Essential Oil Content and Compositional Variability of Lavandula Species Cultivated in the Mid Hill Conditions of the Western Himalaya

The increase in the utilization of Lavandula essential oil in industries led to an impressive rise in the demand for quality essential oils. However, a post-harvest drying of Lavandula species can be a decisive factor to determine the quantity and quality of essential oil. The study was conducted in western Himalayan conditions to assess the essential oil content and composition of two Lavandula species viz., lavender (Lavandula angustifolia Mill.), and lavandin (Lavandula × intermedia Emeric ex Loisel), at four different drying duration (0 h, 24 h, 48 h and 72 h after the harvest). The higher growth attributes viz., plant height (71.7 cm), ear length (8.8 cm), number of spikes (18.1), and number of flowers per ear (47.5) were higher in lavandin, while the number of branches (17.1) was higher in lavender. Essential oil content (%) and moisture reduction (%) were significantly higher at 72 h than at 0 h. The major components of lavender and lavandin essential oil were linalool (33.6–40.5%), linalyl acetate (10.8–13.6%), lavandulyl acetate (2.8–14.5%), and linalyl propionate (5.3–14.1%) in both the Lavandula species. There was a decreasing trend in linalool and an increasing trend in linalyl acetate content in lavandin, with an increase in drying duration up to 72 h; while in lavender, no regular trend was observed in linalool and linalyl acetate content. It was observed that linalool and linalyl acetate levels were the highest at 24 and 0 h of drying in lavender and lavandin, respectively, and essential oil extraction can be done according to the desire of the constituent at varied drying duration.


Introduction
The usage of medicinal and aromatic plants (MAPs) since prehistoric times have been well known, but these are recently recognized around the world as economical crops for high-valued essential oil [1]. The essential oil-producing plants belong to different genera with families including Lamiaceae, Asteraceae, Apiaceae, Alliaceae, Rutaceae, Poaceae, and Myrtaceae. One of the important plant families is "Lamiaceae", which has been reported to contain 236 genera and 6900-7200 species. The genus Lavandula is of major significance inthe Lamiaceae family, covering more than 40 species and many subspecies. The principal cultivated species for aromatic oils are fine lavender (Lavandula angustifolia Mill.), spike lavender (L. latifolia), and lavandin (L. × intermedia Emeric ex Loisel), a sterile hybrid of L. angustifolia × L. latifolia [2]. The main production country of lavender is Bulgaria, followed by France; the top producer of lavandin essential oil is France, followed by Spain [3]. The essential oil composition of Lavandula species almost has a similar chemical profile, but the components are usually present in varied proportions. The presence of a few essential oil components, viz., camphor, linalool, and linalyl acetate is the common criterion for quality determination of essential oil of lavandin [4]. The lavender essential oil contains linalyl acetate (25-45%), linalool (25-38%), and camphor (0.5-1.0%), while lavandin essential oil contains linalyl acetate (28-38%), linalool (24-35%), and camphor

Essential Oil Composition
The essential oil of Lavandula species led to the identification of sixteen constituents representing 92.3-95.5% of the total volatiles. The ANOVA of the data demonstrated sta tistically significant (p = 0.05) effects of Lavandula species and drying hours on content (% and constituents (area%) of essential oil ( Table 1). The identified constituents were simi lar in both the Lavandula species (except 1,8-cineole, isophorone, caryophyllene, del ta-cadinene, and caryophyllene oxide), and differed only in the area of distribution (Ta ble 2). The major contribution (mean) was of linalool (33.6-40.5%), followed by linaly acetate (10.8-13.6%), lavandulyl acetate (2.8-14.5%), and linalyl propionate (5.3-14.1%) in both the Lavandula species. Significantly higher 3-octanone, linalool, cryptone, linalyl ac etate, lavandulyl acetate, neryl acetate, and caryophyllene were recorded in lavende compared to lavandin. On the other hand, (Z)-β-ocimene, isophorone, terpinen-4-ol, and linalyl propionate were significantly higher in lavandin compared to lavender. During different drying hours, the content of 3-octanone, linalool, isophorone, and lavanduly acetate were significantly higher in 0 drying hours, while 3-octanone remained at pa with 24 drying hours. We have not recorded any regular trend in the occurrence of es sential oil constituents with an increase or decrease in drying hours for Lavandula species Figure 1. Effect of Lavandula species and drying hours on moisture reduction (%) and essential oil content (%). Note: Dissimilar letters represent significant differences in moisture reduction (%) essential oil content (%) at p = 0.05.

