Quinoline Functionalized Schiff Base Silver (I) Complexes: Interactions with Biomolecules and In Vitro Cytotoxicity, Antioxidant and Antimicrobial Activities

A series of fifteen silver (I) quinoline complexes Q1–Q15 have been synthesized and studied for their biological activities. Q1–Q15 were synthesized from the reactions of quinolinyl Schiff base derivatives L1–L5 (obtained by condensing 2-quinolinecarboxaldehyde with various aniline derivatives) with AgNO3, AgClO4 and AgCF3SO3. Q1–Q15 were characterized by various spectroscopic techniques and the structures of [Ag(L1)2]NO3 Q1, [Ag(L1)2]ClO4 Q6, [Ag(L2)2]ClO4 Q7, [Ag(L2)2]CF3SO3 Q12 and [Ag(L4)2]CF3SO3 Q14 were unequivocally determined by single crystal X-ray diffraction analysis. In vitro antimicrobial tests against Gram-positive and Gram-negative bacteria revealed the influence of structure and anion on the complexes′ moderate to excellent antibacterial activity. In vitro antioxidant activities of the complexes showed their good radical scavenging activity in ferric reducing antioxidant power (FRAP). Complexes with the fluorine substituent or the thiophene or benzothiazole moieties are more potent with IC50 between 0.95 and 2.22 mg/mL than the standard used, ascorbic acid (2.68 mg/mL). The compounds showed a strong binding affinity with calf thymus-DNA via an intercalation mode and protein through a static quenching mechanism. Cytotoxicity activity was examined against three carcinoma cell lines (HELA, MDA-MB231, and SHSY5Y). [Ag(L2)2]ClO4 Q7 with a benzothiazole moiety and [Ag(L4)2]ClO4 Q9 with a methyl substituent had excellent cytotoxicity against HELA cells.


Introduction
Schiff bases are an extensively studied important class of ligands. They are commonly synthesized by the condensation reaction of aromatic aldehydes or ketones with primary amines resulting in an azomethine moiety with at least one aryl group attached to either the carbon or nitrogen atom. The interest in Schiff base ligands is therefore as such due to their ease of synthesis, selectivity to the central metal ion [1], synthesis flexibility [2], structural diversity [3], and their broad biological applications [4].
Quinolines are derivatives of pyridine and are found in many biologically active natural [5,6] and synthetic [7][8][9] compounds. Their Schiff base derivatives have also been identified with various pharmaceutical activities [10,11]. Although some free Schiff base ligands are known to be bioactive, literature has shown that incorporating metal in such N im and N qy atoms and in L2 via N thiazole and N qy atoms. This mode of coordination is consistent with other reported silver(I) complexes of similar ligand [34][35][36]. The pattern of coordination is different in complexes Q1, Q6, and Q11. The integration values of Q1, Q6, and Q11 showed that the complexes adopt a 3-coordinate geometry as is evident from their 1 H-NMR spectra. A significant downfield shift in the azomethine protons and upfield shift in the alpha protons with respect to the N atom in the quinoline ring was observed in comparison with their free ligand resonance. In addition to this, protons on the carbon of aniline in Q1, Q6, and Q11 and a singlet of amine protons at δ 5.04 ppm was seen, which shows that the silver ion coordinated to one ligand L1 (through the N im and N qy atoms) and the N atom of aniline from the fraction of the second ligand resulting in a three-coordinate geometry. In the 1 H-NMR spectrum of Q11, the NH 2 proton is absent which could be attributed to the low concentration of the complex in solution. The tricoordinate observed in Q1, Q6, and Q11 are likely due to the steric repulsions between the two Schiff bases resulting in the hydrolysis of one of the ligands. Further, the electron-withdrawing substituent in Q1, Q6, and Q11 could have influenced this type of coordination because electron-withdrawing substituent is known for their ability to reduce electron density around the metal center and thus affects binding interaction between the metal and the coordination donor [37]. The effects of solvent on the Schiff base hydrolysis can also not be overlooked as this type of Schiff base hydrolysis during coordination has been reported [38,39].
Coordination of L1-L5 to the Ag(I) centers were confirmed by comparing the FT-IR spectroscopy (Table S1). Bands observed between 1613 and 1636 cm −1 associated with the -C=N-bond stretching frequencies shifted to higher frequencies between 1624 and 1684 cm −1 in the spectra of the L4 and L5 upon coordination to Ag(I) while in L1 and L3, the -C=N-band shifted to lower frequencies from 1618-1693 cm −1 to 1613-1624 cm −1 , respectively, on coordination to Ag(I).
In the spectrum of L2 with thiazole moiety, the -C=N-bond shifted from 1452 cm −1 to higher frequency on coordination to Ag(I) in the spectra of Q2, Q7, and Q12 between ca. 1456 and 1457 cm −1 . The absorption bands associated with the C=N quinolinyl ring in the range 1591-1593 cm −1 in the spectra of L1, L2, L4, and L5 shifted to the lower frequency on coordination to 1585-1592 cm −1 . In L3 spectra, the C=N quinolinyl ring at 1588 cm −1 blue-shifted to 1591-1592 cm −1 on coordination in the spectra of Q3, Q8, and Q13. Coordination of L2, L3, L4, and L5 to Ag(I) can thus be proposed to be via either N im or N thiazole and N qui donor atoms [34,40,41], while for L1, coordination was via N im , N qui , and N amine donor atoms.
Molar conductivity of Q1-Q15 was measured in dimethylformamide at 25 • C and the values range between 0.28 and 4.05 × 10 4 S m 2 mol −1 (see Supplementary Materials Table S2), indicating that the complexes act as electrolytes.
Electronic absorption spectra recorded in acetonitrile at room temperature in the UV-Vis region of the free L1-L5 ligands and their corresponding complexes Q1-Q15 are presented in Figure 1. The absorption spectra of L1, L3, and L4 (Figure 1a,c,d) and their respective complexes are similar having two major absorption bands in the UV region between 232-275 nm and 300-340 nm. In L2 and its complexes absorption spectra (Figure 1b), five similar absorption bands in the UV region between 216-289 and 318-350 nm attributed to intraligand (IL) π-π * and n-π * transitions respectively are displayed. L5 and its complexes showed one major absorption spectra between 287 and 291, accompanied by two shoulders between 316 and 337 nm. All the complexes bands can be attributed to intra-ligand n-π *, π-π *, and metal to ligand charge transfer transitions (MLCT) [42,43]. A general slight red-shift in the spectra of L1-L5 complexes was observed, which can be associated with a decrease in energy at the excited states upon coordination to silver(I) [44][45][46]. The steric effects of the various substituents were observed to have influenced the mode of coordination in the structures of L1, L2, and L4 complexes. In the structures of L2 complexes, the involvement of anion in the coordination to the Ag(I) center seems to be influenced by the less steric hindrance of the benzothiazole moiety as seen in complexes Q7 and Q12 structures (Figure 2c,d). These types of coordination by trifluoromethanesulfonate anion have been reported [47]. In L1 complexes, the hydrolytic cleavage of one molecule of the Schiff base ligands could be related to the steric repulsions between the two Schiff bases.

