Mucuna pruriens Seed Aqueous Extract Improved Neuroprotective and Acetylcholinesterase Inhibitory Effects Compared with Synthetic L-Dopa

L-dopa, a dopaminergic agonist, is the gold standard for the treatment of Parkinson’s disease. However, due to the long-term toxicity and adverse effects of using L-dopa as the first-line therapy for Parkinson’s disease, a search for alternative medications is an important current challenge. Traditional Ayurvedic medicine has suggested the use of Mucuna pruriens Linn. (Fabaceae) as an anti-Parkinson’s agent. The present study aimed to quantify the amount of L-dopa in M. pruriens seed extract by HPLC analysis. The cytotoxicity and neuroprotective properties of M. pruriens aqueous extract were investigated by two in vitro models including the serum deprivation method and co-administration of hydrogen peroxide assay. The results showed the significant neuroprotective activities of M. pruriens seed extracts at a concentration of 10 ng/mL. In addition, the effects of L-dopa and M. pruriens seed extract on in vitro acetylcholinesterase activities were studied. M. pruriens seed extract demonstrated acetylcholinesterase inhibitory activity, while synthetic L-dopa enhanced the activity of the enzyme. It can be concluded that the administration of M. pruriens seed might be effective in protecting the brain against neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases. M. prurience seed extract containing L-dopa has shown less acetylcholinesterase activity stimulation compared with L-dopa, suggesting that the extract might have a superior benefit for use in the treatment of Parkinson’s disease.


Plant Sample Collection and Identification
The seeds of M. pruriens were purchased from a local market in Kanchanaburi province and kindly provided by Chao-Phraya Abhaibhubejhr Hospital Foundation. The plant materials were compared with the authentic specimens at the Bangkok Herbarium (BK), Botany Section, Botany and Weed Science Division, Department of Agriculture. The voucher specimen (RSU-MP-KP01) was kept in the Department of Pharmacognosy, College of Pharmacy, Rangsit University for future reference.

Plant Extraction
The seeds of M. pruriens (10 kg) were roasted by a roasting drum for 30 min at a temperature of 180 • C. The roasting temperature was measured by a sensor located in the drum. The roasted seeds were crushed to fine powders by passing through a stainless-steel sieve with a nominal mesh aperture of 180 µm. The fine powders were extracted with boiling water at 100 • C. The ratio of fine powder to hot water was 1:7. The water extract was mixed using the heating and stirring method with the heater and homogenizer for 15 min. After filtration with the white cloth, the filtrate was then freeze-dried to remove the solvent for 24 h. Finally, the concentrate crude extract was obtained.

HPLC Analysis of L-Dopa in M. pruriens Extract
Isocratic HPLC analysis was performed to analyze the amount of L-dopa remaining in the extract using Perkin Elmer series 200, USA, equipped with auto-sampler and a UV-Vis detector. The injection volume was 20 µL for all samples. The mobile phase for elution was 0.1 M KH 2 PO 4 (pH 2.5). Samples were eluted on ACE-129-2546 (250 mm × 4.6 mm) C 18 column with a flow rate of 1.0 mL/min. L-dopa peak was integrated at the wavelength of 283 nm. A stock solution of L-dopa was prepared to obtain L-dopa concentration of 1 mg/mL. The stock solution was diluted to 10, 20, 30, 40, and 50 µg/mL in 0.1 N HCl. M. pruriens aqueous extract was weighed and dissolved in 0.1 N HCl. Samples were then filtered through Whatman filter paper and 0.45 µm membrane filter.

Quantitative Analysis of Total Phenolic Content
Total phenolic content in M. pruriens seed extract was determined by the Folin-Ciocalteu reaction. Gallic acid solution (3.9-125 µg/mL) and M. pruriens seed extract solution (2 mg/mL) were mixed with 10% v/v of Folin-Ciocalteu phenol reagent (100 µL) for 1 min. After 4 min of incubation, 7.5% w/v Na 2 CO 3 solution (50 µL) was added, and the mixture was further incubated in the dark at room temperature for 2 h. The absorbance was read using a UV-visible spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA) at a wavelength of 765 nm. Total phenolic contents were calculated from the gallic acid standard curve. Data were expressed as mg/g gallic acid equivalents (GAE) of dry crude extract.

