Photodegradation of Brilliant Green Dye by a Zinc bioMOF and Crystallographic Visualization of Resulting CO2

We present a novel bio-friendly water-stable Zn-based MOF (1), derived from the natural amino acid L-serine, which was able to efficiently photodegrade water solutions of brilliant green dye in only 120 min. The total degradation was followed by UV-Vis spectroscopy and further confirmed by single-crystal X-ray crystallography, revealing the presence of CO2 within its channels. Reusability studies further demonstrate the structural and performance robustness of 1.


Introduction
One of the main challenges that modern society faces is related, undoubtedly, to the contamination of aquatic environments, which is mainly caused by human/industrial activities [1,2]. Among the wide diversity of inorganic/organic chemical pollutants, organic dyes-waste generated in the cosmetic, textile, tannery, or food industries, among othersconstitute one of the major contaminants in industrial wastewater [3].
Proposed solutions for the removal of such organic contaminants include precipitation, coagulation/flocculation, membrane technology, or biological processes [4]. However, probably the two most promising technologies for this purpose are based on the straightforward capture of the organic dyes by a porous material or their in-situ photocatalytic degradation [5,6]. In particular, metal-organic frameworks (MOFs) are porous crystalline materials that have already been shown to be efficient in the last two approaches [7][8][9][10][11][12][13].
In particular, MOFs have recently been used in water remediation with exceptional results. For example, the easy of functionalizing, pre-or post-synthetically, MOF channels has led to the preparation of specifically designed MOFs for the selective and efficient capture of both organic and inorganic contaminants [8,[33][34][35]. Moreover, certain specifically designed MOFs have already been shown to be effective as photocatalysts to degrade organic pollutants-i.e., organic dyes-into less toxic intermediates or fully degrade them into CO 2 and H 2 O [10,[36][37][38][39][40]. In particular, several Zn(II)-based MOFs have recently been reported, showing, by analogy with zinc oxide based photocatalysts [41,42], moderately good efficiencies as photocatalysts towards different organic dyes [43][44][45].

