Alternative Approach for Specific Tyrosinase Inhibitor Screening: Uncompetitive Inhibition of Tyrosinase by Moringa oleifera

Tyrosinase (TYR) is a type III copper oxidase present in fungi, plants and animals. The inhibitor of human TYR plays a vital role in pharmaceutical and cosmetic fields by preventing synthesis of melanin in the skin. To search for an effective TYR inhibitor from various plant extracts, a kinetic study of TYR inhibition was performed with mushroom TYR. Among Panax ginseng, Alpinia galanga, Vitis vinifera and Moringa oleifera, the extracts of V. vinifera seed, A. galanga rhizome and M. oleifera leaf reversibly inhibited TYR diphenolase activity with IC50 values of 94.8 ± 0.2 µg/mL, 105.4 ± 0.2 µg/mL and 121.3 ± 0.4 µg/mL, respectively. Under the same conditions, the IC50 values of the representative TYR inhibitors of ascorbic acid and kojic acid were found at 235.7 ± 1.0 and 192.3 ± 0.4 µg/mL, respectively. An inhibition kinetics study demonstrated mixed-type inhibition of TYR diphenolase by A. galanga and V. vinifera, whereas a rare uncompetitive inhibition pattern was found from M. oleifera with an inhibition constant of Kii 73 µg/mL. Phytochemical investigation by HPLC-MS proposed luteolin as a specific TYR diphenolase ES complex inhibitor, which was confirmed by the inhibition kinetics of luteolin. The results clearly showed that studying TYR inhibition kinetics with plant extract mixtures can be utilized for the screening of specific TYR inhibitors.


