in the ring (-OCH2-). Unlike other compounds TR7-16, compounds TR1-6 with -OCH3 at the R 1 position had a singlet signal at 3.80 ppm in 1 H-NMR and a signal at 55 ppm in 13 C-NMR. Compounds TR5 and TR11 with -CH3 at the R2 position were confirmed by the signal at 2.39 ppm in 1 H-NMR and the signal at 21 ppm in 13 C-NMR. TR12 with 4-N(CH3)2 at the R2 position was confirmed by the signal at 3.04 ppm (6H, integral) in 1 H-NMR.

MAO Inhibition Studies
At 10 μM, all compounds evaluated exhibited higher than 50% residual activity for MAO-A; however, only six compounds showed lower than 50% residual activity for MAO-B (Table 3). TR16 had the strongest inhibitory action against MAO-B, with an IC50 value of 0.17 μM, followed by TR2 (IC50 = 0.27 μM) ( Table 3). The substituent -Cl atom at the para position of TR16 showed 235.3-and 2.5-times higher MAO-B inhibitory activity over the basic structure TR14 (IC50 = >40 μM) and -F atom at the para position of TR15 (IC50 = 0.43 μM), respectively. On the other hand, the -Cl atom at the para position of TR2 increased MAO-B inhibitory potency 148.1-fold (IC50 = 0.27 μM) compared to the basic structure TR1 (IC50 = >40 μM). When TR2 and TR16 were compared, TR16 had a 1.59-fold higher IC50 for MAO-B than TR2. Both TR2 and TR16 showed selective inhibition for MAO-B with selectivity index (SI) values of >235.29 and 84.96, respectively ( Table 3). The -OCH3 substituted derivatives at the R1 position (TR1-6) showed higher MAO-B inhibitory activity than the -CF3 substituted derivatives at the same position (TR7-13), except for the derivatives with -F substituent at the para position of R2, i.e., TR3 and TR9. However, substitutions of -OCH3 and -CF3 at the R1 position did not show a significant difference in MAO-A inhibitory activity. Piperazine signals in compounds TR1-13 were observed approximately between 3.2 and 3.5 ppm in 1 H-NMR. Morpholine signals in compounds TR14-16 were shifted between 3.2 and 3.68 ppm due to the oxygen atom in the ring (-OCH 2 -). Unlike other compounds TR7-16, compounds TR1-6 with -OCH 3 at the R 1 position had a singlet signal at 3.80 ppm in 1 H-NMR and a signal at 55 ppm in 13 C-NMR. Compounds TR5 and TR11 with -CH 3 at the R 2 position were confirmed by the signal at 2.39 ppm in 1 H-NMR and the signal at 21 ppm in 13 C-NMR. TR12 with 4-N(CH 3 ) 2 at the R 2 position was confirmed by the signal at 3.04 ppm (6H, integral) in 1 H-NMR.

MAO Inhibition Studies
At 10 µM, all compounds evaluated exhibited higher than 50% residual activity for MAO-A; however, only six compounds showed lower than 50% residual activity for MAO-B (Table 3). TR16 had the strongest inhibitory action against MAO-B, with an IC 50 value of 0.17 µM, followed by TR2 (IC 50 = 0.27 µM) ( Table 3). The substituent -Cl atom at the para position of TR16 showed 235.3-and 2.5-times higher MAO-B inhibitory activity over the basic structure TR14 (IC 50 = >40 µM) and -F atom at the para position of TR15 (IC 50 = 0.43 µM), respectively. On the other hand, the -Cl atom at the para position of TR2 increased MAO-B inhibitory potency 148.1-fold (IC 50 = 0.27 µM) compared to the basic structure TR1 (IC 50 = >40 µM). When TR2 and TR16 were compared, TR16 had a 1.59-fold higher IC 50 for MAO-B than TR2. Both TR2 and TR16 showed selective inhibition for MAO-B with selectivity index (SI) values of >235.29 and 84.96, respectively ( Table 3). The -OCH 3 substituted derivatives at the R 1 position (TR1-6) showed higher MAO-B inhibitory activity than the -CF 3 substituted derivatives at the same position (TR7-13), except for the derivatives with -F substituent at the para position of R 2 , i.e., TR3 and TR9. However, substitutions of -OCH 3 and -CF 3 at the R 1 position did not show a significant difference in MAO-A inhibitory activity.

Kinetic Study
Five different substrate concentrations were used in the kinetics. TR2 and TR16 met a point on the y-axis in the Lineweaver-Burk plots ( Figure 1A,C), and their secondary plots exhibited K i values of 0.230 ± 0.004 and 0.149 ± 0.016 µM, respectively, in the kinetic investigations for MAO-B. (Figure 1B,D). TR2 and TR16 are competitive inhibitors that compete with the substrate and bind to the MAO-B active site.

