Synthesis and Insecticidal Evaluation of Chiral Neonicotinoids Analogs: The Laurel Wilt Case

Xyleborus sp beetles are types of ambrosia beetles invasive to the United States and recently also to Mexico. The beetle can carry a fungus responsible for the Laurel Wilt, a vascular lethal disease that can host over 300 tree species, including redbay and avocado. This problem has a great economic and environmental impact. Indeed, synthetic chemists have recently attempted to develop new neonicotinoids. This is also due to severe drug resistance to “classic” insecticides. In this research, a series of neonicotinoids analogs were synthesized, characterized, and evaluated against Xyleborus sp. Most of the target compounds showed good to excellent insecticidal activity. Generally, the cyclic compounds also showed better activity in comparison with open-chain compounds. Compounds R-13, 23, S-29, and 43 showed a mortality percent of up to 73% after 12 h of exposure. These results highlight the enantioenriched compounds with absolute R configuration. The docking results correlated with experimental data which showed both cation-π interactions in relation to the aromatic ring and hydrogen bonds between the search cavity 3C79 and the novel molecules. The results suggest that these sorts of interactions are responsible for high insecticidal activity.


Introduction
The redbay ambrosia beetle, Xyleborus glabratus, is the vector of the laurel wilt disease fungal pathogen, Raffaellea lauricola. Since the vector's initial detection in the USA in the early 2000s, laurel wilt has killed millions of redbay, Persea borbonia, trees, and other members of the plant family Lauraceae [1]. In this context, avocado (Persea Americana Mill.) is the most important agricultural crop susceptible to laurel wilt [2]. As the disease continues to move south and west from its original focus, it has caused significant concern in Florida, California, and other avocado-producing areas such as western Mexico. In the absence of effective control measures, monetary losses caused by laurel wilt could eventually range up to USD 54 million in the USA, and greater losses might occur if the disease moves elsewhere [3].
To the best of our knowledge, management is currently focused on monitoring, sanitation and direct control using contact or systemic insecticides [4], and these are still subject To the best of our knowledge, management is currently focused on monitoring, sanitation and direct control using contact or systemic insecticides [4], and these are still subject to ongoing investigations [5]. Indeed, efficacious and cost-effective measures are urgently needed to protect avocado from laurel wilt. In this context, neonicotinoids are an important type of compound with potent insecticidal activity. Since their introduction in the 1980s, these compounds have been established as the insecticides of choice for agricultural, animal health, and public health usages [6,7]. They are a type of insecticide that act selectively on the central nervous system of the insect and can be efficient ligands for the nicotinic acetylcholine receptors (nAChRs) of insects [8]. However, with the increase in neonicotinoids used for crop protection over a long time period, the problems of crossresistance [9], and bee toxicity [10] have received more attention, and this calls for a new strategy of molecular design to find new leading compounds. Neonicotinoids act selectively on the insect central nervous system (CNS) as an agonist of the postsynaptic nicotinic acetylcholine receptors (nAChRs) [11][12][13]. The great attributes of neonicotinoids are their novel mode of action, low mammalian toxicity, broad insecticidal spectrum, and good systemic properties [14]. Another important property of neonicotinoids is their environmental footprint, which allows for the replacement of the more toxic and non-selective organophosphorus, pyrethroid, and carbamate insecticides. The reported neonicotinoids can be classified according to the pharmacophore as N-nitroguanidines, (imidacloprid, thiamethoxam, clothianidin, and dinotefuran), nitromethylenes (nitenpyram), and N-cyano-amidines (acetamiprid and thiacloprid) ( Figure 1). All these compounds are characterized by their high insecticidal activities against insects and relative safety toward mammals and aquatic life [15]. Encouraged by these reports, we envisaged that incorporating different substituents into dinotefuran by switching the heterocyclic ring with different chiral enantioenriched and non-chiral amines could improve their insecticidal activity. Therefore, in a search for improvement, neonicotinoids with enantiomers of chiral amines were designed, synthesized, and evaluated. All these compounds are characterized by their high insecticidal activities against insects and relative safety toward mammals and aquatic life [15]. Encouraged by these reports, we envisaged that incorporating different substituents into dinotefuran by switching the heterocyclic ring with different chiral enantioenriched and non-chiral amines could improve their insecticidal activity. Therefore, in a search for improvement, neonicotinoids with enantiomers of chiral amines were designed, synthesized, and evaluated.

