A “Pretender” Croconate-Bridged Macrocyclic Tetraruthenium Complex: Sizable Redox Potential Splittings despite Electronically Insulated Divinylphenylene Diruthenium Entities

Careful optimization of the reaction conditions provided access to the particularly small tetraruthenium macrocycle 2Ru2Ph-Croc, which is composed out of two redox-active divinylphenylene-bridged diruthenium entities {Ru}-1,4-CH=CH-C6H4-CH=CH-{Ru} (Ru2Ph; {Ru} = Ru(CO)Cl(PiPr3)2) and two likewise redox-active and potentially non-innocent croconate linkers. According to single X-ray diffraction analysis, the central cavity of 2Ru2Ph-Croc is shielded by the bulky PiPr3 ligands, which come into close contact. Cyclic voltammetry revealed two pairs of split anodic waves in the weakly ion pairing CH2Cl2/NBu4BArF24 (BArF24 = [B{C6H3(CF3)2-3,5}4]− electrolyte, while the third and fourth waves fall together in CH2Cl2/NBu4PF6. The various oxidized forms were electrogenerated and scrutinized by IR and UV/Vis/NIR spectroscopy. This allowed us to assign the individual oxidations to the metal-organic Ru2Ph entities within 2Ru2Ph-Croc, while the croconate ligands remain largely uninvolved. The lack of specific NIR bands that could be assigned to intervalence charge transfer (IVCT) in the mono- and trications indicates that these mixed-valent species are strictly charge-localized. 2Ru2Ph-Croc is hence an exemplary case, where stepwise IR band shifts and quite sizable redox splittings between consecutive one-electron oxidations would, on first sight, point to electronic coupling, but are exclusively due to electrostatic and inductive effects. This makes 2Ru2Ph-Croc a true “pretender”.


Introduction
Their inherent symmetry and the relative ease of their fabrication by the self-assembly of mononuclear metal-coligand nodes or dinuclear clamps and ditopic organic linkers as well as their structural adaptability render metallamacrocycles a particularly appealing class of compounds [1][2][3][4][5][6][7]. Besides their sheer beauty, their ability to undergo redox processes as encoded by the their metal constituents or the use of redox-active linkers has led to interesting applications that range from the redox-triggered release of incorporated guest molecules [8][9][10] to their use as superior water oxidation catalysts [11][12][13][14]. In our search of π-conjugated bis(β-ketoenolate) ligands with an angular arrangement of the chelating functionalities, we identified the croconate dianion C5O5 2− as a particularly promising candidate. Croconate belongs to the compound class of oxycarbons with the general formula CnOn m− and was first synthesized as early as in 1825 [42]. Its name derives from the yellow color of its metal salts, which is highly reminiscent of some variants of the crocus flower and of egg yolk. With its planar structure, its (nearly) perfect fivefold symmetry, even in the crystalline state [43][44][45], and its two delocalized π-electrons, the croconate dianion meets the formal criteria of a Hückel arene ( Figure 2). Quantum chemical studies however indicate that its degree of aromaticity is considerably lower than that of ordinary arenes [44,46]. Moreover, the croconate dianion undergoes two reversible one-electron oxidations at similarly low potentials as divinylarylene-bridged diruthenium complexes [23,[47][48][49][50][51][52] (e.g., E1/2 2−/− = −240/−310 mV, E1/2 −/0 = 5/100 mV against the ferrocene/ferrocenium redox couple FcH/FcH + in DMF as the solvent, depending on the supporting electrolyte) [53,54]. Electroactivity is usually also maintained on complexation with concomitant shifts to more anodic potentials as a result of electron donation to the metal ions [55].   In our search of π-conjugated bis(β-ketoenolate) ligands with an angular arrangement of the chelating functionalities, we identified the croconate dianion C5O5 2− as a particularly promising candidate. Croconate belongs to the compound class of oxycarbons with the general formula CnOn m− and was first synthesized as early as in 1825 [42]. Its name derives from the yellow color of its metal salts, which is highly reminiscent of some variants of the crocus flower and of egg yolk. With its planar structure, its (nearly) perfect fivefold symmetry, even in the crystalline state [43][44][45], and its two delocalized π-electrons, the croconate dianion meets the formal criteria of a Hückel arene ( Figure 2). Quantum chemical studies however indicate that its degree of aromaticity is considerably lower than that of ordinary arenes [44,46]. Moreover, the croconate dianion undergoes two reversible one-electron oxidations at similarly low potentials as divinylarylene-bridged diruthenium complexes [23,[47][48][49][50][51][52] (e.g., E1/2 2−/− = −240/−310 mV, E1/2 −/0 = 5/100 mV against the ferrocene/ferrocenium redox couple FcH/FcH + in DMF as the solvent, depending on the supporting electrolyte) [53,54]. Electroactivity is usually also maintained on complexation with concomitant shifts to more anodic potentials as a result of electron donation to the metal ions [55]. Among the many coordination modes that this highly adaptive ligand may exhibit in transition metal complexes [56,57], the µ,κ 2 -O 1 ,O 2 ,κ 2 -O 3 ,O 4 -binding mode (Figure 2), which has been observed on several occasions [56][57][58][59][60][61][62], would be well-suited to forge divinylphenylene-bridged diruthenium building blocks {Ru(CO)(P i Pr3)2}2(1,4-CH=CH-C6H4- Among the many coordination modes that this highly adaptive ligand may exhibit in transition metal complexes [56,57], the µ,κ 2 -O 1 ,O 2 ,κ 2 -O 3 ,O 4 -binding mode (Figure 2), which has been observed on several occasions [56][57][58][59][60][61][62], would be well-suited to forge divinylphenylene-bridged diruthenium building blocks {Ru(CO)(P i Pr 3 ) 2 } 2 (1,4-CH=CH-C 6 H 4 -CH=CH), henceforth abbreviated as Ru 2 Ph, into macrocyclic structures. In addition, croconate also offers the degree of coordinative lability and flexibility of its coordination modes required to transform initially formed mixtures of different di-or oligomeric complexes into the thermodynamically preferred macrocycle(s) [30,31,[63][64][65][66][67]. Here, we report on the successful isolation and in-depth characterization of a tetranuclear macrocyclic complex with considerably smaller dimensions as previous dicarboxylate-bridged congeners. Of particular relevance is the observation of three or even four individually resolved one-Molecules 2021, 26, 5232 4 of 25 electron oxidations. The issues of the loci of the anodic processes and of the presence or absence of electronic coupling in the mixed-valent states are addressed by a multimethod approach, including IR, UV/Vis/NIR, and EPR spectroscopy with support by quantum chemistry.

Synthesis, Spectroscopic Identification, and X-ray Crystallography
The insolubility of alkali metal salts of croconic acid in methanol called for the use of biphasic mixtures consisting of a solution of the 1,4-divinylphenylene-bridged diruthenium precursor {Ru(CO)Cl(P i Pr 3 ) 2 } 2 (1,4-CH=CH-C 6 H 4 -CH=CH) (Ru 2 Ph-Cl) [47] in either CH 2 Cl 2 or benzene, an aqueous solution of a slight excess (1.06 equivalents) of potassium croconate monohydrate [68,69], and a few drops of methanol as a phase transfer agent for synthesis. NMR spectra obtained from the solid product obtained after appropriate workup (see the Experimental Section) indicated the presence of two different species as revealed by the two sharp singlet resonances in the 31 P NMR spectrum and two sets of resonances for the Ru-CH=CH and the Ru-CH=CH protons with integration ratios of ca. 3:2 (Scheme 1 and Figures S1 and S2 of the Supplementary Materials). This pattern is suggestive of two kinds of macrocyclic complexes that differ in their nuclearities, similar to what we have observed on previous occasions [27,30,31]. High-resolution mass spectro-metry indeed provides a molar peak at 2336.78 g mol −1 (calcd. 2336.77) which comprises of two different envelopes, one giving rise to an isotopic pattern with spacings by one mass unit, and one with a half-integer spacing ( Figure 3). The observed pattern thus corresponds to the molecule ion peak of a tetranuclear species 2 Ru 2 Ph-Croc that consists of two equivalents of each building block, and that of the doubly charged octanuclear complex 4 Ru 2 Ph-Croc comprising of four equivalents of each constituent. Putative structures of these supramolecular isomers are shown in Scheme 1. They most likely differ with respect to the orientation of the croconate ligand, i.e., by whether the non-coordinating carbonyl functionality is oriented outwards ( 2 Ru 2 Ph-Croc) or towards the interior ( 4 Ru 2 Ph-Croc) of the macrocyclic cavity and by the ensuing angles of the Ru-C 5 O 5,centre -Ru vector of ca. 72 • or 144 • as dictated by the fivefold symmetry of the croconate dianion. All attempts to separate the two differently sized macrocycles through either enrichment by repeated washings, fractionated crystallization or selective precipitation remained futile. In consequence, the reaction conditions had to be optimized in such a way as to favor the formation of one kind of macrocycle over the other (note that chromatographic separation is not possible to the sensitivity of the Ru-vinyl linkage to any Brønsted or Lewis acid).
