On the Identification and Quantification of Ergothioneine and Lovastatin in Various Mushroom Species: Assets and Challenges of Different Analytical Approaches

In recent years, mushrooms have drawn the attention of agro-industries and food-industries as they were considered to be valuable natural sources of health promoting compounds such as β-glucans, ergothioneine, and lovastatin. The detection and quantification of such compounds by implementing reliable analytical approaches is of the utmost importance in order to adjust mushrooms’ cultivation conditions and maximize the production in different species. Toward this direction, the current study focuses on the comparison of ultraviolet–visible (UV–Vis) spectrometry and liquid chromatography–mass spectrometry (LC–MS) methods (a) by evaluating the content of ergothioneine and lovastatin in mushrooms and (b) by highlighting any possible substrate-based interferences that hinder the accurate determination of these two compounds in order to propose the technique-of-choice for a standardized bioactive compounds monitoring. For this purpose, mushrooms produced by three species (i.e., Agaricus bisporus, Pleurotus ostreatus, and P. citrinopileatus) on various cultivation substrates, namely wheat straw (WS), winery (grape marc (GM)), and olive oil (OL) by-products, were examined. Among the two applied techniques, the developed and validated LC–MS methods, exhibiting relatively short analysis time and higher resolution, emerge as the methods-of-choice for detecting ergothioneine and lovastatin in mushrooms. On the contrary, UV–Vis methods were hindered due to co-absorbance of different constituents, resulting in invalid results. Among the studied mushrooms, P. citrinopileatus contained the highest amount of ergothioneine (822.1 ± 20.6 mg kg−1 dry sample), whereas A. bisporus contained the highest amounts of lovastatin (1.39 ± 0.014 mg kg−1 dry sample). Regarding the effect of different cultivation substrates, mushrooms produced on OL and WS contained the highest amount of ergothioneine, while mushrooms deriving from GM-based substrates contained the highest amount of lovastatin.


Introduction
From the ancient years, mushrooms have been an integral part of many different culture diets such as the Asian, the European, and the American. Besides that, mushrooms were the basic ingredients of ethno-pharmacology and folklore medicine [1], since they exert several health-promoting properties, such as antioxidant, anti-inflammatory, anticancer, antimicrobial, anti-cholesterol, prebiotic, geno-protective, and immunomodulating activities, which are associated with specific compounds present in mushrooms, like of LC-MS methods are reflected particularly in ergothioneine and lovastatin analyses in dietary supplements, blood, and different human body tissues [26,27]. Nonetheless, this methodology has not been broadly applied to determine ergothioneine and lovastatin content in mushrooms where the presence of other co-extracted molecules may hinder the export of unbiased results.
One of the benchmarks of the present study was to assess the good performance of a simple, fast, low-cost, easily portable, in-the-field measurements-technique, like UV-Vis spectrophotometry, by (i) juxtaposing it with LC-MS outcomes and (ii) evaluating if the spectrophotometric approach could provide equally reliable and valid results since there are no reports that examine if UV-Vis is suitable or not for determining ergothioneine and lovastatin. Thus, two different analytical methodologies based on UV-Vis and LC-MS techniques were developed and compared in order to (a) appoint the method/techniqueof-choice for the analysis of bioactive compounds present in low concentrations, such as ergothioneine and lovastatin in mushrooms of three species (i.e., Agaricus bisporus, Pleurotus ostreatus, and P. citrinopileatus) and reveal any possible interferences that impede their determination including the use of various cultivation substrates (in the case of P. citrinopileatus), and (b) compare and identify mushroom species with higher content of ergothioneine and lovastatin. Overall, results of this study contribute to the appropriate selection of mushroom species along with the methodical choice of optimal cultivation conditions, which shape the final content of mushrooms in different bioactive compounds.

Results of Validation of LC-MS Methods
Analytical figures of merit of LC-MS analysis for ergothioneine and lovastatin are presented in Table 1. The results for repeatability, reproducibility, accuracy, and a matrix effect at three levels of concentration (quality control-QC samples) for both methods are summarized in Table 2. Since the present study does not address the analysis of biological samples or drugs, relative standard deviation (RSD%) values for repeatability and intermediate precision were satisfactory for both methods, not exceeding the maximum acceptable value of 15% [28]. Process recoveries for ergothioneine, regarding all mushroom species and substrates, ranged from 75.0% to 85.0%. Accordingly, extraction recovery for lovastatin varied from 63.0% to 79.2%.