Synthesis
The starting material one was prepared using 1 equivalent of S-methylisothiourea hemisulfate salt in the presence of nitric (10 mL) and sulfuric acids (10 mL) at 0 • C for Molecules 2021, 26,4225 3 of 20 6 h (Scheme 1). Then, compound one was used to prepare the non-cyclic nitroguanidines 2-17. After dissolving the corresponding amine in chloroform, compound one was added in one portion. Most of the target compounds were purified through flash column chromatography in yields of up to 95%.

Synthesis
The starting material one was prepared using 1 equivalent of S-methylisothiourea hemisulfate salt in the presence of nitric (10 mL) and sulfuric acids (10 mL) at 0 °C for 6 h (Scheme 1). Then, compound one was used to prepare the non-cyclic nitroguanidines 2-17. After dissolving the corresponding amine in chloroform, compound one was added in one portion. Most of the target compounds were purified through flash column chromatography in yields of up to 95%. Scheme 1. Preparation of starting material 1.
Sixteen non-cyclic nitroguanidines were prepared and these produced different substitution patterns. For instance, nitroguanidines 2 and 3 had heterocyclic rings while 4 to 10 had an aromatic moiety with electron-donating and withdrawing substituents. Enantiopure non-cyclic nitroguanidines were also prepared using different sorts of chiral amines such as R-and S-phenylethylamine, and napthylethylamine, S-cyclopropylethylamine, and R-cyclohexylethylamine (Scheme 2). All these reactions proceeded through nucleophilic substitution: the lone pair of electrons on the amine attacked the sp 2 carbon of compound one, then the -SCH3 moiety was displaced to obtain the desired nitroguanidine. Scheme 3 shows that cyclic nitroguanidines were prepared using 6 mL of the solution of formaldehyde and formic acid (1:1) and the corresponding nitroguanidine. The mixture was heated at 90 °C for 6 h. Most of the compounds were obtained from moderate to good yields. Sixteen non-cyclic nitroguanidines were prepared and these produced different substitution patterns. For instance, nitroguanidines 2 and 3 had heterocyclic rings while 4 to 10 had an aromatic moiety with electron-donating and withdrawing substituents. Enantiopure non-cyclic nitroguanidines were also prepared using different sorts of chiral amines such as Rand S-phenylethylamine, and napthylethylamine, S-cyclopropylethylamine, and R-cyclohexylethylamine (Scheme 2). All these reactions proceeded through nucleophilic substitution: the lone pair of electrons on the amine attacked the sp 2 carbon of compound one, then the -SCH 3 moiety was displaced to obtain the desired nitroguanidine.

