New Polyesterified Ursane Derivatives from Leaves of Maesa membranacea and Their Cytotoxic Activity

Maesa membranacea A. DC. (Primulaceae) is a plant species that has been frequently used by practitioners of the traditional ethnobotany knowledge from northern and central Vietnam. However, the chemical constituents of the plant remained unknown until recently. Chromatographic separation of a chloroform-soluble fraction of extract from leaves of M. membranacea led to the isolation of two new polyesterified ursane triterpenes (1–2) and two known apocarotenoids: (+)-dehydrovomifoliol (3) and (+)-vomifoliol (4). The chemical structures of the undescribed triterpenoids were elucidated using 1D and 2D MNR and HRESIMS spectral data as 2α,6β,22α-triacetoxy-11α-(2-methylbutyryloxy)-urs-12-ene-3α,20β-diol (1) and 2α,6β,22α-triacetoxy-urs-12-ene-3α,11α,20β-triol (2). The newly isolated triterpenoids were tested for their cytotoxic activity in vitro against two melanoma cell lines (HTB140 and A375), normal skin keratinocytes (HaCaT), two colon cancer cell lines (HT29 and Caco-2), two prostate cancer cell lines (DU145 and PC3) and normal prostate epithelial cells (PNT-2). Doxorubicin was used as a reference cytostatic drug. The 2α,6β,22α-triacetoxy-11α-(2-methylbutyryloxy)-urs-12-ene-3α,20β-diol demonstrated cytotoxic activity against prostate cancer cell lines (Du145—IC50 = 35.8 µg/mL, PC3—IC50 = 41.6 µg/mL), and at a concentration of 100 µg/mL reduced viability of normal prostate epithelium (PNT-2) cells by 41%.


Introduction
The over 30 plant species that are included in the genus Maesa are all native to tropical areas of the Old World [1]. Maesa was previously classified into the Myrsinaceae family, and although it has been postulated that the taxon should be the only member of the newly created Maesaceae family [2], current botanical databases [1] classify it as a member of the Primulaceae. Plants of the genus have traditionally been used as anthelminthic and antiviral remedies in both Africa and Southeast Asia [3][4][5][6][7][8][9][10]. Preparations from Maesa lanceolata have also been taken as nerve-stimulants and as memory restorers [5,6]. Phytochemical studies on Maesa spp. have led to the isolation of oleanane-type saponins, benzoquinones and flavonoids [11][12][13][14][15][16].
The objective of the present study was to investigate composition of a chloroform fraction of extract from M. membranacea leaves in a search for new biologically active compounds.
The objective of the present study was to investigate composition of a chloroform fraction of extract from M. membranacea leaves in a search for new biologically active compounds.

Results
Two new ursanes (1 and 2) and two known apocarotenoids (3 and 4) (for the structures see Figure 1) were isolated from the chloroform soluble fraction of the methanolic extract from the leaves of M. membranacea.