Introduction
Treatment of skin disorders such as melasma, freckles, dark spots and hyperpigmentation is of great importance in the development of pharmaceutical and cosmetic products. The main cause of these problems is due to the accumulation of large amounts of melanin, produced by melanogenesis of melanosomes in the keratinocytes of the epidermis. Although melanogenesis occurs as a protective mechanism of the skin from UV exposure, unusual melanin stimulation produces free radicals causing wrinkles and damage to skin cell integrity [1]. Tyrosinase (TYR) is known as a key enzyme in the controlling step of the melanogenesis pathway [2]. The redox active type III copper-containing enzyme, belonging to the polyphenol oxidases, catalyzes two consecutive reactions of monooxygenase and oxidase [3]. The first reaction is known as monophenolase because L-tyrosine is oxygenated to L-dihydroxyphenylalanine (L-DOPA). The second reaction, diphenolase, oxidizes L-DOPA to L-dopaquinone ( Figure 1) [4]. The catalytic study of TYR has been studied with various substrates to measure monophenolase and diphenolase activities [5]. In this study, L-DOPA was used as the substrate to investigate the inhibition kinetics of various plant extracts during dopachrome production. has been studied with various substrates to measure monophenolase and diphen tivities [5]. In this study, L-DOPA was used as the substrate to investigate the in kinetics of various plant extracts during dopachrome production. While synthetic TYR inhibitors developed based on the catalytic mechanism hibit strong inhibition, these compounds also often establish cytotoxic and muta fects against mammalian cells [6]. At the same time, most TYR inhibitors exhibit anin biosynthesis of melanocytes fail clinical trials because of toxicity and low This is in part due to non-specific inhibition by the TYR inhibitors. According specific inhibitors need to be discovered, such as uncompetitive inhibitors that a TYR·L-DOPA complex. In the case of rare uncompetitive inhibition, the inhibit only to the ES complex resulting in lower apparent Km (Michaelis constant) and V imum reaction rate) when compared to the TYR activity without an inhibitor, an concentration of substrate cannot overcome the inhibition [7][8][9].
Inhibitors from natural resources, such as plant extracts, often suffer from non-specific inhibition. Besides, the vagueness of the inhibition mechanism of the greatly hinders practical application, regardless of many advantages, such as l direct production with less formulation, and safety [10]. Regardless, searching th tial TYR inhibitors from natural resources with low toxicity is continuously pe even with a low success rate, and alternative methods making up for such a draw still sought [2]. Therefore, we have investigated the inhibition of TYR by selected plant ex adopting a TYR inhibition kinetic study. In detail, high phenolic plants consu Asian people in their daily diet, including Panax ginseng (ginseng rhizomes and Alpinia galanga (galanga rhizomes and leaves) Vitis vinifera (grape seeds) and Mori era (moringa leaves), were selected. Along with total phenolic contents, TYR in and inhibition kinetics were studied to determine the TYR inhibition type, and th tive compound in the extract of the uncompetitive inhibitor was further investi HPLC-MS and detailed inhibition kinetics. While synthetic TYR inhibitors developed based on the catalytic mechanism can exhibit strong inhibition, these compounds also often establish cytotoxic and mutagenic effects against mammalian cells [6]. At the same time, most TYR inhibitors exhibiting melanin biosynthesis of melanocytes fail clinical trials because of toxicity and low activity. This is in part due to non-specific inhibition by the TYR inhibitors. Accordingly, more specific inhibitors need to be discovered, such as uncompetitive inhibitors that act on the TYR·L-DOPA complex. In the case of rare uncompetitive inhibition, the inhibitor binds only to the ES complex resulting in lower apparent K m (Michaelis constant) and V max (maximum reaction rate) when compared to the TYR activity without an inhibitor, and a high concentration of substrate cannot overcome the inhibition [7][8][9].
Inhibitors from natural resources, such as plant extracts, often suffer from low and non-specific inhibition. Besides, the vagueness of the inhibition mechanism of the extracts greatly hinders practical application, regardless of many advantages, such as low cost, direct production with less formulation, and safety [10]. Regardless, searching the potential TYR inhibitors from natural resources with low toxicity is continuously performed even with a low success rate, and alternative methods making up for such a drawback are still sought [2]. Therefore, we have investigated the inhibition of TYR by selected plant extracts by adopting a TYR inhibition kinetic study. In detail, high phenolic plants consumed by Asian people in their daily diet, including Panax ginseng (ginseng rhizomes and leaves), Alpinia galanga (galanga rhizomes and leaves) Vitis vinifera (grape seeds) and Moringa oleifera (moringa leaves), were selected. Along with total phenolic contents, TYR inhibition and inhibition kinetics were studied to determine the TYR inhibition type, and the bioactive compound in the extract of the uncompetitive inhibitor was further investigated by HPLC-MS and detailed inhibition kinetics.

TPC and TYR Inhibition
Polyphenolics are a group of plant secondary metabolites synthesized by shikimate and polyketide pathways. Many of these compounds with catechol or resorcinol functional groups, such as flavonoids, were known to inhibit TYR activity [11], and are resistant to intestinal metabolism by gut microbiota [12][13][14]. In this study, 70% ethanol was used to enrich the polyphenolic compounds from the plant samples. In the case of A. galanga, 95% ethanol was instead used, due to the high concentration of hydrophobic composition. Table 1 shows TPC of each extract expressed in mg of GAE/g of dry weight extract. The highest concentration of phenolic content was found in A. galanga rhizome BL (651.9 ± 4.2 mg), followed by M. oleifera leaf (472.4 ± 2.8 mg) and A. galanga leaf (448.1 ± 2.3 mg). P. ginseng leaf showed lowest phenolic content among the tested plants. All plant extracts inhibited TYR diphenolase activity in a dose-dependent manner, and the results were reported in terms of IC 50 ( Table 1). The extracts of A. galanga rhizome BL, M. oleifera leaf, and V. vinifera seed showed strong inhibition on TYR diphenolase, and the IC 50 values were found at 105.4 ± 0.2 µg/mL, 121.3 ± 0.4 µg/mL, and 94.8 ± 0.2 µg/mL, respectively. They showed stronger TYR diphenolase inhibition than ascorbic acid and kojic acid, of which the IC 50 were 235.7 ± 1.0 µg/mL and 192.3 ± 0.4 µg/mL, respectively. Strong TYR inhibition by A. galanga extract seems to be due to the high contents of curcuminoids, because curcuminoids inhibit proliferation of human melanocytes [15]. Additionally, industrial applications of grape seeds have recently been increasing due to the high content of health-promoting phytochemicals [16]; this reported TYR inhibition would further valorize its applications.
The correlation between TPC and IC 50 of TYR inhibition was determined by Pearson coefficient (bivariate) correlation. The result showed moderate correlation (r = −0.79, p < 0.05), which means there is a good negative correlation between TPC and IC 50 values ( Figure 2). The result implies that the inhibition on TYR diphenolase activity could be mainly due to the polyphenolic compounds in the extracts.