Determination of Ergothioneine and Lovastatin Content of Mushrooms Produced in Conventional Substrates
Ergothioneine and lovastatin contents of different mushrooms, produced in conventional substrates (wheat straw and manure for A. bisporus, wheat straw for Pleurotus spp.), by using UV-Vis and LC-MS, is presented in Table 3.  According to LC-MS results, p-values indicated that ergothioneine contents differed significantly among all three species, offering a clear discrimination of the studied mushrooms based on their identity. In contrast, UV-Vis analysis sorted the investigated samples into two different groups based on ergothioneine concentration. Furthermore, it can be noted that ergothioneine concentration was an order of magnitude higher when UV-Vis method was applied, implying the existence of a possible positive error in spectrophotometric measurements.
Comparisons among the three mushrooms examined, which are demonstrated in both methods, P. citrinopileatus contained the highest amounts of ergothioneine, indicating that this particular species is more suitable for an ergothioneine-oriented mushroom production (Table 3). Several comparative studies report that Pleurotus species and P. ostreatus contain higher concentrations of ergothioneine compared to other edible mushrooms [23,29]. This is possibly associated with differences or changes in the biosynthetic pathways, which are responsible for the formation of ergothioneine, among mushroom species. Moreover, another important factor appears to be the bioavailability of compounds that can activate ergothioneine biosynthesis. For instance, the higher ergothioneine content of P. ostreatus may be ascribed to the higher levels of the precursor molecules histidine, cysteine, and methionine, that P. ostreatus contain, when compared to A. bisporus [30].
Similarly, based on lovastatin content measured by LC-MS, the samples were classified into three different groups, whereas, according to UV-Vis results, no statistically significant difference was observed among the mushrooms examined (p-values > 0.05) ( Table 3). As in the case of ergothioneine's spectrometric determination, lovastatin content using UV-Vis was over-estimated. Agaricus bisporus showed a higher amount of lovastatin than both Pleurotus species. However, A. bisporus and P. ostreatus lovastatin concentrations did not deviate much (Table 3). Nonetheless, the P. citrinopileatus strain used, which produced fruit bodies with a high content in ergothioneine, would not be proposed for the cultivation of lovastatin-rich mushrooms. These differentiations can be affected by many factors, such as the fungal strain and/or the substrate, since lovastatin production is crucially affected by the content of carbon and nitrogen [31], differences in gene adjustment, and bioavailability of compounds that can activate lovastatin biosynthesis, such as methionine, glutamate, glycine, and histidine [32].

Determination of Ergothioneine and Lovastatin Content of P. citrinopileatus Mushrooms from Different Substrates
Since P. citrinopileatus is a species that has not been thoroughly investigated, it was the one selected to be cultivated not only in the commonly used wheat straw (WS) substrate, but also in two "non-conventional" cultivation substrates, namely grape marc (GM) and olive by-products (OL). Ergothioneine and lovastatin content in the derived mushrooms were measured by using UV-Vis and LC-MS (Table 4). Table 4. Ergothioneine and lovastatin content of P. citrinopileatus mushrooms cultivated in three substrates, wheat straw (WS), grape marc (GM), and olive by-products (OL) by using UV-Vis and LC-MS.