Structure Elucidation
Compound 1 was isolated as white crystals. The HRESIMS spectrum of 1 showed an adduct ion peak m/z 739.4401 [M + Na] + that corresponded to the molecular formula of C 41 H 64 O 10 Na (calculated mass 739.4397). The molecular formula of 1, established as C 41 H 64 O 10 (Figure 1), indicated ten degrees of unsaturation, attributed to five ring systems, one olefinic double bond and four ester carbonyl groups.
The 13 C NMR (Table 1) and HSQC spectral data indicated the presence of fortyone carbons assigned to thirteen methyl groups, six methylenes, ten methines including one olefinic and five hydroxylated or esterified groups (δ C 70. 05 .61, respectively. HMBC cross peaks made it possible to connect the carbonyl at δ C 176.28 with 2 -methylbutyryl group and the remaining carbonyls to the respective methyls of the acetyl groups. The remaining thirty signals at 13 C NMR spectrum, including six tertiary and two secondary methyl groups not ascribed to the four acyl functions, corresponded to a polyhydroxylated urs-12-ene triterpenoid [23][24][25]. HMBC experiment showed the correlation of methyl protons at C-23 (δ H 1.10) and C-24 (δ H 1.13) with C-3 (δ C 77.49), which has a hydroxyl group. Correlation signals from δ H 3.49 (H-3) to δ H 5.29 (H-2), in 1 H-1 H COSY spectrum, suggested the location of acetyl group at C-2. This was supported by the HMBC correlation of quaternary carbon of acetyl group at δ C 169.71 with H-2. Values of proton coupling constants of H-2 together with a small coupling constant of H-3 indicated 2α,3α orientation of the substituents at C-2 and C-3 [26]. Cross peak of C-1 at δ C 176.28 and H-11 and cross peak of quaternary carbon of acetyl group at δ C 169.  (Table 1).  Compound 2 was isolated as an amorphous solid. HRESIMS spectrum of 2 showed an adduct ion peak m/z 655.3824 [M + Na] + that corresponded to the molecular formula of C36H56O9Na (calculated mass 655.3822). The molecular formula of 2, established as C36H56O9 (Figure 1), indicated nine degrees of unsaturation that can be attributed to five ring systems, one olefinic double bond and three ester carbonyl groups.
Compound 2 was isolated as an amorphous solid. HRESIMS spectrum of 2 showed an adduct ion peak m/z 655.3824 [M + Na] + that corresponded to the molecular formula of C 36 H 56 O 9 Na (calculated mass 655.3822). The molecular formula of 2, established as C 36 H 56 O 9 (Figure 1), indicated nine degrees of unsaturation that can be attributed to five ring systems, one olefinic double bond and three ester carbonyl groups. The 13 C NMR (Table 2) and HSQC spectral data indicated the presence of thirtysix carbons assigned to eleven methyl groups, five methylenes, nine methines including five hydroxylated or esterified groups (δ C 67.85, 70.26, 70.37, 77.52 and 78.53) and eleven quaternary carbons including three carbonyls (δ C 170.34, 170.05 and 169.40) and one oxygenated quaternary carbon (δ C 71.28). Signals at δ C 129.83 and 140.08 indicated the presence of the double bond. The 1 H NMR spectrum (Table 2) showed three singlets at δ H 2.07, 2.08 and 2.12 (3H each) corresponding to three acetyl groups. Based on HSQC correlations the singlets could be connected to the corresponding carbons at δ C 21.28, 21.87 and 21.38, respectively. HMBC experiment showed the correlation of methyl protons at C-23 (δ H 1.11) and C-24 (δ H 1.15) with C-3 (δ C 77.52) which has a hydroxyl substituent.  (Table 2).    . Figure 3. Key NOESY correlations for 2.

Cytotoxic Activity
Cytotoxicities of 1 and 2, at a dose range of 5-100 µg/mL, were tested against three panels of human cancer and normal cells (see Table 3 and Table S1 in the Supplementary Materials). In the skin panel, both compounds exerted weak cytotoxicity towards HTB140 and A375 melanoma cells (at 100 µg/mL of 1, viability of the most susceptible cell line HTB140 exceeded 45%) and keratinocytes (100 µg/mL, over 54% viable cells), after 24 h treatment. Compound 2, in general, was less active than 1, against all cancer cell lines used in the study. The two colon cancer cell lines used in the experiment demonstrated different sensitivities to the treatment with 1. The triterpene was modestly toxic to Caco-2 cells (IC50-35.7 µg/mL) whereas the line HT29 was less susceptible (100 µg/mL, over 55% viable cells). Prostate cancer cell lines of different metastatic potential were used for the cytotoxicity assessment. Compound 1 was less effective (IC50-41.6 µg/mL) against PC3 cells (with high metastatic potential) than against DU145 cells (IC50-35.8 µg/mL). The normal prostate epithelial cells PNT-2 showed over 59% viability after 24 h of treatment with 100 µg/mL of 1 and over 93% viability after the treatment with 100 µg/mL of 2, which indicates selective activity of the compounds. Though IC50 values for the compound 2 exceeded the dose of 50-100 µg/mL, the compound demonstrated cytotoxicity profile similar to that of 1 (Table S1) with the highest activity towards Caco-2 and PC3 cells. Similar to 1, compound 2 was less active against prostate normal epithelial cells than against the DU 145 and PC3 prostate cancer cells (over 57% and 47% On the basis of these results, compound 2 was deduced to be 2α,6β,22α-triacetoxy-urs-12-ene-3α,11α,20β-triol, a new natural product. The known compounds 3 and 4 were identified as (+)-dehydrovomifoliol and (+)-vomifoliol, respectively, by comparison of their spectral data with that found in the literature [27,28].

