Biopesticide Encapsulation Using Supercritical CO2: A Comprehensive Review and Potential Applications

As an alternative to synthetic pesticides, natural chemistries from living organisms, are not harmful to nontarget organisms and the environment, can be used as biopesticides, nontarget. However, to reduce the reactivity of active ingredients, avoid undesired reactions, protect from physical stress, and control or lower the release rate, encapsulation processes can be applied to biopesticides. In this review, the advantages and disadvantages of the most common encapsulation processes for biopesticides are discussed. The use of supercritical fluid technology (SFT), mainly carbon dioxide (CO2), to encapsulate biopesticides is highlighted, as they reduce the use of organic solvents, have simpler separation processes, and achieve high-purity particles. This review also presents challenges to be surpassed and the lack of application of SFT for biopesticides in the published literature is discussed to evaluate its potential and prospects.


Introduction
Over 10,000 years ago, in Mesopotamia, edible seeds were stored, marking the advent of agriculture as a way of providing food for the local population [1]. As the crops became bigger, agricultural pests, such as insects, fungi, bacteria, and weeds started to reduce the harvest yield [2]. To prevent those losses, some substances, known as pesticides, were used to control those organisms. Pesticides are any substance or mixture able to repel, eradicate, or mitigate any pest [3]. In the 1940s, during World War II, there was an expansion in synthetic pesticide usage, especially dichloro-diphenyl-trichloroethane (DDT), to limit the spread of malaria vectors, followed by carbamate and other organophosphate pesticides [4]. Since the intensive application of pesticides, agricultural productivity has increased exponentially. According to The Food and Agriculture Organization of the United Nations (FAO) data, approximately 4 million tonnes of pesticides were used globally or sold to the agricultural sector for crops and seeds in 2018.
The negative effects on soil, air, water quality, agricultural products, and nontarget organisms became noticeable, alongside the evolution of pest resistance due to conventional synthetic pesticide use [2,3]. Less harmful alternatives have been used to control pests. As an example, any pest's natural enemy can be used for biocontrol. Before this application, different species of organisms are tested against the target plant pathogen, and the most active species is considered for biocontrol. It also demands extensive knowledge of biocontrol mechanisms (e.g., competition, toxin production, and induction of resistance). Furthermore, properties such as biosafety, production requirements and conditions, and registration issues must be taken into account [5,6]. Water dispersible granule to be dispersed after mixing with water DuPel ® Valent BioSciences [21] Bacillus thuringiensis BT Pests of Orthoptera, Coleoptera, Diptera, Hymenoptera, and especially Lepidoptera.
Water-dispersible powder Bactospeine Xi'an NEO Biotech [22] Bacillus popilliae Japanese beetle grubs. Water-dispersible powder Milky Spore Powder St. Gabriel Organics [23] Chromobacterium subtsugae Armyworms, aphids, Asian citrus psyllid, mites, spotted wing drosophila, thrips and whiteflies.  [31] Despite the low environmental impact of most biopesticides, since they present nontoxic or less toxic constituents, there are some concerns about the commercialization of natural pesticides, such as regulatory requirements, availability of the active ingredient, and stability of the formulation in storage, transport, and during application. To overcome the instability and lower number of active ingredients used, encapsulation technologies have been developed [32,33].

Encapsulation Technologies
Encapsulation is a physical process in which a core material or active substance, such as small solid particles, liquid droplets, or gases are entrapped in an encapsulating agent or wall material to completely or partially isolate the core material, improving its stability, by reducing its volatility and reactivity, and maintaining its viability against adverse environmental conditions, such as pH extremes [11,33,34].
When applied for biomedical purposes, drug encapsulation can reduce toxic side effects, provide drug protection against in vivo degradation and reduce the quantity of active ingredient used, due to its enhanced permeation and retention [34][35][36][37]. In the food industry, recent studies have been focused on encapsulating food-grade active ingredients, such as nutraceuticals, colorings, flavoring agents, enzymes, probiotics, and vitamins [38]. Encapsulation plays a major role in protecting these substances from hazardous external conditions, allowing their transport through the digestive system [39]. It also protects from oxidation reactions and the consequent undesirable taste [11,40]. For agricultural purposes, the process of pesticide encapsulation can reduce human exposure to active ingredients, reduce pesticide residues in agricultural products, increase protection from undesirable reactions and physical stresses, reduce the frequency of application, minimize environmental contamination and prolong the effective duration of non-persistent pesticides [3,7,8,41].
Depending on the materials and the technique used, different structures and morphologies are achieved in the encapsulation process. Solids, liquid droplets, and gas bubbles can be part of the shell material for liquid or gas core. The structure of the encapsulated particles can be spherical, in capsules, beads, monocore, multicore, multishell, or matrix [11,37].
To choose the proper encapsulation technique, the desired application, expected particle size distribution, biocompatibility of the particles, physicochemical properties of the active and the wall material, release mechanism, and process costs are evaluated [11]. There are many encapsulation techniques documented for biopesticides, classified by their chemical or physical processes. Among the chemical processes, the most explored are emulsion polymerization, miniemulsion, melt-dispersion, in situ polymerization, and coacervation. As for the physical processes, spray drying, fluidized bed coating, and ionic gelation encompass the most documented encapsulation techniques for biopesticides and natural substances that have the potential to be biopesticides [7,35,[42][43][44][45] (Table 2). An organic phase is emulsified in an aqueous phase. Polymerization starts with a water-soluble initiator. Fine oil droplets are spontaneously formed when the surfactant moves from the organic phase to the water phase, resulting in oil-in-water (o/w) emulsion [46][47][48]. When an aqueous phase is emulsified in the organic phase of very low polarity, it results in a water-in-oil (w/o) emulsion. This process is referred to as inverse emulsion polymerization [47]. To achieve greater colloidal stability, emulsifiers are added at concentrations equal to or above their critical micelle concentration [35].
There is a relatively poor understanding of factors influencing the encapsulation process and there are limitations on the type of organic phases and surfactants used [46]. In addition, particles present low thermal stability [49].
It is a technique still under improvement without complete knowledge of its mechanism. Additionally, it can present a wide particle size distribution [35]. Chemical Processes

