Engineered Bacteriorhodopsin May Induce Lung Cancer Cell Cycle Arrest and Suppress Their Proliferation and Migration

Highly expressible bacteriorhodopsin (HEBR) is a light-triggered protein (optogenetic protein) that has seven transmembrane regions with retinal bound as their chromophore to sense light. HEBR has controllable photochemical properties and regulates activity on proton pumping. In this study, we generated HEBR protein and incubated with lung cancer cell lines (A549 and H1299) to evaluate if there was a growth-inhibitory effect with or without light illumination. The data revealed that the HEBR protein suppressed cell proliferation and induced the G0/G1 cell cycle arrest without light illumination. Moreover, the migration abilities of A549 and H1299 cells were reduced by ~17% and ~31% after incubation with HEBR (40 μg/mL) for 4 h. The Snail-1 gene expression level of the A549 cells was significantly downregulated by ~50% after the treatment of HEBR. In addition, HEBR significantly inhibited the gene expression of Sox-2 and Oct-4 in H1299 cells. These results suggested that the HEBR protein may inhibit cell proliferation and cell cycle progression of lung cancer cells, reduce their migration activity, and suppress some stemness-related genes. These findings also suggested the potential of HEBR protein to regulate the growth and migration of tumor cells, which may offer the possibility for an anticancer drug.


Introduction
The Halophilic archaea (haloarchaea) are grown in aerobic heterotrophs that dominate hypersaline environments. Hypersaline conditions, such as salt lakes, salt ponds, and solar saltern facilities, are usually exposed to strong sunlight, which leads to the higher salinity than that of sea water [1,2]. To protect the damage from light energy and high salinity, the haloarchaea generate a proton gradient system through the photo-reactive rhodopsin proteins [3]. The first discovered microbial rhodopsins (M-Rho) are typically seven-pass transmembrane helix region bound with retinal to absorb light energy for ion translocation or phototaxis response [4,5]. The photochemical properties of ion-translocating rhodopsins fall into several categories, such as the light-driven outward proton transport bacteriorhodopsin (BR) [4,6], the light-driven inward chloride transport halorhodopsin (HR) [7,8], outward sodium transport (KR2) [9], and the nonselective cation channel channelrhodopsin II (ChR2) [10,11].
Bacterial rhodopsin (BR), a highly stable light-driven proton pump, can excrete proton out of the cell through light perception and regulate the ion balance of the cell [12,13]. In the early stage, BR protein was mostly produced from the slow-growing extreme halophilic microorganisms and few from genetically recombinant Escherichia coli (E. coli). However, the low availability and high cost of BR protein limited its potential of bioengineering

HEBR Protein Suppresses the Cell Proliferation of A549 and H1299 Cells
Before light exposure to the HEBR-treated cells, we utilized immunofluorescent staining to confirm if the HEBR proteins could be delivered into A549 and H1299 cells, after incubation for 24 h with the culture medium containing 20 µg/mL of HEBR proteins. The data showed that HEBR proteins were obviously distributed on the cell membrane and in the cytoplasm of the stained cells, indicated by red arrows in the magnified images ( Figure 1A). These data indicated that the effect of light stimulation on HEBR-treated cells should be evaluated after 24 h of incubation to ensure that the HEBR protein had entered cells. After light stimulation, the proliferation of the HEBR-treated cells was determined by CCK-8 assay ( Figure 1B). The data showed that the proliferation of A549 and H1299 cells decreased in a dose-dependent manner after treatment of HEBR protein with or without green-light illumination. As shown in Figure 1B, the viability of A549 cells could be reduced below~50% at the concentration of 40 µg/mL without the green-light exposure, while the proliferation of H1299 cells remained above 50% under the same condition. At the same time, a significantly decreased proliferation of H1299 cells was observed at the HEBR concentration of 80 µg/mL. For HEBR-treated cells with green-light illumination, the curve of A549 cells after treatment with HEBR protein was slightly different from those without light exposure. The results revealed no significantly differences between the green-light-illuminated or non-illuminated groups on either A549 or H1299 cells after the treatment of HEBR protein. by CCK-8 assay ( Figure 1B). The data showed that the proliferation of A549 and H1299 cells decreased in a dose-dependent manner after treatment of HEBR protein with or without green-light illumination. As shown in Figure 1B, the viability of A549 cells could be reduced below ~50% at the concentration of 40 μg/mL without the green-light exposure, while the proliferation of H1299 cells remained above 50% under the same condition. At the same time, a significantly decreased proliferation of H1299 cells was observed at the HEBR concentration of 80 μg/mL. For HEBR-treated cells with green-light illumination, the curve of A549 cells after treatment with HEBR protein was slightly different from those without light exposure. The results revealed no significantly differences between the green-light-illuminated or non-illuminated groups on either A549 or H1299 cells after the treatment of HEBR protein.