Quantitative Analysis of Total Flavonoid Content
The total flavonoid content in M. pruriens seed extract was determined by the aluminum chloride colorimetric method. Quercetin solution (3.9-500 µg/mL) and M. pruriens seed extract solution (2 mg/mL, 100 µL/well) were mixed with 5% NaNO 2 (30 µL) and incubated for 5 min. Aluminum chloride (2% w/v, 50 µL) was added and incubated for 6 min followed by 10 min of incubation with 1 N NaOH (50 µL). The absorbance was measured at a wavelength of 510 nm with a UV-Vis spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA). Total flavonoid contents were calculated from quercetin and were expressed as mg/g quercetin equivalents (QE) of dry crude extract.

Neuronal Cell Viability Assay
The cytotoxicity assay was carried out on P19-derived neurons cultured in a 96-well plate. After 14 days of differentiation process, the α-MEM supplemented with 10% FBS, 10 µM Ara-C, and 1% antibiotics-antimycotic solution (P19SM) was removed, and DMSO solutions of the sample, diluted with the P19SM were added to give the concentrations of 10,000, 1000, 100, 10, and 1 ng/mL [20][21][22][23][24]. The 0.5% v/v DMSO in P19SM was used as control. The cells were incubated for 18 h at 37 • C. Then 150 µL of the medium was removed, and 50 µL of XTT solution (1 mg/mL XTT in 60 • C α-MEM + 25 µM PMS) was added. After incubation at 37 • C for 4 h, 100 µL of PBS (phosphate buffer saline solution) pH 7.4 was added. The OD value was determined on a microplate reader at 450 nm. The data were expressed as the average % cell viability ± SE (n = 3, each n was run in triplicate). The samples that enhanced survival of cultured neurons more than control (0.5% v/v DMSO in the medium) will be further investigated for their neuroprotective ability.

Serum Deprivation Method
The DMSO solution of the extracts diluted with the α-MEM supplemented with 1% antibiotics-antimycotic solution and 10 µM Ara-C without FBS were added to give the final concentration of the extract at concentration that enhanced survival of cultured neurons more than control. The 0.5% v/v DMSO in the completed medium (P19SM, α-MEM supplemented with 1% antibiotics-antimycotic solution and 10 µM Ara-C with 10% v/v FBS) was used as control. The 0.5% v/v DMSO in α-MEM supplemented with 10 µM Ara-C, and 1% antibiotics-antimycotic solution without FBS was used to make oxidative stress condition. The cells were incubated for 18 h at 37 • C. Cell viability was assayed by XTT reduction method. The data were expressed as the average % cell viability ± SE (n = 3) [10][11][12][13][14]. Quercetin at concentration of 1 nM was used as positive control [26].

Co-Administration of H 2 O 2 Assay
The DMSO solution of the extracts diluted with P19SM plus 10 µM Ara-C was added to give the final concentration of the extract at a concentration that enhanced survival of cultured neurons more than control. The 0.5% v/v DMSO in P19SM was used as control. The 5 mM H 2 O 2 in P19SM plus 10 µM Ara-C was used to make oxidative stress condition. The 5 mM H 2 O 2 and the extracts in P19SM plus 10 µM Ara-C were added together for coadministration assay. The cells were incubated for 18 h at 37 • C. Cell viability was assayed by XTT reduction method. The data were expressed as the average % cell viability ± SE (n = 3) [9]. Quercetin at concentration of 1 nM was used as positive control [26].

Acetylcholinesterase (AChE) Activity Assay
The effects of L-dopa and M. pruriens seed extract on the acetylcholinesterase activity were measured using a colorimetric assay based on an Ellman method. Briefly, samples (50 µL) were mixed with acetylcholinesterase enzyme (50 µL) in a 96-well plate. The assay buffer (50 µL) was used as a blank. The reaction was initiated by the addition of 1x substrate mix solution to the sample and blank wells. The hydrolysis of this substrate was immediately monitored by measuring the absorbance at a maximum wavelength of 412 nm in kinetic mode at 1 min-interval for 20 min. The formation of yellow 5-thio-2nitrobenzoate anion was detected as the result of the reaction of DTNB with thiocholine, released by the enzymatic hydrolysis of acetylthiocholine iodide. The enzymatic activity was calculated as a percentage of the velocity of the reaction with and without the extract. The velocity of the reaction was determined by constructing a kinetic curve between the absorbance and the incubation time. The slope was in units of O.D./min. The absorbance was calculated from the following equation.
where A is the absorbance (in O.D), ε is the extinction coefficient (13,600 L/molxcm), I is the path length of a 96-well plate, and c is the AChE activity in units (µmole.min).
To calculate the enzymatic activity (units/liter), the following equation was used.