Through an iterative process of varying reaction time, temperature, and solvent we were able to identify conditions that afforded pure 2 Ru 2 Ph-Croc, namely: (i) using a saturated (27 mM) solution of Ru 2 Ph-Cl in benzene as the tetranuclear macrocycle is less soluble in this solvent than the octanuclear congener and hence precipitates first; (ii) conducting the reaction at a slightly elevated reaction temperature of 35 • C; and (iii) limiting the reaction time to 19 h, as the smaller macrocycle constitutes the kinetic product. Shorter reaction times led to incomplete conversion of difficult-to-separate Ru 2 Ph-Cl, while longer reaction times lead to increasing amounts of the larger macrocycle. 2 Ru 2 Ph-Croc could be ultimately obtained in a yield of 21%, which is only modest when compared to what can be achieved for similar dicarboxylate-linked macrocycles [25,[28][29][30][31][32]. Underlying reasons are that a significant portion of the tetranuclear macrocycle remains in solution and cannot be separated from slightly better soluble 4 Ru 2 Ph-Croc and the difficulties encountered when separating the precipitated solid from the biphasic supernatant.  Through an iterative process of varying reaction time, temperature, and solvent we were able to identify conditions that afforded pure 2 Ru2Ph-Croc, namely: (i) using a saturated (27 mM) solution of Ru2Ph-Cl in benzene as the tetranuclear macrocycle is less soluble in this solvent than the octanuclear congener and hence precipitates first; (ii) conducting the reaction at a slightly elevated reaction temperature of 35 °C; and (iii) limiting the reaction time to 19 h, as the smaller macrocycle constitutes the kinetic product. Shorter reaction times led to incomplete conversion of difficult-to-separate Ru2Ph-Cl, while longer reaction times lead to increasing amounts of the larger macrocycle. 2 Ru2Ph-Croc could be ultimately obtained in a yield of 21%, which is only modest when compared to what can be achieved for similar dicarboxylate-linked macrocycles [25,[28][29][30][31][32]. Underlying reasons are that a significant portion of the tetranuclear macrocycle remains in solution and cannot be separated from slightly better soluble 4 Ru2Ph-Croc and the difficulties encountered when separating the precipitated solid from the biphasic supernatant.  Through an iterative process of varying reaction time, temperature, and sol were able to identify conditions that afforded pure 2 Ru2Ph-Croc, namely: (i) usin rated (27 mM) solution of Ru2Ph-Cl in benzene as the tetranuclear macrocycle is uble in this solvent than the octanuclear congener and hence precipitates first; ducting the reaction at a slightly elevated reaction temperature of 35 °C; and (iii) the reaction time to 19 h, as the smaller macrocycle constitutes the kinetic product reaction times led to incomplete conversion of difficult-to-separate Ru2Ph-C longer reaction times lead to increasing amounts of the larger macrocycle. 2 Ru2 could be ultimately obtained in a yield of 21%, which is only modest when com what can be achieved for similar dicarboxylate-linked macrocycles [25,[28][29][30][31][32]. Un reasons are that a significant portion of the tetranuclear macrocycle remains in and cannot be separated from slightly better soluble 4 Ru2Ph-Croc and the difficu The high purity of isolated 2 Ru 2 Ph-Croc is evident from the 1 H-, 13 C-, and 31 P-NMR spectra (see Figure 4 and S3-S5 of the Supplementary Materials) as well as by mass spectrometry (see Figure S6 of the Supplementary Materials). The simplicity of the NMR patterns pay witness to the high symmetry of 2 Ru 2 Ph-Croc, with only one doublet resonance for each of the vinylic Ru-CH and the Ru-CH=CH protons at 9.29 and 6.21 ppm, respectively ( 3 J HH = 15.7 Hz), one singlet resonance for the phenylene protons at 7.15 ppm, and the expected resonances for the CH and the CH(CH 3 ) 2 protons of the P i Pr 3 ligands at 2.30 (CH(CH 3 ) 2 ) or 1.37 and 1.15 ppm for the diastereotopic methyl groups. Of note is the shift of the Ru-CH resonance to unusually low field as compared to ca. 8.5 ppm in dicarboxylate-bridged tetraruthenium macrocycles and to 8.87 ppm in the larger 4 Ru 2 Ph-Croc. This points to a large torsion of the Ru-CH=CH entities with respect to the plane of the phenylene linker and a concomitantly smaller degree of π-conjugation. The reduction of the symmetry of the bridging C 5 O 5 2− ligand from fivefold rotational symmetry to only a mirror plane through the uncoordinated keto function and the midpoint of the opposite C-C bond is mirrored by the observation of three distinct 13 C NMR resonances at 199.1, 184.8, and 180.6 ppm for the ring carbon atoms. tively ( 3 JHH = 15.7 Hz), one singlet resonance for the phenylene protons at 7.15 ppm, an the expected resonances for the CH and the CH(CH3)2 protons of the P i Pr3 ligands at 2. (CH(CH3)2) or 1.37 and 1.15 ppm for the diastereotopic methyl groups. Of note is the sh of the Ru-CH resonance to unusually low field as compared to ca. 8.5 ppm in dicarbo ylate-bridged tetraruthenium macrocycles and to 8.87 ppm in the larger 4 Ru2Ph-Croc. Th points to a large torsion of the Ru-CH=CH entities with respect to the plane of the ph nylene linker and a concomitantly smaller degree of π-conjugation. The reduction of th symmetry of the bridging C5O5 2− ligand from fivefold rotational symmetry to only a mirr plane through the uncoordinated keto function and the midpoint of the opposite C bond is mirrored by the observation of three distinct 13 C NMR resonances at 199.1, 184 and 180.6 ppm for the ring carbon atoms. Slow diffusion of methanol into a dichloromethane solution containing 2 Ru2Ph-Cr yielded a small crop of crystals that proved suitable for single crystal X-ray crystall graphy. Crystallographic details and a list of bond lengths and angles are provided in th Supplementary Materials (Tables S1-S3 and Figure S8). Figure 5 displays the molecul structure. Slow diffusion of methanol into a dichloromethane solution containing 2 Ru 2 Ph-Croc yielded a small crop of crystals that proved suitable for single crystal X-ray crystallography. Crystallographic details and a list of bond lengths and angles are provided in the Supplementary Materials (Tables S1-S3 and Figure S8). Figure 5 displays the molecular structure.  