Cell Cycle Arrest in HEBR-Protein-Treated A549 Cells
To reveal the anti-proliferative mechanism of the HEBR protein, we assessed the effect of HEBR treatment on the cell cycle progression. Since the HEBR proteins had more growth-inhibitory effect on A549 cells than on H1299 cells. The A549 cells were selected for further investigation. A two-step cell cycle assay was carried out to quantify the distribution of DNA contents in different cell cycle stages under the concentration of 20 µg/mL HEBR in combination with (or without) green-light illumination on A549 (Figure 2A). Data showed the cell cycle G 0 /G 1 phase arrest induced by the treatment of HEBR on A549 cells ( Figure 2B). Meanwhile, no apparent differences between the greenlight-illuminated or non-illuminated groups were observed in different cell cycle stages on either HEBR-treated or non-treated cells. The HEBR protein was found to promote high DNA contents at G 0 /G 1 phase of the cell cycle with or without green-light exposure, indicating that A549 cells treated with HEBR protein induced cell cycle arrest at G 0 /G 1 phase. These data suggested that HEBR protein could have growth-inhibitory effects on lung cancer cells without green-light exposure.

Scratch Assay on HEBR-Treated Cells
Migration assay is usually used in discovery of antitumor agents, as the agents that inhibit cell migration ability are potential anti-metastatic drugs. In this study, the scratch test was utilized to measure the migration ability of A549 and H1299 cells after treatment of HEBR protein (20 µg/mL or 40 µg/mL) without green-light illumination ( Figure 3A) for a period of 6 h. The cellular migration of each HEBR-treated cells was quantified as a percentage and represented by bar diagrams at 0 h, 1 h, 2 h, and 4 h ( Figure 3B). The HEBR protein did not reduce the migration ability of A549 cells within 6 h. The A549 cells treated with 40 µg/mL HEBR protein showed slightly decreased cell migration compared to A549 cells treated with 20 µg/mL HEBR protein within the 4 h of observation. HEBR protein at 20 µg/mL demonstrated a low inhibitory effect on the cell migration ability of H1299 and A549. Notably, the migration rate of H1299 cells was reduced to about 20% after HEBR treatment of 40 µg/mL for 4 h compare to un-treated cells ( Figure 3B). The data showed that high concentrations of HEBR protein significantly inhibited the migration of H1299 cells rather than that of A549 cells, and the effect was clearly observed after 4 h of incubation time.

Transwell Assay of HEBR Protein on A549 and H1299 Cells
Since HEBR protein of 40 µg/mL showed a strong inhibitory effect on the migration of H1299 cells in the scratch assay, we further utilized a transwell migration assay to confirm the inhibitory effects on the cell migration of A549 and H1299 after treatment with HEBR (40 µg/mL) for 24 h (Figure 4). The results revealed that HEBR protein reduced the number of each type of lung cancer cells to pass through the transwell membrane compared to the non-treated cells ( Figure 4A). It was evident that after HEBR protein treatment, H1299 cells had a lower number of migrated cells than A549 cells ( Figure 4B). The transwell assay again proved that the dose of 40 µg/mL HEBR had a significantly inhibitory effect on the migration of H1299 cells. minated or non-illuminated groups were observed in different cell cycle stages on either HEBR-treated or non-treated cells. The HEBR protein was found to promote high DNA contents at G0/G1 phase of the cell cycle with or without green-light exposure, indicating that A549 cells treated with HEBR protein induced cell cycle arrest at G0/G1 phase. These data suggested that HEBR protein could have growth-inhibitory effects on lung cancer cells without green-light exposure.