Melt -Dispersion
The active component is melted in water at a temperature above the melting point and emulsified in a high-pressure homogenizer [53].
Depending on the temperature set, it can volatilize core components [54].

nm
Poly-ethylene glycol (PEG) Garlic essential oil [54] In situ polymerization Direct polymerization of a solution of monomers or oligomers is carried out on the core material surface.
Deposition and precipitation are controlled by precipitants or changes in pH and temperature [7,55].

Complex coacervation
This technique relies upon a decrease in solubility of the coating polymer when a third component is added to the polymer solution. Two oppositely charged polymers form a wall around the active ingredient, due to the complexation of oppositely charged polyelectrolytes [44,[58][59][60].
This technique usually requires toxic reagents for the coacervate shell [60].

35-50 µm Gelatin and gum Arabic
Metarhizium anisopliae [61] Physical Processes Spray Drying The core material is homogenized with the carrier, usually an aqueous solution, and then set in a spray dryer Tiny droplets are formed and, by contact with the hot gas, water is evaporated, obtaining a powder or granular product [42,62].
Highly cost equipment and expensive powder recovery process. High heat consumption and low thermal efficiency [63]. In this method, particles with different diameters are moved around in a fluidized bed and sprayed with a liquid. The solution, either aqueous or organic, evaporates and forms a coating layer around the active ingredient [67].
FBC can be applied to a limited range of active ingredients since it degrades temperature-sensitive active compounds [49]. It presents difficulties in processing needle or platelet-shaped particles [68].

