Dibenzofuran Derivatives Inspired from Cercosporamide as Dual Inhibitors of Pim and CLK1 Kinases

Pim kinases (proviral integration site for Moloney murine leukemia virus kinases) are overexpressed in various types of hematological malignancies and solid carcinomas, and promote cell proliferation and survival. Thus, Pim kinases are validated as targets for antitumor therapy. In this context, our combined efforts in natural product-inspired library generation and screening furnished very promising dibenzo[b,d]furan derivatives derived from cercosporamide. Among them, lead compound 44 was highlighted as a potent Pim-1/2 kinases inhibitor with an additional nanomolar IC50 value against CLK1 (cdc2-like kinases 1) and displayed a low micromolar anticancer potency towards the MV4-11 (AML) cell line, expressing high endogenous levels of Pim-1/2 kinases. The design, synthesis, structure–activity relationship, and docking studies are reported herein and supported by enzyme, cellular assays, and Galleria mellonella larvae testing for acute toxicity.


Introduction
Cercosporamide is a natural product isolated from pathogen fungus Cercosporidium henningsii, commonly found in extensively cultivated plants (Manihot esculenta) for domestic consumption of tapioca in tropical and subtropical regions worldwide [1]. Moreover, this substance can be found in cultures of Phoma fungi isolated from Guinea plant Saurauia scaberrinae [2] and Chinese medicinal plant Arisaema erubescensor [3], or can be produced by fungi of the genus Lachnum and Pseudaegerita [4]. As the main structural features,

Synthesis
The synthetic strategies to prepare dibenzofurans can be classified into two main categories [37][38][39][40][41] (Figure 2). One involves the construction of the dibenzofuran from the ring closure of diaryl ethers through C−C bond formation [37,38]. The second approach refers to the intramolecular O-arylation of 2-arylphenols according to a mechanism of etherification [40,41]. Since the discovery of cercosporamide, important findings concerning its biological activities have been reported. In 2004, Sussman et al. [5] reported cercosporamide as a potent selective inhibitor of Candida albicans CaPkc1 (PKC-like 1 kinase), which is central to fungal cell wall integrity and was validated as a fungal drug target in cases where drug resistance is evident. In addition, according to the literature [6], cercosporamide was shown to be a potent ATP-competitive CaPkc1 inhibitor with IC 50 = 44 nM. In the last decade, nanomolar range inhibition of MAPK-interacting kinases (Mnk1/2) by cercosporamide has been shown to be an efficient alternative to block the activation of the translation initiation factor 4E (eIF4E) pathway and consequently to prevent tumor growth and progression [7,8]. In this way, activity against lung cancer [8], acute myeloid leukemia [9], and hepatocellular carcinoma [10] have been achieved by employing cercosporamide as an Mnk1/2 kinase inhibitor. In addition, inhibition of Mnk pathways by cercosporamide enhanced glioblastoma cell response to chemotherapy and radionuclide therapy [11]. Recent results in the zebrafish model, through the inhibition of bone morphogenetic protein receptor (BMPR) type I kinase, suggest that cercosporamide represents a potential therapeutic strategy to combat diseases with overactive BMPR signaling, including the rare genetic disorder fibrodysplasia ossificans progressiva (FOP) and the rare childhood brainstem tumor diffuse intrinsic pontine glioma (DIPG) [12].
In addition, cercosporamide-inspired synthetic compounds have also been reported. Furukawa et al. described the preparation of novel antihyperglycemic agents from cercosporamide [13]. In this work, a potent plasma glucose-lowering effect was observed in hyperglycemic KK/Ta mice. The same group completed the previous work by designing cercosporamide derivatives endowed with an antidiabetic effect and attenuated adverse effects as novel selective PPARγ modulators [14].
With the aim of developing new heterocyclic compounds displaying biological activities, our research group also validated cercosporamide as a valuable model in the drug design strategy. Indeed, benzofuran derivatives exhibited promising antiproliferative activity against two human non-small cell lung cancer (NSCLC) cell lines [15]. More recently, benzofuro [3,2-d]pyrimidines proved to be interesting agents in antifungal chemotherapy by restoring the susceptibility of resistant strains to azole treatment [16].
Although research on the biological activity of cercosporamide has increased in the last decade, few efforts have been carried out in the design of kinase inhibitor deriva-kinase. In positions 7 (R2) and 8 (R3) of the molecule, additional hydroxyl and/or acetyl groups present in cercosporamide and usnic acid could also promote the binding of the designed compounds. Other modulations, including nitro, amino, fluoro, and trifluoromethyl groups, were also considered for the structure-activity relationship (SAR) study.
Taking into account the biological activity of the structural models used in the study, the main goal of the project was to obtain novel Pim-1/Pim-2 kinase inhibitors as anticancer agents. Indeed, the overexpression of serine/threonine Pim kinases (proviral integration site for Moloney murine leukemia virus kinases), in various types of hematological malignancies and solid carcinomas, is characterized in the literature, and these proteins promote cell proliferation and survival [29][30][31]. Consequently, Pim kinases were considered as a valuable target for antitumor therapy [32][33][34][35]. Interestingly, until now, dibenzofurans have not been described in the literature as Pim kinase inhibitors [21,[29][30][31]34,36].
Eight new 1,3-dihydroxydibenzo[b,d]furan-4-carboxamide derivatives were screened against Pim-1 and Pim-2 and a panel of seven additional mammalian protein kinases to check their selectivity profile. The corresponding methoxy precursors were also evaluated for their kinase inhibition properties to complete the SAR study. Antiproliferative activities of the most promising kinase inhibitors were further tested against seven different cell lines. In complement, the determination of in vivo toxicity using Galleria mellonella larvae was performed. Finally, information of ligand-protein interactions was provided through docking study on Pim-1.

Synthesis
The synthetic strategies to prepare dibenzofurans can be classified into two main categories [37][38][39][40][41] (Figure 2). One involves the construction of the dibenzofuran from the ring closure of diaryl ethers through C−C bond formation [37,38]. The second approach refers to the intramolecular O-arylation of 2-arylphenols according to a mechanism of etherification [40,41].