Scratch Assay on HEBR-Treated Cells
Migration assay is usually used in discovery of antitumor agents, as the agents that inhibit cell migration ability are potential anti-metastatic drugs. In this study, the scratch test was utilized to measure the migration ability of A549 and H1299 cells after treatment of HEBR protein (20 μg/mL or 40 μg/mL) without green-light illumination ( Figure 3A) for a period of 6 h. The cellular migration of each HEBR-treated cells was quantified as a

Gene Expression of A549 and H1299 Cells after Treatment with HEBR Protein
The mRNA expression levels of EMT genes (Snail-1, Twist-1, E-cad, and N-cad) and stemness genes (Sox-2, Oct-4, and CD133) for the illuminated or non-illuminated groups of HEBR-treated cells were quantified by qRT-PCR, and the results are shown in Figure 5. The gene expression level of Snail-1 for HEBR-protein-treated A549 cells was decreased either with or without green-light illumination ( Figure 5). In contrast, the Twist-1 expression in A549 cells after treatment of HEBR protein was upregulated when compared with nontreated A549 cells. Meanwhile, HEBR protein did not significantly influence the expression levels of E-cad and N-cad for A549 cells under different conditions of light exposure.
of HEBR-treated cells were quantified by qRT-PCR, and the results are shown in Figure 5. The gene expression level of Snail-1 for HEBR-protein-treated A549 cells was decreased either with or without green-light illumination ( Figure 5). In contrast, the Twist-1 expression in A549 cells after treatment of HEBR protein was upregulated when compared with non-treated A549 cells. Meanwhile, HEBR protein did not significantly influence the expression levels of E-cad and N-cad for A549 cells under different conditions of light exposure. Figure 5. Gene expression of EMT HEBR treatment in A549 and H1299 cells. The cells were treated with HEBR proteins for 72 h with and without green-light illumination. The gene expression levels of markers for EMT, including Snail-1, Twist-1, E-cad, and N-cad, were analyzed for A549 or H1299 cells by qRT-PCR and calculated by GraphPad Prism 5 software. The expression of each marker was normalized to that of GAPDH in each group and then presented as the relative expression level to the mock group. L: treated with green-light illumination. Asterisks indicate statistically significant differences, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Figure 5. Gene expression of EMT HEBR treatment in A549 and H1299 cells. The cells were treated with HEBR proteins for 72 h with and without green-light illumination. The gene expression levels of markers for EMT, including Snail-1, Twist-1, E-cad, and N-cad, were analyzed for A549 or H1299 cells by qRT-PCR and calculated by GraphPad Prism 5 software. The expression of each marker was normalized to that of GAPDH in each group and then presented as the relative expression level to the mock group. L: treated with green-light illumination. Asterisks indicate statistically significant differences, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
The expression of stemness-related genes in A549 and H1299 cells after treatment with HEBR protein with or without green-light exposure is displayed in Figure 6. The expression levels of Oct-4 did not display obvious difference between HEBR-treated and non-treated A549 cells. In addition, A549 cells in the non-treated groups expressed more Sox-2 gene than those in HEBR-treated groups. However, the expression level of CD133 in A549 cells was significantly increased in the HEBR-treated groups than that in the non-treated groups. The results showed that the HEBR protein significantly upregulated the expression of both Sox-2 and Oct-4 genes in H1299 cells, while increased the expression of CD133 ( Figure 6). The expression of these stemness-related genes had no dramatic difference between green-light-illuminated groups and non-illuminated groups for both A549 and H1299 cells. These findings were consistent with the results of cell viability. expression levels of Oct-4 did not display obvious difference between HEBR-treated and non-treated A549 cells. In addition, A549 cells in the non-treated groups expressed more Sox-2 gene than those in HEBR-treated groups. However, the expression level of CD133 in A549 cells was significantly increased in the HEBR-treated groups than that in the nontreated groups. The results showed that the HEBR protein significantly upregulated the expression of both Sox-2 and Oct-4 genes in H1299 cells, while increased the expression of CD133 (Figure 6). The expression of these stemness-related genes had no dramatic difference between green-light-illuminated groups and non-illuminated groups for both A549 and H1299 cells. These findings were consistent with the results of cell viability. Figure 6. Gene expression of stemness after HEBR treatment in A549 and H1299 cells. The cells were treated with HEBR proteins for 72 h with and without green-light illumination. The gene expression levels of stemness markers included Sox-2, Oct-4, and CD133, for A549 and H1299, respectively. The expression of each marker was normalized to that of GAPDH in each group and then presented as the relative expression level to the mock group and analyzed by GraphPad Prism 5 software. L: treated with green-light illumination. Asterisks indicate statistically significant differences, * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001.