Essential Oil Composition
The essential oil of Lavandula species led to the identification of sixteen constituents, representing 92.3-95.5% of the total volatiles. The ANOVA of the data demonstrated statistically significant (p = 0.05) effects of Lavandula species and drying hours on content (%) and constituents (area%) of essential oil ( Table 1). The identified constituents were similar in both the Lavandula species (except 1,8-cineole, isophorone, caryophyllene, deltacadinene, and caryophyllene oxide), and differed only in the area of distribution ( Table 2). The major contribution (mean) was of linalool (33.6-40.5%), followed by linalyl acetate (10.8-13.6%), lavandulyl acetate (2.8-14.5%), and linalyl propionate (5.3-14.1%) in both the Lavandula species. Significantly higher 3-octanone, linalool, cryptone, linalyl acetate, lavandulyl acetate, neryl acetate, and caryophyllene were recorded in lavender compared to lavandin. On the other hand, (Z)-β-ocimene, isophorone, terpinen-4-ol, and linalyl propionate were significantly higher in lavandin compared to lavender. During different drying hours, the content of 3-octanone, linalool, isophorone, and lavandulyl acetate were significantly higher in 0 drying hours, while 3-octanone remained at par with 24 drying hours. We have not recorded any regular trend in the occurrence of essential oil constituents with an increase or decrease in drying hours for Lavandula species.

Grouped Constituents Classes of Essential Oil
The grouped essential oil constituents were also significantly (p = 0.05) affected by Lavandula species and drying hours ( Table 2). Significantly higher (mean) monoterpenes (50.10%) and oxygenated sesquiterpenes (1.13%) were recorded in lavandin, while sesquiterpenes were higher in lavender, but oxygenated monoterpenes were not significantly affected by Lavandula species. The drying hours affected the grouped constituent content distribution, but no regular trend was observed with an increase or decrease in drying hours (Table 2). Moreover, significantly higher (mean) monoterpenes (52.72%) and sesquiterpenes (3.52%) were recorded at 24 h of drying compared to 48 h and 72 h of drying, respectively. On the other hand, significantly higher oxygenated monoterpenes (47.57%) and oxygenated sesquiterpenes (0.85%) were recorded in 48 h of drying compared to 24 h of drying.