2 Ru2Ph-Croc crystallized in the monoclinic space group P121/n1 along with two CH2Cl2 and one MeOH solvate molecules per formula unit. The latter are disordered over three (CH2Cl2) or four sites (MeOH) and were treated by the SQUEEZE implementation [70] of OLEX [71]. The structure is inversion symmetric, rendering diagonally disposed vinyl ruthenium fragments equivalent. Assuming that the maximum accessible cavity has the shape of an octagon spanned by the coordinated oxygen atoms of the croconate ligand pointing inside the void and the inwards orientated hydrogen atoms on the phenylene three (CH 2 Cl 2 ) or four sites (MeOH) and were treated by the SQUEEZE implementation [70] of OLEX [71]. The structure is inversion symmetric, rendering diagonally disposed vinyl ruthenium fragments equivalent. Assuming that the maximum accessible cavity has the shape of an octagon spanned by the coordinated oxygen atoms of the croconate ligand pointing inside the void and the inwards orientated hydrogen atoms on the phenylene linkers, one obtains an area of 55.2 Å 2 with dimensions of 9.87 Å × 7.00 Å for its long (O···O distance) and short (H···H distance) axis. This equals to just about 50% to 60% of the size of the rectangular cavity in the benzoate-bridged analog [{Ru(CO)(P i Pr 3 ) 2 } 2 (µ-1,4-CH=CH-C 6 H 4 -CH=CH)] 2 {1,3-(OOC) 2 -C 6 H 3 -5-SAcyl} 2 [29]. The decreased dimensions of the void are the result of the much smaller Ru···Ru distance of only 7.25 Å across the croconate linker as compared to 9.28 Å in the dicarboxylate derivative. The small size of the macrocycle leads to a close approach of the sterically demanding P i Pr 3 ligands. Hence, the closest H···H distances of 2.48 Å are only marginally larger than the contact VAN-DER-WAALS distance of 2.40 Å. This not only suggests that the phosphine ligands act as umbrellas, but also that the croconate dianion is potentially one of the smallest bis(chelate)s capable of linking two Ru(CO)(P i Pr 3 ) 2 (CH=CH-R) fragments. The close approach of the Ru(P i Pr 3 ) 2 moieties also leads to an increase of the Ru-CH=CH angles to 138.1(4) or 140.4(4) • compared to the 129.7 (16) and 133.4 (14) • found in the 4-thioacetylisophthalate-linked congener [29], as well as a sizable torsion of 28.6 • of the Ru-CH=CH entities out of the plane of the phenylene linker to relieve steric strain between H α and the hydrogen atoms of the phenylene unit. This matches with the unusual low-field shift of the H α resonance in the 1 H-NMR spectrum (vide supra), indicating that the relatively large torsion is also retained in solution.
The five-membered ring formed by the croconate chelate and the ruthenium ion imposes an only modest distortion of the octahedral coordination geometry with O-Ru-O angles of 75.59 (13) • and 76.20(13) • , respectively. This is to be compared to 59.1(4) • and 58.5(4) • in the four-membered chelate rings formed by the carboxylates in the 4thioacetylisophthalate complex. Ruthenium coordination also localizes the C=C and C=O double bonds within the croconate ligand as is evident from the inequivalence of all C-C and C=O bonds. The non-coordinating carbonyl function is associated with the shortest C=O bond length of 1.229(6) Å, while the other CO bonds of 1.248(6) to 1.285(6) Å are appreciably longer. Likewise, the C3-C6 and C5-C6 bonds to the uncoordinated carbonyl group of the croconate ligand of 1.498(8) and 1.489(7) Å are longer than the remaining ones of 1.414(8), 1.446(7) and 1.457(7) Å, indicating that delocalization of the C=C double bond is confined to the two ketoenolate-type binding pockets (note that the C-C bonds in the dipotassium dihydrate salt are 1.465(3) and 1.474(3) Å) [72].

Electrochemistry
The electrochemical behavior of 2 Ru 2 Ph-Croc was probed in CH 2 Cl 2 as the solvent and was found to depend on the ion-pairing capabilities of the counter ion of the supporting electrolyte. With NBu 4 PF 6 , three anodic redox events can be observed at E 1/2 values of −134 mV, −17 mV and 207 mV. As is evident from Figure 6, the first two waves correspond to one-electron oxidations to the respective mono-and dication, whereas the third event entails two electrons and hence corresponds to the further charging to the tetracation. The use of NBu 4 BAr F 24 as the supporting electrolyte (BAr F 24 − = [B{C 6 H 3 (CF 3 -3,5) 2 } 4 ] − ) also resolves the third and fourth oxidations into separate one-electron processes and also increases the potential separations between the 0/+//+/2+ waves. The corresponding data are compiled in Table 1 along with those of the Ru 2 Ph-Cl precursor and the analogous iso-nicotinate-bridged tetraruthenium congener 2 Ru 2 Ph-m Py of Scheme 1. a Data for Ru2Ph-Cl and Ru2Phm Py a from ref. [27]. Similar responses, albeit with considerably lower redox splittings between the merged one-electron waves of the 0/2+ and 2+/4+ processes, were observed for other macrocyclic tetraruthenium complexes comprising of π-conjugated divinylarylene diruthenium building blocks. This pattern indicates the nearly coincident charging of the opposite {Ru}-CH=CH-Aryl-CH=CH-{Ru} electrophores by first one and then a second electron. The only small or vanishing potential splitting between the 0/+ and +/2+ or the 2+/3+ and 3+/4+ waves reflects electrostatic interactions between them, as the insulating dicarboxylate linkers prevent through-bond electronic coupling [27][28][29][30][31]. In this particular case,  Similar responses, albeit with considerably lower redox splittings between the merged one-electron waves of the 0/2+ and 2+/4+ processes, were observed for other macrocyclic tetraruthenium complexes comprising of π-conjugated divinylarylene diruthenium building blocks. This pattern indicates the nearly coincident charging of the opposite {Ru}-CH=CH-Aryl-CH=CH-{Ru} electrophores by first one and then a second electron. The only small or vanishing potential splitting between the 0/+ and +/2+ or the 2+/3+ and 3+/4+ waves reflects electrostatic interactions between them, as the insulating dicarboxylate linkers prevent through-bond electronic coupling [27][28][29][30][31]. In this particular case, however, an alternative scenario, where one of these composite waves originates from the first oxidations of opposite croconate linkers, is also conceivable, such that the loci of the individual oxidations remain to be verified. One should note here that neutral C 5 O 5 is only stable in water, forming leuconic acid, i.e., the pentaketone pentahydrate C 5 O 5 ·5H 2 O, whereas in other solvents the two-electron oxidized form is chemically unstable [54,68]. In spite of the presence of two kinds of electroactive building blocks, which are both capable of losing two electrons each, no further charging processes could be observed within the potential windows of the used electrolytes.
Irrespective of the identity of the oxidation sites, the use of a croconate instead of a dicarboxylate bis(chelate) increases the thermodynamic stabilities of the mixed-valent forms as evident from the enhanced ∆E 1/2 values between the 0/+ and +/2+, or the 2+/3+ and the 3+/4+ redox couples. While the sizable effects of the anion of the supporting electrolyte point towards a significant contribution of electrostatic effects to the observed redox splittings, electrochemical studies per se are inconclusive as to whether this increase in redox splitting also entails contributions from improved electron delocalization [73][74][75][76][77][78][79]. The presence or absence of electronic coupling can, however, be probed by combining (electro)chemical oxidation with IR and UV/Vis/NIR spectroscopy, and this is the subject of the following section.
In passing we add that cathodic scans reveal two closely spaced reductions associated with the croconate linkers as chemically only modestly reversible waves with half-wave potentials of −1930 mV and −2010 mV. In NBu 4 BAr F 24 the reduction can just be made out at the cathodic limit of the potential window as highly overlapped peaks with an associated average E 1/2 0/− of −2000 mV as determined through square-wave voltammetry. Graphical accounts of these waves can be found in the Supplementary Materials ( Figure S9). These processes are of no concern in the present context and are not further considered.