Kinetics Study of TYR Inhibition by A. galanga BL, M. oleifera and V. vinifera
The extracts of the A. galanga rhizome BL, M. oleifera leaf and V. vinifera seed with low IC50 values were selected for the kinetics study of TYR inhibition. Catalytic studies using L-DOPA substrate are known to follow the Michaelis-Menten model under aerobic conditions [5]. Kinetic parameters of Km and Vmax were determined as 0.41 ± 0.20 mg/mL (2.0 mM) and 1.01 ± 0.24 OD492/min, respectively, for the diphenolase activity of mushroom TYR used for the experiments. The Km value obtained from our assay system was similar to the reported value [17]. The slopes and y-intercepts in the Lineweaver-Burk plot obtained in the presence of various concentrations of the inhibitor were used to determine the inhibition constants of Ki and Kii (Table 2). The inhibition kinetics of A. galanga rhizome BL on TYR diphenolase activity at different concentrations of the extract exhibited mixed-type inhibition as shown in Figure  3A. The Lineweaver-Burk plots at different concentrations of the inhibitors did not intersect on either x or y axis. The inhibition constants of A. galanga zhizome BL were determined from the secondary plots ( Figure 3B,C). The intercept on the x-axis in Figure 3B plotted between the inhibitor concentration and the slope of each line in Figure 3A, determined Ki as 200 µg/mL. Similarly, the inhibition constant of Kii was determined as 128 µg/mL from Figure 3C, plotted between the inhibitor concentration and the intercept of each line in Figure 3A. Based on the inhibition constants, TYR diphenolase inhibition by A. galanga rhizome was stronger for the ES complex. Likewise, the inhibition by V. vinifera seed was also found as mixed-type ( Figure 4). Inhibition constants of Ki and Kii were determined as 42 µg/mL and 68 µg/mL, respectively. Strong inhibition by the extract of V. vinifera seeds may require further investigation. However, the mixed-type inhibition by

