Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics

Cosmetic industries are highly committed to finding natural sources of functional active constituents preferable to safer materials to meet consumers’ demands. Marine macroalgae have diversified bioactive constituents and possess potential benefits in beauty care products. Hence, the present study was carried out to characterize the biochemical profile of marine macroalga Chaetomorpha crassa by using different techniques for revealing its cosmetic potentials. In results, the FTIR study characterized the presence of different bioactive functional groups that are responsible for many skin-beneficial compounds whereas six and fifteen different important phycocompounds were found in GCMS analysis of ethanolic and methanolic extracts, respectively. In the saccharide profile of C. crassa, a total of eight different carbohydrate derivatives were determined by the HRLCMS Q-TOF technique, which showed wide varieties of cosmetic interest. In ICP AES analysis, Si was found to be highest whereas Cu was found to be lowest among other elements. A total of twenty-one amino acids were measured by the HRLCMS-QTOF technique, which revealed the highest amount of the amino acid, Aspartic acid (1207.45 nmol/mL) and tyrosine (106.77 nmol/mL) was found to be the lowest in amount among other amino acids. Their cosmetic potentials have been studied based on previous research studies. The incorporation of seaweed-based bioactive components in cosmetics has been extensively growing due to its skin health-promoting effects.


Introduction
Cosmetic products are a topical combination of cosmetic and pharmaceutical in cosmeceutical with biologically active ingredients to have medicinal or drug-like benefits to improve skin health [1]. Due to the modern lifestyle and increasing beauty concerns, the beauty care industries are growing each year all over the world. To meet the demand of consumers, these industries are moving towards the unstoppable use of synthetic cosmetics and ingredients. Due to the ineffectiveness of synthetic ingredients, they may accumulate in skin layers and create toxicities and harm to normal skin health. According to Kerdudo et al. [2], hydroxybenzoic acid esters (parabens), which are widely used in cosmetic formulations, were reported as harmful to the skin as well as causing an increased incidence of breast cancer and malignant melanoma. Phthalates, as an example, are commonly found in several cosmetic products that can cause DNA modifications and damage as proved in human sperm cells [3]. Some of these chemicals can cause harmful effects in animal studies such as decreased sperm counts, altered pregnancy outcomes, male genitalia congenital disabilities, etc. [4]. As a result, people have changed their preferences and opted for natural cosmetic products. Hence, the ever-expanding market for skincare products and continual search for an alternative natural ingredient has led to the development of a multitude of skin cosmetic formulations [5].
Seaweeds, also known as macroalgae are eukaryotic, multicellular, macroscopic, marine photosynthetic eukaryotic organisms that are ubiquitously distributed along with all kinds of coasts from tropical regions to polar regions. It is normally inhabiting the intertidal and sub-tidal regions of coastal areas [6]. Macroalgae are mainly classified into three major types, namely Brown algae (Phaeophyta phylum, Phaeophyceae class), red algae (Rhodophyta phylum, Rhodophyceae class), and green algae (Chlorophyta phylum, Chlorophyceae class). In which, green and red algae belong to the Plantae kingdom whereas brown algae belong to the Chromista kingdom [7]. Marine macroalgae are a rich source of structurally diverse biological active constituents. Algae contain ten times greater diversified phycocompounds than terrestrial plants. The biologically active constituents found in marine macroalgae have multiple activities, which allow them to be used as an active constituent in formulations. The utilization of macroalgae in cosmetic applications is based on their valuable bioactive compounds, such as carbohydrates, proteins, amino acids, lipids, fatty acids, phenolic compounds, pigments, vitamins, and minerals [8] Broad applications of marine macroalgae are based on valuable bioactive compounds and potential bioactivities. For the development of cosmetic formulations, seaweed-derived compounds have been given considerable importance.
C. crassa is a hair-shaped green alga, similarly known as spaghetti algae. It is rapidgrowing, hardy, and possesses a bright green to greenish-white appearance. It mainly absorbs nitrate and phosphate for its growth. The thalli of this algae constitute unbranched filaments forming clumps resembling a ball of fishing line. Filaments of C. crassa consist of cylindrical cells about 600 to 700 µ in diameter. It is very commonly found in various sea sites on the Western Coast of Gujarat, India. It has maximum growth in an intertidal (supralittoral) zone of the beyt Dwarka sea coast due to the availability of moderate temperature in the growing season from November to February. There is no previous reporting on an application of C. crassa in any cosmetic formulation or application. This alga can make a new benchmark in the cosmetic sector after successful experimentation and clinical studies. Besides, this study will be helpful to know the cosmetic potential of C. crassa and become helpful to lead this alga from sea to beauty market after its biochemical profiling.
Many previous research studies demonstrated the wide range of biological activities of seaweed-derived active molecules that can offer a variety of skin benefits. The present study aims to characterize green alga Chaetomorpha crassa derived bioactive compounds, polysaccharides, fatty acids, amino acids, minerals by using different characterization techniques and to reveal its cosmetic potentials by previous research studies.