Crystal Structures of Complexes Q1, Q6, Q7, Q12, and Q14
The crystals of Q1, Q6, Q7, Q12, and Q14 were each grown by slow evaporation of toluene diffused into dichloromethane solution of the complexes. The structures of Q1 and Q6 confirmed their solution behavior while that of Q7, Q12, and Q14 are different in solution. The asymmetric units of Q1 and Q6 (Figure 2a,b) had a full molecule of the ligand coordinated to the metal center in a bidentate manner, an amine (from the hydrolytic cleavage of the second ligand molecule) and a molecule of the nitrate (Q1) or perchlorate (Q6), kicked out of the coordination sphere. The coordination to the metal center was via a N atom of the quinoline moiety, an imine N atom and a N atom of amine forming a three-coordinate geometry. The silver(I) centers in Q1 and Q6 fits in Davis et al. 2015 [48] "Extended Y"geometry description (120  Table 1). The asymmetric unit of Q7 consists of a disordered ligand, one silver(I) center with a perchlorate anion with coordination via the quinoline N atom, the imine N atom in a bidentate manner, and to an O atom of the perchlorate anion making half of the complex. The other unsymmetrically related half is generated through an Ag.Ag interaction and in the process the Ag(I) centers adopt a pseudo tetrahedral geometry. The bond angles around the Ag(I) center lie between 72.8 (4) and 105.49 (2) • . This type of coordination has been observed in related silver(I) complexes [46,49]. Q12 has a molecule of the disordered ligand, an Ag(I) metal center and a molecule of the trifluoromethanesulfonate anion in the asymmetric unit. The other half is generated through a center of inversion lying in the center of an eight-member metallacycle in which two trifluoromethanesulfonate anions through two O atoms each bridge two metal centers. While the two sets of O atoms occupy two coordination sites of each Ag(I) center, the other two sites are occupied by two N atoms of the ligand in a bidentate manner. The resulting geometry is distorted pseudo tetrahedral with the bond angles around the Ag(I) center being 99.86 (10), 105.57 (10), 162.01 (10), and 175.69 (9) • . A similar mode of coordination has been reported [50]. The asymmetric unit of Q14 has a molecule of the ligand, an Ag(I) metal center, a trifluoromethanesulfonate molecule and a molecule of water. Coordination in this complex is via a N atom of the quinoline moiety, an imine N atom, an O atom of a trifluoromethanesulfonate, and an O atom of a water molecule forming a pseudo tetrahedral with the bond angles around the Ag(I) center lying between 93.18 (6) and 145.17 (6) • . All the complexes feature two sets of planes described by a five-member metallacycle including the quinoline imine unit (Plane 1) and by substituted phenyl ring for Q1, Q6, and Q14 and by the thiazole moiety in the structures of complexes Q7 and Q12.
The Ag-N im bond distances in Q1, Q6, Q7, and Q12 range between 2.317 (18) and 2.465 (3) Å and are generally longer than the Ag-N qy bond distance between the range 2.240 (16) and 2.346 (3) Å but shorter in Q14 (Table 1). The Ag-O bond distances range from 2.233 (16) and 2.609 Å. The Ag-N and Ag-O bond distances in complexes Q1, Q6, Q7, Q12, and Q14 are within expected ranges and are comparable with those reported in the literature [50,51]. Complexes Q7 has Ag Ag interaction where the bond distance is 3.0563 (11) Å.

In Vitro Antimicrobial Studies
All the ligands and complexes were tested against two Gram-positive bacteria, Methicillinresistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (S. aureus), and four Gram-negative bacteria, Escherichia coli (E. coli), Salmonella typhimurium (S. typhi), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa). The minimum inhibitory concentrations (MIC) values were compared to Ciprofloxacin and the three silver(I) salts as standards ( Table 2). The aim was to investigate the effect of introducing the silver metal to the ligands in the bacteria strains. Q1-Q15 showed better antibacterial activity compared to L1-L5 in general an indication of enhanced activity of the ligands on complexation to Ag(I). The general belief is that complexation of the ligands leads to high lipophilicity which in turn can lead to the rupturing of the cell membranes of the microorganisms [52]. In addition, silver(I) ion is well known for its bactericidal effects, mechanistically acting by obstruction of bacteria cell functions through direct interaction with the bacteria enzymes, DNA and the negatively charged peptidoglycan from the bacterial cell wall. This interrupts cell division and replication and cell breathing leading to cell death through the release of their fluid and electrolyte content into the environment [53]. and L3 against P. aeruginosa and K. pneumoniae with MIC values between 50 and 100 µg/mL, L4 against S. typhi and E. coli and L5 against P. aeruginosa, S. typhi, and K. pneumoniae with MIC values of 100 µg/mL, 50 µg/mL, and 100 µg/mL, respectively. Q7 and Q8 showed the highest antimicrobial activity against all bacteria strains. The ligands of each of Q7 and Q8 possess either the benzothiazole moiety or the p-chloro substituent. Enhanced antimicrobial activity of compounds containing either benzothiazole moiety or the chlorine substituent in the para-position is in accordance with the literature report [55,56] making them targets for drug design. In addition, both Q7 and Q8 have the perchlorate anion. Enhance antimicrobial activity was also observed in other complexes with perchlorate anion against most of the bacteria strains. All the complexes with the exception of Q4, Q6, and Q10, were selectively active against K. pneumoniae (in comparison to the other strains) with MIC values between 0.0125 and 0.4 µg/mL, lower than that of Ciprofloxacin (0.8 µg/mL). The activity of Q7 against S. aureus with an MIC value of 1.6 µg/mL can be singled out as it was better than that of the standard ciprofloxacin, AgNO 3 , AgClO 4 , or AgCF 3 SO 3 . Similarly, Q4, Q6, Q11, Q12, and Q15 with an MIC value below 25 µg/mL signified their better activity than ciprofloxacin against S. aureus. Those of Q1, Q2, Q10, and Q13 have the same MIC value as the standard reference against S. aureus. All the complexes are fairly active against MRSA with only Q6 and Q8 with MIC value 25 µg/mL showing the same activities as ciprofloxacin. Q6 and Q8 have fluorine and chlorine respectively, as their substituent and perchlorate as their counter anion. This revealed the influence of electron-withdrawing functional groups on antimicrobial activity. This type of compound with an electron-withdrawing group has been reported to have excellent antimicrobial activity [57]. Against P. aeruginosa, Q8 having chlorine as its substituent, showed excellent activity higher than the reference standards while Q7 has the same MIC value of 0.2 µg/mL as ciprofloxacin. Q14 and Q15 have similar MIC value as AgClO 4 (0.8 µg/mL), Q5, Q6, Q9, Q10, have MIC values within 1.6-6.25 µg/mL lower than AgNO 3 and AgCF 3 SO 3 while Q2, Q3, Q4, Q12, and Q13 (6.25 µg/mL) have similar MIC value as AgNO 3 . Q6, Q10, Q14, and Q15 having either thiophene moiety, fluorine, methyl substituent in their ligand performed better than the standard against P. aeruginosa, these complexes also have either perchlorate or triflate as their anion.
The MIC of all the complexes except Q15 and AgNO 3 (1000 µg/mL) against S. typhi is within 12.5-100 µg/mL, these values are higher than the ciprofloxacin standard (0.4 µg/mL), only AgClO 4 (0.2 µg/mL) had better activity than the standard. Q5 having thiophene moiety and nitrate ion as counter anion has the same MIC value as AgCF 3 SO 3 (12.5 µg/mL). This shows complexes Q1-Q15 fair activity against S. typhi. The activities of Q5 (with thiophene) and Q8 (with chlorine) against E. coli are the same as the standard (1.6 µg/mL). Complexes Q6 and Q9 (12.5 µg/mL) were half of their salt (25 µg/mL), while Q10 (6.25 µg/mL) was four times lower than the salt against E. coli. The enhanced activity of Q8 with chlorine substituent and Q5 (with thiophene) are 64 and 32 times higher than the standard respectively against K. pneumoniae. All the complexes showed excellent antibacterial activities against K. pneumoniae. Q4 has the same MIC value as the standard (0.8 µg/mL).
In summary, the antimicrobial activities of the complexes exhibited a structural activity relationship because those with the chlorine substituents, benzothiazole, and thiophene moieties and with perchlorate anion displayed a remarkable antimicrobial activity. Enhanced antimicrobial activities of compounds containing similar electron-withdrawing groups [58,59], benzothiazole [60], and thiophene [61] moieties have been reported. Reports on the effects of different counter anions are also known [62].
A comparison bar chart representing each salt complex is shown in Figure 3a-c. Compounds with 1000 µg/mL MIC value and those without antimicrobial activity are omitted from the bar plot for clarity.