IR and UV/Vis/NIR Spectra of the Oxidized Forms and Insights from Quantum Chemistry
As was discussed in the previous section, molecular spectroscopy of the oxidized forms was conducted in order to provide answers to the open questions about the identity of the electron transfer sites in 2 Ru 2 Ph-Croc and on the existence and strength of any electronic coupling in the mixed-valent states. As to the first issue, the carbonyl ligands at the ruthenium ions and the characteristic carbonyl and CC stretches of the bridging ligands of 2 Ru 2 Ph-Croc offer convenient IR labels that respond to changing electron densities at these local sites as a result of the stepwise oxidations. While the spectroscopic responses expected of Ru 2 Ph-based redox processes are amply known, we also conducted similar studies on (NBu 4 + ) 2 croc 2− [80], which is soluble in CH 2 Cl 2 . Due to the inability of CH 2 Cl 2 to form strong hydrogen bonds, only the first oxidation is reversible (see Figure S10 of the Supplementary Materials) [54]. Accompanying spectroscopic changes in the IR spectrum are displayed in Figure S11 of the Supplementary Materials and entail the bleaching of the strong band at 1520 cm −1 and the emergence of a new, weaker band at 1552 cm −1 as the most characteristic changes.
The various oxidized forms 2 Ru 2 Ph-Croc n+ (n = 1-4) were electrogenerated inside a thin-layer electrolysis cell [81] by increasing the applied working potential in increments until a new equilibrium had established. Owing to the enhanced redox splittings and the higher propensity for also observing the mixed-valent states without interference from the bordering isovalent states, we conducted these experiments on solutions of the complex in 0.05 M NBu 4 BAr F 24 in the less volatile 1,2-dichloroethane (DCE) as the solvent. Graphical accounts of the outcomes of such experiments are displayed in Figure 7, while pertinent data are summarized in Table 2. The IR spectra of 2 Ru 2 Ph-Croc in the neutral as well as cationic states in the full range between 1000 cm −1 and 4000 cm −1 are shown in Figure S12 of the Supplementary Materials. Ru(CO) 1913, 1914 1918, 1920, 1934, 1942 1939, 1942, 1942, 1949 1939, 1939, 1945, 1948 1977, 1978, 1981, 1983 1983, 1984, 1988  In its neutral state, 2 Ru2Ph-Croc shows a single CO band for the ruthenium-bonded carbonyl ligands Ru(CO) at 1913 cm −1 (see top left graph of Figure 7). Despite the formally higher number of 18 valence electrons at each {Ru} fragment in 2 Ru2Ph-Croc, the absolute values for the Ru(CO) stretches match those of parent Ru2Ph-Cl, where the ruthenium ions attain a lower electron count of only 16 valence electrons. This contrasts sharply to a sizable red-shift by 13 cm −1 on introducing an acac − donor [82]. This difference is likely rooted in the combined effects of the limited electron donating capabilities of the croconate bridging ligand and attenuated π-conjugation along the divinylphenylene backbone due to the rather sizable torsion at the vinyl-phenylene linkages (vide supra). The position and overall shape of this band are fully reproduced by our quantum chemical calculations. The latter predict two nearly coincident peaks for the symmetric and antisymmetric stretches of the carbonyl ligands on two diagonally related {Ru} entities at 1913 and 1914 cm −1 . During the first oxidation an intricate Ru(CO) band pattern emerges, which could be deconvoluted into three distinct bands (see blue dashed lines in the top left graph of Figure 7). Such a three-band pattern is also predicted by the quantum chemical vibrational analysis with features at νcalc = 1920, 1934 and 1942 cm −1 , in excellent agreement with the experiment. Based on the calculations and chemical intuition, the band found at 1917 cm −1 can be assigned to the carbonyl stretch of the remaining neutral Ru2Ph unit. The remaining two bands at 1935 and 1949 cm −1 correspond to the less intense symmetric and the intense antisymmetric combinations of Ru(CO) stretches of the one-electron oxidized divi- In its neutral state, 2 Ru 2 Ph-Croc shows a single CO band for the ruthenium-bonded carbonyl ligands Ru(CO) at 1913 cm −1 (see top left graph of Figure 7). Despite the formally higher number of 18 valence electrons at each {Ru} fragment in 2 Ru 2 Ph-Croc, the absolute values for the Ru(CO) stretches match those of parent Ru 2 Ph-Cl, where the ruthenium ions attain a lower electron count of only 16 valence electrons. This contrasts sharply to a sizable red-shift by 13 cm −1 on introducing an acac − donor [82]. This difference is likely rooted in the combined effects of the limited electron donating capabilities of the croco-nate bridging ligand and attenuated π-conjugation along the divinylphenylene backbone due to the rather sizable torsion at the vinyl-phenylene linkages (vide supra). The position and overall shape of this band are fully reproduced by our quantum chemical calculations. The latter predict two nearly coincident peaks for the symmetric and antisymmetric stretches of the carbonyl ligands on two diagonally related {Ru} entities at 1913 and 1914 cm −1 . During the first oxidation an intricate Ru(CO) band pattern emerges, which could be deconvoluted into three distinct bands (see blue dashed lines in the top left graph of Figure 7). Such a three-band pattern is also predicted by the quantum chemical vibrational analysis with features at ∼ ν calc = 1920, 1934 and 1942 cm −1 , in excellent agreement with the experiment. Based on the calculations and chemical intuition, the band found at 1917 cm −1 can be assigned to the carbonyl stretch of the remaining neutral Ru 2 Ph unit. The remaining two bands at 1935 and 1949 cm −1 correspond to the less intense symmetric and the intense antisymmetric combinations of Ru(CO) stretches of the one-electron oxidized divinylphenylene diruthenium moiety. The overall Ru(CO) band shifts and the increased energy difference between the two vibrational modes of the oxidized divinylphenylene diruthenium entity are in complete agreement with the oxidation-induced changes for other, related complexes of this type [49][50][51][52]83,84]. During the second oxidation, the band at 1917 cm −1 starts to fade, while the other two bands shift further blue, to 1941 and 1952 cm −1 . The pattern of one intense and one less intense Ru(CO) band is a clear indicator that the di-cation 2 Ru 2 Ph-Croc 2+ is comprised of two singly oxidized Ru 2 Ph •+ entities. A further argument against croconate-based oxidations is provided by the very modest red/blue shifts of the croconate CO bands corresponding to the chelating (1503 cm −1 → 1499 cm −1 → 1498 cm −1 ) and the uncoordinated keto CO functions (1630 cm −1 → 1636 cm −1 → 1639 cm −1 ). The experimental band pattern is well matched by the computed Ru(CO) vibrational modes at ∼ ν calc = 1939, 1939, 1945 and 1948 cm −1 based on an input structure in the electronic triplet state. We note here that the data for the singlet form are very similar (see Table 2). The latter is by a computed margin of 60 kJ/mol higher in energy and its calculated spectros-copic features agree less well with our experimental data (vide infra). The mixed-valent form 2 Ru 2 Ph-Croc •+ is thus characterized by a pattern of bands corresponding to the neutral and a double Ru(CO)-band-feature for the oxidized Ru 2 Ph subunits. The blue shift of the Ru 2 Ph subunit of 2 Ru 2 Ph-Croc + by 3 cm −1 and the (averaged) red-shift of the Ru 2 Ph •+ entity of 4 cm −1 with respect to the bordering isovalent states 2 Ru 2 Ph-Croc and 2 Ru 2 Ph-Croc 2+ are very modest and may well be entirely due to inductive effects transmitted via the croconate linkers [82,85].