Collection of Chaetomorpha crassa
The fresh algal sample was collected in polythene bags containing seawater from the coastal line of Beyt Dwarka (22 • 28 43.5 N 69 • 08 08.8 E), Western coast of Gujarat, India, and transported (10 • C) to the laboratory. Sample collection sites were illustrated in Figure 1. The identification of the collected sample was carried out with the help of Dr. Nilesh H. Joshi, at the Fisheries Department, Junagadh Agriculture University, Okha. Identified image of C. crassa is revealed in Figure 2. The sample was collected by handpicking from the intertidal zone during low tide conditions (Precipitation: 0%, Humidity: 55%, Wind: 11 km/h, 27 • C, February 2021). Then the algal material was washed thoroughly to remove extra epiphytes, salts, and marine debris in running tap water. Finally, it was washed with distilled water and allowed to shed dry (room temperature) for five to seven days, until the moisture was completely removed. The dried material was ground to a fine powder using an electric grinder and stored in air-tight containers at −20 • C temperature until further analysis. and transported (10 °C) to the laboratory. Sample collection sites were illustrated in Figure  1. The identification of the collected sample was carried out with the help of Dr. Nilesh H. Joshi, at the Fisheries Department, Junagadh Agriculture University, Okha. Identified image of C. crassa is revealed in Figure 2. The sample was collected by handpicking from the intertidal zone during low tide conditions (Precipitation: 0%, Humidity: 55%, Wind: 11 km/h, 27 °C, February 2021). Then the algal material was washed thoroughly to remove extra epiphytes, salts, and marine debris in running tap water. Finally, it was washed with distilled water and allowed to shed dry (room temperature) for five to seven days, until the moisture was completely removed. The dried material was ground to a fine powder using an electric grinder and stored in air-tight containers at −20 °C temperature until further analysis.  and transported (10 °C) to the laboratory. Sample collection sites were illustrated in Figure  1. The identification of the collected sample was carried out with the help of Dr. Nilesh H. Joshi, at the Fisheries Department, Junagadh Agriculture University, Okha. Identified image of C. crassa is revealed in Figure 2. The sample was collected by handpicking from the intertidal zone during low tide conditions (Precipitation: 0%, Humidity: 55%, Wind: 11 km/h, 27 °C, February 2021). Then the algal material was washed thoroughly to remove extra epiphytes, salts, and marine debris in running tap water. Finally, it was washed with distilled water and allowed to shed dry (room temperature) for five to seven days, until the moisture was completely removed. The dried material was ground to a fine powder using an electric grinder and stored in air-tight containers at −20 °C temperature until further analysis.

Extract Preparation
The extraction was carried out by using 500 g of dry powder in ethanol (80%) at 70 • C by continuous hot percolation using the Soxhlet apparatus. It was continued for 24 h, and the ethanolic extract was then filtered and kept in a hot air oven (RDHO 80, Dry hot air oven, REMI, Bengaluru, India) at 40 • C for 24 h to evaporate the ethanol (Sigma-Aldrich, Bengaluru, India) from it. The extract obtained was concentrated to dryness under decreased pressure (150 mbar) at 20 • C using a rotary vacuum evaporator (Sigma Scientific, Nanganallur, Chennai, India). The obtained residue was kept separately in airtight containers and stored in a deep freezer (−20 • C, Esquire Biotech, Chennai, India).

GCMS Characterization
For GC-MS analysis, the sample was injected into an EB-5 column in the JMS-T100GCV GC model used for chromatographic separation. Helium was used as carrier gas with a flow rate of 1 mL/min; the injector was operated at 200 • C and column oven temperature was programmed as 50-250 • C at a rate of 10 • C/min injection mode. The following MS conditions were used: For mass spectrometry analysis, AccuTof Mass from jeol was used. ionization voltage of 70 eV; ion source temperature of 250 • C; interface temperature of 250 • C; the mass range of 50-600 mass units. A gas chromatogram was obtained and the mass spectrum of the unknown phycocompounds was compared with the spectrum of the known components stored in the NIST library version 2005.