. Access to Dibenzofuran Derivatives by Intramolecular C-C Bond Formation from Diaryl Ethers
The first method involves ortho-(aryloxy)aryldiazonium salts as intermediates to form an intramolecular C-C bond via a free-radical cyclization ( Figure 2) [42][43][44]. Before obtaining the diazonium salt, the advantage of this approach relates to the nitro function that promotes the access to diaryl ethers 5-8 during the initial SN Ar reaction between 1-iodo-2-nitrobenzene and phenolic derivatives 1-4 (Scheme 1).
The standard copper-catalyzed Ullmann diaryl ether synthesis [45,46] was first performed in the presence of the previously synthesized phenol derivative 1 [16] and its dimethyl analogue 2, obtained in the same conditions from commercially available 3,5-dimethoxyphenol 4. This route could allow the introduction of the carboxamide function before the heterocyclization step. Unfortunately, no reaction occurred for the dibenzylated phenol 1, and its dimethyl counterpart 2 gave only a poor yield of 15% after the one-day reaction (Scheme 1). Modification of the combination of the copper source (CuI), base (Cs2CO3, NaH), and additional ligand (N,N-dimethylglycine) did not improve the completion of the reaction. Interestingly, the same reaction carried out from phenols 3 [16] and 4, without carboxamide substitution, afforded the desired diaryl ethers 7 and 8 in good yields (73% and 78%, respectively). This result indicated that the reaction is more sensitive to the electronic-withdrawing effect of the carboxamide and the steric hindrance in the ortho position of the phenol than to the bulky benzyl-protecting groups.
The standard copper-catalyzed Ullmann diaryl ether synthesis [45,46] was first performed in the presence of the previously synthesized phenol derivative 1 [16] and its dimethyl analogue 2, obtained in the same conditions from commercially available 3,5dimethoxyphenol 4. This route could allow the introduction of the carboxamide function before the heterocyclization step. Unfortunately, no reaction occurred for the dibenzylated phenol 1, and its dimethyl counterpart 2 gave only a poor yield of 15% after the one-day reaction (Scheme 1). Modification of the combination of the copper source (CuI), base (Cs 2 CO 3 , NaH), and additional ligand (N,N-dimethylglycine) did not improve the completion of the reaction. Interestingly, the same reaction carried out from phenols 3 [16] and 4, without carboxamide substitution, afforded the desired diaryl ethers 7 and 8 in good yields (73% and 78%, respectively). This result indicated that the reaction is more sensitive to the electronic-withdrawing effect of the carboxamide and the steric hindrance in the ortho position of the phenol than to the bulky benzyl-protecting groups.
Nitro-diaryl ethers 7 and 8 were further reduced into the corresponding anilines 9 and 10 in a moderate to good yield, respectively (Scheme 1). In the presence of zinc dust and ammonium chloride [47] in methanol, for better solubility, it was necessary to warm the medium to 80 • C to achieve a complete conversion of the hydroxylamine intermediate to the amino group. Cyclization of the compounds 9 and 10 proceeded after transformation of the amino function into the corresponding diazonium salts, which were then heated with copper powder [48] or palladium acetate [37] to form the dibenzo[b,d]furans 11 and 12. Unfortunately, the conversion rates, measured by UPLC-MS analysis, to the target compounds remained very low (6 to 28%), limiting the application of this route further. The major products of the reaction were the corresponding diaryl ethers with loss of the amino group.
However, this synthetic route enabled us to obtain a very useful intermediate compound 8 to ensure the continuation of the project. Indeed, taking into account our literature survey, an intramolecular palladium(II)-catalyzed oxidative carbon-carbon bond formation, according to Fagnou's conditions [49,50], would allow us to access a nitrodibenzofuran derivative 13 whose nitro function is easily replaced by a hydrogen via the reduction of the corresponding diazonium salt (Scheme 2). Additionally, this approach has the advantage of functionalizing the dibenzofuran scaffold with a nitro group, providing functional diversity, after reduction to the corresponding amine for instance. However, this synthetic route enabled us to obtain a very useful intermediate compound 8 to ensure the continuation of the project. Indeed, taking into account our literature survey, an intramolecular palladium(II)-catalyzed oxidative carbon-carbon bond formation, according to Fagnou's conditions [49,50], would allow us to access a nitrodibenzofuran derivative 13 whose nitro function is easily replaced by a hydrogen via the reduction of the corresponding diazonium salt (Scheme 2). Additionally, this approach has the advantage of functionalizing the dibenzofuran scaffold with a nitro group, providing functional diversity, after reduction to the corresponding amine for instance. Following Pd-mediated CH activation conditions, the dibenzofuran ring closure was performed, from nitro-diaryl ether 8, in the presence of silver acetate (AcOAg) in pivalic acid as solvent at 130 °C with a 72% yield. Afterwards, a direct aminocarbonylation at the C-4 position of the dibenzofuran derivative 14, using chlorosulfonyl isocyanate (CSI) as an electrophilic reagent, led to compound 14 after acidic hydrolysis of the corresponding N-chlorosulfonylcarboxamide intermediate [15,16,51]. Demethylation of the methoxy groups was then carried out by heating compound 14 in neat pyridine hydrochloride at 200 °C, under microwave irradiation (MW), to lead in a short time (15 min) to the dihydroxy derivative 15, available for biological testing [52]. The nitro function was then reduced by using zinc and ammonium chloride, as described above, to afford the final amino compound 16. Finally, deamination of dibenzofuran derivative 16, via the thermal decomposition of the corresponding diazonium salt with the release of nitrogen, furnished the unsubstituted target compound 17. To scope the synthetic approach further, the nitro derivative 14 was reduced to afford 18 as a precursor of the dimethoxy derivative 19, which could give compound 17 by an alternative route, after demethylation reaction. By applying such a three-step procedure from compound 14, the global yield decreased by comparison to the initial strategy (11% vs. 16.5%) but the dimethoxy precursors 14, 18, and 19 of the dihydroxy-dibenzofurans 15, 16, and 17, respectively, were obtained for complete SAR study.
According to Liégault et al. [49], the ortho-directed CH activation of diaryl ethers, discussed above for accessing dibenzofurans, tolerates a broad substrate scope including both electron-rich and electron-deficient derivatives. Consequently, this approach was envisaged for pharmacomodulation of R1, R2, and R3 of the additional target compounds ( Figure 1). The first step consisted of the synthesis of the suitable aryl iodide derivatives Following Pd-mediated CH activation conditions, the dibenzofuran ring closure was performed, from nitro-diaryl ether 8, in the presence of silver acetate (AcOAg) in pivalic acid as solvent at 130 • C with a 72% yield. Afterwards, a direct aminocarbonylation at the C-4 position of the dibenzofuran derivative 14, using chlorosulfonyl isocyanate (CSI) as an electrophilic reagent, led to compound 14 after acidic hydrolysis of the corresponding N-chlorosulfonylcarboxamide intermediate [15,16,51]. Demethylation of the methoxy groups was then carried out by heating compound 14 in neat pyridine hydrochloride at 200 • C, under microwave irradiation (MW), to lead in a short time (15 min) to the dihydroxy derivative 15, available for biological testing [52]. The nitro function was then reduced by using zinc and ammonium chloride, as described above, to afford the final amino compound 16. Finally, deamination of dibenzofuran derivative 16, via the thermal decomposition of the corresponding diazonium salt with the release of nitrogen, furnished the unsubstituted target compound 17. To scope the synthetic approach further, the nitro derivative 14 was reduced to afford 18 as a precursor of the dimethoxy derivative 19, which could give compound 17 by an alternative route, after demethylation reaction. By applying such a three-step procedure from compound 14, the global yield decreased by comparison to the initial strategy (11% vs. 16.5%) but the dimethoxy precursors 14, 18, and 19 of the dihydroxy-dibenzofurans 15, 16, and 17, respectively, were obtained for complete SAR study.
According to Liégault et al. [49], the ortho-directed CH activation of diaryl ethers, discussed above for accessing dibenzofurans, tolerates a broad substrate scope including both electron-rich and electron-deficient derivatives. Consequently, this approach was envisaged for pharmacomodulation of R 1 , R 2 , and R 3 of the additional target compounds (Figure 1). The first step consisted of the synthesis of the suitable aryl iodide derivatives (Scheme 3) for the preparation of starting diaryl ethers (Scheme 4). To this end, the acetylation of 3-iodophenol 20 was readily achieved with acetic anhydride in pyridine [15] to offer phenyl acetate derivative 21, which was engaged in a Fries rearrangement reaction in the presence of boron trifluoride-acetic acid complex (BF 3 .2CH 3 COOH) to give 2-hydroxyacetophenone 22 in a good yield of 88% [53]. The protection of the phenol moiety was then carried out using iodomethane in basic conditions to obtain compound 23 in good yield (Scheme 4). The same protocol was employed to obtain 3-iodoanisole 24 from 3-iodophenol 20.
Molecules 2021, 26, 6572 6 of 32 (Scheme 3) for the preparation of starting diaryl ethers (Scheme 4). To this end, the acetylation of 3-iodophenol 20 was readily achieved with acetic anhydride in pyridine [15] to offer phenyl acetate derivative 21, which was engaged in a Fries rearrangement reaction in the presence of boron trifluoride-acetic acid complex (BF3.2CH3COOH) to give 2-hydroxyacetophenone 22 in a good yield of 88% [53]. The protection of the phenol moiety was then carried out using iodomethane in basic conditions to obtain compound 23 in good yield (Scheme 4). The same protocol was employed to obtain 3-iodoanisole 24 from 3-iodophenol 20. Diaryl ethers 28-32 were furnished by Ullmann-type coupling between 3,5-dimethoxyphenol 4 and synthesized (23,24) or commercially available (25)(26)(27) aryl iodides (Scheme 4) [50]. It should be noted that the presence of a methoxy group on aryl halides has a deleterious effect for the reaction yield when comparing compounds 28, 29 (30-34%), and 30-32 (70-76%). Otherwise, the addition of N,N-dimethylglycine ligand in the mixture, to stabilize the copper complex, or increased reaction time did not improve the yields. Afterwards, the general reaction sequence (Scheme 4) was the same as previously discussed: (1) Pd-catalyzed CH activation for the dibenzofuran ring closure, from moderate to good yields (compounds 33-37); (2) introduction of the carbamoyl group, in low yields, through electrophilic attack of CSI and acidic hydrolysis (compounds 38-42); and (3) demethylation using pyridinium hydrochloride, in poor yields (compounds 43-47) due to difficulties in the purification on column chromatography. Interestingly, Friedel-Crafts acetylation performed on compound 39, in the presence of acetyl chloride and aluminum trichloride in 1,2-dichloroethane, allowed isolation of compound 38 with a good yield of 74% (Scheme 4) [54]. The proton NMR data validated the regioselective SEAr at the position 8 of the heterocycle. (v) AcCl, AlCl3, Cl-CH2CH2Cl, rt, 1 h, 74%.