Synthesis
The starting material one was prepared using 1 equivalent of S-methylisothiourea hemisulfate salt in the presence of nitric (10 mL) and sulfuric acids (10 mL) at 0 °C for 6 h (Scheme 1). Then, compound one was used to prepare the non-cyclic nitroguanidines 2-17. After dissolving the corresponding amine in chloroform, compound one was added in one portion. Most of the target compounds were purified through flash column chromatography in yields of up to 95%. Scheme 1. Preparation of starting material 1.
Sixteen non-cyclic nitroguanidines were prepared and these produced different substitution patterns. For instance, nitroguanidines 2 and 3 had heterocyclic rings while 4 to 10 had an aromatic moiety with electron-donating and withdrawing substituents. Enantiopure non-cyclic nitroguanidines were also prepared using different sorts of chiral amines such as R-and S-phenylethylamine, and napthylethylamine, S-cyclopropylethylamine, and R-cyclohexylethylamine (Scheme 2). All these reactions proceeded through nucleophilic substitution: the lone pair of electrons on the amine attacked the sp 2 carbon of compound one, then the -SCH3 moiety was displaced to obtain the desired nitroguanidine. Scheme 3 shows that cyclic nitroguanidines were prepared using 6 mL of the solution of formaldehyde and formic acid (1:1) and the corresponding nitroguanidine. The mixture was heated at 90 °C for 6 h. Most of the compounds were obtained from moderate to good yields. Scheme 3 shows that cyclic nitroguanidines were prepared using 6 mL of the solution of formaldehyde and formic acid (1:1) and the corresponding nitroguanidine. The mixture was heated at 90 • C for 6 h. Most of the compounds were obtained from moderate to good yields. The reaction proceeded through the Mannich-type reaction; here, the acid was used as a catalyst to promote the reaction where the key intermediate was the diol, which was then cyclized to obtain the corresponding cyclic nitroguanidine (Scheme 4) [16]. To obtain the N-methylated compounds 33−42, compound 32 was synthesized using phthaloyl chloride, pyridine, and a solution of HCl/H2O. The resulting precipitate was filtered and recrystallized from EtOH to obtain the heterocyclic compound (Scheme 5). Afterwards, a stirred solution of 32 in acetonitrile was added dropwise to a solution of the corresponding amine at 0 °C. Then, the reaction was stirred for 30 min at the same temperature and a 2.0 M solution of methylamine in MeOH was immediately added. Most of the compounds were obtained with good yields (Scheme 6). It is important to point out that the reactions were carried out in one-pot. The addition of the amine and the addition of the methylamine were nucleophilic substitutions that took place at the sp 2 carbon. The reaction proceeded through the Mannich-type reaction; here, the acid was used as a catalyst to promote the reaction where the key intermediate was the diol, which was then cyclized to obtain the corresponding cyclic nitroguanidine (Scheme 4) [16]. The reaction proceeded through the Mannich-type reaction; here, the acid was used as a catalyst to promote the reaction where the key intermediate was the diol, which was then cyclized to obtain the corresponding cyclic nitroguanidine (Scheme 4) [16]. To obtain the N-methylated compounds 33−42, compound 32 was synthesized using phthaloyl chloride, pyridine, and a solution of HCl/H2O. The resulting precipitate was filtered and recrystallized from EtOH to obtain the heterocyclic compound (Scheme 5). Afterwards, a stirred solution of 32 in acetonitrile was added dropwise to a solution of the corresponding amine at 0 °C. Then, the reaction was stirred for 30 min at the same temperature and a 2.0 M solution of methylamine in MeOH was immediately added. Most of the compounds were obtained with good yields (Scheme 6). It is important to point out that the reactions were carried out in one-pot. The addition of the amine and the addition of the methylamine were nucleophilic substitutions that took place at the sp 2 carbon. To obtain the N-methylated compounds 33-42, compound 32 was synthesized using phthaloyl chloride, pyridine, and a solution of HCl/H 2 O. The resulting precipitate was filtered and recrystallized from EtOH to obtain the heterocyclic compound (Scheme 5). Afterwards, a stirred solution of 32 in acetonitrile was added dropwise to a solution of the corresponding amine at 0 • C. Then, the reaction was stirred for 30 min at the same temperature and a 2.0 M solution of methylamine in MeOH was immediately added. Most of the compounds were obtained with good yields (Scheme 6). It is important to point out that the reactions were carried out in one-pot. The addition of the amine and the addition of the methylamine were nucleophilic substitutions that took place at the sp 2 carbon. To synthesize compounds 43 to 47, the same procedure described for compounds 18 to R-31 was followed. Again, these reactions proceeded through a Mannich-type reaction. The five products were obtained in yields of up to 71% (Scheme 7). To synthesize compounds 43 to 47, the same procedure described for compounds 18 to R-31 was followed. Again, these reactions proceeded through a Mannich-type reaction. The five products were obtained in yields of up to 71% (Scheme 7). To synthesize compounds 43 to 47, the same procedure described for compounds 18 to R-31 was followed. Again, these reactions proceeded through a Mannich-type reaction. The five products were obtained in yields of up to 71% (Scheme 7).

Biological Activities
The insecticidal assays of the title compounds against Xyleborus affinis were tested using the filter paper contact toxicity method. The results were graphed for comparison. Most of the non-cyclic compounds showed a lower insecticidal activity than dinotefuran and the chiral and enantiopure compounds such as

Biological Activities
The insecticidal assays of the title compounds against Xyleborus affinis were tested using the filter paper contact toxicity method. The results were graphed for comparison. Most of the non-cyclic compounds showed a lower insecticidal activity than dinotefuran and the chiral and enantiopure compounds such as (R,E)-1-(cyclohexylethyl)-2-nitroguanidine R-13, which showed up to 67% mortality ( Figure 2). Scheme 7. N-methylated cyclic nitroguanidines.