Fatty Acids Characterization by GCMS Analysis
The prepared fine powder of algal material was extracted with methanol (anhydrous, 99.8%, Sigma-Aldrich, Bengaluru, India) in the ratio of 1:10 (w/v) in a flask for 72 h. The mixture was filtered in a separate container. This process was repeated two times with the same residues using a fresh solvent. After the collection of supernatants, the excess solvent was removed by a rotary evaporator (Sigma Scientific, Nangainallur, Chennai, India) and followed for further analysis. The GC-MS analysis was carried out using a JMS-T100GCV gas chromatograph used and coupled to a mass detector AccuTof Mass from jeol. For analysis, the sample was injected into an HP-5 column. The specifications include ion source temperature of 250 • C; interface temperature of 250 • C; mass range of 50-600 mass units and helium flow rate as one mL/min. The ionization voltage was 70 eV. The samples were injected in split mode as 1:10. A gas chromatogram was obtained and the mass spectrum of each unknown compound was interpreted with the spectrum of the known compounds stored in the NIST (National Institute Standard and Technology) library version 2005. The name, molecular weight, and structure of unknown compounds were retrieved. The relative percentage amounts of each compound were calculated by comparing its average peak area to the total area.

Characterization of Functional Groups by FTIR Study
FTIR is the most powerful tool for identifying the types of functional groups present in compounds. The wavelength of light absorbed is characteristic of the chemical bond as can be seen in the spectrum. In analysis, 3000 Hyperion Microscope with Vertex 80 FTIR System, Bruker, Germany. A total of 5 mg of dried powder was taken, KBr (Potassium bromide) (FT-IR grade, ≥99% trace metal basis, Sigma-Aldrich, India) was added and evenly mixed with the sample to obtain a homogenized fine powder. The prepared powder was then placed in the molds and pressed using mechanical support for half a minute using a sterile spatula. The prepared pellet of the sample with the molds was shifted on the pan and was proceeded for analysis. The scanning was performed at wavelength 400-4000 cm −1 , The scanning results were displayed in % transmission analysis. The functional groups of the components were separated based on their peak ratio (based on the peak values in the region of IR radiation).

Chemical Preparation
Standard pure grade reagents (Merck, Germany) were used for the above digestion procedure throughout the experiment. Other solvents such as nitric acid, hydrochloric acid, hydrogen peroxide, and hydrogen fluoride were of analytical reagent grade (Merck). All glassware as well as plastic wares were soaked with 10% nitric acid (ACS reagent, Sigma-Aldrich, India) overnight and then rinsed with distilled water before use.

Microwave-Assisted Digestion and Detection
Approximately 0.05 g of sample was weighed directly into the TFM-PTFE (secondgeneration modified Polytetra-fluoro-ethylene-) vessels, to which the reagents were added (3 mL Hydrochloric acid + 1 mL Nitric acid + 1 mL Hydrogen fluoride + 1 mL Hydrogen peroxide) (Sigma-Aldrich, India) and the vessels were closed immediately. The operational conditions and the heating program were carried out after subjecting the above mixture to a microwave digestor (Titan Microwave system, PerkinElmer, Thane, India) according to the conditions: 130 degrees ramp time 15 min hold time 10 min, and 190 degrees ramp time 15 min hold time 10 min. After cooling these vessels at 70 • C, they were vented and opened. Then, made up to 25 mL by adding Milli-Q water into the vessels and then shaken thoroughly to dilute any possible rest of the colloids attached to the vessels' walls. Blank was also subjected to microwave digestion. The Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES) instrument model used was ARCOS, Simultaneous ICP Spectrometer from SPECTRO Analytical Instruments GmbH, Germany. The software used was Smart Analyzer Vision 5.01.0921. The detector was a charge-coupled device (CCD). All the samples were analyzed in triplicate.