Method
Ergothioneine It's interesting to mention that UV-Vis results showed no statistically significant differences (p-value > 0.05) in both ergothioneine and lovastatin contents among mushrooms from three substrates. On the other hand, LC-MS methods indicated that P. citrinopileatus produced in OL contained the highest amount of ergothioneine (although differences were not significant versus those of mushrooms deriving from WS), while P. citrinopileatus cultivated in GM exhibited the highest concentration of lovastatin (Table 4). These results revealed that the nature of growth substrate can play an important role in ergothioneine and lovastatin biosynthesis of P. citrinopileatus. It was observed that ergothioneine and lovastatin levels detected in GM substrates in a polyphenol-rich matrix differed significantly from those determined in WS and OL (Table 4). This is likely due to suppression (ergothioneine) or overexpression (lovastatin) mechanisms involved in the pertinent biosynthetic pathways, resulting in low ergothioneine and high lovastatin content in GM-cultivated mushrooms, respectively.
However, it is possible that these mechanisms may also be associated with various bioactive compounds present in the substrates examined. These compounds can act either as precursors, inducers, or inhibitors of the examined analytes biosynthesis affecting ergothioneine and lovastatin final content in mushrooms [33]. The differences in the type and nature of phenolic compounds and amino acids contained in each one of the studied agricultural by-products may be considered as the key factor for the under-production or over-production of the investigated compounds. However, this is not underlined evidently in existing literature, and further research is required in order to shed light on the effect of substrates' bioactive compounds in the production of ergothioneine and lovastatin. Apart from that, these additional bioactive compounds (i.e., polyphenols, amino acids, etc.) of the growth medium can be absorbed by the mushrooms, increasing not only their nutritional but also their added value [34].

Comparing Analytical Approaches for the Identification and Quantification of Ergothioneine and Lovastatin in Mushrooms 2.4.1. Ultraviolet-visibleSpectroscopy (UV-Vis)
As already mentioned, several bioactive compounds absorb in the wavelengths analyzed for ergothioneine and lovastatin, increasing the possibility of providing false positive errors in the final measurements. More specifically, ergothioneine was analyzed at 260 nm ( Figure 1), which is a region of the spectrum in which some nucleic acids and aromatic amino acids (present in mushrooms) absorb light [35,36]. It is known that nucleic acids represent large amounts of nonprotein nitrogen in fungi [30]. In addition, Pleurotus mushrooms and grape marc by-products contain a higher amount of the aromatic amino acid phenylalanine, which may absorb at the same wavelength used for ergothioneine's spectrophotometric determination, leading to an erroneously increased ergothioneine's peak area (Tables 3 and 4). effect of substrates' bioactive compounds in the production of ergothioneine and lovastatin. Apart from that, these additional bioactive compounds (i.e., polyphenols, amino acids, etc.) of the growth medium can be absorbed by the mushrooms, increasing not only their nutritional but also their added value [34].