Biological Activities
The insecticidal assays of the title compounds against Xyleborus affinis were tested using the filter paper contact toxicity method. The results were graphed for comparison. Most of the non-cyclic compounds showed a lower insecticidal activity than dinotefuran and the chiral and enantiopure compounds such as (R,E)-1-(cyclohexylethyl)-2-nitroguanidine R-13, which showed up to 67% mortality ( Figure 2). The cyclic nitroguanidines 21, 23, R-24, R-28, and R-29 showed insecticidal activities of up to 73%. The comparison between nitroguanidines 21 and 23 suggest that compounds with an electron-donating group in the para position could have a higher mortality percent than compounds that have electron-withdrawing groups. It was also observed that The cyclic nitroguanidines 21, 23, R-24, R-28, and R-29 showed insecticidal activities of up to 73%. The comparison between nitroguanidines 21 and 23 suggest that compounds with an electron-donating group in the para position could have a higher mortality percent than compounds that have electron-withdrawing groups. It was also observed that enantiopure aliphatic nitroguanidines, R-28 and S-29, have a good insecticidal activity up to 73% ( Figure 3).  The analysis of non-cyclic and cyclic N-methylated nitroguanidines allows us to highlight the compounds 36, 43, and 47. The structure-activity relationship suggests again that compounds with electron-donating or electron-withdrawing groups at the para position of the aromatic ring have higher insecticidal activities ( Figure 4). The analysis of non-cyclic and cyclic N-methylated nitroguanidines allows us to highlight the compounds 36, 43, and 47. The structure-activity relationship suggests again that compounds with electron-donating or electron-withdrawing groups at the para position of the aromatic ring have higher insecticidal activities ( Figure 4). The analysis of non-cyclic and cyclic N-methylated nitroguanidines allows us to highlight the compounds 36, 43, and 47. The structure-activity relationship suggests again that compounds with electron-donating or electron-withdrawing groups at the para position of the aromatic ring have higher insecticidal activities ( Figure 4).  Figure 4. Analysis of (a) non-cyclic nitroguanidines; (b) cyclic and N-methylated nitroguanidines.

Structure-Activity Relationship (SAR) of the Para Halogenated Aromatic Derivatives
The SAR analysis showed that non-cyclic and N-methylated compounds had a higher insecticidal activity. On the other hand, compounds that incorporated the para electronwithdrawing group attached to the aromatic ring, such as CF 3 or Cl, had the same tendency in terms of insecticidal activity. As a matter of fact, the presence of a halogen at a specific position in the molecule can influence its biological activity [17]. The N-methylated compounds have a better insecticidal activity than non-methylated compounds (compound 21 and 8 vs. compound 9 and 20). In contrast to our results with neonicotinoids, Xu, et al. [18] designed and synthesized a series of 1,5-diphenyl-2-penten-1-one analogues with piperazine and evaluated their bioactivities. The SAR analysis showed that the R ring plays a crucial role, and that the antifungal activities and larvicide against mosquitoes of the compounds with N'-unsubstituted piperazine were better than those of the compounds with N'-methyl piperazine. Likewise, its larvicidal activity was severely decreased when the substituent on the benzene ring was changed from position four to position two or three. However, the improved biopotential of the N-methylated compounds studied in our work is in agreement with Dahiya et al. [19], who synthesized an N-methylated analog of a proline-rich cyclic tetracyclopeptide from marine bacteria with enhanced anthelmintic and antifungal activity, compared to the non-methylated tetracyclopeptide. Cyclic compounds showed up to 67% mortality for compound 36 and 47% mortality for compound 35 ( Figure 5). mosquitoes of the compounds with N'-unsubstituted piperazine were better than those of the compounds with N'-methyl piperazine. Likewise, its larvicidal activity was severely decreased when the substituent on the benzene ring was changed from position four to position two or three. However, the improved biopotential of the N-methylated compounds studied in our work is in agreement with Dahiya et al. [19], who synthesized an N-methylated analog of a proline-rich cyclic tetracyclopeptide from marine bacteria with enhanced anthelmintic and antifungal activity, compared to the non-methylated tetracyclopeptide. Cyclic compounds showed up to 67% mortality for compound 36 and 47% mortality for compound 35 ( Figure 5). It is well known that chirality plays an important role when bioactive enantiopure compounds are administered to living organisms [20]. As a part of our research program in relation to biologically active and enantioenriched compounds, we prepared both the enantiomers of nitroguanidines derivatives that had the phenylethylamine or the naphtyletylamine in enantiopure form. Those compounds were bioassayed and they showed interesting differences in their insecticidal activity. For instance, the non-cyclic R enantiomers showed a higher insecticidal activity. Moreover, the cyclic enantiomers with an absolute R configuration showed a higher insecticidal activity when compared with the S Figure 5. Structure-activity analysis of new neonicotinoids.
It is well known that chirality plays an important role when bioactive enantiopure compounds are administered to living organisms [20]. As a part of our research program in relation to biologically active and enantioenriched compounds, we prepared both the enantiomers of nitroguanidines derivatives that had the phenylethylamine or the naphtyletylamine in enantiopure form. Those compounds were bioassayed and they showed interesting differences in their insecticidal activity. For instance, the non-cyclic R enantiomers showed a higher insecticidal activity. Moreover, the cyclic enantiomers with an absolute R configuration showed a higher insecticidal activity when compared with the S enantiomers ( Figure 6). The favored insecticidal activity of the R enantiomers has previously been reported, for instance, in limonene against Tribolium confusum [21], in cycloprothrins against the larvae of Tetranychus cinnabarnus, Nilaparvatalegen, Mythimaseparata, and Aphismedicagini [22], and also in pyridine methanesulfonates against Diabrotica undecimpunctata howardi and Nephotettix cincticeps [23].