Results
In this communication, we report the preparation and total characterization of a novel eco-friendly Zn-based MOF, derived from the natural amino acid L-serine, with formula {Zn II (Figure 1a,b). The final material is capable of photodegrading brilliant green (BG) dye ( Figure 1c) in only 120 min with an efficiency of 100% in the absence of any other oxidant or co-catalyst. In addition, the high robustness and crystallinity of 1 also allowed us to obtain the crystal structure of 1-with the help of SCXRD-after the photocatalytic process, which shows, unambiguously, the presence, within the channels, of CO 2 molecules resulting from the photodegradation of BG dyes.   Crystals of 1, left in a sealed glass tube containing an aqueous solution of BG dye for one week, after irradiation in the range 250-350 nm for 60 min were analyzed by SCXRD at a temperature of 100 K and the crystal structure of CO 2 @1 was determined, revealing a slight deformation of the framework-likely correlated to CO 2 adsorption process-but still isomorphous to 1, crystallizing in the P4 1 2 1 2 space group. Adsorption of CO 2 gasproduced by the photocatalytic process at 298 K-results in linear negative expansion. Pores that run parallel to the c-axis contract upon CO 2 inclusion (∆a, ∆b slightly < 0; ∆c < 0; ∆V < 0). The largest change in dimensions is observed for the c axis, accounting for a small decrease in the unit-cell volume (∆V ≈ 48 Å 3 , Table S1).
The crystal structure of the adsorbate CO 2 @1 clearly evidences the presence of CO 2 guest molecules hosted in the hydrophilic irregular pores of 1 (Figure 3b, Figures S4 and S6. A primary adsorption site can be identified on the CO 2 molecule (Figure 4), where the plane containing the ligands are situated at only 2.83(1) Å [distance between centroid of oxamate core and C atom of CO 2 molecule] from the C atom of CO 2 molecules occupying the primary site, suggesting an interaction between the C(δ + ) atom of the CO 2 molecule and the O lone pair of the oxamate core of the ligand, as observed before [46]. The described guest molecule orientation generates a kind of π(CO 2 )···π(oxamate) dimer exhibiting C(oxamate)···O(CO 2 ) and C(CO 2 )···O(oxamate) symmetric contacts of 3.08(1) and 3.32(1) Å, respectively. The secondary adsorption sites identified are situated at the center of the pores, where CO 2 molecules are packed with closest contact with the framework through the  What is worth underlining is the unusual penta-coordination of Zn(II) ions observed in 1 and CO 2 @1, reminiscent (in geometry) of that observed for the catalytic metal ions of the di-zinc aminopeptidase from Aeromonas proteolytica (AAP) [48]. Surveys of the Cambridge Structural Database (CSD) show zinc ion coordination number frequencies of ca. 60% and 25% for 4 and 6 coordination numbers, respectively. Interestingly, the zinc ion coordination prevalence in protein sites depends on whether the zinc plays a structural or a catalytic role. In structural zinc sites, the occurrence rate for 4, 5, and 6 coordination numbers is 80%, 6%, and 12%, respectively; whereas in catalytic zinc sites, the occurrence rate for 4, 5, and 6 coordination is 47%, 45%, and 6%, respectively [49]. Thus, five coordinate, or geometrically strained zinc sites, may represent sites equipped for catalysis, whereas four coordinate ideal tetrahedral zinc sites may represent stable sites affiliated with structural support.
Single-crystal X-ray experiment on samples of 1 after irradiation with UV light (without BG dye) do not show the presence of CO 2 molecules, just coordinated water and small voids with light diffuse electron density. This ruled out the possibility of CO 2 adsorption from air or solvent in 1, and reinforced our hypothesis that CO 2 @1 comes from the decomposition of the BG dye.
The experimental powder X-ray diffraction (PXRD) pattern of a polycrystalline sample of 1 is shown in Figure 5. It is consistent with the theoretical one and confirms the purity and homogeneity of the bulk sample. The solvent contents were determined by thermogravimetric analysis (TGA) under a dry N 2 atmosphere (see Figure S7, Supporting Information) and helped to established the final chemical formula. Attempts to activate 1 (under different protocols) for measuring N 2 isotherms proved unsuccessful, most likely related to its loss of structural stability when all molecule solvents were removed.
The photocatalytic activity of MOF 1 for the degradation of BG dye was then investigated. For this purpose, 25 mg of 1 were suspended in 50 mL of a 10 ppm aqueous solution of BG. Prior to irradiation, the mixture was kept in the dark for 30 min to verify that degradation only occurs under irradiation. After that period, the suspension started to be irradiated, under mild stirring at 250 nm. At different times (5,15,30,60, and 120 min), 1 mL aliquots were taken, centrifuged, and diluted, and their UV-Vis absorption spectra were registered (Figure 6a). An identical experiment was performed, under the same conditions, in the absence of 1 ( Figure S8). In order to have a better characterization of 1, we performed UV-Vis diffuse reflectance spectroscopy, which revealed a strong adsorption band below 350 nm ( Figure S9).   