Reversibility Studies
TR2 and TR16 concentrations in the reversibility studies were 0.54 and 0.34 µM, respectively, whereas the respective concentrations for the references, the reversible inhibitor lazabemide and the irreversible inhibitor pargyline, were 0.22 and 0.28 µM, respectively. The relative activity of undialyzed (AU) as well as dialyzed (AD) samples were compared to establish reversibility profiles. TR2 and TR16 inhibitions of MAO-B were restored from 28.0 % (AU) to 75.0 % (AD), and from 31.8% to 73.4%, respectively ( Figure 2). When compared to the reference inhibitor, the compounds recovered to the levels comparable to lazabemide, the reference reversible inhibitor against MAO-B (varying between 25.0% and 73.4%), but were distinct from pargyline, a reference irreversible inhibitor against MAO-B. (i.e., varying between 34.5% and 35.2%). These data revealed that TR2 and TR16 were reversible inhibitors of MAO-B.
Five different substrate concentrations were used in the kinetics. TR2 and TR16 met a point on the y-axis in the Lineweaver-Burk plots ( Figure 1A,C), and their secondary plots exhibited Ki values of 0.230 ± 0.004 and 0.149 ± 0.016 μM, respectively, in the kinetic investigations for MAO-B. (Figure 1B,D). TR2 and TR16 are competitive inhibitors that compete with the substrate and bind to the MAO-B active site.

Reversibility Studies
TR2 and TR16 concentrations in the reversibility studies were 0.54 and 0.34 μM, respectively, whereas the respective concentrations for the references, the reversible inhibitor lazabemide and the irreversible inhibitor pargyline, were 0.22 and 0.28 μM, respectively. The relative activity of undialyzed (AU) as well as dialyzed (AD) samples were compared to establish reversibility profiles. TR2 and TR16 inhibitions of MAO-B were restored from 28.0 % (AU) to 75.0 % (AD), and from 31.8% to 73.4%, respectively ( Figure  2). When compared to the reference inhibitor, the compounds recovered to the levels comparable to lazabemide, the reference reversible inhibitor against MAO-B (varying between 25.0% and 73.4%), but were distinct from pargyline, a reference irreversible inhibitor against MAO-B. (i.e., varying between 34.5% and 35.2%). These data revealed that TR2 and TR16 were reversible inhibitors of MAO-B.

Reversibility Studies
TR2 and TR16 concentrations in the reversibility studies were 0.54 and 0.34 μM, respectively, whereas the respective concentrations for the references, the reversible inhibitor lazabemide and the irreversible inhibitor pargyline, were 0.22 and 0.28 μM, respectively. The relative activity of undialyzed (AU) as well as dialyzed (AD) samples were compared to establish reversibility profiles. TR2 and TR16 inhibitions of MAO-B were restored from 28.0 % (AU) to 75.0 % (AD), and from 31.8% to 73.4%, respectively ( Figure  2). When compared to the reference inhibitor, the compounds recovered to the levels comparable to lazabemide, the reference reversible inhibitor against MAO-B (varying between 25.0% and 73.4%), but were distinct from pargyline, a reference irreversible inhibitor against MAO-B. (i.e., varying between 34.5% and 35.2%). These data revealed that TR2 and TR16 were reversible inhibitors of MAO-B.

Parallel Artificial Membrane Permeability Assay (PAMPA)
The blood-brain barrier (BBB) permeation of the lead pyridaziones molecules (TR2 and TR16) was confirmed by PAMPA [21]. The compounds showed good CNS permeabilities, with CNS+ positive standards such as progesterone and verapamil (Table 4).