Determination of Total Amino Acid Profile by HRLCMS-QTOF
Acid Hydrolysis Procedure 100 mg sample was weighed into a test tube. Add 12 mL of 6 N HCl in it and the tubes were heat sealed after filling pure nitrogen gas. Keep the test tube in Hot air oven (RDHO 80, Dry hot air oven, REMI, India) at 120 • C temperature for 16 h for hydrolysis. After hydrolysis, the content was removed and filtered. Flash Evaporation was carried out to remove traces of HCl. The residue was made up to a definite volume with 0.05 N HCl. It was filtered again through a Whatman membrane filter of 0.45-micron size. The sample was injected into 6550 iFunnel Q-TOFs, Agilent Technologies, USA. The column used is Poroshell HPH C18-4.6 × 100 mm, 2.7-micron. The oven temperature was maintained at 60 • C and analysis was carried out with the non-switching flow method and fluorescence detection after post-column derivatization. The Standard of amino acids was also run to calculate the concentration of the amino acid depending on the standard chromatogram.

Acid Hydrolysis
The sample was first dried in an oven at 40 • C for 48 h, then powdered to obtain approximately uniform fine particles. A total of 100 mg of the sample was placed in screw-cap tubes (16 mm 25 mm) and add 10 mL of 2 M hydrochloric acid containing 1% phenol. Then, the tubes were closed under nitrogen gas and kept in an electric oven (REMI, Maharashtra, India) at 80 • C for 3 h, allowing it to cool, and their contents were vacuum-filtered through Whatman no. 41 paper. After filtration, the filtrate was diluted to 25 mL with ultrapure water in a volumetric flask and the resulting liquid was again membrane-filtered to get the hydrolysate.

HRLCMS Q-TOF Analysis
Polysaccharides present in the extract of Chaetomorpha crassa were identified using an advanced analytical technique, HRLCMS QTOF. The test sample was analyzed by HRLCMS Q-TOF analysis (6550 iFunnel Q-TOFs, Agilent Technologies, USA) and characterized by setting its scanning range from 150-1000 m/z for MS/MS Dual AJS ESI in the mode of ionization. The Hypersil GOLD C18 Column, size 100 mm × 2.1 mm × 3 µm was used.

Phycocompounds Characterization by GC-MS Analysis
The GCMS analysis of ethanolic extract showed six compounds that were identified based on retention time, % peak area, molecular formula, and molecular weight. A gas chromatogram with different peaks is represented in Figure 3. A total of six different compounds were detected from the ethanolic extract of Chaetomorpha crassa (Table 1).
with ultrapure water in a volumetric flask and the resulting liquid was again membranefiltered to get the hydrolysate.

HRLCMS Q-TOF Analysis
Polysaccharides present in the extract of Chaetomorpha crassa were identified using an advanced analytical technique, HRLCMS QTOF. The test sample was analyzed by HRLCMS Q-TOF analysis (6550 iFunnel Q-TOFs, Agilent Technologies, USA) and characterized by setting its scanning range from 150-1000 m/z for MS/MS Dual AJS ESI in the mode of ionization. The Hypersil GOLD C18 Column, size 100 mm × 2.1 mm × 3 µm was used. Resolved peaks were further identified with the help of reported values from the literature. Above analysis was performed with the following settings: Source parameters include: Gas

Phycocompounds Characterization by GC-MS Analysis
The GCMS analysis of ethanolic extract showed six compounds that were identified based on retention time, % peak area, molecular formula, and molecular weight. A gas chromatogram with different peaks is represented in Figure 3. A total of six different compounds were detected from the ethanolic extract of Chaetomorpha crassa (Table 1).    Among the identified compounds, Dodecanoic acid,1,2,3-propanetriyl ester is found to be the major compound which revealed the largest peak (48.89%) with the retention time (37.03 min). Chemical structures of obtained compounds are depicted in supplementary data Table S1.

Fatty Derivatives Characterization by GC-MS Analysis
In GC-MS characterization analysis, identification of phycocompounds was carried out based on retention time, peak area, molecular formula, and molecular weight. A gas chromatogram with different peaks is represented in Figure 4. Total fifteen different fatty acids were detected from the methanolic extract of Chaetomorpha crassa ( Among the identified compounds, Dodecanoic acid,1,2,3-propanetriyl ester is found to be the major compound which revealed the largest peak (48.89%) with the retention time (37.03 min). Chemical structures of obtained compounds are depicted in supplementary data Table S1.

Fatty Derivatives Characterization by GC-MS Analysis
In GC-MS characterization analysis, identification of phycocompounds was carried out based on retention time, peak area, molecular formula, and molecular weight. A gas chromatogram with different peaks is represented in Figure 4. Total fifteen different fatty acids were detected from the methanolic extract of Chaetomorpha crassa (Table 2).      Among the identified compounds, 9-Octadecenoic acid [Z]-, phenylmethyl ester is found to be the major compound that attained the largest peak (16.64%) with a retention time (39.1 min) whereas Oxirane, tetradecyl-showed the lowest peak area of 0.85% with a retention time of 26.41 min.