In Vitro Antioxidant Studies
Antioxidants are important in protecting the human body against damage by reactive oxygen species (ROS). Accumulation of ROS in the cells may lead to damage of the DNA, RNA, and proteins which subsequently leads to several physiological disorders such as cardiovascular disease, diabetes, rheumatism, atherosclerosis, cancer, aging and cell death [63,64]. The antioxidant activity of some compounds such as dithiocarbamate complexes [65] and pyridine derivatives complexes [66][67][68] have been reported to possess the ability to slow down and stop damage to DNA, RNA, protein substrates and thereby prevent the occurrence of diseases. The free radical scavenging ability of all ligands and complexes in this study were investigated using ferric reducing antioxidant power (FRAP). In antioxidant studies, FRAP assay is often preferred because it is simple, fast, economical, and direct in antioxidant measurement. FRAP assay is based on the electrondonating ability of an antioxidant compound. This involves the reduction of a colorless Fe 3+ tripyridyltriazine complex (Fe 3+ (TPTZ)) to an intense blue-colored Fe 2+ tripyridyltriazine complex (Fe 2+ (TPTZ)) by the electron-donating antioxidants at low pH [69]. The reducing power is to determine the reductive ability of the test compounds. Table 3 summarizes the mean values from three independent experiments of in vitro antioxidant activities of the ligands L1-L5 and complexes Q1-Q15 at different concentrations. The antioxidant activities of the compounds are expressed as 50% inhibitory concentration (IC 50 in mg/mL). Ascorbic acid with an IC 50 value of 2.68 mg/mL was used as a standard for the study. All the compounds tested showed some antioxidant activity. Of the ligands, L2 had the highest activity with an IC 50 value of 2.29 mg/mL even better than that of the standard reference. The complexes and the metal salts had better activities than the ligands on the general. The antioxidant activity of the silver salts was evaluated to study the influence of the metal salts on the ligand s antioxidant activity. Interestingly, the silver salts exhibit significant antioxidant activity comparable to the standard, but the introduction of an organic ligand to a metal ion is known to help with the regulation of metal ion absorption, distribution and metabolism in the biological systems [70,71]. Hence, we focus on the antioxidant activity of complexes Q1-Q15 as an alternative synthetic antioxidant in this study.
Q2, Q4, Q5, Q6, Q7, Q9 Q10, and Q13 had IC 50 values between 0.95 and 2.22 mg/mL ( Table 3) better than that of ascorbic acid (2.68 mg/mL) but not those of the free metal salts. The implication is that the complexes had a good ability to reduce Fe 3+ to Fe 2+ (in FRAP) was good. The common occurrence in these complexes is either a fluorine substituent, thiophene or a benzothiazole moiety. There have been reports of compounds with fluorine substituent (C 0.5 FRAP = 424.5-502.6 µM) with potent antioxidant activities in FRAP [72]. Similarly, other reports have shown significant antioxidant activities of benzothiazole (553.5 mmol Fe 2+ /mmol Compound) [73] and thiophene (4.55 mmol TE/g compound) [74] derivatives. In this study, Q6 with fluorine substituent and perchlorate counter anion had the lowest ferric reducing power. It is assumed that the Ag(I) center in Q6 which is tricoordinate, could be responsible for the enhanced activity. Q10 has the thiophene moiety with a lone pair of electrons on its thiophene. However, despite a fluoro substituent in Q1 and a chloro substituent in Q3, the two complexes had the lowest activities, with IC 50 values of 4.97 and 4.75 mg/mL. The counter anions in both complexes is a nitrate and that could be responsible for their low activities. The bar chart in Figure 4 represent Q1-Q15 free radical scavenging activity by FRAP assay. . FRAP % free radical scavenging vs. Concentration (mg/mL) of complexes Q1-Q15, silver nitrate, silver perchlorate, silver triflate, and Ascorbic Acid. AgN = silver nitrate, AgC = silver perchlorate, AgF = silver triflate and As = Ascorbic acid values represent mean ± standard deviation (n = 3). a-f Different alphabets over the bars for a given concentration for each complex represent significant difference (Tukey s-HSD multiple range post hoc test, p < 0.05).
Summarily, most of the complexes tested had significant antioxidant activity. The antioxidant data obtained for complexes Q1-Q15 clearly showed that complexes with either a fluorine substituent, thiophene or a benzothiazole moiety had good reducing power. This is an evidence of their effective antioxidant activities. Thus, these complexes are potential synthetic antioxidant supplements required to protect the body against free radical damages.