Parallel Artificial Membrane Permeability Assay (PAMPA)
The blood-brain barrier (BBB) permeation of the lead pyridaziones molecules (TR2 and TR16) was confirmed by PAMPA [21]. The compounds showed good CNS permeabilities, with CNS+ positive standards such as progesterone and verapamil (Table 4).     Compounds TR2, TR15, and TR16 interact within the MAO-A binding site through hydrophobic contacts. Morpholine ring of TR15 and TR16 faces the aromatic rings of FAD, Y407, and Y444, a binding room not engaged by the meta-methoxy ring of TR2, due to its bulky structure.
As shown in Figure 4, compound TR2 reaches the aromatic cage formed by FAD, Y398, and Y435 through its meta-methoxy ring and establishes a hydrogen bond with the side chain of E84, a MAO-B selective residue (changed to V93 in MAO-A). Moreover, compounds TR15 and TR16 engage the same interactions with Y398 through π-π contacts and with the phenol group of MAO-B selective residue Y326 by forming a hydrogen bond with the carbonyl groups of the pyridazinone rings. Overall, molecular docking analyses suggested that compounds were not very effective at engaging the MAO-A binding pocket, due to the interaction mainly driven by hydrophobic contacts. Finally, the induced-fit docking protocol was used in order to deepen the study concerning the interactions occurring between TR16 and MAO-B ( Figure 5).   were reported as green, cyan, and magenta sticks, respectively. Red arrows and green lines indicate hydrogen bonds and π-π contacts, respectively. For completeness, docking scores returned from simulations were equal to −10.051, −9.452 and −8.558 kcal/mol for TR2, TR15 and TR16, respectively. (C) were reported as green, cyan, and magenta sticks, respectively. Red arrows and green lines indicate hydrogen bonds and π-π contacts, respectively. For completeness, docking scores returned from simulations were equal to −10.051, −9.452 and −8.558 kcal/mol for TR2, TR15 and TR16, respectively.
Molecules 2022, 27, x FOR PEER REVIEW 9 of 13 Compounds TR2, TR15, and TR16 interact within the MAO-A binding site through hydrophobic contacts. Morpholine ring of TR15 and TR16 faces the aromatic rings of FAD, Y407, and Y444, a binding room not engaged by the meta-methoxy ring of TR2, due to its bulky structure.
As shown in Figure 4, compound TR2 reaches the aromatic cage formed by FAD, Y398, and Y435 through its meta-methoxy ring and establishes a hydrogen bond with the side chain of E84, a MAO-B selective residue (changed to V93 in MAO-A). Moreover, compounds TR15 and TR16 engage the same interactions with Y398 through π-π contacts and with the phenol group of MAO-B selective residue Y326 by forming a hydrogen bond with the carbonyl groups of the pyridazinone rings. Overall, molecular docking analyses suggested that compounds were not very effective at engaging the MAO-A binding pocket, due to the interaction mainly driven by hydrophobic contacts. Finally, the induced-fit docking protocol was used in order to deepen the study concerning the interactions occurring between TR16 and MAO-B ( Figure 5). As reported in Figure 5, the binding mode of TR16 was consistent with previous standard docking simulation. In detail, the interactions with selective residue Y326 and with Y398 were detected, and an additional aromatic residue, F343, was recruited to engage a new π-π contact, with a little shift from its initial position.

Bioavailability Prediction
The bioavailability of lead molecules was predicted by SwissADME online platform. The bioavailability radar panel assesses drug candidates based on physicochemical parameters such as lipophilicity, flexibility, polarity insolubility, size, and saturation [22]. Interestingly, both TR2 and TR16 molecules had been reported to be in the pink region (optimal ranges) and to have drug-like properties ( Figure 6). As reported in Figure 5, the binding mode of TR16 was consistent with previous standard docking simulation. In detail, the interactions with selective residue Y326 and with Y398 were detected, and an additional aromatic residue, F343, was recruited to engage a new π-π contact, with a little shift from its initial position.

Bioavailability Prediction
The bioavailability of lead molecules was predicted by SwissADME online platform. The bioavailability radar panel assesses drug candidates based on physicochemical parameters such as lipophilicity, flexibility, polarity insolubility, size, and saturation [22]. Interestingly, both TR2 and TR16 molecules had been reported to be in the pink region (optimal ranges) and to have drug-like properties ( Figure 6).

Chemistry
The synthetic procedures of the titled compounds are provided in the supplementary information.

Enzyme Assays
Recombinant human MAO-A and MAO-B, AChE from Electrophorus electricus, BChE from equine serum, BACE1, and their substrates and reference inhibitors were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Enzyme Assays
Recombinant MAO-A and MAO-B were used as substrates in MAO inhibitory activity assays using kynuramine (0.06 mM) and benzylamine (0.3 mM), respectively [23]. The MAO-A and MAO-B reactions were carried out at 25 °C and monitored for 30 min at 316 and 250 nm, respectively, in 0.5 mL mixture of 50 mM sodium phosphate (pH 7.2). Clorgyline and toloxatone were the drug references used for MAO-A, and pargyline and lazabemide were used for MAO-B as reference compounds. The IC50 values were determined by measuring the residual activity at different concentrations of the compounds and by using GraphPad Prism software 5.0. The Km value of benzylamine against MAO-B was measured to be 0.40 mM [24]. On the other hand, multitarget analysis, ChE (AChE, BChE) inhibitory activity assays were carried out at 25 °C and monitored for 15 min at 412 nm in a 0.5 mL mixture of 100 mM sodium phosphate (pH 7.5). BACE1 inhibitory activities were tested by the BACE1 activity detection kit. The assay method was described previously [25].