Ultraviolet-visibleSpectroscopy (UV-Vis)
As already mentioned, several bioactive compounds absorb in the wavelengths analyzed for ergothioneine and lovastatin, increasing the possibility of providing false positive errors in the final measurements. More specifically, ergothioneine was analyzed at 260 nm (Figure 1), which is a region of the spectrum in which some nucleic acids and aromatic amino acids (present in mushrooms) absorb light [35,36]. It is known that nucleic acids represent large amounts of nonprotein nitrogen in fungi [30]. In addition, Pleurotus mushrooms and grape marc by-products contain a higher amount of the aromatic amino acid phenylalanine, which may absorb at the same wavelength used for ergothioneine's spectrophotometric determination, leading to an erroneously increased ergothioneine's peak area (Tables 3 and 4). Ergothioneine ultra-violet spectroscopy (UV-Vis) spectra of mushrooms of three species, i.e., A. bisporus, P. ostreatus, and P. citrinopileatus. The latter was cultivated in three substrates (wheat straw (WS), grape marc (GM), and olive by-products (OL)).
In contrast, Agaricus mushrooms and olive by-products do not contain equally high concentrations of such amino acids [37,38]. This may be related to the lower ergothioneine content measured in A. bisporus and P. citrinopileatus-OL mushrooms by UV-Vis spectroscopy (Tables 3 and 4). In addition, phenolic content in mushrooms or in their cultivation substrates can affect UV-Vis quantification. These positive errors may affect more P. citrinopileatus samples rather than other mushrooms produced on WS-based substrates, since P. citrinopileatus samples are also derived from agricultural by-products that contain significant amounts of phenolic compounds [39,40]. Especially, wines and wineries residues, like grape marc, contain quercetin rhamnoside, kaempferol, hydroxybenzoic acid deriva- Figure 1. Ergothioneine ultra-violet spectroscopy (UV-Vis) spectra of mushrooms of three species, i.e., A. bisporus, P. ostreatus, and P. citrinopileatus. The latter was cultivated in three substrates (wheat straw (WS), grape marc (GM), and olive byproducts (OL)).
In contrast, Agaricus mushrooms and olive by-products do not contain equally high concentrations of such amino acids [37,38]. This may be related to the lower ergothioneine content measured in A. bisporus and P. citrinopileatus-OL mushrooms by UV-Vis spectroscopy (Tables 3 and 4). In addition, phenolic content in mushrooms or in their cultivation substrates can affect UV-Vis quantification. These positive errors may affect more P. citrinopileatus samples rather than other mushrooms produced on WS-based substrates, since P. citrinopileatus samples are also derived from agricultural by-products that contain significant amounts of phenolic compounds [39,40]. Especially, wines and wineries residues, like grape marc, contain quercetin rhamnoside, kaempferol, hydroxybenzoic acid derivatives, and myricetin 3-O-glucoside that can absorb in similar wavelengths to that of ergothioneine's UV-Vis determination, explaining the excessively higher concentrations of ergothioneine presented in Table 5 [41,42].
Regarding the levels of lovastatin detected, it should be noted that there are some intermediates of the lovastatin biosynthesis pathway and some structural analogues of lovastatin that can absorb light at 232-238 nm, i.e., the wavelengths selected for lovastatin's UV-Vis analysis [43]. These intermediates are mostly degraded compounds, such as methyl esters, anhydro, methoxy, and acetate ester forms of lovastatin, that can absorb in those wavelengths due to the diene groups they include [44]. Similarly, olive by-products (olive leaves and olive mill waste) contain tyrosol, hydroxytyrosol, apigenin, and p-coumaric acid hexoside that can absorb in similar wavelengths to lovastatin [45].
These hypotheses could be supported by the results of the present work (Tables 3 and 4). Moreover, by comparing the UV-Vis spectra of pure lovastatin from literature [46] with the corresponding UV-Vis spectra of the mushroom samples examined (Figure 2), it was noticed that the hydroxyl metabolite of lovastatin likely coexists with the lactone form. This can be assumed by the presence of one broad peak instead of two separate sharp peaks, with one for lovastatin and one for lovastatin acid, which is a highly unstable metabolite that can easily be converted to the lactone form. As proven by the interpretation of the results of UV-Vis analysis, a more selective technique, like LC-MS, may be more suitable for the quantification of ergothioneine and lovastatin in order to avoid a possible interference. ergothioneine presented in Table 5 [41,42].
Regarding the levels of lovastatin detected, it should be noted that there are some intermediates of the lovastatin biosynthesis pathway and some structural analogues of lovastatin that can absorb light at 232-238 nm, i.e., the wavelengths selected for lovastatin's UV-Vis analysis [43]. These intermediates are mostly degraded compounds, such as methyl esters, anhydro, methoxy, and acetate ester forms of lovastatin, that can absorb in those wavelengths due to the diene groups they include [44]. Similarly, olive by-products (olive leaves and olive mill waste) contain tyrosol, hydroxytyrosol, apigenin, and pcoumaric acid hexoside that can absorb in similar wavelengths to lovastatin [45].
These hypotheses could be supported by the results of the present work (Tables 3 and  4). Moreover, by comparing the UV-Vis spectra of pure lovastatin from literature [46] with the corresponding UV-Vis spectra of the mushroom samples examined (Figure 2), it was noticed that the hydroxyl metabolite of lovastatin likely coexists with the lactone form. This can be assumed by the presence of one broad peak instead of two separate sharp peaks, with one for lovastatin and one for lovastatin acid, which is a highly unstable metabolite that can easily be converted to the lactone form. As proven by the interpretation of the results of UV-Vis analysis, a more selective technique, like LC-MS, may be more suitable for the quantification of ergothioneine and lovastatin in order to avoid a possible interference.