In line with the above presumption, further oxidation did not provide any indication for a unique spectroscopic signature of a tricationic form, either in the IR nor the NIR region of the spectrum. This implies that the triacation has no specific spectroscopic fingerprint distinguishing it from mixtures of the di-and the tetracation. Hence, only the disappearance of the bands associated to the monocationic Ru 2 Ph •+ fragments with concomitant formation of a new Ru(CO) band at 1982 cm −1 were observed. The presence of only a single, shifted Ru(CO) band is a clear indicator that the second pair of oxidations also involves the Ru 2 Ph entities and that all four {Ru} nodes are electronically equivalent. Again, the shift of vibrational bands associated with the croconate ligands are modest (1639 cm −1 → 1644 cm −1 ; 1498 cm −1 → 1493 cm −1 ), which argues against any substantial involvement into also the higher oxidations. The computed energies of the carbonyl stretches agree well with the experimental data, regardless of the assumed spin state. Based on the results for dications of other divinylphenylene diruthenium complexes or their respective forms when arranged in a macrocycle, the singlet state should dominate [27,86]. The experimental finding of hardly any involvement of the croconate ligands to the individual redox processes is fully supported by our natural bond order (NBO) analysis ( Figure 8). According to our calculations, the first two oxidations result in a charge loss of only 7% from the croconate ligands, with an additional 6% loss during the last two-electron charging process.
Based on the experimental results and in full agreement with our computations, it can be said that the mixed-valent mono-and trioxidized forms are best described as containing one neutral Ru 2 Ph/singly oxidized Ru 2 Ph •+ and one singly oxidized Ru 2 Ph •+ /doubly oxidized Ru 2 Ph 2+ entity. The accessibility and unique spectroscopic features of the moncation are thus likely the result of electrostatic repulsion caused by the close spatial proximity of the two redox-active divinylphenylene diruthenium fragments and to only a minor degree (if any at all) to electronic coupling across the croconate ligands.
This issue of electronic coupling in the mixed-valent states is, however, best probed by electronic spectroscopy in the near infrared (NIR). All redox states that were accessible in the IR spectroelectrochemical experiments could also be interrogated by UV/Vis/NIR spectroscopy. Corresponding data derived from our experiments and from time-dependent density functional theory (TD-DFT) calculations are collected in Table 3. Our TD-DFT protocols match those of other authors that have proven successful for assessing the optical properties of organic dyes and metal complexes [87][88][89]. The electronic spectrum of neutral 2 Ru 2 Ph-Croc and its change during the first oxidation to the radical cation are depicted in Figure 9.  Based on the experimental results and in full agreement with our comp can be said that the mixed-valent mono-and trioxidized forms are best descri taining one neutral Ru2Ph / singly oxidized Ru2Ph  + and one singly oxidized R doubly oxidized Ru2Ph 2+ entity. The accessibility and unique spectroscopic fea moncation are thus likely the result of electrostatic repulsion caused by the c proximity of the two redox-active divinylphenylene diruthenium fragments a minor degree (if any at all) to electronic coupling across the croconate ligand This issue of electronic coupling in the mixed-valent states is, however, by electronic spectroscopy in the near infrared (NIR). All redox states that wer  in the IR spectroelectrochemical experiments could also be interrogated by UV/Vis/NIR spectroscopy. Corresponding data derived from our experiments and from time-dependent density functional theory (TD-DFT) calculations are collected in Table 3. Our TD-DFT protocols match those of other authors that have proven successful for assessing the optical properties of organic dyes and metal complexes [87][88][89]. The electronic spectrum of neutral 2 Ru2Ph-Croc and its change during the first oxidation to the radical cation are depicted in Figure 9.
In contrast to other dicarboxylate-based macrocycles, 2 Ru2Ph-Croc is not faint yellow in color, but brown. This is due to the presence of a broad absorption shoulder near 550 nm. The underlying excitations, computed at λcalc = 608 to 626 nm, entail charge transfer (CT) from the Ru2Ph moieties to the oxocarbon bridging ligands (see Figures S13-S16 in the Supplementary Materials for plots of the MOs involved in the transitions and electron density difference maps). Revealingly, the two highest occupied MOs as well as the two In contrast to other dicarboxylate-based macrocycles, 2 Ru 2 Ph-Croc is not faint yellow in color, but brown. This is due to the presence of a broad absorption shoulder near 550 nm. The underlying excitations, computed at λ calc = 608 to 626 nm, entail charge transfer (CT) from the Ru 2 Ph moieties to the oxocarbon bridging ligands (see Figures S13-S16 in the Supplementary Materials for plots of the MOs involved in the transitions and electron density difference maps). Revealingly, the two highest occupied MOs as well as the two lowest unoccupied MOs are doubly degenerate and represent the in-and out-of-phase combinations of orbitals localized at the oppositely disposed Ru 2 Ph entities (HOMO, HOMO−1) or on the coroconate ligands (LUMO, LUMO+1). This degeneracy is a token for the lack of electronic interactions between the individual donor or acceptor fragments within the macrocyclic structure [83,90]; for an example of a π-conjugated organic macrocycle with non-degenerate FMOs and electronically coupled mixed-valent states see Ref. [91]. A shoulder at λ max = 410 nm corresponds to the HOMO−2 to LUMO excitation and is hence characterized as a croconate-based π-π* transition with a calculated wavelength λ calc of 431 nm. The most intense band at λ max = 350 nm (λ calc = 329 nm) corresponds to the π-π* transition on the divinylphenylene diruthenium (Ru 2 Ph) fragments.
The most characteristic feature of the spectrum of 2 Ru 2 Ph-Croc •+ are the three bands in the near infrared (NIR) at 1275 nm (7830 cm −1 ), 1085 nm (9300 cm −1 ), and 930 nm (10770 cm −1 ) in the order of decreasing absorbance (see blue broken lines in Figure 9, left). Furthermore, the Vis band at ca. 550 nm intensifies without any discernible shift, while the UV band originating from the Ru 2 Ph chromophores decreases in intensity. The rich structuration of the NIR absorption is characteristic for the radical cation of divinylphenylenebridged diruthenium complexes and is traced back to vibrational coupling [47,86]. The strong similarities in the NIR absorbance between the radical cation 2 Ru 2 Ph-Croc •+ and those of other tetranuclear metallamacrocycles with dicarboxylate linkers and the absence of any further absorption at lower energies, as would be expected in the case of intervalence charge transfer (IVCT) between the differently charged Ru 2 Ph subunits, equally suggest that the croconate bridges remain uninvolved in the oxidation and act as insulators.
The TD-DFT calculations paint a somewhat different picture. They assign the prominent feature at λ calc = 1011 nm to a transition, which involves charge transfer from a combination of orbitals that spread over both croconate bridging ligands and the remaining reduced divinylphenylene diruthenium building block (mainly the β-HOSO-1, see the Supplementary Materials). Judging by the TD-DFT results, this band is hence assigned as a mixed oxocarbon to Ru 2 Ph •+ charge transfer (L-L'MCT) and IVCT rather than being confined to the oxidized divinylphenylene diruthenium unit. This seems, however, unlikely for the mentioned reasons and the alterations in the IR/NIR spectra imposed by further ox-idation to the dication (vide infra). It thus appears as if the quantum chemical calculations overestimate the strength of adiabatic electronic coupling, which is a common problem of DFT-based methods [92,93]. TD-DFT also predicts an additional band at λ max = 2171 nm, albeit with a very small oscillator strength, which has all characteristics of a classical IVCT transition. It is thus directed from the reduced to the singly oxidized divinylphenylene diruthenium entity. No such feature was, however, experimentally observed. The overestimated computed electron delocalization only affects the transitions in the NIR. All other bands in the Vis or the UV are adequately represented. Thus, the split band at λ max = 570 and 535 nm corresponds to predicted transitions at λ calc = 663 and 505 nm. They both entail CT from one of the Ru 2 Ph entities to the croconate linkers, with the excitation involving the oxidized Ru 2 Ph •+ subunits at the higher energy. The π-π* transitions confined to the oxocarbon bridges as well as that on the reduced Ru 2 Ph entity remain basically unaltered during the first oxidation.