Statistical Analysis
The average viability of the neurons was statistically analyzed by one-way ANOVA and further analyzed by Fisher's LSD to compare the statistical significance between the control or oxidative stress conditions and experimental groups. Differences were considered significant only when the p-value was less than 0.05.

Yield of M. pruriens Aqueous Extract and L-Dopa Content in the Extract
The yield of crude aqueous extract of M. pruriens seeds was 15.02%. L-dopa content in the crude aqueous extract analyzed by HPLC analysis was 7.05 + 0.02%.

Total Phenolic and Total Flavonoid Content in M. pruriens Aqueous Extract
The gallic acid standard curve equation was expressed as y = 0.0173x + 0.1011, r 2 = 0.999. The average total phenolic content in the extract was 105.59 ± 0.84 mg gallic acid equivalent (GAE)/g crude dry extract. The total flavonoid content in M. pruriens seed extract determined by the aluminum chloride colorimetric method was expressed in quercetin equivalent amounts. The quercetin standard curve equation was y = 0.0013x + 0.0481, r 2 = 0.9977. The average total flavonoid content in the extract was 80.74 ± 0.51 mg quercetin equivalent (QE)/g crude dry extract.

Cell Viability Assay
P19-derived neurons culture was treated with an increasing concentration of M. pruriens seed aqueous extract and L-Dopa (0-10 µg/mL). The neuronal viability was determined by XTT assay. The results were expressed as % cell viability. The effective concentration of M. pruriens seed aqueous extract at 10 ng/mL (% neuron viability = 114.48 ± 19.25%) that enhanced the survival of cultured neurons more than control (% neuron viability of the control = 100.47 ± 0.67%) were further investigated for the neuroprotective ability ( Figure 1). Interestingly, L-dopa showed no enhancing cell viability effect on P19-derived neurons, suggesting that L-dopa was not responsible for the neuroprotective ability of M. pruriens. Therefore, only the extract was further investigated for its neuroprotective ability.
tract determined by the aluminum chloride colorimetric method was expressed in quercetin equivalent amounts. The quercetin standard curve equation was y = 0.0013x + 0.0481, r 2 = 0.9977. The average total flavonoid content in the extract was 80.74 ± 0.51 mg quercetin equivalent (QE)/g crude dry extract.

Cell Viability Assay
P19-derived neurons culture was treated with an increasing concentration of M. pruriens seed aqueous extract and L-Dopa (0-10 µg/mL). The neuronal viability was determined by XTT assay. The results were expressed as % cell viability. The effective concentration of M. pruriens seed aqueous extract at 10 ng/mL (% neuron viability = 114.48 ± 19.25%) that enhanced the survival of cultured neurons more than control (% neuron viability of the control = 100.47 ± 0.67%) were further investigated for the neuroprotective ability ( Figure 1). Interestingly, L-dopa showed no enhancing cell viability effect on P19derived neurons, suggesting that L-dopa was not responsible for the neuroprotective ability of M. pruriens. Therefore, only the extract was further investigated for its neuroprotective ability.

Serum Deprivation Method
P19-derived neurons culture was treated with 10 ng/mL of M. pruriens seed aqueous extract compared with toxic condition (0.5% v/v DMSO in α-MEM without serum) and positive control (1 nM quercetin). Cell viability was assayed by XTT reduction method. The results show significant cell viability in 10 ng/mL Mucuna (28.73 ± 1.90%) compared with the toxic condition (8.37 ± 0.73%) (Figure 2). The % neuron viability, when treated with 1 nM quercetin (positive control), was 34.73 ± 3.93%. No significant difference was

Serum Deprivation Method
P19-derived neurons culture was treated with 10 ng/mL of M. pruriens seed aqueous extract compared with toxic condition (0.5% v/v DMSO in α-MEM without serum) and positive control (1 nM quercetin). Cell viability was assayed by XTT reduction method. The results show significant cell viability in 10 ng/mL Mucuna (28.73 ± 1.90%) compared with the toxic condition (8.37 ± 0.73%) (Figure 2). The % neuron viability, when treated with 1 nM quercetin (positive control), was 34.73 ± 3.93%. No significant difference was found between the % neuron viability when treated with 10 ng/mL Mucuna and 1 nM quercetin.