Liquid Chromatography-Mass Spectrometry (LC-MS)
Ergothioneine contents determined in P. ostreatus and P. citrinopileatus-WS (Table 3), by the developed LC-MS method, were similar to those reported in other studies, such as Lin et al. [47], who found a 997 mg ergothioneine kg −1 dry sample by using a high-pressure liquid chromatography with a diode array detector (HPLC-DAD) method. Additionally, the LC-MS results of our study were in accordance with results provided by Weigand-Heller et al. [48], who also implemented an LC-MS methodology to evaluate ergothioneine's content in Agaricus mushrooms.

Liquid Chromatography-Mass Spectrometry (LC-MS)
Ergothioneine contents determined in P. ostreatus and P. citrinopileatus-WS (Table 3), by the developed LC-MS method, were similar to those reported in other studies, such as Lin et al. [47], who found a 997 mg ergothioneine kg −1 dry sample by using a high-pressure liquid chromatography with a diode array detector (HPLC-DAD) method. Additionally, the LC-MS results of our study were in accordance with results provided by Weigand-Heller et al. [48], who also implemented an LC-MS methodology to evaluate ergothioneine's content in Agaricus mushrooms.
P. citrinopileatus samples, which were produced in OL, demonstrated the highest ergothioneine content than any other mushroom sample, irrespectively of species or a cultivation substrate, while P. citrinopileatus-GM along with A. bisporus mushrooms contained the lowest content (Tables 3 and 4). As already stated, this finding is possibly related to the different compounds that non-conventional growth substrates contain including phenolic compounds and amino acids. Both amino acids and polyphenols hindered the UV-Vis determination of the two analytes due to their co-absorbance at specific wavelengths.
Lovastatin contents of the studied mushrooms were considerably lower than that reported in other studies [23,24]. The present outcome seems to be related to the greater sensitivity of the Orbitrap MS instrumentation used for lovastatin determination compared to the mass detectors used in the other studies. Relying on the higher resolution and sensitivity of our validated LC-MS methodology, we can assume that it can quantify or even detect lovastatin in samples of extremely low content.
The need to apply a more sensitive and accurate technique for the evaluation of lovastatin content is also underscored by reviewing the presently published literature.
Although, in the current study, lovastatin was detected in all mushroom samples, there are conflicting reports concerning the content of lovastatin in P. ostreatus and P. citrinopileatus. For example, Lam & Okello [25], Lin et al. [47], and Cohen et al. [49] did not detect lovastatin in P. ostreatus and P. citrinopileatus mushrooms, while Lo et al. [24] determined high concentrations of the same analyte. Along with the applied detection methodology, different cultivation practices (strain type, pH, aeration, temperature, and incubation period) as well as methods of extraction and measurement could also affect lovastatin production and determination.
To sum up, LC-MS platforms emerge as the method-of-choice for the accurate determination of both ergothioneine and lovastatin since they circumvent the interferences and drawbacks of UV-Vis protocols. Taking into consideration the results of the developed LC-MS methods, P. citrinopileatus could be considered an efficient alternative to the most common commercial species (i.e., A. bisporus and P. ostreatus) for the production of ergothioneine-rich mushrooms. Even though P. citrinopileatus would not be selected as the most appropriate species (on the basis of the outcome provided by the single strain examined) for producing mushrooms rich in lovastatin, its cultivation in novel non-conventional substrates, e.g., based on grape marc, can raise the final lovastatin content, highlighting the exploitation potential of such by-products in mushroom cultivation.
All standard stock solutions of lovastatin, simvastatin, and methimidazole were prepared in acetonitrile, while acetonitrile-water 7:3 (% v/v) was used to dissolve the watersoluble ergothioneine. The prepared stock solutions were stored at −18 • C. All solvents were of an LC-MS grade. Acetonitrile was purchased from Sigma Aldrich (St. Louis, MO, USA) and water was from Sharlau (Barcelona, Spain). Methanol was provided by ChemLab (Zadeglem, Belgium), while formic acid was obtained from Fisher Scientific (Hampton, VA, USA).