Further oxidation to the dication is characterized by the continued intensity gain of all bands in the visible and near infrared (NIR) regions of the electromagnetic spectrum ( Figure  10, orange spectral lines). A closer look at the NIR spectrum (right panel in Figure 10) provides a more detailed account of the subtle changes in the positions and intensities of the individual peaks as obtained from spectral deconvolution (broken blue and orange spectral lines). This close-up view also reveals that no additional IVCT band was present in 2 Ru 2 Ph-Croc •+ which would bleach during the second oxidation. While the smallest feature, formally at 10,770 cm −1 (930 nm), remains basically unaltered during the second oxidation ( ∼ ν max = 10,775 cm −1 ), the second peak, formerly located at 9300 cm −1 (1085 nm), is slightly shifted blue to 9370 cm −1 (1067 nm) whilst also becoming the most prominent NIR feature. The NIR peak at the lowest energy is shifted blue from 7830 cm −1 (1275 nm) to 8065 cm −1 (1240 nm). Such blue shift of the NIR absorption bands during successive charging processes of macrocycles built from divinylphenylene diruthenium (Ru 2 Ph) complex fragments was also observed for the carboxylate-bridged variants, in particular when the individual Ru 2 Ph subunits are bridged by relatively short linkers. This holds true even in cases, where the intermediate one-electron oxidized forms could not be observed as se-parate species due to a too close proximity of the individual one-electron waves. These shifts were hence ascribed to decreased electron-donating capabilities of the bis(chelating) bridging ligand as peripheral redox sites are oxidized stepwise, i.e., to inductive effects [86]. The fact that this influence is particularly large in the smaller macrocycle 2 Ru 2 Ph-Croc with an even closer transannular distance between the two Ru 2 Ph fragments supports this notion further. 8065 cm −1 (1240 nm). Such blue shift of the NIR absorption bands during successive charging processes of macrocycles built from divinylphenylene diruthenium (Ru2Ph) complex fragments was also observed for the carboxylate-bridged variants, in particular when the individual Ru2Ph subunits are bridged by relatively short linkers. This holds true even in cases, where the intermediate one-electron oxidized forms could not be observed as separate species due to a too close proximity of the individual one-electron waves. These shifts were hence ascribed to decreased electron-donating capabilities of the bis(chelating) bridging ligand as peripheral redox sites are oxidized stepwise, i.e., to inductive effects [86]. The fact that this influence is particularly large in the smaller macrocycle 2 Ru2Ph-Croc with an even closer transannular distance between the two Ru2Ph fragments supports this notion further. Dioxidized 2 Ru2Ph-Croc 2+ has again an inherently symmetric electron density distribution as both Ru2Ph units are present in their singly oxidized states. This precludes IVCT transitions between identical subunits and leads to again a good match between experimental and calculated spectra if a triplet state is assumed. As it is evident from the computed spectrum shown as Figure S15 in the Supplementary Materials, the NIR absorption Dioxidized 2 Ru 2 Ph-Croc 2+ has again an inherently symmetric electron density distribution as both Ru 2 Ph units are present in their singly oxidized states. This precludes IVCT transitions between identical subunits and leads to again a good match between experimental and calculated spectra if a triplet state is assumed. As it is evident from the computed spectrum shown as Figure S15 in the Supplementary Materials, the NIR absorption is calculated to comprise of two transitions at λ calc = 923 and 928 nm of highly mixed cha-racter, which involve the highest two occupied and lowest two unoccupied spin orbitals β-HOSO−1 to β-LUSO+1. Both members of each pair are again nearly degenerate sets of in-and out-of-phase combinations of MOs confined to the Ru 2 Ph entities, as was the case for the neutral form. However, in reality, individual excitations are confined to only one open-shell Ru 2 Ph •+ entity. Our TD-DFT calculations correctly predict the presence of the charge-transfer transition from the two Ru 2 Ph •+ fragments to the croconate linkers. The invariance of the absorption feature at λ max = 410, corresponding to the croconate-based π-π* transition, both in terms of intensity and energy, further indicates a redox-innocent behavior of this ligand within this macrocyclic system.
As was found in the IR experiment, UV/Vis/NIR spectroelectrochemistry fails to resolve the third and fourth oxidations of 2 Ru 2 Ph-Croc into individual one-electron steps. This means that the intermediate trication has no specific absorption profile that would set it apart from mixtures of the two-and fourfold oxidized forms. The evolution of the spectra during these final two oxidations are displayed in Figure 11. The final two-electron oxidation entails the continuous bleach of all NIR bands, signaling the absence of mixedvalent Ru 2 Ph •+ moieties within the macrocycle (note that the small residual intensity in the NIR signals that complete conversion to the tetracation could not be achieved whilst maintaining reasonable voltage levels so as to avoid decomposition). We also wish to point out that at no stage of the final oxidation we observed the initial growth and decrease of a new NIR band that would indicate IVCT between the monooxidized Ru 2 Ph •+ and the dioxidized Ru 2 Ph 2+ entities (note that, even in the absence of a redox splitting, the trication will be the dominant species at some point during the electrolysis) [27]. Comparison between experimental and calculated UV/Vis/NIR spectra suggests that 2 Ru 2 Ph-Croc 4+ seems to behave analogous to the precursor complex and other macrocycles containing dioxidized Ru 2 Ph 2+ fragments. Thus, the singlet state constitutes the dominant form at r.t. that at no stage of the final oxidation we observed the initial growth and decrease of a new NIR band that would indicate IVCT between the monooxidized Ru2Ph •+ and the dioxidized Ru2Ph 2+ entities (note that, even in the absence of a redox splitting, the trication will be the dominant species at some point during the electrolysis) [27]. Comparison between experimental and calculated UV/Vis/NIR spectra suggests that 2 Ru2Ph-Croc 4+ seems to behave analogous to the precursor complex and other macrocycles containing dioxidized Ru2Ph 2+ fragments. Thus, the singlet state constitutes the dominant form at r.t. The UV/Vis spectrum of 2 Ru2Ph-Croc 4+ is dominated by a strong absorption band at λmax = 605 nm, which is very characteristic to the bipolaron state of dioxidized divinylphenylene diruthenium complexes. The increased ring strain and the ensuing unusually large torsion at the vinyl phenylene linkages in the present macrocycle seem to have a detrimental effect on the absorptivity, as the molar extinction coefficient is smaller by ca. 25% as compared to those obtained for related isophthalate-bridged systems [27,29,32,86]. This band is replicated well by the quantum chemical calculations (λcalc = 574 nm). Corresponding contour and EDDM plots are shown as Figure S16 in the Supplementary Materials. The second relevant absorption feature is the π-π* transition on the croconate bridging ligands. While the energy of this band (λmax = 410 nm) is still unaltered, it The UV/Vis spectrum of 2 Ru 2 Ph-Croc 4+ is dominated by a strong absorption band at λ max = 605 nm, which is very characteristic to the bipolaron state of dioxidized divinylphenylene diruthenium complexes. The increased ring strain and the ensuing unusually large torsion at the vinyl phenylene linkages in the present macrocycle seem to have a detrimental effect on the absorptivity, as the molar extinction coefficient is smaller by ca. 25% as compared to those obtained for related isophthalate-bridged systems [27,29,32,86]. This band is replicated well by the quantum chemical calculations (λ calc = 574 nm). Corresponding contour and EDDM plots are shown as Figure S16 in the Supplementary Materials. The second relevant absorption feature is the π-π* transition on the croconate bridging ligands. While the energy of this band (λ max = 410 nm) is still unaltered, it gains further in intensity. Our quantum chemical calculations put it at λ max = 400 nm with main contributions from the HOMO and LUMO+2, the latter corresponding to the HOMO−2 and LUMO of the neutral state. The overall scenario that emerges from our investigations is therefore the same as that in the dicarboxylate-bridged macrocyclic structures in that both Ru 2 Ph n+ chromophores act independently of each other and "just happen to be chemically linked".