DNA Binding Studies
Deoxyribonucleic acid (DNA) is a molecule possessing genetic information in living cells. It is an important molecular target for anticancer agents because of its involvement in gene expression and protein synthesis, which are fundamental steps in cell division and growth [75]. DNA binding studies are significant when developing novel therapeutic drugs. Their interactions with potential drugs have been associated with the anticarcinogenic activity of such drugs. This makes the determination of the type of interaction between metal complexes and DNA important. In studying DNA interaction with metal complexes, electronic absorption spectroscopy is one of the most convenient techniques usually used to investigate the possible modes of binding. DNA can interact with metal complexes either through the covalent or non-covalent bond. Non-covalent interaction of the DNA with metal complexes is usually via three major modes namely: intercalations between base pairs, binding into the groove of DNA, and electrostatic binding [76], while the covalent interaction involves the replacement of the DNA N atom by the metal complex's labile ligand [77].
In this study, we set to examine the extent to which the ligands and the complexes interact with calf thymus DNA (CT-DNA) by monitoring the hyperchromic and hypochromic effects of the electronic absorption titration. Incremental additions of DNA to the test compounds showed prominent hypochromic shifts with intraligand π-π * bands absorption bands between 249 and 372 nm in the absorption spectra of the ligands and complexes studied. The hypochromism is probably due to the formation of strong stacking interaction between the quinoline planar aromatic chromophore and the base pairs of CT-DNA [27] which is an indication of intercalation binding behavior of the compounds to CT-DNA. Intercalators are molecules that stack perpendicular to the DNA backbone without forming covalent bonds or breaking up the hydrogen bonds between DNA bases. The p-orbital of the compounds could be coupled with the p-orbital of the base pairs, which decreases the transition energy and induces hypochromism.
In complexes Q1-Q15, a hypochromic shift between 6.27 and 80.49% in the absorption bands with slight bathochromic between 1 and 3 nm was observed in all the compounds (Supplementary Materials Figures S6-S19) except for Q1, Q6, and Q11, whose absorption bands are accompanied by a slight hypsochromic shift between 2 and 4 nm as the concentration of the CT-DNA was increased. L1-L5, Q1-Q15 and AgNO 3 , AgClO 4 , and AgCF 3 SO 3 in the absence and presence of CT-DNA are shown in Figures S1-S22 (see Supplementary Materials).
AgNO 3 , AgClO 4 , and AgCF 3 SO 3 were used as the standard in this study and the titration of these metal salts against CT-DNA also showed notable hypochromic shifts of 23.64-50.86% with absorption bands between 263 and 274 nm. The silver salts: A hypochromic shift in the absorption bands with a slight bathochromic shift of~2 nm was observed in the spectra of the metal salts (Supplementary Materials Figures S20-S22).
The intrinsic binding constant of L1-L5, Q1-Q15 and AgNO 3 , AgClO 4 , and AgCF 3 SO 3 were evaluated using the Wolfe-Shimer equation (Equation (5)) by determining the ratio of the slope to the intercept from the plot of [DNA]/(ε a − ε f ) versus [DNA]. The binding affinity of the free ligands and free metal salts are lower than those of complexes Q1-Q15, an indication that the ligands binding affinities were enhanced on complexation of ligands to Ag(I). The binding constant obtained lie between 3.33 × 10 5 and 3.0 × 10 6 M −1 for Q1-Q15, between 7.50 × 10 4 and 1.38 × 10 5 in L1-L5, and between 2.00 × 10 5 and 3.30 × 10 5 for AgNO 3 , AgClO 4 , and AgCF 3 SO 3 (Table 4). Q8 had the highest binding affinity (Figure 5a) with a calculated intrinsic binding constant (K b ) of 3.00 × 10 6 , followed by Q3 and Q7 with 1.57 × 10 6 and 1.5 × 10 6 , respectively. The high binding affinities of the three complexes (Q3, Q7, and Q8) are probably due to the π-electron interactions of the quinolinyl with the base pairs of the DNA [78]. In addition, the presence of electron-withdrawing chlorine atoms in para-position in Q3 and Q8 and the presence of benzothiazole moiety in Q7 probably also contributes to the binding affinity to CT-DNA. High DNA interaction with similar compounds has been reported for complexes with electron withdrawing group and benzothiazole moiety [78][79][80]. This interaction favors the aromatic environment of the CT-DNA base pairs resulting in strong structural perturbations in the DNA molecule. These perturbations increase the distance between the adjacent base pairs [27]. The order of the interaction of complexes Q1-Q15 and their metal salts with DNA are Q8 > Q3 > Q7 > Q11 > Q9 = Q10 > Q12 > Q13 > Q1 = Q4 = Q5 = Q15 > Q6 > Q14 > Q2 > AgClO 4 > AgNO 3 > AgCF 3 SO 3 . The binding mode needs to be proved by further studies, whereas ligands L1-L5 were exempted since their binding affinity to CT-DNA are lower than those of the complexes.

Fluorescence Competitive Displacement Studies
Further studies on binding interactions of Q1-Q15 and the silver salts with CT-DNA were done using fluorescence competitive displacement assay. Although the complexes absorb UV radiation, they do not fluoresce. The fluorescence competitive displacement study is based on the displacement of ethidium bromide from the CT-DNA-EB adduct by the test compounds. Ethidium bromide, a well-known intercalating agent, is a planar cationic dye that fluoresces weakly in phosphate buffer saline solution but emits intensely in the presence of CT-DNA due to the strong intercalation between the DNA double helix base pairs [81]. The intense fluorescence of the ethidium bromide in the presence of CT-DNA can be quenched by introducing another molecule that can displace the bound ethidium bromide. A phenomenon known as fluorescence quenching [80]. The incremental addition of the complexes concentration to ethidium bromide pre-treated with CT-DNA resulted in an appreciable decrease in the ethidium bromide fluorescent intensity at 605 nm (Figure 5b and Figures S23-S39). The gradual decrease in the intensity of the EB pre-treated with CT-DNA upon addition of Q1-Q15 complexes could be as a result of the complexes interacting with DNA via intercalation.
The relative binding interaction of the complexes with CT-DNA was determined by the quenching strength, K sv calculated from the slope of the linear quenching plot of F o /F versus [complex] (Equation (6)). The K sv values for Q1-Q15 were between 2.03 and 3.98 × 10 4 M −1 and those of the silver salts are between 3.01 and 3.31 × 10 4 M −1 ( Table 4). These K sv values indicated the displacement of ethidium bromide by Q1-Q15 and confirmed binding via intercalation mode to CT-DNA. The complexes have high quenching efficiency and a binding affinity to CT-DNA. Complex Q8 (Figure 5b) had the highest binding affinity to CT-DNA which is consistent with the compound with the highest intrinsic K b constant (Figure 5a). The quenching of the fluorescence intensity of EB-CT-DNA adduct by the complexes was further used to determine the binding constant (K bin ) and the number of binding sites (n).
Fluorescence quenching can take place via two mechanisms, namely, static quenching and dynamic quenching. The static quenching involves the collision of the fluorophore and the quencher (complex) in the ground state while the dynamic quenching involves the collision of the fluorophore and the quencher in the excited state. In order to determine the type of quenching taking place, two approaches are commonly used, namely: temperaturedependent approach and the Stern-Volmer plot approach.
The Stern-Volmer plot approach was used based on the fact that the Stern-Volmer plots for Q1-Q15 (Figure 5c), were linear an indication of only one mechanism for the quenching fluorescence, i.e., either dynamic or static. Evaluation of K q using Equation (1) would, therefore, suggest the quenching mechanism through which the complexes quench ethidium bromide emission.
where K sv , K q , and τ o represent Stern-Volmer quenching constant, bimolecular quenching constant, and an average lifetime of the fluorophore in the absence of the quencher, respectively. τ o normally takes about 10 −8 s in biomacromolecules [82]. The values of K q were found to be between 2.03 and 3.98 × 10 12 M −1 S −1 (Table 4), which is greater than the maximum diffusion collision quenching rate constant of various quenchers of biological macromolecules (2 × 10 10 M −1 S −1 ) [82]. These values showed that the quenching by Q1-Q15 was not initiated by a dynamic mechanism but happened via a static quenching mechanism; thus, at the ground state, complexes formed between quenching molecules and fluorescence molecules are stabilized [83]. The double-logarithmic Equation (2) was used in determining the binding constant of complexes Q1-Q15 and CT-DNA.  Table 4), suggesting that all the complexes bind to one reactive site on the CT-DNA. The corresponding results are shown in Table 4. Complex Q10 (Figure 5d) showed the most binding affinity to CT-DNA with a binding constant (K bin ) equal to 9.61 × 10 5 M −1 and the number of binding sites (n) approximately equal to 1.
Based on the spectroscopic studies of the interaction of L1-L5 and complexes Q1-Q15 with CT-DNA, it can be summarized that the test compounds in this study had a strong binding affinity to calf thymus DNA, especially those with either electron-withdrawing substituent or benzothiazole moiety. The binding interaction of the compounds to CT-DNA was via intercalation mode mainly due to the planarity of the chelating quinolinyl ligand and the competitive study with EB shows that the complexes can displace EB from the CT-DNA-EB adduct and compete for the DNA-binding sites with EB, which is usually characteristic of an intercalative interaction of compounds with DNA.