Docking Analysis
To perform the docking analysis, the highly bioactive compounds 47, R-13, 43, 23, 36, and S-29 were selected. First, the score of dinotefuran was obtained since this was our model insecticide. The score was −5.64 kcal/mol. The lower the score, the better the affinity with the receptor in the search cavity of 3C79. The compound with an outstanding affinity was the cyclic N-methylated compound 47 which has a p-methoxy-substituted aromatic ring. This compound was followed by the enantiopure compound R-13 that possessed an absolute R configuration. The results suggest that the non-methylated 23 and non-cyclic 36 compounds presented a diminished affinity. The compound with an absolute R configuration had a better affinity when compared with the S compound. All the docking calculations agreed with the experimental results. Another interesting observation derived from docking was that the inhibitions constants (Ki) were lower for compounds 47, R-13, and 43. For instance, the comparison between dinotefuran, R-13, and S-29, showed that these values were 24.23, 73.06, 263.75 μM, respectively. These results suggest again that compounds with absolute R configurations are more potent and improved inhibitors since the concentration to produce the half-maximum inhibition could be reduced by up to 33% in comparison with dinotefuran. The comparison of the Ki between compounds with opposite absolute configurations of R-13 and S-29 showed a huge difference since

Docking Analysis
To perform the docking analysis, the highly bioactive compounds 47, R-13, 43, 23, 36, and S-29 were selected. First, the score of dinotefuran was obtained since this was our model insecticide. The score was −5.64 kcal/mol. The lower the score, the better the affinity with the receptor in the search cavity of 3C79. The compound with an outstanding affinity was the cyclic N-methylated compound 47 which has a p-methoxy-substituted aromatic ring. This compound was followed by the enantiopure compound R-13 that possessed an absolute R configuration. The results suggest that the non-methylated 23 and non-cyclic 36 compounds presented a diminished affinity. The compound with an absolute R configuration had a better affinity when compared with the S compound. All the docking calculations agreed with the experimental results. Another interesting observation derived from docking was that the inhibitions constants (K i ) were lower for compounds 47, R-13, and 43. For instance, the comparison between dinotefuran, R-13, and S-29, showed that these values were 24.23, 73.06, 263.75 µM, respectively. These results suggest again that compounds with absolute R configurations are more potent and improved inhibitors since the concentration to produce the half-maximum inhibition could be reduced by up to 33% in comparison with dinotefuran. The comparison of the K i between compounds with opposite absolute configurations of R-13 and S-29 showed a huge difference since these values were 24.23 and 263.75, respectively ( Table 1). The experimental results and the docking results suggest again that compounds with an R configuration could have a highly insecticidal activity at lesser doses. Additionally, the in-silico calculations suggest that cyclic N-methylated compounds 47 and 43 are highly active insecticides against Xyleborus affinis. Table 1. Affinity and inhibition constants of highly bioactive compounds a .