Biological Material-Mushroom Cultivation
Mushrooms of three species (Fungi and Basidiomycota) were examined in this study. Those of Pleurotus ostreatus and Agaricus bisporus were purchased from a local market, while P. citrinopileatus was cultivated at the Laboratory of General and Agricultural Microbiology, Agricultural University of Athens. Three substrates were used for this purpose, i.e., wheat straw (WS), grape marc plus wheat straw (GM; 1:1, w/w), and two-phase olive mill waste plus olive leaves (OL; 1:1, w/w). Their preparation process, the inoculation of the fungal strain (LGAM 158), and the conditions for mushroom production were previously described [50]. Grape marc was obtained by a winery located in Nemea (Peloponnese). Olive leaves and two-phase olive mill waste were obtained from an olive mill located in Kalamata (Peloponnese), and wheat-straw was kindly provided by Dirfis Mushrooms SA (Euboea).

Sample Preparation
After cultivation, whole mushrooms were collected and frozen to −20 • C for one day and freeze dried in a ModulyoD Freeze Dryer, equipped with a Thermo Savant ValuPump VLP200 (Thermo Electron Corporation, Thermo Fischer, Waltham, MA, USA).
Freeze drying was selected as the optimum drying method since it protects sensitive metabolites and bioactive compounds from degradation during long-term storage. This method removes samples' moisture that may produce undesirable chemical reactions and promote microbial growth [51]. Prior to analyses, dried material was homogenized and powdered in a laboratory mill (Type ZM1, Retch GmbH, Haan, Germany). Dry material and all samples and extracts were kept in airtight packaging bags and vials at −20 • C.

Extraction Procedure
The extraction process applied for the ergothioneine recovery was based on an already published protocol [29], slightly modified with regard to the centrifugation conditions. Ergothioneine was extracted from 100 mg of dried mushroom powder with 10 mL of 1:4 (% v/v) aqueous methanol by vigorous shaking for 20 min in a vortex (Falc Instruments, Bergamo, Italy), which is followed by centrifugation (Centrifuge Z32 HK, Hermle, Wehingen, Germany) at 3650 rcf for 20 min. After centrifugation, 8 mL of the supernatant were placed in the freeze dryer in order to acquire the dry residue of the extracts.
The lovastatin extraction procedure was based on a previously developed extraction process with slight modifications [23]. Lovastatin was extracted from 400 mg dried mushroom powder with 4 mL of acetonitrile followed by vigorous shaking (Falc Instruments, Italy). This was followed for 2 h at 250 rpm. The extract was then centrifuged for 20 min at 3650 rcf. Three mL of the supernatant were evaporated using a nitrogen pump to remove the extraction solvent.

Ultraviolet-Visible Spectroscopy (UV-Vis)
Ultraviolet-Visible (UV-Vis) analysis was conducted by using a dual beam spectrophotometer (UV-1900, Shimadzu Corporation, Kyoto Japan), while scanning from 200 to 400 nm was performed to determine maximum wavelengths. After reviewing the spectra of the two investigated compounds, ergothioneine analysis was performed at 260 nm, while lovastatin analysis took place at 232 nm instead of 238 nm in order to minimize possible interferences (Figure 3).

Liquid Chromatography-Mass Spectrometry (LC-MS)
Liquid chromatography mass spectrometry (LC-MS) analysis was used for the identification and quantification of ergothioneine and lovastatin in mushroom species. The instrumentation of liquid chromatography for both methods included a quaternary pump, an autosampler with a tray oven set at 25 °C (Accela, Thermo Scientific, Waltham, USA), and a guard column. For ergothioneine analysis, a Kromacil C18 column (3.5 μm particle size, 100 × 2.1 mm i.d.) was used at 25 °C, while lovastatin separation was per- The linearity of calibration curves was determined by using standard solutions of the two compounds with concentrations ranging from 1 to 20 µg mL −1 for both ergothioneine and lovastatin. Coefficient factors (R 2 ) were 0.9979 for ergothioneine and 0.9988 for lovastatin, verifying the method's linearity. Two to five (2-5) milligrams of each mushroom extract's dry residue were dissolved in 10 mL of 3:7 (v/v) methanol-water for ergothioneine and in 5 mL of acetonitrile for lovastatin. All spectra were processed by UV Probe software (2.7 version, Shimadzu Corporation, Kyoto, Japan).