Access to Dibenzofuran Derivatives by Intramolecular C-O Bond Formation from 2-Aryl Phenols
In a final strategy for the access to the dibenzo[b,d]furan scaffold, we were interested in exploring the carbon-oxygen bond formation of 2-aryl phenols in our series [52]. In addition, it gave us the opportunity to confirm the regioselectivity of the carbamoyl introduction at the C4-position of the dibenzofuran ring by performing the reaction from benzamide derivative 2, where the CONH2 group was already installed (Scheme 5).
Diaryl ethers 28-32 were furnished by Ullmann-type coupling between 3,5-dimethoxyphenol 4 and synthesized (23,24) or commercially available (25)(26)(27) aryl iodides (Scheme 4) [50]. It should be noted that the presence of a methoxy group on aryl halides has a deleterious effect for the reaction yield when comparing compounds 28, 29 (30-34%), and 30-32 (70-76%). Otherwise, the addition of N,N-dimethylglycine ligand in the mixture, to stabilize the copper complex, or increased reaction time did not improve the yields. Afterwards, the general reaction sequence (Scheme 4) was the same as previously discussed: (1) Pd-catalyzed CH activation for the dibenzofuran ring closure, from moderate to good yields (compounds 33-37); (2) introduction of the carbamoyl group, in low yields, through electrophilic attack of CSI and acidic hydrolysis (compounds 38-42); and (3) demethylation using pyridinium hydrochloride, in poor yields (compounds 43-47) due to difficulties in the purification on column chromatography. Interestingly, Friedel-Crafts acetylation performed on compound 39, in the presence of acetyl chloride and aluminum trichloride in 1,2-dichloroethane, allowed isolation of compound 38 with a good yield of 74% (Scheme 4) [54]. The proton NMR data validated the regioselective SE Ar at the position 8 of the heterocycle.