Compound
Free Energy (kcal/mol) "Score" with the receptor in the search cavity of 3C79. The compound with an outstanding affinity was the cyclic N-methylated compound 47 which has a p-methoxy-substituted aromatic ring. This compound was followed by the enantiopure compound R-13 that possessed an absolute R configuration. The results suggest that the non-methylated 23 and non-cyclic 36 compounds presented a diminished affinity. The compound with an absolute R configuration had a better affinity when compared with the S compound. All the docking calculations agreed with the experimental results. Another interesting observation derived from docking was that the inhibitions constants (Ki) were lower for compounds 47, R-13, and 43. For instance, the comparison between dinotefuran, R-13, and S-29, showed that these values were 24.23, 73.06, 263.75 μM, respectively. These results suggest again that compounds with absolute R configurations are more potent and improved inhibitors since the concentration to produce the half-maximum inhibition could be reduced by up to 33% in comparison with dinotefuran. The comparison of the Ki between compounds with opposite absolute configurations of R-13 and S-29 showed a huge difference since these values were 24.23 and 263.75, respectively ( Table 1). The experimental results and the docking results suggest again that compounds with an R configuration could have a highly insecticidal activity at lesser doses. Additionally, the in-silico calculations suggest that cyclic N-methylated compounds 47 and 43 are highly active insecticides against Xyleborus affinis.  16.29 with the receptor in the search cavity of 3C79. The compound with an outstanding affinity was the cyclic N-methylated compound 47 which has a p-methoxy-substituted aromatic ring. This compound was followed by the enantiopure compound R-13 that possessed an absolute R configuration. The results suggest that the non-methylated 23 and non-cyclic 36 compounds presented a diminished affinity. The compound with an absolute R configuration had a better affinity when compared with the S compound. All the docking calculations agreed with the experimental results. Another interesting observation derived from docking was that the inhibitions constants (Ki) were lower for compounds 47, R-13, and 43. For instance, the comparison between dinotefuran, R- 13, and S-29, showed that these values were 24.23, 73.06, 263.75 μM, respectively. These results suggest again that compounds with absolute R configurations are more potent and improved inhibitors since the concentration to produce the half-maximum inhibition could be reduced by up to 33% in comparison with dinotefuran. The comparison of the Ki between compounds with opposite absolute configurations of R-13 and S-29 showed a huge difference since these values were 24.23 and 263.75, respectively ( Table 1). The experimental results and the docking results suggest again that compounds with an R configuration could have a highly insecticidal activity at lesser doses. Additionally, the in-silico calculations suggest that cyclic N-methylated compounds 47 and 43 are highly active insecticides against Xyleborus affinis. The 2D and 3D representations of the molecular recognition with the residues of the search cavity of the binding protein 3C79 (AChBP) with dinotefuran and the compounds selected to perform the docking showed different types of interactions among them (Figure 7). For instance, dinotefuran showed interactions between the nitrogen attached to a heterocyclic moiety and the receptor in Glu153 and Asp77. An interaction between the oxygen of the heterocyclic ring and the Thr24 of the receptor was also observed. The 2D and 3D representations of the molecular recognition with the residues of the search cavity of the binding protein 3C79 (AChBP) with dinotefuran and the compounds selected to perform the docking showed different types of interactions among them (Figure 7). For instance, dinotefuran showed interactions between the nitrogen attached to a heterocyclic moiety and the receptor in Glu153 and Asp77. An interaction between the oxygen of the heterocyclic ring and the Thr24 of the receptor was also observed. The 2D and 3D representations of the molecular recognition with the residues of the search cavity of the binding protein 3C79 (AChBP) with dinotefuran and the compounds selected to perform the docking showed different types of interactions among them (Figure 7). For instance, dinotefuran showed interactions between the nitrogen attached to a heterocyclic moiety and the receptor in Glu153 and Asp77. An interaction between the  The 2D and 3D representations of the molecular recognition with the residues of the search cavity of the binding protein 3C79 (AChBP) with dinotefuran and the compounds selected to perform the docking showed different types of interactions among them (Figure 7). For instance, dinotefuran showed interactions between the nitrogen attached to a heterocyclic moiety and the receptor in Glu153 and Asp77. An interaction between the a To perform the simulations, the parameters of 100 runs with 5 million evaluations were used for each molecular coupling experiment using a Lamarckian genetic algorithm.
The 2D and 3D representations of the molecular recognition with the residues of the search cavity of the binding protein 3C79 (AChBP) with dinotefuran and the compounds selected to perform the docking showed different types of interactions among them (Figure 7). For instance, dinotefuran showed interactions between the nitrogen attached to a heterocyclic moiety and the receptor in Glu153 and Asp77. An interaction between the The 2D and 3D representations of the molecular recognition with the residues of the search cavity of the binding protein 3C79 (AChBP) with dinotefuran and the compounds selected to perform the docking showed different types of interactions among them (Figure 7). For instance, dinotefuran showed interactions between the nitrogen attached to a heterocyclic moiety and the receptor in Glu153 and Asp77. An interaction between the oxygen of the heterocyclic ring and the Thr24 of the receptor was also observed. On the other hand, a cation-π interaction between the aromatic ring of compounds 47 and 43 and Phe78 was found. These sorts of interactions play an important role in neonicotinoid insecticidal activity [24]. The enantiopure compound 13 showed interactions with Glu153 and As77; in comparison with dinotefuran, these interactions appear to be due to hydrogen bonds and the results suggest that these sorts of interactions are responsible for the higher insecticidal activity. These results also suggest that this strategy could result in a useful tool to fight cross-resistance, since the nature of the interactions between the novel molecules and the receptor are quite different. Examples of intragroup cross-resistance are well documented for most major insecticide groups, including pyrethroids, organophosphate, and neonicotinoids [25][26][27]. However, cross-resistance can also be unpredictable, affecting compounds with diverse structures and those that are considered to have different modes of action.