Albumin Binding Studies Using Absorption Spectroscopy Titration
Interaction of potential therapeutic drugs with carrier proteins is very important in bio-inorganic studies because the binding properties of the drug-protein reveal the absorption, transportation, distribution, and metabolism of the drugs. For this reason, the protein-binding study of Q1 to Q15 is an important way of studying the compounds metabolism in vivo.
Bovine serum albumin (BSA) was used as the protein standard in this study because of its close resemblance to human serum albumin (HSA), its stability, high solubility, neutrality in many biochemical reactions and low cost [84]. In protein binding study, absorption spectroscopy titration is a known method used in studying the conformational changes in proteins, and this protein is characterized by an intense absorbance peak at 280 nm depicting the microenvironment around tyrosine and tryptophan residue polarity [85]. On each addition of various concentrations of complexes Q1-Q15 and the silver salts to a constant BSA concentration, the intensity of the BSA absorbance intensified between 260 and 300 nm with slight shifts (Figures S57-S73). This shows some binding interactions between the complexes and BSA exist.  (Table 4) which are in conformation with reported related BSA binding constants, normally between 10 4 and 10 6 M −1 [86]. This range is a recognized range for drug-carrier complexes. The silver salts with K b value between 4.46 and 4.82 × 10 4 is lower than those of the complexes except Q1 ( Figure S57) and Q13 ( Figure S68). This shows that the binding affinity of the ligands to BSA was enhanced on coordination. Complex Q12 had the highest binding affinity (Figure 6a), followed by Q4 ( Figure S60) and Q15 ( Figure S70). The influence of Q4, Q12, and Q15 ligand substituents on their binding affinity to BSA can be related to similar literature reports [87][88][89]. The order of binding constant for the complexes is: Q12 > Q15 > Q4 > Q8 > Q7 > Q11 > Q10 > Q2 > Q14 > Q5 > Q6 > Q9 > Q3 > Q1 > Q13.

Fluorescence Quenching of Bovine Serum Albumin in the Presence of Complexes Q1-Q15
The fluorescence quenching of protein can provide information on protein binding mechanisms, binding mode, binding sites and the rates of interaction with drugs or complexes of study [86]. When BSA is excited at 280 nm, it fluoresces strongly between 300 and 400 nm leading to excitation in tyrosine (Tyr) and tryptophan (Trp) residues in proteins. In the presence of a small molecule, BSA has the possibility of undergoing different types of interactions such as hydrophobic, hydrogen bonding, van der Waals, and electrostatic interactions. The ability of a complex to quench BSA fluorescence intensity depends on the hydrophobic interaction between the Trp and the hydrophobic ligand of a complex. The interaction is either enhanced or stabilized by the surrounding amino acid residues [90]. The binding of BSA to compounds was studied by fluorescence measurements at room temperature.
The free BSA has strong fluorescence emission around 350-360 nm when excited at 280 nm and on adding complexes Q1-Q15, the BSA fluorescence intensity of only Q1, Q6, and Q11 decreased gradually as the concentration of these three complexes was increased (Figure 6b and Figures S74-S75). The reduction in the fluorescence intensity of Q1, Q6, and Q11 is accompanied by a redshift of the emission wavelength between 2 and 15 nm, an indication that the interaction of Q1, Q6, and Q11 with BSA causes a conformational change in the protein structure [91]. Furthermore, an isosbestic point between 366 and 388 nm in the spectra of the complex-BSA interaction was observed, implying the formation of a stable complex-protein complex. Q11 (Figure 6b) quenched the intrinsic fluorescence of BSA more than Q1 and Q6.
The inability of all the complexes (with the exception of Q1, Q6, and Q11) to quench the intrinsic fluorescence of BSA after the addition of between 1 and 2 µM concentration could be structurally related because from our previous report where pyridine moiety was involved as against the quinoline moiety in this study, a contrary behavior regarding the interaction of the protein with complexes of similar substituents and moieties were reported. Complexes Q1, Q6, and Q11 mode of coordination are different from other complexes mode of coordination which means their interaction with BSA was probably via the aniline substituent while the quinoline ring of L2, L3, L4, and L5 complexes were not planar enough and are not readily available for quenching the active site of the protein buried in the hydrophobic environment. This suggests no interaction of the complexes with the BSA protein. The quenching mechanism of the Q1, Q6, and Q11 was evaluated using the Stern-Volmer Equation (Equation (7)). K sv values for the complexes were obtained from the slope of the plot of I 0 /I vs.
[Q] and found to be between 2.50 × 10 4 and 4.09 × 10 4 M −1 . The calculated values of K sv and K q for the interaction of the complexes with BSA are given in Table 4. The linearity of the Stern-Volmer plots (inset Figures S64-S71) is an indicator of either a static or a dynamic quenching mechanism. The K q values obtained for the complexes are between 2.50 × 10 12 and 4.09 × 10 12 M −1 S −1, which are greater than the maximum scatter collision-quenching constant of biomacromolecules (2 × 10 10 M −1 S −1 ). This indicates that the interactions between the complexes and BSA are static. Therefore, the binding constant (K bin ) and the number of binding sites (n) can be determined using Scatchard Equation (3): The n and K bin can be calculated from the slope and the intercept of the double logarithm regression curve of log(I o − I)/I versus log[Q] (Figures S76-S77). The values of the binding constant (K bin ) suggest BSA has a moderate binding affinity to the complexes. Complex Q6 (Figure 6c) has the highest binding affinity to BSA. The number of the binding site (n) for Q1, Q6, and Q11 are approximately one; this suggests that one molecule of the complexes bound per bovine serum albumin. The binding constant values of Q1, Q6, and Q11 fall within 10 4 -10 6 M −1, which is the recognized values of non-covalent interaction of BSA with drugs [92].
In summary, the interaction of the complexes with BSA causes conformation changes of both tyrosine and tryptophan environments due to the hydrophobic interactions of the complexes' ligand with BSA. In essence, the complexes possess a moderate binding affinity for protein. Thus, most of the complexes can be stored in protein and can be easily released in desired target areas.