Co-Administration of H 2 O 2 Assay
P19-derived neurons culture was co-treated with 10 ng/mL of M. pruriens seed aqueous extract and 5 mM H 2 O 2 . The cell viability was compared with the toxic condition (5 mM H 2 O 2 ). The cell viability was assayed by XTT reduction method. The results showed significant cell viability in 10 ng/mL M. pruriens seed extract co-treated with 5 mM H 2 O 2 (25.01 ± 2.66%), compared with the toxic condition (11.61 ± 0.50%) (Figure 3). The % neuron viability when co-treated with 1 nM quercetin (positive control) and 5 mM H 2 O 2 was 20.96 ± 3.67%. No significant difference was found between the % neuron viability when treated with 10 ng/mL M. pruriens seed extract and 1 nM quercetin.

Co-Administration of H2O2 Assay
P19-derived neurons culture was co-treated with 10 ng/mL of M. pruriens seed aqueous extract and 5 mM H2O2. The cell viability was compared with the toxic condition (5 mM H2O2). The cell viability was assayed by XTT reduction method. The results showed significant cell viability in 10 ng/mL M. pruriens seed extract co-treated with 5 mM H2O2 (25.01 ± 2.66%), compared with the toxic condition (11.61 ± 0.50%) (Figure 3). The % neuron viability when co-treated with 1 nM quercetin (positive control) and 5 mM H2O2 was 20.96 ± 3.67%. No significant difference was found between the % neuron viability when treated with 10 ng/mL M. pruriens seed extract and 1 nM quercetin.

Effects of L-Dopa and M. pruriens Seed Aqueous Extract on the Acetylcholinesterase Enzyme Activity
The effect of L-dopa and M. pruriens seed extract on acetylcholinesterase activity was investigated by the Ellman method, in which thiocholine produced by AChE reacted with 5, 5′-dithiobis(2-nitrobenzoic acid) to form a colorimetric product. The enzyme activity of the control without reaction with samples was 2.29 Units/L. One unit of AchE is the amount of enzyme that catalyzes the production of 1.0 µmole of tricholine per minute at pH 8.0 at room temperature. L-dopa was found to increase the activity of the acetylcho-

Effects of L-Dopa and M. pruriens Seed Aqueous Extract on the Acetylcholinesterase Enzyme Activity
The effect of L-dopa and M. pruriens seed extract on acetylcholinesterase activity was investigated by the Ellman method, in which thiocholine produced by AChE reacted with 5, 5 -dithiobis(2-nitrobenzoic acid) to form a colorimetric product. The enzyme activity of the control without reaction with samples was 2.29 Units/L. One unit of AchE is the amount of enzyme that catalyzes the production of 1.0 µmole of tricholine per minute at pH 8.0 at room temperature. L-dopa was found to increase the activity of the acetylcholinesterase enzyme in a dose-dependent manner, whereas M. pruriens seed extract showed inhibitory activity against the acetylcholinesterase enzyme. Figure 4 demonstrated a kinetic curve plotted between the absorbance of the Ellman reaction product and time. The slopes of each plot were further used to calculate the AChE activity. The AChE activity increased with increasing concentrations of L-dopa ( Figure 5). At the highest concentration of L-dopa (5 mg/mL), the AChE activity was activated up to 213%. In contrast, M. pruriens seed extract showed enzymatic inhibitory activity. The AChE inhibitory activity of M. pruriens seed extract was found to be 52.61% at an L-dopa equivalent concentration of 0.05 mg/mL. However, at the higher dose, the enzyme inhibitory effect was considerably lower to 11.02% at an L-dopa equivalent concentration of 3.5 mg/mL. This result was probably due to a higher concentration of L-dopa present in the extract.