Figure 6 shows the photodegradation efficiency of 1 towards BG. Such efficiency was evaluated by measuring the decrease in the characteristic absorption bands of BG dye, which appear at 420 nm and 625 nm, respectively. Thus, under irradiation in the presence of 1, it a gradual decrease of both peaks with time can be observed, until the vanish completely after 120 min (Figure 6a), which indicates that 100% of BG dye is eliminated after that time (Figure 6b). In turn, the same experiment, in the absence of 1, (Figure S8 and Figure 6b), shows a very smooth decrease of the UV-Vis absorption bands, confirming the key role of 1 as photocatalyst. Tauc plot of the Kubelka-Munk function ( Figure S10) allowed us to obtain an estimation of the optical band of 1 (3.03 eV), which evidenced the suitability of it to perform the degradation of BG. Moreover, the reuse capability of 1 was stablished by both UV-Vis and PXRD experiments. On the one hand, two more UV-Vis vs. time experiments were carried out, using 1 as photocatalyst, with identical results (Figure S11), confirming that 1 can be employed in at least 3 cycles. On the other hand, PXRD patterns of 1 after three cycles are identical to those of the synthesized material suggesting that no degradation occurs during the photocatalytic process (Figure 5c).
The full degradation of the organic dye was also supported by thermogravimetric analysis coupled with mass spectrometry (TGA-MS) on CO 2 @1 ( Figure S12). The weight loss in TGA led to two peaks in MS spectra with the mass to charge ratio (m/z) of 18 and 44, which can be attributed to water and carbon dioxide. However, the amount of CO 2 desorbed was lower than that obtained from X-ray crystallography. Most likely, this is related to some losses of CO 2 during the handling of the measurement itself. The larger size of brilliant green dye compared to 1 pore size precluded the adsorption of the organic dye on MOF channels to perform the photocatalytic event. Thus, the photocatalytic activity of 1 most likely will arise from the crystal surface. With the aim of confirming the photodegradation's occurrance at the surface of 1, we measured the evolution of the BG concentration in solution when it is put in contact with 1 during 30 min in the dark. As can be observed in Figure S13, there is no appreciable difference in the BG concentration in solution, which supports our hypothesis. A leaching test ruled out the possibility of decomposition products of Zn-based MOF being responsible for the photocatalytic degradation of brilliant green. This was also supported, in an indirect manner, by the maintenance of both the activity in the recyclability studies ( Figure S11) and the high crystallinity of PXRD diffraction patterns after the photocatalytic process ( Figure S5c).
A plausible mechanism for the degradation of BG dye is shown in Scheme S1. With the aid of UVC light, 1 generates electron and hole pairs. While the electrons jump to the conducting band, the holes remain in the valence band. The holes were scavenged by water molecules leading to energetically reactive hydroxyl radicals, and the photogenerated electrons react with O 2 to produce the superoxide radical anions of oxygen. From them, based on literature precedents [50][51][52][53], the OH• is believed to be the dominant oxidizing agent for the mineralization of BG dye into CO 2 and H 2 O. 1 H NMR of solutions after photocatalytic experiment do not show any peaks (apart from H 2 O) that can be attributed to any known intermediate from a previously reported study. 55 With the aim of exploring the formation of intermediate species, we performed liquid chromatography/tandem mass spectrometry (LC-MS/MS) on solutions after 30 min. of UV irradiation, where we were able to identify four degradation molecules (Scheme S2) [54].

Conclusions
In summary, we have reported a novel, eco-friendly Zn-based MOF with the formula {Zn 2 [(S,S)-serimox](H 2 O) 2 ] ··· H 2 O (1) and its performance as a photocatalyst of BG aqueous solutions. This MOF with good water stability was able to efficiently photodegrade 10 ppm aqueous solutions of BG in 120 min. Indeed, UV-Vis spectroscopy measurements of irradiated solutions, with and without the presence of 1, clearly revealed the photodegradation role of the framework. Single-crystal X-ray crystallography was applied not only to structurally characterize the pristine structure of 1, but more interestingly, after the photocatalytic process. The resolution of the crystal structure of CO 2 @1 allowed us to confirm the total degradation of the dye and the presence of CO 2 molecules (from the degradation process) retained within the irregular hydrophilic channels of 1. Reusability tests of 1, with up to 3 cycles of the photodegradation process, evidenced structural and performance robustness, which further confirms the viability of 1 as an efficient photocatalyst. Our current work is focused on extending this study to other Zn-based MOFs derived from natural amino acids.

Supplementary Materials:
The following are available online at, experimental preparation, analytical and spectroscopic characterization of 1, and additional Figures S1-S13, Table S1, Schemes S1 and S2.