Anticancer Studies
Cancer is one of the major life-threatening diseases worldwide. The clinical application of chemotherapy is still considered a major method in treating cancer, therefore, the development of novel effective anticancer drugs is of importance. For this study, we picked complexes Q6, Q7, and Q9 based on the significant effect the fluorine and methyl substituent and benzothiazole moiety has on their complexes biological activities. All these complexes have perchlorate as their anion since perchlorate has been identified to likely enhance biological activities as seen in the antimicrobial, antioxidant, activities, and DNA and protein binding assays.
The in vitro cytotoxicity of metal complexes, Q6, Q7, Q9, and cisplatin toward human embryonic kidney 293 (HEK293), cervical cancer cell line (HELA), breast cancer cell line (MDA-MB231) and neuronal cancer cell line (SHSY5Y) were examined via standard method. The EC 50 and the selectivity index (SI) values obtained are summarized in Table 5. The result obtained indicate that there is a structure activity relationship of the complexes in terms of cytotoxicity of the complexes. Q7 and Q9 showed potent cytotoxicity activity towards HELA cells with EC 50 values of 22.80 ± 3.11 and 22.34 ± 4.86 µM, respectively which is lower than cisplatin (EC 50 > 50). Q7 and Q9 also showed slight cytotoxicity against MDA-MB231 and SHSY5Y. The remarkable cytotoxicity recorded for Q7 and Q9 towards HELA cells might be due to the presence of benzothiazole moiety and methyl substituent, respectively. In similar reports, Saeed et al. 2010 [93] in their anticancer study of various benzothiazole derivatives obtained IC 50 values in the range 38-46 µM, and Sreelatha et al. 2014 [94], identified the influence of methyl group in the anticancer activity of naphthoquinone amide derivatives against HELA with IC 50 value of 20 µM.
The positive charge of the metal is also known to influence the cytotoxic activity of the complexes. The charge increases the acidity of the coordinated ligand that accept protons [95]. Q6 had low cytotoxicity activity (EC 50 > 100 µM) towards all the cancer cell lines which could be attributed to their unusual mode of coordination. In comparison, the cytotoxic activity of the complexes toward HeLa cell follows the order Q9 > Q7 > Q6.
In All the complexes are less toxic toward the normal cell (HEK293), with EC 50 ≥ 100 µM and are significantly higher than that of cisplatin with EC 50 values of 14.2 ± 3.8 µM. The SI data shown in Table 5 demonstrates the differential activity of the complexes and cisplatin, the greater the SI value is, the more selective it is. An SI value of less than two indicates the general toxicity of the compound. Based on this, the SI values indicate that complexes Q7 and Q9 displayed excellent selectivity towards all the cancer cell lines, while complexes Q6 had better selectivity towards all the cancer cell lines better than the Cisplatin standard.
In conclusion, the cytotoxicity activity of the studied complexes is coordination geometry, structural substituent, and moiety dependent. The introduction of benzothiazole moiety and methyl substituent on the phenyl ring enhance the anticancer activity especially towards HELA, as a consequence, complexes Q7 and Q9 are promising anti-cervical cancer agents. Notably, all the tested complexes are benign to the normal cell and they all demonstrated a remarkable selectivity for cancer cells over normal cells. 1 H NMR and 13 C NMR spectra were recorded on a BRUKER 400 MHz spectrometer in DMSO-d6 and acetone-d6. Chemical shift values are reported in parts per million (ppm) relative to the solvent residual peaks in DMSO-d6 and acetone-d6; 2.5 and 2.05 ppm respectively for 1 H NMR and 39.5 and 29.4 ppm respectively for 13 C NMR. The splitting patterns in 1 H NMR spectra are reported as s for singlet, d for doublet, m for multiplet while J (the coupling constant is given in Hertz). The infrared spectra were recorded using a PerkinElmer Spectrum 100 FT-IR spectrometer, and the data are reported as percentage transmittances at the respective wavenumbers (cm −1 ), between 4000 and 650 cm −1 . The mass spectra were recorded using Shimadzu LCMS-2020 instrument with only molecular ions (M + ) and major fragmentation peaks being reported with intensities quoted as percentages of the base peak. Elemental analyses were performed on Thermal-Scientific Flash 2000 CHNS/O analyzer. All melting points were determined using the Stuart Scientific melting point apparatus. Absorption studies of CT-DNA and protein were recorded using Shimadzu UV-1800 UV-Vis Spectrophotometer. The photoluminescence studies of CT-DNA and protein were recorded using PerkinElmer LS 45 Fluorescence spectrometer.

Synthesis of Quinolinyl Schiff Bases L1-L5
L1-L5 were synthesized using a modified method from the literature [96,97] for similar ligands. The ligands were synthesized by the addition of a solution of 2-quinolinecarboxaldehyde (1 mmol, 0.16 g) in anhydrous ethanol (10 mL) to a solution of 2-fluoroaniline (1 mmol, 0.10 mL), 2-aminothiophenol (1 mmol, 0.10 mL), 4-chloroaniline (1 mmol, 0.13 g), p-toluidine (1 mmol, 0.11 g), and 2-thiophenemethylamine (1 mmol, 0.10 mL), respectively, in anhydrous ethanol (10 mL) in the presence of glacial acetic acid. The resulting reactions for L1, L3, and L4 were refluxed at ca. 80 • C for 4 h while L2 and L5 were subjected to constant stirring at ambient temperature for 6 h. L2, L3, and L4 solutions were evaporated to one-third of their initial volume under reduced pressure after which 10 mL of ether was added to each solutions to afford precipitates. The precipitates were isolated via filtration, washed twice with 10 mL cold ether, recrystallized from ethanol and dried over anhydrous magnesium sulfate. L1 and L5 on the other hand were evaporated completely under pressure. The oil obtained was recrystallized from ethanol and dried over anhydrous magnesium sulfate.

Single-Crystal X-ray Analysis
Crystal evaluation and data collection of Q1, Q6, Q7, Q12, and Q14 were recorded on a Bruker Apex Duo diffractometer equipped with an Oxford Instruments Cryojet operating at 100 (2) K and an Incoatec microsource operating at 30 W power. The data were collected with Mo Kα (λ = 0.71073 Å) radiation at a crystal-to-detector distance of 50 mm using omega and phi scans. The data were reduced with the programme SAINT [98] using outlier rejection, scan speed scaling, as well as standard Lorentz and polarization correction factors. A SADABS [99] semi-empirical multi-scan absorption correction was applied to the data.
The structures of Q1, Q6, Q7, Q12, and Q14 were solved by the direct method using the SHELXS [100] program and refined. The visual crystal structure information was performed using ORTEP-3 [101], system software. Non-hydrogen atoms were first refined isotropically and then by anisotropic refinement with a full-matrix least-squares method based on F 2 using SHELXL [102]. All hydrogen atoms were positioned geometrically, allowed to ride on their parent atoms, and refined isotropically. The crystallographic data and structure refinement parameters for Q1, Q6, Q7, Q12, and Q14 are given in Table S3 (See Supplementary Materials).
In complex Q7, the perchlorate ion including the bidentate ligand was disordered over two positions with each component having 50% site occupancy. The bridging ligand was disordered over a special position with an equal site occupancy factor. PART-1 instruction was used to model the disorder.