Kinetics Study of TYR Inhibition by A. galanga BL, M. oleifera and V. vinifera
The extracts of the A. galanga rhizome BL, M. oleifera leaf and V. vinifera seed with low IC 50 values were selected for the kinetics study of TYR inhibition. Catalytic studies using L-DOPA substrate are known to follow the Michaelis-Menten model under aerobic conditions [5]. Kinetic parameters of K m and V max were determined as 0.41 ± 0.20 mg/mL (2.0 mM) and 1.01 ± 0.24 OD 492 /min, respectively, for the diphenolase activity of mushroom TYR used for the experiments. The K m value obtained from our assay system was similar to the reported value [17]. The slopes and y-intercepts in the Lineweaver-Burk plot obtained in the presence of various concentrations of the inhibitor were used to determine the inhibition constants of K i and K ii ( Table 2). The inhibition kinetics of A. galanga rhizome BL on TYR diphenolase activity at different concentrations of the extract exhibited mixed-type inhibition as shown in Figure 3A. The Lineweaver-Burk plots at different concentrations of the inhibitors did not intersect on either x or y axis. The inhibition constants of A. galanga zhizome BL were determined from the secondary plots ( Figure 3B,C). The intercept on the x-axis in Figure 3B, plotted between the inhibitor concentration and the slope of each line in Figure 3A, determined K i as 200 µg/mL. Similarly, the inhibition constant of K ii was determined as 128 µg/mL from Figure 3C, plotted between the inhibitor concentration and the intercept of each line in Figure 3A. Based on the inhibition constants, TYR diphenolase inhibition by A. galanga rhizome was stronger for the ES complex. Likewise, the inhibition by V. vinifera seed was also found as mixed-type (Figure 4). Inhibition constants of K i and K ii were determined as 42 µg/mL and 68 µg/mL, respectively. Strong inhibition by the extract of V. vinifera seeds may require further investigation. However, the mixed-type inhibition by both extracts was not unexpected, considering the complex effects on TYR by the various compounds in the plant extracts.   Of significance, uncompetitive inhibition on TYR diphenolase was found from the M. aleifera leaf as shown at Figure 5. The Lineweaver-Burk plots at Figure 5A showed parallel lines, and Kii value of 73 µg/mL was determined from the secondary plot (Figure Of significance, uncompetitive inhibition on TYR diphenolase was found from the M. aleifera leaf as shown at Figure 5. The Lineweaver-Burk plots at Figure 5A showed parallel lines, and K ii value of 73 µg/mL was determined from the secondary plot ( Figure 5B). Considering the potent synthetic uncompetitive TYR inhibitors that were reported within a few µM of K ii , M. aleifera leaf is considered a very strong natural TYR inhibitor [18]. Uncompetitive inhibition is rare because it does not interact with the free enzyme, but only inhibits the ES complex. The uncompetitive inhibition and very low K ii value determined from the extract of M. oleifera leaf strongly suggested the presence of a potent TYR diphenolase inhibitor in the mixture, which specifically inhibited the ES complex.
Molecules 2021, 26, x FOR PEER REVIEW 7 of 12 within a few µM of Kii, M. aleifera leaf is considered a very strong natural TYR inhibitor [18]. Uncompetitive inhibition is rare because it does not interact with the free enzyme, but only inhibits the ES complex. The uncompetitive inhibition and very low Kii value determined from the extract of M. oleifera leaf strongly suggested the presence of a potent TYR diphenolase inhibitor in the mixture, which specifically inhibited the ES complex.

Compositional Analysis of M. oleifera Leaf Extract
The phytochemical composition of the M. oleifera leaf was investigated to search the active components responsible for the strong uncompetitive inhibition of TYR diphenolase. Among the major compounds identified from HPLC-MS analysis, (Figure 6, Supplementary Material), luteolin and its glucosides, luteolin 7-methyl glucuronide and luteolin 7-(6′′′-acetylallosyl-(1→2)-glucoside), drew out attention, because luteolin was identified as a major component of the extract (1.6%, Supplementary Material) and was reported as an uncompetitive TYR inhibitor [19]. In order to confirm the unique uncompetitive inhibition property of M. oleifera extract, the TYR inhibitory kinetics of luteolin was further investigated (Figure 7). The inhibition constant of Kii was found to be 33 µg/mL (113 µM) for the inhibition of TYR diphenolase activity by luteolin ( Table 2). The determined inhibition constant Kii was comparable to the reported value of 103 µM [19], but lower than that of M. oleifera leaf extract (73 µg/mL). Apparently, the extract of M. oleifera contains