Principal Component Analysis
The identified essential oil constituents (>2% area) of Lavandula species were considered as variables and subjected to principal component (PC) analysis to evaluate the relation between Lavandula species and drying hours levels ( Figures 2 and 3). The analysis revealed 99.99% of the total variability explained by PC1 and PC2 ( Figure 2a). The PC analysis of lavender separated the drying levels 0, 24, 48, and 72 h in PC1 and PC2 by presenting positive contribution and strong relationships with (Z)-β-ocimene, linalool, linalyl propionate, neryl acetate, and caryophyllene. The present PCA findings of lavender showed that the first three PCs were decidedly enlightening with 6.98, 3.02, and 0.01 as eigen values; and consequently gave about 99.99% of the overall essential oil compounds' variance. The observed score plot of lavender possibly would be divided into four distinct clusters ( Figure 2a and Table 3). The clusters were prepared from the area (%) of essential oil components through PCA software and the treatments with almost similar or related essential oil composition fall together or in the nearby location of the X-axis and Y-axis planes of the graph. The treatments that fall together were then clubbed together and given a cluster number; then, the content of essential oil components in the treatments which fall under a particular cluster was added to form the area%, as given in Table 3. Cluster I, II, III, IV corresponded to 0, 24, 48, and 72 h, respectively. Cluster I explained a higher percentage of lavandulyl acetate (30.8%), while cluster II explained a higher percentage of linalool (45.0%). Similarly, a higher percentage of linalyl acetate (34.2%) was explained by cluster III, while cluster IV observed an intermediate percentage of linalool (40.0%); this compositional variability of the treatments made them fall separate from each other and form distinctive clusters ( Figure 2a and Table 3). Among all variables in lavender, 3-octanone, (Z)-β-ocimene, linalool, linalyl propionate, lavandulyl acetate, neryl acetate, and caryophyllene showed a positive association, while terpinen-4-ol, cryptone, and linalyl acetate showed negative association in PC1. Similarly, in PC2, (Z)-β-ocimene, linalool, terpinen-4-ol, cryptone, linalyl propionate, linalyl acetate, neryl acetate, and caryophyllene showed a positive association, while 3-octanone and lavandulyl acetate showed a negative association (Figure 2b).
Similarly, the PC analysis of lavandin separated the drying levels 0, 24, 48, and 72 h in PC1 and PC2 by screening positive contribution and good relationships with linalool and isophorone. The present PCA findings of lavandin showed that the first three PCs were definitely helpful with 7.1, 1.1, and 0.01 as eigenvalues, and thus contributed to 99.36% of total essential oil compounds' variance. Three distinct clusters for lavandin ( Figure 3a and Table 3) emerged from the PC analysis of constituents. Cluster I corresponds to 0 h with the lowest linalyl propionate percentage (16.9%), while cluster II corresponds to 24 h of drying with the lowest linalool (33.0%) and highest linalyl propionate (21.9%) percentage. However, cluster III included 48 and 72 h of drying which corresponds to the highest linalool (63.5%) and highest linalyl acetate (36.2%) percentage ( Figure 3a and Table 3). Among all variables in lavandin, 3-octanone, (Z)-β-ocimene, linalool, isophorone, and linalyl propionate showed a positive association, while terpinen-4-ol, cryptone, linalyl acetate, and lavandulyl acetate showed negative association in PC1. Similarly, in PC2, linalool, isophorone, and lavandulyl acetate showed a positive association, while 3-octanone, (Z)-β-ocimene, cryptone, linalyl propionate, and linalyl acetate showed a negative association (Figure 3b). On the whole, a significant difference in essential oil constituents was recorded in Lavandula species and varied post-harvest drying hours. explained a higher percentage of linalool (45.0%). Similarly, a higher percentage of linalyl acetate (34.2%) was explained by cluster III, while cluster IV observed an intermediate percentage of linalool (40.0%); this compositional variability of the treatments made them fall separate from each other and form distinctive clusters (Figure 2a and Table 3). Among all variables in lavender, 3-octanone, (Z)-β-ocimene, linalool, linalyl propionate, lavandulyl acetate, neryl acetate, and caryophyllene showed a positive association, while terpinen-4-ol, cryptone, and linalyl acetate showed negative association in PC1. Similarly, in PC2, (Z)-β-ocimene, linalool, terpinen-4-ol, cryptone, linalyl propionate, linalyl acetate, neryl acetate, and caryophyllene showed a positive association, while 3-octanone and lavandulyl acetate showed a negative association (Figure 2b).       Morphological characteristics, viz., plant height, ear length, and number of spikes were slightly higher in lavandin, while a considerable increase (23.31%) in flower number per ear was observed in lavandin. The plant morphological characters of Lavandula cultivars in semi-arid climatic conditions of Isparta Province were earlier studied and recorded higher growth and yield attributes in cultivars of lavandin as compared to lavender [5]. Corroborating to our findings, a similar growth attribute was reported in lavender when grown in subtropical conditions in Italy [31].
The highest moisture reduction was recorded in 72 h of drying in both Lavandula species because the rate of drying was much faster from 0 to 24 h, but the rate gradually decreased with later drying hours from 24 to 72 h. Similar findings in moisture reduction have been reported in Pistacia lentiscus L. leaves with an increase in drying time [32]. The highest essential oil content was obtained in 72 h of drying and the findings were in good agreement with findings in Eugenia uniflora L. leaves where higher essential oil content was recorded in later drying of 48 h [33]. The volatile oil content has been reported to be affected by the drying time, drying method, temperature, and plant species because the moisture level of samples dried for different times can be highly variable and affected essential oil content [34]. For instance, the highest essential oil content was recorded in 72 h of drying when moisture reduction was maximum (Figure 1) in samples compared to other drying hours. In addition, some important physiological activity (enzymatic reactions) that continue after crop harvest was responsible for the biosynthetic activity of terpenes and resulted in an increase in essential oils content [35] reported within the limits as in earlier findings in Lavandula species [36,37]. A prolonged drying time (up to 8 days) decreased the essential oil content of kaffir lime (Citrus hystrix DC), but shorter drying from zero to four days increased the essential oil content [38], which is similar to present findings up to 72 h of drying. The shorter shade drying period (first seven days) increased essential oil content, but decreased with increased drying duration [32].