-Biomass
Metarhizium brunneum, Cordyceps fumosorosea and Beauveria bassiana [69] Ionic Gelation This technique is based on ionic interactions between charged groups of the polymer and charged groups of the crosslinking agent [70,71].
It can result in nanoparticles and microparticles with a fragile particulate system, high dispersibility index, and few sites to modify the surface for functional moieties attachment [71]. In Table 2, a brief description of each encapsulation method is presented, and there is also information about the material used, particle size, and main disadvantages. Through emulsion polymerization, some natural products with the potential to be biopesticides were exemplified. Chang et al. (2013) [46] encapsulated carvacrol, present in the essential oils of oregano and thyme. In this study, the antimicrobial efficacy of the nanoemulsion was examined, but Campos et al. (2018) [50] explored the toxicity of carvacrol for undesirable invertebrates and nematodes that affect crops. By encapsulating, the low solubility in water of carvacrol was overcome. As the oil concentration increases in the formulation, the storage stability also increases, while the antimicrobial efficacy decreases. This problem can be surpassed by diluting the nanoemulsions before storage. This process was proven to demand low energy and non-complex processes, such as simple stirring. On the other hand, a considerable amount of surfactant is required. Feng et al. (2020) [51] used emulsion polymerization to encapsulate D-limonene, an active ingredient from essential oils of citrus fruits. This substance has documented fungicidal and insecticidal activity, by possibly damaging the cytoplasmic membrane of organisms. D-limonene undergoes oxidative degradation and volatilization so, by encapsulating, it can increase its chemical stability and allow a slow release of this active compound. In this paper, the nanoformulation showed great control of four agricultural pathogens and the fungicidal activity was higher after loading. Though a similar encapsulation technique, demanding more energy, in a process called miniemulsion polymerization, Wahba (2020) [52] encapsulated tea tree oil, grape seed oil, and pomegranate fruit peel oil to evaluate their antifeedant activity against rice weevil (Sitophilus oryzae), which affect stored grains. This method was used to increase the water solubility, permeability and thermal stability of the essential oils without using organic solvents. Since a high-energy emulsification was performed by high shear stirring and ultrasonication, the formulation presented low polydispersity and the droplets did not coalesce. The mortaility of rice weevil was higher after loading.
Garlic essential oil has strong insecticidal activity; however, it has high volatility, strong odor, and low solubility in water. To overcome these drawbacks and increase the insecticidal effectiveness and shelf life, Yang et al. (2009) [54] loaded polyethylene glycol (PEG) with garlic essential oil by melt-dispersion. The formulation was tested against Tribolium castaneum and groups treated with free essential oil presented a rapid decrease in mortality over time, while the nanoparticles presented a gradual decrease in mortality over time. The insecticidal effectiveness was not affected by the 65 • C temperature held in the melt-dispersion process. Though in situ polymerization, Bagle et al. (2013) [56] loaded phenol aldehyde microparticles with neem oil, one of the most effective biopesticides. Neem oil contains azadirachtin, responsible for affecting the behavior and physiology of insects by blocking real hormones from working properly. The encapsulation process was performed to prevent the rapid degradation of neem oil, improve its stability in the environment and allow its slow release. The resulting particles presented spherical shape, with smooth morphology, no agglomeration, and thermal stability.
By complex coacervation, Qiu et al. (2019) [61] entrapped Metarhizium anisopliae, a fungus with insecticidal effects against red imported fire ants (Solenopsis invicta), using gelatin and gum Arabic as wall materials. The microencapsulated conidia presented more resistance to UV exposure and longer lifespan. This can be explained by the mild temperature conditions under which complex coacervation occurs, unlike spray drying and melt-dispersion, preserving the conidia from thermal degradation. Additionally, the layer of gelatin and gum Arabic allows isolation from UV radiation and oxygen. Another fungus, Trichoderma harzianium, used to attack pathogenic plant fungi, was encapsulated. Muñoz-Celaya et al. (2012) [66] loaded maltodextrin and gum Arabic with Trichoderma harzianium conidia. Spray drying was chosen as the method to encapsulate and the use of maltodextrin and gum Arabic was essential to prevent thermal stress during the spray drying operation. This study stated that using the 1:1 blend of maltodextrin-gum Arabic, the best conidia survival rate was achieved (86%). The storage temperature is also important for spore survival. The lowest storage temperature yielded 8 weeks of storage. Pérez-Landa et al. (2020) [64] entrapped the photosensitive biopesticide Spinosad in chitosan/sodium lignosulfonate particles using the spray drying technique. Spinosad acts by disrupting nicotinic acetylcholine receptors of insects but has limitations when used in agriculture since it is photodegradable by sunlight or UV light. In this work, the photostability of Spinosad was enhanced with biopolymers that absorb UV rays. The in vitro results indicated that Spinosad release follows the zero-order kinetic model, with an initial burst effect and then slow release of the active ingredient.
Stephan et al. (2020) [69] investigated fluid-bed coating with liquid fermented biomass containing spores of fungi Metarhizium brunneum, Cordyceps fumosorosea, and Beauveria bassiana approved as an active substance for integrated pest management in Europe. Solidstate fermenters, which are the base for the production of most granules, have high contamination risks and complications for scaleup, so this work focuses on liquid fermented biomass with autoclaved millet as core material and a thin layer of fungal biomass as coating material. The efficacy of these granules was not tested in the field.
Entomopathogenic nematodes (EPN) were also encapsulated. Jaffuel et al. (2020) [72] produced alginate beads containing Heterorhabditis bacteriophora to protect maize plants from Diabrotica balteata larvae. Ionic gelation was used to encapsulate those EPN aiming the increase shelf life and survival after application. Through this technique, an EPNalginate-glycerol solution interacted with a Ca 2+ -glycerol solution. This study tested EPN efficacy to control Diabrotica spp. but it was only conclusive in laboratory tests. Field applications depend on several environmental and application conditions. In the laboratory, the encapsulated EPN presented prolonged shelf life and controlled release, but the larval mortality remained the same as EPN in an aqueous solution.
Among the many encapsulation techniques, there are also those based on supercritical fluid technology (SFT). SFT has many advantages, such as reduced environmental impact, low waste toxicity, and enhanced product quality and safety [9]. This technique stands out in encapsulating thermolabile particles (i.e., those that can be readily destroyed or deactivated by heat) and for creating size-controlled particles [11].