Compositional Analysis of M. oleifera Leaf Extract
The phytochemical composition of the M. oleifera leaf was investigated to search the active components responsible for the strong uncompetitive inhibition of TYR diphenolase. Among the major compounds identified from HPLC-MS analysis, (Figure 6, Supplementary Material), luteolin and its glucosides, luteolin 7-methyl glucuronide and luteolin 7-(6acetylallosyl-(1→2)-glucoside), drew out attention, because luteolin was identified as a major component of the extract (1.6%, Supplementary Material) and was reported as an uncompetitive TYR inhibitor [19]. In order to confirm the unique uncompetitive inhibition property of M. oleifera extract, the TYR inhibitory kinetics of luteolin was further investigated (Figure 7). The inhibition constant of K ii was found to be 33 µg/mL (113 µM) for the inhibition of TYR diphenolase activity by luteolin ( Table 2). The determined inhibition constant K ii was comparable to the reported value of 103 µM [19], but lower than that of M. oleifera leaf extract (73 µg/mL). Apparently, the extract of M. oleifera contains other bioactive compounds that may interfere with TYR catalysis. Furthermore, the extract contained only 1.6% of luteolin. Therefore, we propose the glycosides of luteolin, such as luteolin 7-methyl glucuronide and luteolin 7-(6 -acetylallosyl-(1→2)-glucoside), can also be uncompetitive TYR diphenolase inhibitors, based on the HPLC-MS analysis. other bioactive compounds that may interfere with TYR catalysis. Furthermore, the extract contained only 1.6% of luteolin. Therefore, we propose the glycosides of luteolin, such as luteolin 7-methyl glucuronide and luteolin 7-(6′′′-acetylallosyl-(1→2)-glucoside), can also be uncompetitive TYR diphenolase inhibitors, based on the HPLC-MS analysis.  other bioactive compounds that may interfere with TYR catalysis. Furthermore, the extract contained only 1.6% of luteolin. Therefore, we propose the glycosides of luteolin, such as luteolin 7-methyl glucuronide and luteolin 7-(6′′′-acetylallosyl-(1→2)-glucoside), can also be uncompetitive TYR diphenolase inhibitors, based on the HPLC-MS analysis.

Discussion
Activity-guided isolation of bioactive compounds from natural resources has been a popular method for the development of value-added products, including medicines, functional foods, and cosmetics. In this study, we aimed to search an alternative methodology in the screening of TYR diphenolase inhibitors from the widely consumed plant extracts. Aas long as effective uncompetitive inhibition is screened, investigation of the enzyme inhibition kinetics of plant mixtures can be an efficient approach for the development of specific TYR inhibitors due to the guaranteed safety of plant extracts.
The results showed that M. oleifera, V. vinifera and A. galanga exhibited strong inhibition against TYR diphenolase, even compared with ascorbic acid and kojic acid, which are renowned TYR inhibitors. Further inhibition kinetics investigation was applied to elucidate the inhibition mechanism of the plant extracts. The observed mixed-type inhibition by V. vinifera and A. galanga implied that the compounds in the extract act non-specifically on both the free enzyme and ES complex at low concentrations. Interestingly, the uncompetitive modes of inhibition by M. oleifera extract strongly suggested specific ES complex inhibition by unidentified bioactive compounds. HPLC-MS analysis provided the phytochemical composition of M. oleifera, and luteolin and its glycosides were proposed as uncompetitive inhibitors. The inhibition by luteolin and M. oleifera in the presence of non-ionic detergent, triton X-100, was also performed to confirm that these are not Pan-Assay Interfering Substances (PAINS) [20]. The result has confirmed that they are specific uncompetitive inhibitors of TYR diphenolase (Supplementary Material). The rate-limiting step of TYR diphenolase was known to be the reaction step between L-DOPA and oxy-TYR [3]; hence, it appears luteolin and its glycosides inhibit the oxy-TYR·L-DOPA complex during the catalytic reaction.

Plant Materials and Extraction
Leaves and rhizomes of M. oleifera, A. galanga and P. ginseng were collected from local gardens in Khon Kaen, Thailand, during October 2017, and V. vinifera seeds were provided by Visootha (Nikki) Lohinavy, Gran Monte Asoke Valley in Pak Chong, Nakorn Rachasima, Thailand. Clean plant materials were dried by hot air oven at 65 • C (FD240, Binder, Germany) and powdered. All plant samples were extracted with 70% ethanol in a 1:4 w/v ratio, except A. galanga rhizomes which was extracted by 95% ethanol in the same ratio of sample to solvent. The mixture was stirred for 8 h at room temperature, and then filtrated by Whatman filter paper no. 1. All extracts were concentrated by rotary evaporator (Heidolph, VV2000, Schwabach, Germany). A. galanga rhizomes extract resulted in two layers, an oily yellow upper layer (YL) and brown lower layer (BL). All extracts were stored at 2 • C.