Liquid Chromatography-Mass Spectrometry (LC-MS)
Liquid chromatography mass spectrometry (LC-MS) analysis was used for the identification and quantification of ergothioneine and lovastatin in mushroom species. The instrumentation of liquid chromatography for both methods included a quaternary pump, an autosampler with a tray oven set at 25 • C (Accela, Thermo Scientific, Waltham, USA), and a guard column. For ergothioneine analysis, a Kromacil C18 column (3.5 µm particle size, 100 × 2.1 mm i.d.) was used at 25 • C, while lovastatin separation was performed by an Acquity C18 column (1.7 particle size, 100 × 2.1 mm i.d.) at 25 • C. Finally, injection volume for both analyses was set at 10 µL.
Ergothioneine and methimazole (I.S) were separated using a 15-min gradient elution program, which consisted of water with 0.1% formic acid (Solvent A) and acetonitrile (Solvent B) at a steady flow rate of 0.2 mL min −1 . The gradient started with 30% of solvent A, increased to 50% over 10 min of analysis, and, in 15 min, the percentage of solvent A ramped to initial conditions (30%). On the other hand, lovastatin and simvastatin (I.S) were separated using a 10-min isocratic elution program, which consisted of water with 0.1% formic acid (Solvent A, 40%) and acetonitrile (Solvent B, 60%).
For ergothioneine analysis, a 3D quadrupole ion trap LCQ FLEET (Thermo Scientific, USA) mass spectrometer was used, while for lovastatin-where a detector of higher resolution was required-an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific, USA) was utilized. Tandem mass spectrometry MS/MS measurements were performed in a positive mode using an electrospray chemical ionization (ESI) source at mass scan width of 100-350 m/z for ergothioneine and 250-550 m/z for lovastatin, respectively. The mass tolerance window for mass identification of product ions was set at ±5 ppm. Source parameters are fully described in Table 5. All spectra were processed by Xcalibur software (Version 3.0, Thermo Scientific, USA). The identification and quantification of the two analytes under determination was based on the fragmentation of the precursor ions into the respective product ions using a single reaction monitoring (SRM) technique. More specifically, product fragments of ergothioneine with m/z = 186.1 (C8H15N3S) were observed (Figure 4a) at a retention time (RT) of 1.10 min. Based on the MS results and previous published data, these fragments are characteristic of the fragmentation of the precursor ion called ergothioneine [16]. An exception was the identification of methimazole for which only the precursor ion with m/z = 114.9, observed at a retention time of 1.38 min, was used for the analysis (Figure 4b).
1 Similarly, during the identification and quantification of lovastatin, a product ion of m/z = 325.1772 (C 17 H 21 ON 6 ) was observed at a retention time of 5.19 min. Even though lovastatin and simvastatin were fragmented to the same product ion, a sufficient separation was achieved because the second compound was eluted at a different retention time of 7.12 min (Figure 5a). Taking into consideration the standard solutions analyzed and previous published results [52], this ion is characteristic of the fragmentation of the precursor ion of lovastatin and simvastatin (Figure 5b).