Essential Oil Composition
The identified sixteen essential oil constituents were significantly influenced by Lavandula species and post-harvest drying hours ( Table 1). The major contribution in the essential oil of both Lavandula species was linalool and linalyl acetate, and overall content ranged from 32.9% to 45.0% and 0.6% to 34.2%, respectively. The earlier findings have also reported linalool and linalyl acetate as 10.0-57.5% and 4-55%, respectively, in the essential oil of L. angustifolia [36], while linalool, linalyl acetate, and borneol were shown to be major essential oil constituents in lavandin [5].
The presence of linalool and linalyl acetate is the criterion for the quality of lavender essential oil and is highly demanded in the international markets because of their sedative and narcotic effects [39]. The content of camphor in the present finding was even less than 0.1% among the drying hours and not shown in Table 1,which corroborated with the International Organization for Standardization (ISO 3515:2002) for camphor content in lavender, i.e., lower than 0.5%. In the present study, the lavender and lavandin recorded higher linalool and linalyl acetate at 48 h of drying and were according to essential oil standards (ISO 3515:2002). Moreover, long-term storage of dried flowers reduced the total constituents' content viz., linalool, linalyl acetate, lavandulyl acetate, and terpinen-4-ol between the lavender varieties [37]. The constituents of L. angustifolia and L. × intermedia obtained from essential oil in the present finding ranged from 3-octanone: 1.  [40], except for a few essential oil constituents. The variability in essential oil depends on several factors, viz., environment, climate, geographical location, season, genetic makeup, chemotype, plants' nutritional status, the extraction process, and the plant part used [41].
There was a considerable decrease in the content of lavandulyl acetate with an increase in drying hours from 0 to 48 h, and again showing an increase at 72 h of drying in both Lavandula species. The decreased lavandulyl acetate can be explained as changes that have resulted because of the conversion of lavandulyl and linalyl esters to alcohols and molecular reshuffling [37]. The statistical analysis of the drying duration on the essential oil composition does not confirm the increasing or decreasing trends for all essential oil constituents. However, it can be assumed that if a longer drying period was evaluated, differences in the composition of essential oil would be confirmed statistically. The constituents of lavandula essential oil are not only linalool, linalyl acetate, and lavandulyl acetate, but a complex of up to 60 monoterpenes and sesquiterpenes [17]. The interactions of all of these substances with their moisture content at the time of hydrodistillation determine the resulting chemical composition of the essential oil.
Moreover, in the process of hydrodistillation, the degradation/hydrolysis of thermolabile compounds/water-sensitive compounds has been observed, and the esters are generally hydrolyzed by the release of H + ions from water to analogous acids and alcohols. The pH of water is often reduced during hydrodistillation and essential oil components like esters are sensitive to hydrolysis, while others like acyclic monoterpene hydrocarbons and aldehydes are susceptible to polymerization because of low pH [42]. As the terpenoids are volatile and thermolabile, they may be easily oxidized or hydrolyzed depending on their respective structure. The chemical composition of essential oils is additionally dependent on the conditions of storage of the plant material, processing conditions upon distillation, and after extraction storage conditions [43]. This degradation and rearrangement resulted in the increase and decrease of essential oil constituents of Lavandula species in the present study. The conversion of a few essential oil constituents such as geranyl acetate to geraniol, neral, and geranial was also observed during the storage of ginger (Zingiber officinale R.) [44]. The data obtained in the present study was valuable to respond to the issue of whether there is any loss in essential oil constituents during post-harvest drying of Lavandula species. The interactions among all the constituents determine the resultant chemical properties of the essential oils, and thus provide evidence of whether there was a loss of essential oil composition at the initial and at the final stage of drying.
The Lavandula species studied in the present study recorded a higher percentage (mean) of monoterpenes and oxygenated sesquiterpenes (1.13%) in lavandin (50.10%), and higher oxygenated monoterpenes (42.64%) and sesquiterpenes (3.78%) in lavender. This diversity in mono-and sesquiterpene can be associated with the catalytic activity of the enzymes of the terpene synthase (TPS) family. The complex spatial and temporal arrangement of TPS members during plant development and biotic/abiotic stresses may be responsible for the expression profile of terpenes [45]. The activities of TPS were shown to be regulated at the transcription level [45], and in peppermint, the transcription of TPS and numerous terpene biosynthetic genes get modified during the development stage [46] and regulate terpene biosynthesis [47]. Similar to our findings, hydrodistillation usually produced essential oil with chemical composition chiefly described by the presence of oxygenated monoterpenes viz., linalool, linalyl acetate, 1,8-cineole, and monoterpenoids, viz., lavandulyl acetate. Monoterpenes viz., α-β-pinene and β-ocimene; sesquiterpenes, viz., caryophyllene; and oxygenated sesquiterpenes viz., caryophyllene oxide were represented as minor compounds [48], while some described molecules of several chemical derivatives (phenolic acids and flavonoid) [49,50]. Monoterpenes and sesquiterpenes are the most recurrently studied hydrocarbons in lavender essential oil due to their abundance and importance in the fragrance and flavor industry [51]. Corroborating to our findings, lavender and lavandin produced the majority of oxygenated monoterpenes followed by monoterpene hydrocarbons with linalool and linalyl acetate as major constituents [52,53].