Discussion
The HEBR protein is a light-trigger proton pump that has optogenetic effects to regulate the function of ion flow artificially, which has been used to investigate new photomodulable treatments via specific lighting stimulation on neural cells in our previous reports [16,32,33]. Modulation of light-triggered proteins has been reported to develop novel treatments based on light-driven control of neural activity in many optogenetic experiments [34]. For instance, the strategy of optogenetic activation increased the possible effects on serotonin nerve conduction to retrieve lost memory in Alzheimer's disease through proton inflow [35]. Artificial manipulation of neural activity could induce the activation of glutamate receptors through action potentials for improving recognition memory in mouse models [36]. However, a few studies have developed photoactivatable cancer drug based on the function of ion channels under light control [37]. In many recent studies, optical control of intracellular signals was frequently used to design cancer drugs via regulation of signaling pathways [38][39][40]. Therefore, controllable ion channels might be an alternative optogenetic tool to develop antitumor drugs. In the present study, we used different concentrations of HEBR protein to evaluate the effect on lung cancer cells Figure 6. Gene expression of stemness after HEBR treatment in A549 and H1299 cells. The cells were treated with HEBR proteins for 72 h with and without green-light illumination. The gene expression levels of stemness markers included Sox-2, Oct-4, and CD133, for A549 and H1299, respectively. The expression of each marker was normalized to that of GAPDH in each group and then presented as the relative expression level to the mock group and analyzed by GraphPad Prism 5 software. L: treated with green-light illumination. Asterisks indicate statistically significant differences, * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001.

Discussion
The HEBR protein is a light-trigger proton pump that has optogenetic effects to regulate the function of ion flow artificially, which has been used to investigate new photomodulable treatments via specific lighting stimulation on neural cells in our previous reports [16,32,33]. Modulation of light-triggered proteins has been reported to develop novel treatments based on light-driven control of neural activity in many optogenetic experiments [34]. For instance, the strategy of optogenetic activation increased the possible effects on serotonin nerve conduction to retrieve lost memory in Alzheimer's disease through proton inflow [35]. Artificial manipulation of neural activity could induce the activation of glutamate receptors through action potentials for improving recognition memory in mouse models [36]. However, a few studies have developed photoactivatable cancer drug based on the function of ion channels under light control [37]. In many recent studies, optical control of intracellular signals was frequently used to design cancer drugs via regulation of signaling pathways [38][39][40]. Therefore, controllable ion channels might be an alternative optogenetic tool to develop antitumor drugs. In the present study, we used different concentrations of HEBR protein to evaluate the effect on lung cancer cells under different light-illuminated conditions. After treatment with proton ion pump proteins on A549 cells without light illumination, significant effects of growth inhibition on cancer cells by HEBR protein of the concentration of 40 µg/mL were observed. This is different from a previous study which demonstrated that HEBR-plasmid-transfected cells could pump out proton ion after green-light illumination [33]. No dramatic difference effects in the proliferation of A549 and H1299 cells was observed between the green-light group and the no-light group after the application of HEBR proteins. Thus, the effects of light-driven proton-pump activity may not play a major role in reducing the cell growth of the two types of cancer cells examined in this study. In addition, we noticed that the growth of human fibroblasts was not strongly affected by the treatment of HEBR protein under different conditions of light exposure ( Figure S1), indicating that normal cells experienced less cytotoxic effects than the two types of cancer cells upon HEBR treatment.
Since the HEBR protein was more cytotoxic to A549 versus H1299 cells, we further investigated if the cell death of A549 was induced by HEBR-protein treatment under greenlight stimulation. The cell cycle distribution showed that 20 µg/mL HEBR protein could induce cell cycle arrest at G 0 /G 1 stage for A549 cells. However, there was no significant difference between light-illuminated and non-illuminated A549 cells after the treatment of 20 µg/mL HEBR protein for 24 h. This finding was consistent with the proliferation assay of A549 cells after the treatment of HEBR protein under different green-light-exposure conditions. These observations again suggested that the HEBR protein could cause growthinhibitory effects on A549 cells without activation of the proton pump.
The metastasis of brain and bone is the leading cause of mortality in patients with lung cancer [41]. Wound healing and transwell assays are commonly used to quantify cellular migration ability. In the present study, we showed that HEBR effectively inhibited the migration of A549 and H1299 cells (Figures 3 and 4). Besides, the epithelial-mesenchymal transition (EMT) program is a variable and dynamic multi-step process that occurs prior to tumor cell metastasis. Cancer cell metastasis relies on the migratory abilities of mesenchymal cells, which allow their entry into the surrounding blood vessels and lymph channels for distant metastasis [42]. In this study, HEBR significantly inhibited the migration ability of A549 and H1299 cells (Figures 3 and 4), which suggested that HEBR may prevent cell metastasis by inhibiting the EMT program of lung cancer cells. When the EMT process starts, Snail is upregulated and E-cad is downregulated, both of which promote the shift of epithelial cells to a mesenchymal phenotype to increase tumor metastasis ability [43]. The HEBR treatment significantly upregulated the Twist-1 gene expression levels but downregulated the Snail-1 gene expression levels of A549 cells, which led to the decrease in cell migration ability. Meanwhile, the N-cad gene expression levels were downregulated, but the Snail-1 gene expression levels were upregulated in H1299 cells after the treatment of HEBR. These results suggested that HEBR might inhibit the EMT of A549 and H1299 cells by a different signaling pathway.
Indeed, the application of HEBR significantly reduced Sox-2 gene expression in A549 and H1299 cells. Many reports have suggested that SOX-2 plays an important role in cancer and cancer stem cells [44]. The downregulation of transcription factor SOX-2 in cancer stem cells (CSCs) was correlated with the suppression of lung cancer growth and metastasis behavior [45]. The SOX-2 knockdown in murine lung carcinoma cells inhibited tumor growth and metastases in C57BL/6 mice in vivo [45]. In gastric tumor cells, Sox-2 and Oct-4 play main roles in the proliferation, migration, invasion, and tumorigenicity [46]. The overexpression of Oct-4 and Sox-2 is linked to a greater migration ability [46]. In the current study, the gene expression of Sox-2 and Oct-4 decreased the HEBR-treated lung cancer cells, especially for H1299 cells ( Figure 5). This result was consistent with the weaker migration ability observed in HEBR-treated H1299 cells compared with A549 cells (Figures 3 and 4).
In summary, we showed that HEBR protein significantly inhibited the proliferation and migration of lung cancer cells in vitro regardless of whether the green light was employed. The phenomenon was positively correlated with the suppression of stemness of CSCs and EMT activity of the lung cancer cells.