Liquid Chromatography-Mass Spectrometry Methods' Validation
Liquid chromatography-mass spectrometry (LC-MS) methods' validation was performed in terms of linearity, accuracy, intra-day (repeatability) and inter-day (reproducibility) precision, extraction recovery, and a matrix effect (ME). Validation runs were conducted on three consecutive days. The linearity was determined using fourteen ergothioneine standards with concentrations ranging from 0.05 to 45 µg mL −1 and ten lovastatin standards with concentrations ranging from 0.001 to 1 µg mL −1 . The concentrations of ergothioneine, recorded in mushrooms, present higher variability and a wider concentration range than lovastatin. Thus, a calibration curve including a more extended range of concentrations were constructed in the case of ergothioneine (n = 14 instead of n = 10, in the case of lovastatin). Due to the wider selected concentration range, more concentration levels (n = 14) of the standard solutions were required to assure the linearity of ergothioneine's calibration curve. Finally, in order to determine the detection (LoD) and quantification limits (LoQ) of the two developed methods, the guidelines of the Official Journal of the European Communities was adopted [53]. For that purpose, 0.01 µg mL −1 of ergothioneine and 0.001 µg mL −1 of the lovastatin standard were used, respectively.
For the estimation of intra-day precision and accuracy, three replicates (n = 3) of low, medium, and high concentrations of quality control (QC) samples were analyzed. More specifically for ergothioneine, QC samples of 5, 25, and 40 µg mL −1 were used, while, for lovastatin, QC samples of 0.005, 0.05, and 0.5 µg mL −1 were determined. The inter-day precision (or intermediate precision) was assessed by analysis of three batches of QC on three different days (n = 3 replicates per day, N = 3 different days for each concentration level). The precision was defined as the relative standard deviation (RSD%) and the accuracy was expressed as a relative error (RE%).
For the determination of the extraction recovery for ergothioneine and lovastatin, three different samples were analyzed: un-spiked mushroom samples (A), spiked mushroom samples with 10 µg mL −1 of ergothioneine, or 0.05 µg mL −1 of lovastatin (B) and standard solutions of these corresponding concentrations (C). The equation below (Equation (1)) was used to define the extraction recoveries.
The matrix effect (ME) estimation was conducted at low, medium, and high concentration levels by comparing the peak areas of each analyte spiked in mushroom samples with those of standard solutions at the same concentration. For that purpose, A. bisporus and P. ostreatus mushroom samples were pooled together while P. citrinopileatus mushroom samples, produced in three substrates, were examined separately. Peak areas of standard solutions were defined as A, whereas the peak areas of samples spiked with analyte were defined as B. The ratio below (Equation (2)) was used to evaluate the matrix effect.

Statistical Analysis
The statistical analysis of the results of the different analytical techniques was performed by one-way analysis of variance (ANOVA). In this study, the basic criterion for statistical significance, at a 95% confidence level, was p-value ≤ 0.05. For the calculation of the p-value, three measurements of the samples were included (n = 3).

Conclusions
Despite the fact that UV-Vis is a relatively inexpensive and rapid method, the outcome of the present work suggests that it should not be acknowledged as the most suitable technique for the identification and quantification of ergothioneine and lovastatin in mushrooms due to numerous restrictions imposed by the different co-existing mushroom constituents absorbing at the same wavelength or close wavelengths. Specifically, the phenolic profile and amino acids of mushrooms and cultivation substrates are considered to be the major factors affecting the accurate quantification of these compounds by spectrometric methods. The impact of these bioactive compounds on UV-Vis-determined ergothioneine and lovastatin content is more pronounced in the comparison of mushrooms from different cultivation substrates (GM and OL) rather than among mushroom species. Therefore, the possible application of this technique, could potentially provide misleading results regarding the selection of the most suitable substrate(s) for an ergothioneine-rich or lovastatin-rich mushroom production. Perhaps, the optimization of the implemented extraction methodologies or the replacement of the existing extraction techniques with more selective procedures resulting at higher product yields, could be an area of future investigation.
In contrast, the LC-MS methods implemented, which combined precision and higher sensitivity, showed significant differences in ergothioneine and lovastatin content (Tables 3 and 4) in comparisons among species and substrates that were not observed during UV-Vis determination. Ergothioneine, although detected in all samples, was sig-nificantly higher in P. citrinopileatus mushrooms (822.1 (±20.6) mg kg −1 dry sample). In contrast, the lovastatin content in A. bisporus (1.39 (±0.014 mg kg −1 dry samples) was higher than in Pleurotus mushrooms. Nonetheless, lovastatin levels could be increased by using suitable/alternative cultivation substrates. In addition, P. citrinopileatus mushrooms produced on OL showed the highest levels of ergothioneine, (884.5 (±20.0) mg kg −1 dry sample), while fruitbodies from GM-based substrates contained the highest amounts of lovastatin (0.218 (±0.014) mg kg −1 dry sample).
Since non-conventional substrates seem to have an impact on the biosynthetic pathways and the final content of the examined compounds, the elucidation of the relationship between substrates' content in other bioactive compounds (e.g., phenolics, amino acids) and ergothioneine or lovastatin yields would be an area of investigation. However, more mushroom strains/species and a wider range of substrates need to be studied to provide solid evidence confirming these assumptions.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.