Total Phenolic Content
Total phenolic content (TPC) was measured by the Folin-Ciocalteu method [21]. Gallic acid with different concentrations of 50, 150, 250, and 500 mg/L was used for the construction of the standard curve. The reaction mixture was prepared by mixing 20 µL of sample (10 mg/mL in DMSO) with 1.58 mL of deionized water and 100 µL of Folin-Ciocalteu reagent. After incubating the reaction mixture at 25 • C for 5 min, 300 µL of 20% sodium carbonate was added. The absorbance was determined by spectrophotometer (2501PC, Shimadzu, Japan) at 765 nm. The TPC concentration was expressed as milligrams of gallic acid equivalent (GAE) per gram of dry extract.
Pearson coefficient (bivariate) correlation was determined by using the following equation, where r = correlation coefficient, xi = TPC, x = mean of xi, yi = IC 50 , and y = mean of yi.

Tyrosinase Inhibition
TYR inhibition was detected by the inhibition of the L-DOPA conversion into the colored dopachrome according to the published method [22]. Briefly, different concentrations of each plant extract were added into 96-well plates with 50 µL of mushroom TYR (300 U/mL in 0.1 mM phosphate buffer (PBS), pH 6.8), and the volume was adjusted to 200 µL with PBS (pH 6.8). The reaction mixture was incubated at 37 • C for 30 min. After that, 40 µl of L-DOPA (15 mM in 0.1 M PBS pH 6.8) was added to each well and incubated at 37 • C for 30 min in a dark place. Absorbance was recorded by microplate reader spectrophotometer (Imark, Biorad) at 492 nm. Kinetic parameters of K m and V max for TYR diphenolase activity with L-DOPA were determined by using the Lineweaver-Burk equation: 1 The concentration of plant extract which inhibited 50% enzyme activity was determined as IC 50 . All studies were conducted in triplicates and compared statistically. Kojic acid and ascorbic acid were used as the reference for TYR inhibitors.

Kinetic Study of TYR Inhibition
The extracts of A. galanga rhizome BL (50, 100 and 150 µg/mL), V. vinifera (25, 50 and 100 µg/mL), M. oleifera (125, 250 and 375 µg/mL), as well as luteolin (50, 75 and 100 µg/mL), were selected to determine the type of inhibition by Lineweaver-Burk plots. Each extract was added to the reaction mixture of 0.5-5.0 mg/mL of L-DOPA and 100 µL of 0.1 mM PBS (pH 6.8). The reaction was initiated by adding 50 µL of mushroom TYR at 37 • C. After incubation for 30 min, the absorbance was recorded by microplate reader spectrophotometer at 492 nm. The kinetic parameters of K m and V max were assessed from the original simulation dataset, using a Lineweaver-Burk plot of data obtained from different L-DOPA concentrations. The inhibition constant of K i (inhibition constant for free enzyme) was determined from the concentrations of the extract and the slopes of the Lineweaver-Burk plot by using the equation: slope = K M V max 1 + [I} K i , and K ii (inhibition constant for ES complex) was determined from the concentrations of the extract and the y-intercepts of the Lineweaver-Burk plot by using the equation:
The HPLC peaks were identified by using the spectrum database for organic compounds in the METLIN database.
Supplementary Materials: The following are available online. Figure S1: Mass spectrum of six components of Moringa oleifera leaf extract, Figure S2: HPLC chromatogram of (a) Luteolin pure compound, (b) Moringa oleifera leaves extract.