Biopesticide Encapsulation Based On Supercritical Fluid Technology
Above the critical temperature and critical pressure of a fluid, it is named a supercritical fluid, a phase in which there is no transition between vapor and liquid states. The lack of transition between those two phases provides a great solubilizing capability to the supercritical fluid (SCF), which increases with higher pressure values at a constant temperature. SCF refers to a single-phase matter in a non-condensing state near the critical point, which is sensitive to changes in pressure and temperature, once the slightest variation in these parameters leads to noticeable changes in density, resulting in a more gaslike or liquidlike fluid [9,42,74,75].
Compared to other conventional encapsulation processes, the usage of SCF has advantages, such as easy separation of solute/solvent and tunable density. Furthermore, it reduces the use of toxic organic solvents and there is no need to carry out separation steps since this can be performed by depressurization [76,77]. SCF can be used as a solute, solvent or antisolvent, to extract substances from natural products, sterilize products using high-pressure treatment, micronize drugs and encapsulate active substances in a polymeric matrix [74]. The most widely used supercritical fluid in encapsulation processes is supercritical carbon dioxide (scCO 2 ), due to its low toxicity, low cost, non-flammability, easy removal [11], permeability, and mild critical properties (critical temperature, T c = 304.25 K and critical pressure, P c = 7.38 MPa), so it is suitable for processing thermolabile compounds. On the other hand, scCO 2 has some drawbacks, such as non-polarity and solute-solvent interactions, which can be formed due to carbon dioxide's electron donor and acceptor sites [9,78,79].
Some supercritical fluid techniques have been considered to create polymeric micro or nanoparticles of biopesticides with narrow size distribution and high stability, such as Rapid Expansion from Supercritical Solution (RESS), Particle from Gas Saturated Solutions (PGSS), Supercritical Solvent Impregnation (SSI) Supercritical Assisted Atomization (SAA) and Supercritical Phase Inversion [74,77,[80][81][82]. Table 3 illustrates some applications of supercritical technologies applied to biopesticides or natural products that could be potential biopesticides.

Rapid Expansion from Supercritical Solution (RESS)
Through Rapid Expansion from Supercritical Solution (RESS), fine particles can be produced by saturating the SCF with a solid in an extraction unit. Then, the solution is abruptly depressurized in a low-pressure chamber, leading to rapid nucleation of the substrate in fine particles, collected from the gaseous stream [74,93] (Figure 1). The morphology of the resulting solid material depends on the temperature, pressure drop, nozzle geometry, and other parameters, as well as on the chemical structure of the active ingredient [93].  This method has been explored for a wide range of materials, including polymers, dyes, medicines, and inorganic substances [74]. Using CO2 as a solvent, ethanol as a cosolvent, and polyethyleneglycol (PEG) as encapsulant material, Santos et al. (2013) [80] studied the encapsulation of anthocyanins extracted from jaboticaba skins. The encapsulation was carried out by the RESS process, and the optimal condition was at a temperature of 40 °C and pressure of 20 MPa.
Using the RESS process it is possible to encapsulate in liposomes an essential oil extracted from the rhizome of Atractylodes macrocephala, a traditional Chinese medicine (Table 3) [83]. This essential oil was dissolved in a mixture containing scCO2 and ethanol. As a result, particles were obtained with an average diameter of 173 nm and 82.18% of entrapment efficiency. Even though it was not the application in the previous work by Wen Although RESS has been used to create films, nano, and microparticles, it is difficult to control the particle size of precipitates, due to the particle coalescence in the supersonic free jet generated in the precipitation vessel. To avoid the formation of liquid droplets, it is necessary to have previous knowledge of the variation of solid/polymer melting temperature with the pressure. Therefore, it is possible to determine the solubility of the particles in the SCF [78], since this process is suitable only for particles that are soluble in the supercritical fluid, excluding solid active compounds with high molecular weight and polar bonds, since they have low solubility in scCO 2 [93,94]. This method has been explored for a wide range of materials, including polymers, dyes, medicines, and inorganic substances [74]. Using CO 2 as a solvent, ethanol as a cosolvent, and polyethyleneglycol (PEG) as encapsulant material, Santos et al. (2013) [80] studied the encapsulation of anthocyanins extracted from jaboticaba skins. The encapsulation was carried out by the RESS process, and the optimal condition was at a temperature of 40 • C and pressure of 20 MPa.
Using the RESS process it is possible to encapsulate in liposomes an essential oil extracted from the rhizome of Atractylodes macrocephala, a traditional Chinese medicine (Table 3) [83]. This essential oil was dissolved in a mixture containing scCO 2 [84] paper, some compounds of this oil presented pesticide effects against the common vinegar fly, Drosophila melanogaster L., suggesting that the potential of this oil as biopesticide can be assessed in further studies.

Particle from Gas Saturated Solutions (PGSS)
Particle from Gas Saturated Solutions (PGSS) is a process in which an SCF, usually scCO 2 , is dissolved in different materials of low melting temperatures. This technique has been used for some liquids and solids, such as powder coatings, monoglycerides, vitamins, antioxidants, fats, and food-related products [85]. The process is schematized in Figure 2. The solution or melt to be crystallized is mixed and saturated with the SCF at an appropriate pressure and temperature, and the solution is sprayed into a low-pressure precipitation chamber. After the depressurization, the dissolved carbon dioxide is released and expands; consequently, there is a cooling effect that promotes the formation of the microparticles [42,95]. This method has been explored for a wide range of materials, including polymers, dyes, medicines, and inorganic substances [74]. Using CO2 as a solvent, ethanol as a cosolvent, and polyethyleneglycol (PEG) as encapsulant material, Santos et al. (2013) [80] studied the encapsulation of anthocyanins extracted from jaboticaba skins. The encapsulation was carried out by the RESS process, and the optimal condition was at a temperature of 40 °C and pressure of 20 MPa.
Using the RESS process it is possible to encapsulate in liposomes an essential oil extracted from the rhizome of Atractylodes macrocephala, a traditional Chinese medicine (Table 3) [83]. This essential oil was dissolved in a mixture containing scCO2 and ethanol. As a result, particles were obtained with an average diameter of 173 nm and 82.18% of entrapment efficiency. Even though it was not the application in the previous work by Wen et al. (2010) [83], in Chu et al.'s (2011) [84] paper, some compounds of this oil presented pesticide effects against the common vinegar fly, Drosophila melanogaster L., suggesting that the potential of this oil as biopesticide can be assessed in further studies.