Cell Culture
Human epithelial lung cancer cell lines (A549 and H1299) were cultured in the Roswell Park Memorial Institute medium (RPMI 1640, Gibco, Paisley, UK). Human dermal fibroblasts were isolated from the human foreskin (IRB#100-05-251) [32,47], which was maintained in the high-glucose Dulbecco's modified Eagle medium (DMEM, Gibco, Grand Island, NY, USA). The two abovementioned culture media contained 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% Antibiotic-Antimycotic (Gibco, Grand Island, NY, USA). The cultured cells were incubated under the condition of 5% CO 2 at a stable temperature of 37 • C.

Preparation of Purified HEBR Protein
HEBR-protein expression was established by the Escherichia coli (E. coli) system according to previous literature [15,16]. E. coli strain C43 (DE3) was used as the host cell to prepare the protein. The protein concentration was calculated by UV-VIS spectroscopy.

Immunostaining for HEBR Protein in A549 and H1299 Cells
Immunostaining assay was performed by following the procedures previously described [16]. Briefly, 7500 cells were seeded on each well of a 24-well plate overnight. The cells were then treated with HEBR protein (20 µg/mL) for 24 h. After incubation, the cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 15 min. The cells were rinsed with PBS and blocked with 1% bovine serum albumin (BSA) for 1 h. Cells were then incubated with 6X His tag antibody (GTX628914, GeneTex, Hsinchu, Taiwan) at 4 • C overnight, followed by treatment of Alexa Fluor 488 anti-Rabbit (ab150080) secondary polyclonal antibody. Cell nuclei were counterstained with Hoechst 33258. After staining, the coverslip was mounted in Fluoro-Gel (Electron Microscopy Sciences, Hatfield, PA, USA), and the fluorescence was observed by a fluorescence microscope (Nikon EClipse 80i, Tokyo, Japan).

Cell Viability Analysis
The cell viability of A549 or H1299 treated with HEBR protein was further analyzed by the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) assay according to the manufacturer's instructions [16]. A549 or H1299 cells with a density of 1500 cells/well were seeded into 96-well culture plates for 24 h and incubated with several concentrations of HEBR protein in a culture RPMI medium. After incubation for 24 h, the cells were stimulated with (or without) the green (525 nm)-light-emitting diode (LED) for three times per day. Each illumination lasted for 10 s, and the exposure distance between the light source and cultured cell lines was~2 cm. After incubation for 48 h, the medium was switched to the culture medium with 100 µL of the CCK-8 reagent to obtain orange-colored solution. After incubation for 60 min, absorbance at the wavelength of 450 nm was detected. The percentage of cell viability was measured as the percentage of viable cells relative to untreated cells.