Instrumentation and Chemicals
All reactions were carried out in flame-dried glassware with a nitrogen atmosphere and magnetic stirring unless otherwise noted. Analytical thin-layer chromatography (TLC) was performed on Merck pre-coated TLC plates (silica gel 60 GF254, 0.25 mm). Flash chromatography was performed on silica gel E. Merck 9385 or silica gel 60 extra pure (for final products). Dichloromethane (CH 2 Cl 2 ) and toluene (PhCH 3 ) were purchased from Sigma-Aldrich and were purified by distillation. Most of the chemicals were purchased from Aldrich, Stream, or Alfa Aesar and handled and stored in a desiccator. Molecular sieves were purchased from Sigma-Aldrich and activated with a microwave oven. All other reagents and starting materials, unless otherwise noted, were purchased from commercial vendors and used without further purification. Infrared (IR) spectra were recorded on an Agilent Technologies Cary 600 Series FTIR. 1 H, 13 C, DEPTQ 135, and bidimensional NMR spectra were recorded on a Bruker Avance III HD 500 spectrometer (Billerica, MA, USA). Chemical shift values (δ) are expressed in ppm downfield relative to an internal standard (tetramethylsilane at 0 ppm). Multiplicities are indicated as s (single), d (doublet), t (triplet), q (quartet), m (multiplet), and br s (broad signal). The solvent CDCl 3 at 77.16, DMSO-d 6 at 39.52, and CD 3 OD at 49 ppm were used as internal standards for 13 C NMR spectra. Spectra were processed using TopSpin 4.0.7 from Bruker BioSpin © (Billerica, MA, USA). The 1 H and 13 C NMR spectra for all compounds can be found in the Supplementary Materials. Optical rotations were measured on a Bellingham + Stanley ADP 440 + polarimeter (Kent, UK). High-resolution mass spectra (HRMS) were obtained in a Q-TOF mass spectrometer equipped with electrospray ionization (ESI) interface Synapt G2-Si, Waters Inc. (Milford, MA, USA). Melting points were not corrected and determined on a Stuart SMP10 apparatus (Staffordshire, UK) using open glass capillaries.

Synthetic Procedures
Unless otherwise noted, reagents and solvents were used as received from commercial suppliers. Yields were not optimized. The synthetic procedures have already been described in the literature and were followed to prepare the novel neonicotinoids.