Inhibition Profile of MAOs and Kinetic Studies
TR1 and TR16 MAO inhibitory actions were evaluated at 10 μM for primary screening. For the compounds with <80% residual activities, their IC50 values were calculated. TR2 and TR16 kinetics with MAO-B were investigated at five different substrate concentrations (0.0375-0.6 μM). Lineweaver-Burk plots were used to examine the inhibition patterns, and secondary plots were used to calculate the Ki values with three inhibitor dosages [26][27][28].

Chemistry
The synthetic procedures of the titled compounds are provided in the supplementary information.

Enzyme Assays
Recombinant MAO-A and MAO-B were used in MAO inhibitory activity assays using kynuramine (0.06 mM) and benzylamine (0.3 mM) as substrates, respectively [23]. The MAO-A and MAO-B reactions were carried out at 25 • C and monitored for 30 min at 316 and 250 nm, respectively, in 0.5 mL mixture of 50 mM sodium phosphate (pH 7.2). Clorgyline and toloxatone were the drug references used for MAO-A, and pargyline and lazabemide were used for MAO-B as reference compounds. The IC 50 values were determined by measuring the residual activity at different concentrations of the compounds and by using GraphPad Prism software 5.0. The K m value of benzylamine against MAO-B was measured to be 0.40 mM [24]. On the other hand, multitarget analysis, ChE (AChE, BChE) inhibitory activity assays were carried out at 25 • C and monitored for 15 min at 412 nm in a 0.5 mL mixture of 100 mM sodium phosphate (pH 7.5). BACE1 inhibitory activities were tested by the BACE1 activity detection kit. The assay method was described previously [25]. Recombinant human MAO-A and MAO-B, AChE from Electrophorus electricus, BChE from equine serum, BACE1, and their substrates and reference inhibitors were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Inhibition Profile of MAOs and Kinetic Studies
TR1 and TR16 MAO inhibitory actions were evaluated at 10 µM for primary screening. For the compounds with <80% residual activities, their IC 50 values were calculated. TR2 and TR16 kinetics with MAO-B were investigated at five different substrate concentrations (0.0375-0.6 µM). Lineweaver-Burk plots were used to examine the inhibition patterns, and secondary plots were used to calculate the K i values with three inhibitor dosages [26][27][28].

Inhibition Reversibility of TR2 and TR16
After preincubation with the enzyme for 30 min at~2 × IC 50 (i.e., 0.54 and 0.34 µM, respectively), the reversibility of TR2 or TR16 against MAO-B inhibition was evaluated using a dialysis approach and employing a dialysis kit, as previously described [29,30]. Lazabemide (a reversible MAO-B inhibitor) and pargyline (an irreversible MAO-B inhibitor) were preincubated at 0.22 and 0.28 µM, respectively, as reference compounds. To determine reversibility patterns, the activities of dialyzed (AD) and undialyzed (AU) specimens were compared.

BBB Study by PAMPA Method
Using the PAMPA method, blood-brain barrier permeation abilities of lead compounds TR2 and TR16 were analyzed [21].

Docking Studies
X-ray crystallographic structures of MAO-A and MAO-B were taken from the Protein Data Bank by finding records 2Z5X and 2V5Z, respectively [31,32]. With the assistance of the protein preparation wizard tool, both protein structures were evaluated for the ionization states of acid/basic side chains at physiological pH, to remove nonfunctional water and to carry out energy minimization steps [33,34]. Ligands to be docked were prepared by using the Ligprep tool to sample allowed tautomers and ionization states for all the possible conformers. Standard docking protocol (SP) was carried out using GLIDE by utilizing the OPLS3 force field and setting 50,000 poses per ligand for the first phase and 4000 poses per ligand for energy minimization [35][36][37]. Best poses yielded Root Mean Square Deviation (RMSD) values of 0.39 Å and 0.76 Å for MAO-A and MAO-B cognate ligands, respectively, which was satisfactory. Eventually, the induced-fit docking protocol was employed in order to better understand the interactions between compound TR16 and MAO-B. This protocol allows the conformational changes in residues involved in the ligand binding to be considered, thus enhancing the reliability and the accuracy of computational simulations [38]

Conclusions
The current work evaluated the MAO-A and MAO-B inhibitory characteristics of a series of sixteen pyridazinone derivatives that were synthesized. The majority of the chemicals inhibited MAO-B more effectively than MAO-A. The lead compounds TR2 and TR16 have been shown to be competitive and reversible MAO-B inhibitors in kinetic and reversibility experiments. The PAMPA test revealed that the lead candidates have a high level of CNS permeability. According to docking studies, the strong inhibitory efficiency of compounds TR2 and TR16 is due to the possibility of interaction with important and selective residues of E84 or Y326 in MAO-B. These findings are consistent with the experimental data. The compounds' pharmacological similarity was also shown by the favorable bioavailability radar.