Fourier Transform Infrared Spectrophotometer (FTIR) Analysis
FTIR spectrum analysis was used to characterize the functional group of active constituents based on the peak value in the infrared region. The FTIR spectrum is illustrated in Figure 5. The result of FTIR peak values and functional groups is represented in Table 3 H/C=0/C=N/C-N/N-O/CO-O-CO/C-Cl/C-Br/C=C. This result of FTIR spectroscopic analysis revealed the presence of amine, alcohol, phenol, aromatics, alkanes, alkenes, anhydride, carboxylic acids, ester, sulfoxide, nitro, ether, and substituted constituents in the extract of Chaetomorpha crassa.  Table 3).   Table 3).

Determination of Elements by ICP-AES Analysis
Minerals are metallic elements present in various forms in diversified marine algae. They are highly applicable in skin cosmetics formulations. The macromolecules such as Silicon, Potassium, Calcium, Iron, and Magnesium are among the minerals which are present in significant amounts in selected marine alga C. crassa among screened elements. The result showed that Silicon was the highest in amount whereas Copper is the lowest in amount. The percentage of minerals follow Si > K > Ca > Fe > Mg > Na > Zn > B > Cu order. Concentrations for each element (in %) are given in Table 4.

Determination of Amino Acids
Total 21 different amino acids were analyzed in C. crassa by HRLCMS-QTOF analysis. A gas chromatogram with different peaks for different amino acids is represented in Figure 6. Among all, Aspartic acid, Glutamic acid, Hydroxyproline, Glycine, and Alanine were found dominant in the detection above 0.5 g/kg. Aspartic acid amino acid (1.6 g/kg) was found to be the highest in amount whereas amino acid Tyrosine (0.2 g/kg) remained lowest in the above measurement. Detected amino acids were following below order in content (nmol/mL): Aspartic acid > Glutamic acid > Hydroxyproline > Glycine > Alanine > Serine > Leucine > Valine > Threonine > Lysine > Phenylalanine > Arginine > Isoleucine > Methionine > Tyrosine. Concentrations for each amino acid (in g/kg) are expressed in Table 5. Table 5. Determination of total amino acid profile of C. crassa by using HRLCMS-QTOF.