Cytotoxic Activity
Cytotoxicities of 1 and 2, at a dose range of 5-100 µg/mL, were tested against three panels of human cancer and normal cells (see Table 3 and Table S1 in the Supplementary Materials). In the skin panel, both compounds exerted weak cytotoxicity towards HTB140 and A375 melanoma cells (at 100 µg/mL of 1, viability of the most susceptible cell line HTB140 exceeded 45%) and keratinocytes (100 µg/mL, over 54% viable cells), after 24 h treatment. Compound 2, in general, was less active than 1, against all cancer cell lines used in the study. The two colon cancer cell lines used in the experiment demonstrated different sensitivities to the treatment with 1. The triterpene was modestly toxic to Caco-2 cells (IC 50 -35.7 µg/mL) whereas the line HT29 was less susceptible (100 µg/mL, over 55% viable cells). Prostate cancer cell lines of different metastatic potential were used for the cytotoxicity assessment. Compound 1 was less effective (IC 50 -41.6 µg/mL) against PC3 cells (with high metastatic potential) than against DU145 cells (IC 50 -35.8 µg/mL). The normal prostate epithelial cells PNT-2 showed over 59% viability after 24 h of treatment with 100 µg/mL of 1 and over 93% viability after the treatment with 100 µg/mL of 2, which indicates selective activity of the compounds. Though IC 50 values for the compound 2 exceeded the dose of 50-100 µg/mL, the compound demonstrated cytotoxicity profile similar to that of 1 (Table S1) with the highest activity towards Caco-2 and PC3 cells. Similar to 1, compound 2 was less active against prostate normal epithelial cells than against the DU 145 and PC3 prostate cancer cells (over 57% and 47% viable cells, respectively, after the treatment with 100 µg/mL of 2).

Discussion
Triterpenoids of ursane and oleanane type are ubiquitous plant constituents. According to the published phytochemical studies, plants from the Primulaceae family synthesize oleanane derivatives almost exclusively. In fact, we found only two papers that dealt with ursane-type triterpenoid isolation from the plants included in the family. The first one was on saponin-clethroidoside H separation from Lysimachia clethroides Duby [29], and the second was on extraction of ursolic acid from the stem wood of Maesa lanceolata Forssk. [30]. Ursanes are not rare in plants from the other families of Ericales (e.g., Actinidiaceae, Ericaceae and Lecythidaceae) and the hydroxyl groups at 2α, 3α and 6β are not unique there [31][32][33]. Polyhydroxylated ursanes with substitution pattern similar to 1 were described earlier as constituents of Salvia argentea L. (Lamiaceae) [22] and Siphonodon celastrineus Griff. (Celastraceae) [34]. In both cases, however, the hydroxyl group at C-3 was β oriented. Our results clearly indicate α-orientation of the hydroxyl group in 1 and 2.
Cytotoxicity of 1 and 2 was tested against three panels of human cancer and normal cells (Table 3 and Table S1). In general, compound 1 demonstrated higher activity against all cell lines used in the study. As the only difference in structure between 1 and 2 was the presence or absence of 2-methylbutyryl group bonded to the oxygen at C-11, it may suggest that the lower polarity of the molecule and/or its shape is responsible for the more pronounced cytotoxicity of 1. Selectivity in the cytotoxic effect of 1 towards the prostate cancer cells as well as the activity of the triterpene against doxorubicin-resistant cells (PC3 line) is worth noting.
Ursane-type triterpenes with multiple oxygen functionalities frequently demonstrated cytotoxic activity towards human and murine cancer cells in vitro [41][42][43][44][45]. The described IC 50 values vary broadly (5.7-57.0 µM), depending on the structure of the investigated compound, the cell line used, and the time of exposure applied (24-96 h). This makes the direct comparison of the results difficult. Some remarks on relationships between the chemical structure and the biological activity of substituted ursolic acid derivatives have been summarized by Sommerwerk et al. [45]. They concluded that ursanes were, in general, less active than oleananes with the corresponding substitution pattern. The two acetyl groups at C-2 and C-3 were in favor of cytotoxicity; however, their optimum configuration should be 2β,3β (contrary to 2α,3α in 1 and 2).
Apocarotenoids: (+)-dehydrovomifoliol (3) and (+)-vomifoliol (4) are biologically active compounds frequently found in the aerial parts of plants from different taxonomic groups. However, their presence in Maesa ssp. has not been described yet.