Particle from Gas Saturated Solutions (PGSS)
Particle from Gas Saturated Solutions (PGSS) is a process in which an SCF, usually scCO2, is dissolved in different materials of low melting temperatures. This technique has been used for some liquids and solids, such as powder coatings, monoglycerides, vitamins, antioxidants, fats, and food-related products [85]. The process is schematized in Figure 2. The solution or melt to be crystallized is mixed and saturated with the SCF at an appropriate pressure and temperature, and the solution is sprayed into a low-pressure precipitation chamber. After the depressurization, the dissolved carbon dioxide is released and expands; consequently, there is a cooling effect that promotes the formation of the microparticles [42,95].  [86] encapsulated lavender essential oil in PEG and octenyl succinic anhydride (OSA) starch, a chemically modified starch. Before processing in the PGSS plant, emulsion water in oil was prepared. PEG microcapsules achieved high encapsulation efficiency, ranging from 14 to 66%. Particles were spherical and a narrow particle size distribution was present of 21 to 49 µm. No data on the active ingredient release profile was obtained.

Supercritical Solvent Impregnation (SSI)
Using Supercritical Solvent Impregnation (SSI), it is possible to impregnate polymers with active compounds dissolved in supercritical fluids, especially scCO 2 . It is also possible to dissolve other substances to enhance the solubility of the active compound, acting as a cosolvent, or add a surfactant to improve its dispersion in the polymer [42].
Divided into three steps, the SSI process starts with the dissolution of active substances in SCF. After that, the supercritical fluid containing the active ingredient swells into a polymeric matrix, promoting the internal diffusion of the active compound followed by decompression, lowering the SCF density and allowing solute precipitation [96] (Figure 3). tion efficiency, ranging from 14 to 66%. Particles were spherical and a narrow particle size distribution was present of 21 to 49 µm. No data on the active ingredient release profile was obtained.

Supercritical Solvent Impregnation (SSI)
Using Supercritical Solvent Impregnation (SSI), it is possible to impregnate polymers with active compounds dissolved in supercritical fluids, especially scCO2. It is also possible to dissolve other substances to enhance the solubility of the active compound, acting as a cosolvent, or add a surfactant to improve its dispersion in the polymer [42].
Divided into three steps, the SSI process starts with the dissolution of active substances in SCF. After that, the supercritical fluid containing the active ingredient swells into a polymeric matrix, promoting the internal diffusion of the active compound followed by decompression, lowering the SCF density and allowing solute precipitation [96] ( Figure 3).  [88] incorporated two terpenic ketones, thymoquinone and R-(+)pulegone, in low-density polyethylene (LDPE) by the impregnation of a supercritical solvent with CO2. The impregnated films presented a ketone concentration ranging from 2.25 to 5.59% (w/w) and a high insecticidal effect was obtained, with nearly 85% mortality against the weevil Sitophilus zeamais, which fell to 20-30% over the next seven days.

Supercritical Assisted Atomization (SAA)
In the Supercritical Assisted Atomization (SAA) process, scCO2 acts as a cosolute and is dissolved in a solution containing the active compound, forming an expanded liquid solution with reduced viscosity and surface tension, reducing cohesive forces [90]. This is a two-step atomization: first, there is pneumatic atomization, in which a pressure drop occurs at the nozzle outlet, and then there is decompressive atomization, by steadily delivering CO2 in the primary droplets. The microparticles are formed through the evaporation of the solvent and supersaturation of the solute in the droplets (Figure 4) [97].  [88] incorporated two terpenic ketones, thymoquinone and R-(+)pulegone, in low-density polyethylene (LDPE) by the impregnation of a supercritical solvent with CO 2 . The impregnated films presented a ketone concentration ranging from 2.25 to 5.59% (w/w) and a high insecticidal effect was obtained, with nearly 85% mortality against the weevil Sitophilus zeamais, which fell to 20-30% over the next seven days.