Sr.no
Amino Acids g/kg

Discussion
Marine macroalgae-derived ingredients have been used in skincare applications due to their potential skin benefits. The European Chemicals Agency reported the role of Hydroperoxide, 1-methylhexyl in cosmetic and personal care products for antimicrobial and antioxidant activity [9]. It is also applicable as dyestuffs during oxidative hair dyeing, Dicarboxylic acids and derivatives, Oxalic acid, isobutyl nonyl ester have been demonstrated as having cosmetic benefits as a pH adjuster, fragrance enhancer, as well as skin conditioning agent-emollient [10]. Dodecanoic acid,1,2,3-propanetriyl ester, also known as Trilaurin, presented its use in skin conditioning agents (for maintaining skin functions) as well as occlusive or viscosity increasing agents [11]. Rajamanikyam et al. [12] studied the antibacterial activity of phthalic acid ester against different bacterial species such as S. epidermidis, S. aureus, P. aeruginosa, Klebsiella pneumoniae, and Bacillus subtilis. In addition, Bakkali et al. [13] suggested the use of phthalic acid ester compound in skin cosmetic applications. Patil et al. [14] suggested the use of alkane class compound Tritriacontane, 13-decyl-13-heptyl-for skin benefits as emollients. Two markedly available products Jarcane™ 16 and Jeechem ® NDA-H reported containing Hexadecane. The former product is useful for waxes, moisturizing agents, softening texturing agents, and solubilizers whereas the second product is used for softening texturing agents, thickeners stabilizers, and emollients.
Fatty acids have an important role in the maintenance of skin functions. First, fatty acids are an important player in normal skin barrier functions. Long-chain fatty acids become helpful in moisture retention, prevent the entry of harmful substances. They also maintain certain metabolic processes of skin cells. They can provide moisturizing and antiinflammatory effects. Likewise, medium-chain fatty acids protect against inflammation and tumor factors. In addition, short-chain fatty acids can provide anti-inflammatory effects and initiate an immune response [15]. Cui et al. [16] suggested the antibacterial and anti-inflammatory properties of fatty acids. In addition, fatty acids have some other skin benefits in moisturization and lubrication of the skin [17].
A previous research study reported the presence of various biologically active constituents such as alkanes, aldehydes, alcohols, ethers, ketones, amides, and carboxylic acids by FTIR spectroscopic study [18]. FTIR has been revealed to be a significant means for the classification and differentiation of intimately relevant species of diverse organisms [19]. Janakiraman et al. [20] showed alkyl halides and alkanes function as groups responsible for antimicrobial activity against Staphylococcus aureus, Candida albicans, and Escherichia coli. The presence of functional groups such as alcohol, aromatic, amine, and carboxylic acids are responsible for the antibacterial activity, which was characterized by FTIR analysis [21]. Likewise, Ambedkar et al. [22] revealed carboxylic acid, alkene, aldehyde, unsaturated ketone, phenol, and halogen groups by FTIR study in ethanolic extract and their antibacterial activity against S. aureus, Bacillus subtilis, E. coli. Alcohol group-containing compounds such as triclosan, chlorobutanol, chlorocresol, phenoxyethanol, dichlorobenzyl alcohol, benzyl alcohol, chlorophene, etc. are useful as preservatives [23]. The European Commission (EC) reported the use of the alcoholic compound triclosan in cosmetic applications mouthwashes, toothpaste, hand soaps, face powder, and body soap [24]. Moreover, the aldehyde group-containing compounds such as formaldehyde are a preservative in shampoos, shower gel, and liquid soaps [25]. Culler et al. [26] found a relationship between alkyl, amide, amines-containing compounds, and antimicrobial activity. In addition, nitrogenous compounds present antibacterial and antifungal activities by a different mode of action [27]. Regarding another nitrogen compound, triclocarban inhibits the growth of Gram-positive bacteria include Methicillin-resistant Staphylococcus aureus and resistant Enterococcus sp. [28]. Another nitrogen compound, piroctone olamine, reduces microbial colonization by inhibiting the growth of Malassezia spp. by showing antifungal activity [29]. e-CFR [30] reported the role of alcoholic and halogenated compounds in skincare products for cleaning and bacterial reduction application as antibacterial soap bars, antibacterial wipes, and disinfectants. Siemer [31], suggested the use of the halogenated compound in face care products for antiseptic cuticle treatment and antiacne as well as showed its effect against Staphylococcus aureus, S. epidermis, and Propionibacterium acnes. Some alcohol and halogen group-containing compounds have useful applications in deodorants and antiperspirants by inhibiting the growth of Staphylococci and Diphtheroids of the Corynebacteriaceae family [32].
Many previous research studies confirmed the beneficial role of minerals for skin health benefits. Elias et al. [33] reported the role of calcium in hemostasis as well as a key regulator of epithelialization, which is critically important for the differentiation of basal keratinocytes to corneocytes. Ca ++ acts as a key modulator in the locomotion of keratinocytes and promotes wound healing activity in vivo. Potassium and Sodium both demonstrated cellular and extracellular electrolytes and osmolytes for maintaining cell membrane potential. Concerning the skin benefits, Magnesium is beneficial for skin nourishment with a solution prepared from Dead Sea salts [34]. Denda et al. [35] revealed the effects of topical applications of Magnesium and Calcium salts in skin repair. Topical treatment with 5% MgCl 2 before UVB radiation reduces the number of Langerhan cells in the epidermis as well as decreases antigen-presenting activity in the skin [36]. Although, Zn appears to be safe and effective in many topical formulations. The FDA of the United States recognizes three zinc compounds, zinc acetate, zinc carbonate, and zinc oxide for safe and effective use as topical skin protectants [37]. Some research studies suggested that the presence of zinc in tissue is important in wound healing activity. The research study demonstrated that zinc oxide can be used in topical sunscreen formulations to some extent due to its safe and effective use for skin rashes as well as eczema, impetigo, ringworm, ulcers, pruritus, and psoriasis [38]. Iron is another important most abundant trace metal with various skin beneficial activities. Mainly, it plays a major role in skinrelevant procollagen-proline dioxygenases [39]. Interestingly, Iron is helpful against UVinduced damage by expressing a higher amount of Iron in UV-exposed skin (53.0 ppm) than unexposed skin (17.8 ppm) [40]. Moreover, Copper plays a role in the enhancement of collagen formation and improves skin health. In beauty products, copper-peptides were proven to relax skin irritation., improve skin elasticity, firmness, repair photodamage, reduce fine lines, and antiwrinkle activity with some other benefits [41].