Experimental Site
An experiment was executed in 2020 in the CSIR-IHBT (Council of Scientific and Industrial Research, Institute of Himalayan Bioresource Technology), Palampur, Himachal Pradesh (HP), India; situated at an altitude of 1390 m above mean sea level (amsl) (32 • 11 39 N latitude and 76 • 56 51 E longitude) in a subtropical climate. Weather parameters viz., minimum and maximum temperature ( • C), and relative humidity (RH%) during the third year of crop growth are depicted in Figure 4. The minimum temperature ranged from 9 to 26 • C, while the maximum temperature ranged from 19 to 37 • C during the crop harvesting year. Mean relative humidity (%) ranged from 63% to 98% during crop growth.

Treatment Details
The experiment consists of eight treatments, two Lavandula species viz., common lavender (L. angustifolia Mill.) and lavandin (Lavandula × intermedia Emeric ex Loisel), and four drying hours, i.e., 0 h, 24 h, 48 h, and 72 h, with three replications. Lavandula species were raised in polythene tunnel conditions, planted in October 2016, and harvested in May 2020. Both the Lavandula species were planted in a polythene tunnel because the experimental location is a high rainfall area, and thus, the species were protected from water logging conditions in the polythene tunnel. The polythene tunnel was 23.30 × 3.90 m for lavender and 14.50 m × 1.80 m for lavandin. The soil was a mixture of sand, soil, and decomposed farmyard manure (FYM at a rate of 20 kg m −2 ).

Treatment Details
The experiment consists of eight treatments, two Lavandula species viz., common lavender (L. angustifolia Mill.) and lavandin (Lavandula × intermedia Emeric ex Loisel), and four drying hours, i.e., 0 h, 24 h, 48 h, and 72 h, with three replications. Lavandula species were raised in polythene tunnel conditions, planted in October 2016, and harvested in May 2020. Both the Lavandula species were planted in a polythene tunnel because the experimental location is a high rainfall area, and thus, the species were protected from water logging conditions in the polythene tunnel. The polythene tunnel was 23.30 × 3.90 m for lavender and 14.50 m × 1.80 m for lavandin. The soil was a mixture of sand, soil, and decomposed farmyard manure (FYM at a rate of 20 kg m −2 ).

Experimental and Plant Details
The soil samples collected at 0-20 cm depth from the experimental tunnels were pooled, dried, sieved (2 mm sieve), and stored at room temperature until soil physicochemical analysis. The soil of the experimental polythene tunnel area was clay loam in texture, acidic pH ( , and 72 h after harvesting, and then essential oil was extracted. The minimum and maximum daily temperature during five drying hours were between 14-20 • C and 29-33 • C, respectively, while humidity ranged from 49-73% during crop drying conditions. The harvested biomass (weight: 1 kg) of both the Lavandula species was spread over a clean and dry surface area (1 m 2 ) for each drying hour treatment.