Synthesis of Q1-Q15
Q1-Q15 were synthesized by dropwise addition of ethanolic solution (ca. 15 mL) of each of L1-L5 (1 mmol) to an ethanolic (ca. 10 mL) solution of silver nitrate (0.5 mmol, ca. 0.09 g), silver perchlorate (0.5 mmol, ca. 0.10 g), and silver trifluoromethanesulfonate (0.5 mmol, ca. 0.13 g), respectively, under constant stirring in the dark. The reactions were carried out under nitrogen at ambient temperature for 6 h. The resulting precipitates were isolated using a vacuum filter. Afterward, the precipitates were washed with cold ethanol (10 mL × 2) followed by cold ether (10 mL × 2) and dried in vacuo. The obtained complexes were recrystallized by dissolving the complexes in dichloromethane and layering with toluene.

Müeller-Hinton Agar Test Plates Preparation
Sterilized Nutrient agar medium was first prepared by dissolving 38 g of Müeller-Hinton agar (MHA) (Biolab, Midrand, Africa) in distilled water (1 L). The resulting Nutrient agar medium was subjected to sterilization by autoclaving for 15 min at 121 • C and then cooled down to 45 • C in a water bath. The cooled Agar medium was poured in Petri dishes while ensuring a uniform 4 mm depth of the medium and cooling further to ambient temperature [25].

Inoculation Procedure
Complexes Q1-Q15 and their ligands were tested against four Gram-negative bacteria: Salmonella typhimurium ATCC 14026, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 31488; and two Gram-positive bacteria, viz., Staphylococcus aureus ATCC 700699 (methicillin-resistant) and Staphylococcus aureus ATCC 25923. These bacteria were inoculated in a sterilized Nutrient Broth (NB) (Biolab, Midrand, Africa) through streak plate technique and incubated at 37 • C for 18 h. The nutrient broth was sterilized by dissolving 1.3 g in distilled water (100 mL). Ca. 10 mL of the nutrient broth was transferred into a cotton wool-plugged test tubes and wrapped with an aluminium foil. The nutrient broth in the test tubes was then autoclaved for 15 min at 121 • C and cooled to 37 • C. Following this was the isolation of a single colony of the bacteria and inoculated into a 10 mL sterile NB which was then incubated in a shaking incubator at 37 • C for 18 h. Each of the bacteria strain concentrations was adjusted with sterile distilled water to get a final concentration of 1.5 × 10 8 cfu/mL, i.e., 0.5 Mc Farland's Standard using a densitometer (Mc Farland Latvia) [62]. The bacteria were lawn inoculated on the set MHA Petri dishes using a sterile cotton swab. Screening of L1-L5 and Q1-Q15 for antibacterial activity was first carried out by spotting 5 µL of their solution (prepared from the dissolution of 1000 µg of the L1-L5 and Q1-Q15 in 1 mL dimethyl sulfoxide (DMSO)) on an MHA petri dish and incubated at 37 • C for 18 h. The antibacterial activity was determined by looking out for a clear zone at the spotting point. The compounds with potential antimicrobial activity were then tested for their minimum inhibitory concentration (MIC) against six bacterial. Ten serial dilution of L1-L5 and Q1-Q15 was done to obtain 1000 µg/mL to 0.2 µg/mL concentrations, where the compounds showed lower MICs than 0.2 µg/mL, the solution was further diluted 5 times to obtain 0.100 µg/mL to 0.00625 µg/mL concentrations. Evaluation of the compounds MIC was determined by spotting 5 µL of each concentration of L1-L5 and Q1-Q15 on the MHA plates, and the plates were incubated at 37 • C for 18 h. These procedures were done in triplicate to give the accurate lowest concentration of the compounds where no visible bacterial growth was seen after incubation. Ciprofloxacin was used as a standard and DMSO used as a negative control showed no bactericidal effect against all the bacteria strains [50,62,103].

Antioxidant Assay
Antioxidant activity studies of L1-L5 and Q1-Q15 were done using ferric reducing antioxidant power (FRAP) assay. The ferric reducing antioxidant power assay is often used to evaluate the ability of an antioxidant to donate an electron. The reducing ability of the compounds was measured using a previously published method [104] after slight modifications where different concentrations (0.25 mg/mL, 0.5 mg/mL, 1 mg/mL, and 2 mg/mL) of L1-L5 and Q1-Q15 and standard (Ascorbic acid) were mixed with 2.5 mL of phosphate buffer (0.2 mol/L, pH 6.6) and 2.5 mL of 1% w/v potassium ferricyanide respectively. The resulting mixture was incubated at 50 • C for 20 min, and 2.5 mL of 10% trichloroacetic acid was added to acidify the mixture. After that, 1 mL of the acidified mixture was added to 1 mL of distilled water and 0.5 mL of 0.1% FeCl 3 . The absorbance of the resulting solution was then measured at 700 nm. The antioxidant power of the compounds was expressed as a percentage of ferric reducing antioxidant power Ascorbic acid equivalent, as shown in Equation (4).

DNA Binding Experiments
The interaction of the L1-L5 and complexes Q1-Q15 with calf thymus-DNA was carried out in a Phosphate buffer saline solution pH 7.2 stored at 4 • C. The DNA stock solution was prepared by dissolving CT-DNA sodium salt in Phosphate buffer saline solution with continuous stirring overnight. It was filtered and stored at 4 • C and used within four days. The final concentration of CT-DNA sodium salt was determined by UV-visible absorption using the extinction coefficient ε260 = 6600 M −1 cm −1 [105]. The ratio of CT-DNA at 260 and 280 nm UV absorbance was 1.8-1.93:1, indicating that it is pure and sufficiently free of protein contamination. A fixed amount of the L1-L5 and complexes Q1-Q15 (50 µM) were prepared by dissolving an appropriate amount in DMSO. At a low DMSO concentration, the stability of biomolecules is known to be retained [106,107]. Therefore, in all the experimental of the interaction of L1-L5 and complexes Q1-Q15 with biomolecules, the final concentration of DMSO in the incubation mixture was~2% (v/v). Thus, the effect of DMSO on the stability of the biomolecules are negligible.

DNA Absorption Spectral Study
The binding modes of L1-L5 and complexes Q1-Q15 to CT-DNA were characterized through electronic absorption titration by varying concentrations of CT-DNA (0-30 µM) in phosphate buffer saline solution against fixed concentrations (20 µM) of L1-L5 and complexes Q1-Q15. The compounds-CT-DNA mixture was incubated for 10 min before measuring the absorbance using UV-vis absorption spectroscopy [108]. The absorbance of CT-DNA is cancelled by adding equivalent amounts of CT-DNA to both the tested compounds and the reference solutions.
The compounds intrinsic binding constant, K b were determined using Wolfe-Shimer equation as follows: [DNA] where [DNA], ε a , ε b , and ε f are DNA concentration, apparent, fully bound complex, and free complex extinction coefficients, respectively. The ratio of the slope to the intercept from the plot of [DNA]/(ε a − ε f ) against [DNA] gave the binding constant K b of the compounds.