Previous research studies confirmed that the amino acids demonstrated antiinflammatory, antioxidant, photoprotection, moisturizing, antiwrinkle, antielastase, and skin health benefits [42]. Histidine, Lysine, and Arginine are the three most common amino acids for skincare products as well as Glycine, Proline, and Leucine, usefully abundant in collagen. Likewise, a combination of Lysine and Arginine can effectively treat wound healing, while the combination of Proline and Leucine can repair skin wrinkles [43]. Veis and Anesey [44], reported very important collagen and elastin functions of Lysine. Supplementation of Lysine has been useful for the prevention of acne and cold sores. Moreover, Choi et al. [45] demonstrated the role of Tripeptide containing Lysine and Histidine as a skin moisturizer in a skincare product. Isoleucine, Leucine, Valine plus Arginine, Glycine, and Proline significantly enhanced the synthesis of dermal collagen in hairless mice against UV radiation [46]. A combination of Leucine with Glycine and Proline was also used for the attenuation of skin wrinkles [47]. Kawashima et al. [47] reported that topical treatment with a Proline derivative showed an improvement of skin elasticity in Crow's feet lines on the faces of Japanese women. Serine and Alanine amino acids as moisturizing agents can be used in skincare products. It plays a general role in water retention in the stratum corneum. Yamane et al. [48] suggested the role of leucine and isoleucine on collagen synthesis in the skin. Isoleucine has been also used as a complement component in ceramides-based emollient cream for the treatment of facial atopic eczema [49]. Aromatic amino acids such as Phenylalanine and Tyrosine have an important role in melanin synthesis as a precursor. Melanin is the main skin cutaneous protective pigment because it mainly absorbs the harmful sunlight ultraviolet radiation and prevents DNA damage and skin cancers [50]. In addition, Phenylalanine, as well as Tyrosine, play an important role in melanin synthesis (Melanogenesis) [51]. Methionine can also be used in the treatment of acne and inflammatory cutaneous lesions as antioxidant complexes [52]. Threonine and Serine are useful for keeping hydration in the stratum corneum. Arginine is one of the highly recommended amino acids to enhance the wound healing effect of injured skin [53]. Moreover, Glycine and Proline amino acids are not only present in collagens but are also regulators of collagen synthesis [54].
Antibiotic JI-20A demonstrated antibacterial activity in vitro and in vivo against pathogenic bacterium [55]. Gentamycin C1a also reported antibacterial activity by binding with 30S ribosomal unit, negatively impacting protein synthesis [56]. Loquatoside showed significant antioxidant, anti-inflammatory, antiviral, antibacterial, antifungal, and anticancer effects [57]. Feruloyl C1-glucuronide reported its role as an antioxidant compound [58]. Likewise, antiviral activity was reported by Miglustat (alkylated imino analog of D-glucose) in the previous study [59]. Bactobolin was found to be a broad-spectrum antibiotic agent on different bacterial species [60]. Capryloylglycine has many functional benefits such as antibacterial activity, controller of excessive sebum secretion (antiacne), as well as an antiwrinkle agent. It is also applied as a conditioning agent or as a surfactant to protect the skin from water loss [61]. Sačková and Fedoročko, [62] studied anticancer activities such as antiproliferative, cytotoxic, antimetastatic, and proautophagic whereas non-anticancer activities such as antifungal, antibacterial, anti-inflammatory effects, etc. of Manumycin A. 16-methyl-6Z,9Z,12Z heptadecatrienoic acid reported antioxidant activity in vitro which was studied by Yang et al. [63]. Table 7 represents the different bioactive compounds except for polysaccharides in this analysis with their PubChem ID, molecular formula, molecular weight, retention time, abundance, and hits.  Yang et al. [64] found wound healing benefits as well as immune regulation, antioxidant, antiaging activities of Axisothiocyanate 3. Phytosphingosine enhances moisture levels in the human skin barrier through modulation of the FLG (filaggrin) synthesis pathway and suggested the formation of the Natural Moisturizing factor [65]. Antimicrobial, antiinflammation, and antitumor activities of 4-Hydroxy-3-nitrosobenzamide were found, which may be useful for skin cosmetic formulation [66]. Tetradecylamine, similarly known as Myristamine oxide has been demonstrated as a foam stabilizer and hair conditioning agent in shampoos and conditioners [67]. Additionally, 34a-Deoxy-rifamycin W has been known for antibacterial activity against many pathogenic bacteria. Its derivatives are also active against many Gram-positive and Gram-negative cocci, mycobacteria, and chlamydia [68]. (+/−)-3-[(2-methyl-3furyl)thio]-2-butanone was also reported to have benefits as a flavoring agent and provides a cooling sensation [69]. Antifungal, as well as insecticidal activities, were found from the quinoxaline derivative, Chinomethionat, similarly known as Quinomethionate [70]. Another compound beta-Butoxyethyl nicotinate (Nicoboxil) is useful in cosmetics as an antiaging, antiwrinkle agent and helpful in inhibition of photocarcinogenesis. It was also checked for its anti-inflammatory effects against acne, rosacea, and mustard-induced irritation [71].
There is growing interest and potential towards seaweed-derived molecules in cosmetic formulation due to the skin benefits and applications [72]. However, it is well established that the cosmetic industries became more interested in macroalgae going further for extraction of various active constituents and their utility in cosmetic formulations. Marine algae are a source of new value-added active compounds with scientific evidence revealing their benefits for safer use in cosmetics [73]. Marine algae-derived molecules have wide diverse applications in many skin biological benefits such as anti-inflammatory, antimicrobial, anticellulite, antiviral, anticancer, skin whitening, antiaging, antiwrinkle, photoprotection, moisturizer, collagen-boosting activities, etc. [42,74]. Moreover, many cosmetic companies already use marine algae extracts and derived compounds in their formulations [75]. However, the biochemical profile monitoring of macroalgae presents a problem that industries need to overcome. The development of its cultivation and green extraction procedure presents the major key for this thematic, which is being analyzed with promising benefits [76]. However, the exact mechanisms of some compounds have not been fully explored in performing biological functions. Therefore, further studies are essential to understand the exact mechanisms, as well as more clinical trials, in order to improve the quality of cosmetic products which will be helpful to enhance the consumer's safety.