General Experimental Procedures
NMR spectra were recorded in CDCl 3 on a Bruker AVANCE III HD 400 (resonance frequency 400.17 MHz for 1 H and 100.63 MHz for 13 C) spectrometer (Bruker Corp., Billerica, MA, USA). High resolution mass spectra were obtained in the positive ion mode using MaldiSYNAPT G2-S HDMS (Waters Inc., Milford, MA, USA) mass spectrometer equipped with an electrospray ion source and Q-TOF type mass analyzer. Optical rotation was determined in CDCl 3 on a PolAAr31 polarimeter (Optical Activity Ltd., Huntingdon, England). RP-HPLC separations were performed using an Agilent 1200 Series HPLC system (Agilent Technologies Inc., Santa Clara, CA, USA) equipped with a diode array detector. Analytical chromatographic separations were carried out on a Kinetex XB-C18 column (4.6 × 250 mm, 5 µm total particle size; Phenomenex Inc., Torrance, CA, USA). Semipreparative RP-HPLC was conducted on a Vertex Plus Eurospher II 100-5 C18 column (250 × 8 mm; Knauer GmbH, Berlin, Germany), with an isocratic elution, using methanol-water (MeOH-H 2 O) mixtures of different polarity. Conventional column chromatography (CC) was carried out on Silica gel 60 (0.063-0.2 mm, Merck KGaA, Darmstadt, Germany). Thin-layer chromatography (TLC) separations were performed using precoated plates (Silica gel 60 without fluorescence indicator, Art. No 5553, Merck, Darmstadt, Germany). Solvents of analytical grade were purchased from Avantor Performance Materials S.A. (Gliwice, Poland). Water was purified by a Mili-Q system (Milipore Corp., Bedford, MA, USA). MeOH and MeCN of HPLC grade were bought from Merck (Darmstadt, Germany).

Plant Material
Leaves of M. membranacea A. DC. were collected from the Kontum province (Vietnam), and were taxonomically verified by Dr. Nguyen Quoc Binh from the Vietnam Museum of Nature of the Vietnam Academy of Science and Technology (VAST). A voucher specimen (VN-2292) has been deposited in the Institute of Marine Biochemistry VAST in Hanoi.

Extraction and Isolation of 1-4
Coarsely ground dried leaves of M. membranacea (665 g) were extracted, as previously described [22], with 80% MeOH (5 × 4 L) in room temperature. The obtained extracts were concentrated in vacuo to yield 193.5 g of an oily residue. The residue was suspended in water (1 L) and subsequently partitioned with solvents of increasing polarity. The chloroform fraction of the extract (5.9 g) was subjected to CC over silica gel (104 g) using mobile phase gradients of ethyl acetate (EtOAc) in hexane (up to 100% EtOAc; fractions 1-396) and MeOH in EtOAc (up to 20% MeOH; fractions 397-424). The separated fractions (50 mL each) were monitored by TLC (supported by RP-HPLC, if necessary) and the relevant ones were combined. Fractions 117-122 (eluted with hexane-EtOAc; 3:2, v/v), after purification by preparative TLC (precoated TLC plates, solvent system: hexane-EtOAc,  The examined triterpenes were diluted in the culture media from freshly made stock solutions in MeOH (10 mg/mL) to the working concentrations (from 0 to 100 µg/mL).
Cell viability was determined as it was described previously [46]. Cells suspended in the nutrient medium were transferred into 96-well microtiter plates (density 1.5 × 10 4 per well), and preincubated for 24 h (37 • C, 5% CO 2 ). Then, the culture medium was replaced with the medium containing different concentrations of 1 or 2 (1-100 µg/mL). After 24 h of incubation, viability of the cells was determined using colorimetric lactate dehydrogenase (LDH) assay, in comparison to the controls to which corresponding aliquots of MeOH diluted with culture media were added. Cells grown in the medium without the tested compounds were used as control I (negative) and the positive control (control II) was obtained by incubation of the cells in the medium containing 1% Triton X-100. LDH released from the damaged cells into the cell culture medium was quantified by measuring the absorbance at 490 nm using Synergy II Biotek (BioTek Instruments, Winooski, VT, USA) microplate reader. Cytotoxicities of the examined compounds were calculated as follows: [(absorbance of the tested sample − absorbance of control I)/(absorbance of control II − absorbance of control I)] × 100. Results were means of three independent measurements (± SD). Doxorubicin (Ebewe Pharma GmbH., Unterach, Austria) was used as a reference cytostatic drug. The IC 50 values were determined by plotting the percentage viability of the cells versus concentration and the adequate calculations made using AAT Bioquest website program (https://www.aatbio.com/tools/ic50-calculator, accessed on 29 October 2021).

Conclusions
The isolated polyesterified triterpenes represent the structural type of the compounds with unique substitution pattern. Polyfunctionalized triterpenes of ursane-type have not been previously found in Maesa spp. and are very rare in the Primulaceae. They seem to be good systematic markers of M. membranacea.
Supplementary Materials: The following are available online, Figures S1-S14: HR ESIMS and NMR spectra of 1 and 2, Figures S15 and S16: 1 H-NMR spectra of 3 and 4, Table S1: Viability of human normal and cancer cell lines treated for 24 h with 5-100 µg/mL of 1 or 2.

1D NMR
One-dimensional nuclear magnetic resonance spectroscopy 2D NMR Two-dimensional nuclear magnetic resonance spectroscopy CC Conventional column chromatography CDCl 3 Deuterated