Supercritical Assisted Atomization (SAA)
In the Supercritical Assisted Atomization (SAA) process, scCO 2 acts as a cosolute and is dissolved in a solution containing the active compound, forming an expanded liquid solution with reduced viscosity and surface tension, reducing cohesive forces [90]. This is a two-step atomization: first, there is pneumatic atomization, in which a pressure drop occurs at the nozzle outlet, and then there is decompressive atomization, by steadily delivering CO 2 in the primary droplets. The microparticles are formed through the evaporation of the solvent and supersaturation of the solute in the droplets (Figure 4) [97]. This technique has been used with different compounds: active molecules, proteins, and polymers [81]. SAA can be applied with an organic and aqueous solvent, and it is a flexible and easily scalable process [97]. Even though this process presents some limitations for thermolabile compounds due to the high temperature required, this problem can be overcome by applying a vacuum in the precipitator [78]. Santo et al. (2014) [98] prepared liposomes from droplets formed by the atomization of an expanded liquid mixture of phospholipids, ethanol, and carbon dioxide. These droplets were coated by a lipid layer, resulting in a water-in-CO 2 emulsion, which was transformed into liposomes by precipitation in the water pool at the bottom of the vessel. The mixer and vessel pressure varied from 12.5 to 17.5 MPa, and the temperature was set at 70 • C. It was possible to form particles with sizes ranging between 130 ± 62 and 294 ± 144 nm and encapsulation efficiencies from 85 to 90% were obtained. Adami et al. (2011) [99] produced microparticles of two thermolabile compounds: bovine serum albumin (BSA) and poly L-lactide (PLLA). Spherical particles were obtained with sizes ranging from 1 to 1.5 µm. The precipitation pressure was 0.05 to 0.065 MPa to avoid coalescence and the saturation pressure ranged from 8 to 10.5 MPa. The precipitation occurred from 30 to 80 • C, and the saturator temperature fluctuated between 60 and 81 • C. Using polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP) as biopolymers, rotenone was encapsulated by the SAA process in Martin et al.'s (2013) work [90]. Rotenone is an organic molecule that occurs naturally from the seeds and stems of several plants, such as Derri elliptica and Tephrosia vogelii. The spherical particles obtained had a mean diameter varying from 0.6 to 1.5 µm with an encapsulation efficiency near 100%. This technique has been used with different compounds: active molecules, proteins, and polymers [81]. SAA can be applied with an organic and aqueous solvent, and it is a flexible and easily scalable process [97]. Even though this process presents some limitations for thermolabile compounds due to the high temperature required, this problem can be overcome by applying a vacuum in the precipitator [78]. Santo et al. (2014) [98] prepared liposomes from droplets formed by the atomization of an expanded liquid mixture of phospholipids, ethanol, and carbon dioxide. These droplets were coated by a lipid layer, resulting in a water-in-CO2 emulsion, which was transformed into liposomes by precipitation in the water pool at the bottom of the vessel. The mixer and vessel pressure varied from 12.5 to 17.5 MPa, and the temperature was set at 70°C. It was possible to form particles with sizes ranging between 130 ± 62 and 294 ± 144 nm and encapsulation efficiencies from 85 to 90% were obtained. Adami et al. (2011) [99] produced microparticles of two thermolabile compounds: bovine serum albumin (BSA) and poly L-lactide (PLLA). Spherical particles were obtained with sizes ranging from 1 to 1.5 µm. The precipitation pressure was 0.05 to 0.065 MPa to avoid coalescence and the saturation pressure ranged from 8 to 10.5 MPa. The precipitation occurred from 30 to 80 °C, and the saturator temperature fluctuated between 60 and 81 °C. Using polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP) as biopolymers, rotenone was encapsulated by the SAA process in Martin et al.'s (2013) work [90]. Rotenone is an organic molecule that occurs naturally from the seeds and stems of several plants, such as Derri elliptica and Tephrosia vogelii. The spherical particles obtained had a mean diameter varying from 0.6 to 1.5 µm with an encapsulation efficiency near 100%.

Supercritical Antisolvent Fractionation (SAF)
Through Supercritical Antisolvent Fractionation (SAF), the SCF, mainly scCO2, is used as an antisolvent: it dissolves the organic solvent and eliminates undesired compounds. As a result, there is an enrichment of the active ingredient [91].
Since scCO2 has a non-polar nature, it is possible to fractionate polar compounds of interest in an organic solution containing various components. This happens during the contact between the SCF and the liquid mixture in a pressurized vessel. The liquid solu-

Supercritical Antisolvent Fractionation (SAF)
Through Supercritical Antisolvent Fractionation (SAF), the SCF, mainly scCO 2 , is used as an antisolvent: it dissolves the organic solvent and eliminates undesired compounds. As a result, there is an enrichment of the active ingredient [91].
Since scCO 2 has a non-polar nature, it is possible to fractionate polar compounds of interest in an organic solution containing various components. This happens during the contact between the SCF and the liquid mixture in a pressurized vessel. The liquid solution, dispersed via spray, can be fractionated and precipitated in a high-pressure vessel. CO 2 is recovered by decompression [76,91]