Conclusions
The current findings revealed that the Chlorophyta C. crassa is a good source of active ingredients with much cosmetic potential. In the findings, some of the long-chain fatty acids, Linoleic acids and derivatives, Ergosterols derivatives, and Stigmastane derivatives were found that have moisturization, skin barrier functions, antibacterial, antifungal, antiinflammation, immunostimulatory benefits to the skin. Likewise, in the HRLCMS-QTOF characterization study, some carbohydrate derivatives such as 2-Methyl-2-butenyl beta-Dglucopyranoside; Antibiotic JI 20A; Feruloyl C1-glucuronide; N-butyl-1-deoxynojirimycin; N-(1-Oxooctyl)glycine and other phycocompounds were screened that possess antioxidant, antiwrinkle, antiacne, moisturizer, antimicrobial, conditioning agent, etc. skincare benefits. Amino acids and minerals are the most powerful, effective, and non-toxic important ingredients for skin protection against UV damage, antioxidant, anti-melanogenesis, skin whitening, antiaging, and collagen synthesis activities. Aspartic acid, Glutamic acid, Hydroxyproline, Glycine, Alanine, and Serine were obtained in larger amounts among other amino acids whereas Si, K, Ca, Fe and Mg elements were found higher in C. crassa as compared to other elements. This study revealed a very good biochemical profile and great cosmetic potential of marine alga C. crassa. These algae and their derived molecules may be useful in skincare applications after successful experimentation. Moreover, there is growing interest and potential towards seaweed-derived molecules in cosmetic formulations due to their skin benefits and applications. Apart from that, phycocompounds from marine algae are more effective when used in cosmetic formulations as they are less harmful as well as natural for the skin structure compared to synthetic compounds.
By different biochemical characterizations, this alga represented a good number of potential ingredients. C. crassa or derived compounds can be used as or as a part of a moisturizer, photoprotection formulation, conditioner, skin whitening products as well as in an anti-wrinkle or soothing cream formulations. Moreover, it provides antimicrobial protection and physical health to the skin due to the presence of potent constituents. Along with its potential large availability, (with no worry about the cultivation of C. crassa in the Beyt Dwarka sea coast), it could easily be used in skincare formulations after successful in vitro evaluations and clinical studies.
Supplementary Materials: The following are available online, Table S1: Chemical structures obtained in Methanolic extract of C. crassa.