Access to Dibenzofuran Derivatives by Intramolecular C-O Bond Formation from 2-Aryl Phenols
In a final strategy for the access to the dibenzo[b,d]furan scaffold, we were interested in exploring the carbon-oxygen bond formation of 2-aryl phenols in our series [52]. In addition, it gave us the opportunity to confirm the regioselectivity of the carbamoyl introduction at the C4-position of the dibenzofuran ring by performing the reaction from benzamide derivative 2, where the CONH 2 group was already installed (Scheme 5). (v) AcCl, AlCl3, Cl-CH2CH2Cl, rt, 1 h, 74%.

Access to Dibenzofuran Derivatives by Intramolecular C-O Bond Formation from 2-Aryl Phenols
In a final strategy for the access to the dibenzo[b,d]furan scaffold, we were interested in exploring the carbon-oxygen bond formation of 2-aryl phenols in our series [52]. In addition, it gave us the opportunity to confirm the regioselectivity of the carbamoyl introduction at the C4-position of the dibenzofuran ring by performing the reaction from benzamide derivative 2, where the CONH2 group was already installed (Scheme 5). From this perspective, mono-iodination of compound 2 was first realized in the presence of N-iodosuccinimide to afford ortho-iodophenol derivative 48 [16]. In the next step, we followed the strategy described by Jepsen et al. [52] consisting of the introduction of an ortho-fluorophenyl for subsequent intramolecular cyclisation. Thus, the palladium-catalyzed Suzuki-Miyaura coupling between 2-fluorophenylboronic acid and compound 48, in the presence of palladium acetate, triphenylphosphine, and potassium phosphate as a base, provided the expected compound 49 [55]. Unfortunately, the yield remained very low (26%), but a preliminary attempt of C-C bond formation using Jepsen's conditions under microwave irradiation (PdCl2(PPh3)2, K2CO3, DME/H2O, 80 °C, MW, 2 h) [52] proved to be unsuccessful with degradation of the reaction mixture.
Finally, the reaction of biaryl 49 with cesium carbonate at 150 °C in acetonitrile, under microwave irradiation, furnished compound 19, involving a fluorine atom as the leaving group for nucleophilic substitution. The NMR data and physicochemical properties of the From this perspective, mono-iodination of compound 2 was first realized in the presence of N-iodosuccinimide to afford ortho-iodophenol derivative 48 [16]. In the next step, we followed the strategy described by Jepsen et al. [52] consisting of the introduction of an ortho-fluorophenyl for subsequent intramolecular cyclisation. Thus, the palladiumcatalyzed Suzuki-Miyaura coupling between 2-fluorophenylboronic acid and compound 48, in the presence of palladium acetate, triphenylphosphine, and potassium phosphate as a base, provided the expected compound 49 [55]. Unfortunately, the yield remained very low (26%), but a preliminary attempt of C-C bond formation using Jepsen's conditions under microwave irradiation (PdCl 2 (PPh 3 ) 2 , K 2 CO 3 , DME/H 2 O, 80 • C, MW, 2 h) [52] proved to be unsuccessful with degradation of the reaction mixture.
Finally, the reaction of biaryl 49 with cesium carbonate at 150 • C in acetonitrile, under microwave irradiation, furnished compound 19, involving a fluorine atom as the leaving group for nucleophilic substitution. The NMR data and physicochemical properties of the compound obtained after ring closure reaction exactly matched with the previously described dibenzofuran derivative 19 in Scheme 2, confirming the proposed regioselectivity of the introduction of the carbamoyl function by CSI reagent. Nevertheless, after analyzing the global results of this synthetic route, optimization work will be necessary regarding the Suzuki-Miyaura coupling to consider it as a valuable approach of our series. Furthermore, when comparing the yield to obtain compound 19 in this reaction sequence (4.3% in 4 steps from compound 4) and the synthesis developed above (6.7% in 5 steps from compound 4, see Schemes 1 and 2), the second approach is less favored.
Taking into account the kinase inhibitory activity of cercosporamide (Tables 1 and 2, entry 1), the development of dibenzofuran derivatives led us to obtain more potent compounds against Pim-1/2 or as active compounds as the reference molecule. In addition, the novel compounds gave rise to low micromolar and nanomolar CLK1 inhibition compared to cercosporamide, constituting a very interesting result due to the CLK1 implications in human disease including cancer [57]. Taking into account the kinase inhibitory activity of cercosporamide (Tables 1 and 2, entry 1), the development of dibenzofuran derivatives led us to obtain more potent compounds against Pim-1/2 or as active compounds as the reference molecule. In addition, the novel compounds gave rise to low micromolar and nanomolar CLK1 inhibition compared to cercosporamide, constituting a very interesting result due to the CLK1 implications in human disease including cancer [57].

Docking Studies
Compounds 44, 43, and cercosporamide exhibit a similar binding mode when docked into the ATP pocket of Pim-1 (Figure 3). The 1-OH position in the benzamide portion forms a H-bond interaction with the carbonyl group of hinge residue Glu121 while the 7-OH position could favor an additional H-bond interaction with the ammonium side chain of conserved residue Lys67 and/or the carboxylate side chain of Asp186 (only visible for the most active compound 44). Such a binding mode is consistent with the experimental data, where 1,3-dihydroxybenzamide series (series B) are more much active than their 1,3-dimethoxy analogues (series A). All the 3-OH are oriented towards the carbonyl group of hinge residue Pro123, but the distance appears to be too large (>4.0 Å) to enable the formation of an appropriate H-bond interaction. The 4-carboxamide group does not seem to interact with a particular residue in the ATP-pocket but instead performs an intramolecular H-bond with the furan ring. This contrasts with previous docking studies of cercosporamide in the ATP pocket of Mnk2 kinase that showed H-bond interaction between the 4-carboxamide of the phenyl portion and hinge residues Glu160 and Met162 [8]. However, other docking studies of usnic acid, another dibenzofuran analogue with the main structural difference being 4-acetyl and 2-methyl groups, proposed a different binding mode with Pim-1 kinase, which seems consistent with our hypothesis [27]. Of course, we cannot discard other possible binding modes for these dibenzo[b,d]furan inhibitors since they contain many H-bond donor and acceptor substituents that can interact depending upon the specific environment of each kinase. The information of the role of the amide function in the binding mode of the compounds is under study through the synthesis of the counterparts without amide function. In a rational approach, their binding pose will be compared to their inhibitory potency on Pim-1 to complete the study. cannot discard other possible binding modes for these dibenzo[b,d]furan inhibitors since they contain many H-bond donor and acceptor substituents that can interact depending upon the specific environment of each kinase. The information of the role of the amide function in the binding mode of the compounds is under study through the synthesis of the counterparts without amide function. In a rational approach, their binding pose will be compared to their inhibitory potency on Pim-1 to complete the study.