Supercritical Phase Inversion and Supercritical Drying
Supercritical Phase Inversion offers the possibility to create porous membranes of polymers that can be loaded with active ingredients. In this technique, a liquid-liquid demixing phenomenon is produced from a polymeric solution by using scCO2 as a nonsolvent to steadily remove the organic solvent. The use of scCO2 in this phase inversion avoids the collapse of the pore structure due to its low surface tension and gaslike diffusivity. Moreover, a subsequent membrane drying process is not required. The membrane morphology can be controlled by changing the operating conditions [100] and compounds can be included in the membrane. This process starts with producing polymer solutions by solubilizing the polymer in an organic solvent that should have a proper affinity with scCO2. The final solution is placed in stainless steel caps and processed in a vessel containing scCO2, filled from the bottom up to the desired pressure. Then, this vessel is depressurized, forming dried membranes [101]. Baldino et al. (2021) [92] used quercetin, a plant flavonoid, as a fungicide against Kluyveromyces lactis and Yarrowia lipolitica. Membranes of cellulose acetate were produced by supercritical phase inversion to load quercetin with different polymer concentrations (5%, 10%, and 15% w/w), pressures (10 and 20 MPa), and temperatures (45 and 50 °C). Aerogels are ultralight materials with high porosity. This type of material is obtained by drying a gel. The gel can be produced by different gelation methodologies in bead-or monolithlike form, and from different inorganic or organic compounds. After that, the gel must be dried with any technique. In this context, the use of scCO2 helps to preserve the gel's internal network due to its low surface tension. The affinity of the solvent and scCO2 must be high. Then, if a hydrogel has to be dried (water-scCO2 affinity is low), a multistep solvent exchange must be performed with a different organic solvent before the drying process to obtain an alcogel [102][103][104].
Aerogels can be loaded with any compound in different steps. The molecule can be included in the precursor solution, during the gel formation, in the alcogel, during the

Supercritical Phase Inversion and Supercritical Drying
Supercritical Phase Inversion offers the possibility to create porous membranes of polymers that can be loaded with active ingredients. In this technique, a liquid-liquid demixing phenomenon is produced from a polymeric solution by using scCO 2 as a non-solvent to steadily remove the organic solvent. The use of scCO 2 in this phase inversion avoids the collapse of the pore structure due to its low surface tension and gaslike diffusivity. Moreover, a subsequent membrane drying process is not required. The membrane morphology can be controlled by changing the operating conditions [100] and compounds can be included in the membrane. This process starts with producing polymer solutions by solubilizing the polymer in an organic solvent that should have a proper affinity with scCO 2 . The final solution is placed in stainless steel caps and processed in a vessel containing scCO 2 , filled from the bottom up to the desired pressure. Then, this vessel is depressurized, forming dried membranes [101]. Baldino et al. (2021) [92] used quercetin, a plant flavonoid, as a fungicide against Kluyveromyces lactis and Yarrowia lipolitica. Membranes of cellulose acetate were produced by supercritical phase inversion to load quercetin with different polymer concentrations (5%, 10%, and 15% w/w), pressures (10 and 20 MPa), and temperatures (45 and 50 • C). Aerogels are ultralight materials with high porosity. This type of material is obtained by drying a gel. The gel can be produced by different gelation methodologies in bead-or monolithlike form, and from different inorganic or organic compounds. After that, the gel must be dried with any technique. In this context, the use of scCO 2 helps to preserve the gel's internal network due to its low surface tension. The affinity of the solvent and scCO 2 must be high. Then, if a hydrogel has to be dried (water-scCO 2 affinity is low), a multistep solvent exchange must be performed with a different organic solvent before the drying process to obtain an alcogel [102][103][104].
Aerogels can be loaded with any compound in different steps. The molecule can be included in the precursor solution, during the gel formation, in the alcogel, during the supercritical drying, or even after obtaining the aerogel by any impregnation methodology, such as supercritical impregnation. These phenomena have been reviewed in [103,105,106]. Registered by the number US7674476B1, this patent is related to the use of aerogels as a carrier for active ingredients in agriculture and/or veterinary medicine. The active materials can be insecticides, herbicides, fungicides, acaricides, rodenticides, piscicides, molluscicides, nematicides, bactericides, and/or parasiticides [107].