Luminescence Competitive Displacement Study
Ethidium bromide (EB) is one of the most sensitive fluorescent probes which can bind to DNA through intercalation. Competitive binding of complexes Q1-Q15 to DNA with EB could provide information with regards to the DNA-binding affinity.
A competitive displacement study was carried out using fluorescence spectroscopy between CT-DNA pre-treated with ethidium bromide (EB) and complexes Q1-Q15 to establish the complexes actual mode of binding to CT-DNA. Ethidium bromide in buffer solution has low fluorescence intensity due to fluorescence quenching of the free ethidium bromide by the solvent molecule, but on binding through intercalation to DNA, its emission intensity is drastically enhanced [109]. The competitive luminescence displacement assay was done using a modified method [91]. Pre-treated 15 µM solution of EB with 15 µM of CT-DNA was prepared in a phosphate buffer of pH 7.2 and left to equilibrate for 30 min. Keeping CT-DNA-EB concentrations constant, different concentrations (0-20 µM) of the complexes were added at 8 min incubation interval at room temperature. The fluorescence quenching ability of CT-DNA bound EB by the complexes were recorded in the wavelength range of 530-700 nm with an excitation wavelength at 525 nm at 25 • C.
A Stern-Volmer (Equation (6)) was used to evaluate the CT-DNA-EB fluorescence quenching of the complexes. Where F o and F denote the relative fluorescence intensities of CT-DNA in the absence and presence of the quencher, K sv , Stern-Volmer quenching constant, [Q], quencher concentration, Kq, bimolecular quenching constant and τ o , average lifetime of the fluorophore in the absence of the quencher which is typically equal to 10 −8 s in biomacromolecules.

Albumin Binding Assay Using Absorption Spectroscopy Titration
The absorbance assay of albumin binding is a very simple method used in identifying the conformational changes in protein. The studies aimed at the binding of bioactive compounds with protein to provide useful information on their biodistribution, toxicity, and mechanism of action [18]. A stock solution of bovine serum albumin (BSA) was prepared by dissolving an appropriate amount of BSA in phosphate buffer saline solution (pH 7.2) under constant stirring for 1 h at 25 • C. It was kept at 4 • C and used within four days. The BSA concentration was determined spectrophotometrically by using ε280 = 44300 M −1 cm −1 absorption coefficient. The stock solution of the complex was prepared by dissolving 1 mmol of the complexes in DMSO. The absorption titration assay was done by adding different concentrations (0-10 µM) of the complexes to a constant BSA concentration (6 µM). The sample solution was incubated after each addition of the concentrations for 10 min at 25 • C before recording the absorbance at λmax 280 nm. Binding constant K b was calculated from the intercept to the slope ratio of 1/[A-A • ] vs. 1/[complex] linear curve where A and A • represent BSA absorbance in the presence and absence of complexes, respectively [86].

Albumin Binding Studies Using Fluorescence Quenching Method
A solution of 1 µM of BSA was titrated against various concentrations of the complexes (0-35 µM) and incubated for 8 min at 25 • C. BSA was excited at 280 nm and emitted at 346 nm wavelength [92]. K sv constant was determined from the Stern-Volmer equation: where I o and I represent the intensities of the fluorescence in the presence and absence of the quencher respectively while K q , K sv , τ o , [Q] represent the BSA quenching rate constant, dynamic quenching constant, the average lifetime of BSA without the quencher (τ o = 10 −8 s) and concentration of quencher, respectively.

Cytotoxicity Evaluation
In vitro cell viability with cultured cells is a method commonly used for cytotoxicity activities of potential anticancer compounds due to reduced cost speed, and potential for automation, and tests using human cells other than some in vivo animal tests [110]. The significant effect the perchlorate anion has on the complexes' biological activities, as seen in the antimicrobial, antioxidant, DNA and protein binding assays prompted the choice of complexes to be assayed for cytotoxicity activity. Therefore, the cytotoxicity of some selected complexes; Q6, Q7, Q9, and cisplatin as reference standard was evaluated on four human cell lines, human embryonic kidney 293 (HEK293), cervical cancer cell line (HELA), breast cancer cell line (MDA-MB231), and neuronal cancer cell line (SHSY5Y).
The cytotoxicity assay was conducted as per the standard method and as described by Abrahams et al. 2018 [111]. Briefly, 96-well microtiter plates were seeded with HEK293 (non -cancerous cell line), HELA, MDA-MB231, and SHSY5Y at a concentration of 1 × 10 5 cells/mL and allowed to stabilize for 4 h at 37 • C and 5% CO 2 . Thereafter, Q6, Q7, Q9, and cisplatin were added to the plate through twofold serial dilution to allow for eight final compound concentrations ranging from 100 to 0.781 µM in a total volume of 200 µL / well. The plate was then incubated for 72 h at 37 • C and 5% CO 2 . To each well, 20 µL CellTiter 96 AQ ueous One Solution (Promega, Madison, WI, USA) was added and the plates were incubated for 4 h as previously described. Thereafter, absorbance was read at 490 nm on a multiplate reader (Molecular Devices, San Jose, CA, USA). EC 50 values were determined as the concentration of each compound required to reduce cell viability by 50% and were calculated using GraphPad Prism 8 (2019) (GraphPad Software, San Diego, CA, USA). The values recorded are averages of at least three separate experiments (n = 3).

Conclusions
Herein, we have described the synthesis and characterization of silver(I) complexes of five quinolinyl imine. The influence of the quinolinyl imine variable substituents and silver(I) anion on the interaction of the compounds with Ct-DNA, protein, and their antimicrobial, antioxidant, and cytotoxicity activities were evaluated. The antimicrobial evaluation data revealed that all the complexes had moderate to excellent antibacterial activity and complexes [Ag(L2) 2 ]ClO 4 Q7 and [Ag(L3) 2 ]ClO 4 Q8 showed remarkable antimicrobial activity compare to the standard ciprofloxacin against all the bacteria tested. The ligands of each of [Ag(L2) 2 ]ClO 4 Q7 and [Ag(L3) 2 ]ClO 4 Q8 possess either the benzothiazole moiety or the p-chloro substituent, and both complexes have perchlorate as the anion, which could have enhanced their antimicrobial activity. Similarly, complexes with notable antimicrobial activity also displayed good antioxidant activity than the ascorbic acid (2.68 mg/mL) as seen in Q2, Q4, Q5, Q6, Q7, Q9, Q10, and Q13 with IC 50 between 0.95 and 2.22 mg/mL. In the absorption and emission spectral of the interaction of the compounds with CT-DNA and bovine serum albumin, the results indicate that the compounds bind to CT-DNA via intercalation mode due to the planarity of the chelating ligand and the competitive study with ethidium bromide shows that the complexes can displace EB from the CT-DNA-EB adduct and compete for the DNA-binding sites with EB, which is usually characteristic of the intercalative interaction of compounds with DNA.
Further study demonstrated that the complexes exhibit a moderate affinity for protein and the possible quenching mechanism between the complexes and bovine serum albumin is static. Thus, [Ag(L1) 2 ]NO 3 Q1, [Ag(L1) 2 ]ClO 4 Q6 and [Ag(L1) 2 ]CF 3 SO 3 Q11 can be stored in protein and can be easily released in desired target areas.