In Vitro Cell-Based Assays and Acute Toxicity Testing
The Pim-1/2 and CLK-1 inhibitors 15-17 and 43-46, belonging to series B, were tested for their antiproliferative activity against six cancer cell lines, and L929 (mouse fibroblasts)

In Vitro Cell-Based Assays and Acute Toxicity Testing
The Pim-1/2 and CLK-1 inhibitors 15-17 and 43-46, belonging to series B, were tested for their antiproliferative activity against six cancer cell lines, and L929 (mouse fibroblasts) for in vitro toxicity evaluation (Table 3, entries 1 to 7). Cercosporamide as a reference and compound 47, being less active against Pim-1 and inactive on CLK1, were also considered in the anticancer screening (Table 3, entries 8 and 9). The commercially available compound SGI-1776, a small-molecule pan-Pim protein kinase inhibitor with IC 50 values of 0.05 (Pim-1) and 0.10 µM (Pim-2), was used as a positive control for the in vitro studies. Various types of hematological malignancies were selected for in vitro testing: the human acute myeloid leukemia (AML) cell line MV4-11, and the human chronic myeloid leukemia (CML) cell lines KU812 and K562. Indeed, Pim kinases are overexpressed in a wide range of hematopoietic malignancies [27][28][29][30][31][32][33][34][35][36]. Solid tumors were also included in the panel of tested cell lines: MCF-7 (human breast adenocarcinoma), HT-29 (human colon adenocarcinoma), and HeLa (human cervical cancer) cells. Cytotoxic effects were evaluated using an MTT assay, and living cells were also counted with the trypan blue dye exclusion method for MV4-11, KU812, and K562 cells. acute myeloid leukemia (AML) cell line MV4-11, and the human chronic myeloid leukemia (CML) cell lines KU812 and K562. Indeed, Pim kinases are overexpressed in a wide range of hematopoietic malignancies [27][28][29][30][31][32][33][34][35][36]. Solid tumors were also included in the panel of tested cell lines: MCF-7 (human breast adenocarcinoma), HT-29 (human colon adenocarcinoma), and HeLa (human cervical cancer) cells. Cytotoxic effects were evaluated using an MTT assay, and living cells were also counted with the trypan blue dye exclusion method for MV4-11, KU812, and K562 cells.  In a general trend, all the dibenzofuran derivatives, except 47 (Table 3, entry 8), displayed moderate to good antiproliferative activity against the AML cell line MV4-11, with IC50 values ranging from 2.6 ± 0.4 to 52.2 ± 1.7 µM (Table 3, entries 1-7). Compound 44 was the most active compound but remained nearly 90-fold less active than the reference drug SGI-1776 on this cell (Table 3,   In a general trend, all the dibenzofuran derivatives, except 47 (Table 3, entry 8), displayed moderate to good antiproliferative activity against the AML cell line MV4-11, with IC 50 values ranging from 2.6 ± 0.4 to 52.2 ± 1.7 µM (Table 3, entries 1-7). Compound 44 was the most active compound but remained nearly 90-fold less active than the reference drug SGI-1776 on this cell (Table 3, (Table 1, entries 15, 9, and 3). Moreover, the inactive compound 47 against the MV4-11 cell lines displayed a higher IC 50 value of 2.35 µM against Pim-1 and was also inactive towards CLK1 kinase. It must be noticed that the majority of dihydroxydibenzofuran derivatives designed in the frame of that project showed better activity than cercosporamide (IC 50 = 31.5 ± 4.7 µM) against MV4-11 cells.
Regarding the antileukemia effect of the compounds, it is noteworthy that disappointing pharmacological results against CML cell lines, namely KU812 and K562, suggested a selective effect on AML cell growth (Table 3). Considering the other types of tumors, all the tested compounds remained totally inactive against the MCF-7 cell line, except 44 (IC 50 = 52.5 ± 1.2 µM) with moderate potency. In the contrary, HeLa cells proved to be very sensitive to part of the synthesized compounds, with IC 50 values ranging from 9.5 ± 4.1 to 24.7 ± 6.7 µM, for the most active compounds and at the level of cercosporamide (Table 3, entry 9) for analogues 44 and 17 (Table 3, entries 5 and 3).
Against L929 normal cells, no cytotoxicity (Table 3, entries 2-4 and 6) or poor cytotoxicity (Table 3, entries 1 and 5) was observed, strengthening the interest in the series in terms of the selectivity index. Complementing this study, the determination of in vivo toxicity, using Galleria mellonella larvae [22], was performed for all the compounds involved in the antitumor assays. Indeed, Galleria mellonella has been demonstrated to be a more robust assessment of the likely toxicity of chemicals in mammals than cell lines [58]. Groups of 10 larvae were injected with 10 µL of compound at a dose varying from 10 to 50 mg/kg. The mortality was recorded daily for 7 days. Percentage survival was plotted for each compound using Graph Pad Prism. No reduction of viability and no sign of cuticular darkening were observed for any compounds, except for 16 and 45, at a dose of 50 mg/kg after 7 days (Figure 4). Survival analysis was done using the log-rank test and the Kaplan-Meier survival curves for these two compounds. Concerning 16, the 2 tests showed non-significant p-values (0.1464 and 0.1468, respectively) and allowed the conclusion that the survival curve was not significantly different to the other compounds. Concerning 45, the same conclusions were obtained with p-values equal to 0.3173 for the two tests. These encouraging results prompted us to conclude to a general nontoxic effect in vivo in this model and at the considered dose. cept 44 (IC50 = 52.5 ± 1.2 µM) with moderate potency. In the contrary, HeLa cells proved to be very sensitive to part of the synthesized compounds, with IC50 values ranging from 9.5 ± 4.1 to 24.7 ± 6.7 µM, for the most active compounds and at the level of cercosporamide (Table 3, entry 9) for analogues 44 and 17 (Table 3, entries 5 and 3).
Against L929 normal cells, no cytotoxicity (Table 3, entries 2-4 and 6) or poor cytotoxicity (Table 3, entries 1 and 5) was observed, strengthening the interest in the series in terms of the selectivity index. Complementing this study, the determination of in vivo toxicity, using Galleria mellonella larvae [22], was performed for all the compounds involved in the antitumor assays. Indeed, Galleria mellonella has been demonstrated to be a more robust assessment of the likely toxicity of chemicals in mammals than cell lines [58]. Groups of 10 larvae were injected with 10 µL of compound at a dose varying from 10 to 50 mg/kg. The mortality was recorded daily for 7 days. Percentage survival was plotted for each compound using Graph Pad Prism. No reduction of viability and no sign of cuticular darkening were observed for any compounds, except for 16 and 45, at a dose of 50 mg/kg after 7 days (Figure 4). Survival analysis was done using the log-rank test and the Kaplan-Meier survival curves for these two compounds. Concerning 16, the 2 tests showed non-significant p-values (0.1464 and 0.1468, respectively) and allowed the conclusion that the survival curve was not significantly different to the other compounds. Concerning 45, the same conclusions were obtained with p-values equal to 0.3173 for the two tests. These encouraging results prompted us to conclude to a general nontoxic effect in vivo in this model and at the considered dose.