Supercritical Anti-Solvent Precipitation (SAS)
A potential SFT for biopesticide encapsulation is Supercritical Anti-Solvent Precipitation (SAS), in which scCO 2 is used as antisolvent, so it has to be miscible with the chosen liquid solvent. This method aims to create a free-flowing powder, and a polymer can be added to the solution to encapsulate the particles [76]. SAS treated material can range from nanoparticles to microparticles and it can be amorphous or semi-crystalline [108].
In this process, CO 2 is pumped at a constant rate to the precipitation chamber until it reaches the desired pressure ( Figure 5). Then, the temperature is set and the pure solvent is sent to the precipitator through a nozzle. After that, the liquid solution containing the active ingredient dissolved in the selected solvent is injected. As a result of the supersaturation, solute precipitation occurs on a filter, while the solvent/antisolvent mixture is recovered. The use of scCO 2 eliminates the solvent residues. Finally, after the washing step, there is a depressurization in the precipitator to atmospheric pressure to collect the resulting powder [82,109,110].
Compared to other techniques such as RESS and spray-drying, the particles formed by SAS present a narrower particle size distribution and higher specific surface area [110]. Among the limitations of SAS, some stand out, such as the difficulty in working with molecules soluble in CO 2 or the solvent/antisolvent mixture, and with hydrophilic compounds, since there is a notable difference between water and CO 2 solubility in SAS conditions [82]. Oliveira et al. (2017) [111] encapsulated passion fruit seed oil in poly(lactic-co-glycolic) acid (PLGA) by the SAS method to maintain the antioxidant and antimicrobial activities. The organic phase, containing oil, PLGA, and dichloromethane, and CO 2 were fed into the precipitation chamber, where fast solubilization of the organic solvent in CO 2 occurred, precipitating the oil/PLGA mixture. After depressurization, the particles collected presented a size distribution from 721 to 1498 nm, with the entrapment efficiency varying from 67.8 to 91%. Differential Scanning Calorimetry (DSC) tests showed that lower temperatures (35 • C) prevented thermal degradation of the oil.
Although there is a lack of biopesticide encapsulation using supercritical technology in the literature, SFT could be applied to compounds that were encapsulated by other methods to explore their advantages. As an example, it is possible to evaluate the possibility of creating an inclusion complex of pesticides with cyclodextrins by the SAS technique, since there is a previous study [112] using cyclodextrins to extract pesticides from the soil.

Challenges of Supercritical Fluid Technologies
One of the main challenges of using SFT is the solubility or insolubility between the active substance and the carrier material in the supercritical fluid, especially CO 2 because it indicates the amount of active compound that can be entrapped in the process. Once the solubility data is well-known, the shelf life can be assessed, since it is possible to understand the susceptibility of the active compound to degradation processes by segregation out of the carrier [45].
The phase equilibria modeling under pressure for SCF has not presented good predictability, especially when multiple phases, such as cosolvents and polymeric carriers, are evolved in the operation. Therefore, it is necessary to have extensive experimental data available to scale up and allow these techniques to be reproducible [77].
Although supercritical fluid techniques for particle formation have been successfully used in research for many years, there are still many issues concerning their scaleup. In atomization processes, the length-to-diameter ratio and fluid velocity are very important to determine the final particle size. In this context, different approaches can be followed. The first one is the use of several plants with the same dimensions (that is, numberingup), but that approach involves different drawbacks, such as keeping the same pressure drop in every nozzle or avoiding nozzle blockage due to the Joule-Thomson effect in every piece of equipment. Therefore, it seems more appropriate to use only one device, keeping constant the governing process dimensionless numbers, such as Reynolds, Weber, or Ohnesorge, and as a consequence, the different governing forces involved will be controlled [113,114].
The following issue concerns particle recovery. In this case, filtration is the more adequate technique, but with some problems, such as harvesting from the filters or even the residence time. This drawback can be resolved by using more collection filters, as was proposed by Clavier and Perrut [115]. Finally, it is important to design a recycling system to recover the used supercritical fluid, and a process separation step should always be included to remove cosolvents, antisolvents, or in this topic, pesticide traces. This issue is related to every supercritical fluid technique (drying with supercritical fluids or for particle formation).
Perhaps the most important thing is to perform the scaleup, fulfilling the Good Manufacturing Practices (GMPs). Supercritical fluid techniques are high-pressure processes with a high risk. Therefore, automatization systems, the use of "clean" rooms for the vessels, and fluid management should be taken into account. Moreover, special care has to be taken concerning blockages of pipes or nozzles due to the Joule-Thomson effect, as well as with leaks.
Although it is difficult to fulfill all the previously explained issues, it should be taken into account that extraction processes with scCO 2 are already used on an industrial scale and that some APIs (Formulcoat, Formulplex, and Formuldisp) are already under pre-formulation [116].

Conclusions
Biopesticides have gained recognition in the marketplace, since they present several advantages, such as less toxicity to crops and nontarget organisms compared to extensively used synthetic pesticides. However, regulatory requirements, the stability of the formulation, availability, and standardization of the active compound limit their commercial success, which is reflected in the tiny selection of products presented in this review.
Regarding the physical and chemical stability of the active ingredients of biopesticides, this work summarized the most common encapsulation techniques, highlighting the materials used, particle size, and their effect on the stability of the active compound. Supercritical fluid technology, especially that using supercritical carbon dioxide, stands out among other encapsulation technologies, due to the reduction in toxic organic solvents and ease of separation by depressurization.
Challenges regarding the use of supercritical fluid technologies were presented, such as solubility in the SCF, scaleup, multiphase equilibria, and product recovery concerns. Furthermore, studies must be carried out to investigate if encapsulation processes can damage biopesticide activity, especially under supercritical conditions.