Cell Cycle
The cell cycle of A549 was performed as previously described [16]. Briefly, A549 cells were cultured under the serum-deprived RPMI medium for 18 h to synchronize the cell cycle before the HEBR protein treatment. The cells were incubated to the complete culture RPMI medium containing 40 µg/mL HEBR proteins for 24 h. The profile of cell cycle of A549 was determined by a two-step cell cycle assay (Chemometec, Allerød, Denmark) using the NucleoCounter ® NC-3000 TM instrument according to the manufacturer's instructions.

Migration Assay
The migration ability of A549 or H1299 after the treatment treated of HEBR protein was evaluated by the scratch-migration assay and transwell migration assay as previously described [48,49]. For the scratch-migration assay, the cells (2.5 × 10 6 cells/well) were seeded in 24-well plates for 24 h and a gap region was made using a sterile 200 µL pipette tip across the cell monolayer (>90% confluent). HEBR proteins of two different concentrations were incubated with the cells and the gap region was tracked by a time-lapse recording system (Real Time Cell Culture Monitoring (CCM) System, Astec, Fukuoka, Japan ) in RPMI medium. A series of the scratching images was captured from 0 h to 6 h. Quantification of migration rate was carried out using the MRI wound healing tool of NIH Image J software. For transwell migration assay, 4 × 10 5 cells were seeded on each 24 mm-diameter transwell membrane (0.8 µm pore size, Corning, NY, USA) with 1.5 mL serum-free RPMI medium in the upper chamber. In the lower chamber, 2 mL basal culture RPMI medium with 10% FBS was used as a chemoattractant for cells to promote cell migration. Cells were treated with HEBR (20 µg/mL) and incubated for 24 h. The cells that did not migrate were removed by a cotton swab and stained with crystal violet (0.2%). The images of migrated cells were captured under an inverted microscope and the quantification of the cell number was conducted by the cell-counter tool of Image J software.

RNA Extraction and Quantitative Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Analysis
The experiments were carried out as previously described [16]. The total RNA of the A549 or H1299 cells was isolated using the Trizol reagent (Invitrogen, Carlsbad, CA, USA) after the treatment of the HEBR (40 µg/mL) with or without light illumination for 48 h. The RNA strands were extracted by RevertAid™ First Strand cDNA Synthesis Kit (Thermo Scientific, Vilnius, Lithuania) to synthesize complementary DNA (cDNA). The gene expression level was labeled by the KAPA SYBR green fast qPCR Master Mix Kit (KAPA Biosystems, Cape Town, South Africa) and performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Singapore). The EMT and stemness geneexpression markers such as Snail-1, Twist-1, E-cad, N-cad, Sox-2, Oct-4, and CD133 were evaluated after the treatment of HEBR protein. The expression levels were normalized by endogenous housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH).

Statistical Analyses
The experimental data were displayed as the mean ± standard deviation (SD). Each quantitative experiment was independently performed three times for each group. A one-way ANOVA test was conducted to examine the statistical difference for two or more experimental groups, using GraphPad Prism 5 software.

Conclusions
This study demonstrated that the optogenetic HEBR protein significantly influenced lung cancer cell proliferation and cell cycle progression, reduced migration activity, and suppressed some stemness-related genes. The administration of HEBR has a substantially different effect on cancer cells compared to normal cells, which could serve as a novel therapeutic strategy for the treatment of lung cancer metastasis. In the current study, we obtained the optimal concentration range of HEBR to repress the migration activity of cancer cells. In the future, we will use the mice model and combine multidrug treatment to further investigate the effect of HEBR on the progression and migration behavior of tumor. Meanwhile, Sox-2 and Oct-4 are well known to be involved in maintaining the stemness, cancer progression, and the resistance towards cancer therapies of cancer cells. Therefore, HEBR protein may be combined with cancer drugs to suppress the Sox-2 and Oct-4 gene expression of lung cancer stem cells. Taken together, we propose that the HEBR protein may be useful in cancer therapeutics, and the control mechanism of cancer cell migration ability may be independent of light exposure.
Supplementary Materials: Figure S1: Cell viability of human fibroblasts after treatment with HEBR protein for 72 h.

Institutional Review Board Statement:
In this study, primary human dermal fibroblasts were acquired from human foreskin according to the guidelines of the surgery of circumcision in the Tri-Service General Hospital, Republic of China (IRB#100-05-251).
Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.
Data Availability Statement: Not applicable.