General Experimental Procedures
All commercial reagents were used without further purification. All solvents were reagent or HPLC grade. Analytical TLC was performed on silica gel 60 F254 plates. Open column chromatography was performed on silica gel 60 (70-230 mesh ASTM, Macherey-Nagel GmbH & Co. KG, Düren, Germany). The flash column chromatography was performed using a Reveleris ® X2 Buchi system, with a Buchi cartridge. Yields refer to chromatographically and spectroscopically pure compounds. Melting points were determined on an electrothermal melting point (Thermo Fisher Scientific, Illkirch, France) apparatus. 1 H NMR and 13 C NMR spectra were recorded in CDCl 3 or in DMSO-d 6 on a 400 MHz spectrometer. Chemical shifts are reported as δ values in parts per million (ppm) relative to tetramethylsilane as the internal standard and coupling constants (J) are given in hertz. Multiplicities are reported as follows: s = singlet, d = doublet, dd = doublet of doublets, t = triplet, mt = multiplet, bs = broad singlet, t app = apparent triplet. All the spectra are provided in the "Supplementary Materials". Low-resolution mass spectra were recorded using an electrospray ionization (ESI) method with a Waters ZQ 2000 spectrometer (Waters, Saint Quentin en Yvelines, France). The UPLC column used was an Acquity UPLC ® BEH Phenyl (2.1 mm i.d., 50 mm length, 1.7 µm particle size, Waters, Saint Quentin en Yvelines, France) from Waters. A linear mobile phase gradient was used with mobile phase A as 100% of acetonitrile in water (at 2%) and mobile phase B as 100% acetonitrile. The gradient table was: 0-0.5 min, 0% B; 0.5-4.0 min 0→100% B; 4.0-5.5% 100% B; 5.5-5.7 min 100→0% B; 5.7-7.5 min 0% B at a flow rate of 0.5 mL·min −1 and column temperature of 35 • C. Formic acid (0.1%) was added in diluent to improve ionization. For compounds 14-19 and 38-47, high-resolution mass spectrometry (HRMS) was recorded on a Waters Vion IMS QTof instrument (SAA055K, Waters, Manchester, UK) coupled with an Acquity H-Class UPLC in ESI+ mode. IR absorption spectra were recorded on an ATR-FTIR equipment, MIRacle Shimadzu spectrometer (Shimadzu Corporation, Kyoto, Japan). Only the most significant bands were reported. Microwave reactions were carried out in a CEM Discover microwave reactor (CEM SAS, Saclay, France) in sealed vessels (monowave, maximum power 300 W, temperature control via IR-sensor, fixed temperature).

Access to Dibenzofuran Derivatives 14-19 by Intramolecular C-C Bond Formation from Diaryl Ethers 2-Hydroxy-4,6-dimethoxybenzamide (2)
To a stirred suspension of 3,5-dimethoxyphenol 4 (1.00 g, 6.5 mmol) in acetonitrile (20 mL) at 0 • C under argon, chlorosulfonyl isocyanate (1.26 mL, 13 mmol) was added. The mixture was maintained at this temperature for 10 min and then the reaction mixture was quenched with HCl 5 M (20 mL). After 10 h at room temperature, the reaction was diluted with water (100 mL) and extracted with dichloromethane (3 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (8/2) as eluent to give compound 2 (576 mg, 45% yield) as a white powder. 2,4-Dimethoxy-6-(2 -nitrophenoxy)benzamide (6) A mixture of 2-hydroxy-4,6-dimethoxybenzamide 2 (0.50 g, 2.5 mmol) and potassium hydroxide (0.13 g, 2.5 mmol) was heated to 120 • C and stirred for 30 min, and then 1-iodo-2-nitrobenzene (0.64 g, 2.5 mmol) and copper powder (0.20 g, 3.13 mmol) were added, respectively. The resulting mixture was stirred at 170 • C for 24 h and then cooled down to room temperature. After the addition of water, the aqueous layer was extracted with ethyl acetate (100 mL) and washed with water (2 × 100 mL). The organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated to dryness. The resulting oil was purified by silica gel column chromatography using cyclohexane/ethyl acetate (8/2) as an eluent to give compound 6 (125 mg, 15% yield) as a brown powder.