Discussion
Parkinson's disease is a progressive neurodegenerative disorder resulting from the damaged dopaminergic neurons in the substantia nigra. The disease recently has no proven neuroprotective or neurorestorative therapy [27]. However, protecting neurons from premature cell death might be a promising alternative to managing this disease. Research directions include the investigation into animal models of the disease and the potential usefulness of gene therapy, stem cell transplant, neuroprotective agents, and herbals drugs.
The present study investigated the neuroprotective activities in P19-derived neurons using two models including the serum deprivation method and co-administration of hydrogen peroxide assay. Remarkably, the significant neuroprotective activities of standardized aqueous extracts of M. pruriens seed (L-dopa 7.05 ± 0.02 mg/100 g) were observed. The total phenolic compounds and total flavonoid content in M. pruriens seed aqueous extract were 106 GAE mg/g crude extract and 84 QE mg/g crude extract, respectively. Shin et al. have reported that L-dopa and pramipexol (a dopamine agonist) had comparable neuroprotective properties in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)treated Parkinson's disease animal models through modulation of cell survival and apoptotic pathway [28]. The total phenolic compounds have been shown to exert neuroprotective effects against hydrogen peroxide-induced oxidative damage by blocking reactive

Discussion
Parkinson's disease is a progressive neurodegenerative disorder resulting from the damaged dopaminergic neurons in the substantia nigra. The disease recently has no proven neuroprotective or neurorestorative therapy [27]. However, protecting neurons from premature cell death might be a promising alternative to managing this disease. Research directions include the investigation into animal models of the disease and the potential usefulness of gene therapy, stem cell transplant, neuroprotective agents, and herbals drugs.
The present study investigated the neuroprotective activities in P19-derived neurons using two models including the serum deprivation method and co-administration of hydrogen peroxide assay. Remarkably, the significant neuroprotective activities of standardized aqueous extracts of M. pruriens seed (L-dopa 7.05 ± 0.02 mg/100 g) were observed. The total phenolic compounds and total flavonoid content in M. pruriens seed aqueous extract were 106 GAE mg/g crude extract and 84 QE mg/g crude extract, respectively. Shin et al.
have reported that L-dopa and pramipexol (a dopamine agonist) had comparable neuroprotective properties in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated Parkinson's disease animal models through modulation of cell survival and apoptotic pathway [28]. The total phenolic compounds have been shown to exert neuroprotective effects against hydrogen peroxide-induced oxidative damage by blocking reactive oxygen species production and improving mitochondrial function [29]. Flavonoids have demonstrated neuroprotective effect via the inhibition of cholinesterase enzymes including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-secretase (BACE1) [30]. Therefore, our findings suggest that phenolic compounds and flavonoids in M. pruriens seed extract might play an important role in the neuroprotective effect against oxidative damage.
Regarding the serum deprivation method, the results demonstrated significant cell viability in 10 ng/mL M. pruriens extract (28.73 ± 1.90%) compared with the toxic condition (8.37 ± 0.73%). In addition, the results of the co-administration of hydrogen peroxide assay showed significant cell viability in 10 ng/mL M. pruriens extract (25.01 ± 2.66%) compared with the toxic condition (11.61 ± 0.50%). Our results agree with those of some previous studies. The evaluation of the anti-neuroinflammatory effects of M. pruriens extract was performed in murine microglia BV-2 cells. [16] M. pruriens extract was evaluated for neuroprotective effects in human neuroblastoma SH-SY5Y cells. The study showed that M. pruriens extract (at 1 µg/mL and 10 ng/mL) significantly reduced 6-hydroxydopamineinduced cytotoxicity and increased cell viability by 73.1 and 75.1%, respectively. The examination of ethanolic extract of M. pruriens on the level of nitric oxide in paraquat-induced Parkinson's disease mouse model and its subsequent contribution to lipid peroxidation results demonstrated that M. pruriens protects the dopaminergic neurons from the NO injury in substantia nigra [31]. The investigation of M. pruriens ethanolic seed extract in the Parkinsonian mouse model was performed [32]. A significant reduction in the activity of tyrosine hydroxylase positive neurons was observed in the substantia nigra region of the brain, after treatment with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Although the gold standard treatment of Parkinson's disease is L-dopa dopamine precursor, long-term L-dopa administration resulted in the development of serious side effects, including dyskinesia and the development of fluctuations in