Synthetic Procedure to Obtain the Methyl N-Carbamimidothioate 1
Concentrated sulfuric acid (10 mL) was placed in a balloon flask equipped with a stirring bar and a thermometer and it was cooled with an ice bath to 0 • C. When this temperature was reached, 70% nitric acid was added (10 mL). The cooling was maintained until the initial temperature of 0 • C was reached, and then 1 equivalent of S-methylisothiourea hemisulfate was added. The mixture was stirred for 6 h at 0 • C and once the reaction time had elapsed, the contents of the flask were poured over a beaker with ice to obtain a precipitate. This mixture was kept under refrigeration for 24 h to melt the remaining ice. The isolation of the product was achieved by filtration under reduced pressure and washing of the solid with cold distilled water. Finally, the solid was dried under a high vacuum to obtain 1 [28].

General Synthetic Procedure for Non-Cyclic Compounds 2-17
To a stirred solution of the corresponding amine (1.1 mmol) in chloroform (1 mL) compound 1 was added (1.0 mmol) in one portion, after that the reaction was stirred for 24 h at room temperature. The solvent was evaporated under reduced pressure and the crude was purified using flash column chromatography [29]. See Data Compounds in Appendix A.

General Synthetic Procedure for Cyclic Compounds 18-31
In a solution of formaldehyde (37%) and formic acid (1:1, 6 mL) the corresponding nitroguanidine (1 mmol) was suspended. The mixture was heated at 90 • C for 6 h. The reaction was extracted using AcOEt (3 × 10 mL). The organic phases were collected, filtered over Na 2 SO 4 , and evaporated. The crude was purified using flash column chromatography [16]. See Data Compounds in Appendix B.

Synthetic Procedure to Obtain the Intermediate 32
In a flask equipped with a stir bar, compound 1 (1 mmol) was dissolved in pyridine (1 mL) and cooled in an ice-water bath to 0 • C. Subsequently, 90% phthaloyl chloride solution (2 mmol) was added dropwise for 30 min. After the addition was completed, the reaction was stirred for an additional 10 min and at the end of that time, the mixture was poured over a 1:5 HCl/H 2 O solution (20 mL). The resulting precipitate was filtered under reduced pressure, recrystallized in EtOH, and dried under a high vacuum to obtain 32.

General Synthetic Procedure to Obtain N-Methylated Compounds
To a stirred solution of 32 (1 mmol) in acetonitrile (2 mL) a solution of the corresponding amine (1.1 mmol) in acetonitrile (1.0 mL) was added over 20 min at 0 • C. The reaction was stirred for 30 min at the same temperature and a 2.0 M solution of methylamine in MeOH (3.0 mmol) was added immediately. The mixture was stirred for 3 h at room temperature. The solvent was evaporated under reduced pressure and the crude was purified using flash column chromatography [29]. See Data Compounds in Appendix C.

Biological Assay
The insecticidal activities of the title compounds against Xyleborus affinis were tested according to the previously reported procedure of the contact toxicity on filter paper [30]: A Whatman TM (Buckinghamshire, UK) grade 42, ashless filter paper (5 cm diameter) was placed in a glass Petri dish (50 × 17 mm diameter). An aliquot of 0.25 mL of the solution 0.05 M of the compound in dimethylsulfoxide:water (1:1) was applied uniformly to the filter paper disc. The mixture of solvents was used as the negative control and the solution of dinotefuran at the same concentration was used as the positive control. The solvent was allowed to distribute evenly for 5 min prior to the introduction of 5 adult insects into each dish. Since the boiling point of dimethyl sulfoxide and water are high enough to be volatilized at room temperature, it was not necessary to replenish the solvent mixture. According to statistical requirements, each treatment was replicated 3 times at 25 • C ± 1 • C with the organism grown in the laboratory. Xyleborus affinis were reared in an artificial media according to Biedermann et al. [31], with some modification. Rearing media were maintained in a climatic chamber at 26 • C and 60% of RH in complete dark. Adults were obtained at 30 days after female inoculation by dissecting the media culture. Insect mortalities were recorded after 12 h. Insects were presumed dead if they remained immobile and did not respond to three probings with a blunt dissecting probe after a 5-min recovery period.

Conclusions
A series of novel neonicotinoid analogs were designed and synthesized by introducing aliphatic and aromatic amines. Interestingly, enantiopure amines with opposite absolute configurations were prepared and analyzed as insecticides against Xyleborus affinis. The results showed us that the amines with an absolute R configuration have a superior biological activity when compared to the opposite enantiomer S. Four lead compounds that had percent mortalities up to 73% were derived from this study, while dinotefuran had only a 40% mortality.