EPR Spectroscopy
All accessible oxidized forms of 2 Ru 2 Ph-Croc were also investigated by means of EPR spectroscopy. Samples of the respective oxidized forms for EPR detection were generated by chemically oxidizing 2 Ru 2 Ph-Croc with appropriate amounts of ferrocenium hexa-fluorophosphate (FcH + PF 6 − , E 1/2 = 0 mV; 0.8 equiv. for selective formation of 2 Ru 2 Ph-Croc + , 2.5 equiv. for quantitative formation of 2 Ru 2 Ph-Croc 2+ ), or 4.4 equiv. of 1,1 -diacetylferrocenium hexafluoroantimonate (Ac 2 Fc + SbF 6 − , E 1/2 = 490 mV for 2 Ru 2 Ph-Croc 4+ ). Their EPR-inactivity above the temperature of liquid helium renders these ferrocenium salts ideally suited for this purpose. The identity of the respective oxidized species was verified by comparing the characteristic Ru(CO) stretches of the chemically oxidized complexes to the spectra obtained via IR-SEC.
The radical cation 2 Ru 2 Ph-Croc •+ is characterized by an isotropic signal in fluid solution, over a temperature range of +20 • C down to −50 • C, and in the frozen glass at −140 • C (see top left and middle panels of Figure 12). The g iso -value of 2.012 is notably close to the free electron value g e = 2.0023, but larger than that of 1.998 for the tetra n butylammonium salt of the croconate radical anion (see Figure S17 of the Supporting Materials). The position is typical of the radical cations of divinylarylene-bridged diruthenium complexes and indicates that the spin-bearing orbital is dominated by the π-conjugated divinylphenylene linker, despite the relatively large torsion around the Ru-CH=CH-C 6 H 4 linkages. Quantum chemical calculations indeed place the entire unpaired spin density at the oxidized Ru 2 Ph •+ site (note, however that the calculations falsely predict equal spin densities on both Ru 2 Ph entities as shown in Figure S18 in the Supplementary Materials). Oxidation of the second Ru 2 Ph entity to furnish the dication 2 Ru 2 Ph-Croc 2+ results in a EPR spectrum, which is virtually identical to that of the monocation, in fluid solution and in the frozen glass (see the middle row of Figure 12). This underlines the localized nature of the two mutually independent spins in the dication 2 Ru 2 Ph-Croc 2+ . DFT again confirms this view as it assigns no probability density of the unpaired electrons to the croconate bridges.
As it is typical for compounds containing the dioxidized Ru 2 Ph 2+ complex fragment, the tetracation 2 Ru 2 Ph-Croc 4+ is also EPR-active at room temperature, although the electronic spectra were better matched by assuming a singlet ground state. The open-shell form is hence thermally accessible. The relatively high charge on this small cyclic molecule renders 2 Ru 2 Ph-Croc 4+ too unstable to allow for EPR measurements at +20 • C without competing decomposition. The spectrum in fluid solution was hence recorded at −20 • C. Under these conditions, a slightly anisotropically broadened signal is observed with a g ave -value of 2.026 (see Figure 12, bottom left), which is again typical of such species [27,29,32,86]. The slightly increased g value points to higher spin densities at the ruthenium ions. This also becomes evident from the axial EPR signal detected at −140 • C with associated g-values of g z = 2.040 and g x , g y = 2.015. Mirroring the charge distributions, only 1% of the unpaired spin density are computed to reside on the croconate ligands in the open-shell quintet state.
ties on both Ru2Ph entities as shown in Figure S18 in the Supplementary Materials). Oxi-dation of the second Ru2Ph entity to furnish the dication 2 Ru2Ph-Croc 2+ results in a EPR spectrum, which is virtually identical to that of the monocation, in fluid solution and in the frozen glass (see the middle row of Figure 12). This underlines the localized nature of the two mutually independent spins in the dication 2 Ru2Ph-Croc 2+ . DFT again confirms this view as it assigns no probability density of the unpaired electrons to the croconate bridges. As it is typical for compounds containing the dioxidized Ru2Ph 2+ complex fragment, the tetracation 2 Ru2Ph-Croc 4+ is also EPR-active at room temperature, although the electronic spectra were better matched by assuming a singlet ground state. The open-shell form is hence thermally accessible. The relatively high charge on this small cyclic molecule renders 2 Ru2Ph-Croc 4+ too unstable to allow for EPR measurements at +20 °C without competing decomposition. The spectrum in fluid solution was hence recorded at −20 °C. Under these conditions, a slightly anisotropically broadened signal is observed with a gavevalue of 2.026 (see Figure 12, bottom left), which is again typical of such species

Conclusions
Three lessons can be learned from our study of the tetraruthenium metallamacrocyclic complex 2 Ru 2 Ph-Croc: (i) Incorporation of an intrinsically redox-active ligand into the coordination sphere of a likewise redox-active metal-coligand entity does not necessarily guarantee that this ligand retains this property in the resulting complex. Despite very similar redox potentials of the croconate (C 5 O 5 2− ) ligands and the employed {Cl(P i Pr 3 ) 2 (CO)Ru} 2 (µ-CH=CH-C 6 H 4 -CH=CH) (Ru 2 Ph-Cl) building blocks, all accessible oxidations of 2 Ru 2 Ph-Croc are confined to the Ru 2 Ph entities. (ii) In spite of the sizable redox splittings between the first and, in the very weakly ion pairing NBu 4 [B{C 6 H 3 (3,5-CF 3 )} 4 ] − (NBu 4 BAr F 24 ) electrolyte, also the second charging processes of the two {Ru} 2 (µ-CH=CH-C 6 H 4 -CH=CH) entities, and in spite of the specific IR, UV/Vis profiles of the mo-nocation 2 Ru 2 Ph-Croc •+ , even with detectable Ru(CO) band shifts of all Ru(CO) nodes, the mixed-valent forms exhibit different charge states on the opposite Ru 2 Ph n+ entities and are strictly valence-localized species. 2 Ru 2 Ph-Croc therefore constitutes a particularly instructive and potentially misleading example of a so-called "pretender" [78], which owes the ensuing thermodynamic stabilities of its mixed-valent forms exclusively to electrostatic interactions. In the case of 2 Ru 2 Ph-Croc, this is probably due to the very small dimensions of the macrocycle and the close spatial proximity of the Ru 2 Ph electrophores. (iii) The clear identification of a specific intervalence charge transfer (IVCT) band is about the only truly reliable indicator of electronic coupling in mixed-valent systems and must be critically evaluated. This is particularly important as common computational approaches based on (TD)-DFT are still prone to overestimating π-delocalization effects.

Computational Details
The ground state electronic structures of the (model) complexes were calculated by density functional theory (DFT) methods using the Gaussian 16 program packages [94]. Geometry optimizations were performed without any symmetry constraints. Open-shell systems were calculated by the unrestricted KOHN-SHAM approach (UKS). Geometry optimization and subsequent vibrational analysis was performed in solvent media. Solvent effects were described by the polarizable continuum model (PCM) with standard parameters for dichloromethane or 1,2-dichloroethane [95,96]. Explementary input files are included as Tables S4-S6 in the Supplementary Materials. The output values of the vibrational analysis were corrected for their offset in zero-point energies dependent on the utilized combination of functional and basis set functions by multiplication with a vibrational frequency scaling factor. This factor is 0.950 for the combination of PBE0/6-31G(d) (see the NIST Standard Reference Database. Precomputed vibrational scaling factors. https://cccbdb.nist.gov/vibscalejust.asp (accessed on 27 August 2021)). Electronic spectra were calculated by the TD-DFT method on optimized geometries. The quasirelativistic WOOD-BORING small-core pseudopotentials (MWB) [97,98] and the corresponding optimized set of basis functions for Ru atoms [99] as well as 6-31G(d) polarized double-ζ basis sets for the remaining atoms [100] were employed together with the PERDEW, BURKE, ERNZERHOF exchange and correlation functional (PBE0 aka. PBE1PBE) [101,102] The Gauss-Sum program package was used to analyze the results [102], while the visualization of the results was performed with the Avogadro program package [103]. Graphical representations of molecular orbitals were generated with GNU Parallel [104] and plotted using the VMD program package [105] in combination with POV-Ray [106].