1,3-Dibenzyloxy-5-(2 -nitrophenoxy)benzene (7)
A mixture of 3,5-dibenzyloxyphenol 3 (1.00 g, 3.26 mmol) and potassium hydroxide (0.18 g, 3.26 mmol) was heated to 120 • C and stirred for 30 min, and then 1-iodo-2nitrobenzene (0.82 g, 3.26 mmol) and copper powder (0.40 g, 6.25 mmol) were added, respectively. The resulting mixture was stirred at 170 • C for 2 h and then cooled down to room temperature. After the addition of water, the aqueous layer was extracted with ethyl acetate (100 mL) and washed with water (2 × 100 mL). The organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated to dryness. The resulting oil was purified by silica gel column chromatography using cyclohexane/ethyl acetate (8/2) as the eluent to give compound 7 (1.02 g, 73% yield) as a yellow powder.  (8) A mixture of 3,5-dimethoxyphenol 4 (1.00 g, 6.5 mmol) and potassium hydroxide (0.18 g, 6.5 mmol) was heated to 120 • C and stirred for 30 min, and then 1-iodo-2nitrobenzene (0.82 g, 6.5 mmol) and copper powder (1.00 g, 15.6 mmol) were added, respectively. The resulting mixture was stirred at 170 • C for 2 h and then cooled down to room temperature. After the addition of water, the aqueous layer was extracted with ethyl acetate (100 mL). The organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated to dryness. The resulting oil was purified by silica gel column chromatography using cyclohexane/ethyl acetate (8/2) as eluent to give compound 8 (1.4 g 78% yield) as a yellow oil. To a solution of 1,3-dibenzyloxy-5-(2 -nitrophenoxy)benzene 7 (100 mg, 0.23 mmol) in methanol (10 mL) at room temperature, saturated aqueous ammonium chloride (5 mL) and zinc dust (75 mg, 1.15 mmol) were added sequentially. After stirring for 30 min at room temperature, additional zinc was added (75 mg, 1.15 mmol) and the reaction mixture was refluxed for 2 h. After the addition of water, the aqueous layer was extracted with dichloromethane (3 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (8/2) as the eluent to give compound 9 (59 mg, 65% yield) as a brown powder.

2-(3 ,5 -Dimethoxyphenoxy)aniline (10)
To a solution of 1,3-dimethoxy-5-(2 -nitrophenoxy)benzene 8 (250 mg, 0.91 mmol) in methanol (10 mL) at room temperature, saturated aqueous ammonium chloride (5 mL) and zinc dust (295 mg, 4.54 mmol) were added sequentially. After stirring for 30 min at room temperature, additional zinc was added (295 mg, 4.54 mmol) and the reaction mixture was refluxed for 2 h. After the addition of water, the aqueous layer was extracted with ethyl acetate (100 mL) and washed with water (2 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 7 (205 mg, 92% yield) as a brown oil.  (13) 1,3-Dimethoxy-5-(2 -nitrophenoxy)benzene 8 (1.00 g, 3.6 mmol) was dissolved in warm pivalic acid (50 • C, 60 g). Silver acetate (1.20 g, 7.2 mmol) and palladium (II) acetate (40 mg, 0.18 mmol) were added, and the reaction mixture was properly stirred at 130 • C for 4 h. After cooling to room temperature and the addition of water (100 mL), the aqueous layer was extracted with ethyl acetate (100 mL). The organic layers were washed with brine, dried over sodium sulfate, filtered, and evaporated to dryness. The resulting oil was purified by silica gel column chromatography using cyclohexane/ethyl acetate (8/2) as the eluent to give compound 13 (0.71 g, 72% yield) as a yellow powder. To a stirred suspension of 1,3-dimethoxy-6-nitrodibenzo[b,d]furan 13 (1.00 g, 3.7 mmol) in acetonitrile (20 mL) at 0 • C under argon, chlorosulfonyl isocyanate (0.64 mL, 7.4 mmol) was added. The mixture was maintained at room temperature for 12 h and then the reaction mixture was quenched with HCl 5 M (20 mL). After 6 h at room temperature, the reaction was diluted with water (100 mL) and extracted with ethyl acetate (100 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 14 (0.61 g, 53% yield) as a yellow powder.