motor response. L-dopa-induced dyskinesia in Parkinson's disease patients significantly contributes to altered cholinergic signaling. Importantly, many studies showed that cholinergic receptor drugs including nicotine resulted in significant declines in L-dopa-induced dyskinesia [33][34][35]. Keeping the dose of L-dopa below 400 mg per day in early Parkinson's disease has been shown to reduce the risk of dyskinesia induction [36]. In this study, we showed that M. pruriens extract containing 7% natural L-dopa decreased the acetylcholine esterase stimulation compared with control. In contrast, the synthetic L-dopa significantly stimulated acetylcholinesterase activity. The results suggested that M. pruriens seed aqueous extract was probably able to modulate the cholinergic system and reduce L-dopa-induced dyskinesia and movement disorders resulting from L-dopa treatment. The present study demonstrated the neuroprotective activity of M. pruriens seed extract which may imply the therapeutic effects in various nervous system disorders including Parkinson's disease. Different mechanisms could underline this protection. Since M. pruriens seed extract showed neuroprotective activity against hydrogen peroxide-induced neurotoxicity, its proposed antioxidative therapeutics can be used for the protection of neuron injury induced by oxidative stress [37]. Serum deprivation-induced cell death is recognized as one of the standard models for the study of neurotoxicity. cGMP/PKG, PI13/Akt, and Bcl-2/Bax pathways have been shown to involve in the serum-deprivationinduced toxicity [38]. M. pruriens seed extract presented the potential of neuroprotective activity and thus may have a positive impact on aging and neurodegenerative diseases to retard the accelerated rate of neuronal degeneration.
M. pruriens seed extract contained substances including 5-hydroxytryptamine, alanine, arachidic acid, arginine, aspartic acid, behenic acid, beta-carboline, beta-sitosterol, bufotenine, choline, cis-12,13-epoxyoctadec-trans-9-cis-acid, cis-12,13-epoxyoctadec-trans-9-enoic-acid, cystine, gallic-acid, glutamic acid, glutathione, glycine, histidine, L-dopa, lecithin, leucine, linoleic acid, n, n-dimethyltryptamine, n, n-dimethyltryptamine-n-oxide, nicotine, phenyalanine, phosphorus, proline, protein, prurienidine, prurienine, saponins, serine, serotonin, threonine, tryptamine, tyrosine, valine, and vernolic acid, according to Dr. Duke's Phytochemical and Ethnobotanical Databases at Phytochemical Database, USDA-ARS-NGRL, Beltsville Agricultural Research Center [39]. Some of these compounds were AChE inhibitors, for example, physostigmine, which can enhance central levels of synaptic choline [40]. The aromatic amino acids such as tyrosine, phenylalanine, and tryptophan were identified as AChE inhibitors [41]. Pan et al. reported that flavonoid glycoside can inhibit AChE leading to a significant improvement in dyskinesia recovery rate in zebrafish [11]. Cognitive impairment and dementia are common complications of Parkinson's disease. Patients with Parkinson's disease with dementia often have significant cholinergic defects, which may be treated with cholinesterase inhibitors [42]. Several reports revealed that the use of an acetylcholinesterase inhibitor might improve cognitive function and reduce the risk of falls in patients with Parkinson's disease [43]. Therefore, M. pruriens seed extract with acetylcholinesterase inhibitory activity may be considered a prospective natural product for the treatment of Parkinson's disease, which may improve cognitive and motor functions of patients. This study reported the neuroprotective effect and anticholinesterase inhibition of M. pruriens seed aqueous extract, which contained large amounts of L-dopa (7%) along with other bioactive compounds including phenolic compounds and flavonoids. M. pruriens seed extract obtained in this study suggested more effectiveness and less toxicity than synthetic L-dopa for the treatment of Parkinson's disease. Our results were supported by Katzenschlager et al. showing the rapid onset of action and a long time without a concomitant increase in dyskinesias on M. pruriens seed powder formulation compared with L-dopa preparations in the long-term management of Parkinson's disease [14]. However, additional research is necessary to determine the agents responsible for other in vivo activities as well as the molecular mechanisms involved in their effects.

Conclusions
Long-term use of synthetic L-dopa can cause serious side effects such as dyskinesia and abnormal involuntary movement. A search for more effective treatment with lower side effects is significant for improving the quality of life of Parkinson's patients taking this drug. In this study, we reported the dual effects of M. pruriens seed extract on neuroprotective effect and acetylcholinesterase inhibitory activity at specific doses. Our findings suggested that M. pruriens seed extract is an attractive candidate for neuroprotection and preventing dyskinesia induced by cholinergic neuronal excitability in dopaminergic-depleted striatum.