Materials and Methods
1 H-, 13 C{ 1 H}-and 31 P{ 1 H} spectra were recorded using Bruker Avance III 400 MHz (B H = 400 MHz, B P = 162 MHz) and Bruker Avance NEO 800 (B H = 800 MHz, B C = 202 MHz) spectrometers obtained from Bruker BioSpin MRI GmbH, Karslruhe, Germany. Spectral shifts are given in ppm and were referenced to the residual protonated solvent ( 1 H), the solvent signal ( 13 C), or to external for 87% H 3 PO 4 .
All electrochemical experiments were performed with a computer-controlled BASi potentiostat from Bioanalytical Systems, West Lafayette, IN, USA, in a custom-built, cylindrical, vacuum-tight one-compartment cell. A spiral-shaped Pt wire and an Ag wire, covered with electrodeposited AgCl, as the counter and pseudo-reference electrodes are sealed into glass capillaries that are introduced via Quickfit screws at opposite sides of the cell. A platinum working electrode (diameter 1.6 mm, from BASi is introduced through the top port via a PTFE screw cap with a suitable fitting. It is polished with first 1.0 mm and then 0.25 mm diamond pastes (Buehler-Wirtz, Lake Bluff, IL, USA) before measurements. To exclude the presence of O 2 and N 2 , the cell and solvent (ca. 7 mL of CH 2 Cl 2 ) are purged by bubbling a continuous stream of argon through the solution for several minutes. NBu 4 PF 6 (0.1 M) or NBu 4 BAr F 24 (0.05 M) were used as the supporting electrolyte. Internal referencing was done by the addition of equimolar amounts of decamethylferrocene (Cp* 2 Fe) after all data of interest had been acquired. Final referencing was done against the ferrocene/ferrocenium (Cp 2 Fe 0/+ ) redox couple with E 1/2 (Cp* 2 Fe 0/+ ) = −550 mV with NBu 4 PF 6 or E 1/2 (Cp* 2 Fe 0/+ ) = −620 mV with NBu 4 BAr F 24 as the supporting electrolyte. IR and UV/Vis/NIR spectroelectrochemistry was conducted inside a custom-built optically transparent thin-layer electrochemical (OTTLE) according the design of Hartl and coworkers [81]. It comprises of a Pt-mesh working and counter electrode and a thin silver plate as the pseudo-reference electrode sandwiched between the CaF 2 windows of a conventional liquid IR cell. For the SEC measurements, increased supporting electrolyte concentrations of 0.25 M for NBu 4 PF 6 or 0.1 M for NBu 4 BAr F 24 and 1,2-dichloroethane as the solvent were used. For the spectroelectrochemical experiments a Wenking POS2 potentiostat (Bank Elektronik-Intelligent Controls GmbH, Pohlheim, Germany) was used.
UV/Vis/NIR spectra were obtained on a TIDAS fiberoptic diode array spectrometer (combined MCS UV/NIR and PGS NIR instrumentation) from J&M Analytik AG, Essingen, Germany in a range between 250 nm to 2100 nm in HELLMA quartz cuvettes with 0.1 cm optical path length. Electron paramagnetic resonance (EPR) studies were performed on a tabletop X-band spectrometer MiniScope 400 with the matching temperature controller model H03, both manufactured by Magnettec GmbH, Berlin, Germany. Samples for EPR spectroscopy were prepared from the diamagnetic forms by chemical oxidation with either ferrocenium hexafluorophosphate (FcHPF 6 , E 1/2 = 0 mV; 0.8 equiv. for selective formation of 2 Ru 2 Ph-Croc + , 2.5 equiv. for quantitative formation of 2 Ru 2 Ph-Croc 2+ ), or 4.4 equiv. of 1,1 -diacetylferrocenium hexafluoroantimonate (Ac 2 FcSbF 6 , E 1/2 = 490 mV for 2 Ru 2 Ph-Croc 4+ ) as the oxidants. The EPR spectrum of 2 Ru 2 Ph-Croc 4+ was simulated with MATLAB using the EasySpin tool box (v. 5.2.8, see: http://easyspin.org/ (accessed on 27 August 2021)) [107], using the core-function 'garlic' for isotropic signals obtained from measurements in fluid solution and 'pepper' for anisotropic signals obtained from measurements in the frozen glass. Mass spectrometric measurements were conducted by Dr. Nicole Orth within the facilities of Prof. Dr. Ivanović-Burmazović at the Friedrich-Alexander-University of Erlangen-Nürnberg on an UHR-ToF Bruker Daltonik maXis plus instrument (Bruker Daltonik GmbH, Bremen, Germany), an ESI-quadrupole time-of-flight (qToF) mass spectrometer capable of resolution of at least 60.000 FWHM, coupled to a Bruker Daltonik cryospray unit. Detection was done in the positive-ion mode with the source voltage set to 4.5 kV. The dry gas (N2) was held at −85 • C and the spray gas at −90 • C. The spectrometer was calibrated with ESI-ToF Low Concentration Tuning Mix from Agilent prior to every measurement. All measurements were carried out using dichloromethane as the solvent. All measurements were carried out using dichloromethane as the solvent. FT-IR spectra were recorded using a Bruker Tensor II FT-IR spectrometer (Bruker, Billerica, MA, USA).
X-Ray diffraction analysis was performed on a STOE IPDS-II diffractometer (STOE&CIE GmbH, Darmstadt, Germany) equipped with a graphite monochromated MoK α radiation source (λ = 0.71073 Å) and an image plate detection system at T = 100.15 K. Using Olex2 [71], the structures were solved with the ShelXT [108] structure solution program using Intrinsic Phasing and refined with the ShelXL [108] refinement package using Least Squares minimization. Hydrogen atoms were introduced at their calculated positions. Structure plots were generated with the Platon program [70].

Synthesis and Isolation of Pure 2 Ru 2 Ph-Croc
The procedure detailed below has been optimized to enable the isolation of the pure tetranuclear macrocycle. Deviation from this combination of solvents as well as concentrations, reaction temperature and time is discouraged and will very probably lead to impure material. 180 mg (0.16 mmol, 1 eq) of Ru 2 Ph-Cl were dissolved in 6 mL of benzene and 39 mg (0.17 mmol, 1.06 eq) of potassium croconate monohydrate were dissolved in 3 mL of H 2 O. The combined emulsion was stirred for 19 hours at 35 • C. The precipitated solid was separated from the emulsion by centrifugation. The greenish brown solid was dried under vacuo. 49 mg (0.021mmol) of brown 2 Ru 2 Ph-Croc were obtained, corresponding to a yield of 21%. 1  The supernatant obtained after centrifugation of the reaction mixture from Section 4.3.1 was diluted with dichloromethane and water. The layers were separated and the organic layer washed twice with 5 mL of water to remove excess croconate ligand. The organic layer was subsequently dried over Na 2 SO 4 (the use of MgSO 4 is discouraged due to the instability of vinyl ruthenium species towards Lewis acids). After solvent removal under reduced pressure the brown residue was washed with small portions of first MeOH and the hexane (<1 mL) utilizing ultra-sonication and centrifugation to remove rutheniumcontaining decomposition products. The obtained reddish brown solid contains both, 2 Ru 2 Ph-Croc and 4 Ru 2 Ph-Croc, which were found to be impossible to separate and purify to a satisfying degree.

Conflicts of Interest:
The authors declare no conflict of interest.
Sample Availability: Not available.

Abbreviations
The following abbreviations are used in this manuscript: [9]