1,3-Dihydroxy-6-nitrodibenzo[b,d]furan-4-carboxamide (15)
A mixture of 1,3-dimethoxy-6-nitrodibenzo[b,d]furan-4-carboxamide 14 (50 mg, 0.16 mmol) in pyridine hydrochloride (3g) was irradiated using microwave heating at 200 • C for 15 min. After the addition of water, the aqueous layer was extracted with ethyl acetate (50 mL) and the resulting organic layer was washed with water (2 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 15 (23 mg, 51% yield) as a yellow powder. R f 0.67 (CH 2 Cl 2 /MeOH 9:1); Mp > 300 • C; 1  in methanol (10 mL) at room temperature, saturated aqueous ammonium chloride (5 mL) and zinc dust (55 mg, 0.85 mmol) were added sequentially. After stirring for 30 min at room temperature, additional zinc was added (55 mg, 0.85 mmol) and the reaction mixture was refluxed for 2 h. After the addition of water, the aqueous layer was extracted with ethyl acetate (100 mL) and the resulting organic layer was washed with water (2 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 16 (28 mg, 62% yield) as a brown powder. Afterwards, the reaction mixture was stirred for 45 min at 80 • C and then the reaction was quenched with water. The aqueous layer was extracted with ethyl acetate (100 mL) and the organic layer was washed with brine, dried over sodium sulfate, and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 17 (49 mg, 52% yield) as a brown powder.
Method B: A mixture of 1,3-dimethoxydibenzo[b,d]furan-4-carboxamide 19 (see below) (100 mg, 0.37 mmol) in pyridine hydrochloride (3 g) was irradiated using microwave heating at 200 • C for 15 min. After the addition of water, the aqueous layer was extracted with ethyl acetate (50 mL) and the resulting organic layer was washed with water (2 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 17 (43 mg, 48% yield) as a brown powder. in methanol (10 mL) at room temperature, saturated aqueous ammonium chloride (5 mL) and zinc dust (55 mg, 0.85 mmol) were added sequentially. After stirring for 30 min at room temperature, additional zinc was added (55 mg, 0.85 mmol) and the reaction mixture was refluxed for 2 h. After the addition of water, the aqueous layer was extracted with ethyl acetate (100 mL) and the resulting organic layer was washed with water (2 × 50 mL). The combined organic extracts were dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as eluent to give compound 18 ( The reaction mixture was stirred for 45 min at 80 • C and then the reaction was quenched with water. The aqueous layer was extracted with ethyl acetate (100 mL) and the organic layer was washed with brine, dried over sodium sulfate, and filtered. The solvent was evaporated in vacuo and the residue was purified by silica gel column chromatography using cyclohexane/ethyl acetate (6/4) as the eluent to give compound 19 (22 mg, 46% yield) as a pale brown powder.
Method B: A mixture of 2'-fluoro-2-hydroxy-4,6-dimethoxybiphenyl-3-carboxamide 49 (see below) (50 mg, 0.17 mmol) and cesium carbonate (166 mg, 0.51 mmol) in acetonitrile was irradiated using microwave (80 W) heating at 150 • C for 1 h. Ethyl acetate (50 mL) was added to the mixture and the organic layer was washed with water (2 × 50 mL), dried over sodium sulfate, and filtered. The solvent was evaporated in vacuo and the residue was crystallized from diisopropyl ether to furnish the title compound 19 as a pale brown solid ( Access to dibenzofuran derivatives 38-47 was achieved by intramolecular C-C bond formation from diaryl ethers.
The cancer cell lines MCF-7, HT-29, and HeLa were obtained from Cell Bank of Rio de Janeiro, Brazil, and were grown in RPMI-1640 medium and L929 cells were grown in Dulbecco's Modified Eagle's Medium (DMEM). Both media were supplemented with fetal bovine serum (FBS) (10% of the final concentration) followed by treatment with penicillin and streptomycin as antibiotics (1% of the final concentration). The crude was incubated in the presence of CO 2 atmosphere (5%) for 24 h with the temperature monitored and maintained at 37 • C.

In Vitro Cell-Based Assays
MV4-11, K562, and KU812 cells' viability was studied using a MTT cell proliferation assay. To determine the concentration effect of the molecules, 0.2 × 10 6 leukemic cells were incubated in 100 µL of RPMI red phenol-free medium (Gibco), in 96-well plates and treated with compounds 15-17 and 43-47 (stock solution at 50 mM in DMSO) or the positive control SGI-1776 from SAB Signalway antibody with concentrations ranging from 100 nM to 100 µM for 48 h.
MV4-11, K562, and KU812 cells were incubated with 10 µL of MTT working solution (5 g/L of methylthiazolyldiphenyl-tetrazolium bromide from Sigma Aldrich, Lyon, France) for 4 h. Cells were then lysed overnight at 37 • C with 100 µL of 10% SDS and 0.003% HCl. Optical density (OD) at 570 nm was measured using a spectrophotometer CLARIOstar ® (BMG Labtech, Offenburg, Germany). Living cells were also counted with the trypan blue dye exclusion method. When a dose-dependent activity was observed, IC 50 values were calculated using Graphpad PRISM 7 software (n = 3 in triplicate). Data were collected from at least three independent experiments and the values reported are means ± standard errors of the mean (SEM). MCF-7, HT-29, HeLa, and L929 cells (3×10 5 cells/mL) were plated in 96-well plates and incubated in the presence of CO 2 atmosphere (5%) for 24 h at 37 • C. Then, compounds 15-17 and 43-47 with concentrations ranging from 100 nM to 100 µM were added and incubated at 37 • C for 72 h. Then, 25 µL of MTT (5 mg/mL in PBS) were added to each well and the plates were left for another 3 h in the incubator at 37 • C and at the end of this period, the supernatant was aspirated. To perform the reading, 100 µL of dimethylsulfoxide (DMSO) were added in each well for the dissolution of the formazan crystals. The amount of formazan was measured by reading the plate at 560 nm absorbance. The concentration leading to 50% inhibition of viability (IC 50 ) was calculated by regression analysis using GraphPad Prism 5.0. Each sample was tested in triplicate in two independent experiments. Doxorubicin was used as a positive control.

Evaluation of Compounds' Cytotoxicity Using the In Vivo Galleria mellonella Model
G. mellonella larvae were bred in the IICiMed laboratory at 30-32 • C in a mixture of flour, honey, oatmeal, glycerol, and pollen until 260 to 320 mg (6 th developmental stage). Groups of 10 larvae were randomly selected for the experiments. Each larva was injected in the 4 th left pro-leg with 10 µL of compounds or an equivalent volume of PBS/DMSO for the control group at a dose varying between 10 and 50 mg/kg using a 0.3 mL-30G-Insulin syringe. Larvae were incubated in the dark at 37 • C and survival was evaluated daily for 7 days. Death was assessed by the lack of movement in response to stimulus, with or without discoloration. Percentage survival was plotted using GraphPad Prism and survival analyses were determined using the log-rank test and the Kaplan-Meier survival curves. Two independent experiments were performed.

Conclusions
In summary, our research efforts in the synthesis of a natural product-inspired chemotype, endowed with kinase inhibition properties, produced attractive and safe 1,3-dihydroxydibenzo[b,d]furan derivatives. This study confirmed cercosporamide as a valuable starting point for medicinal chemistry investigations and the removal of chirality afforded a flat tricyclic core with very promising enzyme and cellular potencies. Indeed, it was well established that the dual Pim1/2-CLK kinases inhibition promoted very interesting antiproliferative properties against the AML cell line as confirmed by the growth inhibitory activity at a low micromolar concentration, coupled with the nanomolar IC 50 values obtained against the enzymes, particularly for the most active compound 44.
Docking studies demonstrated that the 4-carboxamide group of the dibenzofurans was not essential for binding within the ATP pocket of Pim-1, in contrast to the hydroxy groups. In future, the replacement of the carboxamide group will be investigated to validate the hypothesis, and taking into account the level of activity of usnic acid towards Pim1/2, its substitution pattern could also serve as model for further design. In parallel, the very encouraging results obtained for this synthetic compound library deserve further optimization for complete SAR studies.