Plant Growth Investigation
Plant height was measured in centimeters (cm) by randomly selecting the plants (five plants per species) from the base of the ground to the tip of the main stem by using measuring tape; then, the average height of the plant was determined. The branch number and number of spikes per plant arising from the main stem were determined by counting the stems manually, and the average value was determined. Ear length per spike and number of flowers per ear were also determined through scale and manual counting, respectively, at harvest.

Identification and Determination of Essential Oil Components
From each treatment, fresh samples were harvested, and then brought to the laboratory and hydrodistilled after 0, 24, 48, and 72 h of drying in triplicates for 4 h in the Clevengertype apparatus [54]. The essential oil content was calculated as volume/weight (%), i.e., volume (mL) of essential oil obtained per weight (g) of flowers and then dried with anhydrous sodium sulfate (Na 2 SO 4 ) to remove any water droplet. The essential oil was collected in a glass container and stored at 4 • C before analysis. GC and GC/MS analysis were performed in triplicates through a flame ionization detector (FID) on Shimadzu GC 2010 gas chromatography and QP2010 (Shimadzu Corp., Tokyo, Japan) fitted with an AOC 5000 auto-injector. The auto-injector comprises a 30 m long ZB-5 MS capillary column with a 0.25 mm i.d. and a 0.25-µm thick film (SGE International, Ringwood, Australia). Essential oil (10 µL) was dissolved in 2 mL of dichloromethane and auto-injected with 2 µL volume in split mode. Carrier gas (nitrogen) was used with a 1.05 mL min −1 flow rate; an oven temperature of 70 • C for 3 min was used, which was afterward raised to 220 • C for 5 min at the rate of 4 • C min −1 , and the injector and detector temperature was programmed at 220 • C and 250 • C, respectively. The temperature programming, injection volume, and carrier gas conditions utilized for performing GC and GC/MS were according to the mentioned procedure in Matricaria chamomilla L. [55]. To identify the compounds from GC peak areas, a series of hydrocarbons was utilized for retention index (RI) estimation. The essential oil components were identified after matching the experimental RIs with the RIs in the literature [56]. Furthermore, the components were identified by matching the minimum mass spectral fragmentation pattern of the components with the National Institute of Standards and Technology (NIST) library [57].

Statistical Analysis
After chemical evaluation, data were analyzed using the analysis of variance (ANOVA) technique for factorial Complete Random Design (CRD). Fisher's least significant difference (LSD) test was performed for the least significant testing and regarded statistically significant at p = 0.05. The essential oil constituents were subjected to multivariate principal component analysis (PCA software PAST 3) to assess the expression of treatments on essential oil constituents.

Conclusions
The current experiment assessed the changes in the essential oil content and composition after 0, 24, 48, and 72 h of post-harvest shade drying in Lavandula species under mid hill conditions of the western Himalaya. Present findings confirmed inter-specific variability in Lavandula; specific drying duration ought to be taken into consideration to optimize essential oil production and industrial utilization. Lavandula species recorded significantly higher essential oil content at 72 h of drying, and acceptable quality (ISO 3515:2002 and ISO 8902:2009) of essential oil in terms of linalool and linalyl acetate content. During drying duration, lavandin recorded a decreasing trend in the linalool content and an increasing trend of linalyl acetate, while lavender recorded an irregular trend in linalool and linalyl acetate with drying duration. The differences in essential oil content and chemical constituents were statistically confirmed, although the confirmation was for a short-term drying duration of up to 72 h of monitoring. Nevertheless, investigations should be confirmed by drying the Lavandula species for a longer duration as the content of essential oil increased up to 72 h of drying and can be carried out for a longer duration to evaluate essential oil content and composition. However, it is necessary to conduct further research aimed at developing improved agronomic practices and post-harvest management for improved yield and desired quality of essential oil.  Acknowledgments: The authors are grateful to the Director, CSIR-IHBT, Palampur, India, for providing necessary facilities during the study. The authors also thank to Kuldip Singh, Shiv Kumar, and Vinod Bhatt for providing support during the research work. This is CSIR-IHBT communication number 4957.